Rh2AG480200

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015738
Physical Location & Seq
Forward (+)
69976347 .. 69981236
4890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG480200.1

Sequence Viewer

Length: 1092 bp
ATGCTAAAGAAGAAGCTAGAAGAAGTTGATCCATTAGATCAAAGGTGGTCCCCTTGTCTTTCTTGGTCAATGGGGTCTATAGACGAGAAGATAGACCTTGAAGCTCATGAGGAGCTGACATTTGGCTCATCAGCAGCCACGGGTGACATAGAGGCCCTGCATTTTGAGGCGGTTCATTTGCACTCCCGAGCTGATGTGGCATACTCATGGATTTCGAAGAATGTTGGGAGGATAAGAACTCCGAGGAGCCTTTATGTTGTCCTTTTGAATGCAAAGATCACCATGTTGCTACCCTGTGGTCCCTTGGCAATTTGGCTACATATCGTTACTGAAAAGCAGGGATGGGTCCTCTTCTTCAGCTTATTGGGCACGGCACCATTAACAGAGCGTTTGGGATATGTGACTGGTAAGCAACTCGCCTTGCACTCAGAACCGAGAGTTGGGGGACTTATAAATGTCACATTTGTAACTGTACCTCAGATCTTTTCATCACTCTTTGCATTAAGGAAAGACATGATTAGAGTTCAACACGCCCTCGTGAACGTTACATTGTTGTCAATGGCTTTAATGGGGATTATGCTTCCAGCTGTGCTTCACTTTAAGTTCTTTAAGTTTATAAATCATCATGATCGTCATAGTCCCATTCATGAGGATGAGGATGAGGAAGGAATCCAAGATCAAGATTCTTCTCCGGAAATAACTACTAATGAAGCAATTGGCTGGCTTTCTATCTTAGCTTTGTGGGTGTACATTTTGTCTTCATACCTTGTGGAGGCTATACAGGGAGCATCCGACTCGCTCAATATTCCTGTAGCATTTATCAATGCCATCCTGCTCCCACTTGTAGGAAATATGTGGTCTATCCTGTTTTCCATGAGAGAAAAACTTGACCTCACGCTCAGAGTCGCTATTGGATTATCTACACAAACATCTATGTTTACAATCCCCTTTTGTGTAATTGTTGGGTGGATAATAGGAAATCCTATGGACTTGAATTTTCAACTCTTTGAGACTGTTATATTGGTTATGGCTGTGCTAGTAGTGGCATTTATGTTGCAGGTAATGTCGTCAATAGTAGTGAAAGATGCATGA

Protein Analysis

363

Amino Acids

40.58

Weight (kDa)

5.52

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 239 - 352 1.9e-08 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 452, 617
Acc36I ACCTGC 1 cut(s) 1048
AccB1I GGYRCC 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 691
AciI CCGC 1 cut(s) 170
AclI AACGTT 1 cut(s) 543
AclWI GGATC 1 cut(s) 23
AcsI RAATTY 1 cut(s) 994
AcuI CTGAAG 1 cut(s) 340
AfaI GTAC 2 cut(s) 474, 749
AfiI CCNNNNNNNGG 3 cut(s) 440, 772, 845
AgsI TTSAA 5 cut(s) 101, 268, 527, 994, 1001
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 7 cut(s) 16, 104, 115, 191, 360, 587, 737
AluI AGCT 7 cut(s) 16, 104, 115, 191, 360, 587, 737
Alw26I GTCTC 1 cut(s) 1004
AlwI GGATC 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 186
Aor13HI TCCGGA 1 cut(s) 691
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 994
AspS9I GGNCC 4 cut(s) 48, 154, 299, 346
AsuHPI GGTGA 2 cut(s) 155, 271
AsuII TTCGAA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 3 cut(s) 48, 299, 346
BaeGI GKGCMC 1 cut(s) 371
BanI GGYRCC 1 cut(s) 373
BauI CACGAG 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 750
BbvI GCAGC 1 cut(s) 146
BccI CCATC 2 cut(s) 336, 836
BceAI ACGGC 1 cut(s) 387
BcgI CGANNNNNNTGC 2 cut(s) 777, 811
BcoDI GTCTC 1 cut(s) 1004
BfaI CTAG 2 cut(s) 17, 1037
BfmI CTRYAG 2 cut(s) 78, 810
BfuAI ACCTGC 1 cut(s) 1048
BglII AGATCT 1 cut(s) 480
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme18I GGWCC 3 cut(s) 48, 299, 346
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 4 cut(s) 48, 154, 299, 346
BmiI GGNNCC 5 cut(s) 50, 248, 301, 347, 375
BmsI GCATC 2 cut(s) 797, 1075
BpiI GAAGAC 1 cut(s) 750
Bpu14I TTCGAA 1 cut(s) 215
BsaJI CCNNGG 3 cut(s) 138, 242, 303
BsaWI WCCGGW 1 cut(s) 691
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 772, 845
Bse1I ACTGG 1 cut(s) 409
BseAI TCCGGA 1 cut(s) 691
BseDI CCNNGG 3 cut(s) 138, 242, 303
BseGI GGATG 5 cut(s) 347, 658, 664, 788, 828
BseLI CCNNNNNNNGG 3 cut(s) 440, 772, 845
BseMII CTCAG 3 cut(s) 441, 491, 913
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 2 cut(s) 125, 259
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 1 cut(s) 146
BshFI GGCC 1 cut(s) 155
BshNI GGYRCC 1 cut(s) 373
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 1 cut(s) 692
BslFI GGGAC 4 cut(s) 34, 285, 459, 624
BslI CCNNNNNNNGG 3 cut(s) 440, 772, 845
BsmAI GTCTC 1 cut(s) 1004
BsmFI GGGAC 4 cut(s) 34, 285, 459, 624
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 1 cut(s) 155
BsoBI CYCGRG 1 cut(s) 186
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 371
Bsp13I TCCGGA 1 cut(s) 691
Bsp1407I TGTACA 1 cut(s) 747
Bsp143I GATC 6 cut(s) 28, 37, 276, 480, 628, 676
BspACI CCGC 1 cut(s) 170
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 3 cut(s) 440, 490, 912
BspEI TCCGGA 1 cut(s) 691
BspHI TCATGA 3 cut(s) 106, 625, 646
BspLI GGNNCC 5 cut(s) 50, 248, 301, 347, 375
BspMI ACCTGC 1 cut(s) 1048
BspPI GGATC 1 cut(s) 23
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 373
BsrGI TGTACA 1 cut(s) 747
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 3 cut(s) 138, 242, 303
BssMI GATC 6 cut(s) 28, 37, 276, 480, 628, 676
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 536
Bst4CI ACNGT 2 cut(s) 472, 1015
Bst6I CTCTTC 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 747
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 722
BstDEI CTNAG 4 cut(s) 427, 477, 733, 899
BstDSI CCRYGG 1 cut(s) 138
BstENI CCTNNNNNAGG 1 cut(s) 770
BstF5I GGATG 5 cut(s) 347, 658, 664, 788, 828
BstKTI GATC 6 cut(s) 31, 40, 279, 483, 631, 679
BstMAI GTCTC 1 cut(s) 1004
BstMBI GATC 6 cut(s) 28, 37, 276, 480, 628, 676
BstMWI GCNNNNNNNGC 2 cut(s) 197, 366
BstSFI CTRYAG 2 cut(s) 78, 810
BstSLI GKGCMC 1 cut(s) 371
BstV1I GCAGC 1 cut(s) 146
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 1 cut(s) 480
BstYI RGATCY 1 cut(s) 480
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 5 cut(s) 347, 658, 664, 788, 828
BveI ACCTGC 1 cut(s) 1048
Cac8I GCNNGC 1 cut(s) 722
CciI TCATGA 3 cut(s) 106, 625, 646
Cfr13I GGNCC 4 cut(s) 48, 154, 299, 346
Csp6I GTAC 2 cut(s) 473, 748
CviAII CATG 8 cut(s) 107, 207, 283, 514, 626, 647, 874, 1089
CviQI GTAC 2 cut(s) 473, 748
DdeI CTNAG 4 cut(s) 427, 477, 733, 899
DpnI GATC 6 cut(s) 30, 39, 278, 482, 630, 678
DpnII GATC 6 cut(s) 28, 37, 276, 480, 628, 676
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco130I CCWWGG 1 cut(s) 303
Eco47I GGWCC 3 cut(s) 48, 299, 346
Eco57I CTGAAG 1 cut(s) 340
Eco88I CYCGRG 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 770
EcoO109I RGGNCCY 2 cut(s) 154, 346
EcoT14I CCWWGG 1 cut(s) 303
EcoT22I ATGCAT 1 cut(s) 1090
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 8 cut(s) 110, 210, 286, 517, 629, 650, 877, 1092
FaqI GGGAC 4 cut(s) 34, 285, 459, 624
FatI CATG 8 cut(s) 106, 206, 282, 513, 625, 646, 873, 1088
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 5 cut(s) 354, 665, 671, 775, 815
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 2 cut(s) 17, 1037
GluI GCNGC 1 cut(s) 135
HaeIII GGCC 1 cut(s) 155
HapII CCGG 1 cut(s) 692
Hin1II CATG 8 cut(s) 110, 210, 286, 517, 629, 650, 877, 1092
HinfI GANTC 4 cut(s) 669, 683, 794, 903
HpaII CCGG 1 cut(s) 692
HphI GGTGA 2 cut(s) 155, 271
Hpy166II GTNNAC 3 cut(s) 541, 748, 939
Hpy188I TCNGA 5 cut(s) 243, 430, 480, 793, 902
Hpy188III TCNNGA 7 cut(s) 107, 186, 538, 626, 647, 680, 692
Hpy8I GTNNAC 3 cut(s) 541, 748, 939
HpyAV CCTTC 1 cut(s) 659
HpyCH4III ACNGT 2 cut(s) 472, 1015
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 7 cut(s) 160, 181, 272, 424, 500, 1057, 1088
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 366
HpyF3I CTNAG 4 cut(s) 427, 477, 733, 899
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 8 cut(s) 110, 210, 286, 517, 629, 650, 877, 1092
Kpn2I TCCGGA 1 cut(s) 691
Kzo9I GATC 6 cut(s) 28, 37, 276, 480, 628, 676
LmnI GCTCC 4 cut(s) 112, 246, 785, 840
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 2 cut(s) 797, 1075
MaeI CTAG 2 cut(s) 17, 1037
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 6 cut(s) 143, 325, 400, 457, 466, 544
MalI GATC 6 cut(s) 30, 39, 278, 482, 630, 678
MboI GATC 6 cut(s) 28, 37, 276, 480, 628, 676
MboII GAAGA 8 cut(s) 22, 32, 100, 229, 343, 346, 678, 750
MfeI CAATTG 1 cut(s) 714
MflI RGATCY 1 cut(s) 480
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 4 cut(s) 309, 714, 957, 994
MlyI GAGTC 2 cut(s) 788, 912
MmeI TCCRAC 1 cut(s) 816
Mph1103I ATGCAT 1 cut(s) 1090
MroI TCCGGA 1 cut(s) 691
MseI TTAA 5 cut(s) 380, 503, 566, 600, 609
MslI CAYNNNNRTG 2 cut(s) 205, 651
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 1 cut(s) 692
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 274
MwoI GCNNNNNNNGC 2 cut(s) 197, 366
NdeII GATC 6 cut(s) 28, 37, 276, 480, 628, 676
NlaIII CATG 8 cut(s) 110, 210, 286, 517, 629, 650, 877, 1092
NlaIV GGNNCC 5 cut(s) 50, 248, 301, 347, 375
NmuCI GTSAC 3 cut(s) 143, 400, 457
NsiI ATGCAT 1 cut(s) 1090
NspV TTCGAA 1 cut(s) 215
PagI TCATGA 3 cut(s) 106, 625, 646
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 669, 683
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 2 cut(s) 788, 911
PpsI GAGTC 2 cut(s) 788, 911
PpuMI RGGWCCY 1 cut(s) 346
PsiI TTATAA 2 cut(s) 452, 617
Psp1406I AACGTT 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 346
PspN4I GGNNCC 5 cut(s) 50, 248, 301, 347, 375
PspPI GGNCC 4 cut(s) 48, 154, 299, 346
PspPPI RGGWCCY 1 cut(s) 346
PsuI RGATCY 1 cut(s) 480
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 2 cut(s) 474, 749
RsaNI GTAC 2 cut(s) 473, 748
RseI CAYNNNNRTG 2 cut(s) 205, 651
SaqAI TTAA 5 cut(s) 380, 503, 566, 600, 609
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 6 cut(s) 28, 37, 276, 480, 628, 676
Sau96I GGNCC 4 cut(s) 48, 154, 299, 346
SchI GAGTC 2 cut(s) 788, 912
SduI GDGCHC 1 cut(s) 371
SfaNI GCATC 2 cut(s) 797, 1075
SfcI CTRYAG 2 cut(s) 78, 810
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 3 cut(s) 48, 299, 346
SmiMI CAYNNNNRTG 2 cut(s) 205, 651
Sse9I AATT 4 cut(s) 309, 714, 957, 994
SsiI CCGC 1 cut(s) 170
SspI AATATT 1 cut(s) 805
SspMI CTAG 2 cut(s) 17, 1037
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 472, 1015
TaiI ACGT 1 cut(s) 546
TaqI TCGA 1 cut(s) 215
TasI AATT 4 cut(s) 309, 714, 957, 994
TatI WGTACW 1 cut(s) 747
TfiI GAWTC 2 cut(s) 669, 683
Tru1I TTAA 5 cut(s) 380, 503, 566, 600, 609
Tru9I TTAA 5 cut(s) 380, 503, 566, 600, 609
TseFI GTSAC 3 cut(s) 143, 400, 457
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 3 cut(s) 143, 400, 457
TspDTI ATGAA 5 cut(s) 164, 477, 635, 723, 750
VpaK11BI GGWCC 3 cut(s) 48, 299, 346
XagI CCTNNNNNAGG 1 cut(s) 770
XapI RAATTY 1 cut(s) 994
XspI CTAG 2 cut(s) 17, 1037
Zsp2I ATGCAT 1 cut(s) 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.