Rh2BG225200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004254
Physical Location & Seq
Forward (+)
21828039 .. 21833389
5351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG225200.1

Sequence Viewer

Length: 411 bp
ATGCCATACCGAAATTTTACGGAGAAAGATTGGAACTTTTCAGCAAGAGGAGCGACGTCCAGGGAAGAACAGCTACTCGTAGTTGCGGAGGAAGAAGAACTTTGCAGGAGGAAGAAGAAATTAAAAGTGCAGCAAGGGACAGAAAAGCACAGGGCACGAGCTGAATTCTTAAGGTCGCAATCCAAGTTCACAACAGTTGCTGCTACTATAGGAGGAAGAGAAGAGCAAGTATATGTGCCAGATGAAATGACTGCATTCTCAAGCAAGCTCGAGTACATCCCTGCAAGATGCCATCTTAGTGATCATGAGCATATATTTGGCACAGCCTTGCCACCAATTTCACAGATTGGAAACAAAGTTATCCAACAGAGACAAAAGGTCATAATCTTGTATGACAAGAAAGACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.78

Weight (kDa)

8.89

Isoelectric Point (pI)

58.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 59
AciI CCGC 1 cut(s) 86
AcsI RAATTY 2 cut(s) 13, 164
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 1 cut(s) 275
AflII CTTAAG 1 cut(s) 169
AhdI GACNNNNNGTC 1 cut(s) 377
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 3 cut(s) 73, 161, 268
AluI AGCT 3 cut(s) 73, 161, 268
Alw26I GTCTC 1 cut(s) 364
AlwNI CAGNNNCTG 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 269
ApeKI GCWGC 2 cut(s) 130, 200
ApoI RAATTY 2 cut(s) 13, 164
AvaI CYCGRG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 157
BarI GAAGNNNNNNTAC 2 cut(s) 57, 89
BauI CACGAG 1 cut(s) 156
BbvI GCAGC 2 cut(s) 142, 187
BccI CCATC 1 cut(s) 300
BciT130I CCWGG 1 cut(s) 61
BclI TGATCA 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 364
BfmI CTRYAG 1 cut(s) 207
BfrI CTTAAG 1 cut(s) 169
BisI GCNGC 2 cut(s) 131, 201
BlsI GCNGC 2 cut(s) 132, 202
Bme1390I CCNGG 1 cut(s) 61
BmeRI GACNNNNNGTC 1 cut(s) 377
BmeT110I CYCGRG 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 60
BseGI GGATG 1 cut(s) 276
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 2 cut(s) 142, 187
BsgI GTGCAG 1 cut(s) 149
BsiHKCI CYCGRG 1 cut(s) 269
BslFI GGGAC 1 cut(s) 151
BsmAI GTCTC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 1 cut(s) 301
BspACI CCGC 1 cut(s) 86
BspHI TCATGA 1 cut(s) 304
BspQI GCTCTTC 1 cut(s) 216
BspTI CTTAAG 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 1 cut(s) 301
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 2 cut(s) 211, 216
BstACI GRCGYC 1 cut(s) 56
BstAFI CTTAAG 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 276
BstKTI GATC 1 cut(s) 304
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 1 cut(s) 301
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 207
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 142, 187
BtsCI GGATG 1 cut(s) 276
Cac8I GCNNGC 1 cut(s) 266
CaiI CAGNNNCTG 1 cut(s) 200
CciI TCATGA 1 cut(s) 304
Csp6I GTAC 1 cut(s) 274
CviAII CATG 1 cut(s) 305
CviJI RGCY 4 cut(s) 73, 161, 268, 326
CviKI_1 RGCY 4 cut(s) 73, 161, 268, 326
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 296
DpnI GATC 1 cut(s) 303
DpnII GATC 1 cut(s) 301
DriI GACNNNNNGTC 1 cut(s) 377
Eam1104I CTCTTC 2 cut(s) 211, 216
Eam1105I GACNNNNNGTC 1 cut(s) 377
EarI CTCTTC 2 cut(s) 211, 216
Eco88I CYCGRG 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 59
FaeI CATG 1 cut(s) 308
FaiI YATR 9 cut(s) 7, 209, 232, 234, 306, 312, 314, 383, 393
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 1 cut(s) 151
FatI CATG 1 cut(s) 304
FbaI TGATCA 1 cut(s) 301
Fnu4HI GCNGC 2 cut(s) 131, 201
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 131, 201
GluI GCNGC 2 cut(s) 131, 201
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 189
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 1 cut(s) 189
Hpy99I CGWCG 1 cut(s) 58
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 4 cut(s) 105, 130, 254, 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 56
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 1 cut(s) 308
Ksp22I TGATCA 1 cut(s) 301
Kzo9I GATC 1 cut(s) 301
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 1 cut(s) 50
LpnPI CCDG 6 cut(s) 46, 73, 91, 136, 252, 294
Lsp1109I GCAGC 2 cut(s) 142, 187
LweI GCATC 1 cut(s) 278
MaeII ACGT 1 cut(s) 56
MalI GATC 1 cut(s) 303
MboI GATC 1 cut(s) 301
MboII GAAGA 7 cut(s) 77, 104, 107, 124, 127, 228, 233
MhlI GDGCHC 1 cut(s) 157
MluCI AATT 4 cut(s) 13, 119, 164, 336
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 4 cut(s) 41, 82, 102, 206
MseI TTAA 2 cut(s) 122, 170
MslI CAYNNNNRTG 1 cut(s) 297
MspCI CTTAAG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 1 cut(s) 254
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 1 cut(s) 301
NlaIII CATG 1 cut(s) 308
PaeR7I CTCGAG 1 cut(s) 269
PagI TCATGA 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 216
PctI GAATGC 1 cut(s) 254
PkrI GCNGC 2 cut(s) 132, 202
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspXI VCTCGAGB 1 cut(s) 269
PstNI CAGNNNCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 297
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 2 cut(s) 122, 170
SatI GCNGC 2 cut(s) 131, 201
Sau3AI GATC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 1 cut(s) 157
SetI ASST 6 cut(s) 59, 75, 163, 176, 270, 381
SfaNI GCATC 1 cut(s) 278
SfcI CTRYAG 1 cut(s) 207
Sfr274I CTCGAG 1 cut(s) 269
SlaI CTCGAG 1 cut(s) 269
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 3 cut(s) 169, 259, 269
SmoI CTYRAG 3 cut(s) 169, 259, 269
Sse9I AATT 4 cut(s) 13, 119, 164, 336
SsiI CCGC 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 59
TaaI ACNGT 1 cut(s) 196
TaiI ACGT 1 cut(s) 59
TaqI TCGA 1 cut(s) 270
TasI AATT 4 cut(s) 13, 119, 164, 336
TatI WGTACW 1 cut(s) 273
Tru1I TTAA 2 cut(s) 122, 170
Tru9I TTAA 2 cut(s) 122, 170
TseI GCWGC 2 cut(s) 130, 200
TspDTI ATGAA 1 cut(s) 258
TspGWI ACGGA 1 cut(s) 35
Vha464I CTTAAG 1 cut(s) 169
XapI RAATTY 2 cut(s) 13, 164
XhoI CTCGAG 1 cut(s) 269
ZraI GACGTC 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.