Rh2DG183200

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006508
Physical Location & Seq
Reverse (-)
16184372 .. 16192217
7846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG183200.1

Sequence Viewer

Length: 906 bp
ATGGTGAGCGCAAGTGCAATGCGTGCTGTACAACCCGCAATCCTCTCCTCCTTTGTGGGTACCCACCTGCTTCACTCACCGCAGCCTCTTCACCAACTCTTCCTCTACAACCCAGGAAGCAAGCATGTCACTATGCAGTTATCCAGAGGATTTTCGGGCCTGACCGACCTCTTGTTCAATGGAAGAAATGCAGGTGAAGTGCTAAATGCCAAGCGGAAACGCTTAAGGCCTGGAAAAGTTTCACCTCGTCGCCCTGTTCCAGATCATATACCAAGGCCGCCTTATGTAAAATCTAAAAAACCGCCTGGAATTGCGAGTGGACCTGAAGTGCATGATCATGAGGGAATAGAATGCATGAGAGCTTCTGGGAGGCTTGCAGCACAGGTTCTTGAATATGCTGGGACTTTGGTCAAGCCAGGCATAAAGACTGATGAAATTGACCAAGCTGTTCACCAAATGATAATTGATAATGGGGCCTACCCTTCACCACTTGGCTATGGTGGATTTCCAAAGAGCGTCTGCACATCAGTGAATGAATGCATTTGCCATGGAATACCAGATTCCCGAGAACTGGAGGATGGTGACATAGTTAACATTGATGTCACAGTCTATTTGAATGGGTACCATGGCGATACATCAAAAACTTTCTTTTGTGGAGATGTTGATGATGCAGGCAGAAACTTAGTTCAGGTAACTAAGGAATGCCTTGACAAAGCAATATCAATTTGTGCACCTGGCGTGGAGTTCAAGAAAATTGGCAGAACAATCCAAGAGCATGCAGATAAACATCGTTATGGTGTTGTTAGACAGTTCGTTGGGCATGGTGTTGGACGCGTCTTTCATGCTGATCCAGTTGTTTTGCACTACAGTAATTATTCTGAACTTTATGCTGCTCCTGCCTTCTAG

Protein Analysis

301

Amino Acids

32.84

Weight (kDa)

8.41

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 117 - 288 6.1e-45 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 75, 182
Acc36I ACCTGC 2 cut(s) 75, 182
Acc65I GGTACC 2 cut(s) 59, 621
AccB1I GGYRCC 2 cut(s) 59, 621
AccII CGCG 1 cut(s) 834
AciI CCGC 5 cut(s) 36, 80, 214, 278, 302
AclWI GGATC 1 cut(s) 842
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 3 cut(s) 30, 61, 623
AfiI CCNNNNNNNGG 1 cut(s) 571
AflII CTTAAG 1 cut(s) 223
AflIII ACRYGT 1 cut(s) 832
AgsI TTSAA 4 cut(s) 178, 392, 616, 748
AjnI CCWGG 5 cut(s) 112, 229, 304, 415, 733
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 2 cut(s) 362, 446
AluI AGCT 2 cut(s) 362, 446
Alw21I GWGCWC 1 cut(s) 733
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 1 cut(s) 842
Ama87I CYCGRG 1 cut(s) 564
AoxI GGCC 4 cut(s) 157, 227, 275, 474
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 3 cut(s) 82, 377, 890
Asp700I GAANNNNTTC 1 cut(s) 238
Asp718I GGTACC 2 cut(s) 59, 621
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 3 cut(s) 157, 320, 474
AsuHPI GGTGA 8 cut(s) 16, 69, 83, 206, 234, 443, 477, 593
AvaI CYCGRG 1 cut(s) 564
AvaII GGWCC 1 cut(s) 320
BaeGI GKGCMC 1 cut(s) 733
BanI GGYRCC 2 cut(s) 59, 621
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 3 cut(s) 94, 389, 877
BccI CCATC 1 cut(s) 572
BciT130I CCWGG 5 cut(s) 114, 231, 306, 417, 735
BclI TGATCA 1 cut(s) 334
BfaI CTAG 1 cut(s) 904
BfmI CTRYAG 1 cut(s) 865
BfrI CTTAAG 1 cut(s) 223
BfuAI ACCTGC 2 cut(s) 75, 182
BisI GCNGC 4 cut(s) 83, 278, 378, 891
BlsI GCNGC 4 cut(s) 84, 279, 379, 892
Bme1390I CCNGG 5 cut(s) 114, 231, 306, 417, 735
Bme18I GGWCC 1 cut(s) 320
BmeT110I CYCGRG 1 cut(s) 564
BmgT120I GGNCC 3 cut(s) 157, 320, 474
BmiI GGNNCC 3 cut(s) 61, 475, 623
BmrFI CCNGG 5 cut(s) 114, 231, 306, 417, 735
BmsI GCATC 1 cut(s) 658
BoxI GACNNNNGTC 1 cut(s) 407
BpmI CTGGAG 1 cut(s) 593
BsaBI GATNNNNATC 1 cut(s) 786
BsaJI CCNNGG 4 cut(s) 112, 272, 547, 625
Bsc4I CCNNNNNNNGG 1 cut(s) 571
Bse1I ACTGG 2 cut(s) 576, 851
Bse3DI GCAATG 1 cut(s) 24
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 5 cut(s) 114, 231, 306, 417, 735
BseDI CCNNGG 4 cut(s) 112, 272, 547, 625
BseGI GGATG 1 cut(s) 583
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 1 cut(s) 571
BseMI GCAATG 1 cut(s) 24
BseNI ACTGG 2 cut(s) 576, 851
BseRI GAGGAG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 3 cut(s) 94, 389, 877
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 505
Bsh1236I CGCG 1 cut(s) 834
BshFI GGCC 4 cut(s) 159, 229, 277, 476
BshNI GGYRCC 2 cut(s) 59, 621
BsiHKAI GWGCWC 1 cut(s) 733
BsiHKCI CYCGRG 1 cut(s) 564
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 1 cut(s) 571
BsmFI GGGAC 1 cut(s) 415
BsmI GAATGC 3 cut(s) 356, 542, 707
BsnI GGCC 4 cut(s) 159, 229, 277, 476
BsoBI CYCGRG 1 cut(s) 564
Bsp1286I GDGCHC 1 cut(s) 733
Bsp1407I TGTACA 1 cut(s) 28
Bsp143I GATC 3 cut(s) 262, 334, 847
Bsp19I CCATGG 2 cut(s) 547, 625
BspACI CCGC 5 cut(s) 36, 80, 214, 278, 302
BspANI GGCC 4 cut(s) 159, 229, 277, 476
BspFNI CGCG 1 cut(s) 834
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 3 cut(s) 61, 475, 623
BspMI ACCTGC 2 cut(s) 75, 182
BspPI GGATC 1 cut(s) 842
BspT107I GGYRCC 2 cut(s) 59, 621
BspTI CTTAAG 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 28
BsrI ACTGG 2 cut(s) 576, 851
BssECI CCNNGG 4 cut(s) 112, 272, 547, 625
BssMI GATC 3 cut(s) 262, 334, 847
BssT1I CCWWGG 3 cut(s) 272, 547, 625
Bst2UI CCWGG 5 cut(s) 114, 231, 306, 417, 735
Bst4CI ACNGT 3 cut(s) 607, 810, 869
Bst6I CTCTTC 2 cut(s) 93, 104
BstAFI CTTAAG 1 cut(s) 223
BstAPI GCANNNNNTGC 1 cut(s) 23
BstAUI TGTACA 1 cut(s) 28
BstC8I GCNNGC 5 cut(s) 24, 122, 375, 673, 777
BstDEI CTNAG 2 cut(s) 682, 696
BstDSI CCRYGG 2 cut(s) 547, 625
BstF5I GGATG 1 cut(s) 583
BstFNI CGCG 1 cut(s) 834
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 3 cut(s) 265, 337, 850
BstMBI GATC 3 cut(s) 262, 334, 847
BstMWI GCNNNNNNNGC 2 cut(s) 23, 896
BstNI CCWGG 5 cut(s) 114, 231, 306, 417, 735
BstNSI RCATGY 2 cut(s) 128, 779
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 5 cut(s) 112, 229, 304, 415, 733
BstSFI CTRYAG 1 cut(s) 865
BstSLI GKGCMC 1 cut(s) 733
BstUI CGCG 1 cut(s) 834
BstV1I GCAGC 3 cut(s) 94, 389, 877
BsuRI GGCC 4 cut(s) 159, 229, 277, 476
BtgI CCRYGG 2 cut(s) 547, 625
BtsCI GGATG 1 cut(s) 583
BtsIMutI CAGTG 1 cut(s) 534
BveI ACCTGC 2 cut(s) 75, 182
Cac8I GCNNGC 5 cut(s) 24, 122, 375, 673, 777
CciI TCATGA 1 cut(s) 337
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 3 cut(s) 157, 320, 474
CseI GACGC 3 cut(s) 505, 823, 840
Csp6I GTAC 3 cut(s) 29, 60, 622
CviAII CATG 9 cut(s) 125, 332, 338, 355, 548, 626, 776, 821, 842
CviQI GTAC 3 cut(s) 29, 60, 622
DdeI CTNAG 2 cut(s) 682, 696
DpnI GATC 3 cut(s) 264, 336, 849
DpnII GATC 3 cut(s) 262, 334, 847
Eam1104I CTCTTC 2 cut(s) 93, 104
EarI CTCTTC 2 cut(s) 93, 104
Eco130I CCWWGG 3 cut(s) 272, 547, 625
Eco147I AGGCCT 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 564
EcoO109I RGGNCCY 1 cut(s) 474
EcoRII CCWGG 5 cut(s) 112, 229, 304, 415, 733
EcoT14I CCWWGG 3 cut(s) 272, 547, 625
EcoT22I ATGCAT 2 cut(s) 356, 542
ErhI CCWWGG 3 cut(s) 272, 547, 625
FaeI CATG 9 cut(s) 128, 335, 341, 358, 551, 629, 779, 824, 845
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FaqI GGGAC 1 cut(s) 415
FatI CATG 9 cut(s) 124, 331, 337, 354, 547, 625, 775, 820, 841
FauI CCCGC 1 cut(s) 43
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 4 cut(s) 83, 278, 378, 891
FokI GGATG 1 cut(s) 590
Fsp4HI GCNGC 4 cut(s) 83, 278, 378, 891
FspBI CTAG 1 cut(s) 904
GlaI GCGC 1 cut(s) 10
GluI GCNGC 4 cut(s) 83, 278, 378, 891
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 593
HaeIII GGCC 4 cut(s) 159, 229, 277, 476
HgaI GACGC 3 cut(s) 505, 823, 840
HhaI GCGC 1 cut(s) 11
Hin1II CATG 9 cut(s) 128, 335, 341, 358, 551, 629, 779, 824, 845
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HinfI GANTC 1 cut(s) 560
HpaI GTTAAC 1 cut(s) 592
HphI GGTGA 8 cut(s) 16, 69, 83, 206, 234, 443, 477, 593
Hpy166II GTNNAC 4 cut(s) 320, 451, 592, 731
Hpy188I TCNGA 1 cut(s) 880
Hpy188III TCNNGA 6 cut(s) 144, 260, 338, 389, 564, 748
Hpy8I GTNNAC 4 cut(s) 320, 451, 592, 731
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 1 cut(s) 492
HpyCH4III ACNGT 3 cut(s) 607, 810, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 896
HpyF3I CTNAG 2 cut(s) 682, 696
Hsp92II CATG 9 cut(s) 128, 335, 341, 358, 551, 629, 779, 824, 845
HspAI GCGC 1 cut(s) 9
KpnI GGTACC 2 cut(s) 63, 625
Ksp22I TGATCA 1 cut(s) 334
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 3 cut(s) 262, 334, 847
LmnI GCTCC 1 cut(s) 898
Lsp1109I GCAGC 3 cut(s) 94, 389, 877
LweI GCATC 1 cut(s) 658
MaeI CTAG 1 cut(s) 904
MaeIII GTNAC 4 cut(s) 127, 581, 601, 691
MalI GATC 3 cut(s) 264, 336, 849
MboI GATC 3 cut(s) 262, 334, 847
MboII GAAGA 3 cut(s) 80, 91, 195
MhlI GDGCHC 1 cut(s) 733
MluCI AATT 6 cut(s) 309, 435, 462, 723, 753, 871
MluI ACGCGT 1 cut(s) 832
MmeI TCCRAC 1 cut(s) 808
Mph1103I ATGCAT 2 cut(s) 356, 542
MroXI GAANNNNTTC 1 cut(s) 238
MseI TTAA 2 cut(s) 224, 591
MslI CAYNNNNRTG 3 cut(s) 336, 527, 792
MspCI CTTAAG 1 cut(s) 223
MspR9I CCNGG 5 cut(s) 114, 231, 306, 417, 735
Mva1269I GAATGC 3 cut(s) 356, 542, 707
MvaI CCWGG 5 cut(s) 114, 231, 306, 417, 735
MvnI CGCG 1 cut(s) 834
MwoI GCNNNNNNNGC 2 cut(s) 23, 896
NcoI CCATGG 2 cut(s) 547, 625
NdeII GATC 3 cut(s) 262, 334, 847
NlaIII CATG 9 cut(s) 128, 335, 341, 358, 551, 629, 779, 824, 845
NlaIV GGNNCC 3 cut(s) 61, 475, 623
NmuCI GTSAC 3 cut(s) 127, 581, 601
NsiI ATGCAT 2 cut(s) 356, 542
NspI RCATGY 2 cut(s) 128, 779
OliI CACNNNNGTG 1 cut(s) 527
PaeI GCATGC 1 cut(s) 779
PagI TCATGA 1 cut(s) 337
PaqCI CACCTGC 2 cut(s) 75, 182
PceI AGGCCT 1 cut(s) 229
PctI GAATGC 3 cut(s) 356, 542, 707
PdmI GAANNNNTTC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 560
PkrI GCNGC 4 cut(s) 84, 279, 379, 892
PshAI GACNNNNGTC 1 cut(s) 407
Psp6I CCWGG 5 cut(s) 112, 229, 304, 415, 733
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 5 cut(s) 112, 229, 304, 415, 733
PspN4I GGNNCC 3 cut(s) 61, 475, 623
PspPI GGNCC 3 cut(s) 157, 320, 474
RsaI GTAC 3 cut(s) 30, 61, 623
RsaNI GTAC 3 cut(s) 29, 60, 622
RseI CAYNNNNRTG 3 cut(s) 336, 527, 792
SaqAI TTAA 2 cut(s) 224, 591
SatI GCNGC 4 cut(s) 83, 278, 378, 891
Sau3AI GATC 3 cut(s) 262, 334, 847
Sau96I GGNCC 3 cut(s) 157, 320, 474
ScrFI CCNGG 5 cut(s) 114, 231, 306, 417, 735
SduI GDGCHC 1 cut(s) 733
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 1 cut(s) 865
SinI GGWCC 1 cut(s) 320
SmiMI CAYNNNNRTG 3 cut(s) 336, 527, 792
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
SphI GCATGC 1 cut(s) 779
Sse9I AATT 6 cut(s) 309, 435, 462, 723, 753, 871
SseBI AGGCCT 1 cut(s) 229
SsiI CCGC 5 cut(s) 36, 80, 214, 278, 302
SspMI CTAG 1 cut(s) 904
StuI AGGCCT 1 cut(s) 229
StyD4I CCNGG 5 cut(s) 112, 229, 304, 415, 733
StyI CCWWGG 3 cut(s) 272, 547, 625
TaaI ACNGT 3 cut(s) 607, 810, 869
TaqII GACCGA 1 cut(s) 179
TasI AATT 6 cut(s) 309, 435, 462, 723, 753, 871
TatI WGTACW 1 cut(s) 28
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 1 cut(s) 560
Tru1I TTAA 2 cut(s) 224, 591
Tru9I TTAA 2 cut(s) 224, 591
TscAI CASTG 1 cut(s) 534
TseFI GTSAC 3 cut(s) 127, 581, 601
TseI GCWGC 3 cut(s) 82, 377, 890
Tsp45I GTSAC 3 cut(s) 127, 581, 601
TspDTI ATGAA 3 cut(s) 447, 549, 830
TspRI CASTG 1 cut(s) 534
Vha464I CTTAAG 1 cut(s) 223
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 1 cut(s) 320
XceI RCATGY 2 cut(s) 128, 779
XmnI GAANNNNTTC 1 cut(s) 238
XspI CTAG 1 cut(s) 904
Zsp2I ATGCAT 2 cut(s) 356, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.