Rh2DG655400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011612
Physical Location & Seq
Forward (+)
88053712 .. 88054380
669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG655400.1

Sequence Viewer

Length: 441 bp
ATGTTTGTCAAAGATTTAGAGTTAGGTGCTGAAATTTTCGCTGGAGGTGCAGTATCACTGGTTGTCAACGATAGATTCTGGAGTCCTCATGCATTCCAGGTTAAGTTGAATTGCAGCTCCTTTTGGGTCAAGTCAAGTTGTAAGGTTGGATTATGTGTCATTGCATGCGATGCTGATCGTTCTCTAGTCTTTTCTGAATGTGTGCTTGGGGTGGCCATATCGGCACCTGATGCAGAAGCAAAAGCGTGCCTCATGGCTTTACGACTTGCCACAGAACAACTTGCAGGCCGACCATTTGTGCTTGAGTCTCGTTTTTTCCGAACGCCCTTCACGACATTCGAAGATTTTGGCACATCCTTGGCACCGTCGGTGGCTAGGTTAATCACCCGGTACCACCAGGAGAATATTCATTGGAGTAAGTGCGAAAGGTTTGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

16.25

Weight (kDa)

6.28

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 390
AccB1I GGYRCC 3 cut(s) 223, 361, 390
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 1 cut(s) 33
AfaI GTAC 1 cut(s) 392
AgsI TTSAA 1 cut(s) 109
AjnI CCWGG 2 cut(s) 96, 396
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 1 cut(s) 117
AluI AGCT 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 312
AoxI GGCC 2 cut(s) 213, 286
ApeKI GCWGC 1 cut(s) 114
ApoI RAATTY 1 cut(s) 33
Asp718I GGTACC 1 cut(s) 390
AsuC2I CCSGG 1 cut(s) 388
AsuHPI GGTGA 1 cut(s) 376
AsuII TTCGAA 1 cut(s) 339
BalI TGGCCA 1 cut(s) 215
BanI GGYRCC 3 cut(s) 223, 361, 390
BbvI GCAGC 1 cut(s) 126
BciT130I CCWGG 2 cut(s) 98, 398
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 2 cut(s) 185, 375
BglI GCCNNNNNGGC 1 cut(s) 221
BisI GCNGC 1 cut(s) 115
BlsI GCNGC 1 cut(s) 116
Bme1390I CCNGG 3 cut(s) 98, 388, 398
BmiI GGNNCC 3 cut(s) 225, 363, 392
BmrFI CCNGG 3 cut(s) 98, 388, 398
BmsI GCATC 2 cut(s) 160, 220
BpmI CTGGAG 2 cut(s) 63, 100
Bpu14I TTCGAA 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 1 cut(s) 388
BsaBI GATNNNNATC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bse1I ACTGG 1 cut(s) 63
Bse3DI GCAATG 1 cut(s) 159
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 2 cut(s) 98, 398
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 353
BseJI GATNNNNATC 1 cut(s) 174
BseMI GCAATG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 63
BseXI GCAGC 1 cut(s) 126
BsgI GTGCAG 1 cut(s) 69
BshFI GGCC 2 cut(s) 215, 288
BshNI GGYRCC 3 cut(s) 223, 361, 390
BsiSI CCGG 1 cut(s) 388
BsmAI GTCTC 1 cut(s) 312
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 2 cut(s) 215, 288
Bsp119I TTCGAA 1 cut(s) 339
Bsp143I GATC 1 cut(s) 175
BspANI GGCC 2 cut(s) 215, 288
BspLI GGNNCC 3 cut(s) 225, 363, 392
BspT104I TTCGAA 1 cut(s) 339
BspT107I GGYRCC 3 cut(s) 223, 361, 390
BsrDI GCAATG 1 cut(s) 159
BsrI ACTGG 1 cut(s) 63
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 2 cut(s) 98, 398
Bst4CI ACNGT 1 cut(s) 366
BstAPI GCANNNNNTGC 2 cut(s) 170, 230
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 3 cut(s) 166, 247, 286
BstF5I GGATG 1 cut(s) 353
BstKTI GATC 1 cut(s) 178
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 4 cut(s) 47, 170, 221, 230
BstNI CCWGG 2 cut(s) 98, 398
BstNSI RCATGY 1 cut(s) 168
BstSCI CCNGG 3 cut(s) 96, 386, 396
BstV1I GCAGC 1 cut(s) 126
BsuRI GGCC 2 cut(s) 215, 288
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 3 cut(s) 166, 247, 286
Csp6I GTAC 1 cut(s) 391
CviAII CATG 3 cut(s) 89, 165, 253
CviJI RGCY 5 cut(s) 117, 215, 257, 288, 374
CviKI_1 RGCY 5 cut(s) 117, 215, 257, 288, 374
CviQI GTAC 1 cut(s) 391
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 96, 396
EcoT14I CCWWGG 1 cut(s) 357
EcoT22I ATGCAT 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 3 cut(s) 92, 168, 256
FaiI YATR 5 cut(s) 90, 154, 166, 218, 254
FatI CATG 3 cut(s) 88, 164, 252
Fnu4HI GCNGC 1 cut(s) 115
FokI GGATG 1 cut(s) 340
Fsp4HI GCNGC 1 cut(s) 115
FspBI CTAG 2 cut(s) 185, 375
GluI GCNGC 1 cut(s) 115
GsuI CTGGAG 2 cut(s) 63, 100
HaeIII GGCC 2 cut(s) 215, 288
HapII CCGG 1 cut(s) 388
Hin1II CATG 3 cut(s) 92, 168, 256
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 3 cut(s) 75, 82, 305
HpaII CCGG 1 cut(s) 388
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 196, 320
Hpy188III TCNNGA 2 cut(s) 79, 331
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4V TGCA 6 cut(s) 50, 92, 114, 164, 233, 284
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 170, 221, 230
Hsp92II CATG 3 cut(s) 92, 168, 256
KpnI GGTACC 1 cut(s) 394
Kzo9I GATC 1 cut(s) 175
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 2 cut(s) 160, 220
MaeI CTAG 2 cut(s) 185, 375
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 3 cut(s) 33, 109, 436
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 2 cut(s) 91, 314
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 3 cut(s) 38, 96, 260
Mox20I TGGCCA 1 cut(s) 215
Mph1103I ATGCAT 1 cut(s) 94
MscI TGGCCA 1 cut(s) 215
MseI TTAA 2 cut(s) 102, 380
Msp20I TGGCCA 1 cut(s) 215
MspI CCGG 1 cut(s) 388
MspR9I CCNGG 3 cut(s) 98, 388, 398
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 2 cut(s) 98, 398
MwoI GCNNNNNNNGC 4 cut(s) 47, 170, 221, 230
NciI CCSGG 1 cut(s) 388
NdeII GATC 1 cut(s) 175
NlaIII CATG 3 cut(s) 92, 168, 256
NlaIV GGNNCC 3 cut(s) 225, 363, 392
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 1 cut(s) 168
NspV TTCGAA 1 cut(s) 339
PaeI GCATGC 1 cut(s) 168
PcsI WCGNNNNNNNCGW 2 cut(s) 316, 329
PctI GAATGC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 75
PkrI GCNGC 1 cut(s) 116
PleI GAGTC 2 cut(s) 90, 313
PpsI GAGTC 2 cut(s) 90, 313
Psp6I CCWGG 2 cut(s) 96, 396
PspGI CCWGG 2 cut(s) 96, 396
PspN4I GGNNCC 3 cut(s) 225, 363, 392
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SaqAI TTAA 2 cut(s) 102, 380
SatI GCNGC 1 cut(s) 115
Sau3AI GATC 1 cut(s) 175
SchI GAGTC 2 cut(s) 91, 314
ScrFI CCNGG 3 cut(s) 98, 388, 398
SetI ASST 8 cut(s) 28, 49, 102, 119, 147, 229, 380, 431
SfaNI GCATC 2 cut(s) 160, 220
SfuI TTCGAA 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SphI GCATGC 1 cut(s) 168
Sse9I AATT 3 cut(s) 33, 109, 436
SspI AATATT 1 cut(s) 406
SspMI CTAG 2 cut(s) 185, 375
StyD4I CCNGG 3 cut(s) 96, 386, 396
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 1 cut(s) 366
TaqI TCGA 1 cut(s) 339
TasI AATT 3 cut(s) 33, 109, 436
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 2 cut(s) 102, 380
Tru9I TTAA 2 cut(s) 102, 380
TscAI CASTG 1 cut(s) 63
TseI GCWGC 1 cut(s) 114
TspDTI ATGAA 1 cut(s) 398
TspRI CASTG 1 cut(s) 63
XapI RAATTY 1 cut(s) 33
XceI RCATGY 1 cut(s) 168
XspI CTAG 2 cut(s) 185, 375
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.