Rh3AG016900

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0004276
Physical Location & Seq
Reverse (-)
1075842 .. 1078201
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG016900.1

Sequence Viewer

Length: 390 bp
ATGGGGAACGATACAGAGTTGGTGACCCCAGGTGAAAAGCTTGGAAGAGCCTCCGAATTCAAAGCAGGCAGAGGTGCCTACGTGTCACCTGATAATCTCACCGTCTACGCTTCCCTCACTGGCCTCCGCCGCATCCTATCCCCGCCTCCTGACTCACCTGACCAGAGACCCATTGTGGAAGTAACTGGTCACAAGGCCCATGGTGCTATTCCGGAGCCTGGATCTATTGTCATTGCCAGAGTTACCAAAGTGATGGCCAGAATGGCTTCTGCTGATATCATGTGTCTAGGCACCAAGTCTGTGCGGGAAAACTTTACCGGCATTATTAGGTGTGCCAATGGTGGCAATAAGTTGGACAGAGATGCAATGCCCCATCACAGGGCAAATTGA

Protein Analysis

129

Amino Acids

13.77

Weight (kDa)

9.39

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECR1_N PF14382 7 - 45 5.3e-10 Exosome complex exonuclease RRP4 N-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 74, 290
AccI GTMKAC 1 cut(s) 105
AccIII TCCGGA 1 cut(s) 211
AciI CCGC 4 cut(s) 127, 130, 143, 304
AclWI GGATC 1 cut(s) 229
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 56
AfiI CCNNNNNNNGG 3 cut(s) 218, 378, 379
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 1 cut(s) 61
AjnI CCWGG 2 cut(s) 28, 217
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 1 cut(s) 229
Aor13HI TCCGGA 1 cut(s) 211
AoxI GGCC 3 cut(s) 121, 195, 255
ApoI RAATTY 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 265
AspS9I GGNCC 1 cut(s) 196
AsuHPI GGTGA 5 cut(s) 34, 44, 78, 91, 147
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 2 cut(s) 74, 290
BccI CCATC 2 cut(s) 247, 381
BciT130I CCWGG 2 cut(s) 30, 219
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 287
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 1 cut(s) 130
BlsI GCNGC 1 cut(s) 131
Bme1390I CCNGG 2 cut(s) 30, 219
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 3 cut(s) 76, 216, 292
BmrFI CCNGG 2 cut(s) 30, 219
BmsI GCATC 2 cut(s) 141, 352
BsaAI YACGTR 1 cut(s) 82
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 2 cut(s) 28, 199
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 378, 379
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 2 cut(s) 124, 190
Bse3DI GCAATG 2 cut(s) 231, 372
BseAI TCCGGA 1 cut(s) 211
BseBI CCWGG 2 cut(s) 30, 219
BseDI CCNNGG 2 cut(s) 28, 199
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 3 cut(s) 218, 378, 379
BseMI GCAATG 2 cut(s) 231, 372
BseNI ACTGG 2 cut(s) 124, 190
BshFI GGCC 3 cut(s) 123, 197, 257
BshNI GGYRCC 2 cut(s) 74, 290
BsiSI CCGG 2 cut(s) 212, 318
BslI CCNNNNNNNGG 3 cut(s) 218, 378, 379
BsmAI GTCTC 1 cut(s) 160
BsnI GGCC 3 cut(s) 123, 197, 257
Bso31I GGTCTC 1 cut(s) 160
Bsp13I TCCGGA 1 cut(s) 211
Bsp143I GATC 1 cut(s) 221
Bsp19I CCATGG 1 cut(s) 199
BspACI CCGC 4 cut(s) 127, 130, 143, 304
BspANI GGCC 3 cut(s) 123, 197, 257
BspEI TCCGGA 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 76, 216, 292
BspPI GGATC 1 cut(s) 229
BspQI GCTCTTC 1 cut(s) 40
BspT107I GGYRCC 2 cut(s) 74, 290
BspTNI GGTCTC 1 cut(s) 160
BsrDI GCAATG 2 cut(s) 231, 372
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 2 cut(s) 124, 190
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 28, 199
BssMI GATC 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 199
Bst2UI CCWGG 2 cut(s) 30, 219
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 1 cut(s) 40
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 199
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 1 cut(s) 132
BstKTI GATC 1 cut(s) 224
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 1 cut(s) 221
BstMWI GCNNNNNNNGC 3 cut(s) 129, 203, 263
BstNI CCWGG 2 cut(s) 30, 219
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 28, 217
BstX2I RGATCY 1 cut(s) 221
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 1 cut(s) 221
BsuRI GGCC 3 cut(s) 123, 197, 257
BtgI CCRYGG 1 cut(s) 199
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 196
CviAII CATG 2 cut(s) 200, 280
CviJI RGCY 7 cut(s) 40, 50, 123, 197, 217, 257, 266
CviKI_1 RGCY 7 cut(s) 40, 50, 123, 197, 217, 257, 266
DpnI GATC 1 cut(s) 223
DpnII GATC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
EciI GGCGGA 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 160
Eco32I GATATC 1 cut(s) 277
Eco91I GGTNACC 1 cut(s) 22
EcoO65I GGTNACC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 56
EcoRII CCWGG 2 cut(s) 28, 217
EcoRV GATATC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 199
FaeI CATG 2 cut(s) 203, 283
FaiI YATR 2 cut(s) 201, 281
FatI CATG 2 cut(s) 199, 279
FauI CCCGC 2 cut(s) 150, 297
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 1 cut(s) 130
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 1 cut(s) 130
FspBI CTAG 1 cut(s) 287
GluI GCNGC 1 cut(s) 130
HaeIII GGCC 3 cut(s) 123, 197, 257
HapII CCGG 2 cut(s) 212, 318
Hin1II CATG 2 cut(s) 203, 283
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 1 cut(s) 152
HpaII CCGG 2 cut(s) 212, 318
HphI GGTGA 5 cut(s) 34, 44, 78, 91, 147
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 2 cut(s) 149, 212
Hpy8I GTNNAC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 203, 263
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 2 cut(s) 203, 283
Kpn2I TCCGGA 1 cut(s) 211
Kzo9I GATC 1 cut(s) 221
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 1 cut(s) 214
LweI GCATC 2 cut(s) 141, 352
MaeI CTAG 1 cut(s) 287
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 5 cut(s) 22, 84, 181, 188, 241
MalI GATC 1 cut(s) 223
MboI GATC 1 cut(s) 221
MboII GAAGA 1 cut(s) 57
MflI RGATCY 1 cut(s) 221
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 2 cut(s) 56, 385
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 5 cut(s) 61, 65, 125, 134, 156
Mox20I TGGCCA 1 cut(s) 257
MroI TCCGGA 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 265
MscI TGGCCA 1 cut(s) 257
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 2 cut(s) 212, 318
MspR9I CCNGG 2 cut(s) 30, 219
MvaI CCWGG 2 cut(s) 30, 219
MwoI GCNNNNNNNGC 3 cut(s) 129, 203, 263
NcoI CCATGG 1 cut(s) 199
NdeII GATC 1 cut(s) 221
NlaIII CATG 2 cut(s) 203, 283
NlaIV GGNNCC 3 cut(s) 76, 216, 292
NmuCI GTSAC 3 cut(s) 22, 84, 188
PciSI GCTCTTC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 265
PkrI GCNGC 1 cut(s) 131
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Ppu21I YACGTR 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 28, 217
PspEI GGTNACC 1 cut(s) 22
PspGI CCWGG 2 cut(s) 28, 217
PspN4I GGNNCC 3 cut(s) 76, 216, 292
PspPI GGNCC 1 cut(s) 196
PsuI RGATCY 1 cut(s) 221
SapI GCTCTTC 1 cut(s) 40
SatI GCNGC 1 cut(s) 130
Sau3AI GATC 1 cut(s) 221
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 30, 219
SetI ASST 7 cut(s) 34, 42, 76, 84, 91, 160, 332
SfaNI GCATC 2 cut(s) 141, 352
Sse9I AATT 2 cut(s) 56, 385
SsiI CCGC 4 cut(s) 127, 130, 143, 304
SspMI CTAG 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 28, 217
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 1 cut(s) 84
TasI AATT 2 cut(s) 56, 385
TauI GCSGC 1 cut(s) 132
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 3 cut(s) 22, 84, 188
Tsp45I GTSAC 3 cut(s) 22, 84, 188
TspRI CASTG 1 cut(s) 124
XapI RAATTY 1 cut(s) 56
XmiI GTMKAC 1 cut(s) 105
XmnI GAANNNNTTC 1 cut(s) 265
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.