Rh3AG019400
MYB Family

PHR1-LIKE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0003102
Physical Location & Seq
Forward (+)
1240326 .. 1242035
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG019400.1

Sequence Viewer

Length: 918 bp
ATGAGTAACTTGGGGACTTCCAAAGCTATGTCTTCAGCATTTCCACATCTCCCTGCCCCTCTTGATGTCAATTATTCCAGATTGCCAGATTCTTTTCAGGTTTCCGCACAAAGAGAAAAGCCTATGCATTCAATGTTACCACAGTCCTCTTCCCTTGGGAACTCATTTCCATCATCGTCCAGGCTTCCCTATGACAGTCAGATTTCTTCAGTTTACCCTCATGAAAGACATTCCCAAATTTTTCCAATCAATAAGTTACCAAGTGGAGAATCACTGTCACTGACCCATTCACAGTTACAATCTACACCGGTGGCTAGTTACCCTGAGCAAACTAAAGATATTTCCTGGTGCCCAGATTCAATTCAGGAGTTTCTGCAGTTTCCTGAAAATCTCCCTGATCAGAATGGCCTGGTAGATACTAGTACTGGTGTCATAATGTCTGATGATCATGCTGAAAAGACTGATTGGTCTGACTTGTATCCATTAATTTCTTTTGATGGTCCTCTGGATCCATATTGGGAGCTTTCGATTGATGATATTCCTGCGGTGGATCCCAAACCGGAGGTGTTGAAGCATTCTTCTGATATCCCAGTGCAGCAGCCCAAGATTCAACAGCATCAGCCAGTACAATCTGGAGAAATTTTTACTAGCCCTGACCCTGTGTCCTCTGCACCTTCAACCAAAGCTAGAATGCGTTGGACACAAGAACTTCATGAGGCCTTTGTTGAAGCTGTCAACCAGCTTGGTGGTAGTGAAAGAGCCACTCCTAAGGGTATCTTAAACGTCATGAAGGTTGAAAGCTTGACAATCTGTCATGTAAAATGCCATTTACAGAAATATAGAACAGCCAGATACAAACCAGAGTCATCAGAAGGAACTTGTGAGAAGAATCTGACTCCTGTTGAAGAAATGAATTAG

Protein Analysis

305

Amino Acids

33.97

Weight (kDa)

5.17

Isoelectric Point (pI)

60.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 230 - 281 8.6e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 466
AccB1I GGYRCC 1 cut(s) 348
AciI CCGC 2 cut(s) 105, 545
AclWI GGATC 4 cut(s) 503, 516, 545, 558
AcsI RAATTY 2 cut(s) 237, 639
AcuI CTGAAG 2 cut(s) 18, 192
AfaI GTAC 2 cut(s) 424, 627
AgeI ACCGGT 1 cut(s) 307
AgsI TTSAA 8 cut(s) 132, 360, 571, 611, 678, 728, 797, 905
AhdI GACNNNNNGTC 2 cut(s) 661, 810
AhlI ACTAGT 1 cut(s) 419
AjnI CCWGG 3 cut(s) 179, 344, 408
AluBI AGCT 6 cut(s) 26, 523, 686, 731, 742, 801
AluI AGCT 6 cut(s) 26, 523, 686, 731, 742, 801
AlwI GGATC 4 cut(s) 503, 516, 545, 558
AoxI GGCC 2 cut(s) 406, 717
ApeKI GCWGC 2 cut(s) 595, 598
ApoI RAATTY 2 cut(s) 237, 639
AseI ATTAAT 1 cut(s) 485
AsiGI ACCGGT 1 cut(s) 307
AspS9I GGNCC 1 cut(s) 500
AvaII GGWCC 1 cut(s) 500
AxyI CCTNAGG 1 cut(s) 768
BaeGI GKGCMC 1 cut(s) 353
BamHI GGATCC 2 cut(s) 508, 550
BanI GGYRCC 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 607, 610
BccI CCATC 2 cut(s) 178, 491
BciT130I CCWGG 3 cut(s) 181, 346, 410
BciVI GTATCC 1 cut(s) 489
BclI TGATCA 2 cut(s) 397, 445
BcuI ACTAGT 1 cut(s) 419
BfaI CTAG 4 cut(s) 315, 420, 648, 687
BfmI CTRYAG 1 cut(s) 374
BfuI GTATCC 1 cut(s) 489
BisI GCNGC 2 cut(s) 596, 599
BlsI GCNGC 2 cut(s) 597, 600
BmcAI AGTACT 1 cut(s) 424
Bme1390I CCNGG 3 cut(s) 181, 346, 410
Bme18I GGWCC 1 cut(s) 500
BmeRI GACNNNNNGTC 2 cut(s) 661, 810
BmgT120I GGNCC 1 cut(s) 500
BmiI GGNNCC 3 cut(s) 350, 510, 552
BmrFI CCNGG 3 cut(s) 181, 346, 410
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 1 cut(s) 625
BmuI ACTGGG 1 cut(s) 584
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 154
BsaWI WCCGGW 2 cut(s) 307, 559
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 3 cut(s) 430, 590, 623
Bse21I CCTNAGG 1 cut(s) 768
BseBI CCWGG 3 cut(s) 181, 346, 410
BseDI CCNNGG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 3 cut(s) 430, 590, 623
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 2 cut(s) 607, 610
BsgI GTGCAG 2 cut(s) 614, 654
BshFI GGCC 2 cut(s) 408, 719
BshNI GGYRCC 1 cut(s) 348
BshTI ACCGGT 1 cut(s) 307
BsiSI CCGG 2 cut(s) 308, 560
BslFI GGGAC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 28
BsmI GAATGC 3 cut(s) 127, 574, 696
BsnI GGCC 2 cut(s) 408, 719
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 4 cut(s) 397, 445, 508, 550
BspACI CCGC 2 cut(s) 105, 545
BspANI GGCC 2 cut(s) 408, 719
BspCNI CTCAG 1 cut(s) 316
BspHI TCATGA 3 cut(s) 220, 712, 786
BspLI GGNNCC 3 cut(s) 350, 510, 552
BspMAI CTGCAG 1 cut(s) 378
BspPI GGATC 4 cut(s) 503, 516, 545, 558
BspT107I GGYRCC 1 cut(s) 348
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 3 cut(s) 430, 590, 623
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 4 cut(s) 397, 445, 508, 550
BssT1I CCWWGG 1 cut(s) 154
Bst2UI CCWGG 3 cut(s) 181, 346, 410
Bst4CI ACNGT 4 cut(s) 144, 197, 276, 294
Bst6I CTCTTC 1 cut(s) 154
BstDEI CTNAG 2 cut(s) 324, 768
BstKTI GATC 4 cut(s) 400, 448, 511, 553
BstMBI GATC 4 cut(s) 397, 445, 508, 550
BstNI CCWGG 3 cut(s) 181, 346, 410
BstSCI CCNGG 3 cut(s) 179, 344, 408
BstSFI CTRYAG 1 cut(s) 374
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 607, 610
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 2 cut(s) 508, 550
BstXI CCANNNNNNTGG 1 cut(s) 746
BstYI RGATCY 2 cut(s) 508, 550
Bsu36I CCTNAGG 1 cut(s) 768
BsuI GTATCC 1 cut(s) 489
BsuRI GGCC 2 cut(s) 408, 719
BtsIMutI CAGTG 3 cut(s) 272, 278, 597
CciI TCATGA 3 cut(s) 220, 712, 786
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 1 cut(s) 500
Csp6I GTAC 2 cut(s) 423, 626
CspAI ACCGGT 1 cut(s) 307
CviAII CATG 5 cut(s) 221, 449, 713, 787, 815
CviQI GTAC 2 cut(s) 423, 626
DdeI CTNAG 2 cut(s) 324, 768
DpnI GATC 4 cut(s) 399, 447, 510, 552
DpnII GATC 4 cut(s) 397, 445, 508, 550
DrdI GACNNNNNNGTC 1 cut(s) 466
DriI GACNNNNNGTC 2 cut(s) 661, 810
DseDI GACNNNNNNGTC 1 cut(s) 466
Eam1104I CTCTTC 1 cut(s) 154
Eam1105I GACNNNNNGTC 2 cut(s) 661, 810
EarI CTCTTC 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 154
Eco147I AGGCCT 1 cut(s) 719
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 500
Eco57I CTGAAG 2 cut(s) 18, 192
Eco81I CCTNAGG 1 cut(s) 768
EcoRII CCWGG 3 cut(s) 179, 344, 408
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 5 cut(s) 224, 452, 716, 790, 818
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 1 cut(s) 28
FatI CATG 5 cut(s) 220, 448, 712, 786, 814
FbaI TGATCA 2 cut(s) 397, 445
Fnu4HI GCNGC 2 cut(s) 596, 599
Fsp4HI GCNGC 2 cut(s) 596, 599
FspBI CTAG 4 cut(s) 315, 420, 648, 687
GluI GCNGC 2 cut(s) 596, 599
GsuI CTGGAG 1 cut(s) 654
HaeIII GGCC 2 cut(s) 408, 719
HapII CCGG 2 cut(s) 308, 560
Hin1II CATG 5 cut(s) 224, 452, 716, 790, 818
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HindIII AAGCTT 1 cut(s) 799
HinfI GANTC 7 cut(s) 89, 269, 356, 607, 863, 889, 895
HpaII CCGG 2 cut(s) 308, 560
Hpy166II GTNNAC 2 cut(s) 214, 736
Hpy188I TCNGA 7 cut(s) 201, 402, 442, 472, 583, 871, 894
Hpy188III TCNNGA 9 cut(s) 62, 78, 221, 365, 383, 506, 633, 713, 787
Hpy8I GTNNAC 2 cut(s) 214, 736
HpyAV CCTTC 3 cut(s) 684, 784, 866
HpyCH4III ACNGT 4 cut(s) 144, 197, 276, 294
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 4 cut(s) 127, 376, 595, 671
HpyF3I CTNAG 2 cut(s) 324, 768
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 5 cut(s) 224, 452, 716, 790, 818
Ksp22I TGATCA 2 cut(s) 397, 445
Kzo9I GATC 4 cut(s) 397, 445, 508, 550
LmnI GCTCC 1 cut(s) 520
Lsp1109I GCAGC 2 cut(s) 607, 610
LweI GCATC 1 cut(s) 625
MaeI CTAG 4 cut(s) 315, 420, 648, 687
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 6 cut(s) 5, 135, 255, 276, 294, 317
MalI GATC 4 cut(s) 399, 447, 510, 552
MboI GATC 4 cut(s) 397, 445, 508, 550
MboII GAAGA 6 cut(s) 24, 141, 198, 570, 898, 917
MflI RGATCY 2 cut(s) 508, 550
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 6 cut(s) 70, 237, 360, 486, 639, 913
MlyI GAGTC 2 cut(s) 872, 889
MmeI TCCRAC 1 cut(s) 677
MnlI CCTC 7 cut(s) 69, 157, 228, 513, 556, 676, 709
Mph1103I ATGCAT 1 cut(s) 129
MseI TTAA 2 cut(s) 485, 779
MspI CCGG 2 cut(s) 308, 560
MspR9I CCNGG 3 cut(s) 181, 346, 410
Mva1269I GAATGC 3 cut(s) 127, 574, 696
MvaI CCWGG 3 cut(s) 181, 346, 410
NdeII GATC 4 cut(s) 397, 445, 508, 550
NlaIII CATG 5 cut(s) 224, 452, 716, 790, 818
NlaIV GGNNCC 3 cut(s) 350, 510, 552
NmuCI GTSAC 1 cut(s) 276
NsiI ATGCAT 1 cut(s) 129
PagI TCATGA 3 cut(s) 220, 712, 786
PceI AGGCCT 1 cut(s) 719
PctI GAATGC 3 cut(s) 127, 574, 696
PfeI GAWTC 5 cut(s) 89, 269, 356, 607, 889
PinAI ACCGGT 1 cut(s) 307
PkrI GCNGC 2 cut(s) 597, 600
PleI GAGTC 2 cut(s) 871, 889
PpsI GAGTC 2 cut(s) 871, 889
PshBI ATTAAT 1 cut(s) 485
Psp6I CCWGG 3 cut(s) 179, 344, 408
PspGI CCWGG 3 cut(s) 179, 344, 408
PspN4I GGNNCC 3 cut(s) 350, 510, 552
PspPI GGNCC 1 cut(s) 500
PstI CTGCAG 1 cut(s) 378
PsuI RGATCY 2 cut(s) 508, 550
RsaI GTAC 2 cut(s) 424, 627
RsaNI GTAC 2 cut(s) 423, 626
SaqAI TTAA 2 cut(s) 485, 779
SatI GCNGC 2 cut(s) 596, 599
Sau3AI GATC 4 cut(s) 397, 445, 508, 550
Sau96I GGNCC 1 cut(s) 500
ScaI AGTACT 1 cut(s) 424
SchI GAGTC 2 cut(s) 872, 889
ScrFI CCNGG 3 cut(s) 181, 346, 410
SduI GDGCHC 1 cut(s) 353
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 374
SgrAI CRCCGGYG 1 cut(s) 307
SinI GGWCC 1 cut(s) 500
SpeI ACTAGT 1 cut(s) 419
Sse9I AATT 6 cut(s) 70, 237, 360, 486, 639, 913
SseBI AGGCCT 1 cut(s) 719
SsiI CCGC 2 cut(s) 105, 545
SspMI CTAG 4 cut(s) 315, 420, 648, 687
StuI AGGCCT 1 cut(s) 719
StyD4I CCNGG 3 cut(s) 179, 344, 408
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 4 cut(s) 144, 197, 276, 294
TaiI ACGT 1 cut(s) 786
TaqI TCGA 1 cut(s) 527
TasI AATT 6 cut(s) 70, 237, 360, 486, 639, 913
TatI WGTACW 2 cut(s) 422, 625
TfiI GAWTC 5 cut(s) 89, 269, 356, 607, 889
Tru1I TTAA 2 cut(s) 485, 779
Tru9I TTAA 2 cut(s) 485, 779
TscAI CASTG 3 cut(s) 279, 285, 597
TseFI GTSAC 1 cut(s) 276
TseI GCWGC 2 cut(s) 595, 598
Tsp45I GTSAC 1 cut(s) 276
TspDTI ATGAA 3 cut(s) 237, 701, 803
TspRI CASTG 3 cut(s) 279, 285, 597
VpaK11BI GGWCC 1 cut(s) 500
VspI ATTAAT 1 cut(s) 485
XapI RAATTY 2 cut(s) 237, 639
XspI CTAG 4 cut(s) 315, 420, 648, 687
ZrmI AGTACT 1 cut(s) 424
Zsp2I ATGCAT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.