Rh3DG360200

calcium-binding protein CML27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0009728
Physical Location & Seq
Reverse (-)
44307180 .. 44307440
261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG360200.1

Sequence Viewer

Length: 261 bp
ATGGTGGGTGATCCATCTGGAAGTGTACATATACTTATTGCACCAGTCCAAACCCGTCGTGACCGACCACTCGACGAGGTCCACCGCGTCTTCAAGAGCTTCGACTCCAACGGCGACTGCAAGATCTCAACCTCGAAGCTCGGCAACGTCCTCAAGGCCCTTGGCTCTAACATCCCCGAGGACGAGCTCCAACGCATGATGGTCGACCTCGATGGTCCACTGAGATCGTCGTTGACGGTGAACTTCTGGAGGGAGATTTGA

Protein Analysis

86

Amino Acids

9.61

Weight (kDa)

6.05

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 71 2.3e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 204
AccII CGCG 1 cut(s) 87
AciI CCGC 1 cut(s) 85
AclWI GGATC 1 cut(s) 5
AfaI GTAC 1 cut(s) 27
AgsI TTSAA 1 cut(s) 94
AluBI AGCT 3 cut(s) 99, 139, 187
AluI AGCT 3 cut(s) 99, 139, 187
Alw21I GWGCWC 1 cut(s) 189
AlwI GGATC 1 cut(s) 5
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 1 cut(s) 156
AspS9I GGNCC 3 cut(s) 79, 157, 215
AsuHPI GGTGA 2 cut(s) 20, 250
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 2 cut(s) 79, 215
BanII GRGCYC 1 cut(s) 189
BbsI GAAGAC 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 189
BccI CCATC 3 cut(s) 22, 193, 206
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BglII AGATCT 1 cut(s) 123
Bme18I GGWCC 2 cut(s) 79, 215
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 3 cut(s) 79, 157, 215
BpiI GAAGAC 1 cut(s) 82
BpuEI CTTGAG 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 160, 177
Bse1I ACTGG 1 cut(s) 44
BseDI CCNNGG 2 cut(s) 160, 177
BseGI GGATG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 44
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 176
BsnI GGCC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 189
Bsp1407I TGTACA 1 cut(s) 25
Bsp143I GATC 3 cut(s) 10, 123, 224
BspACI CCGC 1 cut(s) 85
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 1 cut(s) 213
BspFNI CGCG 1 cut(s) 87
BspPI GGATC 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 25
BsrI ACTGG 1 cut(s) 44
BssECI CCNNGG 2 cut(s) 160, 177
BssMI GATC 3 cut(s) 10, 123, 224
BssT1I CCWWGG 1 cut(s) 160
Bst4CI ACNGT 1 cut(s) 238
BstAUI TGTACA 1 cut(s) 25
BstDEI CTNAG 1 cut(s) 221
BstF5I GGATG 1 cut(s) 171
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 3 cut(s) 13, 126, 227
BstMBI GATC 3 cut(s) 10, 123, 224
BstUI CGCG 1 cut(s) 87
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 1 cut(s) 158
BtsCI GGATG 1 cut(s) 171
BtsIMutI CAGTG 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 79, 157, 215
CseI GACGC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 26
CviAII CATG 1 cut(s) 196
CviJI RGCY 5 cut(s) 99, 139, 158, 165, 187
CviKI_1 RGCY 5 cut(s) 99, 139, 158, 165, 187
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 1 cut(s) 221
DpnI GATC 3 cut(s) 12, 125, 226
DpnII GATC 3 cut(s) 10, 123, 224
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 79, 215
Eco53kI GAGCTC 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 1 cut(s) 199
FaiI YATR 3 cut(s) 30, 32, 197
FatI CATG 1 cut(s) 195
FblI GTMKAC 1 cut(s) 204
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 1 cut(s) 189
HaeIII GGCC 1 cut(s) 158
HgaI GACGC 1 cut(s) 76
Hin1II CATG 1 cut(s) 199
HincII GTYRAC 2 cut(s) 205, 234
HindII GTYRAC 2 cut(s) 205, 234
HinfI GANTC 1 cut(s) 104
HphI GGTGA 2 cut(s) 20, 250
Hpy166II GTNNAC 6 cut(s) 26, 82, 205, 218, 234, 241
Hpy188III TCNNGA 4 cut(s) 18, 59, 94, 247
Hpy8I GTNNAC 6 cut(s) 26, 82, 205, 218, 234, 241
Hpy99I CGWCG 3 cut(s) 60, 77, 232
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 2 cut(s) 41, 120
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 1 cut(s) 199
Kzo9I GATC 3 cut(s) 10, 123, 224
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 3 cut(s) 3, 57, 232
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 59
MalI GATC 3 cut(s) 12, 125, 226
MboI GATC 3 cut(s) 10, 123, 224
MboII GAAGA 1 cut(s) 82
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 2 cut(s) 132, 214
MnlI CCTC 6 cut(s) 70, 142, 161, 172, 218, 243
MvnI CGCG 1 cut(s) 87
NdeII GATC 3 cut(s) 10, 123, 224
NlaIII CATG 1 cut(s) 199
NmeAIII GCCGAG 1 cut(s) 120
NmuCI GTSAC 1 cut(s) 59
PflFI GACNNNGTC 1 cut(s) 77
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 189
PspPI GGNCC 3 cut(s) 79, 157, 215
PsuI RGATCY 1 cut(s) 123
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
SacI GAGCTC 1 cut(s) 189
SalI GTCGAC 1 cut(s) 203
Sau3AI GATC 3 cut(s) 10, 123, 224
Sau96I GGNCC 3 cut(s) 79, 157, 215
SchI GAGTC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 189
SetI ASST 7 cut(s) 81, 101, 134, 141, 150, 189, 210
SinI GGWCC 2 cut(s) 79, 215
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SsiI CCGC 1 cut(s) 85
SstI GAGCTC 1 cut(s) 189
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 150
TaqI TCGA 5 cut(s) 72, 102, 134, 204, 210
TaqII GACCGA 1 cut(s) 78
TatI WGTACW 1 cut(s) 25
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 59
TspRI CASTG 1 cut(s) 225
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 2 cut(s) 79, 215
XmiI GTMKAC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.