Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0003303

Physical Location & Seq

Genomic Coordinates Forward (+)

46727474 .. 46729333

Gene Structure 1860 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG369200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGAACGCTTTAGTAGCAACAAACAGAAATTTTAAGTTGGCTGCTCGACTGTTGGGATTGGACTCGAAGCTTGAACAGAGTTTACTCATACCATTTAGGGAAATCAAGGTCGAGTGCACTATCCCCAAAGATGATGGCAGTTTGGCTTCATATGTTGGCTTCAGAATTCAACATGACAATGCTAGAGGCCCCATGAAGGGAGGCATCAGATATCACCCAGAGGTTGAGCCAGACGAGGTGAATGCCTTAGCACAGCTGATGACATGGAAGACGGCGGTAGCTAACATCCCATATGGTGGTGCCAAGGGTGGGATAGGATGTGATCCAGGGAAGCTCAGTATTTCAGAGCTTGAGAGACTCACCAGAGTTTTCACCCAAAAGATTCATGATCTTATTGGAATCCACACTGATGTTCCAGCACCAGATATGGGCACTGGTCCACAGACCATGGCTTGGATACTTGATGAGTACTCCAAATTCCATGGCTACTCACCTGCAGTTGTCACTGGGAAACCTACTGATCTTGGTGGATCATTGGGAAGAGATGCAGCAACTGGAAGAGGAGTACTTTTTGCAACAGAGGCTTTACTGAATGAGTATGGGAAGACCATCTCCGGACAACGCTTTGTTATACAGGGATTTGGGAATGTGGGATCCTGGGCTGCCCAACTCATTCATGAGAAAGGTGGGAAAATAGTTGCAGTGAGTGACATCAGTGGAGCTATAAAGAACAGCCAAGGACTTGACATTCCAAGCCTACTCAACCATGTCAAGGAACAAAGAGGTGTCAAGGGCTTCCATGGCGGGGAGTCCATACAAGATTCCCACTCAATTTTAACTGAAGACTGTGACATTCTCATTCCTGCAGCACTTGGAGGTGTCATCAACAGGGAAAATGCAAATGATATAAAAGCCAAATTTATTATCGAAGCAGCTAATCATCCCACTGACCCCGAAGCGGATGAGATCTTGTCAAAGAAAGGTGTTGTTATTCTTCCTGATATATATGCAAACTCTGGAGGAGTCACTGTTAGTTACTTCGAATGGGTGCAGAACATCCAAGGTTTCCTGTGGGATGAAGAAAAAGTGAATAATGAGCTGAGGACTTACATGACTAGAGGCTTCAAAAATGTCAAGGAGATGTGCAAAACCCACAACTGTGACCTCCGGATGGGAGCCTTCACTCTCGGAGTTAATCGCGTTGCACGGGCTACTGTCCTCAGGGGGTGGGAAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004353 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006807 GO:0006950 GO:0006970 GO:0006995 GO:0007154 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009267 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0009991 GO:0010035 GO:0010038 GO:0016491 GO:0016638 GO:0016639 GO:0017076 GO:0019222 GO:0030554 GO:0031667 GO:0031668 GO:0031669 GO:0032553 GO:0032555 GO:0032559 GO:0033554 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0050789 GO:0050896 GO:0050897 GO:0051171 GO:0051716 GO:0055114 GO:0065007 GO:0071496 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00471 ko00910 ko01100 ko01200 ko04217 ko04964 map00220 map00250 map00471 map00910 map01100 map01200 map04217 map04964

Pfam Domains

Protein Families

ELFV_dehydrog ELFV_dehydrog_N

Protein Analysis

412

Amino Acids

44.78

Weight (kDa)

6.28

Isoelectric Point (pI)

27.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ELFV_dehydrog_N	PF02812	33 - 159	4.7e-50	Glu/Leu/Phe/Val dehydrogenase, dimerisation domain
ELFV_dehydrog	PF00208	176 - 409	7.9e-83	Glutamate/Leucine/Phenylalanine/Valine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003303)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03910 AT3G03910 AT5G18170
fragaria_vesca	FvH4_1g28120 FvH4_1g28120 FvH4_1g28120 FvH4_1g28120 FvH4_1g28120 FvH4_1g28120 FvH4_1g28120
malus_domestica	MD12G1003600.v1.1 MD14G1002500.v1.1
prunus_persica	Prupe.7G004100_v2.0.a1 Prupe.7G004100_v2.0.a1 Prupe.7G004100_v2.0.a1 Prupe.7G004100_v2.0.a1 Prupe.7G004100_v2.0.a1
pyrus_communis	pycom12g00300 pycom14g00020
rosa_chinensis	RchiOBHm_Chr3g0497101
rosa_laevigata	RLG00000022481
rosa_multiflora	Rmu_ssc0000003.1_g000006
rosa_roxburghii	Rroxscaffold_6G00388400
rosa_rugosa	Rorug03G0289600 Rorug03G0289700 Rorug03G0289800 Rorug03G0289900 Rorug03G0290000 Rorug03G0290100 Rorug03G0290100 Rorug03G0290200
rosa_samantha	Rh3AG334100 Rh3BG371200 Rh3CG368200 Rh3DG369200
rosa_wichuraiana	Rw3G029340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	502
Acc36I	ACCTGC	1 cut(s)	502
AccB1I	GGYRCC	1 cut(s)	299
AccB7I	CCANNNNNTGG	2 cut(s)	296, 453
AccII	CGCG	1 cut(s)	1200
AccIII	TCCGGA	2 cut(s)	614, 1167
AciI	CCGC	3 cut(s)	275, 804, 959
AclWI	GGATC	4 cut(s)	317, 538, 648, 661
AcsI	RAATTY	4 cut(s)	28, 165, 476, 917
AcuI	CTGAAG	2 cut(s)	145, 861
AfaI	GTAC	2 cut(s)	470, 567
AgsI	TTSAA	3 cut(s)	74, 170, 1126
AjnI	CCWGG	2 cut(s)	325, 656
AleI	CACNNNNGTG	1 cut(s)	1158
AluBI	AGCT	8 cut(s)	70, 256, 281, 334, 349, 722, 935, 1099
AluI	AGCT	8 cut(s)	70, 256, 281, 334, 349, 722, 935, 1099
Alw21I	GWGCWC	1 cut(s)	119
Alw26I	GTCTC	1 cut(s)	349
Alw44I	GTGCAC	1 cut(s)	115
AlwI	GGATC	4 cut(s)	317, 538, 648, 661
AlwNI	CAGNNNCTG	1 cut(s)	554
Aor13HI	TCCGGA	2 cut(s)	614, 1167
AoxI	GGCC	1 cut(s)	187
ApaLI	GTGCAC	1 cut(s)	115
ApeKI	GCWGC	5 cut(s)	41, 548, 662, 866, 932
ApoI	RAATTY	4 cut(s)	28, 165, 476, 917
AspS9I	GGNCC	2 cut(s)	188, 437
AsuHPI	GGTGA	5 cut(s)	206, 250, 352, 364, 483
AsuII	TTCGAA	1 cut(s)	1041
AvaII	GGWCC	1 cut(s)	437
AxyI	CCTNAGG	1 cut(s)	1220
BaeGI	GKGCMC	2 cut(s)	119, 434
BamHI	GGATCC	1 cut(s)	653
BanI	GGYRCC	1 cut(s)	299
BbsI	GAAGAC	3 cut(s)	275, 611, 849
Bbv12I	GWGCWC	1 cut(s)	119
BbvCI	CCTCAGC	1 cut(s)	1100
BbvI	GCAGC	5 cut(s)	28, 560, 649, 878, 944
BccI	CCATC	3 cut(s)	128, 617, 1165
BceAI	ACGGC	1 cut(s)	288
BcgI	CGANNNNNNTGC	2 cut(s)	224, 258
BciT130I	CCWGG	2 cut(s)	327, 658
BciVI	GTATCC	1 cut(s)	450
BcoDI	GTCTC	1 cut(s)	349
BfaI	CTAG	2 cut(s)	183, 1116
BfmI	CTRYAG	2 cut(s)	495, 864
BfuAI	ACCTGC	1 cut(s)	502
BfuI	GTATCC	1 cut(s)	450
BglII	AGATCT	1 cut(s)	966
BisI	GCNGC	5 cut(s)	42, 549, 663, 867, 933
BlsI	GCNGC	5 cut(s)	43, 550, 664, 868, 934
BmcAI	AGTACT	2 cut(s)	470, 567
Bme1390I	CCNGG	2 cut(s)	327, 658
Bme18I	GGWCC	1 cut(s)	437
BmgT120I	GGNCC	2 cut(s)	188, 437
BmiI	GGNNCC	4 cut(s)	190, 301, 655, 1177
BmrFI	CCNGG	2 cut(s)	327, 658
BmrI	ACTGGG	1 cut(s)	516
BmsI	GCATC	2 cut(s)	213, 535
BmuI	ACTGGG	1 cut(s)	516
BpiI	GAAGAC	3 cut(s)	275, 611, 849
BpmI	CTGGAG	1 cut(s)	1038
Bpu10I	CCTNAGC	2 cut(s)	247, 1100
Bpu14I	TTCGAA	1 cut(s)	1041
BpuEI	CTTGAG	1 cut(s)	371
BsaJI	CCNNGG	8 cut(s)	303, 326, 447, 481, 657, 736, 799, 1060
BsaWI	WCCGGW	2 cut(s)	614, 1167
BsaXI	ACNNNNNCTCC	2 cut(s)	1014, 1044
Bse1I	ACTGG	3 cut(s)	439, 511, 559
Bse21I	CCTNAGG	1 cut(s)	1220
BseAI	TCCGGA	2 cut(s)	614, 1167
BseBI	CCWGG	2 cut(s)	327, 658
BseDI	CCNNGG	8 cut(s)	303, 326, 447, 481, 657, 736, 799, 1060
BseGI	GGATG	7 cut(s)	285, 323, 940, 967, 1056, 1081, 1176
BseMII	CTCAG	3 cut(s)	349, 1091, 1234
BseNI	ACTGG	3 cut(s)	439, 511, 559
BseRI	GAGGAG	2 cut(s)	576, 1035
BseSI	GKGCMC	2 cut(s)	119, 434
BseXI	GCAGC	5 cut(s)	28, 560, 649, 878, 944
BsgI	GTGCAG	1 cut(s)	1070
Bsh1236I	CGCG	1 cut(s)	1200
BshFI	GGCC	1 cut(s)	189
BshNI	GGYRCC	1 cut(s)	299
BsiHKAI	GWGCWC	1 cut(s)	119
BsiSI	CCGG	2 cut(s)	615, 1168
BsmAI	GTCTC	1 cut(s)	349
BsmI	GAATGC	1 cut(s)	247
BsnI	GGCC	1 cut(s)	189
Bsp119I	TTCGAA	1 cut(s)	1041
Bsp1286I	GDGCHC	2 cut(s)	119, 434
Bsp13I	TCCGGA	2 cut(s)	614, 1167
Bsp143I	GATC	6 cut(s)	322, 388, 520, 530, 653, 966
Bsp19I	CCATGG	3 cut(s)	447, 481, 799
BspACI	CCGC	3 cut(s)	275, 804, 959
BspANI	GGCC	1 cut(s)	189
BspCNI	CTCAG	3 cut(s)	348, 1092, 1233
BspEI	TCCGGA	2 cut(s)	614, 1167
BspFNI	CGCG	1 cut(s)	1200
BspHI	TCATGA	2 cut(s)	385, 676
BspLI	GGNNCC	4 cut(s)	190, 301, 655, 1177
BspMAI	CTGCAG	2 cut(s)	499, 868
BspMI	ACCTGC	1 cut(s)	502
BspPI	GGATC	4 cut(s)	317, 538, 648, 661
BspT104I	TTCGAA	1 cut(s)	1041
BspT107I	GGYRCC	1 cut(s)	299
BsrI	ACTGG	3 cut(s)	439, 511, 559
BssECI	CCNNGG	8 cut(s)	303, 326, 447, 481, 657, 736, 799, 1060
BssMI	GATC	6 cut(s)	322, 388, 520, 530, 653, 966
BssT1I	CCWWGG	6 cut(s)	303, 447, 481, 736, 799, 1060
Bst2UI	CCWGG	2 cut(s)	327, 658
Bst4CI	ACNGT	5 cut(s)	51, 848, 1030, 1160, 1216
Bst6I	CTCTTC	2 cut(s)	535, 553
BstBI	TTCGAA	1 cut(s)	1041
BstDEI	CTNAG	4 cut(s)	247, 335, 1100, 1220
BstDSI	CCRYGG	3 cut(s)	447, 481, 799
BstF5I	GGATG	7 cut(s)	285, 323, 940, 967, 1056, 1081, 1176
BstFNI	CGCG	1 cut(s)	1200
BstKTI	GATC	6 cut(s)	325, 391, 523, 533, 656, 969
BstMAI	GTCTC	1 cut(s)	349
BstMBI	GATC	6 cut(s)	322, 388, 520, 530, 653, 966
BstMWI	GCNNNNNNNGC	3 cut(s)	14, 581, 801
BstNI	CCWGG	2 cut(s)	327, 658
BstSCI	CCNGG	2 cut(s)	325, 656
BstSFI	CTRYAG	2 cut(s)	495, 864
BstSLI	GKGCMC	2 cut(s)	119, 434
BstUI	CGCG	1 cut(s)	1200
BstV1I	GCAGC	5 cut(s)	28, 560, 649, 878, 944
BstV2I	GAAGAC	3 cut(s)	275, 611, 849
BstX2I	RGATCY	2 cut(s)	653, 966
BstYI	RGATCY	2 cut(s)	653, 966
Bsu36I	CCTNAGG	1 cut(s)	1220
BsuI	GTATCC	1 cut(s)	450
BsuRI	GGCC	1 cut(s)	189
BtgI	CCRYGG	3 cut(s)	447, 481, 799
BtsCI	GGATG	7 cut(s)	285, 323, 940, 967, 1056, 1081, 1176
BtsI	GCAGTG	1 cut(s)	708
BtsIMutI	CAGTG	7 cut(s)	405, 432, 504, 708, 721, 945, 1026
BveI	ACCTGC	1 cut(s)	502
CaiI	CAGNNNCTG	1 cut(s)	554
CciI	TCATGA	2 cut(s)	385, 676
Cfr13I	GGNCC	2 cut(s)	188, 437
Csp6I	GTAC	2 cut(s)	469, 566
CviQI	GTAC	2 cut(s)	469, 566
DdeI	CTNAG	4 cut(s)	247, 335, 1100, 1220
DpnI	GATC	6 cut(s)	324, 390, 522, 532, 655, 968
DpnII	GATC	6 cut(s)	322, 388, 520, 530, 653, 966
Eam1104I	CTCTTC	2 cut(s)	535, 553
EarI	CTCTTC	2 cut(s)	535, 553
Eco130I	CCWWGG	6 cut(s)	303, 447, 481, 736, 799, 1060
Eco32I	GATATC	1 cut(s)	212
Eco47I	GGWCC	1 cut(s)	437
Eco57I	CTGAAG	2 cut(s)	145, 861
Eco81I	CCTNAGG	1 cut(s)	1220
EcoO109I	RGGNCCY	1 cut(s)	188
EcoRI	GAATTC	1 cut(s)	165
EcoRII	CCWGG	2 cut(s)	325, 656
EcoRV	GATATC	1 cut(s)	212
EcoT14I	CCWWGG	6 cut(s)	303, 447, 481, 736, 799, 1060
ErhI	CCWWGG	6 cut(s)	303, 447, 481, 736, 799, 1060
FauI	CCCGC	1 cut(s)	797
FauNDI	CATATG	2 cut(s)	151, 292
Fnu4HI	GCNGC	5 cut(s)	42, 549, 663, 867, 933
FokI	GGATG	7 cut(s)	272, 330, 927, 974, 1043, 1088, 1183
Fsp4HI	GCNGC	5 cut(s)	42, 549, 663, 867, 933
FspBI	CTAG	2 cut(s)	183, 1116
GluI	GCNGC	5 cut(s)	42, 549, 663, 867, 933
GsuI	CTGGAG	1 cut(s)	1038
HaeIII	GGCC	1 cut(s)	189
HapII	CCGG	2 cut(s)	615, 1168
HindIII	AAGCTT	1 cut(s)	68
HinfI	GANTC	7 cut(s)	62, 357, 382, 399, 809, 821, 1023
HpaII	CCGG	2 cut(s)	615, 1168
HphI	GGTGA	5 cut(s)	206, 250, 352, 364, 483
Hpy166II	GTNNAC	3 cut(s)	83, 117, 440
Hpy188I	TCNGA	4 cut(s)	164, 209, 346, 1190
Hpy188III	TCNNGA	6 cut(s)	386, 615, 677, 998, 1017, 1168
Hpy8I	GTNNAC	3 cut(s)	83, 117, 440
HpyAV	CCTTC	2 cut(s)	190, 1189
HpyCH4III	ACNGT	5 cut(s)	51, 848, 1030, 1160, 1216
HpyF10VI	GCNNNNNNNGC	3 cut(s)	14, 581, 801
HpyF3I	CTNAG	4 cut(s)	247, 335, 1100, 1220
Kpn2I	TCCGGA	2 cut(s)	614, 1167
Kzo9I	GATC	6 cut(s)	322, 388, 520, 530, 653, 966
LmnI	GCTCC	2 cut(s)	719, 1175
Lsp1109I	GCAGC	5 cut(s)	28, 560, 649, 878, 944
LweI	GCATC	2 cut(s)	213, 535
MaeI	CTAG	2 cut(s)	183, 1116
MaeIII	GTNAC	6 cut(s)	502, 707, 848, 1024, 1034, 1160
MalI	GATC	6 cut(s)	324, 390, 522, 532, 655, 968
MboI	GATC	6 cut(s)	322, 388, 520, 530, 653, 966
MboII	GAAGA	7 cut(s)	280, 552, 570, 616, 854, 986, 1091
MflI	RGATCY	2 cut(s)	653, 966
MhlI	GDGCHC	2 cut(s)	119, 434
MluCI	AATT	5 cut(s)	28, 165, 476, 831, 917
MlyI	GAGTC	4 cut(s)	56, 351, 818, 1032
MroI	TCCGGA	2 cut(s)	614, 1167
MseI	TTAA	3 cut(s)	33, 836, 1194
MslI	CAYNNNNRTG	3 cut(s)	177, 408, 1158
MspA1I	CMGCKG	1 cut(s)	256
MspI	CCGG	2 cut(s)	615, 1168
MspR9I	CCNGG	2 cut(s)	327, 658
Mva1269I	GAATGC	1 cut(s)	247
MvaI	CCWGG	2 cut(s)	327, 658
MvnI	CGCG	1 cut(s)	1200
MwoI	GCNNNNNNNGC	3 cut(s)	14, 581, 801
NcoI	CCATGG	3 cut(s)	447, 481, 799
NdeI	CATATG	2 cut(s)	151, 292
NdeII	GATC	6 cut(s)	322, 388, 520, 530, 653, 966
NlaIV	GGNNCC	4 cut(s)	190, 301, 655, 1177
NmuCI	GTSAC	5 cut(s)	502, 707, 848, 1024, 1160
NspV	TTCGAA	1 cut(s)	1041
OliI	CACNNNNGTG	1 cut(s)	1158
PagI	TCATGA	2 cut(s)	385, 676
PaqCI	CACCTGC	1 cut(s)	502
PctI	GAATGC	1 cut(s)	247
PfeI	GAWTC	3 cut(s)	382, 399, 821
PflMI	CCANNNNNTGG	2 cut(s)	296, 453
PkrI	GCNGC	5 cut(s)	43, 550, 664, 868, 934
PleI	GAGTC	4 cut(s)	56, 351, 817, 1031
PpsI	GAGTC	4 cut(s)	56, 351, 817, 1031
Psp6I	CCWGG	2 cut(s)	325, 656
PspGI	CCWGG	2 cut(s)	325, 656
PspN4I	GGNNCC	4 cut(s)	190, 301, 655, 1177
PspPI	GGNCC	2 cut(s)	188, 437
PsrI	GAACNNNNNNTAC	2 cut(s)	66, 98
PstI	CTGCAG	2 cut(s)	499, 868
PstNI	CAGNNNCTG	1 cut(s)	554
PsuI	RGATCY	2 cut(s)	653, 966
PvuII	CAGCTG	1 cut(s)	256
RsaI	GTAC	2 cut(s)	470, 567
RsaNI	GTAC	2 cut(s)	469, 566
RseI	CAYNNNNRTG	3 cut(s)	177, 408, 1158
SaqAI	TTAA	3 cut(s)	33, 836, 1194
SatI	GCNGC	5 cut(s)	42, 549, 663, 867, 933
Sau3AI	GATC	6 cut(s)	322, 388, 520, 530, 653, 966
Sau96I	GGNCC	2 cut(s)	188, 437
ScaI	AGTACT	2 cut(s)	470, 567
SchI	GAGTC	4 cut(s)	56, 351, 818, 1032
ScrFI	CCNGG	2 cut(s)	327, 658
SduI	GDGCHC	2 cut(s)	119, 434
SfaNI	GCATC	2 cut(s)	213, 535
SfcI	CTRYAG	2 cut(s)	495, 864
SfuI	TTCGAA	1 cut(s)	1041
SinI	GGWCC	1 cut(s)	437
SmiMI	CAYNNNNRTG	3 cut(s)	177, 408, 1158
SmlI	CTYRAG	1 cut(s)	350
SmoI	CTYRAG	1 cut(s)	350
Sse9I	AATT	5 cut(s)	28, 165, 476, 831, 917
SsiI	CCGC	3 cut(s)	275, 804, 959
SspMI	CTAG	2 cut(s)	183, 1116
StyD4I	CCNGG	2 cut(s)	325, 656
StyI	CCWWGG	6 cut(s)	303, 447, 481, 736, 799, 1060
TaaI	ACNGT	5 cut(s)	51, 848, 1030, 1160, 1216
TaqI	TCGA	5 cut(s)	46, 65, 111, 927, 1041
TasI	AATT	5 cut(s)	28, 165, 476, 831, 917
TatI	WGTACW	2 cut(s)	468, 565
TfiI	GAWTC	3 cut(s)	382, 399, 821
Tru1I	TTAA	3 cut(s)	33, 836, 1194
Tru9I	TTAA	3 cut(s)	33, 836, 1194
TscAI	CASTG	7 cut(s)	412, 439, 511, 708, 721, 952, 1033
TseFI	GTSAC	5 cut(s)	502, 707, 848, 1024, 1160
TseI	GCWGC	5 cut(s)	41, 548, 662, 866, 932
Tsp45I	GTSAC	5 cut(s)	502, 707, 848, 1024, 1160
TspDTI	ATGAA	6 cut(s)	17, 138, 209, 374, 665, 1092
TspRI	CASTG	7 cut(s)	412, 439, 511, 708, 721, 952, 1033
Van91I	CCANNNNNTGG	2 cut(s)	296, 453
VneI	GTGCAC	1 cut(s)	115
VpaK11BI	GGWCC	1 cut(s)	437
XapI	RAATTY	4 cut(s)	28, 165, 476, 917
XspI	CTAG	2 cut(s)	183, 1116
ZrmI	AGTACT	2 cut(s)	470, 567

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.