Rh4DG195600

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011625
Physical Location & Seq
Reverse (-)
38182182 .. 38187212
5031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG195600.1

Sequence Viewer

Length: 450 bp
ATGCTCTTATTCACCCTCCAATGTTATTACCGTGTGCGACAAATGCTGCAGAAGAGGATGATGGTACACAAAAAAAGGGAACACAAAGGAAATAATGATGAAATCCAAAACACTAGATCTTTGAAAAGCGTAAGGCTGATCATGACACCACTGATAGGCATCATGTTGAGTAACTGCGATGTATCTGTCCGCATGTCTTGCTTGAACACATGGTGTTGTCTATGTAATAGACTTGATACCTCTCTGAATGATTCTTCAATGATTAACCGGGTAGTAGACCCTATGTTTGAAGCAGCTTTTCAACTTGATCCTAATGGTAAGAGCTTCTGGTCTACGGATCTGTGTGTCAATTTGCTTGATAATTTTATATCTGCAAAATGTAAACCTCTAAACTATGACTATCTTGTGGATTTTCTTATTGTATGTAGTAGATTAGCACTATTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

149

Amino Acids

17.31

Weight (kDa)

8.77

Isoelectric Point (pI)

27.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 276, 332
AciI CCGC 1 cut(s) 190
AclWI GGATC 2 cut(s) 302, 345
AdeI CACNNNGTG 1 cut(s) 213
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 5 cut(s) 124, 205, 258, 290, 302
AluBI AGCT 2 cut(s) 296, 324
AluI AGCT 2 cut(s) 296, 324
AlwI GGATC 2 cut(s) 302, 345
ApeKI GCWGC 2 cut(s) 46, 293
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 4
BbvI GCAGC 2 cut(s) 33, 305
BccI CCATC 1 cut(s) 55
BclI TGATCA 1 cut(s) 138
BcnI CCSGG 1 cut(s) 269
BfaI CTAG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 47
BglII AGATCT 1 cut(s) 116
BisI GCNGC 2 cut(s) 47, 294
BlsI GCNGC 2 cut(s) 48, 295
Bme1390I CCNGG 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 1 cut(s) 168
BpuMI CCSGG 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 158
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse8I GATNNNNATC 1 cut(s) 158
BseGI GGATG 1 cut(s) 63
BseJI GATNNNNATC 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 155
BseXI GCAGC 2 cut(s) 33, 305
BsiSI CCGG 1 cut(s) 268
BslI CCNNNNNNNGG 1 cut(s) 155
Bsp143I GATC 4 cut(s) 116, 138, 307, 337
BspACI CCGC 1 cut(s) 190
BspHI TCATGA 1 cut(s) 141
BspMAI CTGCAG 1 cut(s) 51
BspPI GGATC 2 cut(s) 302, 345
BssMI GATC 4 cut(s) 116, 138, 307, 337
Bst4CI ACNGT 1 cut(s) 32
Bst6I CTCTTC 1 cut(s) 47
BstAPI GCANNNNNTGC 1 cut(s) 198
BstF5I GGATG 1 cut(s) 63
BstKTI GATC 4 cut(s) 119, 141, 310, 340
BstMBI GATC 4 cut(s) 116, 138, 307, 337
BstMWI GCNNNNNNNGC 2 cut(s) 43, 198
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 33, 305
BstX2I RGATCY 2 cut(s) 116, 337
BstYI RGATCY 2 cut(s) 116, 337
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 1 cut(s) 63
BtsIMutI CAGTG 1 cut(s) 149
CciI TCATGA 1 cut(s) 141
Csp6I GTAC 1 cut(s) 65
CviAII CATG 4 cut(s) 142, 163, 193, 210
CviJI RGCY 3 cut(s) 136, 296, 324
CviKI_1 RGCY 3 cut(s) 136, 296, 324
CviQI GTAC 1 cut(s) 65
DpnI GATC 4 cut(s) 118, 140, 309, 339
DpnII GATC 4 cut(s) 116, 138, 307, 337
DraIII CACNNNGTG 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
FaeI CATG 4 cut(s) 145, 166, 196, 213
FaiI YATR 9 cut(s) 143, 164, 194, 211, 223, 284, 368, 396, 424
FatI CATG 4 cut(s) 141, 162, 192, 209
FbaI TGATCA 1 cut(s) 138
FblI GTMKAC 2 cut(s) 276, 332
Fnu4HI GCNGC 2 cut(s) 47, 294
FokI GGATG 1 cut(s) 70
Fsp4HI GCNGC 2 cut(s) 47, 294
FspBI CTAG 1 cut(s) 114
GluI GCNGC 2 cut(s) 47, 294
HapII CCGG 1 cut(s) 268
Hin1II CATG 4 cut(s) 145, 166, 196, 213
HinfI GANTC 1 cut(s) 251
HpaII CCGG 1 cut(s) 268
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 4 cut(s) 67, 277, 333, 383
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 1 cut(s) 142
Hpy8I GTNNAC 4 cut(s) 67, 277, 333, 383
HpyCH4III ACNGT 1 cut(s) 32
HpyCH4V TGCA 2 cut(s) 49, 374
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 198
Hsp92II CATG 4 cut(s) 145, 166, 196, 213
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 4 cut(s) 116, 138, 307, 337
LpnPI CCDG 2 cut(s) 281, 313
Lsp1109I GCAGC 2 cut(s) 33, 305
LweI GCATC 1 cut(s) 168
MaeI CTAG 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 170
MalI GATC 4 cut(s) 118, 140, 309, 339
MboI GATC 4 cut(s) 116, 138, 307, 337
MboII GAAGA 2 cut(s) 64, 246
MflI RGATCY 2 cut(s) 116, 337
MluCI AATT 2 cut(s) 349, 361
MnlI CCTC 4 cut(s) 26, 48, 250, 396
MseI TTAA 1 cut(s) 264
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 43, 198
NciI CCSGG 1 cut(s) 269
NdeII GATC 4 cut(s) 116, 138, 307, 337
NlaIII CATG 4 cut(s) 145, 166, 196, 213
NspI RCATGY 1 cut(s) 196
PagI TCATGA 1 cut(s) 141
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 48, 295
PstI CTGCAG 1 cut(s) 51
PsuI RGATCY 2 cut(s) 116, 337
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 2 cut(s) 47, 294
Sau3AI GATC 4 cut(s) 116, 138, 307, 337
ScrFI CCNGG 1 cut(s) 269
SetI ASST 4 cut(s) 242, 298, 326, 388
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 47
Sse9I AATT 2 cut(s) 349, 361
SsiI CCGC 1 cut(s) 190
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 32
TasI AATT 2 cut(s) 349, 361
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 1 cut(s) 156
TseI GCWGC 2 cut(s) 46, 293
TspDTI ATGAA 1 cut(s) 114
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 1 cut(s) 156
XceI RCATGY 1 cut(s) 196
XmiI GTMKAC 2 cut(s) 276, 332
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.