Rh5AG366100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011581
Physical Location & Seq
Forward (+)
61800850 .. 61801458
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG366100.1

Sequence Viewer

Length: 411 bp
ATGGATCGGTTTGTGGCGGTGTTAGCACCAGCTATTTCCTTCTCCGTTGGGTCTGCAATTAGGCTGAACCTGTGGTGGTGCTCCGATGTCAGGATGAATTGGGTGTATTCCAATTTTCCGATGGTAAACAGTACTGTGAAGTCTCTGGGTCACTTAACTAGTCGACAGGTGGCTGGTTATGTTTATGCTGCTGTCCTTTCCTCCCTTGGAGGGTTTGCTACTTGGATGGATAATGATGGGTTGTTCCCACTTATGGAGGGTCCCAAAAAAAAAACTCTTCACCATGCTTCTTGCAAGCGAAGAGAGGTCTTGGCTCAGTCCCAGCAGGAATTTCTCGATGGGTCGCCCAATCCTGTGGTTATGGAGAAAATGGTATATGCTGCTTTTGATTTCTTGCTCTATCTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.23

Weight (kDa)

8.62

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 163
AciI CCGC 1 cut(s) 17
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 329
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 3 cut(s) 90, 210, 253
AhlI ACTAGT 1 cut(s) 158
AluBI AGCT 1 cut(s) 32
AluI AGCT 1 cut(s) 32
Alw21I GWGCWC 1 cut(s) 83
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 188, 380
ApoI RAATTY 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 272
AvaII GGWCC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 175, 367
BccI CCATC 4 cut(s) 115, 220, 230, 332
BcoDI GTCTC 1 cut(s) 147
BcuI ACTAGT 1 cut(s) 158
BfaI CTAG 1 cut(s) 159
BisI GCNGC 2 cut(s) 189, 381
BlsI GCNGC 2 cut(s) 190, 382
BmcAI AGTACT 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 2 cut(s) 261, 262
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 210, 253
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 2 cut(s) 99, 231
BseLI CCNNNNNNNGG 3 cut(s) 90, 210, 253
BseMII CTCAG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 175, 367
BseYI CCCAGC 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 83
BslFI GGGAC 2 cut(s) 246, 304
BslI CCNNNNNNNGG 3 cut(s) 90, 210, 253
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 2 cut(s) 246, 304
Bsp1286I GDGCHC 1 cut(s) 83
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 1 cut(s) 17
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 261, 262
BspPI GGATC 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 1 cut(s) 4
BssT1I CCWWGG 1 cut(s) 205
Bst4CI ACNGT 2 cut(s) 131, 136
Bst6I CTCTTC 2 cut(s) 282, 295
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 99, 231
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstV1I GCAGC 2 cut(s) 175, 367
BstXI CCANNNNNNTGG 1 cut(s) 355
BtsCI GGATG 2 cut(s) 99, 231
Cac8I GCNNGC 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 132
CviAII CATG 1 cut(s) 284
CviJI RGCY 4 cut(s) 32, 64, 173, 314
CviKI_1 RGCY 4 cut(s) 32, 64, 173, 314
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 1 cut(s) 315
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Eam1104I CTCTTC 2 cut(s) 282, 295
EarI CTCTTC 2 cut(s) 282, 295
Eco130I CCWWGG 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 1 cut(s) 287
FaiI YATR 7 cut(s) 180, 186, 254, 285, 362, 376, 378
FaqI GGGAC 2 cut(s) 246, 304
FatI CATG 1 cut(s) 283
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 2 cut(s) 189, 381
FokI GGATG 2 cut(s) 106, 238
Fsp4HI GCNGC 2 cut(s) 189, 381
FspBI CTAG 1 cut(s) 159
GluI GCNGC 2 cut(s) 189, 381
GsaI CCCAGC 1 cut(s) 325
Hin1II CATG 1 cut(s) 287
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 2 cut(s) 127, 164
Hpy188I TCNGA 2 cut(s) 85, 120
Hpy188III TCNNGA 2 cut(s) 91, 335
Hpy8I GTNNAC 2 cut(s) 127, 164
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 2 cut(s) 131, 136
HpyCH4V TGCA 2 cut(s) 56, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 1 cut(s) 315
Hsp92II CATG 1 cut(s) 287
KflI GGGWCCC 1 cut(s) 260
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 9 cut(s) 42, 76, 83, 131, 152, 159, 311, 335, 366
Lsp1109I GCAGC 2 cut(s) 175, 367
MaeI CTAG 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 149
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 2 cut(s) 269, 312
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 4 cut(s) 57, 97, 112, 329
MnlI CCTC 4 cut(s) 203, 211, 250, 298
MseI TTAA 2 cut(s) 155, 409
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 2 cut(s) 261, 262
NmuCI GTSAC 1 cut(s) 149
PkrI GCNGC 2 cut(s) 190, 382
PpuMI RGGWCCY 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 261, 262
PspPI GGNCC 1 cut(s) 260
PspPPI RGGWCCY 1 cut(s) 260
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SalI GTCGAC 1 cut(s) 162
SaqAI TTAA 2 cut(s) 155, 409
SatI GCNGC 2 cut(s) 189, 381
Sau3AI GATC 1 cut(s) 4
Sau96I GGNCC 1 cut(s) 260
ScaI AGTACT 1 cut(s) 133
SduI GDGCHC 1 cut(s) 83
SetI ASST 4 cut(s) 34, 72, 171, 309
SinI GGWCC 1 cut(s) 260
SpeI ACTAGT 1 cut(s) 158
Sse9I AATT 4 cut(s) 57, 97, 112, 329
SsiI CCGC 1 cut(s) 17
SspMI CTAG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 131, 136
TaqI TCGA 2 cut(s) 163, 336
TasI AATT 4 cut(s) 57, 97, 112, 329
TatI WGTACW 1 cut(s) 131
Tru1I TTAA 2 cut(s) 155, 409
Tru9I TTAA 2 cut(s) 155, 409
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 2 cut(s) 188, 380
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 1 cut(s) 110
TspGWI ACGGA 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 1 cut(s) 159
ZrmI AGTACT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.