Rh5BG165700

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0014866
Physical Location & Seq
Forward (+)
17403562 .. 17415004
11443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG165700.1

Sequence Viewer

Length: 546 bp
ATGAAGTCAAGACATTTCCAGAAGGCAGATTACGCAGATAGCAGCAACTTAGATCTTGATCTCAGGTGTCATTTGGCAGAAGTTGAAGAAAACAGAAAGAGGTTCTGGTCTCCTAGAGATGCAGCAGAAGAAGGAGAAGATGAAGAGAAGGGTGGAGAACTGAAGAATAAATCTGGAGATATATATTTTTGTTCAATCATCTGCAATTCCTCTGAGAAGAAAAAGAAGGGGAAAGGAGGCTCTTATAGGAAAGCTAATCCCATTCCTGACTATGTCCATGTACACGAATTTGTGTTTGCCCTCTCGGAGAAACCTGTGTGCTCTATCGAAGGTCATTTGGATGCTGTACTGGACCTATCATGGTCCAGAGATCAGCTATTGCTTTCATCTTCAATGGACAAAACTGTTAGATTATGGGATATGGTGACCAAGAGTTGTTTAAAAATGTTTGCCCACAATGATTATGGTAAGAAGCATCTATACATACATAGTGCAGAAGACCATAAAACAGTTAAACTTGCGGTGGGATGGAGCTGGAGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

181

Amino Acids

20.81

Weight (kDa)

7.11

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 102 - 153 7.8e-09 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 103 - 152 2.4e-06 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 104 - 140 2.1e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 105 - 152 4.9e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 108 - 153 5.8e-08 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 109 - 151 2.6e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 111 - 151 3.3e-06 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 521
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 182
AfaI GTAC 2 cut(s) 282, 348
AgsI TTSAA 3 cut(s) 86, 195, 393
AluBI AGCT 3 cut(s) 254, 376, 534
AluI AGCT 3 cut(s) 254, 376, 534
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 1 cut(s) 114
ApeKI GCWGC 2 cut(s) 42, 122
ApoI RAATTY 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 352, 363
AsuHPI GGTGA 1 cut(s) 436
AvaII GGWCC 2 cut(s) 352, 363
BarI GAAGNNNNNNTAC 4 cut(s) 14, 46, 464, 496
BbsI GAAGAC 1 cut(s) 504
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 2 cut(s) 54, 134
BccI CCATC 1 cut(s) 522
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 114
BglII AGATCT 1 cut(s) 52
BisI GCNGC 2 cut(s) 43, 123
BlsI GCNGC 2 cut(s) 44, 124
Bme18I GGWCC 2 cut(s) 352, 363
BmgT120I GGNCC 2 cut(s) 352, 363
BmsI GCATC 3 cut(s) 109, 331, 484
BpiI GAAGAC 1 cut(s) 504
BpmI CTGGAG 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 354
Bse8I GATNNNNATC 1 cut(s) 57
BseGI GGATG 2 cut(s) 346, 533
BseJI GATNNNNATC 1 cut(s) 57
BseMII CTCAG 2 cut(s) 76, 204
BseNI ACTGG 1 cut(s) 354
BseXI GCAGC 2 cut(s) 54, 134
BsgI GTGCAG 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 114
Bso31I GGTCTC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 323
Bsp1407I TGTACA 1 cut(s) 280
Bsp143I GATC 3 cut(s) 52, 58, 370
BspACI CCGC 1 cut(s) 521
BspCNI CTCAG 2 cut(s) 75, 205
BspTNI GGTCTC 1 cut(s) 114
BsrGI TGTACA 1 cut(s) 280
BsrI ACTGG 1 cut(s) 354
BssMI GATC 3 cut(s) 52, 58, 370
Bst4CI ACNGT 2 cut(s) 406, 511
Bst6I CTCTTC 1 cut(s) 138
BstAUI TGTACA 1 cut(s) 280
BstDEI CTNAG 3 cut(s) 49, 62, 213
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 2 cut(s) 346, 533
BstKTI GATC 3 cut(s) 55, 61, 373
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 3 cut(s) 52, 58, 370
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstPI GGTNACC 1 cut(s) 424
BstV1I GCAGC 2 cut(s) 54, 134
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BtsCI GGATG 2 cut(s) 346, 533
Cfr13I GGNCC 2 cut(s) 352, 363
Csp6I GTAC 2 cut(s) 281, 347
CviAII CATG 2 cut(s) 278, 360
CviJI RGCY 4 cut(s) 240, 254, 376, 534
CviKI_1 RGCY 4 cut(s) 240, 254, 376, 534
CviQI GTAC 2 cut(s) 281, 347
DdeI CTNAG 3 cut(s) 49, 62, 213
DpnI GATC 3 cut(s) 54, 60, 372
DpnII GATC 3 cut(s) 52, 58, 370
DraI TTTAAA 1 cut(s) 441
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 352, 363
Eco57I CTGAAG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
FaeI CATG 2 cut(s) 281, 363
FatI CATG 2 cut(s) 277, 359
Fnu4HI GCNGC 2 cut(s) 43, 123
FokI GGATG 2 cut(s) 353, 540
Fsp4HI GCNGC 2 cut(s) 43, 123
FspBI CTAG 1 cut(s) 114
GluI GCNGC 2 cut(s) 43, 123
GsuI CTGGAG 1 cut(s) 195
Hin1II CATG 2 cut(s) 281, 363
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 2 cut(s) 214, 307
Hpy188III TCNNGA 6 cut(s) 9, 19, 56, 174, 266, 366
Hpy8I GTNNAC 1 cut(s) 283
HpyAV CCTTC 5 cut(s) 16, 125, 142, 220, 323
HpyCH4III ACNGT 2 cut(s) 406, 511
HpyCH4V TGCA 3 cut(s) 122, 204, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 3 cut(s) 49, 62, 213
Hsp92II CATG 2 cut(s) 281, 363
Kzo9I GATC 3 cut(s) 52, 58, 370
LmnI GCTCC 1 cut(s) 531
LpnPI CCDG 9 cut(s) 32, 49, 91, 159, 279, 327, 335, 379, 520
Lsp1109I GCAGC 2 cut(s) 54, 134
LweI GCATC 3 cut(s) 109, 331, 484
MaeI CTAG 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 424
MalI GATC 3 cut(s) 54, 60, 372
MboI GATC 3 cut(s) 52, 58, 370
MboII GAAGA 8 cut(s) 98, 140, 149, 155, 175, 229, 381, 509
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 2 cut(s) 205, 287
MnlI CCTC 4 cut(s) 93, 220, 230, 311
MseI TTAA 2 cut(s) 440, 513
MslI CAYNNNNRTG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 32
NdeII GATC 3 cut(s) 52, 58, 370
NlaIII CATG 2 cut(s) 281, 363
NmuCI GTSAC 1 cut(s) 424
PflFI GACNNNGTC 1 cut(s) 272
PkrI GCNGC 2 cut(s) 44, 124
PspEI GGTNACC 1 cut(s) 424
PspPI GGNCC 2 cut(s) 352, 363
PsuI RGATCY 1 cut(s) 52
PsyI GACNNNGTC 1 cut(s) 272
RsaI GTAC 2 cut(s) 282, 348
RsaNI GTAC 2 cut(s) 281, 347
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 2 cut(s) 440, 513
SatI GCNGC 2 cut(s) 43, 123
Sau3AI GATC 3 cut(s) 52, 58, 370
Sau96I GGNCC 2 cut(s) 352, 363
SduI GDGCHC 1 cut(s) 323
SetI ASST 8 cut(s) 68, 104, 256, 316, 334, 357, 378, 536
SfaNI GCATC 3 cut(s) 109, 331, 484
SinI GGWCC 2 cut(s) 352, 363
SmiMI CAYNNNNRTG 1 cut(s) 339
Sse9I AATT 2 cut(s) 205, 287
SsiI CCGC 1 cut(s) 521
SspMI CTAG 1 cut(s) 114
TaaI ACNGT 2 cut(s) 406, 511
TaqI TCGA 1 cut(s) 327
TasI AATT 2 cut(s) 205, 287
TatI WGTACW 2 cut(s) 280, 346
Tru1I TTAA 2 cut(s) 440, 513
Tru9I TTAA 2 cut(s) 440, 513
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 2 cut(s) 42, 122
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 3 cut(s) 17, 156, 375
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 352, 363
XapI RAATTY 1 cut(s) 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 461
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.