Rh5BG227200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010413
Physical Location & Seq
Forward (+)
26687020 .. 26689313
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG227200.1

Sequence Viewer

Length: 1719 bp
ATGGCCAAGTCCTCTTCTTCCCATCGTAAGAAACGCGACAAGATTTCCTCTAAGGCTCGAACGAAGAAGAGGAGTAAGAGTAAGAGCAAGAGCAAGAGCAAGAGTAAGAGTGAGAAATATAGATCCAAGAAGGTTCGTCGTCGCGATGATTCTCTTTCTTTTTCGGATGAGGATGATTCCAGAAGCTTGAGTTCAGATTCGTTGTTTAGCTCTGGGGATGACCGTAGAAGTAGAAGGGCTCGTTCACGCAGTCGGAGGGATGTGAAGGGTAGCAAGAAGAGGGCTCGGAAGCGATCTTATAGCAGCGATAGTAGTGAGGAGTCTCCCCATCCGAAGAAGAGGAAAGCAACAAAGAAGGTTAAGGAGTATGATGCGAAGAAGAGGAGCCGTTCGAGGGAGAAGCCTAAGAGAAATGCTAGGACTAGTTCTGTGAGTAGTGAGTCTTGGAGCTGCTCGACTTGTCAGGGTGGGGGTGTTAGTGGTGACGAGATTGAATCTAAAAGACATAGGGGAAGGTCTGAAAAACGAAAGAGAGAGGAAACCGATAGAAACAAGGTTGAGAGTGGGACTGAGAGGAGTAGATATAGATCTAGGAGTCGTTCTTTGCACAGTCATTATAGCGAGAGTGATTGCCAGAATCAGGAAAGAGTGACCGGAGAGAGCAATGGGAGGAGATTGAGATCAGTTATTGCGGTGGCCGAAGAAAACAATGAAGGCAGATGGTTAGATGAGGATGGGCACAAAGAAGAGATTACATATGATCGTGATGATTACCCTTCTTGTAGAAGTAATGACAGTAATGACGGAGGGAGCAAGAAGGAGTTGGATAATCATCTACATGTTTCATCCGAGGTGAGAATGAGGGTAGAGAGTGTGAAAGATGAAGAAGCTCTTGTTTCTGATGCAGAAGGAATTAATTCTAGCCGTGATGGAACCATGATGACCGATCATGTTAATGAAAATAAGATCTCTGGGGAAAGTAGTAGTTTGAAAGACGATGATCTGGAGACGATCTTAAGACTGAAGGCTTTAGAAAATCTAAAAAGGTTCAGAGGAAAGCCTCAAAGGATTGCAGTAACTGGTAATCAGGAAGATAAGAAGCAACCATCCGGTGCAAGGGCTGAATTGGTCCAAATAGACTCCCCCAAGGAGGATATTTCTGAAATAAATGGTCAAACCCGATTAGCAGAAGAGACCAATGAGCCTCCAGTGAAAGATTCCATTTGTGGAAATGAACCTGTCTCTGCCAAGCAAGATGTTGCTTGTCCAACACATCAAGAGCGTGTTGCTGGTATTTCCAAAATGGTTAGCACTACTGCTGTCGTAGACAAACCAAACTTGGCTGCACCAAAATCAATGTCTGGTTCACTAAAAACTCACTCAACTTTGAAGCCAGCACTTGCTTCTCTGGAACCCCCTCAGGAAAGATTGTTGGTAACTGAGAGTAGTGTAGATAACACAGCTTCTGCTACTGCTCAAACTGTGACCCAAAGTAGCAAAAATGATGCTCTAGACATCAGCAACGGTTCTGGTTCTACTGCTCCTGAACCTTCTGGAGAGAATAGGTCTGGTTTACAGCAAGATGAGGCTAAGGATGGTTCACAGTTTGAGCAGAAGACCATGTCTGTGATGCGAGGTAGTGAAATGGTACAGGTAAGCTACAAGGTTTACATCCCGAAGAAAGCCCCTGCTTTGGCGAGGAGGCACCTCAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

572

Amino Acids

63.87

Weight (kDa)

9.69

Isoelectric Point (pI)

75.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1704
AccI GTMKAC 1 cut(s) 1326
AccII CGCG 2 cut(s) 36, 144
AciI CCGC 1 cut(s) 692
AclWI GGATC 1 cut(s) 117
AcoI YGGCCR 2 cut(s) 3, 696
AcuI CTGAAG 1 cut(s) 1043
AfaI GTAC 1 cut(s) 1650
AfiI CCNNNNNNNGG 5 cut(s) 394, 640, 1116, 1150, 1693
AflII CTTAAG 1 cut(s) 1015
AflIII ACRYGT 1 cut(s) 838
AgsI TTSAA 3 cut(s) 494, 991, 1390
AhlI ACTAGT 1 cut(s) 422
AluBI AGCT 6 cut(s) 186, 210, 450, 890, 1463, 1659
AluI AGCT 6 cut(s) 186, 210, 450, 890, 1463, 1659
Alw26I GTCTC 4 cut(s) 327, 1001, 1187, 1246
AlwI GGATC 1 cut(s) 117
AlwNI CAGNNNCTG 2 cut(s) 1079, 1466
AoxI GGCC 2 cut(s) 3, 696
ApeKI GCWGC 3 cut(s) 303, 450, 1343
ArsI GACNNNNNNTTYG 2 cut(s) 1343, 1375
AseI ATTAAT 1 cut(s) 915
Asp700I GAANNNNTTC 1 cut(s) 916
AspS9I GGNCC 1 cut(s) 1129
AsuHPI GGTGA 2 cut(s) 494, 865
AvaII GGWCC 1 cut(s) 1129
AxyI CCTNAGG 1 cut(s) 1419
BaeGI GKGCMC 1 cut(s) 741
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 1704
BanII GRGCYC 2 cut(s) 241, 286
BbsI GAAGAC 1 cut(s) 1622
BbvI GCAGC 3 cut(s) 315, 437, 1330
BccI CCATC 7 cut(s) 30, 336, 714, 728, 923, 1114, 1589
BceAI ACGGC 2 cut(s) 372, 909
BcoDI GTCTC 4 cut(s) 327, 1001, 1187, 1246
BcuI ACTAGT 1 cut(s) 422
BfaI CTAG 5 cut(s) 417, 423, 591, 921, 1511
BfrI CTTAAG 1 cut(s) 1015
BglII AGATCT 2 cut(s) 587, 966
BisI GCNGC 3 cut(s) 304, 451, 1344
BlsI GCNGC 3 cut(s) 305, 452, 1345
Bme18I GGWCC 1 cut(s) 1129
BmgT120I GGNCC 1 cut(s) 1129
BmiI GGNNCC 4 cut(s) 386, 934, 1413, 1706
BmsI GCATC 4 cut(s) 361, 892, 1495, 1620
BpiI GAAGAC 1 cut(s) 1622
BpmI CTGGAG 3 cut(s) 1025, 1191, 1575
Bpu10I CCTNAGC 1 cut(s) 1590
BpuEI CTTGAG 2 cut(s) 208, 1694
BsaBI GATNNNNATC 3 cut(s) 586, 679, 831
BsaI GGTCTC 1 cut(s) 1187
BsaJI CCNNGG 2 cut(s) 849, 1146
BsaWI WCCGGW 2 cut(s) 653, 1109
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 5 cut(s) 394, 640, 1116, 1150, 1693
Bse1I ACTGG 2 cut(s) 1084, 1208
Bse21I CCTNAGG 1 cut(s) 1419
Bse3DI GCAATG 1 cut(s) 670
Bse8I GATNNNNATC 3 cut(s) 586, 679, 831
BseDI CCNNGG 2 cut(s) 849, 1146
BseJI GATNNNNATC 3 cut(s) 586, 679, 831
BseLI CCNNNNNNNGG 5 cut(s) 394, 640, 1116, 1150, 1693
BseMI GCAATG 1 cut(s) 670
BseMII CTCAG 3 cut(s) 561, 1431, 1433
BseNI ACTGG 2 cut(s) 1084, 1208
BseRI GAGGAG 6 cut(s) 85, 332, 397, 589, 685, 1714
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 3 cut(s) 315, 437, 1330
BsgI GTGCAG 1 cut(s) 1329
Bsh1236I CGCG 2 cut(s) 36, 144
BshFI GGCC 2 cut(s) 5, 698
BshNI GGYRCC 1 cut(s) 1704
BsiSI CCGG 2 cut(s) 654, 1110
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 5 cut(s) 394, 640, 1116, 1150, 1693
BsmAI GTCTC 4 cut(s) 327, 1001, 1187, 1246
BsmBI CGTCTC 1 cut(s) 1001
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 2 cut(s) 5, 698
Bso31I GGTCTC 1 cut(s) 1187
Bsp1286I GDGCHC 3 cut(s) 241, 286, 741
Bsp143I GATC 9 cut(s) 122, 293, 587, 680, 760, 946, 966, 1000, 1011
Bsp68I TCGCGA 1 cut(s) 144
BspACI CCGC 1 cut(s) 692
BspANI GGCC 2 cut(s) 5, 698
BspCNI CTCAG 3 cut(s) 562, 1432, 1432
BspFNI CGCG 2 cut(s) 36, 144
BspLI GGNNCC 4 cut(s) 386, 934, 1413, 1706
BspPI GGATC 1 cut(s) 117
BspT107I GGYRCC 1 cut(s) 1704
BspTI CTTAAG 1 cut(s) 1015
BspTNI GGTCTC 1 cut(s) 1187
BsrDI GCAATG 1 cut(s) 670
BsrI ACTGG 2 cut(s) 1084, 1208
BssECI CCNNGG 2 cut(s) 849, 1146
BssMI GATC 9 cut(s) 122, 293, 587, 680, 760, 946, 966, 1000, 1011
BssT1I CCWWGG 1 cut(s) 1146
Bst4CI ACNGT 6 cut(s) 224, 611, 797, 1483, 1526, 1605
Bst6I CTCTTC 7 cut(s) 19, 62, 272, 332, 374, 741, 1185
BstAFI CTTAAG 1 cut(s) 1015
BstC8I GCNNGC 1 cut(s) 1395
BstDEI CTNAG 6 cut(s) 51, 405, 570, 1419, 1440, 1590
BstFNI CGCG 2 cut(s) 36, 144
BstKTI GATC 9 cut(s) 125, 296, 590, 683, 763, 949, 969, 1003, 1014
BstMAI GTCTC 4 cut(s) 327, 1001, 1187, 1246
BstMBI GATC 9 cut(s) 122, 293, 587, 680, 760, 946, 966, 1000, 1011
BstMWI GCNNNNNNNGC 1 cut(s) 1711
BstNSI RCATGY 1 cut(s) 842
BstSLI GKGCMC 1 cut(s) 741
BstUI CGCG 2 cut(s) 36, 144
BstV1I GCAGC 3 cut(s) 315, 437, 1330
BstV2I GAAGAC 1 cut(s) 1622
BstX2I RGATCY 3 cut(s) 122, 587, 966
BstYI RGATCY 3 cut(s) 122, 587, 966
Bsu36I CCTNAGG 1 cut(s) 1419
BsuRI GGCC 2 cut(s) 5, 698
BtgZI GCGATG 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 1215
BtuMI TCGCGA 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 1395
CaiI CAGNNNCTG 2 cut(s) 1079, 1466
Cfr13I GGNCC 1 cut(s) 1129
Csp6I GTAC 1 cut(s) 1649
CviAII CATG 4 cut(s) 839, 937, 950, 1621
CviQI GTAC 1 cut(s) 1649
DdeI CTNAG 6 cut(s) 51, 405, 570, 1419, 1440, 1590
DpnI GATC 9 cut(s) 124, 295, 589, 682, 762, 948, 968, 1002, 1013
DpnII GATC 9 cut(s) 122, 293, 587, 680, 760, 946, 966, 1000, 1011
EaeI YGGCCR 2 cut(s) 3, 696
Eam1104I CTCTTC 7 cut(s) 19, 62, 272, 332, 374, 741, 1185
EarI CTCTTC 7 cut(s) 19, 62, 272, 332, 374, 741, 1185
Eco130I CCWWGG 1 cut(s) 1146
Eco24I GRGCYC 2 cut(s) 241, 286
Eco31I GGTCTC 1 cut(s) 1187
Eco47I GGWCC 1 cut(s) 1129
Eco57I CTGAAG 1 cut(s) 1043
Eco81I CCTNAGG 1 cut(s) 1419
EcoT14I CCWWGG 1 cut(s) 1146
EcoT38I GRGCYC 2 cut(s) 241, 286
ErhI CCWWGG 1 cut(s) 1146
Esp3I CGTCTC 1 cut(s) 1001
FaeI CATG 4 cut(s) 842, 940, 953, 1624
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 1 cut(s) 580
FatI CATG 4 cut(s) 838, 936, 949, 1620
FauNDI CATATG 1 cut(s) 757
FblI GTMKAC 1 cut(s) 1326
Fnu4HI GCNGC 3 cut(s) 304, 451, 1344
FriOI GRGCYC 2 cut(s) 241, 286
Fsp4HI GCNGC 3 cut(s) 304, 451, 1344
FspBI CTAG 5 cut(s) 417, 423, 591, 921, 1511
GluI GCNGC 3 cut(s) 304, 451, 1344
GsuI CTGGAG 3 cut(s) 1025, 1191, 1575
HaeIII GGCC 2 cut(s) 5, 698
HapII CCGG 2 cut(s) 654, 1110
Hin1II CATG 4 cut(s) 842, 940, 953, 1624
HindIII AAGCTT 1 cut(s) 184
HpaII CCGG 2 cut(s) 654, 1110
HphI GGTGA 2 cut(s) 494, 865
Hpy166II GTNNAC 6 cut(s) 245, 1327, 1367, 1574, 1601, 1669
Hpy8I GTNNAC 6 cut(s) 245, 1327, 1367, 1574, 1601, 1669
Hpy99I CGWCG 2 cut(s) 141, 144
HpyCH4III ACNGT 6 cut(s) 224, 611, 797, 1483, 1526, 1605
HpyCH4V TGCA 5 cut(s) 607, 905, 1073, 1115, 1346
HpyF10VI GCNNNNNNNGC 1 cut(s) 1711
HpyF3I CTNAG 6 cut(s) 51, 405, 570, 1419, 1440, 1590
Hsp92II CATG 4 cut(s) 842, 940, 953, 1624
Kzo9I GATC 9 cut(s) 122, 293, 587, 680, 760, 946, 966, 1000, 1011
LmnI GCTCC 4 cut(s) 384, 447, 810, 1546
Lsp1109I GCAGC 3 cut(s) 315, 437, 1330
LweI GCATC 4 cut(s) 361, 892, 1495, 1620
MaeI CTAG 5 cut(s) 417, 423, 591, 921, 1511
MaeIII GTNAC 5 cut(s) 482, 649, 1075, 1435, 1483
MalI GATC 9 cut(s) 124, 295, 589, 682, 762, 948, 968, 1002, 1013
MboI GATC 9 cut(s) 122, 293, 587, 680, 760, 946, 966, 1000, 1011
MflI RGATCY 3 cut(s) 122, 587, 966
MhlI GDGCHC 3 cut(s) 241, 286, 741
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 912, 916, 1124
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 329, 449, 604, 1133
MmeI TCCRAC 3 cut(s) 233, 804, 1292
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 916
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 360, 915, 954, 1016
MslI CAYNNNNRTG 3 cut(s) 837, 954, 1625
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 1015
MspI CCGG 2 cut(s) 654, 1110
MvnI CGCG 2 cut(s) 36, 144
MwoI GCNNNNNNNGC 1 cut(s) 1711
NdeI CATATG 1 cut(s) 757
NdeII GATC 9 cut(s) 122, 293, 587, 680, 760, 946, 966, 1000, 1011
NlaIII CATG 4 cut(s) 842, 940, 953, 1624
NlaIV GGNNCC 4 cut(s) 386, 934, 1413, 1706
NmuCI GTSAC 3 cut(s) 482, 649, 1483
NruI TCGCGA 1 cut(s) 144
NspI RCATGY 1 cut(s) 842
PciI ACATGT 1 cut(s) 838
PdmI GAANNNNTTC 1 cut(s) 916
PfeI GAWTC 6 cut(s) 149, 176, 197, 494, 637, 1217
PflFI GACNNNGTC 1 cut(s) 1621
PkrI GCNGC 3 cut(s) 305, 452, 1345
PleI GAGTC 4 cut(s) 328, 448, 603, 1133
PpsI GAGTC 4 cut(s) 328, 448, 603, 1133
PscI ACATGT 1 cut(s) 838
PshBI ATTAAT 1 cut(s) 915
PspN4I GGNNCC 4 cut(s) 386, 934, 1413, 1706
PspPI GGNCC 1 cut(s) 1129
PstNI CAGNNNCTG 2 cut(s) 1079, 1466
PsuI RGATCY 3 cut(s) 122, 587, 966
PsyI GACNNNGTC 1 cut(s) 1621
RruI TCGCGA 1 cut(s) 144
RsaI GTAC 1 cut(s) 1650
RsaNI GTAC 1 cut(s) 1649
RseI CAYNNNNRTG 3 cut(s) 837, 954, 1625
SaqAI TTAA 4 cut(s) 360, 915, 954, 1016
SatI GCNGC 3 cut(s) 304, 451, 1344
Sau3AI GATC 9 cut(s) 122, 293, 587, 680, 760, 946, 966, 1000, 1011
Sau96I GGNCC 1 cut(s) 1129
SchI GAGTC 4 cut(s) 329, 449, 604, 1133
SduI GDGCHC 3 cut(s) 241, 286, 741
SfaNI GCATC 4 cut(s) 361, 892, 1495, 1620
SinI GGWCC 1 cut(s) 1129
SmiMI CAYNNNNRTG 3 cut(s) 837, 954, 1625
SmlI CTYRAG 3 cut(s) 187, 1015, 1709
SmoI CTYRAG 3 cut(s) 187, 1015, 1709
SpeI ACTAGT 1 cut(s) 422
Sse9I AATT 3 cut(s) 912, 916, 1124
SsiI CCGC 1 cut(s) 692
SspMI CTAG 5 cut(s) 417, 423, 591, 921, 1511
StyI CCWWGG 1 cut(s) 1146
TaaI ACNGT 6 cut(s) 224, 611, 797, 1483, 1526, 1605
TaqI TCGA 3 cut(s) 58, 392, 455
TaqII GACCGA 1 cut(s) 959
TasI AATT 3 cut(s) 912, 916, 1124
TfiI GAWTC 6 cut(s) 149, 176, 197, 494, 637, 1217
Tru1I TTAA 4 cut(s) 360, 915, 954, 1016
Tru9I TTAA 4 cut(s) 360, 915, 954, 1016
TscAI CASTG 1 cut(s) 1215
TseFI GTSAC 3 cut(s) 482, 649, 1483
TseI GCWGC 3 cut(s) 303, 450, 1343
Tsp45I GTSAC 3 cut(s) 482, 649, 1483
TspDTI ATGAA 5 cut(s) 726, 834, 897, 972, 1248
TspGWI ACGGA 1 cut(s) 819
TspRI CASTG 1 cut(s) 1215
Tth111I GACNNNGTC 1 cut(s) 1621
Vha464I CTTAAG 1 cut(s) 1015
VpaK11BI GGWCC 1 cut(s) 1129
VspI ATTAAT 1 cut(s) 915
XbaI TCTAGA 1 cut(s) 1510
XceI RCATGY 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 1326
XmnI GAANNNNTTC 1 cut(s) 916
XspI CTAG 5 cut(s) 417, 423, 591, 921, 1511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.