Rh5BG453800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016451
Physical Location & Seq
Reverse (-)
72774459 .. 72776822
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG453800.1

Sequence Viewer

Length: 399 bp
ATGCAAACTTGGCAAGGTTGTACCCACACAGGTGTGGTTTCCTTATCAGTTTGGTTTTCTCGTGAAACAATCTTTGAAGCCTTGCAAAGGTGGAATGGAAGAGCTCCCTCTGATGGCCTACTCCTGGGCTTCTGTATTGTCTTGATGGGCTTGGCTTGTGTCCCTATTGTGGCTCTCCACTTCTCTCATGTCTTGGAAGAAGATCCAGGTTGCTATTCAAGGGCATCTAACTCTGTTTTTGAGCCTGTGGTAGATGTACAAATGAATGAATTTCATTCAAGCTGTGTGGACTCATGGCTGACAAAATGGGGGACATGCTGCCCCGTGTGCAAGCATGATATGAGAACAAAATCTGTGAATCCTGAGATCAAGAGAAGGACCTGGTTGGACGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.92

Weight (kDa)

5.9

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 197
AcsI RAATTY 1 cut(s) 269
AfaI GTAC 2 cut(s) 22, 258
AfiI CCNNNNNNNGG 4 cut(s) 87, 113, 124, 169
AgsI TTSAA 3 cut(s) 77, 219, 279
AjnI CCWGG 3 cut(s) 123, 205, 380
AleI CACNNNNGTG 2 cut(s) 30, 32
AluBI AGCT 2 cut(s) 104, 282
AluI AGCT 2 cut(s) 104, 282
Alw21I GWGCWC 1 cut(s) 106
AlwI GGATC 1 cut(s) 197
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 318
ApoI RAATTY 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 378
AvaII GGWCC 1 cut(s) 378
BanII GRGCYC 1 cut(s) 106
BauI CACGAG 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 1 cut(s) 305
BccI CCATC 2 cut(s) 107, 139
BciT130I CCWGG 3 cut(s) 125, 207, 382
BisI GCNGC 1 cut(s) 319
BlsI GCNGC 1 cut(s) 320
Bme1390I CCNGG 3 cut(s) 125, 207, 382
Bme18I GGWCC 1 cut(s) 378
BmgT120I GGNCC 1 cut(s) 378
BmrFI CCNGG 3 cut(s) 125, 207, 382
BmsI GCATC 1 cut(s) 233
BsaJI CCNNGG 1 cut(s) 124
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 113, 124, 169
BseBI CCWGG 3 cut(s) 125, 207, 382
BseDI CCNNGG 1 cut(s) 124
BseLI CCNNNNNNNGG 4 cut(s) 87, 113, 124, 169
BseMII CTCAG 1 cut(s) 354
BseXI GCAGC 1 cut(s) 305
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 106
BslFI GGGAC 2 cut(s) 146, 325
BslI CCNNNNNNNGG 4 cut(s) 87, 113, 124, 169
BsmFI GGGAC 2 cut(s) 146, 325
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 106
Bsp1407I TGTACA 1 cut(s) 256
Bsp143I GATC 2 cut(s) 202, 366
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 355
BspHI TCATGA 1 cut(s) 395
BspPI GGATC 1 cut(s) 197
BspQI GCTCTTC 1 cut(s) 94
BsrGI TGTACA 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 2 cut(s) 202, 366
BssSI CACGAG 1 cut(s) 60
Bst2BI CACGAG 1 cut(s) 60
Bst2UI CCWGG 3 cut(s) 125, 207, 382
Bst6I CTCTTC 1 cut(s) 94
BstAUI TGTACA 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 363
BstENI CCTNNNNNAGG 1 cut(s) 85
BstKTI GATC 2 cut(s) 205, 369
BstMBI GATC 2 cut(s) 202, 366
BstMWI GCNNNNNNNGC 2 cut(s) 10, 327
BstNI CCWGG 3 cut(s) 125, 207, 382
BstNSI RCATGY 1 cut(s) 318
BstSCI CCNGG 3 cut(s) 123, 205, 380
BstV1I GCAGC 1 cut(s) 305
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 332
CciI TCATGA 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 378
CsiI ACCWGGT 1 cut(s) 380
Csp6I GTAC 2 cut(s) 21, 257
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviAII CATG 5 cut(s) 188, 294, 315, 335, 396
CviQI GTAC 2 cut(s) 21, 257
DdeI CTNAG 1 cut(s) 363
DpnI GATC 2 cut(s) 204, 368
DpnII GATC 2 cut(s) 202, 366
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Ecl136II GAGCTC 1 cut(s) 104
Eco24I GRGCYC 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 104
EcoNI CCTNNNNNAGG 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 378
EcoRII CCWGG 3 cut(s) 123, 205, 380
EcoT38I GRGCYC 1 cut(s) 106
FaeI CATG 5 cut(s) 191, 297, 318, 338, 399
FaiI YATR 6 cut(s) 189, 295, 316, 336, 341, 397
FaqI GGGAC 2 cut(s) 146, 325
FatI CATG 5 cut(s) 187, 293, 314, 334, 395
Fnu4HI GCNGC 1 cut(s) 319
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 1 cut(s) 319
GluI GCNGC 1 cut(s) 319
HaeIII GGCC 1 cut(s) 117
Hin1II CATG 5 cut(s) 191, 297, 318, 338, 399
HinfI GANTC 3 cut(s) 290, 358, 392
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 5 cut(s) 62, 142, 362, 370, 396
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 1 cut(s) 369
HpyCH4V TGCA 3 cut(s) 4, 85, 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 327
HpyF3I CTNAG 1 cut(s) 363
Hsp92II CATG 5 cut(s) 191, 297, 318, 338, 399
Kzo9I GATC 2 cut(s) 202, 366
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 9 cut(s) 15, 110, 137, 192, 219, 258, 367, 375, 394
Lsp1109I GCAGC 1 cut(s) 305
LweI GCATC 1 cut(s) 233
MabI ACCWGGT 1 cut(s) 380
MalI GATC 2 cut(s) 204, 368
MboI GATC 2 cut(s) 202, 366
MboII GAAGA 3 cut(s) 111, 209, 212
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 1 cut(s) 269
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 1 cut(s) 118
MslI CAYNNNNRTG 2 cut(s) 30, 32
MspR9I CCNGG 3 cut(s) 125, 207, 382
MvaI CCWGG 3 cut(s) 125, 207, 382
MwoI GCNNNNNNNGC 2 cut(s) 10, 327
NdeII GATC 2 cut(s) 202, 366
NlaIII CATG 5 cut(s) 191, 297, 318, 338, 399
NspI RCATGY 1 cut(s) 318
OliI CACNNNNGTG 2 cut(s) 30, 32
PagI TCATGA 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 94
PfeI GAWTC 2 cut(s) 358, 392
PkrI GCNGC 1 cut(s) 320
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
PpuMI RGGWCCY 1 cut(s) 378
Psp124BI GAGCTC 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 378
Psp6I CCWGG 3 cut(s) 123, 205, 380
PspGI CCWGG 3 cut(s) 123, 205, 380
PspPI GGNCC 1 cut(s) 378
PspPPI RGGWCCY 1 cut(s) 378
PsuI RGATCY 1 cut(s) 202
RsaI GTAC 2 cut(s) 22, 258
RsaNI GTAC 2 cut(s) 21, 257
RseI CAYNNNNRTG 2 cut(s) 30, 32
SacI GAGCTC 1 cut(s) 106
SapI GCTCTTC 1 cut(s) 94
SatI GCNGC 1 cut(s) 319
Sau3AI GATC 2 cut(s) 202, 366
Sau96I GGNCC 1 cut(s) 378
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 3 cut(s) 125, 207, 382
SduI GDGCHC 1 cut(s) 106
SetI ASST 7 cut(s) 19, 34, 92, 106, 211, 284, 383
SexAI ACCWGGT 1 cut(s) 380
SfaNI GCATC 1 cut(s) 233
SinI GGWCC 1 cut(s) 378
SmiMI CAYNNNNRTG 2 cut(s) 30, 32
Sse9I AATT 1 cut(s) 269
SstI GAGCTC 1 cut(s) 106
StyD4I CCNGG 3 cut(s) 123, 205, 380
TasI AATT 1 cut(s) 269
TatI WGTACW 1 cut(s) 256
TfiI GAWTC 2 cut(s) 358, 392
TseI GCWGC 1 cut(s) 318
TspDTI ATGAA 4 cut(s) 263, 278, 282, 384
VpaK11BI GGWCC 1 cut(s) 378
XagI CCTNNNNNAGG 1 cut(s) 85
XapI RAATTY 1 cut(s) 269
XceI RCATGY 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.