Rh6AG032400

187-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0016429
Physical Location & Seq
Forward (+)
3989224 .. 4002435
13212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG032400.1

Sequence Viewer

Length: 1176 bp
ATGTCACCTCCCTCGACGACGGTGAGAGCTCTTTTCGATAACTCAAATCTTCACAAATTCAATCAAAGCTTGAATTGTCTCGAAGTTCAATATTTTGAAGCTTTCGATGTTTCTTTTGCAGACGACGAGCTGTCGGCTTCTCCTCCAAGTAAGAAGAGGAAGGAGGCTTCGAATTCAGTAGTAAGAAGCTATTATCCACTATTGGATATTACCGTTTTACATATTATTTTCAATTTGTTGATATTTTTGGATGAATTGGGGATTAGGTTGAAAAGGTTGATGAAAGTGTCAAGGTTACAGTTAGAGGAAGACGACGAGGACTGGTTGCTTCCTCCCTCCAAAGTTACTAACAAGTTGGGTGATGATTCCATCTTAAAGGCCTTGAGGGAATTAAACTATGGTAGTATCCAAATGATGGAGAAGCTTAAATATACACCAGTTCGTTCAGATTCAGTGGTTGGTGAACTGAGGTTGTCTGAGCCAACTGACATTATTCTTCCAGAGCTTCCGAGACTAGAGATGCTTGCTCTCACTGGAAAAGCAATCGAAGGTGATGTTCTTACCGTTGTTGAAGTGATCCCAGAGAGTTTGACTCAACAGCTTGTTTGGCACAAGTACAAGCAAGATGTCAGATATCAATGGTTCTTTTCTTCAGCAGTGGGAGATAATAAGACCTTTGAACCATTACCTGCTCAGCGTTCTTGCTCCTACAAGATGCGATTAGAGGACGTCGGGCGCTGTCTAAAATGTGAGTGCATTGTGACTGATGTGTTTGGAAGGTCAGCTGAGCCTGCATATGCTGAAACTGGACCAATACTACCAGGAATTCCTAGAATTGATAAGCTGGAGATCGAAGGGAGGGGTTTCCACACCAACTTATATGCTGTACGTGGCATTTATAGTGGAGGGAAGGAAGGCAAAAGTAGAATACAGTGGCTCAGATCAATGGTTGGGAGTCCTGATCTTATCTCTATACCCGGGGAGGTAGGGAGAATGTATGAGTCCAATGTGGATGATGTAGGATATAGGCTTGTAGCCATTTACACTCCTGTAAGAGAGGATGGAGTGGAGGGGTCACCTGTTTCAGCATCGACAGAGCCGATTGCAGTCGAGCCTGATGTTCTTAAAGAAGTGAAGCAGAAGCTTGATCTCGGATCAGTGAAATTTGAGGTATGA

Protein Analysis

391

Amino Acids

43.98

Weight (kDa)

5.14

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IG_AIR9 PF23197 279 - 364 7.9e-21 AIR9 A9 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 732
Acc36I ACCTGC 1 cut(s) 697
AccB7I CCANNNNNTGG 1 cut(s) 415
AclWI GGATC 2 cut(s) 571, 1162
AcsI RAATTY 4 cut(s) 56, 172, 825, 1163
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 1 cut(s) 729
AfaI GTAC 2 cut(s) 617, 888
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 8 cut(s) 61, 73, 89, 98, 232, 271, 572, 680
AhdI GACNNNNNGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 820
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 2 cut(s) 83, 505
AlwI GGATC 2 cut(s) 571, 1162
Ama87I CYCGRG 1 cut(s) 977
AoxI GGCC 1 cut(s) 378
ApoI RAATTY 4 cut(s) 56, 172, 825, 1163
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 1 cut(s) 809
AsuC2I CCSGG 2 cut(s) 978, 979
AsuHPI GGTGA 5 cut(s) 34, 371, 473, 563, 1068
AsuII TTCGAA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 977
AvaII GGWCC 1 cut(s) 809
BanII GRGCYC 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 31
BccI CCATC 3 cut(s) 377, 409, 1055
BciT130I CCWGG 1 cut(s) 822
BciVI GTATCC 1 cut(s) 416
BcnI CCSGG 2 cut(s) 978, 979
BcoDI GTCTC 2 cut(s) 83, 505
BfaI CTAG 2 cut(s) 515, 831
BfoI RGCGCY 1 cut(s) 739
BfuAI ACCTGC 1 cut(s) 697
BfuI GTATCC 1 cut(s) 416
BlpI GCTNAGC 2 cut(s) 693, 786
Bme1390I CCNGG 3 cut(s) 822, 978, 979
Bme18I GGWCC 1 cut(s) 809
BmeRI GACNNNNNGTC 1 cut(s) 130
BmeT110I CYCGRG 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 809
BmrFI CCNGG 3 cut(s) 822, 978, 979
BmsI GCATC 3 cut(s) 510, 705, 1097
BpiI GAAGAC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 577, 609
BpmI CTGGAG 1 cut(s) 866
Bpu1102I GCTNAGC 2 cut(s) 693, 786
Bpu14I TTCGAA 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 403
BpuMI CCSGG 2 cut(s) 978, 979
BsaAI YACGTR 1 cut(s) 890
BsaHI GRCGYC 1 cut(s) 729
BsaJI CCNNGG 2 cut(s) 977, 978
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 4 cut(s) 326, 437, 538, 811
BseBI CCWGG 1 cut(s) 822
BseDI CCNNGG 2 cut(s) 977, 978
BseGI GGATG 3 cut(s) 256, 1018, 1066
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMII CTCAG 5 cut(s) 458, 468, 707, 777, 952
BseNI ACTGG 4 cut(s) 326, 437, 538, 811
BseRI GAGGAG 1 cut(s) 132
BshFI GGCC 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 977
BsiSI CCGG 1 cut(s) 978
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 2 cut(s) 83, 505
BsnI GGCC 1 cut(s) 380
BsoBI CYCGRG 1 cut(s) 977
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 6 cut(s) 576, 849, 941, 961, 1147, 1154
Bsp1720I GCTNAGC 2 cut(s) 693, 786
BspANI GGCC 1 cut(s) 380
BspCNI CTCAG 5 cut(s) 459, 469, 706, 778, 951
BspMI ACCTGC 1 cut(s) 697
BspPI GGATC 2 cut(s) 571, 1162
BspT104I TTCGAA 1 cut(s) 170
BsrI ACTGG 4 cut(s) 326, 437, 538, 811
BssECI CCNNGG 2 cut(s) 977, 978
BssMI GATC 6 cut(s) 576, 849, 941, 961, 1147, 1154
BssNI GRCGYC 1 cut(s) 729
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 5 cut(s) 22, 214, 300, 565, 933
Bst6I CTCTTC 1 cut(s) 149
BstACI GRCGYC 1 cut(s) 729
BstBAI YACGTR 1 cut(s) 890
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 525, 792
BstDEI CTNAG 5 cut(s) 467, 477, 693, 786, 938
BstEII GGTNACC 1 cut(s) 1074
BstF5I GGATG 3 cut(s) 256, 1018, 1066
BstH2I RGCGCY 1 cut(s) 739
BstHHI GCGC 1 cut(s) 738
BstKTI GATC 6 cut(s) 579, 852, 944, 964, 1150, 1157
BstMAI GTCTC 2 cut(s) 83, 505
BstMBI GATC 6 cut(s) 576, 849, 941, 961, 1147, 1154
BstMWI GCNNNNNNNGC 2 cut(s) 607, 791
BstNI CCWGG 1 cut(s) 822
BstPI GGTNACC 1 cut(s) 1074
BstSCI CCNGG 3 cut(s) 820, 976, 977
BstV2I GAAGAC 1 cut(s) 315
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 1 cut(s) 380
BtsCI GGATG 3 cut(s) 256, 1018, 1066
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 5 cut(s) 459, 531, 663, 938, 1164
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 525, 792
CfoI GCGC 1 cut(s) 738
Cfr13I GGNCC 1 cut(s) 809
Cfr9I CCCGGG 1 cut(s) 977
Csp6I GTAC 2 cut(s) 616, 887
CviQI GTAC 2 cut(s) 616, 887
DdeI CTNAG 5 cut(s) 467, 477, 693, 786, 938
DpnI GATC 6 cut(s) 578, 851, 943, 963, 1149, 1156
DpnII GATC 6 cut(s) 576, 849, 941, 961, 1147, 1154
DriI GACNNNNNGTC 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 149
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 29
Eco147I AGGCCT 1 cut(s) 380
Eco24I GRGCYC 1 cut(s) 31
Eco32I GATATC 1 cut(s) 635
Eco47I GGWCC 1 cut(s) 809
Eco53kI GAGCTC 1 cut(s) 29
Eco57I CTGAAG 1 cut(s) 636
Eco88I CYCGRG 1 cut(s) 977
Eco91I GGTNACC 1 cut(s) 1074
EcoICRI GAGCTC 1 cut(s) 29
EcoO65I GGTNACC 1 cut(s) 1074
EcoRI GAATTC 2 cut(s) 172, 825
EcoRII CCWGG 1 cut(s) 820
EcoRV GATATC 1 cut(s) 635
EcoT38I GRGCYC 1 cut(s) 31
FauNDI CATATG 1 cut(s) 796
FokI GGATG 3 cut(s) 263, 1025, 1073
FriOI GRGCYC 1 cut(s) 31
FspBI CTAG 2 cut(s) 515, 831
GlaI GCGC 1 cut(s) 737
GsuI CTGGAG 1 cut(s) 866
HaeII RGCGCY 1 cut(s) 739
HaeIII GGCC 1 cut(s) 380
HapII CCGG 1 cut(s) 978
HhaI GCGC 1 cut(s) 738
Hin1I GRCGYC 1 cut(s) 729
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HindIII AAGCTT 4 cut(s) 67, 99, 422, 1142
HinfI GANTC 5 cut(s) 365, 449, 592, 955, 1001
HpaII CCGG 1 cut(s) 978
HphI GGTGA 5 cut(s) 34, 371, 473, 563, 1068
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 6 cut(s) 448, 478, 510, 632, 941, 1154
Hpy188III TCNNGA 3 cut(s) 80, 500, 959
Hpy8I GTNNAC 1 cut(s) 464
Hpy99I CGWCG 5 cut(s) 19, 22, 128, 317, 734
HpyAV CCTTC 6 cut(s) 154, 542, 771, 848, 904, 908
HpyCH4III ACNGT 5 cut(s) 22, 214, 300, 565, 933
HpyCH4IV ACGT 2 cut(s) 729, 889
HpyCH4V TGCA 4 cut(s) 119, 756, 794, 1106
HpyF10VI GCNNNNNNNGC 2 cut(s) 607, 791
HpyF3I CTNAG 5 cut(s) 467, 477, 693, 786, 938
HpySE526I ACGT 2 cut(s) 729, 889
Hsp92I GRCGYC 1 cut(s) 729
HspAI GCGC 1 cut(s) 736
Kzo9I GATC 6 cut(s) 576, 849, 941, 961, 1147, 1154
LmnI GCTCC 1 cut(s) 710
LweI GCATC 3 cut(s) 510, 705, 1097
MaeI CTAG 2 cut(s) 515, 831
MaeII ACGT 2 cut(s) 729, 889
MaeIII GTNAC 5 cut(s) 3, 294, 343, 760, 1074
MalI GATC 6 cut(s) 578, 851, 943, 963, 1149, 1156
MboI GATC 6 cut(s) 576, 849, 941, 961, 1147, 1154
MboII GAAGA 5 cut(s) 41, 166, 320, 488, 642
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 9 cut(s) 56, 73, 172, 232, 254, 389, 825, 834, 1163
MlyI GAGTC 3 cut(s) 586, 964, 1010
MseI TTAA 4 cut(s) 374, 392, 426, 1125
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 1 cut(s) 978
MspR9I CCNGG 3 cut(s) 822, 978, 979
MvaI CCWGG 1 cut(s) 822
MwoI GCNNNNNNNGC 2 cut(s) 607, 791
NciI CCSGG 2 cut(s) 978, 979
NdeI CATATG 1 cut(s) 796
NdeII GATC 6 cut(s) 576, 849, 941, 961, 1147, 1154
NmuCI GTSAC 3 cut(s) 3, 760, 1074
NspV TTCGAA 1 cut(s) 170
PceI AGGCCT 1 cut(s) 380
PcsI WCGNNNNNNNCGW 1 cut(s) 1097
PfeI GAWTC 2 cut(s) 365, 449
PflMI CCANNNNNTGG 1 cut(s) 415
PleI GAGTC 3 cut(s) 586, 963, 1009
PpsI GAGTC 3 cut(s) 586, 963, 1009
Ppu21I YACGTR 1 cut(s) 890
Psp124BI GAGCTC 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 820
PspEI GGTNACC 1 cut(s) 1074
PspGI CCWGG 1 cut(s) 820
PspPI GGNCC 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 2 cut(s) 617, 888
RsaNI GTAC 2 cut(s) 616, 887
SacI GAGCTC 1 cut(s) 31
SaqAI TTAA 4 cut(s) 374, 392, 426, 1125
Sau3AI GATC 6 cut(s) 576, 849, 941, 961, 1147, 1154
Sau96I GGNCC 1 cut(s) 809
SchI GAGTC 3 cut(s) 586, 964, 1010
ScrFI CCNGG 3 cut(s) 822, 978, 979
SduI GDGCHC 1 cut(s) 31
SfaNI GCATC 3 cut(s) 510, 705, 1097
SfuI TTCGAA 1 cut(s) 170
SinI GGWCC 1 cut(s) 809
SmaI CCCGGG 1 cut(s) 979
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 9 cut(s) 56, 73, 172, 232, 254, 389, 825, 834, 1163
SseBI AGGCCT 1 cut(s) 380
SspI AATATT 1 cut(s) 92
SspMI CTAG 2 cut(s) 515, 831
SstI GAGCTC 1 cut(s) 31
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 820, 976, 977
TaaI ACNGT 5 cut(s) 22, 214, 300, 565, 933
TaiI ACGT 2 cut(s) 732, 892
TaqI TCGA 9 cut(s) 14, 36, 81, 105, 170, 546, 852, 1091, 1110
TasI AATT 9 cut(s) 56, 73, 172, 232, 254, 389, 825, 834, 1163
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 2 cut(s) 365, 449
Tru1I TTAA 4 cut(s) 374, 392, 426, 1125
Tru9I TTAA 4 cut(s) 374, 392, 426, 1125
TscAI CASTG 5 cut(s) 459, 538, 663, 938, 1164
TseFI GTSAC 3 cut(s) 3, 760, 1074
Tsp45I GTSAC 3 cut(s) 3, 760, 1074
TspDTI ATGAA 2 cut(s) 267, 296
TspMI CCCGGG 1 cut(s) 977
TspRI CASTG 5 cut(s) 459, 538, 663, 938, 1164
Van91I CCANNNNNTGG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 809
XapI RAATTY 4 cut(s) 56, 172, 825, 1163
XmaI CCCGGG 1 cut(s) 977
XspI CTAG 2 cut(s) 515, 831
ZraI GACGTC 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.