Rh6BG295000

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005214
Physical Location & Seq
Reverse (-)
52355977 .. 52357993
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG295000.1

Sequence Viewer

Length: 642 bp
ATGAACAGCAAAGAGGTCATCTTTCATGTCCACAGAATGAGCAAGCACAACAGAGAGGAGGTTCTGCAGAAAGCCGAAAAGGATTTAGCCGCCGTGACGGATCAGTTCGTGGCCCGGCTGGTTAAAGAGCTGCAAGGGACTGTTTTTTGGAAGCTACGACGAGCATACTCGCCTGGGTTTTACTCGATTTGGCCACCAAGACAGCCAACATGGGTGGGAATGTTATCACTGGTATCTGGTCTCACAACCCAGAGGAATGGCCTATTAGACAACTCAAGAGCTAAAGCCAGATTTTCTGAGTGCTTGAATTTCCTGTACACGCCCACTGAGAATTTCACAGTGGACTATTGGAATACTGGGAGCAGTATTATAGATAGTACCCACTGCTGGATTCTCAAAAGGCCGCACTACAATGTCAATATGAAGGCAGTTCAAGTCGGCCATGCTTTCCTAAATATTTCTACAGATGTATTTGAAGCGGGAGATAGAAAAGGGACTATAATTGACAGCGGTACAACTTTGGCCTATCTTCCAGACATCGTGTATGAGCCCTTAGTAAGTCAGATCTTCTCTCAGCAGCCTGATATGAAAATTCAGACTCTTCGTGGCGAGTATACATGCTTTGAGTACTCGGGAAGGTAA

Protein Analysis

213

Amino Acids

24.48

Weight (kDa)

8.72

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_C PF14541 136 - 209 9.6e-07 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 614
AciI CCGC 4 cut(s) 90, 404, 479, 510
AclWI GGATC 1 cut(s) 108
AcoI YGGCCR 2 cut(s) 191, 439
AcsI RAATTY 3 cut(s) 307, 331, 591
AfaI GTAC 4 cut(s) 317, 379, 514, 629
AfiI CCNNNNNNNGG 1 cut(s) 387
AgsI TTSAA 3 cut(s) 307, 434, 476
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 3 cut(s) 130, 154, 281
AluI AGCT 3 cut(s) 130, 154, 281
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 1 cut(s) 108
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 6 cut(s) 111, 191, 259, 401, 439, 522
ApeKI GCWGC 2 cut(s) 130, 577
ApoI RAATTY 3 cut(s) 307, 331, 591
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 631
BalI TGGCCA 1 cut(s) 193
BanII GRGCYC 1 cut(s) 552
BbvI GCAGC 2 cut(s) 117, 589
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 174
BcnI CCSGG 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 245
BfmI CTRYAG 2 cut(s) 65, 462
BglII AGATCT 1 cut(s) 564
BisI GCNGC 4 cut(s) 90, 131, 404, 578
BlsI GCNGC 4 cut(s) 91, 132, 405, 579
BmcAI AGTACT 1 cut(s) 629
Bme1390I CCNGG 2 cut(s) 115, 174
BmeT110I CYCGRG 1 cut(s) 631
BmgT120I GGNCC 1 cut(s) 112
BmrFI CCNGG 2 cut(s) 115, 174
BmrI ACTGGG 1 cut(s) 366
BmuI ACTGGG 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 115
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 173
Bsc4I CCNNNNNNNGG 1 cut(s) 387
Bse1I ACTGG 2 cut(s) 234, 361
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 387
BseMII CTCAG 3 cut(s) 288, 318, 587
BseNI ACTGG 2 cut(s) 234, 361
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 117, 589
BshFI GGCC 6 cut(s) 113, 193, 261, 403, 441, 524
BsiHKCI CYCGRG 1 cut(s) 631
BsiSI CCGG 1 cut(s) 115
BslFI GGGAC 2 cut(s) 151, 508
BslI CCNNNNNNNGG 1 cut(s) 387
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 2 cut(s) 151, 508
BsnI GGCC 6 cut(s) 113, 193, 261, 403, 441, 524
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 552
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 2 cut(s) 100, 564
BspACI CCGC 4 cut(s) 90, 404, 479, 510
BspANI GGCC 6 cut(s) 113, 193, 261, 403, 441, 524
BspCNI CTCAG 3 cut(s) 289, 319, 586
BspMAI CTGCAG 1 cut(s) 69
BspPI GGATC 1 cut(s) 108
BspTNI GGTCTC 1 cut(s) 245
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 2 cut(s) 234, 361
BssECI CCNNGG 1 cut(s) 173
BssMI GATC 2 cut(s) 100, 564
BssNAI GTATAC 1 cut(s) 615
Bst1107I GTATAC 1 cut(s) 615
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 2 cut(s) 142, 340
Bst6I CTCTTC 1 cut(s) 606
BstAUI TGTACA 1 cut(s) 315
BstC8I GCNNGC 1 cut(s) 44
BstDEI CTNAG 4 cut(s) 297, 327, 553, 573
BstKTI GATC 2 cut(s) 103, 567
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 100, 564
BstNI CCWGG 1 cut(s) 174
BstNSI RCATGY 1 cut(s) 621
BstSCI CCNGG 2 cut(s) 113, 172
BstSFI CTRYAG 2 cut(s) 65, 462
BstV1I GCAGC 2 cut(s) 117, 589
BstX2I RGATCY 1 cut(s) 564
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 564
BstZ17I GTATAC 1 cut(s) 615
BsuRI GGCC 6 cut(s) 113, 193, 261, 403, 441, 524
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 4 cut(s) 227, 324, 345, 382
Cac8I GCNNGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 112
Csp6I GTAC 4 cut(s) 316, 378, 513, 628
CspCI CAANNNNNGTGG 2 cut(s) 195, 230
CviAII CATG 4 cut(s) 26, 210, 443, 618
CviQI GTAC 4 cut(s) 316, 378, 513, 628
DdeI CTNAG 4 cut(s) 297, 327, 553, 573
DpnI GATC 2 cut(s) 102, 566
DpnII GATC 2 cut(s) 100, 564
EaeI YGGCCR 2 cut(s) 191, 439
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 552
Eco31I GGTCTC 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 631
EcoRII CCWGG 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 552
FaeI CATG 4 cut(s) 29, 213, 446, 621
FaqI GGGAC 2 cut(s) 151, 508
FatI CATG 4 cut(s) 25, 209, 442, 617
FauI CCCGC 1 cut(s) 472
FblI GTMKAC 1 cut(s) 614
Fnu4HI GCNGC 4 cut(s) 90, 131, 404, 578
FriOI GRGCYC 1 cut(s) 552
Fsp4HI GCNGC 4 cut(s) 90, 131, 404, 578
GluI GCNGC 4 cut(s) 90, 131, 404, 578
HaeIII GGCC 6 cut(s) 113, 193, 261, 403, 441, 524
HapII CCGG 1 cut(s) 115
Hin1II CATG 4 cut(s) 29, 213, 446, 621
HinfI GANTC 2 cut(s) 391, 598
HpaII CCGG 1 cut(s) 115
Hpy166II GTNNAC 4 cut(s) 31, 318, 343, 615
Hpy188I TCNGA 3 cut(s) 298, 564, 597
Hpy188III TCNNGA 3 cut(s) 276, 533, 633
Hpy8I GTNNAC 4 cut(s) 31, 318, 343, 615
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 2 cut(s) 418, 630
HpyCH4III ACNGT 2 cut(s) 142, 340
HpyCH4V TGCA 2 cut(s) 67, 133
HpyF3I CTNAG 4 cut(s) 297, 327, 553, 573
Hsp92II CATG 4 cut(s) 29, 213, 446, 621
Kzo9I GATC 2 cut(s) 100, 564
LmnI GCTCC 1 cut(s) 360
Lsp1109I GCAGC 2 cut(s) 117, 589
MaeIII GTNAC 1 cut(s) 94
MalI GATC 2 cut(s) 102, 566
MboI GATC 2 cut(s) 100, 564
MboII GAAGA 3 cut(s) 521, 559, 593
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 552
MlsI TGGCCA 1 cut(s) 193
MluCI AATT 4 cut(s) 307, 331, 501, 591
MluNI TGGCCA 1 cut(s) 193
MlyI GAGTC 1 cut(s) 592
MnlI CCTC 4 cut(s) 7, 49, 52, 246
Mox20I TGGCCA 1 cut(s) 193
MscI TGGCCA 1 cut(s) 193
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 411
Msp20I TGGCCA 1 cut(s) 193
MspA1I CMGCKG 1 cut(s) 510
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 115, 174
MvaI CCWGG 1 cut(s) 174
NciI CCSGG 1 cut(s) 115
NdeII GATC 2 cut(s) 100, 564
NlaIII CATG 4 cut(s) 29, 213, 446, 621
NmuCI GTSAC 1 cut(s) 94
NspI RCATGY 1 cut(s) 621
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 4 cut(s) 91, 132, 405, 579
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
Psp6I CCWGG 1 cut(s) 172
PspGI CCWGG 1 cut(s) 172
PspPI GGNCC 1 cut(s) 112
PstI CTGCAG 1 cut(s) 69
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 4 cut(s) 317, 379, 514, 629
RsaNI GTAC 4 cut(s) 316, 378, 513, 628
RseI CAYNNNNRTG 1 cut(s) 411
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 4 cut(s) 90, 131, 404, 578
Sau3AI GATC 2 cut(s) 100, 564
Sau96I GGNCC 1 cut(s) 112
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 2 cut(s) 115, 174
SduI GDGCHC 1 cut(s) 552
SetI ASST 6 cut(s) 18, 63, 132, 156, 283, 641
SfcI CTRYAG 2 cut(s) 65, 462
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 4 cut(s) 307, 331, 501, 591
SsiI CCGC 4 cut(s) 90, 404, 479, 510
SspI AATATT 1 cut(s) 457
StyD4I CCNGG 2 cut(s) 113, 172
TaaI ACNGT 2 cut(s) 142, 340
TaqI TCGA 1 cut(s) 185
TasI AATT 4 cut(s) 307, 331, 501, 591
TatI WGTACW 2 cut(s) 315, 627
TauI GCSGC 2 cut(s) 92, 406
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 4 cut(s) 234, 331, 345, 389
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 130, 577
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 4 cut(s) 14, 17, 437, 602
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 4 cut(s) 234, 331, 345, 389
XapI RAATTY 3 cut(s) 307, 331, 591
XceI RCATGY 1 cut(s) 621
XmiI GTMKAC 1 cut(s) 614
ZrmI AGTACT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.