Rh6BG513900

Cell division control protein 48 homolog C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007658
Physical Location & Seq
Reverse (-)
70881387 .. 70886002
4616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG513900.1

Sequence Viewer

Length: 2460 bp
ATGAAGCCGGGAAAGAGGTCTGGCGGAGGAGGAGGGAGGTCGTCGTTTTCCGATAGAAGGCTGAACGAGTTTCGGACTCGGCTGGAGTCGTCCACCGAGTTAGACCACAGGTCCTCCGTGGACGAAATCGTTGACCATCTCCGCGGCGGTTACCCGGACTACGAGCGACTCAAGCGTCAGGTTTTCAGAAGGTTCGTCTCGCAAACCCTAGCCTCTCCCTCGTTCAAGCACTCGAAGCCCCTACCGGTTATGAGCGGCTCATCGGAAGAAGAAGAAGAAGAGGAGCTGGCCGGAAGTAAGAGGCGGAAGCGTGCTCGGAATGAGAGGGAAGAGAGACTGCAGCAGATAGAGGAAGCGCACGTTCGGAACATTAGGCAGAGCAATGGTAATGTGCCGTCTTCATCGTCGTCGTCATCGTCAGCTTCGGAGGACGAAGGAGATGAGGAGTCTACGTCGGAGGACGCGATATACGGCGAGGAATTGGAGCCGAAGTTTGATTTGATGAAATCGATGCTGCGGGAGGCGTATACGGATCCGAAAAGCGGCGGGAAATCCAAGGCGGCGGAGCAGCAGAAGGAGAAGAATGTGGAAATGGATATTCCAGGGCGGGAGAAGGTTAAATTAGTGGCCGGAAGTAGAGGGGCGAGGCCGGAGACTGTGTCGAATAAGGCCGAGAGTGAGGCCAAGAGCTCTATTTCGAGAGGTGTTGAGGTGAAGAGGAATGAGGGGCCGAAGTTTAGTGACTTAGGTGGAATGCAGAAAGTGCTGGAGGAGCTGAACAAGGAGGTTATTAGGCCGCTTCGCCGGTCGTGGCTGCCGCAGTGGCTCGGAGTTAGGCCAATGTCTGGGATTTTGTTGTGTGGACCGCCGGGGTGTGGGAAGACCACATTGGCTCATGCCATTGCCAATGAGACTGGCATTCCCTTCTATAAGATTTCGGCCACTGAAGTTGTCTCTGGTGTTTCAGGTGCCTCTGAAGAAAACATACGAGATCTTTTTTCAAAAGCATATAGAACTGCCCCTTCAATTGTTTTTATTGATGAAATTGATGCAATTGCTTCGAAAAGAGAAAATATGCAAAGGGAAATGGAGAAGCGAATGGTTACACAACTGATGACTTGCATGGATCAATCTCATAGACCAGTACAGGCGGAAGATGCAAATTCTGACTCACAAAGTTCAGATCAGGCATCTGGCTATGTTCTTGTTATTGGAGCCACGAATAGGCCTGATGCTGTTGACCCTGCTTTAAGGAGGCCTGGGCGATTTGGTCGTGAGATTCTTTTGGGTGTTCCGGATGAAAGTTCAAGGCTTGAGATTCTGTCTGTGCTTACTCGCAAGCTAAAACTTGAAGGTTCTTTTGATCTTCTAAAGATTGCTAGGTCCACACCAGGGTATGTTGGAGCTGATTTGGCAGAGCTGGCCGACAGGGCAGGTAACATTGCCATGAACAGGATTTTTGATGAAATTGATTCAACCATTGAGGAAGGCAAGGAAGATTTCTGCCGGCAACCTGAAGAAATGGAAAAATATGCCATCAGTATGGCTGATTTTGAGGAAGCAGTTCAAGTCGTTCAACCTTCGTCAAAAAGAGAGGGATTCTCGGCAATTCCTAATGTGAAATGGGAAAATGTTGGTGGGCTAGATCTCTTGAGGAAGGAATTCGACCGTTACATAGTTAGGCGTATCAAACATCCTGAGAACTATGAGAAATTTGGAGTAAATTCAGAGACAGGTTTCTTGCTGTATGGGCCTCCCGGATGTGGAAAAACACTAATAGCAAAGGCTGTTGCTAATGAAGCAGGAGCCAATTTCATTTATATTAAGGGCCCTGAACTTCTGAATAAATATGTTGGAGAAAGTGAACTGGCAGTCCGGACATTATTTACTCGTGCAAGGACATGCTCACCATGCATACTTTTCTTTGATGAGGTGGATGCTTTGACAACAAGGCGGGGAAAAGAAGGGGGGTGGGTTGTTGAGCGGTTATTGAACCAGTTACTTATAGAGTTAGATGGTGCAGACCAGCGGCGGGGTGTATTTGTTGTTGGTGCCACTAATAGACCTGATGTAATGGACCGTGCCTTTTTACGGCCGGGTAGGTTTGGTAAACTAATTTATGTCGCCCCGCCCAACACAGATGAGCGTGGTTTGATCTTGAAAGCTATAGGAAGGAAGTATCCTGTTGATGCGAGTGTAGATCTGGGTGAGATTGGAAAATGGAAGGCTTGTGAAAATTTTAGCGGAGCTGATCTTGCTGCACTGATGGATGAAGCTGCTATGGCTGCTCTTGAAGAGAAAGAGACATCAGCCGAGAGTTCAGATGCATCTTCGTGGACAATTAAAGAAACTCACTTTCAACCAGCACTGGCAAAAATCATACCATCTCTAACAGAAAGGCAACTTCTATACTATGAGAGATTTGGAGAGAGCCTCAAGGCTCCAAGAAATGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
AAA Nucleolin_bd

Protein Analysis

819

Amino Acids

91.01

Weight (kDa)

5.69

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvB_N PF05496 281 - 366 4.5e-07 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 284 - 430 7.2e-41 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 454 - 492 2.1e-09 AAA+ lid domain
AAA PF00004 581 - 708 1.1e-41 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 732 - 775 3.1e-09 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 1424
AccB1I GGYRCC 2 cut(s) 968, 2051
AccB7I CCANNNNNTGG 1 cut(s) 845
AccBSI CCGCTC 2 cut(s) 255, 1984
AccI GTMKAC 2 cut(s) 449, 527
AccII CGCG 2 cut(s) 144, 464
AccIII TCCGGA 2 cut(s) 1294, 1875
AclWI GGATC 3 cut(s) 527, 540, 1134
AcoI YGGCCR 5 cut(s) 288, 627, 939, 1422, 2093
AcsI RAATTY 5 cut(s) 1162, 1661, 1712, 1723, 2236
AcuI CTGAAG 3 cut(s) 966, 996, 1536
AfaI GTAC 1 cut(s) 1146
AgeI ACCGGT 1 cut(s) 244
AhdI GACNNNNNGTC 1 cut(s) 109
AjnI CCWGG 3 cut(s) 601, 1258, 1390
AjuI GAANNNNNNNTTGG 2 cut(s) 872, 904
AloI GAACNNNNNNTCC 2 cut(s) 1857, 1889
Alw21I GWGCWC 2 cut(s) 316, 692
Alw26I GTCTC 7 cut(s) 202, 328, 647, 905, 958, 1724, 2297
AlwI GGATC 3 cut(s) 527, 540, 1134
Aor13HI TCCGGA 2 cut(s) 1294, 1875
ApaI GGGCCC 1 cut(s) 1832
ApeKI GCWGC 7 cut(s) 340, 514, 568, 814, 2258, 2276, 2285
ApoI RAATTY 5 cut(s) 1162, 1661, 1712, 1723, 2236
AsiGI ACCGGT 1 cut(s) 244
Asp700I GAANNNNTTC 2 cut(s) 1563, 1661
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 8 cut(s) 111, 728, 863, 1383, 1751, 1828, 1829, 2077
AsuC2I CCSGG 5 cut(s) 9, 155, 870, 1758, 2097
AsuHPI GGTGA 3 cut(s) 724, 1899, 2219
AsuII TTCGAA 1 cut(s) 1061
AvaII GGWCC 4 cut(s) 111, 863, 1383, 2077
BaeGI GKGCMC 1 cut(s) 1832
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 2 cut(s) 968, 2051
BanII GRGCYC 2 cut(s) 692, 1832
BarI GAAGNNNNNNTAC 2 cut(s) 969, 1001
BauI CACGAG 1 cut(s) 1890
BbsI GAAGAC 2 cut(s) 390, 887
Bbv12I GWGCWC 2 cut(s) 316, 692
BbvI GCAGC 7 cut(s) 352, 501, 580, 801, 2245, 2263, 2272
BccI CCATC 5 cut(s) 144, 1544, 2009, 2260, 2392
BceAI ACGGC 3 cut(s) 379, 487, 2108
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciT130I CCWGG 3 cut(s) 603, 1260, 1392
BciVI GTATCC 1 cut(s) 2190
BcnI CCSGG 5 cut(s) 9, 155, 870, 1758, 2097
BcoDI GTCTC 7 cut(s) 202, 328, 647, 905, 958, 1724, 2297
BfaI CTAG 3 cut(s) 209, 1380, 1643
BfmI CTRYAG 2 cut(s) 338, 2166
BfuAI ACCTGC 1 cut(s) 1424
BfuI GTATCC 1 cut(s) 2190
BglI GCCNNNNNGGC 2 cut(s) 823, 1430
BglII AGATCT 3 cut(s) 991, 1645, 2200
Bme1390I CCNGG 8 cut(s) 9, 155, 603, 870, 1260, 1392, 1758, 2097
Bme18I GGWCC 4 cut(s) 111, 863, 1383, 2077
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgT120I GGNCC 8 cut(s) 111, 728, 863, 1383, 1751, 1828, 1829, 2077
BmiI GGNNCC 9 cut(s) 486, 534, 729, 970, 1216, 1807, 1830, 2053, 2442
BmrFI CCNGG 8 cut(s) 9, 155, 603, 870, 1260, 1392, 1758, 2097
BmsI GCATC 9 cut(s) 501, 1039, 1147, 1199, 1222, 1927, 2179, 2314, 2336
BpiI GAAGAC 2 cut(s) 390, 887
BplI GAGNNNNNCTC 4 cut(s) 1586, 1618, 2418, 2450
BpmI CTGGAG 2 cut(s) 104, 788
Bpu14I TTCGAA 1 cut(s) 1061
BpuEI CTTGAG 4 cut(s) 155, 1334, 1672, 2420
BpuMI CCSGG 5 cut(s) 9, 155, 870, 1758, 2097
Bsa29I ATCGAT 1 cut(s) 509
BsaJI CCNNGG 7 cut(s) 117, 142, 555, 602, 869, 1259, 1391
BsaWI WCCGGW 3 cut(s) 244, 1294, 1875
BsaXI ACNNNNNCTCC 8 cut(s) 98, 128, 437, 467, 476, 506, 644, 674
Bse118I RCCGGY 3 cut(s) 244, 804, 1506
Bse1I ACTGG 5 cut(s) 919, 1142, 1872, 1996, 2373
Bse3DI GCAATG 3 cut(s) 388, 900, 1440
BseAI TCCGGA 2 cut(s) 1294, 1875
BseBI CCWGG 3 cut(s) 603, 1260, 1392
BseCI ATCGAT 1 cut(s) 509
BseDI CCNNGG 7 cut(s) 117, 142, 555, 602, 869, 1259, 1391
BseGI GGATG 5 cut(s) 1303, 1693, 1766, 1942, 2275
BseMI GCAATG 3 cut(s) 388, 900, 1440
BseMII CTCAG 1 cut(s) 1689
BseNI ACTGG 5 cut(s) 919, 1142, 1872, 1996, 2373
BseRI GAGGAG 5 cut(s) 42, 45, 296, 458, 785
BseSI GKGCMC 1 cut(s) 1832
BseX3I CGGCCG 1 cut(s) 2093
BseXI GCAGC 7 cut(s) 352, 501, 580, 801, 2245, 2263, 2272
BsgI GTGCAG 2 cut(s) 2040, 2244
Bsh1236I CGCG 2 cut(s) 144, 464
Bsh1285I CGRYCG 3 cut(s) 809, 1669, 2096
BshNI GGYRCC 2 cut(s) 968, 2051
BshTI ACCGGT 1 cut(s) 244
BshVI ATCGAT 1 cut(s) 509
BsiEI CGRYCG 3 cut(s) 809, 1669, 2096
BsiHKAI GWGCWC 2 cut(s) 316, 692
BsmAI GTCTC 7 cut(s) 202, 328, 647, 905, 958, 1724, 2297
BsmBI CGTCTC 1 cut(s) 202
BsmI GAATGC 2 cut(s) 759, 918
Bsp119I TTCGAA 1 cut(s) 1061
Bsp120I GGGCCC 1 cut(s) 1828
Bsp1286I GDGCHC 3 cut(s) 316, 692, 1832
Bsp13I TCCGGA 2 cut(s) 1294, 1875
Bsp143I GATC 9 cut(s) 532, 991, 1126, 1183, 1363, 1645, 2154, 2200, 2251
BspCNI CTCAG 1 cut(s) 1690
BspDI ATCGAT 1 cut(s) 509
BspEI TCCGGA 2 cut(s) 1294, 1875
BspFNI CGCG 2 cut(s) 144, 464
BspLI GGNNCC 9 cut(s) 486, 534, 729, 970, 1216, 1807, 1830, 2053, 2442
BspMAI CTGCAG 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 1424
BspPI GGATC 3 cut(s) 527, 540, 1134
BspT104I TTCGAA 1 cut(s) 1061
BspT107I GGYRCC 2 cut(s) 968, 2051
BsrBI CCGCTC 2 cut(s) 255, 1984
BsrDI GCAATG 3 cut(s) 388, 900, 1440
BsrFI RCCGGY 3 cut(s) 244, 804, 1506
BsrI ACTGG 5 cut(s) 919, 1142, 1872, 1996, 2373
BssAI RCCGGY 3 cut(s) 244, 804, 1506
BssECI CCNNGG 7 cut(s) 117, 142, 555, 602, 869, 1259, 1391
BssMI GATC 9 cut(s) 532, 991, 1126, 1183, 1363, 1645, 2154, 2200, 2251
BssNAI GTATAC 1 cut(s) 528
BssSI CACGAG 1 cut(s) 1890
BssT1I CCWWGG 1 cut(s) 555
Bst1107I GTATAC 1 cut(s) 528
Bst2BI CACGAG 1 cut(s) 1890
Bst2UI CCWGG 3 cut(s) 603, 1260, 1392
Bst4CI ACNGT 3 cut(s) 658, 1670, 2081
Bst6I CTCTTC 4 cut(s) 273, 324, 710, 2289
BstAPI GCANNNNNTGC 1 cut(s) 763
BstBI TTCGAA 1 cut(s) 1061
BstC8I GCNNGC 5 cut(s) 288, 312, 1340, 1422, 1508
BstDEI CTNAG 2 cut(s) 745, 1698
BstDSI CCRYGG 2 cut(s) 117, 142
BstEII GGTNACC 1 cut(s) 149
BstF5I GGATG 5 cut(s) 1303, 1693, 1766, 1942, 2275
BstFNI CGCG 2 cut(s) 144, 464
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 9 cut(s) 535, 994, 1129, 1186, 1366, 1648, 2157, 2203, 2254
BstMAI GTCTC 7 cut(s) 202, 328, 647, 905, 958, 1724, 2297
BstMBI GATC 9 cut(s) 532, 991, 1126, 1183, 1363, 1645, 2154, 2200, 2251
BstMCI CGRYCG 3 cut(s) 809, 1669, 2096
BstNI CCWGG 3 cut(s) 603, 1260, 1392
BstNSI RCATGY 1 cut(s) 1905
BstPI GGTNACC 1 cut(s) 149
BstSCI CCNGG 8 cut(s) 7, 153, 601, 868, 1258, 1390, 1756, 2095
BstSFI CTRYAG 2 cut(s) 338, 2166
BstSLI GKGCMC 1 cut(s) 1832
BstUI CGCG 2 cut(s) 144, 464
BstV1I GCAGC 7 cut(s) 352, 501, 580, 801, 2245, 2263, 2272
BstV2I GAAGAC 2 cut(s) 390, 887
BstX2I RGATCY 4 cut(s) 532, 991, 1645, 2200
BstXI CCANNNNNNTGG 1 cut(s) 1543
BstYI RGATCY 4 cut(s) 532, 991, 1645, 2200
BstZ17I GTATAC 1 cut(s) 528
BstZI CGGCCG 1 cut(s) 2093
Bsu15I ATCGAT 1 cut(s) 509
BsuI GTATCC 1 cut(s) 2190
BsuTUI ATCGAT 1 cut(s) 509
BtgI CCRYGG 2 cut(s) 117, 142
BtsCI GGATG 5 cut(s) 1303, 1693, 1766, 1942, 2275
BtsI GCAGTG 1 cut(s) 827
BtsIMutI CAGTG 4 cut(s) 827, 942, 2261, 2366
BveI ACCTGC 1 cut(s) 1424
Cac8I GCNNGC 5 cut(s) 288, 312, 1340, 1422, 1508
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 3 cut(s) 244, 804, 1506
Cfr13I GGNCC 8 cut(s) 111, 728, 863, 1383, 1751, 1828, 1829, 2077
Cfr42I CCGCGG 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 509
CseI GACGC 2 cut(s) 164, 470
Csp6I GTAC 1 cut(s) 1145
CspAI ACCGGT 1 cut(s) 244
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviAII CATG 6 cut(s) 896, 1123, 1447, 1902, 1911, 2457
CviQI GTAC 1 cut(s) 1145
DdeI CTNAG 2 cut(s) 745, 1698
DpnI GATC 9 cut(s) 534, 993, 1128, 1185, 1365, 1647, 2156, 2202, 2253
DpnII GATC 9 cut(s) 532, 991, 1126, 1183, 1363, 1645, 2154, 2200, 2251
DrdI GACNNNNNNGTC 1 cut(s) 174
DriI GACNNNNNGTC 1 cut(s) 109
DseDI GACNNNNNNGTC 1 cut(s) 174
EaeI YGGCCR 5 cut(s) 288, 627, 939, 1422, 2093
EagI CGGCCG 1 cut(s) 2093
Eam1104I CTCTTC 4 cut(s) 273, 324, 710, 2289
Eam1105I GACNNNNNGTC 1 cut(s) 109
EarI CTCTTC 4 cut(s) 273, 324, 710, 2289
EciI GGCGGA 4 cut(s) 39, 319, 578, 1166
Ecl136II GAGCTC 1 cut(s) 690
EclXI CGGCCG 1 cut(s) 2093
Eco130I CCWWGG 1 cut(s) 555
Eco147I AGGCCT 2 cut(s) 1228, 1258
Eco24I GRGCYC 2 cut(s) 692, 1832
Eco47I GGWCC 4 cut(s) 111, 863, 1383, 2077
Eco52I CGGCCG 1 cut(s) 2093
Eco53kI GAGCTC 1 cut(s) 690
Eco57I CTGAAG 3 cut(s) 966, 996, 1536
Eco91I GGTNACC 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 690
EcoO109I RGGNCCY 3 cut(s) 111, 1828, 1829
EcoO65I GGTNACC 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 1661
EcoRII CCWGG 3 cut(s) 601, 1258, 1390
EcoT14I CCWWGG 1 cut(s) 555
EcoT22I ATGCAT 2 cut(s) 1916, 2329
EcoT38I GRGCYC 2 cut(s) 692, 1832
ErhI CCWWGG 1 cut(s) 555
Esp3I CGTCTC 1 cut(s) 202
FaeI CATG 6 cut(s) 899, 1126, 1450, 1905, 1914, 2460
FatI CATG 6 cut(s) 895, 1122, 1446, 1901, 1910, 2456
FauI CCCGC 6 cut(s) 510, 539, 600, 1947, 2025, 2136
FblI GTMKAC 2 cut(s) 449, 527
FokI GGATG 5 cut(s) 1310, 1680, 1773, 1949, 2282
FriOI GRGCYC 2 cut(s) 692, 1832
FspBI CTAG 3 cut(s) 209, 1380, 1643
GlaI GCGC 1 cut(s) 357
GsuI CTGGAG 2 cut(s) 104, 788
HgaI GACGC 2 cut(s) 164, 470
HhaI GCGC 1 cut(s) 358
Hin1II CATG 6 cut(s) 899, 1126, 1450, 1905, 1914, 2460
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HincII GTYRAC 2 cut(s) 133, 1240
HindII GTYRAC 2 cut(s) 133, 1240
HinfI GANTC 9 cut(s) 76, 86, 168, 446, 1169, 1279, 1318, 1472, 1599
HphI GGTGA 3 cut(s) 724, 1899, 2219
Hpy188III TCNNGA 8 cut(s) 699, 1274, 1295, 1651, 1697, 1876, 2158, 2291
Hpy99I CGWCG 4 cut(s) 46, 409, 412, 457
HpyCH4III ACNGT 3 cut(s) 658, 1670, 2081
HpyCH4IV ACGT 2 cut(s) 360, 452
HpyF3I CTNAG 2 cut(s) 745, 1698
HpySE526I ACGT 2 cut(s) 360, 452
Hsp92II CATG 6 cut(s) 899, 1126, 1450, 1905, 1914, 2460
HspAI GCGC 1 cut(s) 356
Kpn2I TCCGGA 2 cut(s) 1294, 1875
KroI GCCGGC 1 cut(s) 1506
KroNI GCCGGC 1 cut(s) 1508
KspI CCGCGG 1 cut(s) 145
Kzo9I GATC 9 cut(s) 532, 991, 1126, 1183, 1363, 1645, 2154, 2200, 2251
LmnI GCTCC 9 cut(s) 283, 484, 565, 772, 1214, 1403, 1805, 2246, 2446
Lsp1109I GCAGC 7 cut(s) 352, 501, 580, 801, 2245, 2263, 2272
LweI GCATC 9 cut(s) 501, 1039, 1147, 1199, 1222, 1927, 2179, 2314, 2336
MaeI CTAG 3 cut(s) 209, 1380, 1643
MaeII ACGT 2 cut(s) 360, 452
MaeIII GTNAC 6 cut(s) 149, 740, 1102, 1436, 1670, 1998
MalI GATC 9 cut(s) 534, 993, 1128, 1185, 1365, 1647, 2156, 2202, 2253
MbiI CCGCTC 2 cut(s) 255, 1984
MboI GATC 9 cut(s) 532, 991, 1126, 1183, 1363, 1645, 2154, 2200, 2251
MfeI CAATTG 2 cut(s) 1026, 1053
MflI RGATCY 4 cut(s) 532, 991, 1645, 2200
MhlI GDGCHC 3 cut(s) 316, 692, 1832
MlyI GAGTC 5 cut(s) 70, 95, 162, 455, 1163
MmeI TCCRAC 3 cut(s) 435, 1381, 1834
Mph1103I ATGCAT 2 cut(s) 1916, 2329
MroI TCCGGA 2 cut(s) 1294, 1875
MroNI GCCGGC 1 cut(s) 1506
MroXI GAANNNNTTC 2 cut(s) 1563, 1661
MseI TTAA 4 cut(s) 618, 1250, 1824, 2343
MslI CAYNNNNRTG 3 cut(s) 1445, 1541, 2332
MspA1I CMGCKG 2 cut(s) 144, 2029
MspR9I CCNGG 8 cut(s) 9, 155, 603, 870, 1260, 1392, 1758, 2097
MunI CAATTG 2 cut(s) 1026, 1053
Mva1269I GAATGC 2 cut(s) 759, 918
MvaI CCWGG 3 cut(s) 603, 1260, 1392
MvnI CGCG 2 cut(s) 144, 464
NaeI GCCGGC 1 cut(s) 1508
NciI CCSGG 5 cut(s) 9, 155, 870, 1758, 2097
NdeII GATC 9 cut(s) 532, 991, 1126, 1183, 1363, 1645, 2154, 2200, 2251
NgoMIV GCCGGC 1 cut(s) 1506
NlaIII CATG 6 cut(s) 899, 1126, 1450, 1905, 1914, 2460
NlaIV GGNNCC 9 cut(s) 486, 534, 729, 970, 1216, 1807, 1830, 2053, 2442
NmeAIII GCCGAG 4 cut(s) 58, 697, 1583, 2338
NmuCI GTSAC 1 cut(s) 740
NsiI ATGCAT 2 cut(s) 1916, 2329
NspI RCATGY 1 cut(s) 1905
NspV TTCGAA 1 cut(s) 1061
PceI AGGCCT 2 cut(s) 1228, 1258
PcsI WCGNNNNNNNCGW 2 cut(s) 413, 461
PctI GAATGC 2 cut(s) 759, 918
PdiI GCCGGC 1 cut(s) 1508
PdmI GAANNNNTTC 2 cut(s) 1563, 1661
PfeI GAWTC 4 cut(s) 1279, 1318, 1472, 1599
PflFI GACNNNGTC 1 cut(s) 658
PflMI CCANNNNNTGG 1 cut(s) 845
PfoI TCCNGGA 1 cut(s) 1756
PinAI ACCGGT 1 cut(s) 244
PleI GAGTC 5 cut(s) 70, 94, 162, 454, 1163
PpsI GAGTC 5 cut(s) 70, 94, 162, 454, 1163
PpuMI RGGWCCY 1 cut(s) 111
Psp124BI GAGCTC 1 cut(s) 692
Psp5II RGGWCCY 1 cut(s) 111
Psp6I CCWGG 3 cut(s) 601, 1258, 1390
PspEI GGTNACC 1 cut(s) 149
PspGI CCWGG 3 cut(s) 601, 1258, 1390
PspN4I GGNNCC 9 cut(s) 486, 534, 729, 970, 1216, 1807, 1830, 2053, 2442
PspOMI GGGCCC 1 cut(s) 1828
PspPI GGNCC 8 cut(s) 111, 728, 863, 1383, 1751, 1828, 1829, 2077
PspPPI RGGWCCY 1 cut(s) 111
PstI CTGCAG 1 cut(s) 342
PsuI RGATCY 4 cut(s) 532, 991, 1645, 2200
PsyI GACNNNGTC 1 cut(s) 658
RsaI GTAC 1 cut(s) 1146
RsaNI GTAC 1 cut(s) 1145
RseI CAYNNNNRTG 3 cut(s) 1445, 1541, 2332
SacI GAGCTC 1 cut(s) 692
SacII CCGCGG 1 cut(s) 145
SaqAI TTAA 4 cut(s) 618, 1250, 1824, 2343
Sau3AI GATC 9 cut(s) 532, 991, 1126, 1183, 1363, 1645, 2154, 2200, 2251
Sau96I GGNCC 8 cut(s) 111, 728, 863, 1383, 1751, 1828, 1829, 2077
SchI GAGTC 5 cut(s) 70, 95, 162, 455, 1163
ScrFI CCNGG 8 cut(s) 9, 155, 603, 870, 1260, 1392, 1758, 2097
SduI GDGCHC 3 cut(s) 316, 692, 1832
SfaNI GCATC 9 cut(s) 501, 1039, 1147, 1199, 1222, 1927, 2179, 2314, 2336
SfcI CTRYAG 2 cut(s) 338, 2166
Sfr303I CCGCGG 1 cut(s) 145
SfuI TTCGAA 1 cut(s) 1061
SgrBI CCGCGG 1 cut(s) 145
SinI GGWCC 4 cut(s) 111, 863, 1383, 2077
SmiMI CAYNNNNRTG 3 cut(s) 1445, 1541, 2332
SmlI CTYRAG 4 cut(s) 170, 1313, 1651, 2435
SmoI CTYRAG 4 cut(s) 170, 1313, 1651, 2435
SseBI AGGCCT 2 cut(s) 1228, 1258
SspMI CTAG 3 cut(s) 209, 1380, 1643
SstI GAGCTC 1 cut(s) 692
StuI AGGCCT 2 cut(s) 1228, 1258
StyD4I CCNGG 8 cut(s) 7, 153, 601, 868, 1258, 1390, 1756, 2095
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 3 cut(s) 658, 1670, 2081
TaiI ACGT 2 cut(s) 363, 455
TaqI TCGA 6 cut(s) 233, 509, 662, 698, 1061, 1665
TatI WGTACW 1 cut(s) 1144
TauI GCSGC 7 cut(s) 147, 258, 546, 563, 799, 820, 2032
TfiI GAWTC 4 cut(s) 1279, 1318, 1472, 1599
Tru1I TTAA 4 cut(s) 618, 1250, 1824, 2343
Tru9I TTAA 4 cut(s) 618, 1250, 1824, 2343
TscAI CASTG 4 cut(s) 827, 949, 2268, 2373
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 7 cut(s) 340, 514, 568, 814, 2258, 2276, 2285
Tsp45I GTSAC 1 cut(s) 740
TspGWI ACGGA 2 cut(s) 106, 545
TspRI CASTG 4 cut(s) 827, 949, 2268, 2373
Tth111I GACNNNGTC 1 cut(s) 658
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 4 cut(s) 111, 863, 1383, 2077
XapI RAATTY 5 cut(s) 1162, 1661, 1712, 1723, 2236
XceI RCATGY 1 cut(s) 1905
XmiI GTMKAC 2 cut(s) 449, 527
XmnI GAANNNNTTC 2 cut(s) 1563, 1661
XspI CTAG 3 cut(s) 209, 1380, 1643
Zsp2I ATGCAT 2 cut(s) 1916, 2329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.