Rh7AG016800

Domain in histone families 1 and 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009974
Physical Location & Seq
Forward (+)
1114492 .. 1116666
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG016800.1

Sequence Viewer

Length: 1515 bp
ATGGACCCACCACCTCCTCCCAATCCCAATCCTTATCCACCGCCGCCCATCCCCACCTCCACCGCCCCCATCTCCGCCGTCCCAACCGCCGTGGAACCTGACAACAACAACAACGCCGCTCATGTAGCTGCCAACCCCACTCATGCTCCTGCCCCTACTTTCAACCACCCTCCTTATGCTGAGATGATATACACAGCTATTGCAACGTTGAAGGAGAGAGACGGGTCGAGCAGGAGAGCTATAGCTAAGTACATCGAGCAGGTCTACCCGAGTCTCCCACCAACTCACTCGGCCTTGTTGACTCGCCATCTCAAACGACTCAAGAACTCTGGTCATCTTGAGATGGTCAAGAAGTCCTACAAGATCCCAGGCCCTCCCAGATCTGACGCTCCTCCTCCTCCTGCAACCGACTCTCCTGCTCCTGCTCCTACCTCTGTTGCCTCTCCCGGGCAGCCACGTGGCCGCGGCCGTCCTCCGAAGCCCAAGCCGGAACAGCAACAGCAGCTTATGCAGCAGCAGCCGCAGTTGCCACAGTTGATTGTTTCCCAACCGCAGCAGCATCAGCATCAGCATCAGCAGCACATTTTACAACAGCCCATTCCGATTCCGCAGCAGCCAATTCCCATTCCTCAACAGCCAATTCCCATCTCTCACCCGCCATTTCCCATTTCTCAACAGTCATTTCCCATTTCTCAACAGCCGATTCCCTTTTCTCAACAGCCATTTTCCATTTCTCAACAGCCGATTTCGGTTTCAATTCCATTTCCAATTCCAATTCCGCAACAGCCAATTCCCATTCCCCAACAACACCCCATTGTCCAGCCCAATTTTCAATCTTTTCCACAGCCCACCCTGCCGCAGCAGAGCTTTCCCGAGCCCAATTCTCAGCCCATGCCCGTGGATGAGCCTAAGAAGCGACCCGGCCGGCCACGCAAAGCTCCGGCAGCCCTTGCCATAGGGCAGAATAGTCCGGTTGTGTCAGCCAAGAGGGGCAGGGGTCGTCCACCGGGCTCTAGGATGTCGAAGAAGAGACCGGGCCGTCCTCCCAAACCCAAGACTGTTTCGAGTGTTTCGGGTTCTAATGGGCTGGTGAAGAGGAGGCCAGGACGTCCCTCCAAAGCTGAGCCCAGGACTGTGTTTATTCCATATGCTACTAATGTGCCTATAATTGGAGCATTTGAACAGACCAATGGGCCGGGTGTAGTAGTCCCACAACCAGCACCAAGGCCCAGAGGTCGGCCCAAGAAGGATAAGTCGGCTGCCGCCTCTTCTGGTGTGGTGGCCGGCAGTGGTAAGCCACGGGGTAGACCGCCTCTGGTTCCGGGGGTGGAGCGGCCCAAGTTAAGTAGGAGTGGGAGGCCCGTGGGCAGGCCCAAGAAGGTAGTCCTCTTCTTTCTAATAATCTTCCAAGTTGTTAATTTTGTAATTGTTTTGTCCCATATTCCCATAATGAGTAAAGTTTTTGAGTTTCAAGAAATTACGCTACATGAACCTAGTGCCGGTTGTACTGTGTAA

Protein Analysis

504

Amino Acids

54.69

Weight (kDa)

11.1

Isoelectric Point (pI)

72.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 56 - 117 3.1e-16 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48620 AT3G18035 AT3G18035
fragaria_vesca FvH4_5g17210
malus_domestica MD06G1230400.v1.1 MD14G1235000.v1.1
prunus_persica Prupe.5G231600_v2.0.a1
pyrus_communis pycom06g20160 pycom14g19600
rosa_chinensis RchiOBHm_Chr1g0337641 RchiOBHm_Chr7g0178581
rosa_laevigata RLG00000005435
rosa_multiflora Rmu_sc0002320.1_g000001
rosa_roxburghii Rroxscaffold_3G00274790
rosa_rugosa Rorug06G0418100
rosa_samantha Rh7AG016800 Rh7BG016300 Rh7CG017000 Rh7DG016900 Rh7DG161800
rosa_wichuraiana Rw7G001340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1038
AatII GACGTC 1 cut(s) 1111
Acc36I ACCTGC 1 cut(s) 250
AccBSI CCGCTC 2 cut(s) 119, 1333
AccI GTMKAC 2 cut(s) 264, 1306
AccII CGCG 1 cut(s) 465
AclI AACGTT 1 cut(s) 206
AclWI GGATC 1 cut(s) 358
AcoI YGGCCR 5 cut(s) 460, 466, 922, 926, 1281
AcvI CACGTG 1 cut(s) 458
AcyI GRCGYC 1 cut(s) 1108
AfaI GTAC 2 cut(s) 251, 1507
AfiI CCNNNNNNNGG 5 cut(s) 447, 1169, 1236, 1368, 1499
AgsI TTSAA 6 cut(s) 163, 211, 756, 833, 1181, 1472
AjnI CCWGG 3 cut(s) 367, 1102, 1127
AluBI AGCT 8 cut(s) 128, 197, 239, 245, 505, 867, 938, 1121
AluI AGCT 8 cut(s) 128, 197, 239, 245, 505, 867, 938, 1121
Alw26I GTCTC 3 cut(s) 213, 278, 1024
AlwI GGATC 1 cut(s) 358
Ama87I CYCGRG 3 cut(s) 268, 446, 872
AspS9I GGNCC 9 cut(s) 4, 371, 1036, 1193, 1227, 1239, 1335, 1359, 1371
AsuC2I CCSGG 7 cut(s) 447, 448, 921, 1008, 1035, 1197, 1323
AsuHPI GGTGA 2 cut(s) 644, 1102
AvaI CYCGRG 3 cut(s) 268, 446, 872
AvaII GGWCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 879, 1013, 1128
BbrPI CACGTG 1 cut(s) 458
BccI CCATC 5 cut(s) 56, 77, 315, 337, 653
BceAI ACGGC 4 cut(s) 62, 74, 453, 1023
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BciT130I CCWGG 3 cut(s) 369, 1104, 1129
BcnI CCSGG 7 cut(s) 447, 448, 921, 1008, 1035, 1197, 1323
BcoDI GTCTC 3 cut(s) 213, 278, 1024
BfaI CTAG 2 cut(s) 1014, 1494
BfmI CTRYAG 1 cut(s) 240
BfuAI ACCTGC 1 cut(s) 250
BglII AGATCT 1 cut(s) 380
BlpI GCTNAGC 1 cut(s) 1122
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 3 cut(s) 268, 446, 872
BmgT120I GGNCC 9 cut(s) 4, 371, 1036, 1193, 1227, 1239, 1335, 1359, 1371
BmiI GGNNCC 3 cut(s) 6, 96, 1320
BmsI GCATC 3 cut(s) 568, 574, 580
Bpu1102I GCTNAGC 1 cut(s) 1122
BpuEI CTTGAG 2 cut(s) 305, 359
BpuMI CCSGG 7 cut(s) 447, 448, 921, 1008, 1035, 1197, 1323
BsaAI YACGTR 1 cut(s) 458
BsaHI GRCGYC 1 cut(s) 1108
BsaI GGTCTC 1 cut(s) 1024
BsaWI WCCGGW 1 cut(s) 970
BsaXI ACNNNNNCTCC 4 cut(s) 130, 160, 397, 427
Bsc4I CCNNNNNNNGG 5 cut(s) 447, 1169, 1236, 1368, 1499
Bse118I RCCGGY 3 cut(s) 924, 1283, 1499
BseBI CCWGG 3 cut(s) 369, 1104, 1129
BseGI GGATG 3 cut(s) 48, 907, 1023
BseLI CCNNNNNNNGG 5 cut(s) 447, 1169, 1236, 1368, 1499
BseMII CTCAG 3 cut(s) 171, 899, 1113
BseRI GAGGAG 5 cut(s) 6, 381, 384, 387, 1111
BseX3I CGGCCG 2 cut(s) 466, 922
Bsh1236I CGCG 1 cut(s) 465
Bsh1285I CGRYCG 2 cut(s) 469, 925
BsiEI CGRYCG 2 cut(s) 469, 925
BsiHKCI CYCGRG 3 cut(s) 268, 446, 872
BslFI GGGAC 4 cut(s) 65, 1095, 1193, 1420
BslI CCNNNNNNNGG 5 cut(s) 447, 1169, 1236, 1368, 1499
BsmAI GTCTC 3 cut(s) 213, 278, 1024
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 4 cut(s) 65, 1095, 1193, 1420
Bso31I GGTCTC 1 cut(s) 1024
BsoBI CYCGRG 3 cut(s) 268, 446, 872
Bsp1286I GDGCHC 3 cut(s) 879, 1013, 1128
Bsp143I GATC 2 cut(s) 363, 380
Bsp1720I GCTNAGC 1 cut(s) 1122
BspCNI CTCAG 3 cut(s) 172, 898, 1114
BspFNI CGCG 1 cut(s) 465
BspLI GGNNCC 3 cut(s) 6, 96, 1320
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 1024
BsrBI CCGCTC 2 cut(s) 119, 1333
BsrFI RCCGGY 3 cut(s) 924, 1283, 1499
BssAI RCCGGY 3 cut(s) 924, 1283, 1499
BssMI GATC 2 cut(s) 363, 380
BssNI GRCGYC 1 cut(s) 1108
BssT1I CCWWGG 1 cut(s) 1223
Bst2UI CCWGG 3 cut(s) 369, 1104, 1129
Bst4CI ACNGT 5 cut(s) 534, 678, 1060, 1135, 1510
Bst6I CTCTTC 4 cut(s) 1022, 1088, 1273, 1394
BstACI GRCGYC 1 cut(s) 1108
BstAPI GCANNNNNTGC 2 cut(s) 508, 950
BstBAI YACGTR 1 cut(s) 458
BstC8I GCNNGC 3 cut(s) 926, 1285, 1370
BstDEI CTNAG 5 cut(s) 180, 246, 885, 909, 1122
BstDSI CCRYGG 5 cut(s) 90, 463, 897, 1298, 1362
BstF5I GGATG 3 cut(s) 48, 907, 1023
BstFNI CGCG 1 cut(s) 465
BstKTI GATC 2 cut(s) 366, 383
BstMAI GTCTC 3 cut(s) 213, 278, 1024
BstMBI GATC 2 cut(s) 363, 380
BstMCI CGRYCG 2 cut(s) 469, 925
BstNI CCWGG 3 cut(s) 369, 1104, 1129
BstSFI CTRYAG 1 cut(s) 240
BstUI CGCG 1 cut(s) 465
BstX2I RGATCY 2 cut(s) 363, 380
BstXI CCANNNNNNTGG 1 cut(s) 898
BstYI RGATCY 2 cut(s) 363, 380
BstZI CGGCCG 2 cut(s) 466, 922
BtgI CCRYGG 5 cut(s) 90, 463, 897, 1298, 1362
BtsCI GGATG 3 cut(s) 48, 907, 1023
BtsI GCAGTG 1 cut(s) 1294
BtsIMutI CAGTG 1 cut(s) 1294
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 3 cut(s) 926, 1285, 1370
Cfr10I RCCGGY 3 cut(s) 924, 1283, 1499
Cfr13I GGNCC 9 cut(s) 4, 371, 1036, 1193, 1227, 1239, 1335, 1359, 1371
Cfr42I CCGCGG 1 cut(s) 466
Cfr9I CCCGGG 1 cut(s) 446
CseI GACGC 1 cut(s) 395
Csp6I GTAC 2 cut(s) 250, 1506
CspCI CAANNNNNGTGG 2 cut(s) 72, 107
CviAII CATG 4 cut(s) 122, 143, 892, 1487
CviQI GTAC 2 cut(s) 250, 1506
DdeI CTNAG 5 cut(s) 180, 246, 885, 909, 1122
DpnI GATC 2 cut(s) 365, 382
DpnII GATC 2 cut(s) 363, 380
DrdI GACNNNNNNGTC 1 cut(s) 1038
DseDI GACNNNNNNGTC 1 cut(s) 1038
EaeI YGGCCR 5 cut(s) 460, 466, 922, 926, 1281
EagI CGGCCG 2 cut(s) 466, 922
Eam1104I CTCTTC 4 cut(s) 1022, 1088, 1273, 1394
EarI CTCTTC 4 cut(s) 1022, 1088, 1273, 1394
EciI GGCGGA 1 cut(s) 64
EclXI CGGCCG 2 cut(s) 466, 922
Eco130I CCWWGG 1 cut(s) 1223
Eco24I GRGCYC 3 cut(s) 879, 1013, 1128
Eco31I GGTCTC 1 cut(s) 1024
Eco47I GGWCC 1 cut(s) 4
Eco52I CGGCCG 2 cut(s) 466, 922
Eco72I CACGTG 1 cut(s) 458
Eco88I CYCGRG 3 cut(s) 268, 446, 872
EcoO109I RGGNCCY 1 cut(s) 371
EcoRII CCWGG 3 cut(s) 367, 1102, 1127
EcoT14I CCWWGG 1 cut(s) 1223
EcoT38I GRGCYC 3 cut(s) 879, 1013, 1128
ErhI CCWWGG 1 cut(s) 1223
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 4 cut(s) 125, 146, 895, 1490
FaqI GGGAC 4 cut(s) 65, 1095, 1193, 1420
FatI CATG 4 cut(s) 121, 142, 891, 1486
FauI CCCGC 1 cut(s) 663
FauNDI CATATG 1 cut(s) 1147
FblI GTMKAC 2 cut(s) 264, 1306
FokI GGATG 3 cut(s) 35, 914, 1030
FriOI GRGCYC 3 cut(s) 879, 1013, 1128
FseI GGCCGGCC 1 cut(s) 928
FspBI CTAG 2 cut(s) 1014, 1494
HgaI GACGC 1 cut(s) 395
Hin1I GRCGYC 1 cut(s) 1108
Hin1II CATG 4 cut(s) 125, 146, 895, 1490
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 6 cut(s) 271, 301, 318, 410, 604, 703
HphI GGTGA 2 cut(s) 644, 1102
Hpy166II GTNNAC 4 cut(s) 265, 300, 1004, 1307
Hpy188I TCNGA 3 cut(s) 385, 477, 603
Hpy188III TCNNGA 5 cut(s) 322, 338, 349, 872, 1472
Hpy8I GTNNAC 4 cut(s) 265, 300, 1004, 1307
HpyAV CCTTC 3 cut(s) 205, 1240, 1372
HpyCH4III ACNGT 5 cut(s) 534, 678, 1060, 1135, 1510
HpyCH4IV ACGT 3 cut(s) 206, 457, 1108
HpyCH4V TGCA 3 cut(s) 203, 404, 511
HpyF3I CTNAG 5 cut(s) 180, 246, 885, 909, 1122
HpySE526I ACGT 3 cut(s) 206, 457, 1108
Hsp92I GRCGYC 1 cut(s) 1108
Hsp92II CATG 4 cut(s) 125, 146, 895, 1490
KroI GCCGGC 2 cut(s) 924, 1283
KroNI GCCGGC 2 cut(s) 926, 1285
KspI CCGCGG 1 cut(s) 466
Kzo9I GATC 2 cut(s) 363, 380
LmnI GCTCC 7 cut(s) 151, 394, 424, 430, 943, 1172, 1330
LweI GCATC 3 cut(s) 568, 574, 580
MaeI CTAG 2 cut(s) 1014, 1494
MaeII ACGT 3 cut(s) 206, 457, 1108
MalI GATC 2 cut(s) 365, 382
MbiI CCGCTC 2 cut(s) 119, 1333
MboI GATC 2 cut(s) 363, 380
MboII GAAGA 6 cut(s) 1036, 1039, 1105, 1260, 1381, 1396
MflI RGATCY 2 cut(s) 363, 380
MhlI GDGCHC 3 cut(s) 879, 1013, 1128
MlyI GAGTC 4 cut(s) 280, 295, 312, 404
MroNI GCCGGC 2 cut(s) 924, 1283
MseI TTAA 2 cut(s) 1343, 1416
MslI CAYNNNNRTG 1 cut(s) 896
MspA1I CMGCKG 1 cut(s) 465
MvaI CCWGG 3 cut(s) 369, 1104, 1129
MvnI CGCG 1 cut(s) 465
NaeI GCCGGC 2 cut(s) 926, 1285
NciI CCSGG 7 cut(s) 447, 448, 921, 1008, 1035, 1197, 1323
NdeI CATATG 1 cut(s) 1147
NdeII GATC 2 cut(s) 363, 380
NgoMIV GCCGGC 2 cut(s) 924, 1283
NlaIII CATG 4 cut(s) 125, 146, 895, 1490
NlaIV GGNNCC 3 cut(s) 6, 96, 1320
NmeAIII GCCGAG 1 cut(s) 269
PdiI GCCGGC 2 cut(s) 926, 1285
PfeI GAWTC 2 cut(s) 604, 703
PleI GAGTC 4 cut(s) 279, 295, 312, 404
PmaCI CACGTG 1 cut(s) 458
PmlI CACGTG 1 cut(s) 458
PpsI GAGTC 4 cut(s) 279, 295, 312, 404
Ppu21I YACGTR 1 cut(s) 458
Psp1406I AACGTT 1 cut(s) 206
Psp6I CCWGG 3 cut(s) 367, 1102, 1127
PspCI CACGTG 1 cut(s) 458
PspGI CCWGG 3 cut(s) 367, 1102, 1127
PspN4I GGNNCC 3 cut(s) 6, 96, 1320
PspPI GGNCC 9 cut(s) 4, 371, 1036, 1193, 1227, 1239, 1335, 1359, 1371
PsuI RGATCY 2 cut(s) 363, 380
RigI GGCCGGCC 1 cut(s) 928
RsaI GTAC 2 cut(s) 251, 1507
RsaNI GTAC 2 cut(s) 250, 1506
RseI CAYNNNNRTG 1 cut(s) 896
SacII CCGCGG 1 cut(s) 466
SaqAI TTAA 2 cut(s) 1343, 1416
Sau3AI GATC 2 cut(s) 363, 380
Sau96I GGNCC 9 cut(s) 4, 371, 1036, 1193, 1227, 1239, 1335, 1359, 1371
SchI GAGTC 4 cut(s) 280, 295, 312, 404
SduI GDGCHC 3 cut(s) 879, 1013, 1128
SfaNI GCATC 3 cut(s) 568, 574, 580
SfcI CTRYAG 1 cut(s) 240
Sfr303I CCGCGG 1 cut(s) 466
SgrBI CCGCGG 1 cut(s) 466
SinI GGWCC 1 cut(s) 4
SmaI CCCGGG 1 cut(s) 448
SmiMI CAYNNNNRTG 1 cut(s) 896
SmlI CTYRAG 2 cut(s) 320, 338
SmoI CTYRAG 2 cut(s) 320, 338
SspMI CTAG 2 cut(s) 1014, 1494
StyI CCWWGG 1 cut(s) 1223
TaaI ACNGT 5 cut(s) 534, 678, 1060, 1135, 1510
TaiI ACGT 3 cut(s) 209, 460, 1111
TaqI TCGA 4 cut(s) 227, 255, 1022, 1064
TatI WGTACW 2 cut(s) 249, 1505
TauI GCSGC 8 cut(s) 46, 119, 465, 468, 523, 859, 1265, 1336
TfiI GAWTC 2 cut(s) 604, 703
Tru1I TTAA 2 cut(s) 1343, 1416
Tru9I TTAA 2 cut(s) 1343, 1416
TscAI CASTG 1 cut(s) 1294
TspDTI ATGAA 1 cut(s) 1503
TspMI CCCGGG 1 cut(s) 446
TspRI CASTG 1 cut(s) 1294
VpaK11BI GGWCC 1 cut(s) 4
XmaI CCCGGG 1 cut(s) 446
XmiI GTMKAC 2 cut(s) 264, 1306
XspI CTAG 2 cut(s) 1014, 1494
ZraI GACGTC 1 cut(s) 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.