Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002344

Physical Location & Seq

Genomic Coordinates Forward (+)

8887796 .. 8894136

Gene Structure 6341 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G008870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGTGTTGCTTCGAACACAGGGAAGAGTGTTGGGCTCTGTGTTGCTTCGTTGAGCTCTGTCTTCCAAGGTTGCCACGAACGACACCGCCGCCACCAACCCTGCAGCCGTACCGCCACCGTTATCGGATTTGGGGGCCTGAGGAAAGAGGTGGTGGGAGAAGCTCAAGGAGGGAACGAGGGACTAGAGGGGTCGCTATTTGGTTGATTGACATGTCGAAATGGAGAAAGGTGGATTCCAGGGTGTTTGGGATAACACGGTCCATGATTCCCGAGTCATCTTGGAACGTCTTGAGAATACTTCGAAGGGAAGGGTTTGAAGCCTACTTAGTGGGCGGATGTGTGAGGGACTTGATCCTGAAAAGAATTCCTAAAGACTTTGATGTGATCACTACAGCTAATCTTAAGCAGATCAAGAGGCAGTTCCATCGTGCACAAATCGTCGGACAACGATTTCCTATATGTATGGTGCATATTAAAGGTTCTGTCATAGAGGTTTCCAGTTTTGAAACGGTGGCAAAAGCAAAACACTCTGATAAAGAAGTTACTTTCTCTCAAATGCCAAAGGGCTGTGATAAGAAAGATTTCATCCGCTGGAGAAACAGCATGCATCGAGACTTCACAATTAACAGTTTGTTCTTCGATCCTTTATCAAATAAAATCTTTGATTATGCAAATGGAATGGCGGATTTGAGATCCTCAAAGCTACGGTCGTTAGGCTCCGCCAGGTTGTCGTTTAAAGAGGATTGCGCGAGAATTTTACGTGGCTTAAGAATTGCTGCTCGTCTGAGTTTGAAAATTTCAAAAGAAACTGAGACAGCTATGCATAAGTTTTCTTCATCCATTTCGACATTGGCAACGAGTAGAATTATGATGGAAATGAACTATATGCTTTCTTATGGAGCTGCTGAGCCATCTCTCTGTTTGCTTTGGGAATTCAATCTACTCAGAGTTCTACTTCCAATTCATGCAGCATACTTGGATGAACAGAGGATTAGGAAGTTTCCTCAGAATTCCACGATGTTGATGAAACTATTCTTTAATTTGGATAAAGTTGTCACGGTTGATCGACCTTCTGACTGCAGTTTATGGGTAGCTCTGTTGGCTTTTCACATGGCACTGGTCAGTCACCCTCAAGACGCTCTTGTGGTGTTCACCTTTGCTTCTGTCCTGTATCATGGGGGATGCAAAAAGGGTCTTACATTTGCTAGAGACAATGCTCAAGTGATAGTTGACTATCTGCCTGAGATCTCAAGTTCATCTGCATGTAAATCAGAGGAAGAGCTTGAAAAAGAAGTTTCCTTATTGGCCTCTCTTGTTTTAGATTCTATTGCTGCTCTAACTGCAACTGAGAGTCTTGTCGAAACGATGTCTAAGTATCCTGAATCTCCATGCTCTGGATTGGTATTTGTACCAAAGAAAACGGCCCAAGGTGTTGCTGAAATTTTTAGAGTTCTGGCCGACGACATCAAATCTTACAATAGAGAAAGAAAAAGTTATGAAATTGATTACCCTGTACTTGGGAAAGGTTTTCTGCACGAGACAAGATTTGTGCTTGGCAAAATTATTTTAGAAACCATGAGCAGTAGTATTTTGAAAGGCGAGGAATTTGTTCAGGAGGACTGCCGACACCTGGAGCAGGAGAGCATTACGGAAAATTGCAATAAGGGTAAAAAACGTGGCCTTAAACCTGATACTCCTAAACTAAAACAAGAGTCTGTGAAGAAACATAAGTTGGTCGAAAGGAAATGGAGACCGTTGGAGCAAGAGATGGACATTGATAACCAAGATGCTGTAATATTATCACAATTGGTTTCAGTTCCGAGACATAATGTAGAGAAGGAAGCATGCCAGTTACCTGAAGAGAAGGTGATAAAGAAAAAACATAAGGCAACGAAGCAGCAGTGTGTACCATGGGATGAGGCAACGAAGCAGCAACTAAAGACAGTTGAGAAGAATAATGACAAGGAGAATGACAGTCTATCTAGTCTCTTGAGCGGTGAGAAGATACAGAAACGCAAAGAAGTACCAGAAAAGGGGGAGAAAAGCAGTCAACTTCGACTGTCTAGTCTCTTCAAGGAAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004652 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006276 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0031123 GO:0031124 GO:0034641 GO:0043170 GO:0043412 GO:0043631 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0070566 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PolyA_pol PolyA_pol_RNAbd

Protein Analysis

694

Amino Acids

79.47

Weight (kDa)

9.21

Isoelectric Point (pI)

52.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PolyA_pol	PF01743	107 - 236	6.9e-25	Poly A polymerase head domain
PolyA_pol_RNAbd	PF12627	264 - 325	6.5e-15	Probable RNA and SrmB- binding site of polymerase A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17580
fragaria_vesca	FvH4_1g10510 FvH4_1g10510 FvH4_1g10510 FvH4_1g10510 FvH4_1g29700
malus_domestica	MD02G1119000.v1.1 MD15G1233000.v1.1
prunus_persica	Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1 Prupe.7G180900_v2.0.a1
pyrus_communis	pycom02g09260 pycom02g09270 pycom15g20750
rosa_chinensis	RchiOBHm_Chr2g0097411 RchiOBHm_Chr2g0097511 RchiOBHm_Chr3g0465401
rosa_laevigata	RLG00000016724
rosa_multiflora	Rmu_sc0001060.1_g000002 Rmu_sc0004220.1_g000008 Rmu_sc0011781.1_g000006
rosa_roxburghii	Rroxscaffold_2G00144710
rosa_rugosa	Rorug02G0066800 Rorug02G0066800 Rorug02G0066800
rosa_samantha	Rh2AG113200 Rh2BG116200 Rh2BG116300 Rh2CG118200 Rh2CG118300 Rh2DG117300 Rh3BG141600 Rh3CG142700 Rh3DG142700
rosa_wichuraiana	Rw2G008870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2064
AccB7I	CCANNNNNTGG	1 cut(s)	1394
AccBSI	CCGCTC	1 cut(s)	1995
AccII	CGCG	1 cut(s)	749
AciI	CCGC	8 cut(s)	86, 89, 112, 333, 589, 683, 720, 1995
AclWI	GGATC	3 cut(s)	346, 635, 687
AcoI	YGGCCR	1 cut(s)	1455
AcsI	RAATTY	7 cut(s)	363, 753, 795, 932, 1009, 1440, 1604
AcuI	CTGAAG	1 cut(s)	1878
AfaI	GTAC	5 cut(s)	110, 1410, 1515, 1908, 2025
AfiI	CCNNNNNNNGG	5 cut(s)	1394, 1517, 1630, 1636, 2033
AflII	CTTAAG	2 cut(s)	401, 766
AflIII	ACRYGT	1 cut(s)	210
AgsI	TTSAA	8 cut(s)	317, 506, 793, 801, 937, 1286, 1594, 2074
AjnI	CCWGG	3 cut(s)	236, 722, 1629
AjuI	GAANNNNNNNTTGG	2 cut(s)	1715, 1747
AluBI	AGCT	8 cut(s)	55, 162, 395, 703, 818, 902, 1094, 1282
AluI	AGCT	8 cut(s)	55, 162, 395, 703, 818, 902, 1094, 1282
Alw21I	GWGCWC	2 cut(s)	57, 433
Alw26I	GTCTC	8 cut(s)	606, 806, 1203, 1532, 1744, 1816, 1991, 2072
Alw44I	GTGCAC	1 cut(s)	429
AlwI	GGATC	3 cut(s)	346, 635, 687
Ama87I	CYCGRG	1 cut(s)	269
AoxI	GGCC	5 cut(s)	134, 1305, 1422, 1455, 1678
ApaLI	GTGCAC	1 cut(s)	429
ApeKI	GCWGC	7 cut(s)	103, 776, 902, 968, 1331, 1897, 1930
ApoI	RAATTY	7 cut(s)	363, 753, 795, 932, 1009, 1440, 1604
ArsI	GACNNNNNNTTYG	2 cut(s)	692, 724
Asp700I	GAANNNNTTC	3 cut(s)	581, 1031, 1608
AspLEI	GCGC	1 cut(s)	749
AspS9I	GGNCC	3 cut(s)	134, 258, 1423
AsuHPI	GGTGA	4 cut(s)	1118, 1144, 1879, 2009
AsuII	TTCGAA	2 cut(s)	12, 301
AvaI	CYCGRG	1 cut(s)	269
AvaII	GGWCC	1 cut(s)	258
AxyI	CCTNAGG	1 cut(s)	138
BaeGI	GKGCMC	1 cut(s)	433
BanII	GRGCYC	2 cut(s)	37, 57
BauI	CACGAG	1 cut(s)	1535
BbsI	GAAGAC	1 cut(s)	53
Bbv12I	GWGCWC	2 cut(s)	57, 433
BbvI	GCAGC	7 cut(s)	115, 763, 889, 980, 1318, 1909, 1942
BccI	CCATC	4 cut(s)	432, 865, 919, 1762
BceAI	ACGGC	2 cut(s)	91, 1437
BcgI	CGANNNNNNTGC	2 cut(s)	407, 441
BciT130I	CCWGG	3 cut(s)	238, 724, 1631
BciVI	GTATCC	1 cut(s)	1386
BclI	TGATCA	1 cut(s)	384
BcoDI	GTCTC	8 cut(s)	606, 806, 1203, 1532, 1744, 1816, 1991, 2072
BfaI	CTAG	4 cut(s)	183, 1206, 1983, 2064
BfmI	CTRYAG	3 cut(s)	101, 390, 1078
BfrI	CTTAAG	2 cut(s)	401, 766
BfuI	GTATCC	1 cut(s)	1386
BglII	AGATCT	1 cut(s)	1245
BisI	GCNGC	8 cut(s)	89, 104, 777, 903, 969, 1332, 1898, 1931
BlpI	GCTNAGC	1 cut(s)	906
BlsI	GCNGC	8 cut(s)	90, 105, 778, 904, 970, 1333, 1899, 1932
Bme1390I	CCNGG	3 cut(s)	238, 724, 1631
Bme18I	GGWCC	1 cut(s)	258
BmeT110I	CYCGRG	1 cut(s)	269
BmgT120I	GGNCC	3 cut(s)	134, 258, 1423
BmiI	GGNNCC	2 cut(s)	135, 718
BmrFI	CCNGG	3 cut(s)	238, 724, 1631
BmsI	GCATC	3 cut(s)	616, 1172, 1777
BpiI	GAAGAC	1 cut(s)	53
BpmI	CTGGAG	2 cut(s)	613, 1652
Bpu1102I	GCTNAGC	1 cut(s)	906
Bpu14I	TTCGAA	2 cut(s)	12, 301
BpuEI	CTTGAG	6 cut(s)	148, 310, 1116, 1203, 1234, 2011
BsaAI	YACGTR	1 cut(s)	761
BsaI	GGTCTC	1 cut(s)	1744
BsaJI	CCNNGG	4 cut(s)	65, 237, 1426, 1910
Bsc4I	CCNNNNNNNGG	5 cut(s)	1394, 1517, 1630, 1636, 2033
Bse1I	ACTGG	3 cut(s)	498, 1122, 1849
Bse21I	CCTNAGG	1 cut(s)	138
BseBI	CCWGG	3 cut(s)	238, 724, 1631
BseDI	CCNNGG	4 cut(s)	65, 237, 1426, 1910
BseGI	GGATG	6 cut(s)	341, 585, 836, 985, 1187, 1921
BseLI	CCNNNNNNNGG	5 cut(s)	1394, 1517, 1630, 1636, 2033
BseMII	CTCAG	8 cut(s)	129, 776, 801, 897, 958, 1019, 1233, 1338
BseNI	ACTGG	3 cut(s)	498, 1122, 1849
BseSI	GKGCMC	1 cut(s)	433
BseXI	GCAGC	7 cut(s)	115, 763, 889, 980, 1318, 1909, 1942
BsgI	GTGCAG	1 cut(s)	1517
Bsh1236I	CGCG	1 cut(s)	749
Bsh1285I	CGRYCG	1 cut(s)	710
BshFI	GGCC	5 cut(s)	136, 1307, 1424, 1457, 1680
BsiEI	CGRYCG	1 cut(s)	710
BsiHKAI	GWGCWC	2 cut(s)	57, 433
BsiHKCI	CYCGRG	1 cut(s)	269
BslFI	GGGAC	2 cut(s)	193, 359
BslI	CCNNNNNNNGG	5 cut(s)	1394, 1517, 1630, 1636, 2033
BsmAI	GTCTC	8 cut(s)	606, 806, 1203, 1532, 1744, 1816, 1991, 2072
BsmFI	GGGAC	2 cut(s)	193, 359
BsnI	GGCC	5 cut(s)	136, 1307, 1424, 1457, 1680
Bso31I	GGTCTC	1 cut(s)	1744
BsoBI	CYCGRG	1 cut(s)	269
Bsp119I	TTCGAA	2 cut(s)	12, 301
Bsp1286I	GDGCHC	3 cut(s)	37, 57, 433
Bsp143I	GATC	7 cut(s)	351, 384, 408, 640, 692, 1063, 1245
Bsp1720I	GCTNAGC	1 cut(s)	906
Bsp19I	CCATGG	1 cut(s)	1910
BspACI	CCGC	8 cut(s)	86, 89, 112, 333, 589, 683, 720, 1995
BspANI	GGCC	5 cut(s)	136, 1307, 1424, 1457, 1680
BspCNI	CTCAG	8 cut(s)	130, 777, 802, 898, 957, 1018, 1234, 1339
BspFNI	CGCG	1 cut(s)	749
BspLI	GGNNCC	2 cut(s)	135, 718
BspMAI	CTGCAG	2 cut(s)	105, 1082
BspPI	GGATC	3 cut(s)	346, 635, 687
BspQI	GCTCTTC	1 cut(s)	1272
BspT104I	TTCGAA	2 cut(s)	12, 301
BspTI	CTTAAG	2 cut(s)	401, 766
BspTNI	GGTCTC	1 cut(s)	1744
BsrBI	CCGCTC	1 cut(s)	1995
BsrI	ACTGG	3 cut(s)	498, 1122, 1849
BssECI	CCNNGG	4 cut(s)	65, 237, 1426, 1910
BssMI	GATC	7 cut(s)	351, 384, 408, 640, 692, 1063, 1245
BssSI	CACGAG	1 cut(s)	1535
BssT1I	CCWWGG	3 cut(s)	65, 1426, 1910
Bst2BI	CACGAG	1 cut(s)	1535
Bst2UI	CCWGG	3 cut(s)	238, 724, 1631
Bst6I	CTCTTC	4 cut(s)	18, 1272, 1854, 2075
BstAFI	CTTAAG	2 cut(s)	401, 766
BstBAI	YACGTR	1 cut(s)	761
BstBI	TTCGAA	2 cut(s)	12, 301
BstC8I	GCNNGC	2 cut(s)	605, 1846
BstDSI	CCRYGG	1 cut(s)	1910
BstENI	CCTNNNNNAGG	1 cut(s)	1634
BstF5I	GGATG	6 cut(s)	341, 585, 836, 985, 1187, 1921
BstFNI	CGCG	1 cut(s)	749
BstHHI	GCGC	1 cut(s)	749
BstKTI	GATC	7 cut(s)	354, 387, 411, 643, 695, 1066, 1248
BstMAI	GTCTC	8 cut(s)	606, 806, 1203, 1532, 1744, 1816, 1991, 2072
BstMBI	GATC	7 cut(s)	351, 384, 408, 640, 692, 1063, 1245
BstMCI	CGRYCG	1 cut(s)	710
BstMWI	GCNNNNNNNGC	2 cut(s)	1100, 1340
BstNI	CCWGG	3 cut(s)	238, 724, 1631
BstNSI	RCATGY	4 cut(s)	214, 607, 1266, 1848
BstSCI	CCNGG	3 cut(s)	236, 722, 1629
BstSFI	CTRYAG	3 cut(s)	101, 390, 1078
BstSLI	GKGCMC	1 cut(s)	433
BstUI	CGCG	1 cut(s)	749
BstV1I	GCAGC	7 cut(s)	115, 763, 889, 980, 1318, 1909, 1942
BstV2I	GAAGAC	1 cut(s)	53
BstX2I	RGATCY	2 cut(s)	692, 1245
BstYI	RGATCY	2 cut(s)	692, 1245
Bsu36I	CCTNAGG	1 cut(s)	138
BsuI	GTATCC	1 cut(s)	1386
BsuRI	GGCC	5 cut(s)	136, 1307, 1424, 1457, 1680
BtgI	CCRYGG	1 cut(s)	1910
BtsCI	GGATG	6 cut(s)	341, 585, 836, 985, 1187, 1921
BtsI	GCAGTG	1 cut(s)	1907
BtsIMutI	CAGTG	2 cut(s)	1115, 1907
Cac8I	GCNNGC	2 cut(s)	605, 1846
CfoI	GCGC	1 cut(s)	749
Cfr13I	GGNCC	3 cut(s)	134, 258, 1423
CseI	GACGC	1 cut(s)	1145
Csp6I	GTAC	5 cut(s)	109, 1409, 1514, 1907, 2024
CviQI	GTAC	5 cut(s)	109, 1409, 1514, 1907, 2024
DpnI	GATC	7 cut(s)	353, 386, 410, 642, 694, 1065, 1247
DpnII	GATC	7 cut(s)	351, 384, 408, 640, 692, 1063, 1245
DraI	TTTAAA	1 cut(s)	736
DrdI	GACNNNNNNGTC	1 cut(s)	2064
DseDI	GACNNNNNNGTC	1 cut(s)	2064
EaeI	YGGCCR	1 cut(s)	1455
Eam1104I	CTCTTC	4 cut(s)	18, 1272, 1854, 2075
EarI	CTCTTC	4 cut(s)	18, 1272, 1854, 2075
EciI	GGCGGA	3 cut(s)	348, 698, 709
Ecl136II	GAGCTC	1 cut(s)	55
Eco130I	CCWWGG	3 cut(s)	65, 1426, 1910
Eco24I	GRGCYC	2 cut(s)	37, 57
Eco31I	GGTCTC	1 cut(s)	1744
Eco47I	GGWCC	1 cut(s)	258
Eco53kI	GAGCTC	1 cut(s)	55
Eco57I	CTGAAG	1 cut(s)	1878
Eco81I	CCTNAGG	1 cut(s)	138
Eco88I	CYCGRG	1 cut(s)	269
EcoICRI	GAGCTC	1 cut(s)	55
EcoNI	CCTNNNNNAGG	1 cut(s)	1634
EcoO109I	RGGNCCY	1 cut(s)	134
EcoRI	GAATTC	3 cut(s)	363, 932, 1009
EcoRII	CCWGG	3 cut(s)	236, 722, 1629
EcoT14I	CCWWGG	3 cut(s)	65, 1426, 1910
EcoT22I	ATGCAT	2 cut(s)	609, 825
EcoT38I	GRGCYC	2 cut(s)	37, 57
ErhI	CCWWGG	3 cut(s)	65, 1426, 1910
FalI	AAGNNNNNCTT	2 cut(s)	1125, 1157
FaqI	GGGAC	2 cut(s)	193, 359
FbaI	TGATCA	1 cut(s)	384
Fnu4HI	GCNGC	8 cut(s)	89, 104, 777, 903, 969, 1332, 1898, 1931
FokI	GGATG	6 cut(s)	348, 572, 823, 992, 1194, 1928
FriOI	GRGCYC	2 cut(s)	37, 57
Fsp4HI	GCNGC	8 cut(s)	89, 104, 777, 903, 969, 1332, 1898, 1931
FspBI	CTAG	4 cut(s)	183, 1206, 1983, 2064
GlaI	GCGC	1 cut(s)	748
GluI	GCNGC	8 cut(s)	89, 104, 777, 903, 969, 1332, 1898, 1931
GsuI	CTGGAG	2 cut(s)	613, 1652
HaeIII	GGCC	5 cut(s)	136, 1307, 1424, 1457, 1680
HgaI	GACGC	1 cut(s)	1145
HhaI	GCGC	1 cut(s)	749
Hin6I	GCGC	1 cut(s)	747
HinP1I	GCGC	1 cut(s)	747
HincII	GTYRAC	2 cut(s)	1231, 2051
HindII	GTYRAC	2 cut(s)	1231, 2051
HinfI	GANTC	7 cut(s)	233, 265, 272, 1322, 1351, 1382, 1712
HphI	GGTGA	4 cut(s)	1118, 1144, 1879, 2009
Hpy166II	GTNNAC	5 cut(s)	431, 1152, 1231, 1907, 2051
Hpy188I	TCNGA	9 cut(s)	126, 443, 532, 786, 947, 1008, 1075, 1273, 1821
Hpy8I	GTNNAC	5 cut(s)	431, 1152, 1231, 1907, 2051
Hpy99I	CGWCG	2 cut(s)	443, 1463
HpyAV	CCTTC	5 cut(s)	297, 302, 1080, 1831, 1858
HpyCH4IV	ACGT	3 cut(s)	285, 760, 1675
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1100, 1340
HpySE526I	ACGT	3 cut(s)	285, 760, 1675
HspAI	GCGC	1 cut(s)	747
Ksp22I	TGATCA	1 cut(s)	384
Kzo9I	GATC	7 cut(s)	351, 384, 408, 640, 692, 1063, 1245
LguI	GCTCTTC	1 cut(s)	1272
LmnI	GCTCC	4 cut(s)	722, 899, 1633, 1759
Lsp1109I	GCAGC	7 cut(s)	115, 763, 889, 980, 1318, 1909, 1942
LweI	GCATC	3 cut(s)	616, 1172, 1777
MaeI	CTAG	4 cut(s)	183, 1206, 1983, 2064
MaeII	ACGT	3 cut(s)	285, 760, 1675
MaeIII	GTNAC	4 cut(s)	541, 1054, 1124, 1851
MalI	GATC	7 cut(s)	353, 386, 410, 642, 694, 1065, 1247
MbiI	CCGCTC	1 cut(s)	1995
MboI	GATC	7 cut(s)	351, 384, 408, 640, 692, 1063, 1245
MfeI	CAATTG	1 cut(s)	1805
MflI	RGATCY	2 cut(s)	692, 1245
MhlI	GDGCHC	3 cut(s)	37, 57, 433
MlyI	GAGTC	3 cut(s)	281, 1360, 1721
MmeI	TCCRAC	2 cut(s)	421, 1737
Mph1103I	ATGCAT	2 cut(s)	609, 825
MroXI	GAANNNNTTC	3 cut(s)	581, 1031, 1608
MseI	TTAA	7 cut(s)	402, 474, 624, 735, 767, 1038, 1683
MslI	CAYNNNNRTG	1 cut(s)	1261
MspA1I	CMGCKG	1 cut(s)	591
MspCI	CTTAAG	2 cut(s)	401, 766
MspR9I	CCNGG	3 cut(s)	238, 724, 1631
MunI	CAATTG	1 cut(s)	1805
MvaI	CCWGG	3 cut(s)	238, 724, 1631
MvnI	CGCG	1 cut(s)	749
MwoI	GCNNNNNNNGC	2 cut(s)	1100, 1340
NcoI	CCATGG	1 cut(s)	1910
NdeII	GATC	7 cut(s)	351, 384, 408, 640, 692, 1063, 1245
NlaIV	GGNNCC	2 cut(s)	135, 718
NmuCI	GTSAC	2 cut(s)	1054, 1124
NsiI	ATGCAT	2 cut(s)	609, 825
NspI	RCATGY	4 cut(s)	214, 607, 1266, 1848
NspV	TTCGAA	2 cut(s)	12, 301
PaeI	GCATGC	2 cut(s)	607, 1848
PciI	ACATGT	1 cut(s)	210
PciSI	GCTCTTC	1 cut(s)	1272
PdmI	GAANNNNTTC	3 cut(s)	581, 1031, 1608
PfeI	GAWTC	4 cut(s)	233, 265, 1322, 1382
PflMI	CCANNNNNTGG	1 cut(s)	1394
PkrI	GCNGC	8 cut(s)	90, 105, 778, 904, 970, 1333, 1899, 1932
PleI	GAGTC	3 cut(s)	280, 1359, 1720
PpsI	GAGTC	3 cut(s)	280, 1359, 1720
Ppu21I	YACGTR	1 cut(s)	761
PscI	ACATGT	1 cut(s)	210
Psp124BI	GAGCTC	1 cut(s)	57
Psp6I	CCWGG	3 cut(s)	236, 722, 1629
PspGI	CCWGG	3 cut(s)	236, 722, 1629
PspN4I	GGNNCC	2 cut(s)	135, 718
PspPI	GGNCC	3 cut(s)	134, 258, 1423
PstI	CTGCAG	2 cut(s)	105, 1082
PsuI	RGATCY	2 cut(s)	692, 1245
RsaI	GTAC	5 cut(s)	110, 1410, 1515, 1908, 2025
RsaNI	GTAC	5 cut(s)	109, 1409, 1514, 1907, 2024
RseI	CAYNNNNRTG	1 cut(s)	1261
SacI	GAGCTC	1 cut(s)	57
SapI	GCTCTTC	1 cut(s)	1272
SaqAI	TTAA	7 cut(s)	402, 474, 624, 735, 767, 1038, 1683
SatI	GCNGC	8 cut(s)	89, 104, 777, 903, 969, 1332, 1898, 1931
Sau3AI	GATC	7 cut(s)	351, 384, 408, 640, 692, 1063, 1245
Sau96I	GGNCC	3 cut(s)	134, 258, 1423
SchI	GAGTC	3 cut(s)	281, 1360, 1721
ScrFI	CCNGG	3 cut(s)	238, 724, 1631
SduI	GDGCHC	3 cut(s)	37, 57, 433
SfaNI	GCATC	3 cut(s)	616, 1172, 1777
SfcI	CTRYAG	3 cut(s)	101, 390, 1078
SfuI	TTCGAA	2 cut(s)	12, 301
SinI	GGWCC	1 cut(s)	258
SmiMI	CAYNNNNRTG	1 cut(s)	1261
SmlI	CTYRAG	8 cut(s)	163, 289, 401, 766, 1131, 1218, 1249, 1990
SmoI	CTYRAG	8 cut(s)	163, 289, 401, 766, 1131, 1218, 1249, 1990
SphI	GCATGC	2 cut(s)	607, 1848
SsiI	CCGC	8 cut(s)	86, 89, 112, 333, 589, 683, 720, 1995
SspI	AATATT	1 cut(s)	1797
SspMI	CTAG	4 cut(s)	183, 1206, 1983, 2064
SstI	GAGCTC	1 cut(s)	57
StyD4I	CCNGG	3 cut(s)	236, 722, 1629
StyI	CCWWGG	3 cut(s)	65, 1426, 1910
TaiI	ACGT	3 cut(s)	288, 763, 1678
TatI	WGTACW	1 cut(s)	1513
TauI	GCSGC	1 cut(s)	91
TfiI	GAWTC	4 cut(s)	233, 265, 1322, 1382
Tru1I	TTAA	7 cut(s)	402, 474, 624, 735, 767, 1038, 1683
Tru9I	TTAA	7 cut(s)	402, 474, 624, 735, 767, 1038, 1683
TscAI	CASTG	2 cut(s)	1122, 1907
TseFI	GTSAC	2 cut(s)	1054, 1124
TseI	GCWGC	7 cut(s)	103, 776, 902, 968, 1331, 1897, 1930
Tsp45I	GTSAC	2 cut(s)	1054, 1124
TspDTI	ATGAA	8 cut(s)	574, 825, 893, 953, 996, 1040, 1245, 1512
TspGWI	ACGGA	1 cut(s)	1664
TspRI	CASTG	2 cut(s)	1122, 1907
Van91I	CCANNNNNTGG	1 cut(s)	1394
Vha464I	CTTAAG	2 cut(s)	401, 766
VneI	GTGCAC	1 cut(s)	429
VpaK11BI	GGWCC	1 cut(s)	258
XagI	CCTNNNNNAGG	1 cut(s)	1634
XapI	RAATTY	7 cut(s)	363, 753, 795, 932, 1009, 1440, 1604
XceI	RCATGY	4 cut(s)	214, 607, 1266, 1848
XcmI	CCANNNNNNNNNTGG	1 cut(s)	847
XmnI	GAANNNNTTC	3 cut(s)	581, 1031, 1608
XspI	CTAG	4 cut(s)	183, 1206, 1983, 2064
Zsp2I	ATGCAT	2 cut(s)	609, 825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.