Rw4G020640

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013202
Physical Location & Seq
Forward (+)
44668968 .. 44671114
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020640.1

Sequence Viewer

Length: 1671 bp
ATGGCGGGTGCGCGTCTTTGCGCCACCTTCCCCAATCTCGCACACCCAATCTTACCCGCCACCACCCCGCCCAAAATTCCTGTATCAGCCTCCGTTTCCGTACCGTCAAAAATCACTCGCCGGAAGAACTATTTGCGCCCCAAAATTCTCAAAACCCTAAACTCTAAACCCGACCCGCAACCCGTAGTTCCTGTCGAATCTCCAGTAACCCCATCCCAAGATAACCATGAGCTCGAGCATAGCTCCGACCGAGGCGACGTCGCTTTGGGAGGAGGAGCTGAAGTCGAAGAATTAAGAGTGTCCGAGACGACGGCGGAGCTCAGTGGCATTTCTGGTAAATTTTCTGCGAGGACTGTGCTAAAGTATGGGGGTTATTTTATTGGGCTTTTTGTGCTACAGACTGTTGTTTCTGTTTTGTTATTGGGAGATAGTGATCCTGATGAAAATAGCAAGTCTAGGAGTTCAGTATTAGGCAAATCCAATTTGAAGACGAGTTCTGGGTCTGAATTAGGCAATGTGGTTCGGTTTGATGAGTCCCAATTGGGAGAGAAAATTGAAGAGATTAGGGCAATGGCAAGGAAAGCGCGAAAGATTGAGAAGAATGAGTCCAAGGGAAGTATTGGAAATGAGGATGATGCATTTTCGAAGTCTAAGAACAAGATTGGGATTGAAAAGGAGGTTGGGAACCGGTTGGTTAACTTGCAGAAGAGGTTGGGTTCCAAAAGGGAGAAGTTGCCGGGTTCGGGGGTGAGGAAGAATAATGAGAATGTGAAGGAGGGGGATGGGGCGTTGATGTTTAAGAAGAAGCTGAAGTTTAAGACTCCTACTGCTGAGGTGAATAGGAATCCGAAGGGTTTTGGAGGTGTCAAAGAGGAAGGTGGTTCTAGTAAGAGTAAGAGAGGTTCAGGTGGTGTAGATGAAATTGGGAGAAATGCAAGTGTTGGTACTGATGGTATGGAATTGCTGGAAGCTGATGCTAAGGAGAAGCCGCGAGAGACTTTGGAGAAAGGAACAGGGAGATTAGATGAAGGACAGGACAGAGAAGCTGGAAATATGGTACCGCAGAATGGTACTGTCCACAGGACTATACAGGGGAGATCATCTGCTGAAGATGTAAAGTCAATCAAACCAAGAGGCTTCGGGAAGAAAAACCCTGAAAGCTTGAAGAAAGAAATTCAGGGAAGGACAGTAGAATCAGATGGTCCTACCGTGCAGAGTGTGAATAGCAGTTCAACACAGAAAAAAGCCAAAAAACAAGCACTGGCTAACAAAGTCAGTAGTAAGCAGTCAGATAATGAGACTGATCCATGGTGGTTGCATCTTCCTTATGTTCTAGTTATTTTTATGCGTAGAGGTTCTGATGGGCAAGGAGGGCTTTTTAGCATAGAGCTCAATTCTCAGTCCCAAAATGAGAGTGATGCTTCCTATACTGTTGCTTTTGAGGACCGTGGTGATGCCAATAACTTCTGTTTCCTATTGGAGTCTTGTTTTGAAGATCAAGATGATTTCAGGGCCACTGCTGCTCCCTTGCCAAATAAAGAACTTCATAAAGCAGTAAAATCAGATGACATGAAGGTAATTGTTGTGAAGAAGGGGCAGCTTCAGCTGTATGCTGGGCAACCTTTTGCTGAAGTTGAAATGGCTTTGCGCTCTTTAGTTTTACAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3110

Protein Analysis

556

Amino Acids

60.41

Weight (kDa)

9.19

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3110 PF11360 455 - 534 4.6e-06 Protein of unknown function (DUF3110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15820
fragaria_vesca FvH4_4g18220
malus_domestica MD13G1168100.v1.1
prunus_persica Prupe.1G135700_v2.0.a1 Prupe.1G135700_v2.0.a1 Prupe.1G135700_v2.0.a1
pyrus_communis pycom13g14390
rosa_chinensis RchiOBHm_Chr4g0422351
rosa_laevigata RLG00000007617
rosa_multiflora Rmu_sc0013245.1_g000011
rosa_roxburghii Rroxscaffold_5G00364460 Rroxscaffold_5G00364470
rosa_rugosa Rorug04G0179300
rosa_samantha Rh4AG238700 Rh4BG242300 Rh4CG253800 Rh4DG237700
rosa_wichuraiana Rw4G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
Acc65I GGTACC 1 cut(s) 1057
AccB1I GGYRCC 1 cut(s) 1057
AccII CGCG 3 cut(s) 13, 586, 991
AciI CCGC 7 cut(s) 5, 57, 68, 176, 314, 989, 1061
AclWI GGATC 2 cut(s) 428, 1298
AcsI RAATTY 4 cut(s) 75, 144, 338, 1173
AcuI CTGAAG 5 cut(s) 300, 830, 1128, 1586, 1650
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 4 cut(s) 102, 946, 1059, 1072
AfiI CCNNNNNNNGG 2 cut(s) 743, 1067
AgeI ACCGGT 1 cut(s) 687
AgsI TTSAA 7 cut(s) 487, 557, 671, 1165, 1233, 1493, 1637
AjuI GAANNNNNNNTTGG 2 cut(s) 663, 695
Alw21I GWGCWC 3 cut(s) 234, 321, 1392
Alw26I GTCTC 3 cut(s) 299, 989, 1292
AlwI GGATC 2 cut(s) 428, 1298
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 1512
ApeKI GCWGC 2 cut(s) 1520, 1597
ApoI RAATTY 4 cut(s) 75, 144, 338, 1173
AsiGI ACCGGT 1 cut(s) 687
Asp718I GGTACC 1 cut(s) 1057
AspLEI GCGC 5 cut(s) 13, 23, 138, 586, 1650
AspS9I GGNCC 3 cut(s) 1202, 1444, 1512
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 3 cut(s) 760, 847, 1463
AsuII TTCGAA 1 cut(s) 644
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 2 cut(s) 1202, 1444
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 3 cut(s) 234, 321, 1392
BbsI GAAGAC 1 cut(s) 494
Bbv12I GWGCWC 3 cut(s) 234, 321, 1392
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 2 cut(s) 1507, 1609
BccI CCATC 5 cut(s) 220, 776, 944, 1193, 1355
BceAI ACGGC 1 cut(s) 327
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 3 cut(s) 299, 989, 1292
BfaI CTAG 3 cut(s) 456, 885, 1334
BfmI CTRYAG 1 cut(s) 395
BisI GCNGC 3 cut(s) 989, 1521, 1598
BlsI GCNGC 3 cut(s) 990, 1522, 1599
Bme1390I CCNGG 1 cut(s) 738
Bme18I GGWCC 2 cut(s) 1202, 1444
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 3 cut(s) 1202, 1444, 1512
BmiI GGNNCC 3 cut(s) 686, 718, 1059
BmrFI CCNGG 1 cut(s) 738
BmsI GCATC 5 cut(s) 625, 964, 1327, 1408, 1444
BpiI GAAGAC 1 cut(s) 494
BplI GAGNNNNNCTC 2 cut(s) 227, 259
BpmI CTGGAG 1 cut(s) 186
Bpu10I CCTNAGC 2 cut(s) 831, 978
Bpu14I TTCGAA 1 cut(s) 644
BpuMI CCSGG 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 4 cut(s) 250, 609, 1307, 1447
BsaWI WCCGGW 1 cut(s) 687
BsaXI ACNNNNNCTCC 4 cut(s) 267, 297, 1507, 1537
Bsc4I CCNNNNNNNGG 2 cut(s) 743, 1067
Bse118I RCCGGY 1 cut(s) 687
Bse1I ACTGG 2 cut(s) 203, 1266
Bse3DI GCAATG 2 cut(s) 520, 576
Bse8I GATNNNNATC 1 cut(s) 432
BseDI CCNNGG 4 cut(s) 250, 609, 1307, 1447
BseGI GGATG 3 cut(s) 212, 637, 787
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 743, 1067
BseMI GCAATG 2 cut(s) 520, 576
BseMII CTCAG 3 cut(s) 334, 822, 1412
BseNI ACTGG 2 cut(s) 203, 1266
BseRI GAGGAG 2 cut(s) 285, 288
BseXI GCAGC 2 cut(s) 1507, 1609
BseYI CCCAGC 1 cut(s) 1613
BsgI GTGCAG 1 cut(s) 1232
Bsh1236I CGCG 3 cut(s) 13, 586, 991
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 1 cut(s) 1514
BshNI GGYRCC 1 cut(s) 1057
BshTI ACCGGT 1 cut(s) 687
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 3 cut(s) 234, 321, 1392
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 3 cut(s) 121, 688, 737
BslFI GGGAC 2 cut(s) 520, 1387
BslI CCNNNNNNNGG 2 cut(s) 743, 1067
BsmAI GTCTC 3 cut(s) 299, 989, 1292
BsmBI CGTCTC 1 cut(s) 299
BsmFI GGGAC 2 cut(s) 520, 1387
BsnI GGCC 1 cut(s) 1514
BsoBI CYCGRG 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 644
Bsp1286I GDGCHC 3 cut(s) 234, 321, 1392
Bsp143I GATC 4 cut(s) 433, 1097, 1303, 1495
Bsp19I CCATGG 1 cut(s) 1307
BspACI CCGC 7 cut(s) 5, 57, 68, 176, 314, 989, 1061
BspANI GGCC 1 cut(s) 1514
BspCNI CTCAG 3 cut(s) 333, 823, 1411
BspFNI CGCG 3 cut(s) 13, 586, 991
BspLI GGNNCC 3 cut(s) 686, 718, 1059
BspPI GGATC 2 cut(s) 428, 1298
BspT104I TTCGAA 1 cut(s) 644
BspT107I GGYRCC 1 cut(s) 1057
BsrDI GCAATG 2 cut(s) 520, 576
BsrFI RCCGGY 1 cut(s) 687
BsrI ACTGG 2 cut(s) 203, 1266
BssAI RCCGGY 1 cut(s) 687
BssECI CCNNGG 4 cut(s) 250, 609, 1307, 1447
BssMI GATC 4 cut(s) 433, 1097, 1303, 1495
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 2 cut(s) 609, 1307
Bst4CI ACNGT 8 cut(s) 105, 355, 403, 1075, 1189, 1210, 1432, 1448
Bst6I CTCTTC 2 cut(s) 552, 701
BstACI GRCGYC 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 644
BstDEI CTNAG 5 cut(s) 320, 651, 831, 978, 1398
BstDSI CCRYGG 2 cut(s) 1307, 1447
BstF5I GGATG 3 cut(s) 212, 637, 787
BstFNI CGCG 3 cut(s) 13, 586, 991
BstHHI GCGC 5 cut(s) 13, 23, 138, 586, 1650
BstKTI GATC 4 cut(s) 436, 1100, 1306, 1498
BstMAI GTCTC 3 cut(s) 299, 989, 1292
BstMBI GATC 4 cut(s) 433, 1097, 1303, 1495
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 5 cut(s) 391, 581, 1372, 1520, 1603
BstSCI CCNGG 1 cut(s) 736
BstSFI CTRYAG 1 cut(s) 395
BstUI CGCG 3 cut(s) 13, 586, 991
BstV1I GCAGC 2 cut(s) 1507, 1609
BstV2I GAAGAC 1 cut(s) 494
BsuRI GGCC 1 cut(s) 1514
BtgI CCRYGG 2 cut(s) 1307, 1447
BtsCI GGATG 3 cut(s) 212, 637, 787
BtsI GCAGTG 1 cut(s) 1515
BtsIMutI CAGTG 3 cut(s) 328, 1259, 1515
CfoI GCGC 5 cut(s) 13, 23, 138, 586, 1650
Cfr10I RCCGGY 1 cut(s) 687
Cfr13I GGNCC 3 cut(s) 1202, 1444, 1512
CseI GACGC 1 cut(s) 2
Csp6I GTAC 4 cut(s) 101, 945, 1058, 1071
CspAI ACCGGT 1 cut(s) 687
CviAII CATG 3 cut(s) 227, 1308, 1570
CviQI GTAC 4 cut(s) 101, 945, 1058, 1071
DdeI CTNAG 5 cut(s) 320, 651, 831, 978, 1398
DpnI GATC 4 cut(s) 435, 1099, 1305, 1497
DpnII GATC 4 cut(s) 433, 1097, 1303, 1495
Eam1104I CTCTTC 2 cut(s) 552, 701
EarI CTCTTC 2 cut(s) 552, 701
EciI GGCGGA 1 cut(s) 329
Ecl136II GAGCTC 3 cut(s) 232, 319, 1390
Eco130I CCWWGG 2 cut(s) 609, 1307
Eco24I GRGCYC 3 cut(s) 234, 321, 1392
Eco47I GGWCC 2 cut(s) 1202, 1444
Eco53kI GAGCTC 3 cut(s) 232, 319, 1390
Eco57I CTGAAG 5 cut(s) 300, 830, 1128, 1586, 1650
Eco88I CYCGRG 1 cut(s) 233
EcoICRI GAGCTC 3 cut(s) 232, 319, 1390
EcoT14I CCWWGG 2 cut(s) 609, 1307
EcoT22I ATGCAT 1 cut(s) 640
EcoT38I GRGCYC 3 cut(s) 234, 321, 1392
ErhI CCWWGG 2 cut(s) 609, 1307
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 3 cut(s) 230, 1311, 1573
FalI AAGNNNNNCTT 2 cut(s) 1359, 1391
FaqI GGGAC 2 cut(s) 520, 1387
FatI CATG 3 cut(s) 226, 1307, 1569
FauI CCCGC 3 cut(s) 64, 75, 183
Fnu4HI GCNGC 3 cut(s) 989, 1521, 1598
FokI GGATG 3 cut(s) 199, 644, 794
FriOI GRGCYC 3 cut(s) 234, 321, 1392
Fsp4HI GCNGC 3 cut(s) 989, 1521, 1598
FspBI CTAG 3 cut(s) 456, 885, 1334
GlaI GCGC 5 cut(s) 12, 22, 137, 585, 1649
GluI GCNGC 3 cut(s) 989, 1521, 1598
GsaI CCCAGC 1 cut(s) 1617
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 1 cut(s) 1514
HapII CCGG 3 cut(s) 121, 688, 737
HgaI GACGC 1 cut(s) 2
HhaI GCGC 5 cut(s) 13, 23, 138, 586, 1650
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 3 cut(s) 230, 1311, 1573
Hin6I GCGC 5 cut(s) 11, 21, 136, 584, 1648
HinP1I GCGC 5 cut(s) 11, 21, 136, 584, 1648
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 1 cut(s) 1159
HinfI GANTC 7 cut(s) 197, 533, 605, 820, 844, 1193, 1481
HpaI GTTAAC 1 cut(s) 697
HpaII CCGG 3 cut(s) 121, 688, 737
HphI GGTGA 3 cut(s) 760, 847, 1463
Hpy166II GTNNAC 2 cut(s) 697, 1078
Hpy188I TCNGA 8 cut(s) 247, 304, 505, 849, 1198, 1291, 1360, 1564
Hpy188III TCNNGA 3 cut(s) 437, 1141, 1499
Hpy8I GTNNAC 2 cut(s) 697, 1078
Hpy99I CGWCG 3 cut(s) 260, 263, 313
HpyAV CCTTC 8 cut(s) 37, 766, 844, 869, 1022, 1176, 1567, 1585
HpyCH4III ACNGT 8 cut(s) 105, 355, 403, 1075, 1189, 1210, 1432, 1448
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 5 cut(s) 638, 703, 935, 1213, 1318
HpyF10VI GCNNNNNNNGC 5 cut(s) 391, 581, 1372, 1520, 1603
HpyF3I CTNAG 5 cut(s) 320, 651, 831, 978, 1398
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 3 cut(s) 230, 1311, 1573
HspAI GCGC 5 cut(s) 11, 21, 136, 584, 1648
KpnI GGTACC 1 cut(s) 1061
KspAI GTTAAC 1 cut(s) 697
Kzo9I GATC 4 cut(s) 433, 1097, 1303, 1495
LmnI GCTCC 4 cut(s) 248, 275, 316, 1528
Lsp1109I GCAGC 2 cut(s) 1507, 1609
LweI GCATC 5 cut(s) 625, 964, 1327, 1408, 1444
MaeI CTAG 3 cut(s) 456, 885, 1334
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 205
MalI GATC 4 cut(s) 435, 1099, 1305, 1497
MboI GATC 4 cut(s) 433, 1097, 1303, 1495
MfeI CAATTG 1 cut(s) 539
MhlI GDGCHC 3 cut(s) 234, 321, 1392
MlyI GAGTC 4 cut(s) 542, 614, 814, 1490
MmeI TCCRAC 1 cut(s) 270
Mph1103I ATGCAT 1 cut(s) 640
MseI TTAA 4 cut(s) 293, 696, 798, 816
MspA1I CMGCKG 1 cut(s) 1606
MspI CCGG 3 cut(s) 121, 688, 737
MspR9I CCNGG 1 cut(s) 738
MunI CAATTG 1 cut(s) 539
MvnI CGCG 3 cut(s) 13, 586, 991
MwoI GCNNNNNNNGC 5 cut(s) 391, 581, 1372, 1520, 1603
NciI CCSGG 1 cut(s) 738
NcoI CCATGG 1 cut(s) 1307
NdeII GATC 4 cut(s) 433, 1097, 1303, 1495
NlaIII CATG 3 cut(s) 230, 1311, 1573
NlaIV GGNNCC 3 cut(s) 686, 718, 1059
NsiI ATGCAT 1 cut(s) 640
NspV TTCGAA 1 cut(s) 644
PaeR7I CTCGAG 1 cut(s) 233
PfeI GAWTC 3 cut(s) 197, 844, 1193
PinAI ACCGGT 1 cut(s) 687
PkrI GCNGC 3 cut(s) 990, 1522, 1599
PleI GAGTC 4 cut(s) 541, 613, 814, 1489
PpsI GAGTC 4 cut(s) 541, 613, 814, 1489
Psp124BI GAGCTC 3 cut(s) 234, 321, 1392
PspFI CCCAGC 1 cut(s) 1613
PspN4I GGNNCC 3 cut(s) 686, 718, 1059
PspPI GGNCC 3 cut(s) 1202, 1444, 1512
PspXI VCTCGAGB 1 cut(s) 233
PsrI GAACNNNNNNTAC 2 cut(s) 886, 918
PvuII CAGCTG 1 cut(s) 1606
RsaI GTAC 4 cut(s) 102, 946, 1059, 1072
RsaNI GTAC 4 cut(s) 101, 945, 1058, 1071
SacI GAGCTC 3 cut(s) 234, 321, 1392
SaqAI TTAA 4 cut(s) 293, 696, 798, 816
SatI GCNGC 3 cut(s) 989, 1521, 1598
Sau3AI GATC 4 cut(s) 433, 1097, 1303, 1495
Sau96I GGNCC 3 cut(s) 1202, 1444, 1512
SchI GAGTC 4 cut(s) 542, 614, 814, 1490
ScrFI CCNGG 1 cut(s) 738
SduI GDGCHC 3 cut(s) 234, 321, 1392
SfaNI GCATC 5 cut(s) 625, 964, 1327, 1408, 1444
SfcI CTRYAG 1 cut(s) 395
Sfr274I CTCGAG 1 cut(s) 233
SfuI TTCGAA 1 cut(s) 644
SinI GGWCC 2 cut(s) 1202, 1444
SlaI CTCGAG 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
SsiI CCGC 7 cut(s) 5, 57, 68, 176, 314, 989, 1061
SspMI CTAG 3 cut(s) 456, 885, 1334
SstI GAGCTC 3 cut(s) 234, 321, 1392
StyD4I CCNGG 1 cut(s) 736
StyI CCWWGG 2 cut(s) 609, 1307
TaaI ACNGT 8 cut(s) 105, 355, 403, 1075, 1189, 1210, 1432, 1448
TaiI ACGT 1 cut(s) 261
TaqI TCGA 4 cut(s) 195, 234, 285, 644
TaqII GACCGA 1 cut(s) 264
TauI GCSGC 1 cut(s) 991
TfiI GAWTC 3 cut(s) 197, 844, 1193
Tru1I TTAA 4 cut(s) 293, 696, 798, 816
Tru9I TTAA 4 cut(s) 293, 696, 798, 816
TscAI CASTG 3 cut(s) 328, 1266, 1522
TseI GCWGC 2 cut(s) 1520, 1597
TspDTI ATGAA 5 cut(s) 456, 933, 1041, 1535, 1586
TspGWI ACGGA 2 cut(s) 82, 88
TspRI CASTG 3 cut(s) 328, 1266, 1522
VpaK11BI GGWCC 2 cut(s) 1202, 1444
XapI RAATTY 4 cut(s) 75, 144, 338, 1173
XhoI CTCGAG 1 cut(s) 233
XspI CTAG 3 cut(s) 456, 885, 1334
ZraI GACGTC 1 cut(s) 259
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.