Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Forward (+)

23121 .. 31227

Gene Structure 8107 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G01040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5730 bp

ATGGTAATGGAGGATGAGCCTAGAGAAGCCACAATAAAGCCTTCTTATTGGCTAGATGCTTGCGAGGACATCTCTTGTGATCTTATCGATGATCTCGTGTCTGAATTTGATCCTTCCTCTGTTGCTGTCAATGAATCCACTGATGAAAACGGCGTCATCAATGATTTTTTCGGTGGGATTGATCACATTTTAGATAGTATCAAGAACGGTGGAGGCTTACCAAACAATGGCGTTTCTGATACCAATTCTCAAATCAACGAGGTTACTGTAACTCCTCAGGTTATTGCTAAGGAGACAGTGAAGGAGAATGGGTTGCAAAAGAATGGCGGTAAGAGAGACGAATTCTCGAAAGAGGAAGGAGACAAGGATAGGAAGAGAGCTAGGGTTTGTAGTTATCAGAGTGAAAGGAGTAACCTTTCAGGTAGAGGGCATGTTAATAATTCTAGGGAGGGAGATAGGTTTATGAATAGGAAACGTACTCGTAATTGGGACGAGGCGGGTAACAATAAGAAGAAAAGGGAATGTAACAATTACAGAAGAGATGGTAGAGATAGAGAAGTTAGGGGTTATTGGGAGAGGGATAAAGTTGGTTCCAATGAGTTGGTTTATAGGTCAGGGACTTGGGAAGCTGATCATGAAAGAGATGTTAAGAAAGTGAGTGGTGGAAACCGCGAATGCGATGTCAAGGCAGAGGAGAACAAGAGTAAGCCTGAAGAACGTAAAGAGAAGGTTGTGGAAGAGCAAGCAAGGCGATACCAGTTGGATGTTCTTGAACAAGCTAAAGCGAAAAACACGATTGCTTTCCTTGAGACCGGTGCTGGAAAGACACTTATCGCGATTCTTCTTATTAAAAGTGTTCATAAGGATCTGATGAGCCAGAACAGAAAAATGCTCTCGGTGTTCTTGGTTCCCAAAGTGCCTTTGGTTTATCAGCAAGCAGAAGTGATCCGTAATCAAACTTGTTTTCAAGTTGGACATTATTGTGGTGAGATGGGACAGGACTTTTGGGATTCTCGAAGGTGGCAACGAGAGTTTGAGTCTAAGCAGGTTCTAGTTATGACAGCACAAATTCTGTTGAATATACTGAGACACAGTATCATTAGAATGGAAACAATTGATCTTCTTATTCTCGACGAGTGTCACCACGCTGTCAAGAAACATCCATACTCTTTAGTGATGTCAGAGTTTTACCATACAACTCCTAAAGATAAAAGACCTGCCATCTTTGGAATGACTGCTTCGCCTGTTAATTTAAAGGGTGTTTCAAGCCAAGTAGATTGTGCGATAAAGATACGTAACCTCGAGACCAAGTTGGATTCTACGGTTTGTACTATAAAAGATCGAAAAGAATTAGAGAAACATGTGCCTATGCCTTCAGAGATAGTCGTCGAGTATGACAAAGCTGCTACTATGTGGTCTCTTCATGAGACAATAAAGCAAATGATTGCAGCTGTTGAAGAAGCGGCACAAGCAAGTTCAAGGAAAAGCAAGTGGCAATTTATGGGGGCTAGGGATGCTGGAGCAAAGGATGAATTGAGACAGGTTTATGGCGTCTCTGAAAGAACGGAGAGCGATGGTGCTGCCAATTTGATTCATAAACTTAGAGCTATCAATTATACTCTTGCTGAATTGGGTCAATGGTGTGCTTACAAGGTGGGACAATCATTCTTGTCTGCTTTGCAAAGTGATGAGAGGGTGAATTTCCAAGTCGACGTGAAGTTTCAAGAATCATACCTCAGTGAGGTGGTGTCACTCTTGCAATGTGAGCTTCTGGAAGGCGCTGCTGCTGAAAAAGTCGCGGCGGAAGTTGGCAAACCAGAAAATGGTAATGCACATGACGAGATGGAGGAGGGAGAGCTCCCTGATGATCCTGTGGTCTCGGGAGGGGAGCACGTTGATGAAGTAATAGGCGCCGCAGTGGCTGATGGGAAAGTTACTCCAAAAGTACAATCATTGATCAAACTACTCCTCAAATATCAGCACACAGCTGATTTTCGAGCTATTGTTTTCGTTGAGAGGGTGGTTGCTGCTTTGGTTCTTCCTAAGGTTTTTGCGGAGCTGCCTTCGCTTAGTTTTATACGGTGTGCCAGCATGATTGGACACAATAACAGCCAGGAGATGAAATCATCTCAAATGCAGGATACAATTTCCAAATTCCGAGATGGGCATGTGACACTGTTAGTTGCCACAAGCGTTGCTGAGGAAGGACTTGATATTAGGCAATGTAACGTTGTTATGCGTTTCGACCTTGCAAAGACGGTGCTGGCATACATTCAGTCTCGTGGCCGGGCAAGAAAGCCTGGATCAGACTACATACTCATGGTTGAGAGAGGAAATGTATCTCACGCAGCGTTCCTAAGGAATGCTAGGAACAGTGAGGAGACACTTCGAAAAGAAGCAATAGAAAGGACTGATCTTAGTCATCTCAAAGATACATCGAGATTAATCTCAATTGATGCTGTGCCTGGTACAGTTTATAAGGTGGAGGCAACTGGTGCCATGGTTAGCTTGAATTCCGCGGTTGGTCTTGTACATTTCTACTGCTCTCAGCTTCCTGGTGACAGGTATGCAATCCTTCGTCCTGAGTTTAGCATGGAGAAGCATGAAAAGCCTGGGGGCCACACGGAATATTCATGTAGGCTTCAGCTTCCTTGCAATGCACCGTTTGAAATACTTGAGGGTCCTGTTTGCAGTTCAATGCGTCTTGCACAACAGGCTGTATGTTTAGCTGCTTGCAAGAAACTGCATGAGATGGGTGCATTTACCGATATGCTATTACCGGACAAAGGAAGTGGTCAAGACGCTGAGAAGGCTGACCAAGATGATGAAGGTGAGCCTGTTCCTGGAACTGCTAGACATAGAGAGTTCTATCCTGAAGGTGTGGCGGATGTACTTAAGGGAGAATGGGTTTCATCTGGAAAGGAAGTTTGTGAGAGCTCAAAGCTATTCCATTTATACATGTATAATGTCAGATGTGTAGATTTTGGCTCTTCAAAAGATCCATTCCTAAGCGAAGTTTCAGAGTTCGCGATTCTTTTTGGCAATGAGCTGGATGCAGAGGTATTATCGATGTCTATGGATCTTTATGTTGCTCGGGCCATGATCACTAAAGCATCTCTTGCTTTCAAGGGATCACTTGATATTACAGAAAACCAGCTATCATCTCTAAAAAAGTTTCATGTGAGGTTAATGAGTATCGTGTTGGATGTTGATGTTGAACCCTCCACGACACCATGGGATCCTGCAAAGGCCTACCTGTTTGTCCCTGTTACTGACAATACGTCTATGGAACCCATAAAAGGGATCAACTGGGAATTGGTTGAAAAGATTACGAAAACCACAGCGTGGGACAACCCTCTTCAGAGAGCTCGTCCCGATGTATATCTCGGGACTAATGAGAGAACTCTTGGTGGGGACAGAAGGGAATATGGGTTTGGTAAACTTCGTCACAACATTGTATTTGGGCAGAAATCTCACCCAACTTATGGTATTAGAGGAGCTGTTGCATCCTTCGATGTTGTGAGAGCTTCTGGATTGTTACCTGTGAGAGATGCTTTTGAGAAGGAAGTAGAAGAGGATTTATCAAAAGGAAAATTGATGATGGCTGATGGGTGCATGGTTGCAGAAGATCTTATTGGGAAAATAGTGACAGCCGCACATTCCGGGAAGCGGTTTTACGTAGATTCAATTTGTTATGACATGAGTGCAGAAACATCTTTCCCTAGGAAAGAGGGATATCTTGGTCCCCTAGAGTACAACACGTACGCTGACTATTACAAGCAAAAGTATGGAGTTGATTTGAACTGTAAGCAACAACCTTTGATTAAAGGACGTGGTGTTTCGTATTGCAAGAACCTTCTTTCTCCTCGGTTTGAACAGTCAGGTGAATCTGAGACAGTCCTTGATAAGACATATTACGTGTTTCTTCCACCTGAACTATGCGTTGTGCATCCGCTTTCGGGTTCACTTATCCGAGGTGCTCAGAGGTTACCCTCTATAATGAGAAGAGTTGAGAGCATGTTACTCGCTGTTCAACTCAAAAATTTGATTAGTTATCCTATTCCCACATCAAAGATTCTTGAAGCCTTGACTGCCGCCTCGTGCCAGGAAACGTTCTGCTACGAGAGAGCTGAGCTTTTAGGAGATGCGTATCTAAAATGGGTTGTTAGTCGTTTTCTGTTTCTCAAGTATCCTCAAAAGCACGAGGGTCAGCTTACAAGGATGAGGCAACAAATGGTTAGTAATATGGTTCTTTATCAGTTTGCTCTGGTTAAAGGGCTTCAGTCATATATCCAGGCGGATCGATTCGCCCCGTCTAGGTGGTCTGCTCCTGGTGTGCCTCCGGTTTTCGACGAGGACACAAAAGATGGAGGATCTTCGTTTTTCGATGAAGAGCAAAAACCTGTTTCCGAGGAAAACAGCGATGTGTTTGAAGATGGGGAGATGGAGGATGGTGAACTAGAGGGTGATTTGAGTTCGTACCGAGTTTTATCTAGCAAAACGTTAGCTGATGTTGTTGAGGCTTTGATTGGTGTTTATTACGTCGAAGGGGGTAAGATTGCAGCTAATCATTTGATGAAATGGATTGGGATTCACGTGGAGGATGATCCTGATGAAGTCGATGGAACATTGAAAAATGTTAATGTTCCAGAGAGTGTGCTCAAGAGCATCGACTTTGTTGGTCTTGAGAGAGCTCTTAAATATGAGTTTAAAGAGAAAGGTCTTCTTGTTGAAGCTATAACACATGCTTCAAGACCATCTTCAGGTGTTTCGTGTTACCAGAGATTGGAATTTGTTGGTGACGCGGTCTTGGATCATCTCATCACAAGACATCTATTTTTCACATACACAAGCCTTCCTCCTGGTCGGTTAACAGATCTTCGAGCTGCAGCGGTTAACAACGAGAATTTTGCTCGCGTTGCGGTTAAACATAAACTCCACTTGTACCTTCGTCACGGTTCAAGCGCCCTCGAAAAACAGATTCGGGAATTTGTGAAGGAGGTTCAAACCGAGTCATCGAAACCGGGGTTTAACTCTTTTGGTTTGGGAGACTGCAAAGCACCAAAAGTTCTTGGAGACATTGTTGAATCTATTGCAGGTGCTATTTTTCTTGATAGTGGAAAAGATACAACTGCTGCTTGGAAGGTTTTTCAACCTTTGCTTCAGCCCATGGTGACACCAGAGACACTTCCAATGCATCCGGTGCGAGAGCTACAAGAGCGGTGCCAGCAACAAGCAGAAGGGTTAGAATACAAAGCGAGTAGGAGTGGTAACACAGCGACTGTGGAAGTTTTCATCGACGGTGTTCAAGTTGGAGTAGCGCAAAACCCGCAGAAGAAAATGGCTCAAAAGCTAGCTGCGAGGAACGCACTTGCAGCTTTGAAAGAGAAAGAAATAGCAGAATCAAAGGAGAAGCATATCAACAACGGTAATGCGGGAGAGGATCAAGGCGAGAATGAGAATGGGAACAAGAAGAATGGGCATCAGCCGTTTACGAGACAAACGTTGAATGATATTTGTTTGAGGAAGAATTGGCCAATGCCTTCTTACAGATGTGTGAAAGAAGGAGGACCGGCTCATGCAAAGAGATTTACGTTTGGGGTAAGAGTTAATACGAGCGATAGAGGATGGACCGATGAGTGTATTGGCGAGCCAATGCCGAGTGTTAAGAAAGCTAAGGATTCAGCTGCGGTTCTTCTACTTGAGCTTTTAAATAAAACTTTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1909

Amino Acids

213.57

Weight (kDa)

5.88

Isoelectric Point (pI)

37.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	250 - 415	1.7e-14	Type III restriction enzyme, res subunit
DEAD	PF00270	251 - 415	3.4e-14	DEAD/DEAH box helicase
Helicase_C	PF00271	648 - 765	4.5e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	840 - 928	2.3e-21	Dicer dimerisation domain
PAZ	PF02170	1207 - 1337	7.2e-19	PAZ domain
Ribonucleas_3_3	PF14622	1362 - 1433	5.7e-08	Ribonuclease-III-like
Ribonuclease_3	PF00636	1375 - 1518	1.7e-20	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1573 - 1713	3.2e-15	Ribonuclease-III-like
Ribonuclease_3	PF00636	1594 - 1707	1.7e-23	Ribonuclease III domain
DND1_DSRM	PF14709	1832 - 1904	3.9e-16	double strand RNA binding domain from DEAD END PROTEIN 1
dsrm	PF00035	1834 - 1904	1.1e-08	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2478
AarI	CACCTGC	1 cut(s)	5087
Acc36I	ACCTGC	3 cut(s)	1036, 1225, 5087
AccB1I	GGYRCC	3 cut(s)	1910, 2495, 5223
AccB7I	CCANNNNNTGG	5 cut(s)	227, 1823, 3345, 3485, 4786
AccBSI	CCGCTC	1 cut(s)	5221
AccI	GTMKAC	1 cut(s)	1710
AccII	CGCG	7 cut(s)	672, 836, 1799, 2519, 3029, 4805, 4917
AclI	AACGTT	4 cut(s)	2229, 4112, 4502, 5504
AcoI	YGGCCR	2 cut(s)	2284, 5534
AcuI	CTGAAG	8 cut(s)	732, 1359, 2627, 2895, 3344, 4265, 4746, 5147
AcvI	CACGTG	1 cut(s)	4597
AcyI	GRCGYC	3 cut(s)	153, 1551, 1911
AdeI	CACNNNGTG	1 cut(s)	3345
AflII	CTTAAG	1 cut(s)	2894
AflIII	ACRYGT	4 cut(s)	1360, 2958, 3759, 3918
AgeI	ACCGGT	1 cut(s)	812
AhdI	GACNNNNNGTC	1 cut(s)	3280
AjiI	CACGTC	2 cut(s)	1714, 3833
AjuI	GAANNNNNNNTTGG	4 cut(s)	3417, 3449, 3618, 3650
Alw21I	GWGCWC	7 cut(s)	1860, 1893, 2939, 3370, 3982, 4662, 4696
AlwNI	CAGNNNCTG	2 cut(s)	1922, 5282
Ama87I	CYCGRG	4 cut(s)	1301, 1879, 3093, 3386
AoxI	GGCC	5 cut(s)	2284, 2617, 3096, 3249, 5534
ArsI	GACNNNNNNTTYG	4 cut(s)	666, 698, 987, 1019
AseI	ATTAAT	1 cut(s)	2444
AsiGI	ACCGGT	1 cut(s)	812
AspA2I	CCTAGG	1 cut(s)	3722
AspLEI	GCGC	4 cut(s)	1781, 1913, 4967, 5323
AspS9I	GGNCC	6 cut(s)	2617, 2681, 3096, 3743, 5570, 5631
AsuC2I	CCSGG	3 cut(s)	2288, 3664, 5025
AsuII	TTCGAA	1 cut(s)	2389
AsuNHI	GCTAGC	1 cut(s)	5353
AvaI	CYCGRG	4 cut(s)	1301, 1879, 3093, 3386
AvaII	GGWCC	4 cut(s)	2681, 3743, 5570, 5631
AvrII	CCTAGG	1 cut(s)	3722
AxyI	CCTNAGG	3 cut(s)	276, 2043, 2357
BalI	TGGCCA	1 cut(s)	5536
BamHI	GGATCC	1 cut(s)	3238
BanI	GGYRCC	3 cut(s)	1910, 2495, 5223
BanII	GRGCYC	4 cut(s)	1860, 2939, 3370, 4696
BarI	GAAGNNNNNNTAC	2 cut(s)	3659, 3691
BauI	CACGAG	4 cut(s)	95, 2280, 4099, 4202
BbrPI	CACGTG	1 cut(s)	4597
BbsI	GAAGAC	1 cut(s)	4715
Bbv12I	GWGCWC	7 cut(s)	1860, 1893, 2939, 3370, 3982, 4662, 4696
BbvCI	CCTCAGC	1 cut(s)	2199
BceAI	ACGGC	2 cut(s)	166, 5473
BcgI	CGANNNNNNTGC	6 cut(s)	1562, 1596, 4006, 4040, 4892, 4926
BciVI	GTATCC	2 cut(s)	2134, 4200
BclI	TGATCA	4 cut(s)	181, 631, 1956, 3102
BcnI	CCSGG	3 cut(s)	2288, 3664, 5025
BfmI	CTRYAG	1 cut(s)	4887
BfoI	RGCGCY	3 cut(s)	1782, 1914, 4968
BfrI	CTTAAG	1 cut(s)	2894
BfuAI	ACCTGC	3 cut(s)	1036, 1225, 5087
BfuI	GTATCC	2 cut(s)	2134, 4200
BglI	GCCNNNNNGGC	1 cut(s)	1919
BglII	AGATCT	2 cut(s)	3628, 4876
BlnI	CCTAGG	1 cut(s)	3722
BlpI	GCTNAGC	1 cut(s)	4131
Bme18I	GGWCC	4 cut(s)	2681, 3743, 5570, 5631
BmeRI	GACNNNNNGTC	1 cut(s)	3280
BmeT110I	CYCGRG	4 cut(s)	1301, 1879, 3093, 3386
BmgBI	CACGTC	2 cut(s)	1714, 3833
BmgT120I	GGNCC	6 cut(s)	2617, 2681, 3096, 3743, 5570, 5631
BmrI	ACTGGG	1 cut(s)	3319
BmtI	GCTAGC	1 cut(s)	5357
BmuI	ACTGGG	1 cut(s)	3319
BoxI	GACNNNNGTC	1 cut(s)	1137
BpiI	GAAGAC	1 cut(s)	4715
BplI	GAGNNNNNCTC	2 cut(s)	56, 88
BpmI	CTGGAG	1 cut(s)	1539
Bpu10I	CCTNAGC	4 cut(s)	288, 2199, 3008, 5676
Bpu1102I	GCTNAGC	1 cut(s)	4131
Bpu14I	TTCGAA	1 cut(s)	2389
BpuEI	CTTGAG	6 cut(s)	827, 2696, 4169, 4646, 4706, 5723
BpuMI	CCSGG	3 cut(s)	2288, 3664, 5025
Bsa29I	ATCGAT	3 cut(s)	87, 3068, 4303
BsaAI	YACGTR	5 cut(s)	1295, 3679, 3762, 3919, 4597
BsaBI	GATNNNNATC	2 cut(s)	3381, 4149
BsaHI	GRCGYC	3 cut(s)	153, 1551, 1911
BsaI	GGTCTC	4 cut(s)	803, 1298, 1422, 1882
BsaWI	WCCGGW	4 cut(s)	812, 2779, 4342, 5200
Bse118I	RCCGGY	2 cut(s)	812, 5572
Bse1I	ACTGG	3 cut(s)	757, 2497, 3314
Bse21I	CCTNAGG	3 cut(s)	276, 2043, 2357
Bse3DI	GCAATG	4 cut(s)	1766, 2228, 2662, 3049
Bse8I	GATNNNNATC	2 cut(s)	3381, 4149
BseCI	ATCGAT	3 cut(s)	87, 3068, 4303
BseJI	GATNNNNATC	2 cut(s)	3381, 4149
BseMI	GCAATG	4 cut(s)	1766, 2228, 2662, 3049
BseNI	ACTGG	3 cut(s)	757, 2497, 3314
BseRI	GAGGAG	7 cut(s)	264, 707, 1862, 1958, 2393, 3510, 3855
BsgI	GTGCAG	1 cut(s)	3726
Bsh1236I	CGCG	7 cut(s)	672, 836, 1799, 2519, 3029, 4805, 4917
BshFI	GGCC	5 cut(s)	2286, 2619, 3098, 3251, 5536
BshNI	GGYRCC	3 cut(s)	1910, 2495, 5223
BshTI	ACCGGT	1 cut(s)	812
BshVI	ATCGAT	3 cut(s)	87, 3068, 4303
BsiHKAI	GWGCWC	7 cut(s)	1860, 1893, 2939, 3370, 3982, 4662, 4696
BsiHKCI	CYCGRG	4 cut(s)	1301, 1879, 3093, 3386
BsiSI	CCGG	8 cut(s)	813, 2287, 2780, 3663, 4343, 5024, 5201, 5573
BsiWI	CGTACG	1 cut(s)	3762
BsmBI	CGTCTC	2 cut(s)	330, 1558
BsmI	GAATGC	2 cut(s)	680, 2368
BsnI	GGCC	5 cut(s)	2286, 2619, 3098, 3251, 5536
Bso31I	GGTCTC	4 cut(s)	803, 1298, 1422, 1882
BsoBI	CYCGRG	4 cut(s)	1301, 1879, 3093, 3386
Bsp119I	TTCGAA	1 cut(s)	2389
Bsp1286I	GDGCHC	7 cut(s)	1860, 1893, 2939, 3370, 3982, 4662, 4696
Bsp1407I	TGTACA	1 cut(s)	2530
Bsp1720I	GCTNAGC	1 cut(s)	4131
Bsp19I	CCATGG	3 cut(s)	2499, 3233, 5169
Bsp68I	TCGCGA	2 cut(s)	836, 3029
BspANI	GGCC	5 cut(s)	2286, 2619, 3098, 3251, 5536
BspDI	ATCGAT	3 cut(s)	87, 3068, 4303
BspFNI	CGCG	7 cut(s)	672, 836, 1799, 2519, 3029, 4805, 4917
BspHI	TCATGA	2 cut(s)	634, 1423
BspMAI	CTGCAG	1 cut(s)	4891
BspMI	ACCTGC	3 cut(s)	1036, 1225, 5087
BspOI	GCTAGC	1 cut(s)	5357
BspQI	GCTCTTC	3 cut(s)	732, 2995, 4386
BspT104I	TTCGAA	1 cut(s)	2389
BspT107I	GGYRCC	3 cut(s)	1910, 2495, 5223
BspTI	CTTAAG	1 cut(s)	2894
BspTNI	GGTCTC	4 cut(s)	803, 1298, 1422, 1882
BsrBI	CCGCTC	1 cut(s)	5221
BsrDI	GCAATG	4 cut(s)	1766, 2228, 2662, 3049
BsrFI	RCCGGY	2 cut(s)	812, 5572
BsrGI	TGTACA	1 cut(s)	2530
BsrI	ACTGG	3 cut(s)	757, 2497, 3314
BssAI	RCCGGY	2 cut(s)	812, 5572
BssNI	GRCGYC	3 cut(s)	153, 1551, 1911
BssSI	CACGAG	4 cut(s)	95, 2280, 4099, 4202
BssT1I	CCWWGG	4 cut(s)	2499, 3233, 3722, 5169
Bst2BI	CACGAG	4 cut(s)	95, 2280, 4099, 4202
Bst6I	CTCTTC	9 cut(s)	368, 532, 732, 1425, 2995, 3363, 3567, 4000, 4386
BstACI	GRCGYC	3 cut(s)	153, 1551, 1911
BstAFI	CTTAAG	1 cut(s)	2894
BstAPI	GCANNNNNTGC	3 cut(s)	2495, 3119, 5203
BstAUI	TGTACA	1 cut(s)	2530
BstBAI	YACGTR	5 cut(s)	1295, 3679, 3762, 3919, 4597
BstBI	TTCGAA	1 cut(s)	2389
BstDSI	CCRYGG	4 cut(s)	2499, 2517, 3233, 5169
BstEII	GGTNACC	1 cut(s)	3987
BstENI	CCTNNNNNAGG	1 cut(s)	1739
BstFNI	CGCG	7 cut(s)	672, 836, 1799, 2519, 3029, 4805, 4917
BstH2I	RGCGCY	3 cut(s)	1782, 1914, 4968
BstHHI	GCGC	4 cut(s)	1781, 1913, 4967, 5323
BstNSI	RCATGY	6 cut(s)	434, 1364, 2171, 2962, 4021, 4748
BstPAI	GACNNNNGTC	1 cut(s)	1137
BstPI	GGTNACC	1 cut(s)	3987
BstSFI	CTRYAG	1 cut(s)	4887
BstSNI	TACGTA	2 cut(s)	1295, 3679
BstUI	CGCG	7 cut(s)	672, 836, 1799, 2519, 3029, 4805, 4917
BstV2I	GAAGAC	1 cut(s)	4715
BstX2I	RGATCY	7 cut(s)	865, 2998, 3079, 3238, 3628, 4373, 4876
BstXI	CCANNNNNNTGG	1 cut(s)	601
BstYI	RGATCY	7 cut(s)	865, 2998, 3079, 3238, 3628, 4373, 4876
Bsu15I	ATCGAT	3 cut(s)	87, 3068, 4303
Bsu36I	CCTNAGG	3 cut(s)	276, 2043, 2357
BsuI	GTATCC	2 cut(s)	2134, 4200
BsuRI	GGCC	5 cut(s)	2286, 2619, 3098, 3251, 5536
BsuTUI	ATCGAT	3 cut(s)	87, 3068, 4303
BtgI	CCRYGG	4 cut(s)	2499, 2517, 3233, 5169
BtgZI	GCGATG	3 cut(s)	693, 1587, 4437
BtrI	CACGTC	2 cut(s)	1714, 3833
BtsI	GCAGTG	1 cut(s)	1923
BtsIMutI	CAGTG	6 cut(s)	138, 303, 1744, 1923, 2174, 2380
BtuMI	TCGCGA	2 cut(s)	836, 3029
BveI	ACCTGC	3 cut(s)	1036, 1225, 5087
CaiI	CAGNNNCTG	2 cut(s)	1922, 5282
CciI	TCATGA	2 cut(s)	634, 1423
CfoI	GCGC	4 cut(s)	1781, 1913, 4967, 5323
Cfr10I	RCCGGY	2 cut(s)	812, 5572
Cfr13I	GGNCC	6 cut(s)	2617, 2681, 3096, 3743, 5570, 5631
Cfr42I	CCGCGG	1 cut(s)	2520
ClaI	ATCGAT	3 cut(s)	87, 3068, 4303
CseI	GACGC	5 cut(s)	142, 1540, 2690, 2810, 4811
CspAI	ACCGGT	1 cut(s)	812
CspCI	CAANNNNNGTGG	6 cut(s)	190, 225, 2176, 2211, 2773, 2808
DinI	GGCGCC	1 cut(s)	1912
DraI	TTTAAA	3 cut(s)	1254, 4711, 5712
DraIII	CACNNNGTG	1 cut(s)	3345
DriI	GACNNNNNGTC	1 cut(s)	3280
EaeI	YGGCCR	2 cut(s)	2284, 5534
Eam1104I	CTCTTC	9 cut(s)	368, 532, 732, 1425, 2995, 3363, 3567, 4000, 4386
Eam1105I	GACNNNNNGTC	1 cut(s)	3280
EarI	CTCTTC	9 cut(s)	368, 532, 732, 1425, 2995, 3363, 3567, 4000, 4386
EciI	GGCGGA	3 cut(s)	1817, 2900, 4313
Ecl136II	GAGCTC	4 cut(s)	1858, 2937, 3368, 4694
Eco105I	TACGTA	2 cut(s)	1295, 3679
Eco130I	CCWWGG	4 cut(s)	2499, 3233, 3722, 5169
Eco147I	AGGCCT	1 cut(s)	3251
Eco24I	GRGCYC	4 cut(s)	1860, 2939, 3370, 4696
Eco31I	GGTCTC	4 cut(s)	803, 1298, 1422, 1882
Eco32I	GATATC	1 cut(s)	3737
Eco47I	GGWCC	4 cut(s)	2681, 3743, 5570, 5631
Eco53kI	GAGCTC	4 cut(s)	1858, 2937, 3368, 4694
Eco57I	CTGAAG	8 cut(s)	732, 1359, 2627, 2895, 3344, 4265, 4746, 5147
Eco72I	CACGTG	1 cut(s)	4597
Eco81I	CCTNAGG	3 cut(s)	276, 2043, 2357
Eco88I	CYCGRG	4 cut(s)	1301, 1879, 3093, 3386
Eco91I	GGTNACC	1 cut(s)	3987
EcoICRI	GAGCTC	4 cut(s)	1858, 2937, 3368, 4694
EcoNI	CCTNNNNNAGG	1 cut(s)	1739
EcoO109I	RGGNCCY	1 cut(s)	2681
EcoO65I	GGTNACC	1 cut(s)	3987
EcoRI	GAATTC	2 cut(s)	341, 2512
EcoRV	GATATC	1 cut(s)	3737
EcoT14I	CCWWGG	4 cut(s)	2499, 3233, 3722, 5169
EcoT22I	ATGCAT	1 cut(s)	5199
EcoT38I	GRGCYC	4 cut(s)	1860, 2939, 3370, 4696
EgeI	GGCGCC	1 cut(s)	1912
EheI	GGCGCC	1 cut(s)	1912
ErhI	CCWWGG	4 cut(s)	2499, 3233, 3722, 5169
Esp3I	CGTCTC	2 cut(s)	330, 1558
FalI	AAGNNNNNCTT	6 cut(s)	3102, 3134, 4710, 4742, 4744, 4776
FauI	CCCGC	3 cut(s)	490, 5337, 5428
FbaI	TGATCA	4 cut(s)	181, 631, 1956, 3102
FblI	GTMKAC	1 cut(s)	1710
FriOI	GRGCYC	4 cut(s)	1860, 2939, 3370, 4696
GlaI	GCGC	4 cut(s)	1780, 1912, 4966, 5322
GsuI	CTGGAG	1 cut(s)	1539
HaeII	RGCGCY	3 cut(s)	1782, 1914, 4968
HaeIII	GGCC	5 cut(s)	2286, 2619, 3098, 3251, 5536
HapII	CCGG	8 cut(s)	813, 2287, 2780, 3663, 4343, 5024, 5201, 5573
HgaI	GACGC	5 cut(s)	142, 1540, 2690, 2810, 4811
HhaI	GCGC	4 cut(s)	1781, 1913, 4967, 5323
Hin1I	GRCGYC	3 cut(s)	153, 1551, 1911
Hin6I	GCGC	4 cut(s)	1779, 1911, 4965, 5321
HinP1I	GCGC	4 cut(s)	1779, 1911, 4965, 5321
HincII	GTYRAC	3 cut(s)	1711, 4872, 4897
HindII	GTYRAC	3 cut(s)	1711, 4872, 4897
HpaI	GTTAAC	2 cut(s)	4872, 4897
HpaII	CCGG	8 cut(s)	813, 2287, 2780, 3663, 4343, 5024, 5201, 5573
Hpy166II	GTNNAC	7 cut(s)	1711, 3440, 3965, 4457, 4872, 4897, 5493
Hpy8I	GTNNAC	7 cut(s)	1711, 3440, 3965, 4457, 4872, 4897, 5493
Hpy99I	CGWCG	6 cut(s)	1136, 1391, 1715, 4355, 4547, 5303
Hsp92I	GRCGYC	3 cut(s)	153, 1551, 1911
HspAI	GCGC	4 cut(s)	1779, 1911, 4965, 5321
KasI	GGCGCC	1 cut(s)	1910
Ksp22I	TGATCA	4 cut(s)	181, 631, 1956, 3102
KspAI	GTTAAC	2 cut(s)	4872, 4897
KspI	CCGCGG	1 cut(s)	2520
LguI	GCTCTTC	3 cut(s)	732, 2995, 4386
LmnI	GCTCC	6 cut(s)	1520, 1863, 1888, 2056, 3497, 4333
MbiI	CCGCTC	1 cut(s)	5221
MfeI	CAATTG	2 cut(s)	1113, 2451
MflI	RGATCY	7 cut(s)	865, 2998, 3079, 3238, 3628, 4373, 4876
MhlI	GDGCHC	7 cut(s)	1860, 1893, 2939, 3370, 3982, 4662, 4696
MlsI	TGGCCA	1 cut(s)	5536
MluNI	TGGCCA	1 cut(s)	5536
Mly113I	GGCGCC	1 cut(s)	1911
MlyI	GAGTC	2 cut(s)	1046, 5021
MmeI	TCCRAC	5 cut(s)	741, 952, 1293, 3183, 5293
Mox20I	TGGCCA	1 cut(s)	5536
Mph1103I	ATGCAT	1 cut(s)	5199
MscI	TGGCCA	1 cut(s)	5536
MslI	CAYNNNNRTG	4 cut(s)	981, 1103, 1896, 2318
Msp20I	TGGCCA	1 cut(s)	5536
MspA1I	CMGCKG	5 cut(s)	1451, 1988, 2519, 4892, 5687
MspCI	CTTAAG	1 cut(s)	2894
MspI	CCGG	8 cut(s)	813, 2287, 2780, 3663, 4343, 5024, 5201, 5573
MunI	CAATTG	2 cut(s)	1113, 2451
Mva1269I	GAATGC	2 cut(s)	680, 2368
MvnI	CGCG	7 cut(s)	672, 836, 1799, 2519, 3029, 4805, 4917
NarI	GGCGCC	1 cut(s)	1911
NciI	CCSGG	3 cut(s)	2288, 3664, 5025
NcoI	CCATGG	3 cut(s)	2499, 3233, 5169
NheI	GCTAGC	1 cut(s)	5353
NmeAIII	GCCGAG	1 cut(s)	5685
NmuCI	GTSAC	9 cut(s)	1139, 1749, 2170, 2558, 3446, 3646, 4799, 4952, 5173
NruI	TCGCGA	2 cut(s)	836, 3029
NsiI	ATGCAT	1 cut(s)	5199
NspI	RCATGY	6 cut(s)	434, 1364, 2171, 2962, 4021, 4748
NspV	TTCGAA	1 cut(s)	2389
PaeR7I	CTCGAG	1 cut(s)	1301
PagI	TCATGA	2 cut(s)	634, 1423
PaqCI	CACCTGC	1 cut(s)	5087
PceI	AGGCCT	1 cut(s)	3251
PciI	ACATGT	2 cut(s)	1360, 2958
PciSI	GCTCTTC	3 cut(s)	732, 2995, 4386
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 5501
PctI	GAATGC	2 cut(s)	680, 2368
Pfl23II	CGTACG	1 cut(s)	3762
PflFI	GACNNNGTC	1 cut(s)	4805
PflMI	CCANNNNNTGG	5 cut(s)	227, 1823, 3345, 3485, 4786
PfoI	TCCNGGA	2 cut(s)	2842, 3662
PinAI	ACCGGT	1 cut(s)	812
PleI	GAGTC	2 cut(s)	1045, 5020
PluTI	GGCGCC	1 cut(s)	1914
PmaCI	CACGTG	1 cut(s)	4597
PmlI	CACGTG	1 cut(s)	4597
PpsI	GAGTC	2 cut(s)	1045, 5020
Ppu21I	YACGTR	5 cut(s)	1295, 3679, 3762, 3919, 4597
PpuMI	RGGWCCY	1 cut(s)	2681
PscI	ACATGT	2 cut(s)	1360, 2958
PshAI	GACNNNNGTC	1 cut(s)	1137
PshBI	ATTAAT	1 cut(s)	2444
PsiI	TTATAA	1 cut(s)	2478
Psp124BI	GAGCTC	4 cut(s)	1860, 2939, 3370, 4696
Psp1406I	AACGTT	4 cut(s)	2229, 4112, 4502, 5504
Psp5II	RGGWCCY	1 cut(s)	2681
PspCI	CACGTG	1 cut(s)	4597
PspEI	GGTNACC	1 cut(s)	3987
PspLI	CGTACG	1 cut(s)	3762
PspPI	GGNCC	6 cut(s)	2617, 2681, 3096, 3743, 5570, 5631
PspPPI	RGGWCCY	1 cut(s)	2681
PstI	CTGCAG	1 cut(s)	4891
PstNI	CAGNNNCTG	2 cut(s)	1922, 5282
PsuI	RGATCY	7 cut(s)	865, 2998, 3079, 3238, 3628, 4373, 4876
PsyI	GACNNNGTC	1 cut(s)	4805
PvuII	CAGCTG	3 cut(s)	1451, 1988, 5687
RruI	TCGCGA	2 cut(s)	836, 3029
RseI	CAYNNNNRTG	4 cut(s)	981, 1103, 1896, 2318
SacI	GAGCTC	4 cut(s)	1860, 2939, 3370, 4696
SacII	CCGCGG	1 cut(s)	2520
SalI	GTCGAC	1 cut(s)	1709
SapI	GCTCTTC	3 cut(s)	732, 2995, 4386
Sau96I	GGNCC	6 cut(s)	2617, 2681, 3096, 3743, 5570, 5631
SchI	GAGTC	2 cut(s)	1046, 5021
SduI	GDGCHC	7 cut(s)	1860, 1893, 2939, 3370, 3982, 4662, 4696
SfcI	CTRYAG	1 cut(s)	4887
SfoI	GGCGCC	1 cut(s)	1912
Sfr274I	CTCGAG	1 cut(s)	1301
Sfr303I	CCGCGG	1 cut(s)	2520
SfuI	TTCGAA	1 cut(s)	2389
SgrBI	CCGCGG	1 cut(s)	2520
SinI	GGWCC	4 cut(s)	2681, 3743, 5570, 5631
SlaI	CTCGAG	1 cut(s)	1301
SmiMI	CAYNNNNRTG	4 cut(s)	981, 1103, 1896, 2318
SmlI	CTYRAG	8 cut(s)	806, 1301, 2675, 2894, 4184, 4661, 4685, 5702
SmoI	CTYRAG	8 cut(s)	806, 1301, 2675, 2894, 4184, 4661, 4685, 5702
SnaBI	TACGTA	2 cut(s)	1295, 3679
SseBI	AGGCCT	1 cut(s)	3251
SspDI	GGCGCC	1 cut(s)	1910
SspI	AATATT	1 cut(s)	2630
SstI	GAGCTC	4 cut(s)	1860, 2939, 3370, 4696
StuI	AGGCCT	1 cut(s)	3251
StyI	CCWWGG	4 cut(s)	2499, 3233, 3722, 5169
TaqII	GACCGA	1 cut(s)	5648
TatI	WGTACW	5 cut(s)	1328, 1945, 2530, 2890, 3753
TauI	GCSGC	5 cut(s)	1466, 1802, 1916, 3656, 4097
TscAI	CASTG	6 cut(s)	145, 303, 1744, 1923, 2181, 2380
TseFI	GTSAC	9 cut(s)	1139, 1749, 2170, 2558, 3446, 3646, 4799, 4952, 5173
Tsp45I	GTSAC	9 cut(s)	1139, 1749, 2170, 2558, 3446, 3646, 4799, 4952, 5173
TspGWI	ACGGA	3 cut(s)	938, 1580, 2639
TspRI	CASTG	6 cut(s)	145, 303, 1744, 1923, 2181, 2380
Tth111I	GACNNNGTC	1 cut(s)	4805
Van91I	CCANNNNNTGG	5 cut(s)	227, 1823, 3345, 3485, 4786
Vha464I	CTTAAG	1 cut(s)	2894
VpaK11BI	GGWCC	4 cut(s)	2681, 3743, 5570, 5631
VspI	ATTAAT	1 cut(s)	2444
XagI	CCTNNNNNAGG	1 cut(s)	1739
XceI	RCATGY	6 cut(s)	434, 1364, 2171, 2962, 4021, 4748
XcmI	CCANNNNNNNNNTGG	1 cut(s)	919
XhoI	CTCGAG	1 cut(s)	1301
XmaJI	CCTAGG	1 cut(s)	3722
XmiI	GTMKAC	1 cut(s)	1710
Zsp2I	ATGCAT	1 cut(s)	5199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.