Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1C

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Reverse (-)

55206979 .. 55215840

Gene Structure 8862 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1CG281100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5874 bp

ATGGATACAGAGGCGGCTAGGGTTACAGGGGACAGTGGTGGCAATGGGGGAGAGGCGGTTCGGGCTTCTTACTGGCTGGATGCTTGTGAGGACATTGGGGATTTTGTGGACTTTTCCGAAAATTCAGGTGCTACGGCAGTGGTGGCGAGTGGGAATGGTTGCAATCAGGAGGAGGTGGGTGATTTTTTCGGCGGGATTGATCACATTCTTGACAGTATCAAAAGCGGAGCTGGATTGCCGGATGGGAAGGGGGATGGGGTAGTGAGCAGTGGCAATGCGGAGGAGGTTGGGGCGACTGGCGTTTCCAATGGACTGAGTGGTGAGAATGGTCAGAGTGGGGGTCGAAATTCTGAGGGTGCTAATGGAGATGGGAGAGTGGCGGTGGTGGTGGCGGCTTCTGCTGCTAAGCGTAATCACAATGGTTTGAGCAAGTATGAGAGTAATGGGAGGTGGAATCGGGGTGTGAGGGAAAGAGATGTGAGTGGTGAAGAGCGGTGCCCCAAGAGGGTTGCTGTAGATAATGGTAGGAATGAGAGGTATAGTTCAGGCAGAGTGCAATATCAGCTGAGGGAGAGGAATTCTGGTCGGAAGAGGCCTCGTGATCCTCGGGATGACATTGATAGGAGGGATAGGGATAGGGACAGAGATAGGGACAGGGATAGAGATCGGACTAGGAGAAGAGAGAGTTATGGTAGTAATAGGAGGGATAGTAGAGATTGGGAAGCAAAGGGCTATTGGGAGAGGGATAAATTGGGGTCAAATGAGCTTGTTTTTAGATTAGGAGCTTATGAACCTCATCAGAAGAAAGAGGAGAAAGTCGCTAATGACAAAATCAATGAAAAGGATGTGAAGAAATCTGAAGAGCTGAAGAAAGAAAAGATTCCTGAGGAACGAGCCAGACAGTATCAATTGGATGTTCTTGAACAGGCAAAGAAGAGCAATACGATAGCTTTTCTTGAAACTGGGGCTGGGAAGACACTCATTGCCATTCTCCTCATGCAAAGTGTCTGCAATGATTTGCAAAAGAAGAATAAAAAGATGCTTTCTGTGTTTCTAGTTCCTAAAGTTCCGCTTGTTTATCAGCAAGCAGAAGTTATTCGCGAGCGAACTGGTTTCCAAGTGGGCCATTATTGTGGTGAGATGGGTCAAGATTTTTGGGATACACGAAGATGGCAGCGTGAGTTTGATACTAAACAGGTTCTAGTAATGACAGCTCAAATTCTATTGAACATTCTAAGACACAGCATTATAAAGATGGAATCGATTAATCTATTGATTCTGGATGAGTGCCATCACGCTGTGAAGAAACATCCATACTCTTTAGTGATGTCCGAGTTCTATCATACAACCCCAAAAGAGAAGAGACCATCTGTATTTGGAATGACTGCTTCTCCTGTTAACTTAAAAGGTGTTTCCAATCAACTAGATTGTGCAATAAAGATTCGTAATCTTGAAAGTAAACTGGATTCCGTTGTTTGCACCATAAAGGACCGGAAGGACCTGGAAAAGCATGTTCCAATGCCCTCAGAAATTGTTGTTGAGTATGACAAAGCAGCCAGCTTATGGTCCTTTCACGAACAGCTAAAACAAATGGAAGTGGAAGTTGAAGAAGCTGCAAAGTCTAGCTCTAGAAGAAGTAAATGGCAGTTTATGGGAGCTAGAGATGCTGGGGCCAAGGAAGAGCTACGCCAAGTGTATGGTGTTTCAGAAAGAACAGAAAGTGATGGGGCTGTTAACCTAATTCAGAAGCTGAGGGCTATCAATTATGCACTTGGTGAACTGGGTCAGTGGTGCGCCTACAAGGTGGCACAATCATTCTTGACAGCTTTACAAAATGATGAGAGGGCAAACTACCAGCTTGATGTCAAGTTTCAAGAAACTTACCTGATCAGAGTTGTATCTCTTTTACAATGTCATCTGTCAGAGGGCGCTGCTTCTGACAAGGAAACAAAACTACCAGATTCAGAGAGTGGTGTTTCTCATGACGAAATTGAGGAAGGAGAGCTACCTGATAGTCATGTTGTTTCTGGTGGGGAGCATGTAGATGTGATAATCGGAGCTGCTGTAGCTGATGGGAAGGTGACGCCAAAAGTGCAGTCACTAATTAAAGTTCTTCTCAAGTATCAGCATACAGAAGATTTCCGTGCAATCATCTTTGTGGAGCGAGTTGTCTCCGCTTTAGTTCTTCCTAAGGTCTTTGCTGAGCTTCCATCTCTAGGTTTTATCGAGTGTGCAAGCTTGATTGGCCACAATAATAGTCAGGAAATGCGAAGTAGCCAAATGCAGGACACAATTGCCAAATTCAAGGATGGTCGTGTGACATTGTTAGTTGCAACTAGTGTTGCTGAGGAAGGACTTGATATTCGACAGTGCAATGTTGTTATTCGCTTCGACCTTGCCAAAACTGTTTTGGCTTATATTCAGTCTAGGGGCCGTGCTAGAAAACCTGGGTCAGATTATATCTTGATGGTGGAAAGGGCAAATCTGTCACATGAAGCATTCTTACGGAATGCTAGAAATAGCGAGGAGACTTTACGGAAAGAAGCAATAGAGAGGACTGACCTGAGTGATTTGAAGGATACTTCAAGGTTAATTTCAGTGGAAACAGCACCAGGTACAGTGTATCAGGTGGAATCAACTGGTGCTTTGGTGAGCTTAAATTCTGCTGTTGGACTAATCCATTTCTACTGCTCTCAGCTTCCGAGTGACAGGTACTCAATACTTCATCCTGAATTTGTTATGGTGAGGCATGAGAAGCAAGAAGGCCCGACTGAATATTCATGCAAGCTTCAACTCCCTTGTAATGCACCATTTGAGACACTTGAAGGCCCTGTGTGCAGTTCAATGCACCTTGCGCAACAGGCTGTTTGTTTGGCTGCTTGTAAGAAACTCCATGAGATGGGAGCATTTACCGACATGCTATTGCCCGACAGGGGAGTTGGGGAAGAAAAAGAAAAGGTTGACAAGAATGACGAAGGAGATCCACTTCCTGGAACTGCTAGGCACAGAGAGTTCTATCCCGAAGGAGTAGCTAATATTCTCCTGGGACAATGGATCTTAGCTGGAAGAGATCTTGGCAATGAGGCCAAATTGATTCATGTTTACATGTATTCTGTGAAATGTGTAGACATTGGCTCTTCAAAAGATCCTTTCTTGACTCAAGTTTCAGATTTTGCAATTCTGTTAGGCAATGAGCTGGATGCAGAGGTGTTATCGATGTCGATGGATCTATTTGTTGCTCGGACCATGACTACAAAGGCTTCCCTTGTCTTTAGGGGTTCAATTCGTATTACTGAAAGTCAGTTGGCATCCCTTAAAAGTTTCCATGTAAGATTGATGAGTATTGTACTGGATGTGGATGTTGAACCTTCCACCACTCCTTGGGATCCTGCAAAGGCTTATTTATTTGTCCCCGTGGTTAGCGATAAATGTGGAGATCCTATGAAAGAAATAAATTGGAATCTGGTTGAAAATATACTTGGTGCAGATGCGTGGAACAATCCCCTTCAAAGAGCTAGGCCCGATGTTTACCTTGGCACAAATGAGCGAACCCTAGGAGGTGACAGAAGGGAGTATGGTTTTGGTAAACTGCGTCATGGCATGGCTTTTGGGCAAAAATCCCATCCAACCTATGGTATCAGAGGAGCTGTGGCACAGTTTGATGTTGTTAAAGCTTCTGGAGTGATCCCTGATCGTGGTGCCTTTGAAATGCGAAAGGATGTGGATCTGCAGCAACGCAAGTTGATGATGGCAGATAGTTGTACCAATGTTGAAGATTTGGTAGGGAAAATTGTGACAGCTGCTCACTCGGGGAAGAGATTTTATGTTGATTCAATATGCTACGATATGACTGCAGAAAACTCTTTCCCGAGGAAAGAAGGTTATCTTGGCCCTCTGGAGTACAGCTCATATGCTGATTATTACAAGCAAAAGTATGGAGTTGAGTTGATGTATAAGAAACAACCTCTAATAAAAGGACGTGGTGTCTCATATTGTAAGAATCTCCTGTCCCCCCGGTTTGACCACATGGAAGAACTTGAAGGTGAATCAGAAGAGTCTCTGGACAAAACATACTACGTATTTCTCCCTCCTGAGCTTTGTTTAGTACACCCACTGTCTGGATCACTTGTCCGTGGAGCCCAAAGATTACCCTCAATAATGAGAAGGGTAGAAAGCATGCTGCTAGCGGTTGAACTTAAGGAGATAATAAATTATCCTGTCCCTGCTTTTAAGATTTTGGAAGCATTGACTGCTGCTTCATGCCAGGAGACATTTTGCTATGAAAGAGCTGAGCTTCTGGGTGATGCCTACCTCAAATGGGTTGTTAGTCGATATCTCTTTCTTAAATACCCTCAGAAACACGAAGGTCAGCTTACTAGGATGAGACAACAAATGGTCAGTAACATGGTTTTGTATCATTATGCATTGAAAAGAGGACTTCAATCATATATCCAAGCAGATCGCTTTGCTCCGTCTCGATGGGCGGCTCCTGGGGTGTTGCCCGTCTTTGATGAGTATACAAAGGATGAAGAATCATCTTTGTTTGACCAAGAGGATGTGACTCGACGGAAAACAGATGACCCGGTTGATGAATATGAGGATGATGAATTGGAGGATGGCGAGCTGGAGAGTGACTTGAGTTCTTATAGGGTCCTGTCAAGCAAGACACTTGCAGATGTTGTTGAAGCGCTTATTGGGGTTTATTATGTAGAAGGTGGGAAAAATGCTGCTAACCATCTCATGAAATGGGTTGGAATTGATGTAGAGTTTAATGCTGATGAGATAGAGAGCACAACAAGGCCATCTAATGTTCCAGATAGCATTCTTAGGAGCATCGACTTTGATGCGTTAGAGGGTGCTTTGAATATTAAATTTAGAGACAAGGGCCTCTTGGTGGAAGCCATTAGTCATGCTTCCCGACCATCTTCTGGAGTAGCCTGCTATCAGCGGTTGGAGTTTGTGGGTGATGCTGTCTTAGATCATCTCATCACAAAGCACTTGTTCTTCACATACACAAATCTGCCTCCAGGCAGGTTGACCGATCTTAGAGCTGCTGCTGTTAACAATGAAAATTTTGCACGTGTTGCTGTAAAACATAATCTCCATCTACACCTTCGACATGGATCCAGTGCTCTTGAAAGACAGATTCATGACTTTGTGAAGGAAGCTGCAAATGAATTAAGAAAGCCTGGGTTCAACTCATTTGGTTTGGGAGATTGTAAAGCTCCCAAAGTTCTTGGTGACATTATTGAATCTATTGCCGGTGCCATTTTTCTCGATAGTGGACGTGATACTGCAGTTGTTTGGAAGGTGTTTCAACCTCTGCTTCAACCAATGGTCACCCCAGAGACACTACCAATGCATCCGGTCCGTGAGCTGCAAGAAAGATGCCAGCAACAGGCTGAAGGCTTGGAATACAAAGCCAGTCGAAGTGGCAATTTGGCTACTGTTGAAGTCTTGATCGATGGTGTCAAGGTGGGAATTGCCCAGAATCCACAAAAGAAGATGGCACAGAAACTGGCTGCCAGGAATGCTCTTGCTGCTTTAAAAGATAAGGACACAGCTGAAGCAAAGGAGAGGCAGGAGAGGGAGGAGGAAGATAACGGGAAGAAGAAGAAGAATGGTTGCCAAACATTTACCAGGCAAACCTTGAATGACATATGCTTGCGTAAAAACTGGCCTATGCCATTCTACAGGTGTGTCAATGAGGGTGGCCCTGCACATGCAAAGAAGTTTACGTTTGCTGTACGCGTGAATACCACTGACAGGGGATGGATAGATGAATGCGTAGGAGAGCCTATGCCGAGTGTCAAGAAAGCCAAAGACTCGGCAGCAGTTCTTCTATTGGAATTATTGAATAAGTTATACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1957

Amino Acids

219.31

Weight (kDa)

6.03

Isoelectric Point (pI)

41.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	300 - 465	1.7e-14	Type III restriction enzyme, res subunit
DEAD	PF00270	301 - 465	9.1e-16	DEAD/DEAH box helicase
Helicase_C	PF00271	697 - 814	1.6e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	889 - 977	4.4e-22	Dicer dimerisation domain
PAZ	PF02170	1250 - 1390	2.3e-18	PAZ domain
Ribonucleas_3_3	PF14622	1417 - 1486	1.2e-07	Ribonuclease-III-like
Ribonuclease_3	PF00636	1428 - 1573	2.8e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1627 - 1767	1.4e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1649 - 1762	1.7e-22	Ribonuclease III domain
dsrm	PF00035	1789 - 1849	1e-05	Double-stranded RNA binding motif
DND1_DSRM	PF14709	1879 - 1952	1.7e-15	double strand RNA binding domain from DEAD END PROTEIN 1
dsrm	PF00035	1882 - 1952	2.4e-07	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1250
Acc16I	TGCGCA	1 cut(s)	2856
Acc36I	ACCTGC	1 cut(s)	5022
AccB1I	GGYRCC	3 cut(s)	495, 3698, 5264
AccB7I	CCANNNNNTGG	7 cut(s)	1563, 2899, 2990, 3381, 3632, 4118, 4928
AccBSI	CCGCTC	1 cut(s)	493
AccI	GTMKAC	2 cut(s)	3126, 4517
AccII	CGCG	2 cut(s)	1101, 5751
AcoI	YGGCCR	1 cut(s)	2245
AcsI	RAATTY	9 cut(s)	121, 346, 577, 1218, 2300, 2659, 2732, 4871, 5071
AcuI	CTGAAG	4 cut(s)	879, 887, 5424, 5586
AcvI	CACGTG	1 cut(s)	5081
AcyI	GRCGYC	1 cut(s)	2084
AdeI	CACNNNGTG	2 cut(s)	1300, 1775
AfaI	GTAC	7 cut(s)	2617, 2714, 3348, 3763, 3902, 4107, 5748
AfeI	AGCGCT	1 cut(s)	4689
AflII	CTTAAG	1 cut(s)	4196
AflIII	ACRYGT	3 cut(s)	3105, 5080, 5749
AhdI	GACNNNNNGTC	1 cut(s)	3983
AhlI	ACTAGT	1 cut(s)	2336
AjiI	CACGTC	2 cut(s)	3980, 5288
AjuI	GAANNNNNNNTTGG	6 cut(s)	3462, 3494, 3870, 3902, 4677, 4709
AloI	GAACNNNNNNTCC	2 cut(s)	42, 74
Alw21I	GWGCWC	2 cut(s)	4793, 5134
AlwNI	CAGNNNCTG	2 cut(s)	1750, 5518
Ama87I	CYCGRG	3 cut(s)	606, 3808, 3868
Aor51HI	AGCGCT	1 cut(s)	4689
ApoI	RAATTY	9 cut(s)	121, 346, 577, 1218, 2300, 2659, 2732, 4871, 5071
ArsI	GACNNNNNNTTYG	4 cut(s)	2081, 2113, 2346, 2378
AseI	ATTAAT	1 cut(s)	1266
Asp700I	GAANNNNTTC	2 cut(s)	879, 1095
AspA2I	CCTAGG	1 cut(s)	3553
AspLEI	GCGC	4 cut(s)	1796, 1931, 2857, 4690
AsuC2I	CCSGG	2 cut(s)	4015, 4583
AsuNHI	GCTAGC	1 cut(s)	4183
AvaI	CYCGRG	3 cut(s)	606, 3808, 3868
AvaII	GGWCC	6 cut(s)	1489, 1498, 1566, 3243, 4651, 5368
AvrII	CCTAGG	1 cut(s)	3553
AxyI	CCTNAGG	2 cut(s)	885, 2190
BaeGI	GKGCMC	1 cut(s)	500
BaeI	ACNNNNGTAYC	2 cut(s)	5283, 5316
BalI	TGGCCA	1 cut(s)	2247
BamHI	GGATCC	2 cut(s)	3385, 5123
BanI	GGYRCC	3 cut(s)	495, 3698, 5264
BanII	GRGCYC	1 cut(s)	4141
BarI	GAAGNNNNNNTAC	4 cut(s)	1989, 2021, 2487, 2519
BauI	CACGAG	1 cut(s)	597
BbrPI	CACGTG	1 cut(s)	5081
BbsI	GAAGAC	1 cut(s)	980
Bbv12I	GWGCWC	2 cut(s)	4793, 5134
BbvCI	CCTCAGC	3 cut(s)	566, 1751, 2346
BceAI	ACGGC	2 cut(s)	150, 2418
BcgI	CGANNNNNNTGC	4 cut(s)	3833, 3867, 4826, 4860
BciVI	GTATCC	2 cut(s)	1153, 2572
BclI	TGATCA	2 cut(s)	199, 1887
BcnI	CCSGG	2 cut(s)	4015, 4583
BcuI	ACTAGT	1 cut(s)	2336
BfmI	CTRYAG	6 cut(s)	513, 2064, 3728, 3852, 5295, 5692
BfoI	RGCGCY	2 cut(s)	1932, 4691
BfrI	CTTAAG	1 cut(s)	4196
BfuAI	ACCTGC	1 cut(s)	5022
BfuI	GTATCC	2 cut(s)	1153, 2572
BglII	AGATCT	1 cut(s)	3070
BlnI	CCTAGG	1 cut(s)	3553
BlpI	GCTNAGC	3 cut(s)	405, 2202, 4290
Bme18I	GGWCC	6 cut(s)	1489, 1498, 1566, 3243, 4651, 5368
BmeRI	GACNNNNNGTC	1 cut(s)	3983
BmeT110I	CYCGRG	3 cut(s)	606, 3808, 3868
BmgBI	CACGTC	2 cut(s)	3980, 5288
BmrI	ACTGGG	2 cut(s)	972, 1790
BmtI	GCTAGC	1 cut(s)	4187
BmuI	ACTGGG	2 cut(s)	972, 1790
BpiI	GAAGAC	1 cut(s)	980
BplI	GAGNNNNNCTC	2 cut(s)	3890, 3922
BpmI	CTGGAG	5 cut(s)	3699, 3917, 4646, 4950, 5010
Bpu10I	CCTNAGC	4 cut(s)	566, 1751, 2346, 4092
Bpu1102I	GCTNAGC	3 cut(s)	405, 2202, 4290
BpuEI	CTTGAG	3 cut(s)	2102, 3144, 4657
BpuMI	CCSGG	2 cut(s)	4015, 4583
Bsa29I	ATCGAT	3 cut(s)	1262, 3215, 5463
BsaAI	YACGTR	2 cut(s)	4078, 5081
BsaBI	GATNNNNATC	2 cut(s)	663, 3723
BsaHI	GRCGYC	1 cut(s)	2084
BsaI	GGTCTC	1 cut(s)	1357
BsaWI	WCCGGW	2 cut(s)	1491, 5365
Bse118I	RCCGGY	1 cut(s)	5261
Bse21I	CCTNAGG	2 cut(s)	885, 2190
Bse3DI	GCAATG	7 cut(s)	49, 280, 981, 1018, 2380, 3085, 3196
Bse8I	GATNNNNATC	2 cut(s)	663, 3723
BseCI	ATCGAT	3 cut(s)	1262, 3215, 5463
BseJI	GATNNNNATC	2 cut(s)	663, 3723
BseMI	GCAATG	7 cut(s)	49, 280, 981, 1018, 2380, 3085, 3196
BseRI	GAGGAG	7 cut(s)	185, 296, 824, 983, 2540, 3657, 5606
BseSI	GKGCMC	1 cut(s)	500
BseYI	CCCAGC	2 cut(s)	968, 1667
BsgI	GTGCAG	4 cut(s)	2114, 2857, 3504, 5703
Bsh1236I	CGCG	2 cut(s)	1101, 5751
BshNI	GGYRCC	3 cut(s)	495, 3698, 5264
BshVI	ATCGAT	3 cut(s)	1262, 3215, 5463
BsiHKAI	GWGCWC	2 cut(s)	4793, 5134
BsiHKCI	CYCGRG	3 cut(s)	606, 3808, 3868
BsiSI	CCGG	6 cut(s)	239, 1492, 4015, 4583, 5262, 5366
BslFI	GGGAC	7 cut(s)	44, 653, 665, 3060, 3395, 3994, 4205
BsmBI	CGTCTC	1 cut(s)	4479
BsmFI	GGGAC	7 cut(s)	44, 653, 665, 3060, 3395, 3994, 4205
BsmI	GAATGC	5 cut(s)	2498, 2515, 4821, 5536, 5789
Bso31I	GGTCTC	1 cut(s)	1357
BsoBI	CYCGRG	3 cut(s)	606, 3808, 3868
Bsp1286I	GDGCHC	4 cut(s)	500, 4141, 4793, 5134
Bsp1720I	GCTNAGC	3 cut(s)	405, 2202, 4290
Bsp68I	TCGCGA	1 cut(s)	1101
BspDI	ATCGAT	3 cut(s)	1262, 3215, 5463
BspFNI	CGCG	2 cut(s)	1101, 5751
BspHI	TCATGA	3 cut(s)	1981, 4740, 5149
BspMAI	CTGCAG	3 cut(s)	3732, 3856, 5299
BspMI	ACCTGC	1 cut(s)	5022
BspOI	GCTAGC	1 cut(s)	4187
BspQI	GCTCTTC	5 cut(s)	483, 855, 929, 1674, 3142
BspT107I	GGYRCC	3 cut(s)	495, 3698, 5264
BspTI	CTTAAG	1 cut(s)	4196
BspTNI	GGTCTC	1 cut(s)	1357
BsrBI	CCGCTC	1 cut(s)	493
BsrDI	GCAATG	7 cut(s)	49, 280, 981, 1018, 2380, 3085, 3196
BsrFI	RCCGGY	1 cut(s)	5261
BssAI	RCCGGY	1 cut(s)	5261
BssNAI	GTATAC	1 cut(s)	4518
BssNI	GRCGYC	1 cut(s)	2084
BssSI	CACGAG	1 cut(s)	597
BssT1I	CCWWGG	4 cut(s)	1674, 3380, 3532, 3553
Bst1107I	GTATAC	1 cut(s)	4518
Bst2BI	CACGAG	1 cut(s)	597
Bst4CI	ACNGT	9 cut(s)	35, 215, 903, 2370, 2407, 2620, 3657, 4116, 5449
BstACI	GRCGYC	1 cut(s)	2084
BstAFI	CTTAAG	1 cut(s)	4196
BstAPI	GCANNNNNTGC	2 cut(s)	4250, 5084
BstBAI	YACGTR	2 cut(s)	4078, 5081
BstDSI	CCRYGG	2 cut(s)	3414, 4132
BstEII	GGTNACC	1 cut(s)	5338
BstFNI	CGCG	2 cut(s)	1101, 5751
BstH2I	RGCGCY	2 cut(s)	1932, 4691
BstHHI	GCGC	4 cut(s)	1796, 1931, 2857, 4690
BstNSI	RCATGY	6 cut(s)	1514, 2042, 2920, 3109, 4180, 5726
BstPI	GGTNACC	1 cut(s)	5338
BstSFI	CTRYAG	6 cut(s)	513, 2064, 3728, 3852, 5295, 5692
BstSLI	GKGCMC	1 cut(s)	500
BstSNI	TACGTA	1 cut(s)	4078
BstUI	CGCG	2 cut(s)	1101, 5751
BstV2I	GAAGAC	1 cut(s)	980
BstX2I	RGATCY	9 cut(s)	2980, 3054, 3070, 3145, 3226, 3385, 3436, 3724, 5123
BstXI	CCANNNNNNTGG	2 cut(s)	1133, 1697
BstYI	RGATCY	9 cut(s)	2980, 3054, 3070, 3145, 3226, 3385, 3436, 3724, 5123
BstZ17I	GTATAC	1 cut(s)	4518
Bsu15I	ATCGAT	3 cut(s)	1262, 3215, 5463
Bsu36I	CCTNAGG	2 cut(s)	885, 2190
BsuI	GTATCC	2 cut(s)	1153, 2572
BsuTUI	ATCGAT	3 cut(s)	1262, 3215, 5463
BtgI	CCRYGG	2 cut(s)	3414, 4132
BtrI	CACGTC	2 cut(s)	3980, 5288
BtsI	GCAGTG	2 cut(s)	144, 274
BtuMI	TCGCGA	1 cut(s)	1101
BveI	ACCTGC	1 cut(s)	5022
CaiI	CAGNNNCTG	2 cut(s)	1750, 5518
CciI	TCATGA	3 cut(s)	1981, 4740, 5149
CfoI	GCGC	4 cut(s)	1796, 1931, 2857, 4690
Cfr10I	RCCGGY	1 cut(s)	5261
ClaI	ATCGAT	3 cut(s)	1262, 3215, 5463
CpoI	CGGWCCG	1 cut(s)	5368
CseI	GACGC	2 cut(s)	2092, 3581
CsiI	ACCWGGT	1 cut(s)	2611
Csp6I	GTAC	7 cut(s)	2616, 2713, 3347, 3762, 3901, 4106, 5747
CspCI	CAANNNNNGTGG	2 cut(s)	5692, 5727
CspI	CGGWCCG	1 cut(s)	5368
CviQI	GTAC	7 cut(s)	2616, 2713, 3347, 3762, 3901, 4106, 5747
DraI	TTTAAA	1 cut(s)	5547
DraIII	CACNNNGTG	2 cut(s)	1300, 1775
DriI	GACNNNNNGTC	1 cut(s)	3983
EaeI	YGGCCR	1 cut(s)	2245
Eam1105I	GACNNNNNGTC	1 cut(s)	3983
Eco105I	TACGTA	1 cut(s)	4078
Eco130I	CCWWGG	4 cut(s)	1674, 3380, 3532, 3553
Eco147I	AGGCCT	1 cut(s)	595
Eco24I	GRGCYC	1 cut(s)	4141
Eco31I	GGTCTC	1 cut(s)	1357
Eco32I	GATATC	1 cut(s)	4334
Eco47I	GGWCC	6 cut(s)	1489, 1498, 1566, 3243, 4651, 5368
Eco47III	AGCGCT	1 cut(s)	4689
Eco57I	CTGAAG	4 cut(s)	879, 887, 5424, 5586
Eco72I	CACGTG	1 cut(s)	5081
Eco81I	CCTNAGG	2 cut(s)	885, 2190
Eco88I	CYCGRG	3 cut(s)	606, 3808, 3868
Eco91I	GGTNACC	1 cut(s)	5338
EcoO109I	RGGNCCY	4 cut(s)	1498, 2828, 4651, 4885
EcoO65I	GGTNACC	1 cut(s)	5338
EcoRI	GAATTC	1 cut(s)	577
EcoRV	GATATC	1 cut(s)	4334
EcoT14I	CCWWGG	4 cut(s)	1674, 3380, 3532, 3553
EcoT22I	ATGCAT	2 cut(s)	4426, 5364
EcoT38I	GRGCYC	1 cut(s)	4141
ErhI	CCWWGG	4 cut(s)	1674, 3380, 3532, 3553
Esp3I	CGTCTC	1 cut(s)	4479
FalI	AAGNNNNNCTT	8 cut(s)	1056, 1088, 3870, 3902, 4356, 4388, 4874, 4906
FaqI	GGGAC	7 cut(s)	44, 653, 665, 3060, 3395, 3994, 4205
FauI	CCCGC	1 cut(s)	185
FauNDI	CATATG	2 cut(s)	3910, 5660
FbaI	TGATCA	2 cut(s)	199, 1887
FblI	GTMKAC	2 cut(s)	3126, 4517
FriOI	GRGCYC	1 cut(s)	4141
FspI	TGCGCA	1 cut(s)	2856
GlaI	GCGC	4 cut(s)	1795, 1930, 2856, 4689
GsaI	CCCAGC	2 cut(s)	972, 1671
GsuI	CTGGAG	5 cut(s)	3699, 3917, 4646, 4950, 5010
HaeII	RGCGCY	2 cut(s)	1932, 4691
HapII	CCGG	6 cut(s)	239, 1492, 4015, 4583, 5262, 5366
HgaI	GACGC	2 cut(s)	2092, 3581
HhaI	GCGC	4 cut(s)	1796, 1931, 2857, 4690
Hin1I	GRCGYC	1 cut(s)	2084
Hin6I	GCGC	4 cut(s)	1794, 1929, 2855, 4688
HinP1I	GCGC	4 cut(s)	1794, 1929, 2855, 4688
HincII	GTYRAC	5 cut(s)	1399, 1735, 2962, 5037, 5062
HindII	GTYRAC	5 cut(s)	1399, 1735, 2962, 5037, 5062
HindIII	AAGCTT	3 cut(s)	2236, 2786, 3672
HpaI	GTTAAC	3 cut(s)	1399, 1735, 5062
HpaII	CCGG	6 cut(s)	239, 1492, 4015, 4583, 5262, 5366
Hpy99I	CGWCG	1 cut(s)	4569
HpyCH4III	ACNGT	9 cut(s)	35, 215, 903, 2370, 2407, 2620, 3657, 4116, 5449
HpyCH4IV	ACGT	5 cut(s)	3979, 4077, 5080, 5287, 5738
HpySE526I	ACGT	5 cut(s)	3979, 4077, 5080, 5287, 5738
Hsp92I	GRCGYC	1 cut(s)	2084
HspAI	GCGC	4 cut(s)	1794, 1929, 2855, 4688
Ksp22I	TGATCA	2 cut(s)	199, 1887
KspAI	GTTAAC	3 cut(s)	1399, 1735, 5062
LguI	GCTCTTC	5 cut(s)	483, 855, 929, 1674, 3142
MabI	ACCWGGT	1 cut(s)	2611
MaeII	ACGT	5 cut(s)	3979, 4077, 5080, 5287, 5738
MbiI	CCGCTC	1 cut(s)	493
MfeI	CAATTG	2 cut(s)	908, 2292
MflI	RGATCY	9 cut(s)	2980, 3054, 3070, 3145, 3226, 3385, 3436, 3724, 5123
MhlI	GDGCHC	4 cut(s)	500, 4141, 4793, 5134
MlsI	TGGCCA	1 cut(s)	2247
MluI	ACGCGT	1 cut(s)	5749
MluNI	TGGCCA	1 cut(s)	2247
MlyI	GAGTC	4 cut(s)	3151, 4064, 4555, 5819
MmeI	TCCRAC	5 cut(s)	566, 2650, 3650, 4732, 4932
Mox20I	TGGCCA	1 cut(s)	2247
Mph1103I	ATGCAT	2 cut(s)	4426, 5364
MroXI	GAANNNNTTC	2 cut(s)	879, 1095
MscI	TGGCCA	1 cut(s)	2247
MslI	CAYNNNNRTG	4 cut(s)	1131, 1168, 2043, 2156
Msp20I	TGGCCA	1 cut(s)	2247
MspA1I	CMGCKG	4 cut(s)	565, 3800, 4948, 5564
MspCI	CTTAAG	1 cut(s)	4196
MspI	CCGG	6 cut(s)	239, 1492, 4015, 4583, 5262, 5366
MunI	CAATTG	2 cut(s)	908, 2292
Mva1269I	GAATGC	5 cut(s)	2498, 2515, 4821, 5536, 5789
MvnI	CGCG	2 cut(s)	1101, 5751
NciI	CCSGG	2 cut(s)	4015, 4583
NdeI	CATATG	2 cut(s)	3910, 5660
NheI	GCTAGC	1 cut(s)	4183
NmeAIII	GCCGAG	2 cut(s)	5807, 5829
NruI	TCGCGA	1 cut(s)	1101
NsbI	TGCGCA	1 cut(s)	2856
NsiI	ATGCAT	2 cut(s)	4426, 5364
NspI	RCATGY	6 cut(s)	1514, 2042, 2920, 3109, 4180, 5726
PaeI	GCATGC	1 cut(s)	4180
PagI	TCATGA	3 cut(s)	1981, 4740, 5149
PceI	AGGCCT	1 cut(s)	595
PciI	ACATGT	1 cut(s)	3105
PciSI	GCTCTTC	5 cut(s)	483, 855, 929, 1674, 3142
PctI	GAATGC	5 cut(s)	2498, 2515, 4821, 5536, 5789
PdmI	GAANNNNTTC	2 cut(s)	879, 1095
PflMI	CCANNNNNTGG	7 cut(s)	1563, 2899, 2990, 3381, 3632, 4118, 4928
PfoI	TCCNGGA	1 cut(s)	2989
PleI	GAGTC	4 cut(s)	3151, 4063, 4555, 5819
PmaCI	CACGTG	1 cut(s)	5081
PmlI	CACGTG	1 cut(s)	5081
PpsI	GAGTC	4 cut(s)	3151, 4063, 4555, 5819
Ppu21I	YACGTR	2 cut(s)	4078, 5081
PpuMI	RGGWCCY	2 cut(s)	1498, 4651
PscI	ACATGT	1 cut(s)	3105
PshBI	ATTAAT	1 cut(s)	1266
PsiI	TTATAA	1 cut(s)	1250
Psp5II	RGGWCCY	2 cut(s)	1498, 4651
PspCI	CACGTG	1 cut(s)	5081
PspEI	GGTNACC	1 cut(s)	5338
PspFI	CCCAGC	2 cut(s)	968, 1667
PspPPI	RGGWCCY	2 cut(s)	1498, 4651
PstI	CTGCAG	3 cut(s)	3732, 3856, 5299
PstNI	CAGNNNCTG	2 cut(s)	1750, 5518
PsuI	RGATCY	9 cut(s)	2980, 3054, 3070, 3145, 3226, 3385, 3436, 3724, 5123
PvuII	CAGCTG	3 cut(s)	565, 3800, 5564
RruI	TCGCGA	1 cut(s)	1101
RsaI	GTAC	7 cut(s)	2617, 2714, 3348, 3763, 3902, 4107, 5748
RsaNI	GTAC	7 cut(s)	2616, 2713, 3347, 3762, 3901, 4106, 5747
RseI	CAYNNNNRTG	4 cut(s)	1131, 1168, 2043, 2156
Rsr2I	CGGWCCG	1 cut(s)	5368
RsrII	CGGWCCG	1 cut(s)	5368
SapI	GCTCTTC	5 cut(s)	483, 855, 929, 1674, 3142
SchI	GAGTC	4 cut(s)	3151, 4064, 4555, 5819
SduI	GDGCHC	4 cut(s)	500, 4141, 4793, 5134
SexAI	ACCWGGT	1 cut(s)	2611
SfcI	CTRYAG	6 cut(s)	513, 2064, 3728, 3852, 5295, 5692
SinI	GGWCC	6 cut(s)	1489, 1498, 1566, 3243, 4651, 5368
SmiMI	CAYNNNNRTG	4 cut(s)	1131, 1168, 2043, 2156
SmlI	CTYRAG	4 cut(s)	2117, 3159, 4196, 4636
SmoI	CTYRAG	4 cut(s)	2117, 3159, 4196, 4636
SnaBI	TACGTA	1 cut(s)	4078
SpeI	ACTAGT	1 cut(s)	2336
SphI	GCATGC	1 cut(s)	4180
SseBI	AGGCCT	1 cut(s)	595
SspI	AATATT	3 cut(s)	2777, 3037, 4867
StuI	AGGCCT	1 cut(s)	595
StyI	CCWWGG	4 cut(s)	1674, 3380, 3532, 3553
TaaI	ACNGT	9 cut(s)	35, 215, 903, 2370, 2407, 2620, 3657, 4116, 5449
TaiI	ACGT	5 cut(s)	3982, 4080, 5083, 5290, 5741
TaqII	GACCGA	1 cut(s)	5054
TatI	WGTACW	3 cut(s)	3346, 3900, 4105
TauI	GCSGC	3 cut(s)	17, 395, 4487
TspGWI	ACGGA	8 cut(s)	1459, 2132, 2521, 2551, 4121, 4461, 4582, 5360
Van91I	CCANNNNNTGG	7 cut(s)	1563, 2899, 2990, 3381, 3632, 4118, 4928
Vha464I	CTTAAG	1 cut(s)	4196
VpaK11BI	GGWCC	6 cut(s)	1489, 1498, 1566, 3243, 4651, 5368
VspI	ATTAAT	1 cut(s)	1266
XapI	RAATTY	9 cut(s)	121, 346, 577, 1218, 2300, 2659, 2732, 4871, 5071
XbaI	TCTAGA	1 cut(s)	1628
XceI	RCATGY	6 cut(s)	1514, 2042, 2920, 3109, 4180, 5726
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2407, 3629
XmaJI	CCTAGG	1 cut(s)	3553
XmiI	GTMKAC	2 cut(s)	3126, 4517
XmnI	GAANNNNTTC	2 cut(s)	879, 1095
Zsp2I	ATGCAT	2 cut(s)	4426, 5364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.