Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Reverse (-)

53342837 .. 53352195

Gene Structure 9359 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ58624

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5859 bp

ATGGATACAGAGCCGGCTAGGGTTACAGGGGACAGTGGTGGCAATGGGGGAGAGGCGGTTCGGGCTTCTTACTGGCTGGATGCTTGTGAGGACATTGGGGATTTTGTGGACTTTTCCGAAAATTCGGGTGCTACGGTAGTGGTGGCGAGTGGGAACGGTTGCAATCAGGAGGAGGTGGGTGATTTTTTCGGCGGGATTGATCACATTCTTGACAGTATCAAAAGTGGAGCTGGATTGCCGGATGGGAAGGGGGATGGGGTAGTGAGCAGTGGCAATGCGGAGGAGGTTGGGGCGACTGGCATTTCCAATGGACTGAGTGGTGAGAATGGTCAGAGTGGGGGTCGAAATTCTGAAGGTGCTAATGGAGATGGGAGAGTGGCGGTGGTGGTGGCTTCTGCTGCTAAGCGTAATCACAATGGTTTGAGCAAGTATGAGAGTAATGGGAGGTGGAATCGGGGTGTGAGGGAAAGAGATGTGAGTGGTGAAGAGCGGTGCCCCAAGAGGGTTGCGGTAGATAATGGTAGGAATGAGAGGTATAGTTCAGGCAGAGTGCAATATCAGCTGAGGGAGAGGAATTCTGGTCGGAAGAGGACTCGTGATCCTTGGGATGACATTGATAGGAGGGACAGGGATAGGGACAGAGATAGAGATCGGACTAGGAGAAGAGAGAGTTATGGTAGTAATAGGAGGGATAGTAGAGATTGGGAAGCAAAGGGCTATTGGGAGAGGGATAAATTGGGGTCAAATGAGCTTGTTTTTAGATTAGGAGCTTATGAACCTCATCAGAAGAAAGAGGAGAAAGTCGCTAATGACAAAATCAATGAAAAGGATGTGAAGAAATCTGAAGAGCTGAAGAAAGAAAAGATTCCTGAGGAACGAGCCAGACAGTATCAATTGGATGTTCTTGAACAGGCAAAGAAGAGCAATACGATAGCTTTTCTTGAAACTGGGGCTGGGAAGACACTCATTGCCATTCTCCTCATGCAAAGTGTCTGCAATGATTTGCAAAAGAAGAATAAAAAGATGCTTTCTGTGTTTCTAGTTCCTAAAGTTCCGCTTGTTTATCAGCAAGCAGAAGTTATTCGCGAGCGAACTGGTTTCCAAGTGGGCCATTATTGTGGTGAGATGGGTCAAGATTTTTGGGATACACGAAGATGGCAGCGTGAGTTTGATACTAAACAGGTTCTAGTAATGACAGCTCAAATTCTATTGAACATTCTAAGACACAGCATTATAAAGATGGAATCGATTAATCTATTGATTCTGGATGAGTGCCATCACGCTGTGAAGAAACATCCATACTCTTTAGTGATGTCCGAGTTCTATCATACAACCCCAAAAGAGAAGAGACCATCTGTATTTGGAATGACTGCTTCTCCTGTTAACTTAAAAGGTGTTTCCAATCAACTAGATTGTGCAATAAAGATTCGTAATCTTGAAAGTAAACTGGATTCCGTTGTTTGCACCATAAAGGACCGGAAGGACCTGGAAAAGCATGTTCCAATGCCCTCAGAAATTGTTGTTGAGTATGACAAAGCAGCCAGCTTATGGTCCTTTCATGAACAGCTAAAACAAATGGAAGTGGAAGTTGAAGAAGCTGCAAAGTCTAGCTCTAGAAGAAGTAAATGGCAGTTTATGGGAGCTAGAGATGCTGGGGCCAAGGAAGAGCTACGCCAAGTGTATGGTGTTTCAGAAAGAACAGAAAGTGATGGGGCTGTTAACCTAATTCAGAAGCTGAGGGCTATCAATTATGCACTTGGTGAACTGGGTCAGTGGTGCGCCTACAAGGTGGCACAATCATTCTTGACAGCTTTACAAAATGATGAGAGGGCAAACTACCAGCTTGATGTCAAGTTTCAAGAAACTTACCTGATCAGAGTTGTATCTCTTTTACAATGTCATCTGTCAGAGGGCGCTGCTTCTGACAAGGAAACAAAACTACCAGATTCAGAGAGTGGCGTTTCTCATGACGAAATTGAGGAAGGAGAGCTACCTGATAGTCATGTTGTTTCTGGTGGGGAGCATGTAGATGTGATAATCGGAGCTGCTGTAGCTGATGGGAAGGTGACGCCAAAAGTGCAGTCACTAATTAAAGTTCTTCTCAAGTATCAGCATACAGAAGATTTCCGTGCAATCATCTTTGTGGAGCGAGTTGTCTCCGCTTTAGTTCTTCCTAAGGTCTTTGCTGAGCTTCCATCTCTAGGTTTTATCGAGTGTGCAAGCTTGATTGGCCACAATAATAGTCAGGAAATGCGAAGTAGCCAAATGCAGGACACAATTGCCAAATTCAAGGATGGTCGTGTGACATTGTTAGTTGCAACTAGTGTTGCTGAGGAAGGACTTGATATTCGACAGTGCAATGTAGTTATTCGCTTCGACCTTGCCAAAACTGTTTTGGCTTATATTCAGTCTAGGGGCCGTGCTAGAAAACCTGGCTCAGATTATATCTTGATGGTGGAAAGGGCAAATCTGTCACATGAAGCATTCTTACGGAATGCAAGAAATAGCGAGGAGACTTTACGGAAAGAAGCAATAGAGAGGACTGACCTGAGTGATTTGAAGGATACTTCAAGGTTAATTTCAGTGGAAACAGCACCAGGTACAGTGTATCAGGTGGAATCAACTGGTGCTTTGGTGAGCTTAAATTCTGCTGTTGGACTAATCCATTTCTACTGCTCTCAGCTTCCGAGTGACAGGTACTCAATACTTCATCCTGAATTTGTTATGGTGAGGCATGAGAAGCAAGAAGGCCCGACTGAATATTCATGCAAGCTTCAACTCCCTTGTAATGCACCATTTGAGACACTTGAAGGCCCTGTGTGCAGTTCAATGCACCTTGCGCAACAGGCTGTTTGTTTGGCTGCTTGTAAGAAACTCCATGAGATGGGAGCATTTACCGACATGCTATTGCCCGACAGGGGAGTTGGGGAAGAAAAAGAAAAGGTTGACAAGAATGACGAAGGAGATCCACTTCCTGGAACTGCTAGGCACAGAGAGTTCTATCCCGAAGGAGTAGCTAATATTCTCCAGGGAGAATGGATCTTAGCTGGAAGAGATCTTGGCAATGAGGCCAAATTGATTCATGTTTACATGTATTCTGTGAAATGTGTAGACATTGGCTCTTCAAAAGATCCTTTCTTGACTCAAGTTTCAGATTTTGCAATTCTGTTAGGCAATGAGCTGGATGCAGAGGTGTTATCGATGTCGATGGATCTATTTGTTGCTCGGACCATGACTACAAAGGCTTCCCTTGTCTTTAGGGGTTCAATTCGTATTACTGAAAGTCAGTTGGCATCCCTTAAAAGTTTCCATGTAAGATTGATGAGTATTGTACTGGATGTGGATGTTGAACCTTCCACCACTCCTTGGGATCCTGCAAAGGCTTATTTATTTGTCCCCGTGGTTAGCGATAAATGTGGAGATCCTATGAAAGAAATAAATTGGAATCTGGTTGAAAATATACTTGGTGCAGATGCGTGGAACAATCCCCTTCAAAGAGCTAGGCCCGATGTTTACCTTGGCACAAATGAGCGAACCCTAGGAGGTGACAGAAGGGAGTATGGTTTTGGTAAACTGCGTCATGGCATGGCTTTTGGGCAAAAATTCCATCCAACCTATGGTATCAGAGGAGCTGTGGCACAGTTTGATGTTGTTAAAGCTGCTGGAGTGATCCCTGATCGGGGTGCCTTTGAAATGCGAAAGGATGTGGATCTGCAGCAACGCAAGTTGATGATGGCAGATAGTTGTACCAATGTTGAAGATTTGGTAGGGAAAATTGTGACAGCTGCTCACTCGGGGAAGAGATTTTATGTTGATTCAATATGCTATGATATGACTGCAGAAAACTCTTTCCCGAGGAAAGAAGGTTATCTTGGCCCTCTGGAGTACAGCTCATATGCTGATTATTACAAGCAAAAGTATGGAGTTGAGTTGATGTATAAGAAACAACCTCTAATAAAAGGACGTGGTGTCTCATATTGTAAGAATCTCCTGTCCCCCCGGTTTGACCACATGGAAGAACTTGAAGGTGAATCAGAAGAGTCTCTGGACAAAACATACTATGTATTTCTCCCTCCTGAGCTTTGTTTAGTACACCCACTGTCTGGATCACTTGTCCGTGGAGCCCAAAGATTACCCTCAATAATGAGAAGGGTAGAAAGCATGCTGCTAGCGGTTGAACTTAAGGAGATAATAAATTATCCTGTCCCTGCTTTTAAGATTTTGGAAGCATTGACTGCTGCTTCATGCCAGGAGACATTTTGCTATGAAAGAGCTGAGCTTCTGGGTGATGCCTACCTCAAATGGGTTGTTAGTCGATATCTTTTTCTTAAATACCCTCAGAAACACGAAGGTCAGCTTACTAGGATGAGACAACAAATGGTCAGTAACATGGTTTTGTATCATTATGCATTGAAAAGAGGACTTCAATCATATATCCAAGCAGATCGCTTTGCTCCGTCTCGATGGGCGGCTCCTGGGGTATTGCCTGTCTTTGATGAGTATACAAAGGATGAAGAATCATCTTTGTTTGACCAAGAGGATGTGACTCGACGGAAAACAGATGACCCGGTTGATGAATATGAGGATGATGAATTGGAGGATGGCGAGCTGGAGAGTGACTTGAGTTCTTATAGGGTCCTGTCAAGCAAGACACTTGCAGATGTTGTTGAAGCGCTTATTGGGGTTTATTATGTAGAAGGTGGGAAAAATGCTGCTAACCATCTCATGAAATGGGTTGGAATTGATGTAGAGTTTAATGCTGATGAGATAGAGAGCACAACAAGGCCATCTAATGTTCCAGATAGCATTCTTAGGAGCATCGACTTTGATGCGTTAGAGGGTGCTTTGAATATTAAATTTAGAGACAAGGGCCTCTTGGTGGAAGCCATTAGTCATGCTTCCCGACCATCTTCTGGAGTAGCCTGCTATCAGCGGTTGGAGTTTGTGGGTGATGCTGTCTTAGATCATCTCATCACAAAGCACTTGTTCTTCACATACACAAATCTGCCTCCAGGCAGGTTGACCGATCTTAGAGCTGCTGCTGTTAACAATGAAAATTTTGCACGTGTTGCTGTAAAACATAATCTCCATCTACACCTTCGACATGGATCCAGTGCTCTTGAAAGACAGATTCATGACTTTGTGAAGGAAGCTGCAAATGAATTAACAAAGCCTGGGTTCAACTCATTTGGTTTGGGAGATTGTAAAGCTCCCAAAGTTCTTGGTGACATTATTGAATCTATTGCCGGTGCCATTTTTCTCGATGGTGGACGTGATACTGCAGTTGTTTGGAAGGTGTTTCAACCTCTGCTTCAACCAATGGTGACCCCAGAGACACTACCAATGCATCCGGTCCGTGAGCTGCAAGAAAGATGCCAGCAACAGGCTGAAGGCTTGGAATACAAAGCCAGTCGAAGTGGCAATTTGGCTACTGTTGAAGTCTTGATCGATGGTGTCAAGGTGGGAATTGCTCAGAATCCACAAAAGAAGATGGCACAGAAACTGGCTGCCAGGAATGCTCTTGCTGCTTTAAAAGATAAGGACACAGCTGAAGCAAAGGAGAGGCTGGAGAGGGAGGAGGAAGATAACGGGAAGAAGAAGAAGAATGGTAGCCAAACATTTACCAGGCAAACCTTGAATGACATATGCTTGCGTAAAAACTGGCCTATGCCATTCTACAGGTGTGTCAATGAGGGTGGCCCTGCACATGCAAAGAAGTTTACGTTTGCTGTACGCGTGAATACCACTGACAGGGGATGGATAGATGAATGCGTAGGAGAGCCTATGCCGAGTGTCAAGAAAGCCAAAGACTCGGCAGCAGTTCTTCTATTGGAATTATTGAATAAGTTATACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1952

Amino Acids

218.74

Weight (kDa)

5.95

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	295 - 460	1.7e-14	Type III restriction enzyme, res subunit
DEAD	PF00270	296 - 460	9.1e-16	DEAD/DEAH box helicase
Helicase_C	PF00271	692 - 809	1.6e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	884 - 972	4.4e-22	Dicer dimerisation domain
PAZ	PF02170	1245 - 1385	2.3e-18	PAZ domain
Ribonucleas_3_3	PF14622	1412 - 1481	1.2e-07	Ribonuclease-III-like
Ribonuclease_3	PF00636	1423 - 1568	2.8e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1622 - 1762	8.8e-14	Ribonuclease-III-like
Ribonuclease_3	PF00636	1644 - 1757	1.7e-22	Ribonuclease III domain
dsrm	PF00035	1784 - 1844	1e-05	Double-stranded RNA binding motif
DND1_DSRM	PF14709	1874 - 1947	1.7e-15	double strand RNA binding domain from DEAD END PROTEIN 1
dsrm	PF00035	1877 - 1947	2.4e-07	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1235
Acc16I	TGCGCA	1 cut(s)	2841
Acc36I	ACCTGC	1 cut(s)	5007
AccB1I	GGYRCC	3 cut(s)	492, 3683, 5249
AccB7I	CCANNNNNTGG	7 cut(s)	1548, 2884, 2975, 3366, 3617, 4103, 4913
AccBSI	CCGCTC	1 cut(s)	490
AccI	GTMKAC	2 cut(s)	3111, 4502
AccII	CGCG	2 cut(s)	1086, 5736
AcoI	YGGCCR	1 cut(s)	2230
AcuI	CTGAAG	5 cut(s)	372, 864, 872, 5409, 5571
AcvI	CACGTG	1 cut(s)	5066
AcyI	GRCGYC	1 cut(s)	2069
AdeI	CACNNNGTG	2 cut(s)	1285, 1760
AfaI	GTAC	7 cut(s)	2602, 2699, 3333, 3748, 3887, 4092, 5733
AfeI	AGCGCT	1 cut(s)	4674
AflII	CTTAAG	1 cut(s)	4181
AflIII	ACRYGT	3 cut(s)	3090, 5065, 5734
AhdI	GACNNNNNGTC	1 cut(s)	3968
AhlI	ACTAGT	1 cut(s)	2321
AjiI	CACGTC	2 cut(s)	3965, 5273
AjuI	GAANNNNNNNTTGG	6 cut(s)	3447, 3479, 3855, 3887, 4662, 4694
AloI	GAACNNNNNNTCC	2 cut(s)	42, 74
Alw21I	GWGCWC	2 cut(s)	4778, 5119
AlwNI	CAGNNNCTG	2 cut(s)	1735, 5503
Ama87I	CYCGRG	2 cut(s)	3793, 3853
Aor51HI	AGCGCT	1 cut(s)	4674
ArsI	GACNNNNNNTTYG	4 cut(s)	2066, 2098, 2331, 2363
AseI	ATTAAT	1 cut(s)	1251
Asp700I	GAANNNNTTC	2 cut(s)	864, 1080
AspA2I	CCTAGG	1 cut(s)	3538
AspLEI	GCGC	4 cut(s)	1781, 1916, 2842, 4675
AsuC2I	CCSGG	2 cut(s)	4000, 4568
AsuNHI	GCTAGC	1 cut(s)	4168
AvaI	CYCGRG	2 cut(s)	3793, 3853
AvaII	GGWCC	6 cut(s)	1474, 1483, 1551, 3228, 4636, 5353
AvrII	CCTAGG	1 cut(s)	3538
AxyI	CCTNAGG	2 cut(s)	870, 2175
BaeGI	GKGCMC	1 cut(s)	497
BaeI	ACNNNNGTAYC	2 cut(s)	5268, 5301
BalI	TGGCCA	1 cut(s)	2232
BamHI	GGATCC	2 cut(s)	3370, 5108
BanI	GGYRCC	3 cut(s)	492, 3683, 5249
BanII	GRGCYC	1 cut(s)	4126
BarI	GAAGNNNNNNTAC	4 cut(s)	1974, 2006, 2472, 2504
BauI	CACGAG	1 cut(s)	594
BbrPI	CACGTG	1 cut(s)	5066
BbsI	GAAGAC	1 cut(s)	965
Bbv12I	GWGCWC	2 cut(s)	4778, 5119
BbvCI	CCTCAGC	3 cut(s)	563, 1736, 2331
BceAI	ACGGC	1 cut(s)	2403
BcgI	CGANNNNNNTGC	2 cut(s)	4811, 4845
BciVI	GTATCC	2 cut(s)	1138, 2557
BclI	TGATCA	2 cut(s)	199, 1872
BcnI	CCSGG	2 cut(s)	4000, 4568
BcuI	ACTAGT	1 cut(s)	2321
BfmI	CTRYAG	5 cut(s)	2049, 3713, 3837, 5280, 5677
BfoI	RGCGCY	2 cut(s)	1917, 4676
BfrI	CTTAAG	1 cut(s)	4181
BfuAI	ACCTGC	1 cut(s)	5007
BfuI	GTATCC	2 cut(s)	1138, 2557
BglII	AGATCT	1 cut(s)	3055
BlnI	CCTAGG	1 cut(s)	3538
BlpI	GCTNAGC	3 cut(s)	402, 2187, 4275
Bme18I	GGWCC	6 cut(s)	1474, 1483, 1551, 3228, 4636, 5353
BmeRI	GACNNNNNGTC	1 cut(s)	3968
BmeT110I	CYCGRG	2 cut(s)	3793, 3853
BmgBI	CACGTC	2 cut(s)	3965, 5273
BmrI	ACTGGG	2 cut(s)	957, 1775
BmtI	GCTAGC	1 cut(s)	4172
BmuI	ACTGGG	2 cut(s)	957, 1775
BpiI	GAAGAC	1 cut(s)	965
BplI	GAGNNNNNCTC	2 cut(s)	3875, 3907
BpmI	CTGGAG	7 cut(s)	3011, 3684, 3902, 4631, 4935, 4995, 5588
Bpu10I	CCTNAGC	4 cut(s)	563, 1736, 2331, 4077
Bpu1102I	GCTNAGC	3 cut(s)	402, 2187, 4275
BpuEI	CTTGAG	3 cut(s)	2087, 3129, 4642
BpuMI	CCSGG	2 cut(s)	4000, 4568
Bsa29I	ATCGAT	3 cut(s)	1247, 3200, 5448
BsaAI	YACGTR	1 cut(s)	5066
BsaBI	GATNNNNATC	2 cut(s)	648, 3708
BsaHI	GRCGYC	1 cut(s)	2069
BsaI	GGTCTC	1 cut(s)	1342
BsaWI	WCCGGW	2 cut(s)	1476, 5350
Bse118I	RCCGGY	2 cut(s)	13, 5246
Bse21I	CCTNAGG	2 cut(s)	870, 2175
Bse3DI	GCAATG	7 cut(s)	49, 280, 966, 1003, 2365, 3070, 3181
Bse8I	GATNNNNATC	2 cut(s)	648, 3708
BseCI	ATCGAT	3 cut(s)	1247, 3200, 5448
BseJI	GATNNNNATC	2 cut(s)	648, 3708
BseMI	GCAATG	7 cut(s)	49, 280, 966, 1003, 2365, 3070, 3181
BseRI	GAGGAG	7 cut(s)	185, 296, 809, 968, 2525, 3642, 5591
BseSI	GKGCMC	1 cut(s)	497
BseYI	CCCAGC	2 cut(s)	953, 1652
BsgI	GTGCAG	4 cut(s)	2099, 2842, 3489, 5688
Bsh1236I	CGCG	2 cut(s)	1086, 5736
BshNI	GGYRCC	3 cut(s)	492, 3683, 5249
BshVI	ATCGAT	3 cut(s)	1247, 3200, 5448
BsiHKAI	GWGCWC	2 cut(s)	4778, 5119
BsiHKCI	CYCGRG	2 cut(s)	3793, 3853
BsiSI	CCGG	7 cut(s)	14, 239, 1477, 4000, 4568, 5247, 5351
BslFI	GGGAC	6 cut(s)	44, 638, 650, 3380, 3979, 4190
BsmBI	CGTCTC	1 cut(s)	4464
BsmFI	GGGAC	6 cut(s)	44, 638, 650, 3380, 3979, 4190
BsmI	GAATGC	5 cut(s)	2483, 2500, 4806, 5521, 5774
Bso31I	GGTCTC	1 cut(s)	1342
BsoBI	CYCGRG	2 cut(s)	3793, 3853
Bsp1286I	GDGCHC	4 cut(s)	497, 4126, 4778, 5119
Bsp1720I	GCTNAGC	3 cut(s)	402, 2187, 4275
Bsp68I	TCGCGA	1 cut(s)	1086
BspDI	ATCGAT	3 cut(s)	1247, 3200, 5448
BspFNI	CGCG	2 cut(s)	1086, 5736
BspHI	TCATGA	4 cut(s)	1558, 1966, 4725, 5134
BspMAI	CTGCAG	3 cut(s)	3717, 3841, 5284
BspMI	ACCTGC	1 cut(s)	5007
BspOI	GCTAGC	1 cut(s)	4172
BspQI	GCTCTTC	5 cut(s)	480, 840, 914, 1659, 3127
BspT107I	GGYRCC	3 cut(s)	492, 3683, 5249
BspTI	CTTAAG	1 cut(s)	4181
BspTNI	GGTCTC	1 cut(s)	1342
BsrBI	CCGCTC	1 cut(s)	490
BsrDI	GCAATG	7 cut(s)	49, 280, 966, 1003, 2365, 3070, 3181
BsrFI	RCCGGY	2 cut(s)	13, 5246
BssAI	RCCGGY	2 cut(s)	13, 5246
BssNAI	GTATAC	1 cut(s)	4503
BssNI	GRCGYC	1 cut(s)	2069
BssSI	CACGAG	1 cut(s)	594
BssT1I	CCWWGG	5 cut(s)	602, 1659, 3365, 3517, 3538
Bst1107I	GTATAC	1 cut(s)	4503
Bst2BI	CACGAG	1 cut(s)	594
BstACI	GRCGYC	1 cut(s)	2069
BstAFI	CTTAAG	1 cut(s)	4181
BstAPI	GCANNNNNTGC	2 cut(s)	4235, 5069
BstBAI	YACGTR	1 cut(s)	5066
BstDSI	CCRYGG	2 cut(s)	3399, 4117
BstEII	GGTNACC	1 cut(s)	5323
BstFNI	CGCG	2 cut(s)	1086, 5736
BstH2I	RGCGCY	2 cut(s)	1917, 4676
BstHHI	GCGC	4 cut(s)	1781, 1916, 2842, 4675
BstNSI	RCATGY	6 cut(s)	1499, 2027, 2905, 3094, 4165, 5711
BstPI	GGTNACC	1 cut(s)	5323
BstSFI	CTRYAG	5 cut(s)	2049, 3713, 3837, 5280, 5677
BstSLI	GKGCMC	1 cut(s)	497
BstUI	CGCG	2 cut(s)	1086, 5736
BstV2I	GAAGAC	1 cut(s)	965
BstX2I	RGATCY	9 cut(s)	2965, 3039, 3055, 3130, 3211, 3370, 3421, 3709, 5108
BstXI	CCANNNNNNTGG	2 cut(s)	1118, 1682
BstYI	RGATCY	9 cut(s)	2965, 3039, 3055, 3130, 3211, 3370, 3421, 3709, 5108
BstZ17I	GTATAC	1 cut(s)	4503
Bsu15I	ATCGAT	3 cut(s)	1247, 3200, 5448
Bsu36I	CCTNAGG	2 cut(s)	870, 2175
BsuI	GTATCC	2 cut(s)	1138, 2557
BsuTUI	ATCGAT	3 cut(s)	1247, 3200, 5448
BtgI	CCRYGG	2 cut(s)	3399, 4117
BtrI	CACGTC	2 cut(s)	3965, 5273
BtsI	GCAGTG	1 cut(s)	274
BtsIMutI	CAGTG	9 cut(s)	40, 274, 1778, 2360, 2589, 2610, 4097, 5119, 5745
BtuMI	TCGCGA	1 cut(s)	1086
BveI	ACCTGC	1 cut(s)	5007
CaiI	CAGNNNCTG	2 cut(s)	1735, 5503
CciI	TCATGA	4 cut(s)	1558, 1966, 4725, 5134
CfoI	GCGC	4 cut(s)	1781, 1916, 2842, 4675
Cfr10I	RCCGGY	2 cut(s)	13, 5246
ClaI	ATCGAT	3 cut(s)	1247, 3200, 5448
CpoI	CGGWCCG	1 cut(s)	5353
CseI	GACGC	2 cut(s)	2077, 3566
CsiI	ACCWGGT	1 cut(s)	2596
Csp6I	GTAC	7 cut(s)	2601, 2698, 3332, 3747, 3886, 4091, 5732
CspCI	CAANNNNNGTGG	2 cut(s)	5677, 5712
CspI	CGGWCCG	1 cut(s)	5353
CviQI	GTAC	7 cut(s)	2601, 2698, 3332, 3747, 3886, 4091, 5732
DraI	TTTAAA	1 cut(s)	5532
DraIII	CACNNNGTG	2 cut(s)	1285, 1760
DriI	GACNNNNNGTC	1 cut(s)	3968
EaeI	YGGCCR	1 cut(s)	2230
Eam1105I	GACNNNNNGTC	1 cut(s)	3968
Eco130I	CCWWGG	5 cut(s)	602, 1659, 3365, 3517, 3538
Eco24I	GRGCYC	1 cut(s)	4126
Eco31I	GGTCTC	1 cut(s)	1342
Eco32I	GATATC	1 cut(s)	4319
Eco47I	GGWCC	6 cut(s)	1474, 1483, 1551, 3228, 4636, 5353
Eco47III	AGCGCT	1 cut(s)	4674
Eco57I	CTGAAG	5 cut(s)	372, 864, 872, 5409, 5571
Eco72I	CACGTG	1 cut(s)	5066
Eco81I	CCTNAGG	2 cut(s)	870, 2175
Eco88I	CYCGRG	2 cut(s)	3793, 3853
Eco91I	GGTNACC	1 cut(s)	5323
EcoO109I	RGGNCCY	4 cut(s)	1483, 2813, 4636, 4870
EcoO65I	GGTNACC	1 cut(s)	5323
EcoRI	GAATTC	1 cut(s)	574
EcoRV	GATATC	1 cut(s)	4319
EcoT14I	CCWWGG	5 cut(s)	602, 1659, 3365, 3517, 3538
EcoT22I	ATGCAT	2 cut(s)	4411, 5349
EcoT38I	GRGCYC	1 cut(s)	4126
ErhI	CCWWGG	5 cut(s)	602, 1659, 3365, 3517, 3538
Esp3I	CGTCTC	1 cut(s)	4464
FalI	AAGNNNNNCTT	8 cut(s)	1041, 1073, 3855, 3887, 4341, 4373, 4859, 4891
FaqI	GGGAC	6 cut(s)	44, 638, 650, 3380, 3979, 4190
FauI	CCCGC	1 cut(s)	185
FauNDI	CATATG	2 cut(s)	3895, 5645
FbaI	TGATCA	2 cut(s)	199, 1872
FblI	GTMKAC	2 cut(s)	3111, 4502
FriOI	GRGCYC	1 cut(s)	4126
FspI	TGCGCA	1 cut(s)	2841
GlaI	GCGC	4 cut(s)	1780, 1915, 2841, 4674
GsaI	CCCAGC	2 cut(s)	957, 1656
GsuI	CTGGAG	7 cut(s)	3011, 3684, 3902, 4631, 4935, 4995, 5588
HaeII	RGCGCY	2 cut(s)	1917, 4676
HapII	CCGG	7 cut(s)	14, 239, 1477, 4000, 4568, 5247, 5351
HgaI	GACGC	2 cut(s)	2077, 3566
HhaI	GCGC	4 cut(s)	1781, 1916, 2842, 4675
Hin1I	GRCGYC	1 cut(s)	2069
Hin6I	GCGC	4 cut(s)	1779, 1914, 2840, 4673
HinP1I	GCGC	4 cut(s)	1779, 1914, 2840, 4673
HincII	GTYRAC	5 cut(s)	1384, 1720, 2947, 5022, 5047
HindII	GTYRAC	5 cut(s)	1384, 1720, 2947, 5022, 5047
HindIII	AAGCTT	2 cut(s)	2221, 2771
HpaI	GTTAAC	3 cut(s)	1384, 1720, 5047
HpaII	CCGG	7 cut(s)	14, 239, 1477, 4000, 4568, 5247, 5351
Hpy99I	CGWCG	1 cut(s)	4554
HpyCH4IV	ACGT	4 cut(s)	3964, 5065, 5272, 5723
HpySE526I	ACGT	4 cut(s)	3964, 5065, 5272, 5723
Hsp92I	GRCGYC	1 cut(s)	2069
HspAI	GCGC	4 cut(s)	1779, 1914, 2840, 4673
KroI	GCCGGC	1 cut(s)	13
KroNI	GCCGGC	1 cut(s)	15
Ksp22I	TGATCA	2 cut(s)	199, 1872
KspAI	GTTAAC	3 cut(s)	1384, 1720, 5047
LguI	GCTCTTC	5 cut(s)	480, 840, 914, 1659, 3127
MabI	ACCWGGT	1 cut(s)	2596
MaeII	ACGT	4 cut(s)	3964, 5065, 5272, 5723
MbiI	CCGCTC	1 cut(s)	490
MfeI	CAATTG	2 cut(s)	893, 2277
MflI	RGATCY	9 cut(s)	2965, 3039, 3055, 3130, 3211, 3370, 3421, 3709, 5108
MhlI	GDGCHC	4 cut(s)	497, 4126, 4778, 5119
MlsI	TGGCCA	1 cut(s)	2232
MluI	ACGCGT	1 cut(s)	5734
MluNI	TGGCCA	1 cut(s)	2232
MlyI	GAGTC	5 cut(s)	586, 3136, 4049, 4540, 5804
MmeI	TCCRAC	5 cut(s)	563, 2635, 3635, 4717, 4917
Mox20I	TGGCCA	1 cut(s)	2232
Mph1103I	ATGCAT	2 cut(s)	4411, 5349
MroNI	GCCGGC	1 cut(s)	13
MroXI	GAANNNNTTC	2 cut(s)	864, 1080
MscI	TGGCCA	1 cut(s)	2232
MslI	CAYNNNNRTG	4 cut(s)	1116, 1153, 2028, 2141
Msp20I	TGGCCA	1 cut(s)	2232
MspA1I	CMGCKG	4 cut(s)	562, 3785, 4933, 5549
MspCI	CTTAAG	1 cut(s)	4181
MspI	CCGG	7 cut(s)	14, 239, 1477, 4000, 4568, 5247, 5351
MunI	CAATTG	2 cut(s)	893, 2277
Mva1269I	GAATGC	5 cut(s)	2483, 2500, 4806, 5521, 5774
MvnI	CGCG	2 cut(s)	1086, 5736
NaeI	GCCGGC	1 cut(s)	15
NciI	CCSGG	2 cut(s)	4000, 4568
NdeI	CATATG	2 cut(s)	3895, 5645
NgoMIV	GCCGGC	1 cut(s)	13
NheI	GCTAGC	1 cut(s)	4168
NmeAIII	GCCGAG	2 cut(s)	5792, 5814
NruI	TCGCGA	1 cut(s)	1086
NsbI	TGCGCA	1 cut(s)	2841
NsiI	ATGCAT	2 cut(s)	4411, 5349
NspI	RCATGY	6 cut(s)	1499, 2027, 2905, 3094, 4165, 5711
PaeI	GCATGC	1 cut(s)	4165
PagI	TCATGA	4 cut(s)	1558, 1966, 4725, 5134
PciI	ACATGT	1 cut(s)	3090
PciSI	GCTCTTC	5 cut(s)	480, 840, 914, 1659, 3127
PctI	GAATGC	5 cut(s)	2483, 2500, 4806, 5521, 5774
PdiI	GCCGGC	1 cut(s)	15
PdmI	GAANNNNTTC	2 cut(s)	864, 1080
PflMI	CCANNNNNTGG	7 cut(s)	1548, 2884, 2975, 3366, 3617, 4103, 4913
PfoI	TCCNGGA	1 cut(s)	2974
PleI	GAGTC	5 cut(s)	586, 3136, 4048, 4540, 5804
PmaCI	CACGTG	1 cut(s)	5066
PmlI	CACGTG	1 cut(s)	5066
PpsI	GAGTC	5 cut(s)	586, 3136, 4048, 4540, 5804
Ppu21I	YACGTR	1 cut(s)	5066
PpuMI	RGGWCCY	2 cut(s)	1483, 4636
PscI	ACATGT	1 cut(s)	3090
PshBI	ATTAAT	1 cut(s)	1251
PsiI	TTATAA	1 cut(s)	1235
Psp5II	RGGWCCY	2 cut(s)	1483, 4636
PspCI	CACGTG	1 cut(s)	5066
PspEI	GGTNACC	1 cut(s)	5323
PspFI	CCCAGC	2 cut(s)	953, 1652
PspPPI	RGGWCCY	2 cut(s)	1483, 4636
PstI	CTGCAG	3 cut(s)	3717, 3841, 5284
PstNI	CAGNNNCTG	2 cut(s)	1735, 5503
PsuI	RGATCY	9 cut(s)	2965, 3039, 3055, 3130, 3211, 3370, 3421, 3709, 5108
PvuII	CAGCTG	3 cut(s)	562, 3785, 5549
RruI	TCGCGA	1 cut(s)	1086
RsaI	GTAC	7 cut(s)	2602, 2699, 3333, 3748, 3887, 4092, 5733
RsaNI	GTAC	7 cut(s)	2601, 2698, 3332, 3747, 3886, 4091, 5732
RseI	CAYNNNNRTG	4 cut(s)	1116, 1153, 2028, 2141
Rsr2I	CGGWCCG	1 cut(s)	5353
RsrII	CGGWCCG	1 cut(s)	5353
SapI	GCTCTTC	5 cut(s)	480, 840, 914, 1659, 3127
SchI	GAGTC	5 cut(s)	586, 3136, 4049, 4540, 5804
SduI	GDGCHC	4 cut(s)	497, 4126, 4778, 5119
SexAI	ACCWGGT	1 cut(s)	2596
SfcI	CTRYAG	5 cut(s)	2049, 3713, 3837, 5280, 5677
SinI	GGWCC	6 cut(s)	1474, 1483, 1551, 3228, 4636, 5353
SmiMI	CAYNNNNRTG	4 cut(s)	1116, 1153, 2028, 2141
SmlI	CTYRAG	4 cut(s)	2102, 3144, 4181, 4621
SmoI	CTYRAG	4 cut(s)	2102, 3144, 4181, 4621
SpeI	ACTAGT	1 cut(s)	2321
SphI	GCATGC	1 cut(s)	4165
SspI	AATATT	3 cut(s)	2762, 3022, 4852
StyI	CCWWGG	5 cut(s)	602, 1659, 3365, 3517, 3538
TaiI	ACGT	4 cut(s)	3967, 5068, 5275, 5726
TaqII	GACCGA	1 cut(s)	5039
TatI	WGTACW	3 cut(s)	3331, 3885, 4090
TauI	GCSGC	1 cut(s)	4472
TscAI	CASTG	9 cut(s)	40, 274, 1778, 2360, 2589, 2610, 4104, 5119, 5752
TspGWI	ACGGA	8 cut(s)	1444, 2117, 2506, 2536, 4106, 4446, 4567, 5345
TspRI	CASTG	9 cut(s)	40, 274, 1778, 2360, 2589, 2610, 4104, 5119, 5752
Van91I	CCANNNNNTGG	7 cut(s)	1548, 2884, 2975, 3366, 3617, 4103, 4913
Vha464I	CTTAAG	1 cut(s)	4181
VpaK11BI	GGWCC	6 cut(s)	1474, 1483, 1551, 3228, 4636, 5353
VspI	ATTAAT	1 cut(s)	1251
XbaI	TCTAGA	1 cut(s)	1613
XceI	RCATGY	6 cut(s)	1499, 2027, 2905, 3094, 4165, 5711
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2392, 3614
XmaJI	CCTAGG	1 cut(s)	3538
XmiI	GTMKAC	2 cut(s)	3111, 4502
XmnI	GAANNNNTTC	2 cut(s)	864, 1080
Zsp2I	ATGCAT	2 cut(s)	4411, 5349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.