Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Reverse (-)

53678053 .. 53686739

Gene Structure 8687 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G026540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5733 bp

ATGGATACAGAGGCGGCTAGGGTTACAGGGGACAGTGGTGGCAATGGGGGAGAGGCAGTTCGGGCTTCTTACTGGCTGGATGCTTGTGAGGACATTGGGGATTTTGTGGACTTTTCCGAAAATTCAGGTGCTACGGCAGTGGTGGCCAGTGGGAATGGTTGCAATCAGGAGGAGGTGGGTGATTTTTTCGGCGGGATTGATCACATTCTTGACAGTATCAAAAGCGGAGCTGGATTGCCGGATGGGAAGGGGGATGGGGTAGTGAGCAGTGGCAATGCGGAGGAGGTTGGGGCGACTGGCGTTTCCAATGGACTGAGTGGTGAGAATGGTCAGAGTGGGGGTCGAAATTCTGAGGGTGCTAATGGAGATGGGAGAGTGGCGGTGGTGGTGGCGGCTTCTGCTGCTAAGCGTAATCACAATGGTTTGAGCAAGTATGAGAGTAATGGGAGGTGGAATCGGGGTGTGAGGGAAAGAGATGTGAGTGGTGAAGAGCGGTGCCCCAAGAGGGTTGCTGTAGATAATGGTAGGAATGAGAGGTATAGTTCAGGCAGAGTGCAATATCAGCTGAGGGAGAGGAATTCTGGTCGGAAGAGGCCTCGTGATCCTCGGGATGACATTGATAGGAGGGATAGGGATAGGGACAGAGATAGGGACAGGGATAGAGATCGGACTAGGAGAAGAGAGAGTTATGGTAGTAATAGGAGGGATAGTAGAGATTGGGAAGCAAAGGGCTATTGGGAGAGGGATAAATTGGGGTCAAATGAGCTTGTTTTTAGATTAGGAGCTTATGAACCTCATCAGAAGAAAGAGGAGAAAGTCGCTAATGACAAAATCAATGAAAAGGATGTGAAGAAATCTGAAGAGCTGAAGAAAGAAAAGATTCCTGAGGAACGAGCCAGACAGTATCAATTGGATGTTCTTGAACAGGCAAAGAAGAGCAATACGATAGCTTTTCTTGAAACTGGGGCTGGGAAGACCTCATTGCCATTCTCCTCATGCAAACAAGCAGAAGTTATTCGCGAGCGAACTGGTTTCCAAGTGGGCCATTATTGTGGTGAGATGGGTCAAGATTTTTGGGATACACGAAGATGGCAGCGTGAGTTTGATACTAAACAGGTTCTAGTAATGACAGCTCAAATTCTATTGAACATTCTAAGACACAGCATTATAAAGATGGAATCGATTAATCTATTGATTCTGGATGAGTGCCATCACGCTGTGAAGAAACATCCATACTCTTTAGTGATGTCCGAGTTCTATCATACAACCCCAAAAGAGAAGAGACCATCTGTATTTGGAATGACTGCTTCTCCTGTTAACTTAAAAGGTGTTTCCAATCAACTAGATTGTGCAATAAAGATTCGTAATCTTGAAAGTAAACTGGATTCCGTTGTTTGCACCATAAAGGACCGGAAGGACCTGGAAAAGCATGTTCCAATGCCCTCAGAAATTGTTGTTGAGTATGACAAAGCAGCCAGCTTATGGTCCTTTCACGAACAGCTAAAACAAATGGAAGTGGAAGTTGAAGAAGCTGCAAAGTCTAGCTCTAGAAGAAGTAAATGGCAGTTTATGGGAGCTAGAGATGCTGGGGCCAAGGAAGAGCTACGCCAAGTGTATGGTGTTTCAGAAAGAACAGAAAGTGATGGGGCTGTTAACCTAATTCAGAAGCTGAGGGCTATCAATTATGCACTTGGTGAACTGGGTCAGTGGTGCGCCTACAAGGTGGCACAATCATTCTTGACAGCTTTACAAAATGATGAGAGGGCAAACTACCAGCTTGATGTCAAGTTTCAAGAAACTTACCTGATCAGAGTTGTATCTCTTTTACAATGTCATCTGTCAGAGGGCGCTGCTTCTGACAAGGAAACAAAACTACCAGATTCAGAGAGTGGTGTTTCTCATGACGAAATTGAGGAAGGAGAGCTACCTGATAGTCATGTTGTTTCTGGTGGGGAGCATGTAGATGTGATAATCGGAGCTGCTGTAGCTGATGGGAAGGTGACGCCAAAAGTGCAGTCACTAATTAAAGTTCTTCTCAAGTATCAGCATACAGAAGATTTCCGTGCAATCATCTTTGTGGAGCGAGTTGTCTCCGCTTTAGTTCTTCCTAAGGTCTTTGCTGAGCTTCCATCTCTAGGTTTTATCGAGTGTGCAAGCTTGATTGGCCACAATAATAGTCAGGAAATGCGAAGTAGCCAAATGCAGGACACAATTGCCAAATTCAAGGATGGTCGTGTGACATTGTTAGTTGCAACTAGTGTTGCTGAGGAAGGACTTGATATTCGACAGTGCAATGTTGTTATTCGCTTCGACCTTGCCAAAACTGTTTTGGCTTATATTCAGTCTAGGGGCCGTGCTAGAAAACCTGGGTCAGATTATATCTTGATGGTGGAAAGGGCAAATCTGTCACATGAAGCATTCTTACGGAATGCTAGAAATAGCGAGGAGACTTTACGGAAAGAAGCAATAGAGAGGACTGACCTGAGTGATTTGAAGGATACTTCAAGGTTAATTTCAGTGGAAACAGCACCAGGTACAGTGTATCAGGTGGAATCAACTGGTGCTTTGGTGAGCTTAAATTCTGCTGTTGGACTAATCCATTTCTACTGCTCTCAGCTTCCGAGTGACAGGTACTCAATACTTCATCCTGAATTTGTTATGGTGAGGCATGAGAAGCAAGAAGGCCCGACTGAATATTCATGCAAGCTTCAACTCCCTTGTAATGCACCATTTGAGACACTTGAAGGCCCTGCTGTTTGTTTGGCTGCTTGTAAGAAACTCCATGAGATGGGAGCATTTACCGACATGCTATTGCCCGACAGGGGAGTTGGGGAAGAAAAAGAAAAGGTTGACAAGAATGACGAAGGAGATCCACTTCCTGGAACTGCTAGGCACAGAGAGTTCTATCCCGAAGGAGTAGCTAATATTCTCCTGGGACAATGGATCTTAGCTGGAAGAGATCTTGGCAATGAGGCCAAATTGATTCATGTTTACATGTATTCTGTGAAATGTGTAGACATTGGCTCTTCAAAAGATCCTTTCTTGACTCAAGTTTCAGATTTTGCAATTCTGTTAGGCAATGAGCTGGATGCAGAGGTGTTATCGATGTCGATGGATCTATTTGTTGCTCGGACCATGACTACAAAGGCTTCCCTTGTCTTTAGGGGTTCAATTCGTATTACTGAAAGTCAGTTGGCATCCCTTAAAAGTTTCCATGTAAGATTGATGAGTATTGTACTGGATGTGGATGTTGAACCTTCCACCACTCCTTGGGATCCTGCAAAGGCTTATTTATTTGTCCCCGTGGTTAGCGATAAATGTGGAGATCCTATGAAAGAAATAAATTGGAATCTGGTTGAAAATATACTTGGTGCAGATGCGTGGAACAATCCCCTTCAAAGAGCTAGGCCCGATGTTTACCTTGGCACAAATGAGCGAACCCTAGGAGGTGACAGAAGGGAGTATGGTTTTGGTAAACTGCGTCATGGCATGGCTTTTGGGCAAAAATCCCATCCAACCTATGGTATCAGAGGAGCTGTGGCACAGTTTGATGTTGTTAAAGCTTCTGGAGTGATCCCTGATCGTGGTGCCTTTGAAATGCGAAAGGATGTGGATCTGCAGCAACGCAAGTTGATGATGGCAGATAGTTGTACCAATGTTGAAGATTTGGTAGGGAAAATTGTGACAGCTGCTCACTCGGGGAAGAGATTTTATGTTGATTCAATATGCTACGATATGACTGCAGAAAACTCTTTCCCGAGGAAAGAAGGTTATCTTGGCCCTCTGGAGTACAGCTCATATGCTGATTATTACAAGCAAAAGTATGGAGTTGAGTTGATGTATAAGAAACAACCTCTAATAAAAGGACGTGGTGTCTCATATTGTAAGAATCTCCTGTCCCCCCGGTTTGACCACATGGAAGAACTTGAAGGTGAATCAGAAGAGTCTCTGGACAAAACATACTACGTATTTCTCCCTCCTGAGCTTTGTTTAGTACACCCACTGTCTGGATCACTTGTCCGTGGAGCCCAAAGATTACCCTCAATAATGAGAAGGGTAGAAAGCATGCTGCTAGCGGTTGAACTTAAGGAGATAATAAATTATCCTGTCCCTGCTTTTAAGATTTTGGAAGCATTGACTGCTGCTTCATGCCAGGAGACATTTTGCTATGAAAGAGCTGAGCTTCTGGGTGATGCCTACCTCAAATGGGTTGTTAGTCGATATCTCTTTCTTAAATACCCTCAGAAACACGAAGGTCAGCTTACTAGGATGAGACAACAAATGGTCAGTAACATGGTTTTGTATCATTATGCATTGAAAAGAGGACTTCAATCATATATCCAAGCAGATCGCTTTGCTCCGTCTCGATGGGCGGCTCCTGGGGTGTTGCCCGTCTTTGATGAGTATACAAAGGATGAAGAATCATCTTTGTTTGACCAAGAGGATGTGACTCGACGGAAAACAGATGACCCGGTTGATGAATATGAGGATGATGAATTGGAGGATGGCGAGCTGGAGAGTGACTTGAGTTCTTATAGGGTCCTGTCAAGCAAGACACTTGCAGATGTTGTTGAAGCGCTTATTGGGGTTTATTATGTAGAAGGTGGGAAAAATGCTGCTAACCATCTCATGAAATGGGTTGGAATTGATGTAGAGTTTAATGCTGATGAGATAGAGAGCACAACAAGGCCATCTAATGTTCCAGATAGCATTCTTAGGAGCATCGACTTTGATGCGTTAGAGGGTGCTTTGAATATTAAATTTAGAGACAAGGGCCTCTTGGTGGAAGCCATTAGTCATGCTTCCCGACCATCTTCTGGAGTAGCCTGCTATCAGCGGTTGGAGTTTGTGGGTGATGCTGTCTTAGATCATCTCATCACAAAGCACTTGTTCTTCACATACACAAATCTGCCTCCAGGCAGGTTGACCGATCTTAGAGCTGCTGCTGTTAACAATGAAAATTTTGCACGTGTTGCTGTAAAACATAATCTCCATCTACACCTTCGACATGGATCCAGTGCTCTTGAAAGACAGATTCATGACTTTGTGAAGGAAGCTGCAAATGAATTAACAAAGCCTGGGTTCAACTCATTTGGTTTGGGAGATTGTAAAGCTCCCAAAGTTCTTGGTGACATTATTGAATCTATTGCCGGTGCCATTTTTCTCGATAGTGGACGTGATACTGCAGTTGTTTGGAAGGTGTTTCAACCTCTGCTTCAACCAATGGTCACCCCAGAGACACTACCAATGCATCCGGTCCGTGAGCTGCAAGAAAGATGCCAGCAACAGGCTGAAGGCTTGGAATACAAAGCCAGTCGAAGTGGCAATTTGGCTACTGTTGAAGTCTTGATCGATGGTGTCAAGATGGCACAGAAACTGGCTGCCAGGAATGCTCTTGCTGCTTTAAAAGATAAGGACACAGCTGAAGCAAAGGAGAGGCAGGAGAGGGAGGAGGAAGATAACGGGAAGAAGAAGAAGAATGGTTGCCAAACATTTACCAGGCAAACCTTGAATGACATATGCTTGCGTAAAAACTGGCCTATGCCATTCTACAGGTGTGTCAATGAGGGTGGCCCTGCACATGCAAAGAAGTTTACGTTTGCTGTACGCGTGAATACCACTGACAGGGGATGGATAGATGAATGCGTAGGAGAGCCTATGCCGAGTGTCAAGAAAGCCAAAGACTCGGCAGCAGTTCTTCTATTGGAATTATTGAATAAGTTATACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1910

Amino Acids

213.95

Weight (kDa)

5.87

Isoelectric Point (pI)

41.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	670 - 787	1.6e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	862 - 940	1.3e-12	Dicer dimerisation domain
PAZ	PF02170	1213 - 1353	2.2e-18	PAZ domain
Ribonucleas_3_3	PF14622	1380 - 1449	1.1e-07	Ribonuclease-III-like
Ribonuclease_3	PF00636	1391 - 1536	2.8e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1590 - 1730	1.5e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1612 - 1725	1.7e-22	Ribonuclease III domain
DND1_DSRM	PF14709	1832 - 1905	1.6e-15	double strand RNA binding domain from DEAD END PROTEIN 1
dsrm	PF00035	1835 - 1905	2.4e-07	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1169
Acc36I	ACCTGC	1 cut(s)	4911
AccB1I	GGYRCC	3 cut(s)	495, 3587, 5153
AccB7I	CCANNNNNTGG	7 cut(s)	1482, 2788, 2879, 3270, 3521, 4007, 4817
AccBSI	CCGCTC	1 cut(s)	493
AccI	GTMKAC	2 cut(s)	3015, 4406
AccII	CGCG	2 cut(s)	1020, 5610
AcoI	YGGCCR	2 cut(s)	144, 2164
AcsI	RAATTY	9 cut(s)	121, 346, 577, 1137, 2219, 2578, 2651, 4760, 4960
AcuI	CTGAAG	4 cut(s)	879, 887, 5313, 5445
AcvI	CACGTG	1 cut(s)	4970
AcyI	GRCGYC	1 cut(s)	2003
AdeI	CACNNNGTG	2 cut(s)	1219, 1694
AfaI	GTAC	7 cut(s)	2536, 2633, 3237, 3652, 3791, 3996, 5607
AfeI	AGCGCT	1 cut(s)	4578
AflII	CTTAAG	1 cut(s)	4085
AflIII	ACRYGT	3 cut(s)	2994, 4969, 5608
AhdI	GACNNNNNGTC	1 cut(s)	3872
AhlI	ACTAGT	1 cut(s)	2255
AjiI	CACGTC	2 cut(s)	3869, 5177
AjuI	GAANNNNNNNTTGG	6 cut(s)	3351, 3383, 3759, 3791, 4566, 4598
AloI	GAACNNNNNNTCC	2 cut(s)	42, 74
Alw21I	GWGCWC	2 cut(s)	4682, 5023
AlwNI	CAGNNNCTG	2 cut(s)	1669, 5377
Ama87I	CYCGRG	3 cut(s)	606, 3697, 3757
Aor51HI	AGCGCT	1 cut(s)	4578
ApoI	RAATTY	9 cut(s)	121, 346, 577, 1137, 2219, 2578, 2651, 4760, 4960
ArsI	GACNNNNNNTTYG	4 cut(s)	2000, 2032, 2265, 2297
AseI	ATTAAT	1 cut(s)	1185
Asp700I	GAANNNNTTC	2 cut(s)	879, 1014
AspA2I	CCTAGG	1 cut(s)	3442
AspLEI	GCGC	3 cut(s)	1715, 1850, 4579
AsuC2I	CCSGG	2 cut(s)	3904, 4472
AsuNHI	GCTAGC	1 cut(s)	4072
AvaI	CYCGRG	3 cut(s)	606, 3697, 3757
AvaII	GGWCC	6 cut(s)	1408, 1417, 1485, 3132, 4540, 5257
AvrII	CCTAGG	1 cut(s)	3442
AxyI	CCTNAGG	2 cut(s)	885, 2109
BaeGI	GKGCMC	1 cut(s)	500
BaeI	ACNNNNGTAYC	2 cut(s)	5172, 5205
BalI	TGGCCA	2 cut(s)	146, 2166
BamHI	GGATCC	2 cut(s)	3274, 5012
BanI	GGYRCC	3 cut(s)	495, 3587, 5153
BanII	GRGCYC	1 cut(s)	4030
BarI	GAAGNNNNNNTAC	4 cut(s)	1908, 1940, 2406, 2438
BauI	CACGAG	1 cut(s)	597
BbrPI	CACGTG	1 cut(s)	4970
BbsI	GAAGAC	1 cut(s)	980
Bbv12I	GWGCWC	2 cut(s)	4682, 5023
BbvCI	CCTCAGC	3 cut(s)	566, 1670, 2265
BceAI	ACGGC	2 cut(s)	150, 2337
BcgI	CGANNNNNNTGC	4 cut(s)	3722, 3756, 4715, 4749
BciVI	GTATCC	2 cut(s)	1072, 2491
BclI	TGATCA	2 cut(s)	199, 1806
BcnI	CCSGG	2 cut(s)	3904, 4472
BcuI	ACTAGT	1 cut(s)	2255
BfmI	CTRYAG	6 cut(s)	513, 1983, 3617, 3741, 5184, 5551
BfoI	RGCGCY	2 cut(s)	1851, 4580
BfrI	CTTAAG	1 cut(s)	4085
BfuAI	ACCTGC	1 cut(s)	4911
BfuI	GTATCC	2 cut(s)	1072, 2491
BglII	AGATCT	1 cut(s)	2959
BlnI	CCTAGG	1 cut(s)	3442
BlpI	GCTNAGC	3 cut(s)	405, 2121, 4179
Bme18I	GGWCC	6 cut(s)	1408, 1417, 1485, 3132, 4540, 5257
BmeRI	GACNNNNNGTC	1 cut(s)	3872
BmeT110I	CYCGRG	3 cut(s)	606, 3697, 3757
BmgBI	CACGTC	2 cut(s)	3869, 5177
BmrI	ACTGGG	2 cut(s)	972, 1709
BmtI	GCTAGC	1 cut(s)	4076
BmuI	ACTGGG	2 cut(s)	972, 1709
BpiI	GAAGAC	1 cut(s)	980
BplI	GAGNNNNNCTC	2 cut(s)	3779, 3811
BpmI	CTGGAG	5 cut(s)	3588, 3806, 4535, 4839, 4899
Bpu10I	CCTNAGC	4 cut(s)	566, 1670, 2265, 3981
Bpu1102I	GCTNAGC	3 cut(s)	405, 2121, 4179
BpuEI	CTTGAG	3 cut(s)	2021, 3033, 4546
BpuMI	CCSGG	2 cut(s)	3904, 4472
Bsa29I	ATCGAT	3 cut(s)	1181, 3104, 5352
BsaAI	YACGTR	2 cut(s)	3967, 4970
BsaBI	GATNNNNATC	2 cut(s)	663, 3612
BsaHI	GRCGYC	1 cut(s)	2003
BsaI	GGTCTC	1 cut(s)	1276
BsaWI	WCCGGW	2 cut(s)	1410, 5254
Bse118I	RCCGGY	1 cut(s)	5150
Bse21I	CCTNAGG	2 cut(s)	885, 2109
Bse3DI	GCAATG	6 cut(s)	49, 280, 980, 2299, 2974, 3085
Bse8I	GATNNNNATC	2 cut(s)	663, 3612
BseCI	ATCGAT	3 cut(s)	1181, 3104, 5352
BseJI	GATNNNNATC	2 cut(s)	663, 3612
BseMI	GCAATG	6 cut(s)	49, 280, 980, 2299, 2974, 3085
BseRI	GAGGAG	7 cut(s)	185, 296, 824, 982, 2459, 3546, 5465
BseSI	GKGCMC	1 cut(s)	500
BseYI	CCCAGC	2 cut(s)	968, 1586
BsgI	GTGCAG	3 cut(s)	2033, 3393, 5562
Bsh1236I	CGCG	2 cut(s)	1020, 5610
BshNI	GGYRCC	3 cut(s)	495, 3587, 5153
BshVI	ATCGAT	3 cut(s)	1181, 3104, 5352
BsiHKAI	GWGCWC	2 cut(s)	4682, 5023
BsiHKCI	CYCGRG	3 cut(s)	606, 3697, 3757
BsiSI	CCGG	6 cut(s)	239, 1411, 3904, 4472, 5151, 5255
BslFI	GGGAC	7 cut(s)	44, 653, 665, 2949, 3284, 3883, 4094
BsmBI	CGTCTC	1 cut(s)	4368
BsmFI	GGGAC	7 cut(s)	44, 653, 665, 2949, 3284, 3883, 4094
BsmI	GAATGC	5 cut(s)	2417, 2434, 4710, 5395, 5648
Bso31I	GGTCTC	1 cut(s)	1276
BsoBI	CYCGRG	3 cut(s)	606, 3697, 3757
Bsp1286I	GDGCHC	4 cut(s)	500, 4030, 4682, 5023
Bsp1720I	GCTNAGC	3 cut(s)	405, 2121, 4179
Bsp68I	TCGCGA	1 cut(s)	1020
BspDI	ATCGAT	3 cut(s)	1181, 3104, 5352
BspFNI	CGCG	2 cut(s)	1020, 5610
BspHI	TCATGA	3 cut(s)	1900, 4629, 5038
BspMAI	CTGCAG	3 cut(s)	3621, 3745, 5188
BspMI	ACCTGC	1 cut(s)	4911
BspOI	GCTAGC	1 cut(s)	4076
BspQI	GCTCTTC	5 cut(s)	483, 855, 929, 1593, 3031
BspT107I	GGYRCC	3 cut(s)	495, 3587, 5153
BspTI	CTTAAG	1 cut(s)	4085
BspTNI	GGTCTC	1 cut(s)	1276
BsrBI	CCGCTC	1 cut(s)	493
BsrDI	GCAATG	6 cut(s)	49, 280, 980, 2299, 2974, 3085
BsrFI	RCCGGY	1 cut(s)	5150
BssAI	RCCGGY	1 cut(s)	5150
BssNAI	GTATAC	1 cut(s)	4407
BssNI	GRCGYC	1 cut(s)	2003
BssSI	CACGAG	1 cut(s)	597
BssT1I	CCWWGG	4 cut(s)	1593, 3269, 3421, 3442
Bst1107I	GTATAC	1 cut(s)	4407
Bst2BI	CACGAG	1 cut(s)	597
Bst4CI	ACNGT	9 cut(s)	35, 215, 903, 2289, 2326, 2539, 3546, 4005, 5338
BstACI	GRCGYC	1 cut(s)	2003
BstAFI	CTTAAG	1 cut(s)	4085
BstAPI	GCANNNNNTGC	2 cut(s)	4139, 4973
BstBAI	YACGTR	2 cut(s)	3967, 4970
BstDSI	CCRYGG	2 cut(s)	3303, 4021
BstEII	GGTNACC	1 cut(s)	5227
BstFNI	CGCG	2 cut(s)	1020, 5610
BstH2I	RGCGCY	2 cut(s)	1851, 4580
BstHHI	GCGC	3 cut(s)	1715, 1850, 4579
BstNSI	RCATGY	6 cut(s)	1433, 1961, 2809, 2998, 4069, 5585
BstPI	GGTNACC	1 cut(s)	5227
BstSFI	CTRYAG	6 cut(s)	513, 1983, 3617, 3741, 5184, 5551
BstSLI	GKGCMC	1 cut(s)	500
BstSNI	TACGTA	1 cut(s)	3967
BstUI	CGCG	2 cut(s)	1020, 5610
BstV2I	GAAGAC	1 cut(s)	980
BstX2I	RGATCY	9 cut(s)	2869, 2943, 2959, 3034, 3115, 3274, 3325, 3613, 5012
BstXI	CCANNNNNNTGG	2 cut(s)	1052, 1616
BstYI	RGATCY	9 cut(s)	2869, 2943, 2959, 3034, 3115, 3274, 3325, 3613, 5012
BstZ17I	GTATAC	1 cut(s)	4407
Bsu15I	ATCGAT	3 cut(s)	1181, 3104, 5352
Bsu36I	CCTNAGG	2 cut(s)	885, 2109
BsuI	GTATCC	2 cut(s)	1072, 2491
BsuTUI	ATCGAT	3 cut(s)	1181, 3104, 5352
BtgI	CCRYGG	2 cut(s)	3303, 4021
BtrI	CACGTC	2 cut(s)	3869, 5177
BtsI	GCAGTG	2 cut(s)	144, 274
BtuMI	TCGCGA	1 cut(s)	1020
BveI	ACCTGC	1 cut(s)	4911
CaiI	CAGNNNCTG	2 cut(s)	1669, 5377
CciI	TCATGA	3 cut(s)	1900, 4629, 5038
CfoI	GCGC	3 cut(s)	1715, 1850, 4579
Cfr10I	RCCGGY	1 cut(s)	5150
ClaI	ATCGAT	3 cut(s)	1181, 3104, 5352
CpoI	CGGWCCG	1 cut(s)	5257
CseI	GACGC	2 cut(s)	2011, 3470
CsiI	ACCWGGT	1 cut(s)	2530
Csp6I	GTAC	7 cut(s)	2535, 2632, 3236, 3651, 3790, 3995, 5606
CspCI	CAANNNNNGTGG	2 cut(s)	5551, 5586
CspI	CGGWCCG	1 cut(s)	5257
CviQI	GTAC	7 cut(s)	2535, 2632, 3236, 3651, 3790, 3995, 5606
DraI	TTTAAA	1 cut(s)	5406
DraIII	CACNNNGTG	2 cut(s)	1219, 1694
DriI	GACNNNNNGTC	1 cut(s)	3872
EaeI	YGGCCR	2 cut(s)	144, 2164
Eam1105I	GACNNNNNGTC	1 cut(s)	3872
Eco105I	TACGTA	1 cut(s)	3967
Eco130I	CCWWGG	4 cut(s)	1593, 3269, 3421, 3442
Eco147I	AGGCCT	1 cut(s)	595
Eco24I	GRGCYC	1 cut(s)	4030
Eco31I	GGTCTC	1 cut(s)	1276
Eco32I	GATATC	1 cut(s)	4223
Eco47I	GGWCC	6 cut(s)	1408, 1417, 1485, 3132, 4540, 5257
Eco47III	AGCGCT	1 cut(s)	4578
Eco57I	CTGAAG	4 cut(s)	879, 887, 5313, 5445
Eco72I	CACGTG	1 cut(s)	4970
Eco81I	CCTNAGG	2 cut(s)	885, 2109
Eco88I	CYCGRG	3 cut(s)	606, 3697, 3757
Eco91I	GGTNACC	1 cut(s)	5227
EcoO109I	RGGNCCY	4 cut(s)	1417, 2747, 4540, 4774
EcoO65I	GGTNACC	1 cut(s)	5227
EcoRI	GAATTC	1 cut(s)	577
EcoRV	GATATC	1 cut(s)	4223
EcoT14I	CCWWGG	4 cut(s)	1593, 3269, 3421, 3442
EcoT22I	ATGCAT	2 cut(s)	4315, 5253
EcoT38I	GRGCYC	1 cut(s)	4030
ErhI	CCWWGG	4 cut(s)	1593, 3269, 3421, 3442
Esp3I	CGTCTC	1 cut(s)	4368
FalI	AAGNNNNNCTT	6 cut(s)	3759, 3791, 4245, 4277, 4763, 4795
FaqI	GGGAC	7 cut(s)	44, 653, 665, 2949, 3284, 3883, 4094
FauI	CCCGC	1 cut(s)	185
FauNDI	CATATG	2 cut(s)	3799, 5519
FbaI	TGATCA	2 cut(s)	199, 1806
FblI	GTMKAC	2 cut(s)	3015, 4406
FriOI	GRGCYC	1 cut(s)	4030
GlaI	GCGC	3 cut(s)	1714, 1849, 4578
GsaI	CCCAGC	2 cut(s)	972, 1590
GsuI	CTGGAG	5 cut(s)	3588, 3806, 4535, 4839, 4899
HaeII	RGCGCY	2 cut(s)	1851, 4580
HapII	CCGG	6 cut(s)	239, 1411, 3904, 4472, 5151, 5255
HgaI	GACGC	2 cut(s)	2011, 3470
HhaI	GCGC	3 cut(s)	1715, 1850, 4579
Hin1I	GRCGYC	1 cut(s)	2003
Hin6I	GCGC	3 cut(s)	1713, 1848, 4577
HinP1I	GCGC	3 cut(s)	1713, 1848, 4577
HincII	GTYRAC	5 cut(s)	1318, 1654, 2851, 4926, 4951
HindII	GTYRAC	5 cut(s)	1318, 1654, 2851, 4926, 4951
HindIII	AAGCTT	3 cut(s)	2155, 2705, 3561
HpaI	GTTAAC	3 cut(s)	1318, 1654, 4951
HpaII	CCGG	6 cut(s)	239, 1411, 3904, 4472, 5151, 5255
Hpy99I	CGWCG	1 cut(s)	4458
HpyCH4III	ACNGT	9 cut(s)	35, 215, 903, 2289, 2326, 2539, 3546, 4005, 5338
HpyCH4IV	ACGT	5 cut(s)	3868, 3966, 4969, 5176, 5597
HpySE526I	ACGT	5 cut(s)	3868, 3966, 4969, 5176, 5597
Hsp92I	GRCGYC	1 cut(s)	2003
HspAI	GCGC	3 cut(s)	1713, 1848, 4577
Ksp22I	TGATCA	2 cut(s)	199, 1806
KspAI	GTTAAC	3 cut(s)	1318, 1654, 4951
LguI	GCTCTTC	5 cut(s)	483, 855, 929, 1593, 3031
MabI	ACCWGGT	1 cut(s)	2530
MaeII	ACGT	5 cut(s)	3868, 3966, 4969, 5176, 5597
MbiI	CCGCTC	1 cut(s)	493
MfeI	CAATTG	2 cut(s)	908, 2211
MflI	RGATCY	9 cut(s)	2869, 2943, 2959, 3034, 3115, 3274, 3325, 3613, 5012
MhlI	GDGCHC	4 cut(s)	500, 4030, 4682, 5023
MlsI	TGGCCA	2 cut(s)	146, 2166
MluI	ACGCGT	1 cut(s)	5608
MluNI	TGGCCA	2 cut(s)	146, 2166
MlyI	GAGTC	4 cut(s)	3040, 3953, 4444, 5678
MmeI	TCCRAC	5 cut(s)	566, 2569, 3539, 4621, 4821
Mox20I	TGGCCA	2 cut(s)	146, 2166
Mph1103I	ATGCAT	2 cut(s)	4315, 5253
MroXI	GAANNNNTTC	2 cut(s)	879, 1014
MscI	TGGCCA	2 cut(s)	146, 2166
MslI	CAYNNNNRTG	4 cut(s)	1050, 1087, 1962, 2075
Msp20I	TGGCCA	2 cut(s)	146, 2166
MspA1I	CMGCKG	4 cut(s)	565, 3689, 4837, 5423
MspCI	CTTAAG	1 cut(s)	4085
MspI	CCGG	6 cut(s)	239, 1411, 3904, 4472, 5151, 5255
MunI	CAATTG	2 cut(s)	908, 2211
Mva1269I	GAATGC	5 cut(s)	2417, 2434, 4710, 5395, 5648
MvnI	CGCG	2 cut(s)	1020, 5610
NciI	CCSGG	2 cut(s)	3904, 4472
NdeI	CATATG	2 cut(s)	3799, 5519
NheI	GCTAGC	1 cut(s)	4072
NmeAIII	GCCGAG	2 cut(s)	5666, 5688
NruI	TCGCGA	1 cut(s)	1020
NsiI	ATGCAT	2 cut(s)	4315, 5253
NspI	RCATGY	6 cut(s)	1433, 1961, 2809, 2998, 4069, 5585
PaeI	GCATGC	1 cut(s)	4069
PagI	TCATGA	3 cut(s)	1900, 4629, 5038
PceI	AGGCCT	1 cut(s)	595
PciI	ACATGT	1 cut(s)	2994
PciSI	GCTCTTC	5 cut(s)	483, 855, 929, 1593, 3031
PctI	GAATGC	5 cut(s)	2417, 2434, 4710, 5395, 5648
PdmI	GAANNNNTTC	2 cut(s)	879, 1014
PflMI	CCANNNNNTGG	7 cut(s)	1482, 2788, 2879, 3270, 3521, 4007, 4817
PfoI	TCCNGGA	1 cut(s)	2878
PleI	GAGTC	4 cut(s)	3040, 3952, 4444, 5678
PmaCI	CACGTG	1 cut(s)	4970
PmlI	CACGTG	1 cut(s)	4970
PpsI	GAGTC	4 cut(s)	3040, 3952, 4444, 5678
Ppu21I	YACGTR	2 cut(s)	3967, 4970
PpuMI	RGGWCCY	2 cut(s)	1417, 4540
PscI	ACATGT	1 cut(s)	2994
PshBI	ATTAAT	1 cut(s)	1185
PsiI	TTATAA	1 cut(s)	1169
Psp5II	RGGWCCY	2 cut(s)	1417, 4540
PspCI	CACGTG	1 cut(s)	4970
PspEI	GGTNACC	1 cut(s)	5227
PspFI	CCCAGC	2 cut(s)	968, 1586
PspPPI	RGGWCCY	2 cut(s)	1417, 4540
PstI	CTGCAG	3 cut(s)	3621, 3745, 5188
PstNI	CAGNNNCTG	2 cut(s)	1669, 5377
PsuI	RGATCY	9 cut(s)	2869, 2943, 2959, 3034, 3115, 3274, 3325, 3613, 5012
PvuII	CAGCTG	3 cut(s)	565, 3689, 5423
RruI	TCGCGA	1 cut(s)	1020
RsaI	GTAC	7 cut(s)	2536, 2633, 3237, 3652, 3791, 3996, 5607
RsaNI	GTAC	7 cut(s)	2535, 2632, 3236, 3651, 3790, 3995, 5606
RseI	CAYNNNNRTG	4 cut(s)	1050, 1087, 1962, 2075
Rsr2I	CGGWCCG	1 cut(s)	5257
RsrII	CGGWCCG	1 cut(s)	5257
SapI	GCTCTTC	5 cut(s)	483, 855, 929, 1593, 3031
SchI	GAGTC	4 cut(s)	3040, 3953, 4444, 5678
SduI	GDGCHC	4 cut(s)	500, 4030, 4682, 5023
SexAI	ACCWGGT	1 cut(s)	2530
SfcI	CTRYAG	6 cut(s)	513, 1983, 3617, 3741, 5184, 5551
SinI	GGWCC	6 cut(s)	1408, 1417, 1485, 3132, 4540, 5257
SmiMI	CAYNNNNRTG	4 cut(s)	1050, 1087, 1962, 2075
SmlI	CTYRAG	4 cut(s)	2036, 3048, 4085, 4525
SmoI	CTYRAG	4 cut(s)	2036, 3048, 4085, 4525
SnaBI	TACGTA	1 cut(s)	3967
SpeI	ACTAGT	1 cut(s)	2255
SphI	GCATGC	1 cut(s)	4069
SseBI	AGGCCT	1 cut(s)	595
SspI	AATATT	3 cut(s)	2696, 2926, 4756
StuI	AGGCCT	1 cut(s)	595
StyI	CCWWGG	4 cut(s)	1593, 3269, 3421, 3442
TaaI	ACNGT	9 cut(s)	35, 215, 903, 2289, 2326, 2539, 3546, 4005, 5338
TaiI	ACGT	5 cut(s)	3871, 3969, 4972, 5179, 5600
TaqII	GACCGA	1 cut(s)	4943
TatI	WGTACW	3 cut(s)	3235, 3789, 3994
TauI	GCSGC	3 cut(s)	17, 395, 4376
TspGWI	ACGGA	8 cut(s)	1378, 2051, 2440, 2470, 4010, 4350, 4471, 5249
Van91I	CCANNNNNTGG	7 cut(s)	1482, 2788, 2879, 3270, 3521, 4007, 4817
Vha464I	CTTAAG	1 cut(s)	4085
VpaK11BI	GGWCC	6 cut(s)	1408, 1417, 1485, 3132, 4540, 5257
VspI	ATTAAT	1 cut(s)	1185
XapI	RAATTY	9 cut(s)	121, 346, 577, 1137, 2219, 2578, 2651, 4760, 4960
XbaI	TCTAGA	1 cut(s)	1547
XceI	RCATGY	6 cut(s)	1433, 1961, 2809, 2998, 4069, 5585
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2326, 3518
XmaJI	CCTAGG	1 cut(s)	3442
XmiI	GTMKAC	2 cut(s)	3015, 4406
XmnI	GAANNNNTTC	2 cut(s)	879, 1014
Zsp2I	ATGCAT	2 cut(s)	4315, 5253

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.