Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Forward (+)

14341553 .. 14350247

Gene Structure 8695 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027722

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5865 bp

ATGGATACAGAGGCAGCTAGGGTTACAGCGGACAGTGGTGGCAATGGGGGAGAGGCGGTTCGGGCTTCTTACTGGCTGGATGCTTGTGAGGACATTGGGGATTTTGTGGACTTTTCCGAAAATTCGGGTGCTACGGCTGTGGTGGCGAGTGGGAATGGTTGCAATCAGGAGGAGGTGGGTGATTTTTTTGGCGGGATTGATCGCATTCTTGACAGTATCAAAAGTGGAGCTGGATTGCCGGATGGGAAGAGGGATGGGGTAGTGAGCAGTGGCAATGTGGAGGAGGTTGGGGTGACTGGCGTTTCCAATGGACTGAGTGGTGAGAATGGTCAGAGTGGGGGTCGAAATTCTGAGGGTGCTAATGGAGATGGGAGAGTGGCGGTGGTGGCGGCTTCTGCTGCTAAGCGTAATCACAATGGCTTGAGCAAGTATGAGAGTAATGGGAGGTGGAATCGGGGTGTGAGGGAAAGAGATGTGAATGGTGAAGAGCGGTGCCCCAAGAGGGTTGCGGTAGATAATGGTAGGAATGAGAGGTATAGTTCAGGCAGAGTGCAATATCAGCTGAGGGAGAGGAATTCTGGTCGGAAGAGGCCTCGTGATCCTTGGGATGACATTGATAGGAGGGATAGGGACAGAGATAGGGACAGGGATAGAGATCGGACTAGGAGAAGAGAGAGTTATGGTAGTAATAGGAGGGATAGTAGAGATTGGGAAGCAAAGGGCTATTGGGAGAGGGATAAATTGGGGTCAAATGAGCTTGTTTTTAGATTAGGAGCTTATGAACCTCATCAGAAGAAAGAGGAGAAAGTCGCTAATGACAAAATCAATGAAAAGGATGTGAAGAAATCTGAAGAGCTGAAGAAAGAAAAGATTCCTGAGGAACGAGCCAGACAGTATCAATTGGATGTTCTTGAACAGGCAAAGAAGAGCAATACGATAGCTTTTCTTGAAACTGGGGCTGGGAAGACACTCATTGCCATTCTCCTCATGCAAAGTGTCTGCAATGATTTGCAAAAGAAGAATAAAAAGATGCTTTCTGTGTTTCTAGTTCCTAAAGTTCCGCTTGTTTATCAGCAAGCAGAAGTTATTCGTGAGCGAACTGGTTTCCAAGTGGGCCATTATTGTGGTGAGATGGGTCAAGATTTTTGGGATACACGAAGATGGCAGCGTGAGTTTGATACTAAACAGGTTCTAGTAATGACAGCTCAAATTCTATTGAACATTCTAAGACACAGCATTATAAAGATGGAATCGATTAATCTATTGATTCTGGATGAGTGCCATCACGCAGTGAAGAAACATCCATACTCTTTAGTGATGTCCGAGTTCTATCATACAACCCCAAAAGAGAAGAGACCATCTGTATTTGGAATGACTGCTTCTCCTGTTAACTTAAAAGGTGTTTCCAATCAACTAGATTGTGCAATAAAGATTCGTAATCTTGAAAGTAAACTGGATTCCGTTGTTTGCACCATAAAGGACCGGAAGGACCTGGAAAAGCATGTTCCGATGCCCTCAGAAATTGTTGTGGAGTATGACAAAGCAGCCAGCTTATGGTCCTTTCACGAACAGCTAAAACAAATGGAAGTGGAAGTTGAAGAAGCTGCAAAGTCTAGCTCTAGAAGAAGTAAATGGCAGTTTATGGGAGCTAGAGATGCTGGGGCCAAGGAAGAGCTACGCCAAGTGTATGGTGTTTCAGAAAGAACAGAAAGTGATGGGGCTGTTAACCTAATTCAGAAGCTGAGGGCTATCAATTATGCACTTGGTGAACTGGGTCAGTGGTGTGCTTACAAGGTGGCACAATCATTCTTGACAGCTTTACAAAATGATGAGAGGGCAAACTACCAGCTTGATGTCAAGTTTCAAGAAACTTACCTGATCAGAGTTGTATCTCTTTTACAATGTCATCTGTCAGAGGGTGCTGCTTCTGACAAGGAAACAAAACTGCCAGAATCAGAGAGTGGCGTTTCTCATTACGAAATTGAGGAAGGAGAGCTACCTGATAGTCATGTTGTTTCTGGTGGGGAGCATGTAGATGTGATAATAGGAGCTGCTGTAGCTGATGGGAAGGTGACGCCAAAAGTGCAGTCACTAATTAAAGTTCTTCTCAAGTATCAGCATACAGAAGATTTCCGTGCAATCATCTTTGTGGAGCGAGTTGTCTCCGCTTTAGTTCTTCCTAAGGTCTTTGCTGAGCTTCCATCTCTAGGTTTTATCGAGTGTGCAAGCTTGATTGGCCACAATAATAGTCAGGAAATGCGAAGTAGCCAAATGCAGGACACAATTGCCAAATTCAAGGATGGTCGTGTGACATTGTTAGTTGCAACTAGTGTTGCTGAGGAAGGACTTGATATTCGACAGTGCAATGTTGTTATTCGCTTCGACCTTGCAAAAACTGTTTTGGCTTATATTCAGTCTAGGGGCCGTGCTAGAAAACCTGGGTCAGATTATATCTTGATGGTGGAAAGGGCAAATCTGTCACATGAAGCATTCTTACGGAATGCTAGAAATAGCGAGGAGACTTTACGGAAAGAAGCAATAGAGAGGACCGACCTGAGTGATTTGAAGGATACTTCAAGGTTAATTTCAGTGGAAACAGCACCAGGTACAGTGTATCAGGTGGAATCAACTGGTGCTTTGGTGAGCTTAAATTCTGCTGTTGGACTAATCCATTTCTACTGCTCTCAGCTTCCGAGTGACAGGTACTCAATACTTCATCCTGAATTTGTTATGGTGAGGCATGAGAAGCAAGAAGGCCCGACTGAATATTCATGCAAGCTTCAACTCCCTTGTAATGCACCATTTGAGACACTTGAAGGCCCTGTGTGCAGTTCAATGCACCTTGCGCAACAGGCTGTTTGTTTGGCTGCTTGTAAGAAACTCCATGAGATGGGAGCATTTACTGACATGCTCTTGCCCGACAGGGGAGTTGGGGAAGAAAAAGAAAAGGTTGACAAGAATGACGAAGGAGATCCACTTCCTGGAACTGCTAGGCACAGAGAGTTCTATCCCGAAGGTGTAGCTAATATTCTCCAGGGAGAATGGATCTTAGCTGGAAGAGATCTTGGCAATGAGGCCAAATTGATTCATGTTTACATGTATTCTGTGAAATGTGTAGACATTGGCTCTTCAAAGGATCCTTTCTTGACTCAAGTTTCAGATTTTGCAATTCTATTAGGCAATGAGCTGGATGCAGAGGTGTTATCGATGTCGATGGATCTATTTGTTGCTCGGACCATGACTACAAAGGCTTCCCTTGTCTTTAGGGGTTCAATTCGTATTACTGAAAGTCAGTTGGCATCCCTTAAAAGTTTCCATGTAAGATTGATGAGTATTGTGCTGGATGTGGATGTTGAACCTTCCACCACTCCTTGGGATCCTGCAAAGGCTTATTTATTTGTCCCCGTGGTTAGCAATAAATGTGCAGATCCTATGAAAGAAATAGATTGGGATCTGGTTGAAAATATAATTGGTGCTGATGCGTGGAACAATCCCCTTCAAAGAGCTAGGCCCGATGTTTACCTTGGCACAAATGAGCGAACCCTAGGAGGTGACAGAAGGGAGTATGGTTTTGGTAAACTGCGTCATGGCATGGCTTTTGGGCAAAAATCCCATCCAACCTATGGTATCAGAGGAGCTGTGGCACAGTTTGATGTTGTTAAAGCTTGTGGACTGATCCCTGATCGGGATGCCTTTGAAATGCAAAAGGATATGGATCTGCCGCAACGCAAGTTGATGATGGCAGATAGTTGTACCAATGTTGAAGATTTGGTAGGGAAAATTGTGACAGCTGCTCACTCGGGGAAGAGATTTTATGTTGATTCAATATGCTATGATATGACTGCAGAAAACTCTTTCCCGAGGAAAGAAGGTTATCTTGGCCCTCTGGAGTACAGCTCATATGCTGATTATTACAAGCAAAAGTATGGAGTTCAGTTGATGTATAAGAAACAACCTCTAATAAAAGGACGTGGTGTCTCATATTGTAAGAATCTCCTGTCCCCCCGGTTTGACCACATGGAAGAACTTGAAGGTGAATCAGAAGAGTCTCTGGACAAAACATACTACGTATTTCTCCCTCCTGAGCTTTGTTTAGTACACCCACTGTCTGGCTCACTTGTCCGTGGAGCCCAAAGATTACCCTCAATAATGAGAAGGGTTGAAAGCATGCTGCTAGCGGTTGAACTTAAGGAGATAATAAATTATCCTGTCCCTGCTTTTAAGATTTTGGAAGCATTGACTGCTGCTTCATGCCAGGAGACATTTTGCTATGAAAGAGCTGAGCTTCTGGGTGATGCCTACCTCAAATGGGTTGTTAGTCGATTTCTTTTTCTTAAATACCCTCAGAAACACGAAGGTCAGCTTACTAGGATGAGACAACAAATGGTCAGTAACATGGTTTTGTATCATTATGCATTGAAAAGAGGACTTCAATCATATATCCAAGCAGATCGCTTTGCTCCGTCTCGATGGGCGGCTCCTGGGGTGTTGCCTGTCTTTGATGAATATACAAAGGATGAAGAATCATCTTTGTTTGACCAAGAGGATGTGACTCGATGGAAAACAGATGACCCGGTTAATGAATATGAGGACGATGAATTGGAGGATGGCGAGCTGGAGAGTGACTTGAGTTCTTATAGAGTCCTGTCAAGCAAGACACTTGCAGATGTTGTTGAAGCGCTTATTGGGGTTTATTATGTTGAAGGTGGGAAAAAGGCTGCTAACCATCTCATGAAATGGGTTGGAATTGATGTAGAGTTTAATGCTGATGAGATAGAGAGCACAACAAGGCCATCTAATGTTCCAGATAGCATTCTTAGGAGCATCGACTTTGATGCATTAGAGGGTGCTTTGAATATTAAATTTAGAGACAAGGGCCTCTTGGTGGAAGCCATTAGTCATGCTTCCCGACCATCTTCTGGAGTAGCCTGCTATCAGCGGTTGGAGTTTGTGGGTGATGCTGTCTTAGATCATCTCATCACAAAGCACTTGTTCTTCACATACACAAATCTGCCTCCAGGCAGGTTGACCGATCTTAGAGCTGCTGCTGTTAACAATGAAAATTTTGCACGTGTTGCTGTAAAACATAATCTCCATCTACACCTTCGACATGGATCCAGTGCTCTTGAAAGACAGATTCATGACTTTGTGAAGGAAGCTGCAAATGAATTAACAAAGCCTGGGTTCAACTCATTTGGTTTGGGAGATTGTAAAGCTCCCAAAGTTCTTGGTGACATTATTGAATCTATTGCCGGTGCCATTTTTCTCGATAGCGGACGTGATACTGCAGTTGTTTGGAAGGTGTTTCAACCTCTGCTTCAACCAATGGTCACCCCAGAGACACTACCAATGCATCCGGTCCGTGAGCTGCAAGAAAGATGCCAGCAACAGGCTGAAGGCTTGGAATACAAAGCCAGTCGAAGTGGCAATTTGGCTACTGTTGAAGTCTTGATCGATGGTGTCAAGGTAGGAATTGCTCAGAATCCACAAAAGAAGATGGCACAGAAACTGGCTGCCAGGAATGCTCTTGCTGCTTTAAAAGATAAGGACACCGCTGAAGCAAAGGAGAGGCAGGAGAGGGAGGAGGAAGATAATGGGAAGAAGAAGAAGAATGGTAGCCAAACATTTACCAGGCAAACCTTGAATGACATATGCTTGCGTAAAAACTGGCCTATGCCATTCTACAGGTGTGTCAATGAGGGTGGCCCTGCTCATGCAAAGAAGTTTACGTTTGCTGTACGCGTGAATACCACTGACAGGGGATGGATAGACGAATGCGTAGGGGAGCCTATGCCGAGTGTCAAGAAAGCCAAAGACTCGGCAGCAGTTCTTCTATTGGAATTATTGAATAAGTTATACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1955

Amino Acids

219.29

Weight (kDa)

5.99

Isoelectric Point (pI)

42.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	297 - 462	1.7e-14	Type III restriction enzyme, res subunit
DEAD	PF00270	298 - 462	9.1e-16	DEAD/DEAH box helicase
Helicase_C	PF00271	694 - 811	1.6e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	886 - 974	4.4e-22	Dicer dimerisation domain
PAZ	PF02170	1253 - 1387	3.8e-18	PAZ domain
Ribonucleas_3_3	PF14622	1414 - 1483	1.5e-07	Ribonuclease-III-like
Ribonuclease_3	PF00636	1425 - 1570	1.4e-20	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1624 - 1764	1.5e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1646 - 1759	1.7e-22	Ribonuclease III domain
dsrm	PF00035	1786 - 1846	1e-05	Double-stranded RNA binding motif
DND1_DSRM	PF14709	1876 - 1949	1.7e-15	double strand RNA binding domain from DEAD END PROTEIN 1
dsrm	PF00035	1879 - 1949	2.4e-07	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1241
Acc16I	TGCGCA	1 cut(s)	2847
Acc36I	ACCTGC	1 cut(s)	5013
AccB1I	GGYRCC	2 cut(s)	492, 5255
AccB7I	CCANNNNNTGG	7 cut(s)	1554, 2890, 2981, 3372, 3623, 4109, 4919
AccBSI	CCGCTC	1 cut(s)	490
AccI	GTMKAC	1 cut(s)	3117
AccII	CGCG	1 cut(s)	5742
AcoI	YGGCCR	1 cut(s)	2236
AcsI	RAATTY	9 cut(s)	121, 346, 574, 1209, 2291, 2650, 2723, 4862, 5062
AcuI	CTGAAG	4 cut(s)	870, 878, 5415, 5577
AcvI	CACGTG	1 cut(s)	5072
AcyI	GRCGYC	1 cut(s)	2075
AdeI	CACNNNGTG	2 cut(s)	1291, 1766
AfaI	GTAC	6 cut(s)	2608, 2705, 3754, 3893, 4098, 5739
AfeI	AGCGCT	1 cut(s)	4680
AflII	CTTAAG	1 cut(s)	4187
AflIII	ACRYGT	3 cut(s)	3096, 5071, 5740
AhdI	GACNNNNNGTC	1 cut(s)	3974
AhlI	ACTAGT	1 cut(s)	2327
AjiI	CACGTC	2 cut(s)	3971, 5279
AjuI	GAANNNNNNNTTGG	6 cut(s)	3453, 3485, 3861, 3893, 4668, 4700
AloI	GAACNNNNNNTCC	2 cut(s)	42, 74
Alw21I	GWGCWC	2 cut(s)	4784, 5125
AlwNI	CAGNNNCTG	2 cut(s)	1741, 5509
Ama87I	CYCGRG	2 cut(s)	3799, 3859
Aor51HI	AGCGCT	1 cut(s)	4680
ApoI	RAATTY	9 cut(s)	121, 346, 574, 1209, 2291, 2650, 2723, 4862, 5062
ArsI	GACNNNNNNTTYG	4 cut(s)	2072, 2104, 2337, 2369
AseI	ATTAAT	1 cut(s)	1257
Asp700I	GAANNNNTTC	2 cut(s)	870, 1086
AspA2I	CCTAGG	1 cut(s)	3544
AspLEI	GCGC	2 cut(s)	2848, 4681
AsuC2I	CCSGG	2 cut(s)	4006, 4574
AsuNHI	GCTAGC	1 cut(s)	4174
AvaI	CYCGRG	2 cut(s)	3799, 3859
AvaII	GGWCC	6 cut(s)	1480, 1489, 1557, 2547, 3234, 5359
AvrII	CCTAGG	1 cut(s)	3544
AxyI	CCTNAGG	2 cut(s)	876, 2181
BaeGI	GKGCMC	1 cut(s)	497
BaeI	ACNNNNGTAYC	2 cut(s)	5274, 5307
BalI	TGGCCA	1 cut(s)	2238
BamHI	GGATCC	3 cut(s)	3136, 3376, 5114
BanI	GGYRCC	2 cut(s)	492, 5255
BanII	GRGCYC	1 cut(s)	4132
BarI	GAAGNNNNNNTAC	4 cut(s)	1980, 2012, 2478, 2510
BauI	CACGAG	1 cut(s)	594
BbrPI	CACGTG	1 cut(s)	5072
BbsI	GAAGAC	1 cut(s)	971
Bbv12I	GWGCWC	2 cut(s)	4784, 5125
BbvCI	CCTCAGC	3 cut(s)	563, 1742, 2337
BceAI	ACGGC	2 cut(s)	150, 2409
BcgI	CGANNNNNNTGC	2 cut(s)	4817, 4851
BciVI	GTATCC	2 cut(s)	1144, 2563
BclI	TGATCA	1 cut(s)	1878
BcnI	CCSGG	2 cut(s)	4006, 4574
BcuI	ACTAGT	1 cut(s)	2327
BfmI	CTRYAG	4 cut(s)	2055, 3843, 5286, 5683
BfoI	RGCGCY	1 cut(s)	4682
BfrI	CTTAAG	1 cut(s)	4187
BfuAI	ACCTGC	1 cut(s)	5013
BfuI	GTATCC	2 cut(s)	1144, 2563
BglII	AGATCT	1 cut(s)	3061
BlnI	CCTAGG	1 cut(s)	3544
BlpI	GCTNAGC	3 cut(s)	402, 2193, 4281
Bme18I	GGWCC	6 cut(s)	1480, 1489, 1557, 2547, 3234, 5359
BmeRI	GACNNNNNGTC	1 cut(s)	3974
BmeT110I	CYCGRG	2 cut(s)	3799, 3859
BmgBI	CACGTC	2 cut(s)	3971, 5279
BmrI	ACTGGG	2 cut(s)	963, 1781
BmtI	GCTAGC	1 cut(s)	4178
BmuI	ACTGGG	2 cut(s)	963, 1781
BpiI	GAAGAC	1 cut(s)	971
BplI	GAGNNNNNCTC	2 cut(s)	3881, 3913
BpmI	CTGGAG	5 cut(s)	3017, 3908, 4637, 4941, 5001
Bpu10I	CCTNAGC	4 cut(s)	563, 1742, 2337, 4083
Bpu1102I	GCTNAGC	3 cut(s)	402, 2193, 4281
BpuEI	CTTGAG	4 cut(s)	442, 2093, 3135, 4648
BpuMI	CCSGG	2 cut(s)	4006, 4574
Bsa29I	ATCGAT	3 cut(s)	1253, 3206, 5454
BsaAI	YACGTR	2 cut(s)	4069, 5072
BsaBI	GATNNNNATC	3 cut(s)	654, 3450, 3714
BsaHI	GRCGYC	1 cut(s)	2075
BsaI	GGTCTC	1 cut(s)	1348
BsaWI	WCCGGW	2 cut(s)	1482, 5356
Bse118I	RCCGGY	1 cut(s)	5252
Bse21I	CCTNAGG	2 cut(s)	876, 2181
Bse3DI	GCAATG	7 cut(s)	49, 280, 972, 1009, 2371, 3076, 3187
Bse8I	GATNNNNATC	3 cut(s)	654, 3450, 3714
BseCI	ATCGAT	3 cut(s)	1253, 3206, 5454
BseJI	GATNNNNATC	3 cut(s)	654, 3450, 3714
BseMI	GCAATG	7 cut(s)	49, 280, 972, 1009, 2371, 3076, 3187
BseRI	GAGGAG	7 cut(s)	185, 296, 815, 974, 2531, 3648, 5597
BseSI	GKGCMC	1 cut(s)	497
BseYI	CCCAGC	2 cut(s)	959, 1658
BsgI	GTGCAG	3 cut(s)	2105, 2848, 3444
Bsh1236I	CGCG	1 cut(s)	5742
BshNI	GGYRCC	2 cut(s)	492, 5255
BshVI	ATCGAT	3 cut(s)	1253, 3206, 5454
BsiHKAI	GWGCWC	2 cut(s)	4784, 5125
BsiHKCI	CYCGRG	2 cut(s)	3799, 3859
BsiSI	CCGG	6 cut(s)	239, 1483, 4006, 4574, 5253, 5357
BslFI	GGGAC	5 cut(s)	644, 656, 3386, 3985, 4196
BsmBI	CGTCTC	1 cut(s)	4470
BsmFI	GGGAC	5 cut(s)	644, 656, 3386, 3985, 4196
BsmI	GAATGC	6 cut(s)	204, 2489, 2506, 4812, 5527, 5780
Bso31I	GGTCTC	1 cut(s)	1348
BsoBI	CYCGRG	2 cut(s)	3799, 3859
Bsp1286I	GDGCHC	4 cut(s)	497, 4132, 4784, 5125
Bsp1720I	GCTNAGC	3 cut(s)	402, 2193, 4281
BspDI	ATCGAT	3 cut(s)	1253, 3206, 5454
BspFNI	CGCG	1 cut(s)	5742
BspHI	TCATGA	2 cut(s)	4731, 5140
BspMAI	CTGCAG	2 cut(s)	3847, 5290
BspMI	ACCTGC	1 cut(s)	5013
BspOI	GCTAGC	1 cut(s)	4178
BspQI	GCTCTTC	5 cut(s)	480, 846, 920, 1665, 3133
BspT107I	GGYRCC	2 cut(s)	492, 5255
BspTI	CTTAAG	1 cut(s)	4187
BspTNI	GGTCTC	1 cut(s)	1348
BsrBI	CCGCTC	1 cut(s)	490
BsrDI	GCAATG	7 cut(s)	49, 280, 972, 1009, 2371, 3076, 3187
BsrFI	RCCGGY	1 cut(s)	5252
BssAI	RCCGGY	1 cut(s)	5252
BssNI	GRCGYC	1 cut(s)	2075
BssSI	CACGAG	1 cut(s)	594
BssT1I	CCWWGG	5 cut(s)	602, 1665, 3371, 3523, 3544
Bst2BI	CACGAG	1 cut(s)	594
Bst4CI	ACNGT	9 cut(s)	35, 215, 894, 2361, 2398, 2611, 3648, 4107, 5440
BstACI	GRCGYC	1 cut(s)	2075
BstAFI	CTTAAG	1 cut(s)	4187
BstAPI	GCANNNNNTGC	2 cut(s)	4241, 5075
BstBAI	YACGTR	2 cut(s)	4069, 5072
BstDSI	CCRYGG	2 cut(s)	3405, 4123
BstEII	GGTNACC	1 cut(s)	5329
BstFNI	CGCG	1 cut(s)	5742
BstH2I	RGCGCY	1 cut(s)	4682
BstHHI	GCGC	2 cut(s)	2848, 4681
BstNSI	RCATGY	5 cut(s)	1505, 2033, 2911, 3100, 4171
BstPI	GGTNACC	1 cut(s)	5329
BstSFI	CTRYAG	4 cut(s)	2055, 3843, 5286, 5683
BstSLI	GKGCMC	1 cut(s)	497
BstSNI	TACGTA	1 cut(s)	4069
BstUI	CGCG	1 cut(s)	5742
BstV2I	GAAGAC	1 cut(s)	971
BstXI	CCANNNNNNTGG	2 cut(s)	1124, 1688
Bsu15I	ATCGAT	3 cut(s)	1253, 3206, 5454
Bsu36I	CCTNAGG	2 cut(s)	876, 2181
BsuI	GTATCC	2 cut(s)	1144, 2563
BsuTUI	ATCGAT	3 cut(s)	1253, 3206, 5454
BtgI	CCRYGG	2 cut(s)	3405, 4123
BtrI	CACGTC	2 cut(s)	3971, 5279
BtsI	GCAGTG	2 cut(s)	274, 1296
BveI	ACCTGC	1 cut(s)	5013
CaiI	CAGNNNCTG	2 cut(s)	1741, 5509
CciI	TCATGA	2 cut(s)	4731, 5140
CfoI	GCGC	2 cut(s)	2848, 4681
Cfr10I	RCCGGY	1 cut(s)	5252
ClaI	ATCGAT	3 cut(s)	1253, 3206, 5454
CpoI	CGGWCCG	1 cut(s)	5359
CseI	GACGC	2 cut(s)	2083, 3572
CsiI	ACCWGGT	1 cut(s)	2602
Csp6I	GTAC	6 cut(s)	2607, 2704, 3753, 3892, 4097, 5738
CspCI	CAANNNNNGTGG	2 cut(s)	5683, 5718
CspI	CGGWCCG	1 cut(s)	5359
CviQI	GTAC	6 cut(s)	2607, 2704, 3753, 3892, 4097, 5738
DraI	TTTAAA	1 cut(s)	5538
DraIII	CACNNNGTG	2 cut(s)	1291, 1766
DriI	GACNNNNNGTC	1 cut(s)	3974
EaeI	YGGCCR	1 cut(s)	2236
Eam1105I	GACNNNNNGTC	1 cut(s)	3974
Eco105I	TACGTA	1 cut(s)	4069
Eco130I	CCWWGG	5 cut(s)	602, 1665, 3371, 3523, 3544
Eco147I	AGGCCT	1 cut(s)	592
Eco24I	GRGCYC	1 cut(s)	4132
Eco31I	GGTCTC	1 cut(s)	1348
Eco47I	GGWCC	6 cut(s)	1480, 1489, 1557, 2547, 3234, 5359
Eco47III	AGCGCT	1 cut(s)	4680
Eco57I	CTGAAG	4 cut(s)	870, 878, 5415, 5577
Eco72I	CACGTG	1 cut(s)	5072
Eco81I	CCTNAGG	2 cut(s)	876, 2181
Eco88I	CYCGRG	2 cut(s)	3799, 3859
Eco91I	GGTNACC	1 cut(s)	5329
EcoO109I	RGGNCCY	3 cut(s)	1489, 2819, 4876
EcoO65I	GGTNACC	1 cut(s)	5329
EcoRI	GAATTC	1 cut(s)	574
EcoT14I	CCWWGG	5 cut(s)	602, 1665, 3371, 3523, 3544
EcoT22I	ATGCAT	3 cut(s)	4417, 4840, 5355
EcoT38I	GRGCYC	1 cut(s)	4132
ErhI	CCWWGG	5 cut(s)	602, 1665, 3371, 3523, 3544
Esp3I	CGTCTC	1 cut(s)	4470
FalI	AAGNNNNNCTT	8 cut(s)	1047, 1079, 3861, 3893, 4347, 4379, 4865, 4897
FaqI	GGGAC	5 cut(s)	644, 656, 3386, 3985, 4196
FauI	CCCGC	1 cut(s)	185
FauNDI	CATATG	2 cut(s)	3901, 5651
FbaI	TGATCA	1 cut(s)	1878
FblI	GTMKAC	1 cut(s)	3117
FriOI	GRGCYC	1 cut(s)	4132
FspI	TGCGCA	1 cut(s)	2847
GlaI	GCGC	2 cut(s)	2847, 4680
GsaI	CCCAGC	2 cut(s)	963, 1662
GsuI	CTGGAG	5 cut(s)	3017, 3908, 4637, 4941, 5001
HaeII	RGCGCY	1 cut(s)	4682
HapII	CCGG	6 cut(s)	239, 1483, 4006, 4574, 5253, 5357
HgaI	GACGC	2 cut(s)	2083, 3572
HhaI	GCGC	2 cut(s)	2848, 4681
Hin1I	GRCGYC	1 cut(s)	2075
Hin6I	GCGC	2 cut(s)	2846, 4679
HinP1I	GCGC	2 cut(s)	2846, 4679
HincII	GTYRAC	5 cut(s)	1390, 1726, 2953, 5028, 5053
HindII	GTYRAC	5 cut(s)	1390, 1726, 2953, 5028, 5053
HindIII	AAGCTT	3 cut(s)	2227, 2777, 3663
HpaI	GTTAAC	3 cut(s)	1390, 1726, 5053
HpaII	CCGG	6 cut(s)	239, 1483, 4006, 4574, 5253, 5357
HpyCH4III	ACNGT	9 cut(s)	35, 215, 894, 2361, 2398, 2611, 3648, 4107, 5440
HpyCH4IV	ACGT	5 cut(s)	3970, 4068, 5071, 5278, 5729
HpySE526I	ACGT	5 cut(s)	3970, 4068, 5071, 5278, 5729
Hsp92I	GRCGYC	1 cut(s)	2075
HspAI	GCGC	2 cut(s)	2846, 4679
Ksp22I	TGATCA	1 cut(s)	1878
KspAI	GTTAAC	3 cut(s)	1390, 1726, 5053
LguI	GCTCTTC	5 cut(s)	480, 846, 920, 1665, 3133
MabI	ACCWGGT	1 cut(s)	2602
MaeII	ACGT	5 cut(s)	3970, 4068, 5071, 5278, 5729
MbiI	CCGCTC	1 cut(s)	490
MfeI	CAATTG	2 cut(s)	899, 2283
MhlI	GDGCHC	4 cut(s)	497, 4132, 4784, 5125
MlsI	TGGCCA	1 cut(s)	2238
MluI	ACGCGT	1 cut(s)	5740
MluNI	TGGCCA	1 cut(s)	2238
MlyI	GAGTC	5 cut(s)	3142, 4055, 4546, 4650, 5810
MmeI	TCCRAC	5 cut(s)	563, 2641, 3641, 4723, 4923
Mox20I	TGGCCA	1 cut(s)	2238
Mph1103I	ATGCAT	3 cut(s)	4417, 4840, 5355
MroXI	GAANNNNTTC	2 cut(s)	870, 1086
MscI	TGGCCA	1 cut(s)	2238
MslI	CAYNNNNRTG	4 cut(s)	1122, 1159, 2034, 2147
Msp20I	TGGCCA	1 cut(s)	2238
MspA1I	CMGCKG	5 cut(s)	29, 562, 3791, 4939, 5555
MspCI	CTTAAG	1 cut(s)	4187
MspI	CCGG	6 cut(s)	239, 1483, 4006, 4574, 5253, 5357
MunI	CAATTG	2 cut(s)	899, 2283
Mva1269I	GAATGC	6 cut(s)	204, 2489, 2506, 4812, 5527, 5780
MvnI	CGCG	1 cut(s)	5742
NciI	CCSGG	2 cut(s)	4006, 4574
NdeI	CATATG	2 cut(s)	3901, 5651
NheI	GCTAGC	1 cut(s)	4174
NmeAIII	GCCGAG	2 cut(s)	5798, 5820
NsbI	TGCGCA	1 cut(s)	2847
NsiI	ATGCAT	3 cut(s)	4417, 4840, 5355
NspI	RCATGY	5 cut(s)	1505, 2033, 2911, 3100, 4171
PaeI	GCATGC	1 cut(s)	4171
PagI	TCATGA	2 cut(s)	4731, 5140
PceI	AGGCCT	1 cut(s)	592
PciI	ACATGT	1 cut(s)	3096
PciSI	GCTCTTC	5 cut(s)	480, 846, 920, 1665, 3133
PctI	GAATGC	6 cut(s)	204, 2489, 2506, 4812, 5527, 5780
PdmI	GAANNNNTTC	2 cut(s)	870, 1086
PflMI	CCANNNNNTGG	7 cut(s)	1554, 2890, 2981, 3372, 3623, 4109, 4919
PfoI	TCCNGGA	1 cut(s)	2980
PleI	GAGTC	5 cut(s)	3142, 4054, 4546, 4649, 5810
PmaCI	CACGTG	1 cut(s)	5072
PmlI	CACGTG	1 cut(s)	5072
PpsI	GAGTC	5 cut(s)	3142, 4054, 4546, 4649, 5810
Ppu21I	YACGTR	2 cut(s)	4069, 5072
PpuMI	RGGWCCY	1 cut(s)	1489
PscI	ACATGT	1 cut(s)	3096
PshBI	ATTAAT	1 cut(s)	1257
PsiI	TTATAA	1 cut(s)	1241
Psp5II	RGGWCCY	1 cut(s)	1489
PspCI	CACGTG	1 cut(s)	5072
PspEI	GGTNACC	1 cut(s)	5329
PspFI	CCCAGC	2 cut(s)	959, 1658
PspPPI	RGGWCCY	1 cut(s)	1489
PstI	CTGCAG	2 cut(s)	3847, 5290
PstNI	CAGNNNCTG	2 cut(s)	1741, 5509
PvuII	CAGCTG	2 cut(s)	562, 3791
RsaI	GTAC	6 cut(s)	2608, 2705, 3754, 3893, 4098, 5739
RsaNI	GTAC	6 cut(s)	2607, 2704, 3753, 3892, 4097, 5738
RseI	CAYNNNNRTG	4 cut(s)	1122, 1159, 2034, 2147
Rsr2I	CGGWCCG	1 cut(s)	5359
RsrII	CGGWCCG	1 cut(s)	5359
SapI	GCTCTTC	5 cut(s)	480, 846, 920, 1665, 3133
SchI	GAGTC	5 cut(s)	3142, 4055, 4546, 4650, 5810
SduI	GDGCHC	4 cut(s)	497, 4132, 4784, 5125
SexAI	ACCWGGT	1 cut(s)	2602
SfcI	CTRYAG	4 cut(s)	2055, 3843, 5286, 5683
SinI	GGWCC	6 cut(s)	1480, 1489, 1557, 2547, 3234, 5359
SmiMI	CAYNNNNRTG	4 cut(s)	1122, 1159, 2034, 2147
SmlI	CTYRAG	5 cut(s)	421, 2108, 3150, 4187, 4627
SmoI	CTYRAG	5 cut(s)	421, 2108, 3150, 4187, 4627
SnaBI	TACGTA	1 cut(s)	4069
SpeI	ACTAGT	1 cut(s)	2327
SphI	GCATGC	1 cut(s)	4171
SseBI	AGGCCT	1 cut(s)	592
SspI	AATATT	3 cut(s)	2768, 3028, 4858
StuI	AGGCCT	1 cut(s)	592
StyI	CCWWGG	5 cut(s)	602, 1665, 3371, 3523, 3544
TaaI	ACNGT	9 cut(s)	35, 215, 894, 2361, 2398, 2611, 3648, 4107, 5440
TaiI	ACGT	5 cut(s)	3973, 4071, 5074, 5281, 5732
TaqII	GACCGA	2 cut(s)	2564, 5045
TatI	WGTACW	2 cut(s)	3891, 4096
TauI	GCSGC	3 cut(s)	392, 3724, 4478
TspGWI	ACGGA	7 cut(s)	1450, 2123, 2512, 2542, 4112, 4452, 5351
Van91I	CCANNNNNTGG	7 cut(s)	1554, 2890, 2981, 3372, 3623, 4109, 4919
Vha464I	CTTAAG	1 cut(s)	4187
VpaK11BI	GGWCC	6 cut(s)	1480, 1489, 1557, 2547, 3234, 5359
VspI	ATTAAT	1 cut(s)	1257
XapI	RAATTY	9 cut(s)	121, 346, 574, 1209, 2291, 2650, 2723, 4862, 5062
XbaI	TCTAGA	1 cut(s)	1619
XceI	RCATGY	5 cut(s)	1505, 2033, 2911, 3100, 4171
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3620
XmaJI	CCTAGG	1 cut(s)	3544
XmiI	GTMKAC	1 cut(s)	3117
XmnI	GAANNNNTTC	2 cut(s)	870, 1086
Zsp2I	ATGCAT	3 cut(s)	4417, 4840, 5355

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.