Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Reverse (-)

15080110 .. 15089980

Gene Structure 9871 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g17920.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5904 bp

ATGGAAGCGGAGGCGGGTAGGGTTGCAGGGAGCAGCTGTGGGGATGGGGGAGAGCCGGTTCGGGCGTCTTACTGGCTGGATGCTTGTGAGGACATTGGTGATTTTGTGGACTTTCCCGAGAATTCGACTGCTCCGGCGGCGGTGGTGGTGAATGGGAATGGTGGCAATCAGGAGGAGGTTGGTGATTTTTTCGGTGGGATTGATCACATTCTTGATAGTATTAAGAATGGAGCTGGACTCCCTGATTCGAAGGGGGATGGGGTGGTTGGGAGTGTTAAGGAGGTGGTGGTTGGGGGGTCTGCTCAGAGTGGGGGTCGAAACTCTGAGGCTGGTTATGGAGAAGGGAGAGTGGCGGTGGTGGCTTGTGCTGCGAAGCTTAATCACAATGGTTCTAGCAAGTATGAGAGTAATGGGAGGGGGAATCGGAGTGTGAGGGAAAGAGATGGGAGTGGTGAAGAAAGGTGTCCAAAGAGGGTTGCTGTAGATAATGGTAGGAATGAGAGGTATAGTTCAGGCAGAGGGCAATATCAGATACGGGAGAGGAATTCTGGTCGGAAGAGGCCTCGTGATCCTCGCGACGACATTGATGGTAGGGATAGGGATAGGGATAGGGATAGGGATAGGGATAGGGATGGGGATAGGGATAGGGATAGGGATAGGGATAGGGATAGGGATAGGGACAGAGATAGGGACAGGGATAGAGAACGGACTAGGAGAAGAGAGTGTTATGGTAGTAATAGGAGGGATAGCAGAGATTTCGAAGCAAAGGGTTATTGGGAGAGGGATAAATTGGGGTCAAATGAGCTTGTTTTTAGATTAGGAACTTACGAACCTCATCAGAAGAAAGAGGAGAAAGTTGCCACTGACAAAACCAATGAAAAGGATGTGAAGAAATCTGAAGAGCTGAAGAAAGAAAAGATTCCCGAGGAACAAGCCAGACAGTATCAATTGGATGTTCTTGAACAGGCAAAGAAGAACAATACGATAGCTTTTTTAGAAACTGGGGCAGGGAAGACACTCATTGCTATTCTCCTCATGCAAAGTGTCTGCAATGATTTGGAAAAGAAGAATAAAAAGATGCTTGCTGTGTTTCTAGTTCCTAAAGTTCCACTTGTTTATCAGCAAGCAGAAGTTATTCGTGAACGAACTGGTTTCCAAGTGGGCCATTATTGTGGTGAGATGGGTCAAGACTTTTGGGATACACGAAAATGGCAGCGTGAGTTTGATACGAAACAGGTTCTAGTAATGACAGCTCAAATTTTATTGAACATTCTAAGACACAGCATTATAAGGATGGATTCGATTAGTCTATTGATTCTGGATGAGTGTCATCACGCTGTGAAGAAACATCCGTACTCTTTAGTGATGTCTGAGTTCTATCATACAACCCCAAAAGAGAAGAGACCATCTATATTTGGAATGACTGCTTCTCCTGTTAACTTAAAAGGTGTTTCCAATCAACTAGATTGTGCAATAAAGATTCGTAATCTTGAAAGTAAACTGGATTCCGTTGTTTGCACCATAAAGGATCGTAAGGACCTGGAAAAGCATGTTCCGATGCCCTCAGAAATTGTTGTGGAGTATGACAAAGCAGCCAGCTTATGCTCCTTGCATGAACAGCTAAAACAAATGGAATTGGAAGTTGAAGAAGCTGCAAAGTCTAGCTCTAGAAGAAGTAAATGGCAGTTTATGGGAGCTAGAGATGCCGGGGCCAAGGAAGAGCTACGCCAAGTGTATGGTGTTTCAGAAAGAACAGAAAGTGATGGGGCTGTTAACCTAATTCAGAAGCTGAGGGCTATCAACTATGCACTTGGTGAACTGGGCCAGTGGTGTGCTTACAAGGTGGCGCAATCATTTTTGACAGCTTTACAAAATGATGAGAGAGCAAACTACCAGCTTGATGTCAAGTTTCAAGAAAATTACCTGATAAGAGTCGCATCTATTTTACAATGTCATCTGTCAGAGGGCGCTGCTTCTGACAAGGAAACAAATCTACCAGATTCAGAGAGTGGTGTTTCTCATGATGAGATCGAGGAAGGAGAGCTACCCGATAGTCATGTTGTTTCTGTTGGGGAACATGTAGATGTGATAATAGGAGCTGCTGTAGCTGATGGGAAGGTGACGCCAAAAGTGCAGTCACTAATTAAAATTCTTCTCAAGTATCAGCATACAGAAGATTTCCGTGCAATCATTTTTGTGGAGCGAGTTGTCTCTGCTTTAGTCCTTCCTAAGGTCTTTGCTGAGCTTCCATCTCTAGGCTTTATCGAGTGTGCTAGCTTGATTGGCCACAATAATAGTCAGGAAATGCGGAGTAGTCAAATGCAGGACACAATTGCCAAATTCAAGGACGGTCGTGTGACTTTGTTAGTTGCAACTAGTGTTGCTGAGGAAGGACTTGATATTCGACAGTGCAATGTTGTTATTCGCTTTGACCTTGCAAAAACTGTTTTGGCATATATTCAGTCTAGGGGCCGTGCTAGAAAACCTGGGTCAGATTATATCTTGATGGTGGAAAGGGGAAATCTGTCACATGAAGCATTTTTACGGAATGCCAGAAATAGTGAGGAGACTTTACGGAGAGAAGCAATAGAGAGGACGGACCTGAGTGATTTGAAGGATAGTTCAAGGTTAATTTCAGTGGAAACTGCACCAGGTACAGTGTACCAGGTGGAGTCAACTGGTGCTCTCGTGAGCTTAAATTCTGCTGTTGGATTAATCCATTTCTATTGCTCTCAGCTTCCGAGTGACAGGTACTCAATACTTCATCCTGAGTTTGTAATGGTGAGACATGAGAAGCAAGGAGGCCCGACTGAATATTCATGCAAGCTTCAACTCCCTTGTAATGCACCGTTTGAGACACTTGAAGGCCCTGTGTGCAGTTCAATGCACCTCGCTCAACAGGCTGTTTGTTTGGCGGCCTGTAAGAAACTTCATGAGATGGGAGCATTTACTGACATGCTCTTGCCCGACAGGGGAGTTGGGGAAGAAAAAGAAAAGGTTGACAAGAATGACGAAGGAGATCCGCTTCCTGGAACTGCTAGGCACAGAGAGTTCTATCCCGAGGGCGTAGCTAACATTCTCCAGGGAGAGTGGATCTTAGCTGGAAAAGATCTTGGCAATGAGGCGAAATTGATTAATGTTTACATGTATTCCGTGAAATGTGTAGACATTGGCTCTTCAAAAGATCCTTTCTTGACTCAAGTTTCAGATTTTGCAGTTCTATTAGGCAATGAGCTGGATGCAGAGGTGTTATCGATGTCGATGGATCTATTTGTTGCTCGGACCATGACTACAAAGGCTTCCCTTGCCTTCAGAGGTTCAATTAGTATCACTGAAAGTCAGTTGGCATCCCTAAAAAGTTTCCATGTAAGATTGATGAGTATTGTACTGGATGTGGATGTTGAACCTTCCACCACTCCTTGGGATCCTGCAAAGGCTTATTTATTTGTCCCCGTGGTTAGTGATAACTGTGGAGATGCTATGAAAGAAATTGATTGGGATCTGGTTGAAAATATAATTGGTGCGAATGCGTGGAACAATCCCCTTCAAAGAGCTAGGCCTGATGTTTTCCTTGGCACAAATGAACGAACCCTAGGAGGTGACAGAAGGGAGTATGGTTTCGCTAAATTGCGTCATGGCATGGTTCATGGGCAAAAATCCCATCCAACCTACGGTATCAGAGGAGCTGTGGCACAGTTTGATGTCGTTAAAGCTTCTGGACTGATCCCTGATCGGGATGCATTTGAAATGCAAAAGGATGTGGATCTGCCTCAACACAAGTTGATGATGGCAGATAGTTGTACCAAGGTCGAAGATTTGGTCGGGAAAATTGTCACAGCTGCTCACTCGGGGAAGAGATTTTATGTTGATTCAATATGCTATGATATGACTGCAGAAAACTCTTTCCCGAGGAAAGAAGGTTATCTCGGTCCTTTGGAGTACAGCTCATATGCTGATTATTACAAGCAAAAGTATGGAGTTCAGTTAATGTATAAGAAACAACCTCTAATAAAAGGACGTGGTGTCTCATATTGTAAGAATCTCCTGTCCCCTCGGTTTGACCACGTGGAAGAACTTGAAGGTGAATCTGGAGAGTCTCTGGACAAAACATACTATGTATTTCTCCCTCCTGAGCTTTGTTTAGTACACCCACTGTCTGGATCACTTGTCCGTGGAGCTCAAAGATTACCCTCAATAATGAAAAGGGTTGAAAGCATGCTGCTAGCGGTTGAACTTAAGGAGATAATAAATTATCCTGTCCCTGCTTCTAAGATATTGGAAGCATTGACTGCTGCTTCATGCCAGGAAACATTTTGCTATGAAAGAGCGGAGCTTCTGGGTGATGCATACCTCAAATGGGTTGTTAGTCGATTTCTTTTTCTTAAGTACCCTCAGAAACACGAAGGTCAGCTTACTAGGATGAGACAACAAAAGGTGAGTAACATGGTTTTGTATCACCATGCATTGGAAAGAGGACTTCAATCATATATCCAAGCAGATCGCTTTGCTCCATCTCGATGGGCGGCTCCTGGGGTGTTGCCCGTCTTTGATGAATATACAAAAGATGAAGAATCATCCTTGTTTGACCAAGAGGATGTAAATCGACGAAAAACAGATGACCCGATTAATGAATATGAGGATGATGAATTGGAGGATGGCGAGCTGGAGAGCGACTTGAGTTCTTATAGAGTCTTGTCAAGCAAGACACTTGCAGATGTTGTTGAAGCGCTTATTGGGGTTTATTACGTAGAAGGTGGGAAAAATGCTGCTAACCATCTCATGAAATGGGTTGGAATTGATGTAGAGTTTAATGCTGATGAGATAGAGAACACAACAAGGCCATGTAATGTTCCCGACAGCGTTCTTAGGAGCATTGACTTTGATGCGTTGGAGGGTGCTTTGAATATTAAATTTAGAGACAAGGGCCTCTTGGTGGAAGCCATTAGTCATGCTTCACGACCATCTTCTGGAGTAGCCTGCTATCAGCGGTTGGAGTTTGTGGGTGATGCTGTCTTAGATCATCTTATTACAAAACACTTGTTCTTCACATACACAGATCTGCCTCCAGGCAGGTTGACCGATCTTAGAGCTGCTGCTGTTAACAATGAAAATTTTGCTCGTGTTGCTGTGAAACATAATCTCCATCTACACCTTCGACATGGATCCAGTGCTCTTGAAAGACAGATCCATGACTTTGTGAAGGAAGCTGCAAATGAATTAACAAAGCCTGGGCTCAACTCATTTGGTTTGGGAGATTGTAAAGCTCCTAAAGTTCTTGGTGACATTATCGAATCTATTGCGGGTGCCATTTTTCTAGATAGTGGACGTAATACTGCAGTTGTTTGGAAGGTGTTTGAACCTCTGCTTCAACCAATGGTCACCCCAGAGACACTACCAATGCATCCGGTCCGTGAGCTGCAAGAAAGATGCCAGCAACAGGCTGAAGGCCTTGAATACAAAGCCAGTCGAAGTGGCAATTTGGCTACTGTTGAAGTCTTGATCGATGGTGTCAAGGTGGGAATTGCTCAGAATCCACAAAAGAAGATGGCACAGAAACTGGCTGCCAGGAATGCTCTTGCTGCTTTAAAAGATAAGGAAACAGCTGAAGCAAAGGAGAGGCAGGAGGAAGATAATGGGAAGAAGAAGAAAAATGGTAGCCAAACATTTACCAGGCAAACTTTAAATGATATATGCTTGCGTAAAAACTGGCCCATGCCATTCTACAGGTGTGTCAATGAGGGTGGCCCTGCACACGCAAAGAAGTTTACATTTGCTGTACGCGTGAATACTACTGACAGAGGATGGATAGACGAATGTATCGGAGAGCCTATGCCGAGTGTCAAGAAGGCCAAGGACTCGGCAGCAGTTCTTCTATTGGAACTATTGAATAAGTTATACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1968

Amino Acids

220.26

Weight (kDa)

5.89

Isoelectric Point (pI)

40.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	4 - 133	1.5e-09	DEAD/DEAH box helicase
ResIII	PF04851	4 - 133	1.1e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	365 - 482	8.7e-17	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	557 - 645	3.5e-22	Dicer dimerisation domain
PAZ	PF02170	926 - 1058	3.1e-18	PAZ domain
Ribonucleas_3_3	PF14622	1083 - 1154	1.7e-06	Ribonuclease-III-like
Ribonuclease_3	PF00636	1096 - 1241	8.6e-20	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1295 - 1435	1.2e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1317 - 1430	1.4e-22	Ribonuclease III domain
dsrm	PF00035	1457 - 1517	8.2e-06	Double-stranded RNA binding motif
DND1_DSRM	PF14709	1544 - 1617	1.7e-15	double strand RNA binding domain from DEAD END PROTEIN 1
dsrm	PF00035	1547 - 1617	6.7e-08	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1289
Acc36I	ACCTGC	1 cut(s)	5061
AccB1I	GGYRCC	1 cut(s)	5303
AccB7I	CCANNNNNTGG	4 cut(s)	3420, 4157, 4465, 4967
AccBSI	CCGCTC	1 cut(s)	4328
AccI	GTMKAC	1 cut(s)	3165
AccII	CGCG	2 cut(s)	576, 5781
AcoI	YGGCCR	1 cut(s)	2284
AcsI	RAATTY	8 cut(s)	121, 544, 1257, 2148, 2339, 2698, 4910, 5110
AcuI	CTGAAG	5 cut(s)	918, 926, 3295, 5463, 5625
AcvI	CACGTG	1 cut(s)	4066
AcyI	GRCGYC	2 cut(s)	65, 2123
AdeI	CACNNNGTG	2 cut(s)	1339, 1814
AfeI	AGCGCT	1 cut(s)	4728
AflII	CTTAAG	2 cut(s)	4235, 4382
AflIII	ACRYGT	3 cut(s)	2077, 3144, 5779
AhdI	GACNNNNNGTC	1 cut(s)	4022
AhlI	ACTAGT	1 cut(s)	2375
AjiI	CACGTC	1 cut(s)	4019
AjuI	GAANNNNNNNTTGG	4 cut(s)	3501, 3533, 4716, 4748
AloI	GAACNNNNNNTCC	2 cut(s)	42, 74
Alw21I	GWGCWC	3 cut(s)	2686, 4180, 5173
AlwNI	CAGNNNCTG	2 cut(s)	1789, 5557
Ama87I	CYCGRG	5 cut(s)	116, 923, 3059, 3847, 3907
Aor51HI	AGCGCT	1 cut(s)	4728
ApoI	RAATTY	8 cut(s)	121, 544, 1257, 2148, 2339, 2698, 4910, 5110
ArsI	GACNNNNNNTTYG	6 cut(s)	2120, 2152, 2385, 2417, 3804, 3836
AseI	ATTAAT	3 cut(s)	2714, 3135, 4626
Asp700I	GAANNNNTTC	2 cut(s)	918, 1134
AspA2I	CCTAGG	1 cut(s)	3592
AspLEI	GCGC	3 cut(s)	1849, 1970, 4729
AsuC2I	CCSGG	1 cut(s)	1708
AsuII	TTCGAA	2 cut(s)	248, 759
AsuNHI	GCTAGC	2 cut(s)	2273, 4222
AvaI	CYCGRG	5 cut(s)	116, 923, 3059, 3847, 3907
AvaII	GGWCC	5 cut(s)	1537, 2599, 3282, 3929, 5407
AvrII	CCTAGG	1 cut(s)	3592
AxyI	CCTNAGG	1 cut(s)	2229
BaeI	ACNNNNGTAYC	2 cut(s)	5800, 5833
BalI	TGGCCA	1 cut(s)	2286
BamHI	GGATCC	2 cut(s)	3424, 5162
BanI	GGYRCC	1 cut(s)	5303
BanII	GRGCYC	2 cut(s)	4180, 5235
BarI	GAAGNNNNNNTAC	4 cut(s)	2028, 2060, 2526, 2558
BauI	CACGAG	3 cut(s)	564, 2687, 5118
BbrPI	CACGTG	1 cut(s)	4066
BbsI	GAAGAC	1 cut(s)	1019
Bbv12I	GWGCWC	3 cut(s)	2686, 4180, 5173
BbvCI	CCTCAGC	2 cut(s)	1790, 2385
BceAI	ACGGC	1 cut(s)	2457
BcgI	CGANNNNNNTGC	4 cut(s)	739, 773, 5279, 5313
BciVI	GTATCC	1 cut(s)	1192
BclI	TGATCA	1 cut(s)	202
BcnI	CCSGG	1 cut(s)	1708
BcuI	ACTAGT	1 cut(s)	2375
BfmI	CTRYAG	5 cut(s)	480, 2103, 3891, 5334, 5722
BfoI	RGCGCY	2 cut(s)	1971, 4730
BfrI	CTTAAG	2 cut(s)	4235, 4382
BfuAI	ACCTGC	1 cut(s)	5061
BfuI	GTATCC	1 cut(s)	1192
BglII	AGATCT	2 cut(s)	3109, 5056
BlnI	CCTAGG	1 cut(s)	3592
BlpI	GCTNAGC	1 cut(s)	2241
Bme18I	GGWCC	5 cut(s)	1537, 2599, 3282, 3929, 5407
BmeRI	GACNNNNNGTC	1 cut(s)	4022
BmeT110I	CYCGRG	5 cut(s)	116, 923, 3059, 3847, 3907
BmgBI	CACGTC	1 cut(s)	4019
BmiI	GGNNCC	6 cut(s)	1711, 2471, 3426, 4527, 5164, 5305
BmrI	ACTGGG	2 cut(s)	1011, 1829
BmtI	GCTAGC	2 cut(s)	2277, 4226
BmuI	ACTGGG	2 cut(s)	1011, 1829
BpiI	GAAGAC	1 cut(s)	1019
BplI	GAGNNNNNCTC	4 cut(s)	222, 254, 3929, 3961
BpmI	CTGGAG	5 cut(s)	3065, 4110, 4685, 4989, 5049
Bpu10I	CCTNAGC	3 cut(s)	1790, 2385, 4131
Bpu1102I	GCTNAGC	1 cut(s)	2241
Bpu14I	TTCGAA	2 cut(s)	248, 759
BpuEI	CTTGAG	3 cut(s)	2141, 3183, 4696
BpuMI	CCSGG	1 cut(s)	1708
Bsa29I	ATCGAT	2 cut(s)	3254, 5502
BsaAI	YACGTR	2 cut(s)	4066, 4747
BsaBI	GATNNNNATC	3 cut(s)	3498, 3762, 4599
BsaHI	GRCGYC	2 cut(s)	65, 2123
BsaI	GGTCTC	1 cut(s)	1396
BsaWI	WCCGGW	1 cut(s)	5404
Bse118I	RCCGGY	1 cut(s)	55
Bse21I	CCTNAGG	1 cut(s)	2229
Bse3DI	GCAATG	5 cut(s)	1020, 1057, 2419, 3124, 3235
Bse8I	GATNNNNATC	3 cut(s)	3498, 3762, 4599
BseCI	ATCGAT	2 cut(s)	3254, 5502
BseJI	GATNNNNATC	3 cut(s)	3498, 3762, 4599
BseMI	GCAATG	5 cut(s)	1020, 1057, 2419, 3124, 3235
BseRI	GAGGAG	5 cut(s)	188, 863, 1022, 2579, 3696
BsgI	GTGCAG	4 cut(s)	2153, 2631, 2896, 5733
Bsh1236I	CGCG	2 cut(s)	576, 5781
Bsh1285I	CGRYCG	1 cut(s)	2353
BshNI	GGYRCC	1 cut(s)	5303
BshVI	ATCGAT	2 cut(s)	3254, 5502
BsiEI	CGRYCG	1 cut(s)	2353
BsiHKAI	GWGCWC	3 cut(s)	2686, 4180, 5173
BsiHKCI	CYCGRG	5 cut(s)	116, 923, 3059, 3847, 3907
BsiSI	CCGG	4 cut(s)	56, 134, 1707, 5405
BslFI	GGGAC	5 cut(s)	692, 704, 3434, 4033, 4244
BsmFI	GGGAC	5 cut(s)	692, 704, 3434, 4033, 4244
BsmI	GAATGC	3 cut(s)	2554, 3532, 5575
Bso31I	GGTCTC	1 cut(s)	1396
BsoBI	CYCGRG	5 cut(s)	116, 923, 3059, 3847, 3907
Bsp119I	TTCGAA	2 cut(s)	248, 759
Bsp1286I	GDGCHC	4 cut(s)	2686, 4180, 5173, 5235
Bsp1720I	GCTNAGC	1 cut(s)	2241
Bsp68I	TCGCGA	1 cut(s)	576
BspDI	ATCGAT	2 cut(s)	3254, 5502
BspFNI	CGCG	2 cut(s)	576, 5781
BspHI	TCATGA	3 cut(s)	2020, 2932, 4779
BspLI	GGNNCC	6 cut(s)	1711, 2471, 3426, 4527, 5164, 5305
BspMAI	CTGCAG	2 cut(s)	3895, 5338
BspMI	ACCTGC	1 cut(s)	5061
BspOI	GCTAGC	2 cut(s)	2277, 4226
BspQI	GCTCTTC	3 cut(s)	894, 1713, 3181
BspT104I	TTCGAA	2 cut(s)	248, 759
BspT107I	GGYRCC	1 cut(s)	5303
BspTI	CTTAAG	2 cut(s)	4235, 4382
BspTNI	GGTCTC	1 cut(s)	1396
BsrBI	CCGCTC	1 cut(s)	4328
BsrDI	GCAATG	5 cut(s)	1020, 1057, 2419, 3124, 3235
BsrFI	RCCGGY	1 cut(s)	55
BssAI	RCCGGY	1 cut(s)	55
BssNI	GRCGYC	2 cut(s)	65, 2123
BssSI	CACGAG	3 cut(s)	564, 2687, 5118
BssT1I	CCWWGG	6 cut(s)	1713, 3419, 3571, 3592, 3804, 5850
Bst2BI	CACGAG	3 cut(s)	564, 2687, 5118
Bst6I	CTCTTC	7 cut(s)	551, 712, 894, 1394, 1713, 3181, 3848
BstACI	GRCGYC	2 cut(s)	65, 2123
BstAFI	CTTAAG	2 cut(s)	4235, 4382
BstAPI	GCANNNNNTGC	1 cut(s)	4289
BstBAI	YACGTR	2 cut(s)	4066, 4747
BstBI	TTCGAA	2 cut(s)	248, 759
BstDSI	CCRYGG	2 cut(s)	3453, 4171
BstEII	GGTNACC	1 cut(s)	5377
BstENI	CCTNNNNNAGG	1 cut(s)	2228
BstFNI	CGCG	2 cut(s)	576, 5781
BstH2I	RGCGCY	2 cut(s)	1971, 4730
BstHHI	GCGC	3 cut(s)	1849, 1970, 4729
BstMCI	CGRYCG	1 cut(s)	2353
BstNSI	RCATGY	5 cut(s)	1553, 2081, 2959, 3148, 4219
BstPI	GGTNACC	1 cut(s)	5377
BstSFI	CTRYAG	5 cut(s)	480, 2103, 3891, 5334, 5722
BstSNI	TACGTA	1 cut(s)	4747
BstUI	CGCG	2 cut(s)	576, 5781
BstV2I	GAAGAC	1 cut(s)	1019
BstXI	CCANNNNNNTGG	3 cut(s)	1172, 1736, 4518
Bsu15I	ATCGAT	2 cut(s)	3254, 5502
Bsu36I	CCTNAGG	1 cut(s)	2229
BsuI	GTATCC	1 cut(s)	1192
BsuTUI	ATCGAT	2 cut(s)	3254, 5502
BtgI	CCRYGG	2 cut(s)	3453, 4171
BtrI	CACGTC	1 cut(s)	4019
BtsIMutI	CAGTG	8 cut(s)	861, 1832, 2414, 2643, 2664, 3330, 4151, 5173
BtuMI	TCGCGA	1 cut(s)	576
BveI	ACCTGC	1 cut(s)	5061
CaiI	CAGNNNCTG	2 cut(s)	1789, 5557
CciI	TCATGA	3 cut(s)	2020, 2932, 4779
CfoI	GCGC	3 cut(s)	1849, 1970, 4729
Cfr10I	RCCGGY	1 cut(s)	55
ClaI	ATCGAT	2 cut(s)	3254, 5502
CpoI	CGGWCCG	1 cut(s)	5407
CseI	GACGC	3 cut(s)	54, 2131, 3620
CsiI	ACCWGGT	2 cut(s)	2650, 2664
CspCI	CAANNNNNGTGG	2 cut(s)	5722, 5757
CspI	CGGWCCG	1 cut(s)	5407
DraI	TTTAAA	2 cut(s)	5586, 5682
DraIII	CACNNNGTG	2 cut(s)	1339, 1814
DriI	GACNNNNNGTC	1 cut(s)	4022
EaeI	YGGCCR	1 cut(s)	2284
Eam1104I	CTCTTC	7 cut(s)	551, 712, 894, 1394, 1713, 3181, 3848
Eam1105I	GACNNNNNGTC	1 cut(s)	4022
EarI	CTCTTC	7 cut(s)	551, 712, 894, 1394, 1713, 3181, 3848
Ecl136II	GAGCTC	1 cut(s)	4178
Eco105I	TACGTA	1 cut(s)	4747
Eco130I	CCWWGG	6 cut(s)	1713, 3419, 3571, 3592, 3804, 5850
Eco147I	AGGCCT	3 cut(s)	562, 3559, 5448
Eco24I	GRGCYC	2 cut(s)	4180, 5235
Eco31I	GGTCTC	1 cut(s)	1396
Eco47I	GGWCC	5 cut(s)	1537, 2599, 3282, 3929, 5407
Eco47III	AGCGCT	1 cut(s)	4728
Eco53kI	GAGCTC	1 cut(s)	4178
Eco57I	CTGAAG	5 cut(s)	918, 926, 3295, 5463, 5625
Eco72I	CACGTG	1 cut(s)	4066
Eco81I	CCTNAGG	1 cut(s)	2229
Eco88I	CYCGRG	5 cut(s)	116, 923, 3059, 3847, 3907
Eco91I	GGTNACC	1 cut(s)	5377
EcoICRI	GAGCTC	1 cut(s)	4178
EcoNI	CCTNNNNNAGG	1 cut(s)	2228
EcoO109I	RGGNCCY	3 cut(s)	1537, 2867, 4924
EcoO65I	GGTNACC	1 cut(s)	5377
EcoRI	GAATTC	2 cut(s)	121, 544
EcoT14I	CCWWGG	6 cut(s)	1713, 3419, 3571, 3592, 3804, 5850
EcoT22I	ATGCAT	4 cut(s)	3742, 4348, 4465, 5403
EcoT38I	GRGCYC	2 cut(s)	4180, 5235
ErhI	CCWWGG	6 cut(s)	1713, 3419, 3571, 3592, 3804, 5850
FalI	AAGNNNNNCTT	6 cut(s)	1095, 1127, 4395, 4427, 4913, 4945
FaqI	GGGAC	5 cut(s)	692, 704, 3434, 4033, 4244
FauI	CCCGC	2 cut(s)	7, 5293
FauNDI	CATATG	1 cut(s)	3949
FbaI	TGATCA	1 cut(s)	202
FblI	GTMKAC	1 cut(s)	3165
FriOI	GRGCYC	2 cut(s)	4180, 5235
GlaI	GCGC	3 cut(s)	1848, 1969, 4728
GsuI	CTGGAG	5 cut(s)	3065, 4110, 4685, 4989, 5049
HaeII	RGCGCY	2 cut(s)	1971, 4730
HapII	CCGG	4 cut(s)	56, 134, 1707, 5405
HgaI	GACGC	3 cut(s)	54, 2131, 3620
HhaI	GCGC	3 cut(s)	1849, 1970, 4729
Hin1I	GRCGYC	2 cut(s)	65, 2123
Hin6I	GCGC	3 cut(s)	1847, 1968, 4727
HinP1I	GCGC	3 cut(s)	1847, 1968, 4727
HincII	GTYRAC	6 cut(s)	1438, 1774, 2676, 3001, 5076, 5101
HindII	GTYRAC	6 cut(s)	1438, 1774, 2676, 3001, 5076, 5101
HindIII	AAGCTT	3 cut(s)	374, 2825, 3711
HpaI	GTTAAC	3 cut(s)	1438, 1774, 5101
HpaII	CCGG	4 cut(s)	56, 134, 1707, 5405
Hpy99I	CGWCG	2 cut(s)	581, 4608
HpyCH4IV	ACGT	4 cut(s)	4018, 4065, 4746, 5326
HpySE526I	ACGT	4 cut(s)	4018, 4065, 4746, 5326
Hsp92I	GRCGYC	2 cut(s)	65, 2123
HspAI	GCGC	3 cut(s)	1847, 1968, 4727
Ksp22I	TGATCA	1 cut(s)	202
KspAI	GTTAAC	3 cut(s)	1438, 1774, 5101
LguI	GCTCTTC	3 cut(s)	894, 1713, 3181
MabI	ACCWGGT	2 cut(s)	2650, 2664
MaeII	ACGT	4 cut(s)	4018, 4065, 4746, 5326
MbiI	CCGCTC	1 cut(s)	4328
MfeI	CAATTG	2 cut(s)	947, 2331
MhlI	GDGCHC	4 cut(s)	2686, 4180, 5173, 5235
MlsI	TGGCCA	1 cut(s)	2286
MluI	ACGCGT	1 cut(s)	5779
MluNI	TGGCCA	1 cut(s)	2286
MlyI	GAGTC	7 cut(s)	231, 1941, 2681, 3190, 4103, 4698, 5849
MmeI	TCCRAC	6 cut(s)	533, 2689, 3689, 4771, 4869, 4971
Mox20I	TGGCCA	1 cut(s)	2286
Mph1103I	ATGCAT	4 cut(s)	3742, 4348, 4465, 5403
MroXI	GAANNNNTTC	2 cut(s)	918, 1134
MscI	TGGCCA	1 cut(s)	2286
MslI	CAYNNNNRTG	5 cut(s)	1170, 1207, 2082, 2195, 4516
Msp20I	TGGCCA	1 cut(s)	2286
MspA1I	CMGCKG	4 cut(s)	36, 3839, 4987, 5603
MspCI	CTTAAG	2 cut(s)	4235, 4382
MspI	CCGG	4 cut(s)	56, 134, 1707, 5405
MunI	CAATTG	2 cut(s)	947, 2331
Mva1269I	GAATGC	3 cut(s)	2554, 3532, 5575
MvnI	CGCG	2 cut(s)	576, 5781
NciI	CCSGG	1 cut(s)	1708
NdeI	CATATG	1 cut(s)	3949
NheI	GCTAGC	2 cut(s)	2273, 4222
NlaIV	GGNNCC	6 cut(s)	1711, 2471, 3426, 4527, 5164, 5305
NmeAIII	GCCGAG	2 cut(s)	5837, 5859
NmuCI	GTSAC	9 cut(s)	2119, 2136, 2356, 2526, 2744, 3599, 3832, 5279, 5377
NruI	TCGCGA	1 cut(s)	576
NsiI	ATGCAT	4 cut(s)	3742, 4348, 4465, 5403
NspI	RCATGY	5 cut(s)	1553, 2081, 2959, 3148, 4219
NspV	TTCGAA	2 cut(s)	248, 759
PaeI	GCATGC	1 cut(s)	4219
PagI	TCATGA	3 cut(s)	2020, 2932, 4779
PceI	AGGCCT	3 cut(s)	562, 3559, 5448
PciI	ACATGT	2 cut(s)	2077, 3144
PciSI	GCTCTTC	3 cut(s)	894, 1713, 3181
PctI	GAATGC	3 cut(s)	2554, 3532, 5575
PdmI	GAANNNNTTC	2 cut(s)	918, 1134
PflMI	CCANNNNNTGG	4 cut(s)	3420, 4157, 4465, 4967
PfoI	TCCNGGA	1 cut(s)	3028
PleI	GAGTC	7 cut(s)	231, 1940, 2680, 3190, 4102, 4697, 5849
PmaCI	CACGTG	1 cut(s)	4066
PmlI	CACGTG	1 cut(s)	4066
PpsI	GAGTC	7 cut(s)	231, 1940, 2680, 3190, 4102, 4697, 5849
Ppu21I	YACGTR	2 cut(s)	4066, 4747
PpuMI	RGGWCCY	1 cut(s)	1537
PscI	ACATGT	2 cut(s)	2077, 3144
PshBI	ATTAAT	3 cut(s)	2714, 3135, 4626
PsiI	TTATAA	1 cut(s)	1289
Psp124BI	GAGCTC	1 cut(s)	4180
Psp5II	RGGWCCY	1 cut(s)	1537
PspCI	CACGTG	1 cut(s)	4066
PspEI	GGTNACC	1 cut(s)	5377
PspN4I	GGNNCC	6 cut(s)	1711, 2471, 3426, 4527, 5164, 5305
PspPPI	RGGWCCY	1 cut(s)	1537
PstI	CTGCAG	2 cut(s)	3895, 5338
PstNI	CAGNNNCTG	2 cut(s)	1789, 5557
PvuII	CAGCTG	3 cut(s)	36, 3839, 5603
RruI	TCGCGA	1 cut(s)	576
RseI	CAYNNNNRTG	5 cut(s)	1170, 1207, 2082, 2195, 4516
Rsr2I	CGGWCCG	1 cut(s)	5407
RsrII	CGGWCCG	1 cut(s)	5407
SacI	GAGCTC	1 cut(s)	4180
SapI	GCTCTTC	3 cut(s)	894, 1713, 3181
SchI	GAGTC	7 cut(s)	231, 1941, 2681, 3190, 4103, 4698, 5849
SduI	GDGCHC	4 cut(s)	2686, 4180, 5173, 5235
SexAI	ACCWGGT	2 cut(s)	2650, 2664
SfcI	CTRYAG	5 cut(s)	480, 2103, 3891, 5334, 5722
SfuI	TTCGAA	2 cut(s)	248, 759
SinI	GGWCC	5 cut(s)	1537, 2599, 3282, 3929, 5407
SmiMI	CAYNNNNRTG	5 cut(s)	1170, 1207, 2082, 2195, 4516
SmlI	CTYRAG	5 cut(s)	2156, 3198, 4235, 4382, 4675
SmoI	CTYRAG	5 cut(s)	2156, 3198, 4235, 4382, 4675
SnaBI	TACGTA	1 cut(s)	4747
SpeI	ACTAGT	1 cut(s)	2375
SphI	GCATGC	1 cut(s)	4219
SseBI	AGGCCT	3 cut(s)	562, 3559, 5448
SspI	AATATT	2 cut(s)	2816, 4906
SstI	GAGCTC	1 cut(s)	4180
StuI	AGGCCT	3 cut(s)	562, 3559, 5448
StyI	CCWWGG	6 cut(s)	1713, 3419, 3571, 3592, 3804, 5850
TaiI	ACGT	4 cut(s)	4021, 4068, 4749, 5329
TaqII	GACCGA	2 cut(s)	3917, 5093
TatI	WGTACW	3 cut(s)	3385, 3939, 4144
TauI	GCSGC	3 cut(s)	140, 2918, 4526
TscAI	CASTG	8 cut(s)	868, 1832, 2414, 2643, 2664, 3337, 4158, 5173
TseFI	GTSAC	9 cut(s)	2119, 2136, 2356, 2526, 2744, 3599, 3832, 5279, 5377
Tsp45I	GTSAC	9 cut(s)	2119, 2136, 2356, 2526, 2744, 3599, 3832, 5279, 5377
TspRI	CASTG	8 cut(s)	868, 1832, 2414, 2643, 2664, 3337, 4158, 5173
Van91I	CCANNNNNTGG	4 cut(s)	3420, 4157, 4465, 4967
Vha464I	CTTAAG	2 cut(s)	4235, 4382
VpaK11BI	GGWCC	5 cut(s)	1537, 2599, 3282, 3929, 5407
VspI	ATTAAT	3 cut(s)	2714, 3135, 4626
XagI	CCTNNNNNAGG	1 cut(s)	2228
XapI	RAATTY	8 cut(s)	121, 544, 1257, 2148, 2339, 2698, 4910, 5110
XbaI	TCTAGA	2 cut(s)	1667, 5314
XceI	RCATGY	5 cut(s)	1553, 2081, 2959, 3148, 4219
XmaJI	CCTAGG	1 cut(s)	3592
XmiI	GTMKAC	1 cut(s)	3165
XmnI	GAANNNNTTC	2 cut(s)	918, 1134
Zsp2I	ATGCAT	4 cut(s)	3742, 4348, 4465, 5403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.