Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Reverse (-)

53133662 .. 53144809

Gene Structure 11148 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG299700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5871 bp

ATGGATACAGAGCCGGCTAGGGTTACAGGGGACAGTGGTGGCAATGGGGGAGAGGCGGTTCGGGCTTCTTACTGGCTGGATGCTTGTGAGGACATTGGGGATTTTGTGGACTTTTCCGAAAATTCGGGTGCTACGGTAGTGGTGGCGAGTGGGAATGGTTGCAATCAGGAGGAGGTGGGTGATTTTTTCGGCGGGATTGATCACATTCTTGACAGTATCAAAAGCGGAGCTGGATTGCCGGATGGGAAGGGGGATGGGGTAGTGAGCAGTGGCAATGCGGAGGAGGTTGGGGCGACTGGCGTTTCCAATGGACTGAGTGGTGAGAATGGTCAGAGTGGGGGTCGAAATTCTGAGGGTGCTAATGGAGATGGGAGAGTGGCGGTGGTGGTGGCTTCTGCTGCTAAGCGTAATCACAATGGTTTGAGCAAGTATGAGAGTAATGGGAGGTGGAATCGGGGTGTGAGGGAAAGAGATGTGAGTGGTGAAGAGCGGTGCCCCAAGAGGGTTGCGGTAGATAATGGTAGGAATGAGAGGTATAGTTCAGGCAGAGTGCAATATCAGCTGAGGGAGAGGAATTCTGGTCGGAAGAGGCCTCGTGATCCTCGGGATGACATTGATAGGAGGGATAGGGATAGGGACAGAGATAGGGACAGGGATAGAGATCGGACTAGGAGAAGAGAGAGTTATGGTAGTAATAGGAGGGATAGTAGAGATTGGGAAGCAAAGGGCTATTGGGAGAGGGATAAATTGGGGTCAAATGAGCTTGTTTTTAGATTAGGAGCTTATGAACCTCATCAGAAGAAAGAGGAGAAAGTCGCTAATGACAAAATCAATGAAAAGGATGTGAAGAAATCTGAAGAGCTGAAGAAAGAAAAGATTCCTGAGGAACGAGCCAGACAATATCAATTGGATGTTCTTGAACAGGCAAAGAAGAGCAATACGATAGCTTTTCTTGAAACTGGGGCTGGGAAGACACTCATTGCCATTCTTCTCATGCAAAGTGTCTGCAATGATTTGCAAAAGAAGAATAAAAAGATGCTTTCTGTGTTTCTAGTTCCTAAAGTTCCGCTTGTTTATCAGCAAGCAGAAGTTATTCGCGAGCGAACTGGTTTCCAGGTGGGCCATTATTGTGGTGAGATGGGTCAAGATTTTTGGGATACACGAAGATGGCAGCGTGAGTTTGATACTAAACAGGTTCTAGTAATGACAGCTCAAATTCTATTGAACATTCTAAGACACAGCATTATAAAGATGGAATCGATTAATCTATTGATTCTGGATGAGTGCCATCACGCTGTGAAGAAACATCCATACTCTTTAGTGATGTCCGAGTTCTATCATACAACCCCAAAAGAGAAGAGACCATCTGTATTTGGAATGACTGCTTCTCCTGTTAACTTAAAAGGTGTTTCCAATCAACTAGATTGTGCAATAAAGATTCGTAATCTTGAAAGTAAACTGGATTCCGTTGTTTGCACCATAAAGGACCGGAAGGACCTGGAAAAGCATGTTCCAATGCCCTCAGAAATTGTTGTTGAGTATGACAAAGCAGCCAGCTTATGGTCCTTTCACGAACAGCTAAAACAAATGGAAGTGGAAGTTGAAGAAGCTGCAAAGTCTAGCTCTAGAAGAAGTAAATGGCAATTTATGGGAGCTAGAGATGCTGGGGCCAAGGAAGAGCTACGCCAAGTGTATGGTGTTTCAGAAAGAACAGAAAGTGATGGGGCTGTTAACCTAATTCAGAAGCTGAGGGCTATCAATTATGCACTTGGTGAACTGGGTCAGTGGTGCGCCTACAAGGTGGCACAATCATTCTTGACAGCTTTACAAAATGATGAGAGGGCAAACTACCAGCTTGATGTCAAGTTTCAAGAAACTTACCTGATCAGAGTTGTATCTATTTTACAATGTCATCTGTCAGAGGGCGCTGCTTCTGACAAGGAAACAAAACTACCAGATTCAGAGAGTGGCGTTTCTCATGACGAAATTGAGGAAGGAGAGCTACCTGATAGTCATGTTGTTTCTGGTGGGGAGCATGTAGATGTGATAATCGGAGCTGCTGTAGCTGATGGGAAGGTGACGCCAAAAGTGCAGTCACTAATTAAAGTTCTTCTCAAGTATCAGCATACAGAAGATTTCCGTGCAATCATCTTTGTGGAGCGAGTTGTCTCCGCTTTAGTTCTTCCTAAGGTCTTTGCTGAGCTTCCATCTCTAGGTTTTATCGAGTGTGCAAGCTTGATTGGCCACAATAATAGTCAGGAAATGCGAAGTAGCCAAATGCAGGACACAATTGCCAAATTCAAGGATGGTCGTGTGACATTGTTAGTTGCAACTAGTGTTGCTGAGGAAGGACTTGATATTCGACAGTGCAATGTTGTTATTCGCTTTGACCTTGCCAAAACTGTTTTGGCTTATATTCAGTCTAGGGGCCGTGCTAGAAAACCTGGGTCAGATTATATCTTGATGGTGGAAAGGGCAAATCTGTCACATGAAGCATTCTTACGGAATGCTAGAAATAGCGAGGAGACTTTACGGAAAGAAGCAATAGAGAGGACTGACCTGAGTGATTTGAAGGATACTTCAAGGTTAATTTCAGTGGAAACAGCACCAGGTACAGTGTATCAGGTGGAATCAACTGGTGCTTTGGTGAGCTTAAATTCTGCTGTTGGACTAATCCATTTCTACTGCTCTCAGCTTCCGAGTGACAGGTACTCAATACTTCATCCTGAATTTGTTATGGTGAGGCATGAGAAGCAAGAAGGCCCGACTGAATATTCATGCAAGCTTCAACTCCCTTGTAATGCACCATTTGAGACACTTGAAGGCCCCGTGTGCAGTTCAATGCACCTTGCGCAACAGGCTGTTTGTTTGGCTGCTTGTAAGAAACTCCATGAGATGGGAGCATTTACCGACATGCTATTGCCCGACAGGGGAGTTGGGGAAGAAAAAGAAAAGGTTGACAAGAATGACGAAGGAGATCCACTTCCTGGAACTGCTAGGCACAGAGAGTTCTATCCCGAAGGAGTAGCTAATATTCTCCAGGGAGAATGGATCTTAGCTGGAAGAGATCTTGGCAATGAGGCCAAATTGATTCATGTTTACATGTATTCTGTGAAATGTGTAGACATTGGCTCTTCAAAAGATCCTTTCTTGACTCAAGTTTCAGATTTTGCAATTCTGTTAGGCAATGAGCTGGATGCAGAGGTGTTATCGATGTCGATGGATTTATTTGTTGCTCGGACCATGACTACAAAGGCTTCCCTTGTCTTTAGGGGTTCAATTCGTATTACTGAAAGTCAGTTGGCATCCCTTAAAAGTTTCCATGTAAGATTGATGAGTATTGTACTGGATGTGGATGTTGAACCTTCCACCACTCCTTGGGATCCTGCAAAGGCTTATTTATTTGTCCCCGTGGTTAGCGATAAATGTGGAGATCCTATGAAAGAAATAAATTGGAATCTGGTTGAAAATATACTTGGTGCAGATGCGTGGAACAATCCCCTTCAAAGAGCTAGGCCTGATGTTTACCTTGGCACAAATGAGCGAACCCTAGGAGGTGACAGAAGGGAGTATGGTTTTGGTAAACTGCGTCATGGCATGGCTTTTGGGCAAAAATCCCATCCAACCTATGGTATCAGAGGAGCTGTGGCACAGTTTGATGTTGTTAAAGCTTCTGGAGTGATCCCTGATCGGGGTGCCTTTGAAATGCGAAAGGATGTGGATCTGCAGCAACGCAAGTTGATGATGGCAGATAGTTGTACCAATGTTGAAGATTTGGTAGGGAAAATTGTGACAGCTGCTCACTCGGGGAAGAGATTTTATGTTGATTCAATATGCTATGATATGACTGCAGAAAACTCTTTCCCGAGGAAAGAAGGTTATCTTGGCCCTCTGGAGTACAGCTCATATGCTGATTATTACAAGCAAAAGTATGGAGTTGAGTTGATATATAAGAAACAACCTCTAATAAAAGGACGTGGTGTCTCATATTGTAAGAATCTCCTGTCCCCCCGGTTTGACCACATGGAAGAACTTGAAGGTGAATCAGAAGAGTCTCTGGACAAAACATACTATGTATTTCTCCCTCCTGAGCTTTGTTTAGTACACCCACTGTCTGGATCACTTGTCCGTGGAGCCCAAAGATTACCCTCAATAATGAGAAGGGTAGAAAGCATGCTGCTAGCGGTTGAACTTAAGGAGATAATAAATTATCCTGTCCCTGCTTTTAAGATTTTGGAAGCATTGACTGCTGCTTCATGCCAGGAGACATTTTGCTATGAAAGAGCTGAGCTTCTGGGTGATGCCTACCTCAAATGGGTTGTTAGTCGATATCTTTTTCTTAAATACCCTCAGAAACACGAAGGTCAGCTTACTAGGATGAGACAACAAATGGTCAGTAACATGGTTTTGTATCATTATGCATTGAAAAGAGGACTTCAATCATATATCCAAGCAGATCGCTTTGCTCCGTCTCGATGGGCGGCTCCTGGGGTGTTGCCTGTCTTTGATGAGTATACAAAGGATGAAGAATCATCTTTGTTTGACCAAGAGGATGTGACTCGACGGAAAACAGATGACCCGGTTGATGAATATGAGGATGATGAATTGGAGGATGGCGAGCTGGAGAGTGACTTGAGTTCTTATAGGGTCCTGTCAAGCAAGACACTTGCAGATGTTGTTGAAGCGCTTATTGGGGTTTATTATGTAGAAGGTGGGAAAAATGCTGCTAACCATCTCATGAAATGGGTTGGAATTGATGTAGAGTTTAATGCTGATGAGATACAGAGCACAACAAGGCCATCTAATGTTCCAGATAGCATTCTTAGGAGCATCGACTTTGATGCGTTAGAGGGTGCTTTGAATATTAAATTTAGAGACAAGGGCCTCTTGGTGGAAGCCATTAGTCATGCTTCCCGACCATCTTCTGGAGTAGCCTGCTATCAGCGGTTGGAGTTTGTGGGTGATGCTGTCTTAGATCATCTCATCACAAAGCACTTGTTCTTCACATACACAAATCTGCCTCCAGGCAGGTTGACTGATCTTAGAGCTGCTGCTGTTAACAATGAAAATTTTGCACGTGTGGCTGTAAAACATAATCTCCATCTACACCTTCGACATGGATCCAGTGCTCTTGAAAGACAGATTCATGACTTTGTGAAGGAAGCTGCAAATGAATTAACAAAGCCTGGGTTCAACTCATTTGGTTTGGGAGATTGTAAAGCTCCCAAAGTTCTTGGTGACATTATTGAATCTATTGCCGGTGCCATTTTTCTCGATAGTGGACGTGATACTGCAGTTGTTTGGAAGGTGTTTCAACCTCTGCTTCAACCAATGGTCACCCCAGAGACACTACCAATGCATCCGGTCCGTGAGCTGCAAGAAAGATGCCAGCAACAGGCTGAAGGCTTGGAATACAAAGCCAGTCGAAGTGGCAATTTGGCTACTGTTGAAGTCTTGATCGATGGTGTCAAGGTGGGAATTGCTCAGAATCCACAAAAGAAGATGGCACAGAAACTGGCTGCCAGGAATGCTCTTGCTGCTTTAAAAGATAAGGACACAGCTGAAGCAAAGGAGAGGCAGGAGAGGGAGGAGGAAGATAACGGGAAGAAGAAGAAGAATGGTAGCCAAACATTTACCAGGCAAACCTTGAATGACATATGCTTGCGTAAAAACTGGCCTATGCCATTCTACAGGTGTGTCAATGAGGGTGGCCCTGCACATGCAAAGAAGTTTACGTTTGCTGTACGCGTGAATACCACTGACAGGGGATGGATAGATGAATGCGTAGGAGAGCCTATGCCGAGTGTCAAGAAAGCCAAAGATTCGGCAGCAGTTCTTCTATTGGAATTATTGAATAAGTTATACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1956

Amino Acids

219.21

Weight (kDa)

6.0

Isoelectric Point (pI)

41.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	299 - 464	1.7e-14	Type III restriction enzyme, res subunit
DEAD	PF00270	300 - 464	9.1e-16	DEAD/DEAH box helicase
Helicase_C	PF00271	696 - 813	1.6e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	888 - 976	4.4e-22	Dicer dimerisation domain
PAZ	PF02170	1249 - 1389	5e-18	PAZ domain
Ribonucleas_3_3	PF14622	1416 - 1485	1.2e-07	Ribonuclease-III-like
Ribonuclease_3	PF00636	1427 - 1572	2.8e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1626 - 1766	1.5e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1648 - 1761	1.7e-22	Ribonuclease III domain
dsrm	PF00035	1788 - 1848	1e-05	Double-stranded RNA binding motif
DND1_DSRM	PF14709	1878 - 1951	1.7e-15	double strand RNA binding domain from DEAD END PROTEIN 1
dsrm	PF00035	1881 - 1951	2.4e-07	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1247
Acc16I	TGCGCA	1 cut(s)	2853
Acc36I	ACCTGC	1 cut(s)	5019
AccB1I	GGYRCC	3 cut(s)	492, 3695, 5261
AccB7I	CCANNNNNTGG	7 cut(s)	1560, 2896, 2987, 3378, 3629, 4115, 4925
AccBSI	CCGCTC	1 cut(s)	490
AccI	GTMKAC	2 cut(s)	3123, 4514
AccII	CGCG	2 cut(s)	1098, 5748
AcoI	YGGCCR	1 cut(s)	2242
AcsI	RAATTY	9 cut(s)	121, 346, 574, 1215, 2297, 2656, 2729, 4868, 5068
AcuI	CTGAAG	4 cut(s)	876, 884, 5421, 5583
AcvI	CACGTG	1 cut(s)	5078
AcyI	GRCGYC	1 cut(s)	2081
AdeI	CACNNNGTG	2 cut(s)	1297, 1772
AfaI	GTAC	7 cut(s)	2614, 2711, 3345, 3760, 3899, 4104, 5745
AfeI	AGCGCT	1 cut(s)	4686
AflII	CTTAAG	1 cut(s)	4193
AflIII	ACRYGT	3 cut(s)	3102, 5077, 5746
AhdI	GACNNNNNGTC	1 cut(s)	3980
AhlI	ACTAGT	1 cut(s)	2333
AjiI	CACGTC	2 cut(s)	3977, 5285
AjuI	GAANNNNNNNTTGG	6 cut(s)	3459, 3491, 3867, 3899, 4674, 4706
AloI	GAACNNNNNNTCC	2 cut(s)	42, 74
Alw21I	GWGCWC	2 cut(s)	4790, 5131
AlwNI	CAGNNNCTG	2 cut(s)	1747, 5515
Ama87I	CYCGRG	3 cut(s)	603, 3805, 3865
Aor51HI	AGCGCT	1 cut(s)	4686
ApoI	RAATTY	9 cut(s)	121, 346, 574, 1215, 2297, 2656, 2729, 4868, 5068
ArsI	GACNNNNNNTTYG	4 cut(s)	2078, 2110, 2343, 2375
AseI	ATTAAT	1 cut(s)	1263
Asp700I	GAANNNNTTC	2 cut(s)	876, 1092
AspA2I	CCTAGG	1 cut(s)	3550
AspLEI	GCGC	4 cut(s)	1793, 1928, 2854, 4687
AsuC2I	CCSGG	2 cut(s)	4012, 4580
AsuNHI	GCTAGC	1 cut(s)	4180
AvaI	CYCGRG	3 cut(s)	603, 3805, 3865
AvaII	GGWCC	6 cut(s)	1486, 1495, 1563, 3240, 4648, 5365
AvrII	CCTAGG	1 cut(s)	3550
AxyI	CCTNAGG	2 cut(s)	882, 2187
BaeGI	GKGCMC	1 cut(s)	497
BaeI	ACNNNNGTAYC	2 cut(s)	5280, 5313
BalI	TGGCCA	1 cut(s)	2244
BamHI	GGATCC	2 cut(s)	3382, 5120
BanI	GGYRCC	3 cut(s)	492, 3695, 5261
BanII	GRGCYC	1 cut(s)	4138
BarI	GAAGNNNNNNTAC	4 cut(s)	1986, 2018, 2484, 2516
BauI	CACGAG	1 cut(s)	594
BbrPI	CACGTG	1 cut(s)	5078
BbsI	GAAGAC	1 cut(s)	977
Bbv12I	GWGCWC	2 cut(s)	4790, 5131
BbvCI	CCTCAGC	3 cut(s)	563, 1748, 2343
BceAI	ACGGC	1 cut(s)	2415
BcgI	CGANNNNNNTGC	2 cut(s)	4823, 4857
BciVI	GTATCC	2 cut(s)	1150, 2569
BclI	TGATCA	2 cut(s)	199, 1884
BcnI	CCSGG	2 cut(s)	4012, 4580
BcuI	ACTAGT	1 cut(s)	2333
BfmI	CTRYAG	5 cut(s)	2061, 3725, 3849, 5292, 5689
BfoI	RGCGCY	2 cut(s)	1929, 4688
BfrI	CTTAAG	1 cut(s)	4193
BfuAI	ACCTGC	1 cut(s)	5019
BfuI	GTATCC	2 cut(s)	1150, 2569
BglII	AGATCT	1 cut(s)	3067
BlnI	CCTAGG	1 cut(s)	3550
BlpI	GCTNAGC	3 cut(s)	402, 2199, 4287
Bme18I	GGWCC	6 cut(s)	1486, 1495, 1563, 3240, 4648, 5365
BmeRI	GACNNNNNGTC	1 cut(s)	3980
BmeT110I	CYCGRG	3 cut(s)	603, 3805, 3865
BmgBI	CACGTC	2 cut(s)	3977, 5285
BmrI	ACTGGG	2 cut(s)	969, 1787
BmtI	GCTAGC	1 cut(s)	4184
BmuI	ACTGGG	2 cut(s)	969, 1787
BpiI	GAAGAC	1 cut(s)	977
BplI	GAGNNNNNCTC	2 cut(s)	3887, 3919
BpmI	CTGGAG	6 cut(s)	3023, 3696, 3914, 4643, 4947, 5007
Bpu10I	CCTNAGC	4 cut(s)	563, 1748, 2343, 4089
Bpu1102I	GCTNAGC	3 cut(s)	402, 2199, 4287
BpuEI	CTTGAG	3 cut(s)	2099, 3141, 4654
BpuMI	CCSGG	2 cut(s)	4012, 4580
Bsa29I	ATCGAT	3 cut(s)	1259, 3212, 5460
BsaAI	YACGTR	1 cut(s)	5078
BsaBI	GATNNNNATC	2 cut(s)	660, 3720
BsaHI	GRCGYC	1 cut(s)	2081
BsaI	GGTCTC	1 cut(s)	1354
BsaWI	WCCGGW	2 cut(s)	1488, 5362
Bse118I	RCCGGY	2 cut(s)	13, 5258
Bse21I	CCTNAGG	2 cut(s)	882, 2187
Bse3DI	GCAATG	7 cut(s)	49, 280, 978, 1015, 2377, 3082, 3193
Bse8I	GATNNNNATC	2 cut(s)	660, 3720
BseCI	ATCGAT	3 cut(s)	1259, 3212, 5460
BseJI	GATNNNNATC	2 cut(s)	660, 3720
BseMI	GCAATG	7 cut(s)	49, 280, 978, 1015, 2377, 3082, 3193
BseRI	GAGGAG	6 cut(s)	185, 296, 821, 2537, 3654, 5603
BseSI	GKGCMC	1 cut(s)	497
BseYI	CCCAGC	2 cut(s)	965, 1664
BsgI	GTGCAG	4 cut(s)	2111, 2854, 3501, 5700
Bsh1236I	CGCG	2 cut(s)	1098, 5748
BshNI	GGYRCC	3 cut(s)	492, 3695, 5261
BshVI	ATCGAT	3 cut(s)	1259, 3212, 5460
BsiHKAI	GWGCWC	2 cut(s)	4790, 5131
BsiHKCI	CYCGRG	3 cut(s)	603, 3805, 3865
BsiSI	CCGG	7 cut(s)	14, 239, 1489, 4012, 4580, 5259, 5363
BslFI	GGGAC	6 cut(s)	44, 650, 662, 3392, 3991, 4202
BsmBI	CGTCTC	1 cut(s)	4476
BsmFI	GGGAC	6 cut(s)	44, 650, 662, 3392, 3991, 4202
BsmI	GAATGC	5 cut(s)	2495, 2512, 4818, 5533, 5786
Bso31I	GGTCTC	1 cut(s)	1354
BsoBI	CYCGRG	3 cut(s)	603, 3805, 3865
Bsp1286I	GDGCHC	4 cut(s)	497, 4138, 4790, 5131
Bsp1720I	GCTNAGC	3 cut(s)	402, 2199, 4287
Bsp68I	TCGCGA	1 cut(s)	1098
BspDI	ATCGAT	3 cut(s)	1259, 3212, 5460
BspFNI	CGCG	2 cut(s)	1098, 5748
BspHI	TCATGA	3 cut(s)	1978, 4737, 5146
BspMAI	CTGCAG	3 cut(s)	3729, 3853, 5296
BspMI	ACCTGC	1 cut(s)	5019
BspOI	GCTAGC	1 cut(s)	4184
BspQI	GCTCTTC	5 cut(s)	480, 852, 926, 1671, 3139
BspT107I	GGYRCC	3 cut(s)	492, 3695, 5261
BspTI	CTTAAG	1 cut(s)	4193
BspTNI	GGTCTC	1 cut(s)	1354
BsrBI	CCGCTC	1 cut(s)	490
BsrDI	GCAATG	7 cut(s)	49, 280, 978, 1015, 2377, 3082, 3193
BsrFI	RCCGGY	2 cut(s)	13, 5258
BssAI	RCCGGY	2 cut(s)	13, 5258
BssNAI	GTATAC	1 cut(s)	4515
BssNI	GRCGYC	1 cut(s)	2081
BssSI	CACGAG	1 cut(s)	594
BssT1I	CCWWGG	4 cut(s)	1671, 3377, 3529, 3550
Bst1107I	GTATAC	1 cut(s)	4515
Bst2BI	CACGAG	1 cut(s)	594
Bst4CI	ACNGT	9 cut(s)	35, 136, 215, 2367, 2404, 2617, 3654, 4113, 5446
BstACI	GRCGYC	1 cut(s)	2081
BstAFI	CTTAAG	1 cut(s)	4193
BstAPI	GCANNNNNTGC	1 cut(s)	4247
BstBAI	YACGTR	1 cut(s)	5078
BstDSI	CCRYGG	2 cut(s)	3411, 4129
BstEII	GGTNACC	1 cut(s)	5335
BstFNI	CGCG	2 cut(s)	1098, 5748
BstH2I	RGCGCY	2 cut(s)	1929, 4688
BstHHI	GCGC	4 cut(s)	1793, 1928, 2854, 4687
BstNSI	RCATGY	6 cut(s)	1511, 2039, 2917, 3106, 4177, 5723
BstPI	GGTNACC	1 cut(s)	5335
BstSFI	CTRYAG	5 cut(s)	2061, 3725, 3849, 5292, 5689
BstSLI	GKGCMC	1 cut(s)	497
BstUI	CGCG	2 cut(s)	1098, 5748
BstV2I	GAAGAC	1 cut(s)	977
BstX2I	RGATCY	8 cut(s)	2977, 3051, 3067, 3142, 3382, 3433, 3721, 5120
BstXI	CCANNNNNNTGG	2 cut(s)	1130, 1694
BstYI	RGATCY	8 cut(s)	2977, 3051, 3067, 3142, 3382, 3433, 3721, 5120
BstZ17I	GTATAC	1 cut(s)	4515
Bsu15I	ATCGAT	3 cut(s)	1259, 3212, 5460
Bsu36I	CCTNAGG	2 cut(s)	882, 2187
BsuI	GTATCC	2 cut(s)	1150, 2569
BsuTUI	ATCGAT	3 cut(s)	1259, 3212, 5460
BtgI	CCRYGG	2 cut(s)	3411, 4129
BtrI	CACGTC	2 cut(s)	3977, 5285
BtsI	GCAGTG	1 cut(s)	274
BtsIMutI	CAGTG	9 cut(s)	40, 274, 1790, 2372, 2601, 2622, 4109, 5131, 5757
BtuMI	TCGCGA	1 cut(s)	1098
BveI	ACCTGC	1 cut(s)	5019
CaiI	CAGNNNCTG	2 cut(s)	1747, 5515
CciI	TCATGA	3 cut(s)	1978, 4737, 5146
CfoI	GCGC	4 cut(s)	1793, 1928, 2854, 4687
Cfr10I	RCCGGY	2 cut(s)	13, 5258
ClaI	ATCGAT	3 cut(s)	1259, 3212, 5460
CpoI	CGGWCCG	1 cut(s)	5365
CseI	GACGC	2 cut(s)	2089, 3578
CsiI	ACCWGGT	1 cut(s)	2608
Csp6I	GTAC	7 cut(s)	2613, 2710, 3344, 3759, 3898, 4103, 5744
CspCI	CAANNNNNGTGG	2 cut(s)	5689, 5724
CspI	CGGWCCG	1 cut(s)	5365
CviQI	GTAC	7 cut(s)	2613, 2710, 3344, 3759, 3898, 4103, 5744
DraI	TTTAAA	1 cut(s)	5544
DraIII	CACNNNGTG	2 cut(s)	1297, 1772
DriI	GACNNNNNGTC	1 cut(s)	3980
EaeI	YGGCCR	1 cut(s)	2242
Eam1105I	GACNNNNNGTC	1 cut(s)	3980
Eco130I	CCWWGG	4 cut(s)	1671, 3377, 3529, 3550
Eco147I	AGGCCT	2 cut(s)	592, 3517
Eco24I	GRGCYC	1 cut(s)	4138
Eco31I	GGTCTC	1 cut(s)	1354
Eco32I	GATATC	1 cut(s)	4331
Eco47I	GGWCC	6 cut(s)	1486, 1495, 1563, 3240, 4648, 5365
Eco47III	AGCGCT	1 cut(s)	4686
Eco57I	CTGAAG	4 cut(s)	876, 884, 5421, 5583
Eco72I	CACGTG	1 cut(s)	5078
Eco81I	CCTNAGG	2 cut(s)	882, 2187
Eco88I	CYCGRG	3 cut(s)	603, 3805, 3865
Eco91I	GGTNACC	1 cut(s)	5335
EcoO109I	RGGNCCY	4 cut(s)	1495, 2825, 4648, 4882
EcoO65I	GGTNACC	1 cut(s)	5335
EcoRI	GAATTC	1 cut(s)	574
EcoRV	GATATC	1 cut(s)	4331
EcoT14I	CCWWGG	4 cut(s)	1671, 3377, 3529, 3550
EcoT22I	ATGCAT	2 cut(s)	4423, 5361
EcoT38I	GRGCYC	1 cut(s)	4138
ErhI	CCWWGG	4 cut(s)	1671, 3377, 3529, 3550
Esp3I	CGTCTC	1 cut(s)	4476
FalI	AAGNNNNNCTT	8 cut(s)	1053, 1085, 3867, 3899, 4353, 4385, 4871, 4903
FaqI	GGGAC	6 cut(s)	44, 650, 662, 3392, 3991, 4202
FauI	CCCGC	1 cut(s)	185
FauNDI	CATATG	2 cut(s)	3907, 5657
FbaI	TGATCA	2 cut(s)	199, 1884
FblI	GTMKAC	2 cut(s)	3123, 4514
FriOI	GRGCYC	1 cut(s)	4138
FspI	TGCGCA	1 cut(s)	2853
GlaI	GCGC	4 cut(s)	1792, 1927, 2853, 4686
GsaI	CCCAGC	2 cut(s)	969, 1668
GsuI	CTGGAG	6 cut(s)	3023, 3696, 3914, 4643, 4947, 5007
HaeII	RGCGCY	2 cut(s)	1929, 4688
HapII	CCGG	7 cut(s)	14, 239, 1489, 4012, 4580, 5259, 5363
HgaI	GACGC	2 cut(s)	2089, 3578
HhaI	GCGC	4 cut(s)	1793, 1928, 2854, 4687
Hin1I	GRCGYC	1 cut(s)	2081
Hin6I	GCGC	4 cut(s)	1791, 1926, 2852, 4685
HinP1I	GCGC	4 cut(s)	1791, 1926, 2852, 4685
HincII	GTYRAC	5 cut(s)	1396, 1732, 2959, 5034, 5059
HindII	GTYRAC	5 cut(s)	1396, 1732, 2959, 5034, 5059
HindIII	AAGCTT	3 cut(s)	2233, 2783, 3669
HpaI	GTTAAC	3 cut(s)	1396, 1732, 5059
HpaII	CCGG	7 cut(s)	14, 239, 1489, 4012, 4580, 5259, 5363
Hpy99I	CGWCG	1 cut(s)	4566
HpyCH4III	ACNGT	9 cut(s)	35, 136, 215, 2367, 2404, 2617, 3654, 4113, 5446
HpyCH4IV	ACGT	4 cut(s)	3976, 5077, 5284, 5735
HpySE526I	ACGT	4 cut(s)	3976, 5077, 5284, 5735
Hsp92I	GRCGYC	1 cut(s)	2081
HspAI	GCGC	4 cut(s)	1791, 1926, 2852, 4685
KroI	GCCGGC	1 cut(s)	13
KroNI	GCCGGC	1 cut(s)	15
Ksp22I	TGATCA	2 cut(s)	199, 1884
KspAI	GTTAAC	3 cut(s)	1396, 1732, 5059
LguI	GCTCTTC	5 cut(s)	480, 852, 926, 1671, 3139
MabI	ACCWGGT	1 cut(s)	2608
MaeII	ACGT	4 cut(s)	3976, 5077, 5284, 5735
MbiI	CCGCTC	1 cut(s)	490
MfeI	CAATTG	2 cut(s)	905, 2289
MflI	RGATCY	8 cut(s)	2977, 3051, 3067, 3142, 3382, 3433, 3721, 5120
MhlI	GDGCHC	4 cut(s)	497, 4138, 4790, 5131
MlsI	TGGCCA	1 cut(s)	2244
MluI	ACGCGT	1 cut(s)	5746
MluNI	TGGCCA	1 cut(s)	2244
MlyI	GAGTC	3 cut(s)	3148, 4061, 4552
MmeI	TCCRAC	5 cut(s)	563, 2647, 3647, 4729, 4929
Mox20I	TGGCCA	1 cut(s)	2244
Mph1103I	ATGCAT	2 cut(s)	4423, 5361
MroNI	GCCGGC	1 cut(s)	13
MroXI	GAANNNNTTC	2 cut(s)	876, 1092
MscI	TGGCCA	1 cut(s)	2244
MslI	CAYNNNNRTG	4 cut(s)	1128, 1165, 2040, 2153
Msp20I	TGGCCA	1 cut(s)	2244
MspA1I	CMGCKG	4 cut(s)	562, 3797, 4945, 5561
MspCI	CTTAAG	1 cut(s)	4193
MspI	CCGG	7 cut(s)	14, 239, 1489, 4012, 4580, 5259, 5363
MunI	CAATTG	2 cut(s)	905, 2289
Mva1269I	GAATGC	5 cut(s)	2495, 2512, 4818, 5533, 5786
MvnI	CGCG	2 cut(s)	1098, 5748
NaeI	GCCGGC	1 cut(s)	15
NciI	CCSGG	2 cut(s)	4012, 4580
NdeI	CATATG	2 cut(s)	3907, 5657
NgoMIV	GCCGGC	1 cut(s)	13
NheI	GCTAGC	1 cut(s)	4180
NmeAIII	GCCGAG	1 cut(s)	5826
NruI	TCGCGA	1 cut(s)	1098
NsbI	TGCGCA	1 cut(s)	2853
NsiI	ATGCAT	2 cut(s)	4423, 5361
NspI	RCATGY	6 cut(s)	1511, 2039, 2917, 3106, 4177, 5723
PaeI	GCATGC	1 cut(s)	4177
PagI	TCATGA	3 cut(s)	1978, 4737, 5146
PceI	AGGCCT	2 cut(s)	592, 3517
PciI	ACATGT	1 cut(s)	3102
PciSI	GCTCTTC	5 cut(s)	480, 852, 926, 1671, 3139
PctI	GAATGC	5 cut(s)	2495, 2512, 4818, 5533, 5786
PdiI	GCCGGC	1 cut(s)	15
PdmI	GAANNNNTTC	2 cut(s)	876, 1092
PflMI	CCANNNNNTGG	7 cut(s)	1560, 2896, 2987, 3378, 3629, 4115, 4925
PfoI	TCCNGGA	1 cut(s)	2986
PleI	GAGTC	3 cut(s)	3148, 4060, 4552
PmaCI	CACGTG	1 cut(s)	5078
PmlI	CACGTG	1 cut(s)	5078
PpsI	GAGTC	3 cut(s)	3148, 4060, 4552
Ppu21I	YACGTR	1 cut(s)	5078
PpuMI	RGGWCCY	2 cut(s)	1495, 4648
PscI	ACATGT	1 cut(s)	3102
PshBI	ATTAAT	1 cut(s)	1263
PsiI	TTATAA	1 cut(s)	1247
Psp5II	RGGWCCY	2 cut(s)	1495, 4648
PspCI	CACGTG	1 cut(s)	5078
PspEI	GGTNACC	1 cut(s)	5335
PspFI	CCCAGC	2 cut(s)	965, 1664
PspPPI	RGGWCCY	2 cut(s)	1495, 4648
PstI	CTGCAG	3 cut(s)	3729, 3853, 5296
PstNI	CAGNNNCTG	2 cut(s)	1747, 5515
PsuI	RGATCY	8 cut(s)	2977, 3051, 3067, 3142, 3382, 3433, 3721, 5120
PvuII	CAGCTG	3 cut(s)	562, 3797, 5561
RruI	TCGCGA	1 cut(s)	1098
RsaI	GTAC	7 cut(s)	2614, 2711, 3345, 3760, 3899, 4104, 5745
RsaNI	GTAC	7 cut(s)	2613, 2710, 3344, 3759, 3898, 4103, 5744
RseI	CAYNNNNRTG	4 cut(s)	1128, 1165, 2040, 2153
Rsr2I	CGGWCCG	1 cut(s)	5365
RsrII	CGGWCCG	1 cut(s)	5365
SapI	GCTCTTC	5 cut(s)	480, 852, 926, 1671, 3139
SchI	GAGTC	3 cut(s)	3148, 4061, 4552
SduI	GDGCHC	4 cut(s)	497, 4138, 4790, 5131
SexAI	ACCWGGT	1 cut(s)	2608
SfcI	CTRYAG	5 cut(s)	2061, 3725, 3849, 5292, 5689
SinI	GGWCC	6 cut(s)	1486, 1495, 1563, 3240, 4648, 5365
SmiMI	CAYNNNNRTG	4 cut(s)	1128, 1165, 2040, 2153
SmlI	CTYRAG	4 cut(s)	2114, 3156, 4193, 4633
SmoI	CTYRAG	4 cut(s)	2114, 3156, 4193, 4633
SpeI	ACTAGT	1 cut(s)	2333
SphI	GCATGC	1 cut(s)	4177
SseBI	AGGCCT	2 cut(s)	592, 3517
SspI	AATATT	3 cut(s)	2774, 3034, 4864
StuI	AGGCCT	2 cut(s)	592, 3517
StyI	CCWWGG	4 cut(s)	1671, 3377, 3529, 3550
TaaI	ACNGT	9 cut(s)	35, 136, 215, 2367, 2404, 2617, 3654, 4113, 5446
TaiI	ACGT	4 cut(s)	3979, 5080, 5287, 5738
TatI	WGTACW	3 cut(s)	3343, 3897, 4102
TauI	GCSGC	1 cut(s)	4484
TscAI	CASTG	9 cut(s)	40, 274, 1790, 2372, 2601, 2622, 4116, 5131, 5764
TspGWI	ACGGA	8 cut(s)	1456, 2129, 2518, 2548, 4118, 4458, 4579, 5357
TspRI	CASTG	9 cut(s)	40, 274, 1790, 2372, 2601, 2622, 4116, 5131, 5764
Van91I	CCANNNNNTGG	7 cut(s)	1560, 2896, 2987, 3378, 3629, 4115, 4925
Vha464I	CTTAAG	1 cut(s)	4193
VpaK11BI	GGWCC	6 cut(s)	1486, 1495, 1563, 3240, 4648, 5365
VspI	ATTAAT	1 cut(s)	1263
XapI	RAATTY	9 cut(s)	121, 346, 574, 1215, 2297, 2656, 2729, 4868, 5068
XbaI	TCTAGA	1 cut(s)	1625
XceI	RCATGY	6 cut(s)	1511, 2039, 2917, 3106, 4177, 5723
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2404, 3626
XmaJI	CCTAGG	1 cut(s)	3550
XmiI	GTMKAC	2 cut(s)	3123, 4514
XmnI	GAANNNNTTC	2 cut(s)	876, 1092
Zsp2I	ATGCAT	2 cut(s)	4423, 5361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.