AT1G02080

Domain of unknown function (DUF3819)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005282
Physical Location & Seq
Forward (+)
373335 .. 386913
13579 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G02080.1

Sequence Viewer

Length: 7296 bp
ATGTATTCAAAGACAATTGAAAATCCAGACGAGACGAGTCCACGAATAATGTGGGCCTTAAAAAGAGTCAGCTACTCTCGTGGATCCACCGAAAACCCTAGAAGGAAGAGCCCCTCTCCCGTCGTCCCTGGCATTTCGGATCTCGGATTTCCTTACCTCATGACTCCCTCCAAGGTCGCCGGTCATACCCGGTTCTTGCTCCACAGCTTTCATGATTCCGATGTCGACTCCATCGCCCTCCAGCTTTCTCAGTTTGTTGATTTTGGAGTCGAGACTAGTATTCCAGTTCTCAAGACCTGCCTGGATTGCTTCACCGCCCGGAGAAGCCATCCTAATTCGTTGCAGCTTGAGAAAGTTGTATCCTTGCTCTTTAAACATGTGCTCAAGTTGTCAAACCTTGCCACCCTGCTTCCTCACGCCCTCAATGATTTTGAGCTCACTCAAGAATCCGTGGATGATTTGACTACTACTTTGAATTTTTCAATCTCTGAGAATATTGGCTTTGCTCTCGCCCTGACCGATTTTGAAAGATTAGATGCAAAGACCACTGGACGGAACTTACTGTTGGCTCAGATTGAGCAACTCTGTGCCAATACTGGCCAGATTCTTTCATCTGAGCTAATCCATTCTGTTCTTTCGTTTCTCCGCAAATCTGAGGATCTTTCCATGCATTTGGATTCCTTCTTACAGTTTTTGTCTTCTGCTCAACCTAGAGATGACTTCTCTTTTGCTCTAACACCTATGCTTGCTCAACAAGTTCATGAAGCTCCTGTTTTCAGGAGCATGGATTTTCACACTGATTCTGCTGATAACGACCTTGATGCTATCTTAGCTGAAATCGATAAGGAAGTCAGCGTTGGAGATCTCATGGGAGAATTAGGTTGTGGATTCACTGCTGATGCCCAACAATGCAAAGAAATCTTGTCTAGTTTTGCTCCACTAGGAGAGGCTACTATCTCAAGAATTGTTGGCAATGTTTCTCGGACCTGTGCTGATCTTGAAGATAATCAAACCACCTTTTCAACGTTTACTGTAGCCCTTGGTTCCTGCATTCCAACTGAACTTCCCACGCCAAGGTCGTGGAATGTTGATATTCTAGTTGACACAATCAAGCAGCTTCAGGCTCCTGGCATAAGCTGGAGGAAAGTAATTGAGAATCTCGATCATGACGGATTTGACATCCCTAATATGGAGTCTTTCTCATTTTTTATGCGGATCTATAAAGCTGCTTGCAAGGAGCCATTTCCTCTTGATGCTGTATGTGGTTCTGTCTGGAAAAATATGGATGGTCAATTATCCTTTCTTAAGCACGCAATATCTGCTCCACCAGAAGTATTCACCTTTATGCATTCTCCAAGGAAGCTGGTATATATTGATAACATGCACAGCCAAGAGCAACAGTTAGGACTATCCAATCATGCATGGCTGTCGCTCGATCTCTTGGATGTACTGTGCCAATTGGCCGAGAGAGGTCATGCTGTTTTAGTTAGTTCGTTACTCCAGTATCCACTCACACAGTGTCCTAGAACCTTGCTTCTTGGAATGACACACATCAAAACTGCCTATAACCTCATACAGCGTGAAGTGGTTTCTGCTATTCTGCCAGTGATAATAACGAGTCCCCAGGATAGTGGTTTTATCCATAATCTCTGGCATCAAAATGCAGAACTCGTTCTGTGGGGGATTATAGATGCCCAACATCTCAAAGCAGACAGCATGCTCCGAATAATTGAAATCTGCCATGAGCTAAAGATTCTCTCTGTTGTCTTAGAATCAGTTCCAGTCTCATCCAGTATCAGATTGGCAGTCCTTGCCTCATTGAGAGGTCTTTTGGACATTGAGAACTGGTTGCCTAACTGCTTGTATATGTATAAGGATCTCTTTGCTGAGGAGTGCCTCAAATTTGTCAAAAATGTGCATTTTAGCGAATCAGACGATTTCAGGGCCAAAATTTTTCATCCTTCTGATCCATTATCAGATCTTCATTTGGAAGCAACAACTTCCCTTTTAAAAGTTTTGAAAGCTCATGATAATGCGATTACTTCATCTCAACTGGTCGAGGAGATTGAAAAAGTCAATGCAGCTATTTTGGATTGTAATCCTAAGCTGCAGAATGGTGAGGCTAAAGATTCATCAGCTCCCAATGCATATGGAGATGATGTTGAGGCAGAGGCAAACGCATATTTTCATCAAATGTTTTCTAGTCATTTGAGTGTTGACGCAATGGTTCAAATGCTTTCCCGATACAAGGAATCTTTGGTTCCAAGGGAAAAATTAATTTTTGAATGCATGATTGCTAATCTGTTTGAAGAATATCGGTTTTTCCCCAAGTATCCTGAGAGACAGCTGAAAATAGCCTCCATACTCTTTGGTTCTGTTATAAAACACCAGCTTATAAGTTCGCTCACTCTGGGGATGGCCCTGCGTCTAGTGTTGGATTCTTTGCGTAAACCTGCAGATTCAAAAATGTTTCTGTTTGGAAGCAAAGCTCTGGAACAGTTTGTGAATCGTCTGGTTGAATTGCCTCAATACTGTAACCATATTTTGCAAATATCTCATCTGCGTAGTACTCACCCGGAGTTAGTCACTGTTATTGAACAAGCACTTTCAAGGATATCATCTGGTAATTTAGAATCAGATGCCTCTGTTTCACACCCTGGTCCCTCACAATCTTTCCCTGGAAATGGCGAGTTGAGTGGTTCTGGAATTGGCCAACCTGCATTACAGCTTTCTTCTCCCCTACAGCTTCAACAGAAAAATGAAGTGCCAAGTGTGCCATCTAACGAGGCAAAGCCACTTCTGCCATCGTTATCAACTACTTCCGTTGATGTTTCTGTCAATCCTAAGAATCCTGGCATTCCAACTTCTTCATCAACCTCAACTGGTTTTGTTCGTCCAGCTCGTGCAACCACTTCAACAAGGTTTGGCTCGGCTTTGAACATAGAAACCTTGGTTGCAGCAGCAGAAAGAAGGGAAAACGCAATCGAGGCTCCACCTTCAGATGTTCAGGACAAAGTTTCGTTCATAATCAATAATATCTCTACAACAAACATTGAATCTAAAGGGAAAGAGTTTGCTGAAATTTTGCCCCAACAGTATTATCCTTGGTTTGCACAGTACATGGTTATGAAAAGAGCGAGCATCGAGCCGAATTTTCATGATCTGTACCTGAAGTTCTTGGACAAAGTTGATTCCAAGCTGCTGTTTAAGGAGATACTTCAAAATACTTACGAAAATTGCAAGGTTCTTTTGGGCTCAGAGCTCATAAAGTCGAGTTCTGAAGAGCGTTCTCTTCTTAAAAATTTAGGCAGTTGGCTTGGAAGGTTGACCATTGGAAGGAATTATGTTTTAAGGGCGCGAGAAATAGATCCAAAATCCTTGATTGTGGAGGCCTATGAAAAAGGGTTGATGATAGCAGTCATTCCGTTCACTTCAAAGGTTCTGGAGCCATGCCAAAACAGTATTGCTTATCAACCTCCCAATCCTTGGACGATGGCTATACTAGGGTTGCTTGCTGAGATCTATTCAATGCCAAACCTAAAAATGAATCTGAAGTTTGACATAGAGGTTTTATTCAAGAACCTTGGTGTTGAAATGAAGGAAGTAGTGCCAACTTCCCTTCTAAAGGATCGGAAGAGAGAAATAGATGGCAATCCTGATTTTAGTAACAAAGATCCTGGAGTAACACAGATATCTCAGCCACAGATGATTCCAGAGCCTAAAACAATTTCTCCTCTTAAACAAATTGACCTACCTCTTGATGTTGCAAATTCGCCTAATACAGATGTTCCCTCGAAGTTATTATCACAGTATGTAGCCCCACAACGTGTTTATACTAATACTTTGATGGATGAGGAGAAAGTAGCAACTCTTGGCTTGCCAGAACAGCTCCCGTCACCTCAAGGACTGTTCCAGTCAACTCCATCGCCATTGTTTTCTATTAGTCAGCTGTCAGCAGCACTTCCCAATATTGGAAATCATGTTGTTATCAATCAGAAATTAAGCGCATTTGGTATGCACTTTCCATTTCAAAGAGTGGTACCACTTGCCATGGACAGAGCTATCAAGGAGATTGTGTCTGGTATTGTTCAGCGAAGTGTTTGTATTGCATGCCAAACAACAAAGGAGCTTGTGCTAAAGGATTACGCCCTGGAACCCGATGAGTCACGTATTTATAATGCAGCTCACTTGATGGTTGCGAGTTTAGCTGGGAGTTTGGCTCATGTGACTTGCAAGGAACCCCTGCGCACTTCAATATCCGGTCATCTACGGAATTCGCTTCAGGGTCTGAATATTTCAAATGATGCTCTTGAACAAATTGTGCAACTTGTCACCAATGACAACCTGGATTTGGGTTGTGCTGCTATTGAACAGGCTGCTACAGAAAAGGCAATACAAACAATTGATGCGGACATTGCTCAACAATTGTTGTTACGGAGAAAGCATAGAGATGGTGCTGGATCCTCCTTTTTCGATCCAAACATTCTATCACAGAATTCCGTCAGTTTTATACCCGAATCCCTCCGTCCAAAACCTGGACACCTCTCCCTGTCTCAGCAGAGAGTTTATGAGGACTTTGTTCAACATCCTTGGCAAAAGCAGTCGACCCAAACTTCACACGGTTTATCTGCTGCATCAAGCTCATCTGGTGATGTTGCGCTTGGTAGTGGTTATGGTCCAGTATCAGGAAAAGTTGCTTCTGAATTTTTGTCTAATGCTGGAAACGCTCGGATGGACATGGTCTCCCGGCCATCAGATATTTCTGTGGATGGTTTTGAATCTTCTCCAGTCTCACTTTTGAGCTCACAAGTCGATCCAGCTGGTGACTCATCCAGTCTTCAATTTACTAAGTCACTACCAACCTCTGAATTGAATCTGGCTGAATCCTCTGACGCTGCTACGAAAGAAACTGGAACATCACTGCAGACATTGACTTCAGCTGCTACCATGGAACGACTTGGTGCTAGTAATATTACACAGCCTTCACTGTCCACGAGAGATGCACTGGATAAATGTCAAATTGTTACTCAGAAGATGGAGGAATTAGTGGCTAATAATGCAGGAGATGATGAAATCCAGGCCGTAATTTCTGAAGTTCCTGAAATCATCCTCAGATGTATAAGCAGAGATGAAGCCGCTTTTGCTGTCGCTCAAAAAGCTTTCAAGGCTCTTTACGAGAATGCATCAAGTAACCTTCACGTCAGTGCTAACCTAGCAATACTTGTTGCTATCCGTGATGTTTGCAAGCGTGTTGTTAAAGAGCTCACTAGTTGGGTGATTTATTCAGAGGAGGATCGGAAGCTCAACAAAGATATTACTATTGGTCTTATCCAGCGTGAGTTGCTTAGCCTAGCGGAGTACAATGTCCACATGGCGAAGCATCTTGATGGAGGGAGAAACAAGACCGCAACTGACTTTGCTATTTCTCTACTCCAATCCTTGGTCACTGAGGAGTCGAGTGTCATTTCAGAGCTCCACAGTCTTGTTGATGCGCTGGCAAAGCTTGCCTCAAAATCCGGATCTTCTGAGTCATTGCAACAGCTAATTGACATCATACGAAATCCAGTTACTAACACAGCTGGCCTCTCTGATAGTTCAACCGGAAATGATAACAACGATAGGCAAAAGGATGAGAAGGTTGCGTGCAACACCACTAACACAGAAGAGAGTACCAGCTTGGATTATGTGGAATCAGATCCTGCGGGTTTCCAGAATCGGGTGTCCACGCTATTTAAAAACTGGTATCAAATCTGCGAACTTCCTGGTGCAAATGAAACCGCATGTTCACAATATGTCCTGCATTTGCACCAGACTGGATTACTTAAGGGAGATGATACAACAGAGAGTTTTTTCCGAATTCTTCTGGAACTTTCTGTTGCTCATTGTATATCTTCTGAAGATATTAATTCCGGTGCTGTGCAATCTCCTCAGCAACCTCAGAGTCCATCATTCCTTATCATTGATATGTATGCAAAGCTTGTTTTTTCAATATTGAAGTATTTCCCTGAGCAGGAGTCTAGCAGTAGGTTATTTCTTCTTTCAGAGATCATGGCTGACACAGTGAGATTTATTCAAAAGGACGCAGAAGATAAAAAGACATCACTCAATTCTAAACCATATTTCCGATTGTTCATCAACTGGCTACTGGACTTGTGTTCCCTGGATCCTGGGACGGATGGTGCAAACTTTCAGGTTCTCACAGCTTTTGCCAACGCCTTCCACGCATTGCAGCCTCTTAAGATTCCTGCTTTCAGTTTTGCATGGCTAGAACTTGTCAGCCACAGAAGCTTCATGCCCAAACTACTTACAGTAAATGGCCAGAAGGGTTGGCCATATGTTCAGCGCCTGCTGGTGGACTTGCTTCAGTTTCTTGAGCCATTTTTAAGAAATGCTGAACTCGGAGGACCGGTTCATTTCCTATACAAAGGGACATTGAGAGTATTACTGGTTCTGCTTCATGATTTCCCAGAGTTCCTATGTGATTATCATTTTACTTTCTGTGATGTGATTCCTTCAAGTTGCATACAAATGCGGAATATTATCCTCAGTTCTTTTCCACGGAACATGAGGCTTCCTGATCCTTCTACCCCAAATTTAAAGATTGATTTGTTACCTGAGATAGTAGAAGCTCCCTGTATCCTTTCTGAGGTTGATGCTGCGCTAAAGGCAAAGCAAATGAAGAATGACGTGGATGAGTATCTTACGTCGAGGCAGCAAAATTCAACTTTCCTAAGTGAGTTGAAGACAAAGTTACTTCTATCGTCCAGCGAGGCTAGTTCAGCTGGAACCCGTTACAGTGTACCATTGATCAACTCGCTTGTGCTATATACTGGAATGCAGGCTATTCAGCAGCTTCAGGCCGGTGAGACACAGGCTCAAAATGTTGTGGCCTTGCAGATGTTCAAGTATTTAAGTATGGAACTTGATACGGAAGGGCGGTATCTGTTCCTTAATGCAATCGCCAATCAGCTTCGATACCCCAACAACCACACTCATTACTTTTCTTTCATCATGCTCTATCTATTCTTCGAATCTGATCAGGAGATCATACAGGAGCAAATAACTAGAGTGCTGTTGGAACGGCTAATTGTGAACAGGCCACATCCATGGGGACTTCTAATCACATTCATTGAGCTAATAAAGAACCCAAGATATAGCTTCTGGAAACAAGCCTTCATCAGATGTGCGCCTGAGATTGAGAAGCTGTTTGAATCGGTAGCCAGATCCTGTGGTGGCCTGAAGCCTGTCGACGAGGGAATGGTTTCGGGAGGTTGGGTCTCAGACAATTCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2431

Amino Acids

269.88

Weight (kDa)

5.39

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 528 - 674 1.1e-27 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 702 - 871 5e-63 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 995 - 1214 4.1e-89 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1343 - 1483 9.3e-52 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
ARM_Cnot1 PF25097 1677 - 1854 7e-50 Cnot1 ARM repeat
NOT1_connector PF23590 1677 - 1853 4.5e-13 CCR4-NOT transcription complex subunit 1, NOT1_connector
Not1 PF04054 2058 - 2407 1.2e-141 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2381, 2396, 4176
Acc16I TGCGCA 1 cut(s) 4247
Acc36I ACCTGC 3 cut(s) 305, 2461, 2725
Acc65I GGTACC 1 cut(s) 4039
AccB1I GGYRCC 1 cut(s) 4039
AccB7I CCANNNNNTGG 3 cut(s) 3486, 4535, 5440
AccI GTMKAC 3 cut(s) 225, 4604, 7248
AccII CGCG 1 cut(s) 3358
AccIII TCCGGA 1 cut(s) 5516
AclI AACGTT 1 cut(s) 1025
AcoI YGGCCR 6 cut(s) 598, 1461, 2710, 4748, 6316, 6329
AdeI CACNNNGTG 2 cut(s) 1518, 3827
AfaI GTAC 8 cut(s) 1449, 2569, 3119, 3167, 4041, 5360, 5671, 6801
AflII CTTAAG 3 cut(s) 1304, 5822, 6236
AflIII ACRYGT 2 cut(s) 376, 3826
AgeI ACCGGT 1 cut(s) 6406
AhlI ACTAGT 2 cut(s) 275, 5267
AjiI CACGTC 2 cut(s) 5200, 6688
AjuI GAANNNNNNNTTGG 4 cut(s) 548, 580, 840, 872
AleI CACNNNNGTG 1 cut(s) 5202
AloI GAACNNNNNNTCC 2 cut(s) 6393, 6425
Alw21I GWGCWC 6 cut(s) 384, 438, 3264, 4805, 5265, 5475
Alw26I GTCTC 9 cut(s) 26, 266, 1789, 2335, 4557, 4747, 4795, 6860, 7282
AlwNI CAGNNNCTG 4 cut(s) 4288, 5699, 6346, 7227
Aor13HI TCCGGA 1 cut(s) 5516
ArsI GACNNNNNNTTYG 4 cut(s) 454, 486, 710, 742
AseI ATTAAT 2 cut(s) 2276, 5904
AsiGI ACCGGT 1 cut(s) 6406
Asp700I GAANNNNTTC 4 cut(s) 1671, 1776, 5999, 7261
Asp718I GGTACC 1 cut(s) 4039
AspLEI GCGC 8 cut(s) 3358, 4007, 4248, 4660, 5494, 6345, 6661, 7189
AspS9I GGNCC 7 cut(s) 54, 984, 1944, 2419, 2660, 4676, 6404
AsuC2I CCSGG 4 cut(s) 190, 319, 2576, 4747
AsuII TTCGAA 1 cut(s) 7029
AvaII GGWCC 4 cut(s) 984, 2660, 4676, 6404
BaeI ACNNNNGTAYC 2 cut(s) 1487, 1520
BalI TGGCCA 4 cut(s) 600, 2712, 6318, 6331
BamHI GGATCC 3 cut(s) 83, 4460, 6163
BanI GGYRCC 1 cut(s) 4039
BanII GRGCYC 7 cut(s) 113, 438, 3257, 3264, 4805, 5265, 5475
BauI CACGAG 3 cut(s) 78, 2901, 4993
BbsI GAAGAC 3 cut(s) 690, 4829, 6749
Bbv12I GWGCWC 6 cut(s) 384, 438, 3264, 4805, 5265, 5475
BbvCI CCTCAGC 2 cut(s) 1887, 5928
BceAI ACGGC 2 cut(s) 5066, 7097
BcgI CGANNNNNNTGC 4 cut(s) 803, 837, 1410, 1444
BciVI GTATCC 4 cut(s) 370, 1515, 2343, 6647
BclI TGATCA 2 cut(s) 6807, 7036
BcnI CCSGG 4 cut(s) 190, 319, 2576, 4747
BcoDI GTCTC 9 cut(s) 26, 266, 1789, 2335, 4557, 4747, 4795, 6860, 7282
BcuI ACTAGT 2 cut(s) 275, 5267
BfmI CTRYAG 6 cut(s) 1032, 2108, 2454, 2741, 4380, 4922
BfoI RGCGCY 1 cut(s) 6346
BfrI CTTAAG 3 cut(s) 1304, 5822, 6236
BfuAI ACCTGC 3 cut(s) 305, 2461, 2725
BfuI GTATCC 4 cut(s) 370, 1515, 2343, 6647
BglII AGATCT 3 cut(s) 862, 1978, 3519
BlpI GCTNAGC 1 cut(s) 5345
BmcAI AGTACT 1 cut(s) 2569
Bme18I GGWCC 4 cut(s) 984, 2660, 4676, 6404
BmgBI CACGTC 2 cut(s) 5200, 6688
BmgT120I GGNCC 7 cut(s) 54, 984, 1944, 2419, 2660, 4676, 6404
BpiI GAAGAC 3 cut(s) 690, 4829, 6749
BplI GAGNNNNNCTC 6 cut(s) 100, 132, 1184, 1216, 4204, 4236
BpmI CTGGAG 6 cut(s) 224, 1159, 1484, 3464, 3699, 4770
Bpu10I CCTNAGC 4 cut(s) 1887, 2103, 5928, 6006
Bpu1102I GCTNAGC 1 cut(s) 5345
Bpu14I TTCGAA 1 cut(s) 7029
BpuEI CTTGAG 7 cut(s) 275, 368, 368, 426, 943, 3885, 6392
BpuMI CCSGG 4 cut(s) 190, 319, 2576, 4747
Bsa29I ATCGAT 1 cut(s) 840
BsaAI YACGTR 1 cut(s) 4169
BsaBI GATNNNNATC 3 cut(s) 2097, 3651, 6696
BsaI GGTCTC 2 cut(s) 4747, 7282
BsaWI WCCGGW 5 cut(s) 4259, 5516, 5600, 5909, 6406
Bse118I RCCGGY 3 cut(s) 179, 6406, 6860
Bse3DI GCAATG 5 cut(s) 979, 2229, 4413, 5531, 6224
Bse8I GATNNNNATC 3 cut(s) 2097, 3651, 6696
BseAI TCCGGA 1 cut(s) 5516
BseCI ATCGAT 1 cut(s) 840
BseJI GATNNNNATC 3 cut(s) 2097, 3651, 6696
BseMI GCAATG 5 cut(s) 979, 2229, 4413, 5531, 6224
BseRI GAGGAG 7 cut(s) 1904, 2075, 3723, 3869, 5303, 5465, 5916
BseYI CCCAGC 1 cut(s) 4208
Bsh1236I CGCG 1 cut(s) 3358
BshNI GGYRCC 1 cut(s) 4039
BshTI ACCGGT 1 cut(s) 6406
BshVI ATCGAT 1 cut(s) 840
BsiHKAI GWGCWC 6 cut(s) 384, 438, 3264, 4805, 5265, 5475
BslFI GGGAC 6 cut(s) 110, 1605, 2646, 6184, 6442, 7125
BsmAI GTCTC 9 cut(s) 26, 266, 1789, 2335, 4557, 4747, 4795, 6860, 7282
BsmBI CGTCTC 1 cut(s) 26
BsmFI GGGAC 6 cut(s) 110, 1605, 2646, 6184, 6442, 7125
BsmI GAATGC 6 cut(s) 1050, 1348, 2291, 2856, 5185, 6840
Bso31I GGTCTC 2 cut(s) 4747, 7282
Bsp119I TTCGAA 1 cut(s) 7029
Bsp1286I GDGCHC 8 cut(s) 113, 384, 438, 3257, 3264, 4805, 5265, 5475
Bsp13I TCCGGA 1 cut(s) 5516
Bsp1720I GCTNAGC 1 cut(s) 5345
Bsp19I CCATGG 3 cut(s) 4050, 4947, 7106
BspDI ATCGAT 1 cut(s) 840
BspEI TCCGGA 1 cut(s) 5516
BspFNI CGCG 1 cut(s) 3358
BspHI TCATGA 7 cut(s) 159, 211, 760, 1165, 2026, 3157, 6457
BspMAI CTGCAG 3 cut(s) 2112, 2458, 4926
BspMI ACCTGC 3 cut(s) 305, 2461, 2725
BspQI GCTCTTC 2 cut(s) 101, 3276
BspT104I TTCGAA 1 cut(s) 7029
BspT107I GGYRCC 1 cut(s) 4039
BspTI CTTAAG 3 cut(s) 1304, 5822, 6236
BspTNI GGTCTC 2 cut(s) 4747, 7282
BsrDI GCAATG 5 cut(s) 979, 2229, 4413, 5531, 6224
BsrFI RCCGGY 3 cut(s) 179, 6406, 6860
BssAI RCCGGY 3 cut(s) 179, 6406, 6860
BssSI CACGAG 3 cut(s) 78, 2901, 4993
Bst2BI CACGAG 3 cut(s) 78, 2901, 4993
Bst6I CTCTTC 5 cut(s) 101, 3276, 3297, 3629, 5658
BstAFI CTTAAG 3 cut(s) 1304, 5822, 6236
BstAPI GCANNNNNTGC 3 cut(s) 1319, 1811, 5504
BstBAI YACGTR 1 cut(s) 4169
BstBI TTCGAA 1 cut(s) 7029
BstDSI CCRYGG 5 cut(s) 450, 4050, 4947, 6557, 7106
BstENI CCTNNNNNAGG 2 cut(s) 3623, 6644
BstFNI CGCG 1 cut(s) 3358
BstH2I RGCGCY 1 cut(s) 6346
BstHHI GCGC 8 cut(s) 3358, 4007, 4248, 4660, 5494, 6345, 6661, 7189
BstMAI GTCTC 9 cut(s) 26, 266, 1789, 2335, 4557, 4747, 4795, 6860, 7282
BstNSI RCATGY 5 cut(s) 380, 1384, 1720, 4113, 5784
BstSFI CTRYAG 6 cut(s) 1032, 2108, 2454, 2741, 4380, 4922
BstUI CGCG 1 cut(s) 3358
BstV2I GAAGAC 3 cut(s) 690, 4829, 6749
BstXI CCANNNNNNTGG 4 cut(s) 673, 1080, 1631, 7107
Bsu15I ATCGAT 1 cut(s) 840
BsuI GTATCC 4 cut(s) 370, 1515, 2343, 6647
BsuTUI ATCGAT 1 cut(s) 840
BtgI CCRYGG 5 cut(s) 450, 4050, 4947, 6557, 7106
BtgZI GCGATG 2 cut(s) 217, 3909
BtrI CACGTC 2 cut(s) 5200, 6688
BtsI GCAGTG 2 cut(s) 891, 4919
BveI ACCTGC 3 cut(s) 305, 2461, 2725
CaiI CAGNNNCTG 4 cut(s) 4288, 5699, 6346, 7227
CciI TCATGA 7 cut(s) 159, 211, 760, 1165, 2026, 3157, 6457
CfoI GCGC 8 cut(s) 3358, 4007, 4248, 4660, 5494, 6345, 6661, 7189
Cfr10I RCCGGY 3 cut(s) 179, 6406, 6860
Cfr13I GGNCC 7 cut(s) 54, 984, 1944, 2419, 2660, 4676, 6404
ClaI ATCGAT 1 cut(s) 840
CseI GACGC 4 cut(s) 2228, 2414, 4901, 6089
Csp6I GTAC 8 cut(s) 1448, 2568, 3118, 3166, 4040, 5359, 5670, 6800
CspAI ACCGGT 1 cut(s) 6406
CviQI GTAC 8 cut(s) 1448, 2568, 3118, 3166, 4040, 5359, 5670, 6800
DraI TTTAAA 4 cut(s) 373, 2010, 5734, 6597
DraIII CACNNNGTG 2 cut(s) 1518, 3827
EaeI YGGCCR 6 cut(s) 598, 1461, 2710, 4748, 6316, 6329
Eam1104I CTCTTC 5 cut(s) 101, 3276, 3297, 3629, 5658
EarI CTCTTC 5 cut(s) 101, 3276, 3297, 3629, 5658
Ecl136II GAGCTC 5 cut(s) 436, 3262, 4803, 5263, 5473
Eco147I AGGCCT 1 cut(s) 3392
Eco24I GRGCYC 7 cut(s) 113, 438, 3257, 3264, 4805, 5265, 5475
Eco31I GGTCTC 2 cut(s) 4747, 7282
Eco32I GATATC 2 cut(s) 2616, 3693
Eco47I GGWCC 4 cut(s) 984, 2660, 4676, 6404
Eco53kI GAGCTC 5 cut(s) 436, 3262, 4803, 5263, 5473
EcoICRI GAGCTC 5 cut(s) 436, 3262, 4803, 5263, 5473
EcoNI CCTNNNNNAGG 2 cut(s) 3623, 6644
EcoRI GAATTC 3 cut(s) 4273, 4495, 5856
EcoRV GATATC 2 cut(s) 2616, 3693
EcoT22I ATGCAT 6 cut(s) 672, 1350, 1423, 2149, 2291, 5185
EcoT38I GRGCYC 7 cut(s) 113, 438, 3257, 3264, 4805, 5265, 5475
Esp3I CGTCTC 1 cut(s) 26
FalI AAGNNNNNCTT 2 cut(s) 1865, 1897
FaqI GGGAC 6 cut(s) 110, 1605, 2646, 6184, 6442, 7125
FauI CCCGC 1 cut(s) 5695
FauNDI CATATG 2 cut(s) 2149, 6334
FbaI TGATCA 2 cut(s) 6807, 7036
FblI GTMKAC 3 cut(s) 225, 4604, 7248
FriOI GRGCYC 7 cut(s) 113, 438, 3257, 3264, 4805, 5265, 5475
FspI TGCGCA 1 cut(s) 4247
GlaI GCGC 8 cut(s) 3357, 4006, 4247, 4659, 5493, 6344, 6660, 7188
GsaI CCCAGC 1 cut(s) 4212
GsuI CTGGAG 6 cut(s) 224, 1159, 1484, 3464, 3699, 4770
HaeII RGCGCY 1 cut(s) 6346
HgaI GACGC 4 cut(s) 2228, 2414, 4901, 6089
HhaI GCGC 8 cut(s) 3358, 4007, 4248, 4660, 5494, 6345, 6661, 7189
Hin6I GCGC 8 cut(s) 3356, 4005, 4246, 4658, 5492, 6343, 6659, 7187
HinP1I GCGC 8 cut(s) 3356, 4005, 4246, 4658, 5492, 6343, 6659, 7187
HincII GTYRAC 7 cut(s) 226, 1102, 2218, 3327, 3918, 4605, 7249
HindII GTYRAC 7 cut(s) 226, 1102, 2218, 3327, 3918, 4605, 7249
HindIII AAGCTT 4 cut(s) 5157, 5501, 5975, 6286
Hpy99I CGWCG 3 cut(s) 125, 6709, 7253
HpyCH4IV ACGT 6 cut(s) 1025, 3826, 4168, 5199, 6687, 6704
HpySE526I ACGT 6 cut(s) 1025, 3826, 4168, 5199, 6687, 6704
HspAI GCGC 8 cut(s) 3356, 4005, 4246, 4658, 5492, 6343, 6659, 7187
Kpn2I TCCGGA 1 cut(s) 5516
KpnI GGTACC 1 cut(s) 4043
Ksp22I TGATCA 2 cut(s) 6807, 7036
LguI GCTCTTC 2 cut(s) 101, 3276
MaeII ACGT 6 cut(s) 1025, 3826, 4168, 5199, 6687, 6704
MfeI CAATTG 4 cut(s) 15, 1457, 4401, 4424
MhlI GDGCHC 8 cut(s) 113, 384, 438, 3257, 3264, 4805, 5265, 5475
MlsI TGGCCA 4 cut(s) 600, 2712, 6318, 6331
MluNI TGGCCA 4 cut(s) 600, 2712, 6318, 6331
MmeI TCCRAC 5 cut(s) 838, 1079, 2415, 2885, 7056
Mox20I TGGCCA 4 cut(s) 600, 2712, 6318, 6331
Mph1103I ATGCAT 6 cut(s) 672, 1350, 1423, 2149, 2291, 5185
MroI TCCGGA 1 cut(s) 5516
MroXI GAANNNNTTC 4 cut(s) 1671, 1776, 5999, 7261
MscI TGGCCA 4 cut(s) 600, 2712, 6318, 6331
Msp20I TGGCCA 4 cut(s) 600, 2712, 6318, 6331
MspA1I CMGCKG 6 cut(s) 2347, 3949, 4820, 4940, 5579, 6782
MspCI CTTAAG 3 cut(s) 1304, 5822, 6236
MunI CAATTG 4 cut(s) 15, 1457, 4401, 4424
Mva1269I GAATGC 6 cut(s) 1050, 1348, 2291, 2856, 5185, 6840
MvnI CGCG 1 cut(s) 3358
NciI CCSGG 4 cut(s) 190, 319, 2576, 4747
NcoI CCATGG 3 cut(s) 4050, 4947, 7106
NdeI CATATG 2 cut(s) 2149, 6334
NmeAIII GCCGAG 2 cut(s) 1489, 2909
NmuCI GTSAC 8 cut(s) 2584, 3894, 4164, 4225, 4330, 4823, 4851, 5443
NsbI TGCGCA 1 cut(s) 4247
NsiI ATGCAT 6 cut(s) 672, 1350, 1423, 2149, 2291, 5185
NspI RCATGY 5 cut(s) 380, 1384, 1720, 4113, 5784
NspV TTCGAA 1 cut(s) 7029
OliI CACNNNNGTG 1 cut(s) 5202
PaeI GCATGC 2 cut(s) 1720, 4113
PagI TCATGA 7 cut(s) 159, 211, 760, 1165, 2026, 3157, 6457
PceI AGGCCT 1 cut(s) 3392
PciI ACATGT 1 cut(s) 376
PciSI GCTCTTC 2 cut(s) 101, 3276
PcsI WCGNNNNNNNCGW 3 cut(s) 516, 1076, 2899
PctI GAATGC 6 cut(s) 1050, 1348, 2291, 2856, 5185, 6840
PdmI GAANNNNTTC 4 cut(s) 1671, 1776, 5999, 7261
PflFI GACNNNGTC 1 cut(s) 4739
PflMI CCANNNNNTGG 3 cut(s) 3486, 4535, 5440
PfoI TCCNGGA 1 cut(s) 3676
PinAI ACCGGT 1 cut(s) 6406
Ppu21I YACGTR 1 cut(s) 4169
PscI ACATGT 1 cut(s) 376
PshBI ATTAAT 2 cut(s) 2276, 5904
PsiI TTATAA 3 cut(s) 2381, 2396, 4176
Psp124BI GAGCTC 5 cut(s) 438, 3264, 4805, 5265, 5475
Psp1406I AACGTT 1 cut(s) 1025
PspFI CCCAGC 1 cut(s) 4208
PspPI GGNCC 7 cut(s) 54, 984, 1944, 2419, 2660, 4676, 6404
PsrI GAACNNNNNNTAC 4 cut(s) 3158, 3190, 6432, 6464
PstI CTGCAG 3 cut(s) 2112, 2458, 4926
PstNI CAGNNNCTG 4 cut(s) 4288, 5699, 6346, 7227
PsyI GACNNNGTC 1 cut(s) 4739
PvuII CAGCTG 6 cut(s) 2347, 3949, 4820, 4940, 5579, 6782
RsaI GTAC 8 cut(s) 1449, 2569, 3119, 3167, 4041, 5360, 5671, 6801
RsaNI GTAC 8 cut(s) 1448, 2568, 3118, 3166, 4040, 5359, 5670, 6800
SacI GAGCTC 5 cut(s) 438, 3264, 4805, 5265, 5475
SalI GTCGAC 3 cut(s) 224, 4603, 7247
SapI GCTCTTC 2 cut(s) 101, 3276
Sau96I GGNCC 7 cut(s) 54, 984, 1944, 2419, 2660, 4676, 6404
ScaI AGTACT 1 cut(s) 2569
SduI GDGCHC 8 cut(s) 113, 384, 438, 3257, 3264, 4805, 5265, 5475
SfcI CTRYAG 6 cut(s) 1032, 2108, 2454, 2741, 4380, 4922
SfuI TTCGAA 1 cut(s) 7029
SinI GGWCC 4 cut(s) 984, 2660, 4676, 6404
SpeI ACTAGT 2 cut(s) 275, 5267
SphI GCATGC 2 cut(s) 1720, 4113
SseBI AGGCCT 1 cut(s) 3392
SspI AATATT 6 cut(s) 496, 3970, 4294, 4972, 5991, 6538
SstI GAGCTC 5 cut(s) 438, 3264, 4805, 5265, 5475
StuI AGGCCT 1 cut(s) 3392
TaiI ACGT 6 cut(s) 1028, 3829, 4171, 5202, 6690, 6707
TaqII GACCGA 1 cut(s) 533
TatI WGTACW 4 cut(s) 1447, 2567, 3117, 5358
TauI GCSGC 1 cut(s) 5138
TseFI GTSAC 8 cut(s) 2584, 3894, 4164, 4225, 4330, 4823, 4851, 5443
Tsp45I GTSAC 8 cut(s) 2584, 3894, 4164, 4225, 4330, 4823, 4851, 5443
Tth111I GACNNNGTC 1 cut(s) 4739
Van91I CCANNNNNTGG 3 cut(s) 3486, 4535, 5440
Vha464I CTTAAG 3 cut(s) 1304, 5822, 6236
VpaK11BI GGWCC 4 cut(s) 984, 2660, 4676, 6404
VspI ATTAAT 2 cut(s) 2276, 5904
XagI CCTNNNNNAGG 2 cut(s) 3623, 6644
XceI RCATGY 5 cut(s) 380, 1384, 1720, 4113, 5784
XcmI CCANNNNNNNNNTGG 3 cut(s) 48, 1798, 4342
XmiI GTMKAC 3 cut(s) 225, 4604, 7248
XmnI GAANNNNTTC 4 cut(s) 1671, 1776, 5999, 7261
ZrmI AGTACT 1 cut(s) 2569
Zsp2I ATGCAT 6 cut(s) 672, 1350, 1423, 2149, 2291, 5185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.