Rw1G018170

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005282
Physical Location & Seq
Forward (+)
39937110 .. 39954514
17405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018170.1

Sequence Viewer

Length: 6336 bp
ATGCTCAAGTTTTCGCAGACCACTGCGAACCAGATTCGGTTCCTCCTGCAAAGCCTCAATGACGCCAACTCGGACTCTGTTCACCGAGAGCTTACTCAGTTTATTGAATATGGAGTTGAGGGAAGCGTTTTGCTGCTCCAAACCTGCCTGGGTCATCTGAATCATTACGGAACAGATACAAAGAATGTGGCACTGGAACGGGTTCTTGCCTCAATTTTCAAATATCTCATCGACCAACCAAATTTCAGTACTATGTTTTGTGAATCACTAAGAAATACAGAGGTCAATGAAGGAGTTGTGGAGAATTTCTCGAGTGTATTGCAACTGTCTGTACCTGAGAAAATTGGCATTGGTCTTGCTTTGTCAGATTCAGAAAACCTAGACACCAGAATATGTGGCAAAAACTTCTGTATGGCTCAGATTGAGAAATTGTGTGAAACTCCTGTTGACAGGATCTCTTCTGAGAAAATTCAGAATATTGTTATGTTCCTTCAGCGGTCTGAGGGTCTTTCCAAGCATGTAGATTCGTTTATGCAGATATTATCTTTACTGCAGCTGAAAGATGATGAAAGCTCTTTTGTTTTAAGTCCATTGCTTTCAGATGAGCTTCGTGATGCCAATTTTTTGAGGAACATGGACTTGTTCCGTGAAAGTGGAGAAAATGATTTTGACGCTATTTTAGCAGAAATGGAGAAGGAGATGAATATGGGAGATATTATGCAGGAATTAGGTTATGGATGCACAGTTGATTCATCTCAGTGCAAAGAGATTTTATCCCTTTTCTTACCCCTCAATGAGATTACTATCTCCAAAATACTTGGCACAATTGCCTGCACCCATGCTGGTCTTGAGGATAATCAGAATACATTTTCGACCTTCCGGTTGGCGCTTGGTTATAGTACTTCATCTGATTTGCCTATGCTAAACACCTGGAACATTGATGTCCTTGTGGATACGATCAATCAACTTGCTCCAGACACCAATTGGATAAGTGTTATTGAAAATTTGGATCATGAGGGGTTCTACATTCCTAATCAGGAGGCATTCTCTTTTTTCATGACTGTGTATCAGCATGTGTGTCAGGAGCCATTCCCCCTACATGCCATCTGTGGGTCGGTTTGGAAGAATACTGAGGGACAGCTGTCTTTTCTCAAACATGCTGTTTCATCACCACCTGAAGTATTTAATTTTGCTCACTCTGTTAGGCAGCTGCCCTATATGGATACGGTGAATGGCCACAAGCTACAACTTGGACATGCAAATCATGCATGGTTGTGTCTAGACCTTTTGGATATTTTGTCGTCCGTCATGGAGATGATTCCTTCTCCCTTTAGTATCAGACTGGCGGCCCTTGCTTTCAGAAAAGAGCTTGTAGAACTCGAGAAGTGGTTGAGTAGTAATTTGAATACATATAAAGATACTTTCTTTGAGGAGTGCCTCAAGTTCCTGAAGGAGATTCAAGCTGGAGGGACCCAAGATTTCTCAACCAGACCTTTCCAACATTCTGGTGCTGTCTCAACTCTCTATGGGGACACTACTGCTACATTCTCGAAGGTTCTTAAAGCTCATGTCGCCCAGATTACTTCTAGCCACCTTACTGAGGAGATGGAAAAACTCTCTCTATCAATTATGGATTCTAATCCAAGGCTGCAGAATGGTGGGCCTACAGAGTCATCAACAGATGGATATGCAGATGACATTGAAGCTGAAGCCAACTCTTACTTCCATCAAATGTTTTCTGGTCAGTTGACTATTGAATCAATGGTCCAAATGCTTGCCCGTTTCAAAGAATCTTCTGTGCAAAGGGAACAATCCATTTTTGAGTGCATGATTGCTAATCTGTTTGAAGAGTACAGATTCTTTCCGAAATATCCTGAAAGACAGCTCAAAATTGCTGCAATTTTATTTGGGTCTGTCATCAAGCAGCAGCTTGTAACTCATCTGACTCTAGGGATTGCTCTGCGTGGTGTTTTAGATGCATTGCGCAAACCTGCGGATTCAAAAATGTTTGTGTTTGGAACAAAGGCTCTGGAGCAGTTTGTGGAGCGTCTGATTGAATGGCCACAGTACTGCAATCACATTCTTCAGATATCCCATTTGCGCAATACTCATTCAGAGCTGGTGGTATTCATTGAACAGGCTCTTGCCAGGATTTCATCTGGTCATTCAGATTCAGATGGAAGTAACCATGCTTCTGCCGTTCATCATCACGGTCCCAGTCAAGTTACCTCAGGAAATGTGGAGTTAAATGCATCAAGCATCTTACAAACTGGGCAGACACTATCTGCTCCGCTCCAGCTCCATCAGAGACATGAAAGCTCTTTTGATGACCGTCACAAGTCCTCAACTCCATCTAATGAGGTAAAGCCACTTTTATCTTCTGCGACGCAACCGGCAGTTGTTCCTGTTAGTGACGCTCCGAGTATTCAGAAGTTACAAAATGCAGTTAGTGCTCCATCAATGTTGTCTTCTGCTTCCCCTGGTTTTGTTCGTCCGTCACGAGGAGCTACCTCTACTAGGTTTGGCTCTGCTTTGAGCATTGAAACATTGGTTGCAGCAGCTGAGAAAAGGGATGCTCCCGTTGAGGCTCCAGCAGAGATTCAGGACAAAGTATTATTTATAGTTAATAATATATCACTTGCTAATATTGAAGTTAAGTCGAAGGAGTTTTCTGAGATACTGAAAGAGCAATACTATCCCTGGTTTGCGCAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCCAATTTTCATGACTTGTACCTGAAATTTCTGGACAAACTTAATTCGAAGGCTTTGAATAAAGAGATTGTTCAAGCCACGTATGAGAACTGCAAGGTTCTTCTAGGGTCTGAGCTTATAAAATCAAGCTCAGAGGAGCGTTCATTGCTAAAAAATTTAGGTAGCTGGCTTGGGAAGCTTACTATTGGAAGGAATCAAGTCTTAAGGGCTCGTGAAATTGATCCCAAATCTTTGATTATAGAGGCATATGAGAAAGGGTTGATGATTGCTGTCATACCTTTTACTTCAAAGATTTTGGAACCATGTCAGAATAGCCTCGCGTATCAACCACCGAATCCCTGGACAATGGGTATTCTTGGATTACTTGCTGAGATCTATTCCATGCCAAACTTGAAGATGAACCTGAAGTTTGATATAGAGGTTCTATTCAAGAATCTAGGAGTGGATTTGAAGGAGATAACACCAACTTCTCTTCTCAAGGATCGCAACAGAGAACTTGAAGGGAATCCAGATTTCTCCAACAAAGATGATGACAAGCTTGCTTTGGGGTTATCTGATCAGATCACTTCTGCTCAAGGACTGTTACAAGCTACTCCATCACAGTCCCCATTTTCTGTTAGTCAGCATCCCACAGCAATACCTAACATTGGAACCCACGTAATTATCAACCAGAAGCTCAGTGCCTTGGGGGGGCTGCACGTGCATTTCCAAAGAGTGGTTCCAATTGCTATGGATAGAGCTATCAAAGAAATTGTCTCTGGTATTGTTCAGCGCAGTGTTTCTATAGCTACCCAGACAACTAAGGAGCTTGTTTTAAAGGACTATGCCATGGAATCAGATGACACAAGAATTTTTACTGCAGCGCACTTGATGGTCGCCAGTTTGGCAGGAAGTCTAGCTCATGTCACATGCAAGGAACCCTTGCGCAGTTCAATATTAAGTCAGCTAAGAAATTCTCTTCAGGGATTAAACATTGCAAGTGATCTTCTAGAACAAGCTGTTCAACTTGTAACCAATGATAATCTGGATCTTGGCTGTGCAGTCATTGAGCAGGCTGCTACAGATAAGGCGGTAGCAACAATTGACGGGGAAATAGGTCCGCAGCTTTCAGTAAGAAGAAAGAGAGAAGGTGTTGGTGCAACCTATTTTGATGCAAACATGTATCCACAAGGTTCTATGGGTGTTGATTTTGTTCGGCTTCCCTGGCAAAACCAATCTATTCAAAGTTCGCACGTTCTGCCAGTTGCTACTACAGCTTCATCTGGTAGTACTGGTCTCACTAGCACATATGGTTCAGCATCTGGACAACTTAACACGGGCTACTCACCAGGACCTGGAAGCAAATTTGAAGCTGTTTCTCGTCCATTAGATGAAGCAATGGAATCCAATTCTGCCCTCCATCTTAGGTTGCTGCACTCTGTAGAGTCATCTGATGCTGTAAAGGAATCTGGAGTGTCCTCACAGCCACCACCTTCACCTGCTGTAACTGAACGTCTTGGAAGTCTCTCAGAACCTTCCCTCACCACAGGGGCTTGCATTGGAAAAATACCAGATTGTTGCACAGAAGCTAACGATGCTAGAGAATCAGAAATCAGGTACTGTATTTCTTTGTTTCCCATATATAGGCTCCCATTTACTTGGAGTTCTCCATTTTGTACTCACACTTGCATTTATACATTCGGCGTCATTGGTGAAGTGCCTGATATTATACTCCGATGTGTAGTCGAGATGAGGCCGCCTTGGCTGTGGCTCAAAAGGTCAGTGCTACTCTTTCGAAGAACGTGTTTTCAAGGGTTTGTACGAGAATGCAACAAACCAGATTCATGTTGGGCACATCTAGCAATGCTGACTGCGATTCGTGATGTTTGCAAGCTTGTGATTTATTCTGACGAGGAGCGGAAGTTTAATAACAAGAAATTACTTGGCCTTATACGCAGTGAACTGCTAAACCTTGCAGAGTACAATGTTCACATGGCAAAACTTATTGATGGTGGCAGGAATAAGCCAGCAACAGAGTTCGCCACTTCCTTGCTCCAAACTTTGGTTATTGAAGAATCTAAAGTTATCTCAGAACTTCAGAGTCTTGTTGATGCAATGGGAAAGGCTGCTGCGAAGCCTGGATCTCCCGAGTCACTGCAGCAGGTGGTTGAAATTGTCAAGAATCCTGCTGCTAATGGAGCTGCTCCATCTGGTGTTAATGTTGGGAAGGATGACAAGGCCAGACAATCGAGAGAGAAAAAGGCTTCTGTTCACTCCCCAGTAAGCAGGGAAGAGTTCAGTATTGTGGAGCCTGCGGAACCAGATCCTCCTGGTTTTCGTGAGCAGGTGTCTATGCTGTTTGCTGAGTGGTACCGGATTTGTGAACTTCCTGGTGCAAACGAAAATAGTTGTGCCCATTTAATCGTACAGTTGCGCTCAAGTGGCATGCTGAAGGGGGATGATCTTACGGACCGATTTTTCCGACTACTCACAGAGCTTACTGTTGCACATTGTCTATCTTCTGAGGTGATTAATTCAGGCACACTGCAAACTCCTCAACAAGTGCAGAATCTATCCTTCCTTGCCATTGATATTTATGCTAAACTTGTATTTTTAATTCTCAAGATGGAGCAGGGATCTGGTAAACTAATGCTCTTGTCCAAGATTTTGGCAGTTACAGTGAGATTTATTCAAAAGGATGCAGAGGAGAAGAAATCATCTTTCAATGCAAGACCGTATTTTAGATTGTTTGTTAACTGGCTGCTAGACCTTGGGTCTTTGGATCCTGTTATTGATGGTGCTAACTTTCAGATATTGACAGCCTTCGCAAATGCATTTCATGCGTTGCAACCCCTCAAAGTTCCTACATTCAGCTTTGCATGGCTGGAGTTGGTGAGTCATAGGAGTTTCATGCCTAAAATGCTAGCTGGAACAAATATGAAAATTGATTTGCTTGCTGAAATCACTTCACGTGTTCTGATTCTGAGGTTGATTTCAACAAGGCAACAAGGTTCTTCATTTTTGACCGAATTGAAGCAAAAATTGCTCCTTTTACCAACCGAAGCTGCCTCAGCTGGTACTCATTACAATGTACCCCTCATCAACTCCCTTGTACTCTATGTGGGGATGCAGGCTATCCAGCAGCTTCAGGCTAGAACACCTCACGCACAGGCAAATCAAACTGTTCCATTTGTATATTTGGTGGGTGCTGCTTTGGATATCTTCCAGACTCTGATAGTGGACCTAGATACTGAAGGACGCTACCTTTTCCTAAACGCAATTGCAAACCAACTGCGCTATCCGAACACCCATACACATTACTTCTCCTTCATTGTTCTTTACTTGTTCGCGGAGTCAAACCAGCAGGAAATTATCCAGGAGCAAATCACAAGGGTATTGTTGGAACGTCTCATTGTTAATCGCCCTCATCCATGGGGTCTACTGATAACATTCATTGAGCTAATCAAGAATCCGAGGTACAACTTCTGGAACCGGGCTTTCATAAGGTGTGCACCAGAGATTGAAAAACTCTTTGAATCTGTATCGAGGTCTTGTGGCGCAAAGCCTGTGGATGAGAGTATGGTTTCAGGTTGGGTACCTGAGACCGCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2111

Amino Acids

234.73

Weight (kDa)

5.7

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 430 - 521 1.3e-15 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 556 - 718 2.1e-67 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 862 - 1079 5.1e-90 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1149 - 1288 6.9e-48 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
NOT1_connector PF23590 1532 - 1633 4.1e-13 CCR4-NOT transcription complex subunit 1, NOT1_connector
ARM_Cnot1 PF25097 1534 - 1635 7.4e-30 Cnot1 ARM repeat
Not1 PF04054 1837 - 1888 6.6e-15 CCR4-Not complex component, Not1
Not1 PF04054 1908 - 2088 4.2e-63 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2861
AarI CACCTGC 3 cut(s) 4228, 4873, 5056
Acc16I TGCGCA 4 cut(s) 1985, 2102, 2709, 3668
Acc36I ACCTGC 5 cut(s) 152, 1999, 4228, 4873, 5056
Acc65I GGTACC 2 cut(s) 5091, 6316
AccB1I GGYRCC 2 cut(s) 5091, 6316
AccB7I CCANNNNNTGG 3 cut(s) 3457, 4076, 4783
AccBSI CCGCTC 3 cut(s) 2293, 4639, 6329
AccI GTMKAC 1 cut(s) 6160
AccII CGCG 2 cut(s) 3062, 6071
AcoI YGGCCR 2 cut(s) 1234, 2060
AcsI RAATTY 9 cut(s) 241, 304, 468, 1003, 2768, 2896, 3591, 3694, 4085
AcvI CACGTG 2 cut(s) 3442, 5693
AcyI GRCGYC 2 cut(s) 63, 4425
AflII CTTAAG 1 cut(s) 2944
AflIII ACRYGT 3 cut(s) 3900, 4523, 5692
AhdI GACNNNNNGTC 1 cut(s) 5494
AjuI GAANNNNNNNTTGG 4 cut(s) 1706, 1738, 3444, 3476
Alw21I GWGCWC 2 cut(s) 2455, 6235
Alw26I GTCTC 7 cut(s) 1519, 2302, 3502, 4022, 4250, 6134, 6317
Alw44I GTGCAC 1 cut(s) 6231
AlwNI CAGNNNCTG 5 cut(s) 2561, 4043, 4076, 4341, 5953
Ama87I CYCGRG 3 cut(s) 310, 1379, 4868
AoxI GGCC 7 cut(s) 1234, 1347, 1661, 2060, 4475, 4666, 4959
ApaLI GTGCAC 1 cut(s) 6231
ApoI RAATTY 9 cut(s) 241, 304, 468, 1003, 2768, 2896, 3591, 3694, 4085
ArsI GACNNNNNNTTYG 2 cut(s) 3480, 3512
AseI ATTAAT 1 cut(s) 5253
Asp700I GAANNNNTTC 1 cut(s) 201
Asp718I GGTACC 2 cut(s) 5091, 6316
AspS9I GGNCC 9 cut(s) 1348, 1470, 1661, 1765, 2213, 3839, 4073, 5191, 5962
AsuC2I CCSGG 1 cut(s) 6215
AsuHPI GGTGA 9 cut(s) 74, 1161, 1240, 4059, 4209, 4255, 4445, 5260, 5626
AsuII TTCGAA 2 cut(s) 2789, 4516
AsuNHI GCTAGC 1 cut(s) 5644
AvaI CYCGRG 3 cut(s) 310, 1379, 4868
AvaII GGWCC 7 cut(s) 1470, 1765, 2213, 3839, 4073, 5191, 5962
AxyI CCTNAGG 1 cut(s) 2230
BaeGI GKGCMC 3 cut(s) 4576, 5137, 6235
BalI TGGCCA 2 cut(s) 1236, 2062
BamHI GGATCC 1 cut(s) 5503
BanI GGYRCC 2 cut(s) 5091, 6316
BanII GRGCYC 2 cut(s) 2745, 2953
BarI GAAGNNNNNNTAC 2 cut(s) 2176, 2208
BauI CACGAG 2 cut(s) 2499, 2952
BbrPI CACGTG 2 cut(s) 3442, 5693
BbsI GAAGAC 1 cut(s) 2460
Bbv12I GWGCWC 2 cut(s) 2455, 6235
BbvCI CCTCAGC 1 cut(s) 5791
BceAI ACGGC 1 cut(s) 2183
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciVI GTATCC 3 cut(s) 946, 1216, 3915
BclI TGATCA 1 cut(s) 3298
BcnI CCSGG 1 cut(s) 6215
BcoDI GTCTC 7 cut(s) 1519, 2302, 3502, 4022, 4250, 6134, 6317
BfmI CTRYAG 9 cut(s) 551, 1649, 1665, 3525, 3600, 3801, 3993, 4161, 4877
BfoI RGCGCY 1 cut(s) 890
BfrI CTTAAG 1 cut(s) 2944
BfuAI ACCTGC 5 cut(s) 152, 1999, 4228, 4873, 5056
BfuI GTATCC 3 cut(s) 946, 1216, 3915
BglI GCCNNNNNGGC 2 cut(s) 3626, 4483
BglII AGATCT 1 cut(s) 3114
BmcAI AGTACT 4 cut(s) 250, 901, 2069, 4012
Bme18I GGWCC 7 cut(s) 1470, 1765, 2213, 3839, 4073, 5191, 5962
BmeRI GACNNNNNGTC 1 cut(s) 5494
BmeT110I CYCGRG 3 cut(s) 310, 1379, 4868
BmgT120I GGNCC 9 cut(s) 1348, 1470, 1661, 1765, 2213, 3839, 4073, 5191, 5962
BmrI ACTGGG 3 cut(s) 2211, 2280, 4994
BmtI GCTAGC 1 cut(s) 5648
BmuI ACTGGG 3 cut(s) 2211, 2280, 4994
BoxI GACNNNNGTC 1 cut(s) 1141
BpiI GAAGAC 1 cut(s) 2460
BplI GAGNNNNNCTC 4 cut(s) 293, 325, 1031, 1063
BpmI CTGGAG 7 cut(s) 957, 1485, 2051, 2279, 2574, 4212, 5627
Bpu10I CCTNAGC 1 cut(s) 5791
Bpu14I TTCGAA 2 cut(s) 2789, 4516
BpuEI CTTGAG 6 cut(s) 869, 1424, 3203, 3300, 5143, 5327
BpuMI CCSGG 1 cut(s) 6215
BsaAI YACGTR 4 cut(s) 2823, 3400, 3442, 5693
BsaBI GATNNNNATC 2 cut(s) 803, 1638
BsaHI GRCGYC 2 cut(s) 63, 4425
BsaI GGTCTC 2 cut(s) 4022, 6317
BsaWI WCCGGW 2 cut(s) 879, 5094
BsaXI ACNNNNNCTCC 2 cut(s) 4628, 4658
Bse118I RCCGGY 1 cut(s) 2392
Bse1I ACTGG 9 cut(s) 198, 1347, 2217, 2275, 3621, 3983, 4018, 5000, 5483
Bse21I CCTNAGG 1 cut(s) 2230
Bse3DI GCAATG 7 cut(s) 590, 1979, 2885, 3714, 4125, 4590, 4842
Bse8I GATNNNNATC 2 cut(s) 803, 1638
BseGI GGATG 9 cut(s) 743, 2578, 3367, 4957, 5185, 5425, 5853, 6148, 6298
BseJI GATNNNNATC 2 cut(s) 803, 1638
BseMI GCAATG 7 cut(s) 590, 1979, 2885, 3714, 4125, 4590, 4842
BseNI ACTGG 9 cut(s) 198, 1347, 2217, 2275, 3621, 3983, 4018, 5000, 5483
BseRI GAGGAG 7 cut(s) 1445, 1616, 2517, 2891, 4649, 5265, 5441
BseSI GKGCMC 3 cut(s) 4576, 5137, 6235
BsgI GTGCAG 5 cut(s) 817, 3422, 3801, 4139, 5306
Bsh1236I CGCG 2 cut(s) 3062, 6071
BshFI GGCC 7 cut(s) 1236, 1349, 1663, 2062, 4477, 4668, 4961
BshNI GGYRCC 2 cut(s) 5091, 6316
BsiHKAI GWGCWC 2 cut(s) 2455, 6235
BsiHKCI CYCGRG 3 cut(s) 310, 1379, 4868
BsiSI CCGG 4 cut(s) 880, 2393, 5095, 6214
BslFI GGGAC 5 cut(s) 1149, 1483, 1544, 2199, 3331
BsmAI GTCTC 7 cut(s) 1519, 2302, 3502, 4022, 4250, 6134, 6317
BsmBI CGTCTC 1 cut(s) 6134
BsmFI GGGAC 5 cut(s) 1149, 1483, 1544, 2199, 3331
BsmI GAATGC 2 cut(s) 1043, 4553
BsnI GGCC 7 cut(s) 1236, 1349, 1663, 2062, 4477, 4668, 4961
Bso31I GGTCTC 2 cut(s) 4022, 6317
BsoBI CYCGRG 3 cut(s) 310, 1379, 4868
Bsp119I TTCGAA 2 cut(s) 2789, 4516
Bsp1286I GDGCHC 6 cut(s) 2455, 2745, 2953, 4576, 5137, 6235
Bsp19I CCATGG 2 cut(s) 3570, 6152
BspANI GGCC 7 cut(s) 1236, 1349, 1663, 2062, 4477, 4668, 4961
BspFNI CGCG 2 cut(s) 3062, 6071
BspHI TCATGA 3 cut(s) 1012, 1056, 2752
BspMAI CTGCAG 4 cut(s) 555, 1653, 3604, 4881
BspMI ACCTGC 5 cut(s) 152, 1999, 4228, 4873, 5056
BspOI GCTAGC 1 cut(s) 5648
BspT104I TTCGAA 2 cut(s) 2789, 4516
BspT107I GGYRCC 2 cut(s) 5091, 6316
BspTI CTTAAG 1 cut(s) 2944
BspTNI GGTCTC 2 cut(s) 4022, 6317
BsrBI CCGCTC 3 cut(s) 2293, 4639, 6329
BsrDI GCAATG 7 cut(s) 590, 1979, 2885, 3714, 4125, 4590, 4842
BsrFI RCCGGY 1 cut(s) 2392
BsrI ACTGG 9 cut(s) 198, 1347, 2217, 2275, 3621, 3983, 4018, 5000, 5483
BssAI RCCGGY 1 cut(s) 2392
BssNI GRCGYC 2 cut(s) 63, 4425
BssSI CACGAG 2 cut(s) 2499, 2952
BssT1I CCWWGG 6 cut(s) 1643, 3426, 3570, 4481, 5491, 6152
Bst2BI CACGAG 2 cut(s) 2499, 2952
Bst6I CTCTTC 5 cut(s) 463, 1842, 3219, 3705, 5007
BstACI GRCGYC 2 cut(s) 63, 4425
BstAFI CTTAAG 1 cut(s) 2944
BstAPI GCANNNNNTGC 5 cut(s) 1265, 2450, 3979, 5561, 5764
BstBAI YACGTR 4 cut(s) 2823, 3400, 3442, 5693
BstBI TTCGAA 2 cut(s) 2789, 4516
BstDSI CCRYGG 2 cut(s) 3570, 6152
BstENI CCTNNNNNAGG 2 cut(s) 1598, 2515
BstF5I GGATG 9 cut(s) 743, 2578, 3367, 4957, 5185, 5425, 5853, 6148, 6298
BstFNI CGCG 2 cut(s) 3062, 6071
BstH2I RGCGCY 1 cut(s) 890
BstMAI GTCTC 7 cut(s) 1519, 2302, 3502, 4022, 4250, 6134, 6317
BstNSI RCATGY 8 cut(s) 521, 1076, 1103, 1160, 1259, 3654, 3904, 5170
BstPAI GACNNNNGTC 1 cut(s) 1141
BstSFI CTRYAG 9 cut(s) 551, 1649, 1665, 3525, 3600, 3801, 3993, 4161, 4877
BstSLI GKGCMC 3 cut(s) 4576, 5137, 6235
BstUI CGCG 2 cut(s) 3062, 6071
BstV2I GAAGAC 1 cut(s) 2460
BstX2I RGATCY 7 cut(s) 453, 3114, 3769, 4862, 5044, 5357, 5503
BstXI CCANNNNNNTGG 3 cut(s) 1505, 4380, 5389
BstYI RGATCY 7 cut(s) 453, 3114, 3769, 4862, 5044, 5357, 5503
Bsu36I CCTNAGG 1 cut(s) 2230
BsuI GTATCC 3 cut(s) 946, 1216, 3915
BsuRI GGCC 7 cut(s) 1236, 1349, 1663, 2062, 4477, 4668, 4961
BtgI CCRYGG 2 cut(s) 3570, 6152
BtsCI GGATG 9 cut(s) 743, 2578, 3367, 4957, 5185, 5425, 5853, 6148, 6298
BtsI GCAGTG 5 cut(s) 21, 3523, 4684, 4874, 5264
BveI ACCTGC 5 cut(s) 152, 1999, 4228, 4873, 5056
CaiI CAGNNNCTG 5 cut(s) 2561, 4043, 4076, 4341, 5953
CciI TCATGA 3 cut(s) 1012, 1056, 2752
Cfr10I RCCGGY 1 cut(s) 2392
Cfr13I GGNCC 9 cut(s) 1348, 1470, 1661, 1765, 2213, 3839, 4073, 5191, 5962
CpoI CGGWCCG 1 cut(s) 5191
CseI GACGC 7 cut(s) 71, 680, 2036, 2395, 2423, 4414, 5988
CspCI CAANNNNNGTGG 4 cut(s) 168, 203, 6270, 6305
CspI CGGWCCG 1 cut(s) 5191
DraI TTTAAA 1 cut(s) 3558
DriI GACNNNNNGTC 1 cut(s) 5494
EaeI YGGCCR 2 cut(s) 1234, 2060
Eam1104I CTCTTC 5 cut(s) 463, 1842, 3219, 3705, 5007
Eam1105I GACNNNNNGTC 1 cut(s) 5494
EarI CTCTTC 5 cut(s) 463, 1842, 3219, 3705, 5007
Eco130I CCWWGG 6 cut(s) 1643, 3426, 3570, 4481, 5491, 6152
Eco24I GRGCYC 2 cut(s) 2745, 2953
Eco31I GGTCTC 2 cut(s) 4022, 6317
Eco32I GATATC 2 cut(s) 2091, 5941
Eco47I GGWCC 7 cut(s) 1470, 1765, 2213, 3839, 4073, 5191, 5962
Eco72I CACGTG 2 cut(s) 3442, 5693
Eco81I CCTNAGG 1 cut(s) 2230
Eco88I CYCGRG 3 cut(s) 310, 1379, 4868
EcoNI CCTNNNNNAGG 2 cut(s) 1598, 2515
EcoO109I RGGNCCY 2 cut(s) 1470, 4073
EcoRV GATATC 2 cut(s) 2091, 5941
EcoT14I CCWWGG 6 cut(s) 1643, 3426, 3570, 4481, 5491, 6152
EcoT22I ATGCAT 4 cut(s) 1270, 1981, 2254, 5557
EcoT38I GRGCYC 2 cut(s) 2745, 2953
ErhI CCWWGG 6 cut(s) 1643, 3426, 3570, 4481, 5491, 6152
Esp3I CGTCTC 1 cut(s) 6134
FalI AAGNNNNNCTT 2 cut(s) 3647, 3679
FaqI GGGAC 5 cut(s) 1149, 1483, 1544, 2199, 3331
FauNDI CATATG 2 cut(s) 2989, 4030
FbaI TGATCA 1 cut(s) 3298
FblI GTMKAC 1 cut(s) 6160
FokI GGATG 9 cut(s) 750, 2585, 3354, 4964, 5192, 5432, 5860, 6135, 6305
FriOI GRGCYC 2 cut(s) 2745, 2953
FspI TGCGCA 4 cut(s) 1985, 2102, 2709, 3668
GsuI CTGGAG 7 cut(s) 957, 1485, 2051, 2279, 2574, 4212, 5627
HaeII RGCGCY 1 cut(s) 890
HaeIII GGCC 7 cut(s) 1236, 1349, 1663, 2062, 4477, 4668, 4961
HapII CCGG 4 cut(s) 880, 2393, 5095, 6214
HgaI GACGC 7 cut(s) 71, 680, 2036, 2395, 2423, 4414, 5988
Hin1I GRCGYC 2 cut(s) 63, 4425
HincII GTYRAC 3 cut(s) 448, 1749, 5476
HindII GTYRAC 3 cut(s) 448, 1749, 5476
HindIII AAGCTT 3 cut(s) 2918, 3278, 4613
HpaI GTTAAC 1 cut(s) 5476
HpaII CCGG 4 cut(s) 880, 2393, 5095, 6214
HphI GGTGA 9 cut(s) 74, 1161, 1240, 4059, 4209, 4255, 4445, 5260, 5626
Hpy99I CGWCG 1 cut(s) 2389
HpyCH4IV ACGT 8 cut(s) 2822, 3399, 3441, 3975, 4234, 4523, 5692, 6127
HpySE526I ACGT 8 cut(s) 2822, 3399, 3441, 3975, 4234, 4523, 5692, 6127
Hsp92I GRCGYC 2 cut(s) 63, 4425
KflI GGGWCCC 1 cut(s) 1470
KpnI GGTACC 2 cut(s) 5095, 6320
Ksp22I TGATCA 1 cut(s) 3298
KspAI GTTAAC 1 cut(s) 5476
MaeII ACGT 8 cut(s) 2822, 3399, 3441, 3975, 4234, 4523, 5692, 6127
MbiI CCGCTC 3 cut(s) 2293, 4639, 6329
MfeI CAATTG 5 cut(s) 825, 982, 3465, 3822, 6000
MflI RGATCY 7 cut(s) 453, 3114, 3769, 4862, 5044, 5357, 5503
MhlI GDGCHC 6 cut(s) 2455, 2745, 2953, 4576, 5137, 6235
MlsI TGGCCA 2 cut(s) 1236, 2062
MluNI TGGCCA 2 cut(s) 1236, 2062
MlyI GAGTC 9 cut(s) 68, 1679, 1939, 4175, 4831, 4880, 5626, 5944, 6083
MmeI TCCRAC 4 cut(s) 1522, 3285, 5227, 6102
Mox20I TGGCCA 2 cut(s) 1236, 2062
Mph1103I ATGCAT 4 cut(s) 1270, 1981, 2254, 5557
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 2 cut(s) 1236, 2062
MslI CAYNNNNRTG 6 cut(s) 757, 1061, 1273, 1313, 1506, 5808
Msp20I TGGCCA 2 cut(s) 1236, 2062
MspA1I CMGCKG 6 cut(s) 496, 556, 1141, 1210, 2561, 5795
MspCI CTTAAG 1 cut(s) 2944
MspI CCGG 4 cut(s) 880, 2393, 5095, 6214
MunI CAATTG 5 cut(s) 825, 982, 3465, 3822, 6000
Mva1269I GAATGC 2 cut(s) 1043, 4553
MvnI CGCG 2 cut(s) 3062, 6071
NciI CCSGG 1 cut(s) 6215
NcoI CCATGG 2 cut(s) 3570, 6152
NdeI CATATG 2 cut(s) 2989, 4030
NheI GCTAGC 1 cut(s) 5644
NmuCI GTSAC 5 cut(s) 2333, 2411, 2496, 3646, 4872
NsbI TGCGCA 4 cut(s) 1985, 2102, 2709, 3668
NsiI ATGCAT 4 cut(s) 1270, 1981, 2254, 5557
NspI RCATGY 8 cut(s) 521, 1076, 1103, 1160, 1259, 3654, 3904, 5170
NspV TTCGAA 2 cut(s) 2789, 4516
PaeI GCATGC 1 cut(s) 5170
PaeR7I CTCGAG 2 cut(s) 310, 1379
PagI TCATGA 3 cut(s) 1012, 1056, 2752
PaqCI CACCTGC 3 cut(s) 4228, 4873, 5056
PciI ACATGT 1 cut(s) 3900
PcsI WCGNNNNNNNCGW 1 cut(s) 2497
PctI GAATGC 2 cut(s) 1043, 4553
PdmI GAANNNNTTC 1 cut(s) 201
PflMI CCANNNNNTGG 3 cut(s) 3457, 4076, 4783
PfoI TCCNGGA 1 cut(s) 6096
PleI GAGTC 9 cut(s) 68, 1678, 1939, 4174, 4830, 4879, 5625, 5944, 6082
PmaCI CACGTG 2 cut(s) 3442, 5693
PmlI CACGTG 2 cut(s) 3442, 5693
PpsI GAGTC 9 cut(s) 68, 1678, 1939, 4174, 4830, 4879, 5625, 5944, 6082
Ppu21I YACGTR 4 cut(s) 2823, 3400, 3442, 5693
PpuMI RGGWCCY 2 cut(s) 1470, 4073
PscI ACATGT 1 cut(s) 3900
PshAI GACNNNNGTC 1 cut(s) 1141
PshBI ATTAAT 1 cut(s) 5253
PsiI TTATAA 1 cut(s) 2861
Psp5II RGGWCCY 2 cut(s) 1470, 4073
PspCI CACGTG 2 cut(s) 3442, 5693
PspPI GGNCC 9 cut(s) 1348, 1470, 1661, 1765, 2213, 3839, 4073, 5191, 5962
PspPPI RGGWCCY 2 cut(s) 1470, 4073
PstI CTGCAG 4 cut(s) 555, 1653, 3604, 4881
PstNI CAGNNNCTG 5 cut(s) 2561, 4043, 4076, 4341, 5953
PsuI RGATCY 7 cut(s) 453, 3114, 3769, 4862, 5044, 5357, 5503
PvuII CAGCTG 5 cut(s) 556, 1141, 1210, 2561, 5795
RseI CAYNNNNRTG 6 cut(s) 757, 1061, 1273, 1313, 1506, 5808
Rsr2I CGGWCCG 1 cut(s) 5191
RsrII CGGWCCG 1 cut(s) 5191
Sau96I GGNCC 9 cut(s) 1348, 1470, 1661, 1765, 2213, 3839, 4073, 5191, 5962
ScaI AGTACT 4 cut(s) 250, 901, 2069, 4012
SchI GAGTC 9 cut(s) 68, 1679, 1939, 4175, 4831, 4880, 5626, 5944, 6083
SduI GDGCHC 6 cut(s) 2455, 2745, 2953, 4576, 5137, 6235
SfcI CTRYAG 9 cut(s) 551, 1649, 1665, 3525, 3600, 3801, 3993, 4161, 4877
Sfr274I CTCGAG 2 cut(s) 310, 1379
SfuI TTCGAA 2 cut(s) 2789, 4516
SinI GGWCC 7 cut(s) 1470, 1765, 2213, 3839, 4073, 5191, 5962
SlaI CTCGAG 2 cut(s) 310, 1379
SmiMI CAYNNNNRTG 6 cut(s) 757, 1061, 1273, 1313, 1506, 5808
SphI GCATGC 1 cut(s) 5170
SspI AATATT 3 cut(s) 478, 2647, 3678
StyI CCWWGG 6 cut(s) 1643, 3426, 3570, 4481, 5491, 6152
TaiI ACGT 8 cut(s) 2825, 3402, 3444, 3978, 4237, 4526, 5695, 6130
TaqII GACCGA 2 cut(s) 5208, 5762
TatI WGTACW 8 cut(s) 248, 899, 1851, 2067, 4010, 4397, 4701, 5833
TauI GCSGC 2 cut(s) 1349, 4480
TseFI GTSAC 5 cut(s) 2333, 2411, 2496, 3646, 4872
Tsp45I GTSAC 5 cut(s) 2333, 2411, 2496, 3646, 4872
TspGWI ACGGA 5 cut(s) 183, 635, 1294, 2484, 5204
Van91I CCANNNNNTGG 3 cut(s) 3457, 4076, 4783
Vha464I CTTAAG 1 cut(s) 2944
VneI GTGCAC 1 cut(s) 6231
VpaK11BI GGWCC 7 cut(s) 1470, 1765, 2213, 3839, 4073, 5191, 5962
VspI ATTAAT 1 cut(s) 5253
XagI CCTNNNNNAGG 2 cut(s) 1598, 2515
XapI RAATTY 9 cut(s) 241, 304, 468, 1003, 2768, 2896, 3591, 3694, 4085
XbaI TCTAGA 2 cut(s) 1279, 3730
XceI RCATGY 8 cut(s) 521, 1076, 1103, 1160, 1259, 3654, 3904, 5170
XcmI CCANNNNNNNNNTGG 4 cut(s) 981, 3078, 3763, 4566
XhoI CTCGAG 2 cut(s) 310, 1379
XmiI GTMKAC 1 cut(s) 6160
XmnI GAANNNNTTC 1 cut(s) 201
ZrmI AGTACT 4 cut(s) 250, 901, 2069, 4012
Zsp2I ATGCAT 4 cut(s) 1270, 1981, 2254, 5557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.