FvH4_7g10770

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005282
Physical Location & Seq
Forward (+)
10103619 .. 10121357
17739 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g10770.t6

Sequence Viewer

Length: 7200 bp
ATGCTCAAGTTTTCGCAGACCACTGCGAACCAGATTCGCTTCCTCCTCCAAAGCCTCAACGACGCCAACTCCGACTCTGTTCTCCGCGAGCTCACTCAGTTTATTGAATATGGAATTGAGGGAAGCATTTTGCTGCTCCAAACCTGCCTGGATCATCTGAATCATTACAGAATAGATATAAAGAATGTGGCACTGGAACGGGTTCTTGCCTCACTTTTCAAATATCTCATTGACCAACCAAATTTCAGTACTATGTTCTGTGAATCACTAAGAAATACAGAGGTCAATGAAGGAATTGTTGAGAATTTCTCGAGTGCATTGCAACTGTCTGTACCTGAGAAAATTGGCATTGGTCTTGCCTTGTCAGATTCAGAAAACCTAGACACCAGAATATGTGGCAAGAACTTCTGTATGGCTCAGATTGAGCAATTATGTGAAACTCCTGTTGACAAGATCTCTTCCGAGAAAATTCAGAGTATTGTTTTGTTCCTCCAGCGGTCTGAGGGTCTTTCCAAGCATGTAGATTCCTTTATGCAGATGTTATCTTTACTGCAGCTGAAAGATGATGAAAGCTCATTTGTGTTAAGTCCATTGCTTTCAAATGAGCTTCGTGATGACAATTTTTTGAGGAACGTGGATTTGTTCCATGAAAGTGGAGAAAATGATTTTGATGCGATTTTAGCTGAAATGGAGAAGGAGATGAGCATGGGAGATATTATGAAGGAATTAGGTTATGGATGCACAGTTGATTCATCCCAGTGCAAAGAGATTTTATCCCTTTTCTTACCTCTCAATGAGATTACTATCTCCAAAATACTTGGCACAATTGCCTGCACCCATGCTGGTCTCGAGGATAATCAGAATACGTTTTCAACCTTTCGATTGGCAATGGGTTACAGCACTTCATCTGATTTGCCTATGCTAAACACCTGGAACATTGATGTCCTCGTGGATACGATCAATCAACTTGCTCCGGACACCAACTGGATAAGTGTTATTGAAAATTTGGATCACGAGGGCTTCTACATTCCTAATAAGGAGGCATTCTCTTTTTTCATGTCTGTATATCAGCATGTCTGTCAGGAGCCCTTCCCCCTACATGCTATCTGTGGGTCTGTTTGGAAGAATACTGACGGTCAGCTATCATTTCTCAAACATGCTGTTTCATCACCACCTGAAGTATTTAACTTTGCTCACTCTGTTAGGCAGCTGCCCTATGTGGATGCAGTGAATGGTCACAAGCTACAACTTGGGCATGCAAATCATGCATGGTTGTGTCTAGACCTTTTGGATGTACTTTGTCACCTAGCAGAGAGGGGTCATGCTCTTTCCGTTCGATCGATACTTGAGTATCCTCTTCAACACTGTCCTGAAGTCCTGCTTCTTGGGATGGCACATATTAATACTCCATACAACCTGCTGCAATATGAAGTGTCTTTTATTGTTTTCCCCATGATAGTAAAAAATGTCATGGGCAATGGCATGATCGTTCACCTCTGGCATACTAACCCCTCTTTGGTGCTCCGGGGATTTATGGATACTTACAATAGTGATCCAGAGAGCATGACTAGGATATTGGACATCTGTCAAGAGCTTAAGATTTTGTCGTCCGTCATGGAGATGATTCCTTCTCCCTTTAGTATCAGACTGGCGGCCCTTGCTTTCAGAAAAGAGCTTGTAGAACTGGAGAAGTGGTTGAACAGTAATTTGATTACATATAAAGATACTTTCTTTGAGGAGTGCCTCAAGTTCCTAAAGGAGATTCAAGCTGGTGGGACCCAAGATTTCTCTACCAGACCTTTCCAACATTCTGGTGCTGTCTCAACTCTCTATGGGGACACTACTGCTACCTTCTCAAAGGTTCTGAAAGCTCATGTCGGGCAGATTACTTCTAGCCACCTTACTGAGGACTTGGAGAAACTCTCTGTGTCGATTATGGATTCTAATCCAAGGCTACCGAATGGTGGGTCTACAGAGTCATCAACAGATGGATATGCAGATGATATTGAAACTGAAGCCAACTCTTACTTCCATCAAATGTTTTCTGGTCAGTTGACTATTGAATCAATGGTCCAAATGCTTGCTCGTTTCAAAGAATCTTCTGTACAAAGGGAACAATCCATTTTTGAGTGCATGATTGCCAATCTGTTTGAGGAGTATAGATTCTTTCCGAAGTATCCTGAAAGACAGCTCAAAATTGCCGCGATTTTATTTGGGTCTGTCATCAAGCAGCAGCTTGTAACTCATCTGACTCTAGGGATTGCTCTGCGTGGTGTTTTAGATGCATTGCGCAAACCTGCAGATTCAAAAATGTTTGTGTTCGGAACCATGGCTTTGGAGCAGTTTGTGGAGCGTCTGATTGAGTGGCCACAGTATTGCAATCACATTCTTCAGATATCCCATTTGCGCAATACTCATTCAGAGCTGGTGGTATTCATTGAACAGGCTCTTGCCAGGATTTCTTCCGGCAATTCAGATCCAGAGGGAAGTAACCATGCTTCTGCAGTTCATCATCACGGCCCCAGTCAAGTTACCTCAGGAAATGTGGAGTTAAATGGATCTAGCATCTTACCAACTGGGCAGACGCTATCTTCTTCACTCCAGCTCCAACAGAGACATGAAAGCTCTCTTGATGATCGTCTCAAGTCCTCAACTTCATCGAATGAGGTAAAACCACTTTTATCTTCTGCGATGCAACCTGCAGCTCCTGTTGGTGATGCTCCGAGTATTCAGAAGTTACAAATTGCAGTTAGTGCTCCATCAATGTTGTCCACTGTTTCCCCTGGTCTTGTTCGTCCATCACGAGGAGCTACCTCTACCAGGTTTGGCTCCGCTTTGAGCATTGAAACATTGGTTGCAGCAGCTGAGAAAAGAGACGCTCCCGTTGAGGCTCCAGCAGAGATTCAGGACAAAGTATTATTTATAGTTAATAATATATCACTTGCTAATATTGAAGTCAAGTCAAAGGAGTTCTCTGAGATACTGAAAGAGCAATACTATCCTTGGTTTGCACAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCAAATTTTCATGACATGTATCTGAAATTTCTGGACAAACTTAATTCGAAGGCTTTGAATAAAGAGATTGTTCAAGCCACGTATGAGAACTGCAAGGTTCTTCTAGGGTCGGAGCTTATAAAATCAAGCTCAGAGGAGCGTTCGTTGTTAAAAAATTTAGGTAGCTGGCTTGGGAAACTTACGATCGGAAGGAATCAAGTCTTAAGGGCTCGTGAAATTGATCCCAAATCTTTGATCATAGAGGCATATGAGAAAGGTTTGATGATTGCAGTCATACCTTTCACTTCAAAGATTTTGGAACCATGTCAGAGTAGCCTGGCGTATCAACCACCAAATCCCTGGACAATGGGTATTCTTGGATTACTTGCTGAGATCTATTCCATGCCAAACTTGAAGATGAACCTCAAGTTTGATATAGAGGTTCTATTCAAGAATCTAGGAGTGGATTTGAAGGAGATAACACCAACTTCTCTTCTCAAGAATCGCACCAGAGAACTTGAAGGGAATCCTGATTTCTCCAACAAAGATGTTGGAGCAACCCAACCACAAATTGCTGAAGTAAAATCTGGAATTATCTCTCCACTAAACCAAGTTGAGCTACCACTTGACGTTGCTCCATCTAATTCTGGGAGCCACACACATTTGTTATCTCAGTATGCTACGTTGATGGAGGATGACAAGCTTGCTTTGGGATTATCTGATCAGATCACTTCTGCTCAAGGACTGTTACAAGCTTCTCCCTCACAGTCCCCATTTTCTGTTAGTCAGCATCCCACAGCAATACCTAACATTGGAACCCGCGTAGTTATCAACCAAAAACACAGTGGCTTGGGGGGGTTGCACGTGCATTTCCAAAGAGTGGTTCCAATTGCTATGGATAGAGCTATCAAAGAAATTGTCTCTGGTATTGTTCAGCGCAGTGTTTCTATAGCTACTCAGACAACTAAGGAGCTTGTGTTAAAGGACTATGCCATGGAATCAGATGACACAAGAATATTGAATGCAGCTCACTTGATGGTCGCAAGTTTGGCCGGAAGTCTTGCACATGTAACATGCAAGGAACCCTTGAGGAGTTCAATATTAAGTCAGCTAAGAAATTCTCTTCAGGGATTAAACATCGCAAGTGAATATCTAGAACAAGCTGTTCAACTTGTAACCAATGATAATCTGGATCTTGGATGTGCAGTCATTGAGCAGGCTGCTACAGATAAGGCAGTAGCAACAATTGATGGTGAGATAGGTCAGCAGCTTTCAGTAAGAAGAAAGAGAGAAGGTGTTGGTGCAACCTATTTTGATGCTAACATGTATCCACAAGGTTCTATGGGTGTTGTGCCAGAAGCTCTCCGCCCTAAACCTGGTCATTTGTCCCTTTCTCAACAACGAGTGTATGAGGATTTTGTTCGGATTCCCTGGCAAAACCAATCTATTCAAAGTTCGCATGTTCTGCCAGTTGCTACCACAGCTTCATCTGGTAGTGCCGGTCTCACTAGCACATATGGTTCAGCATCCGGGCAACTTAACACGGGCTACTCACCAGGACCTGGAAGCAAATTTGAAGCTGTTTCTCGTCCATTGGATGAAGTGATGGAATCCAATTCTGCCCTCCATCTTAGTGCTTCATCTATTCATGTTGGGGTGGGCGATGGCGTTTCCCATGATAGTTCTGAAAATGATTCTGTTGTTGGCTCATTTCCTTCCGCTGCTTCTGCCCCCGAGCTGCACTCTGTAGAGTCATCTGATGCTGTAAAGGAATCTGGAGTATCTTCACAGCCACTGCCTTCACCTGCTGTAACTGAACGTCTTGGAAATCTCTCAGAGCCTTCCCTCAGCACAGGGGCTGCATTGGAAAAATACCAGATTGTTGCGCAGAAGTTGGAAGCCTTGGTAACTGACGATGCTAGAGAATCAGAAATCCAGGGAGTCATTGGTGAAGTGCCTGATATTATACTCAGATGTGTTAGTCGAGATGAGGCTGCCTTGGCTGTGGCTCAAAAGGTTTTCAAGGGTTTGTATGAGAATGCATCAAACCAGATTCATGTTGGCGCTCATCTCGCAATGCTTACTGCGATTCGTGATGTTTGCAAGCTTGTTTTTACTCAGCTAACCAGTTGGGTGATTTATTCTGACGAGGAGCGGAAGTTTAACAAAGAAATTACTGTTGGCCTTATACGCAGTGAATTGCTAAACCTTGCAGAGTACAATGTTCACATGGCAAAACTTATTGACGGTGGCAAGAATAAGGCAGCAACAGAGTTTGCCACTTCCTTGATCCAAACTTTGGTGATTGAAGAATATAAAGTTATCTCAGAACTTAGGAATCTTGTTGAAGCATTGTTTAAGGTTGCTGCAAAGCCTGGATCTCCTGAGTCACTGCAGCAGCTGGTTGAAACTATCAAGAATCCTGCTGCAAATGTGGCTGCTCTATCTGGTATTAATGTTGGGAAGGACGACAAGGCCAGACAATCGAGAGAGAAGAAGGCCCCTGTCCACTCCCCGGGAAGCAGGGAAGAGTTCAGTATTGTGGAGTCTGCAGAACCAGATCCACCTGGTTTTCCCGAGCAGGTGAAAGATGTGTTTGCTCAGTGGTGCCGGTTTTGTGAACTTCCTGGTGCAAACGAAAATAATTGTTCCCATTTTATCTTAAATTTGCACTCGATTGGCATCCTGAAGGATGATCTGACGGACCGGTTTTTCCGACTACTCACAGAGATTTCTGTTGAACATTGTCTATCTTCTGAGGTGATTAATTCAGGCACACTGCAAACTCCTCAGCAAGCGCAGAGTCTATCCTTCCTGGCCATTGATATTTATGCTAAACTTGTATTTTCAATTCTCAAGATGGAGCAGGGATCCAGTAAATTGATTCTCTTGTCCAAGATTTTGGCAGTCACAGTGAGATTCATTCAGAAGGATGCAGAGGAGAAGAAATCGTCTTTCAATGCAAGACCATATTTTAGATTGTTTGTTAACTGGTTGCTAGACCTTGGGTCTTTGGATCCCGTTATTGATGGTGCTAACTTTCAGATATTGATAGCCTTTGCAAATGCGTTTCATGCGTTGCAACCCCTCAAAGTTCCTACATTCAGCTTTGCATGGCTCGAGCTGGTGAGTCATAGGAGTTTCATGCCTAAAATGCTAGCTGGAAATGGTCAGAAGGGTTGGCCCTATATCCAACGTTTGCTGGTCGACTTATTTCAATTCATGGAGCCATTTTTGAGGAATGCTGAACTAGGAGCTCCGGTTCATTTCCTATATAAAGGTACCCTTAGAGTGCTACTTGTGCTGCTCCATGATTTCCCCGAGTTCCTTTGTGATTATCACTTCACTTTCTGTGATGTTATTCCCCCAAGTTGCATTCAAATGCGAAATATCATACTCAGTGCGTTTCCCCGTAACATGAGGCTTCCTGACCCATCTACTCCGAATTTAAAGATTGATTTGCTTGCTGAAATCACTCAATCTCCACGAGTTCTTTCTGAGGTTGATGCAGCTTTGAAAGTAAAGCAGATGAAAACCGATGTGGATGAGTATCTCAAGACAAGGCAACAAGGCTCCTCATTTTTGACTGAATTGAAGCAAAAATTGCTCCTTTTACCAACCGAAGCTTCCTCAGCCGGTACTCGTTACAATGTACCCCTCATCAACTCCCTTGTACTCTATGTTGGGATGCAGGCTATCCAGCAGCTTCAGACTAGAACACCGCACGCACAGGCAAATCAAACTGGTCCCTTTGTGTATTTGGTAGGGGCTGCTTTGGATATCTTCCAGACTCTGATAGGGGACCTAGATACTGAAGGACGATACCTTTTCCTAAATGCGATAGCAAACCAACTGCGCTATCCAAACACCCATACACATTACTTCTCCTTCATTGTTCTTTACTTGTTCGCAGAGTCAAACCAGCAGGAAATTATCCAGGAGCAAATCACAAGGGTCTTGTTGGAACGTCTCATTGTTAATCGTCCACATCCATGGGGTCTACTGATCACATTCATTGAGCTAATCAAGAATCCGAGGTACAACTTCTGGAACCGAGCTTTCATTAGGTGTGCACCAGAGATTGAAAAACTCTTTGAATCTGTATCGAGGTCTTGTGGGGCAAAGCCTGTGGATGAGAGTATGGTTTCAGGTTGGGTACCTGAGACTGCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2400

Amino Acids

266.19

Weight (kDa)

5.52

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_TTP_bind PF16417 9 - 130 7.7e-50 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 273 - 490 1.4e-89 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 608 - 747 1.8e-48 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
ARM_Cnot1 PF25097 950 - 1125 3.4e-54 Cnot1 ARM repeat
NOT1_connector PF23590 977 - 1123 3.2e-18 CCR4-NOT transcription complex subunit 1, NOT1_connector
Not1 PF04054 1327 - 1686 3.4e-143 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3164
AarI CACCTGC 2 cut(s) 4837, 5596
Acc16I TGCGCA 3 cut(s) 2291, 2408, 4911
Acc36I ACCTGC 6 cut(s) 152, 1425, 2305, 2707, 4837, 5596
Acc65I GGTACC 2 cut(s) 6305, 7180
AccB1I GGYRCC 3 cut(s) 5631, 6305, 7180
AccB7I CCANNNNNTGG 3 cut(s) 3904, 4586, 5323
AccBSI CCGCTC 1 cut(s) 5179
AccI GTMKAC 3 cut(s) 1970, 6231, 7024
AccII CGCG 3 cut(s) 87, 2204, 3846
AccIII TCCGGA 1 cut(s) 973
AclI AACGTT 1 cut(s) 6220
AcoI YGGCCR 3 cut(s) 2366, 4074, 5841
AcuI CTGAAG 9 cut(s) 1197, 1392, 2034, 2375, 3621, 4133, 5732, 6716, 6858
AcvI CACGTG 1 cut(s) 3889
AcyI GRCGYC 1 cut(s) 63
AflII CTTAAG 2 cut(s) 1595, 3247
AflIII ACRYGT 3 cut(s) 3060, 4090, 4347
AgeI ACCGGT 1 cut(s) 5730
AhdI GACNNNNNGTC 1 cut(s) 6031
AjuI GAANNNNNNNTTGG 8 cut(s) 2012, 2044, 3891, 3923, 5187, 5219, 6634, 6666
AloI GAACNNNNNNTCC 2 cut(s) 6270, 6302
Alw21I GWGCWC 5 cut(s) 93, 1524, 2758, 6283, 7099
Alw26I GTCTC 9 cut(s) 851, 1825, 2608, 2645, 2868, 3949, 4532, 6998, 7181
Alw44I GTGCAC 1 cut(s) 7095
AlwNI CAGNNNCTG 7 cut(s) 2708, 2864, 4586, 4819, 4883, 5425, 6817
Ama87I CYCGRG 7 cut(s) 310, 848, 4757, 5539, 5600, 6143, 6344
Aor13HI TCCGGA 1 cut(s) 973
AoxI GGCC 9 cut(s) 1653, 2366, 2518, 4074, 5206, 5499, 5523, 5841, 6206
ApaLI GTGCAC 1 cut(s) 7095
ArsI GACNNNNNNTTYG 4 cut(s) 1952, 1984, 3927, 3959
AseI ATTAAT 3 cut(s) 1401, 5478, 5790
AsiGI ACCGGT 1 cut(s) 5730
Asp700I GAANNNNTTC 1 cut(s) 201
Asp718I GGTACC 2 cut(s) 6305, 7180
AspLEI GCGC 7 cut(s) 2292, 2409, 3963, 4912, 5090, 5824, 6882
AsuC2I CCSGG 4 cut(s) 1526, 4555, 5540, 5541
AsuII TTCGAA 1 cut(s) 3092
AsuNHI GCTAGC 1 cut(s) 6181
AvaI CYCGRG 7 cut(s) 310, 848, 4757, 5539, 5600, 6143, 6344
AvaII GGWCC 6 cut(s) 1776, 2071, 4583, 5728, 6770, 6826
AxyI CCTNAGG 1 cut(s) 2536
BaeGI GKGCMC 1 cut(s) 7099
BaeI ACNNNNGTAYC 4 cut(s) 1334, 1334, 1367, 1367
BalI TGGCCA 2 cut(s) 2368, 5843
BamHI GGATCC 2 cut(s) 5894, 6040
BanI GGYRCC 3 cut(s) 5631, 6305, 7180
BanII GRGCYC 4 cut(s) 93, 1089, 3256, 6283
BarI GAAGNNNNNNTAC 2 cut(s) 2482, 2514
BauI CACGAG 5 cut(s) 947, 1013, 2802, 3255, 6510
BbrPI CACGTG 1 cut(s) 3889
Bbv12I GWGCWC 5 cut(s) 93, 1524, 2758, 6283, 7099
BbvCI CCTCAGC 3 cut(s) 4871, 5814, 6655
BceAI ACGGC 1 cut(s) 2533
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciVI GTATCC 5 cut(s) 946, 1362, 1531, 2187, 4362
BclI TGATCA 3 cut(s) 3279, 3745, 7029
BcnI CCSGG 4 cut(s) 1526, 4555, 5540, 5541
BcoDI GTCTC 9 cut(s) 851, 1825, 2608, 2645, 2868, 3949, 4532, 6998, 7181
BfoI RGCGCY 1 cut(s) 5091
BfrI CTTAAG 2 cut(s) 1595, 3247
BfuAI ACCTGC 6 cut(s) 152, 1425, 2305, 2707, 4837, 5596
BfuI GTATCC 5 cut(s) 946, 1362, 1531, 2187, 4362
BglII AGATCT 2 cut(s) 453, 3417
BmcAI AGTACT 1 cut(s) 250
Bme18I GGWCC 6 cut(s) 1776, 2071, 4583, 5728, 6770, 6826
BmeRI GACNNNNNGTC 1 cut(s) 6031
BmeT110I CYCGRG 7 cut(s) 310, 848, 4757, 5539, 5600, 6143, 6344
BmrI ACTGGG 3 cut(s) 751, 2517, 2586
BmtI GCTAGC 1 cut(s) 6185
BmuI ACTGGG 3 cut(s) 751, 2517, 2586
BoxI GACNNNNGTC 1 cut(s) 1584
BplI GAGNNNNNCTC 8 cut(s) 293, 325, 1031, 1063, 1907, 1939, 4751, 4783
BpmI CTGGAG 5 cut(s) 476, 1706, 2585, 2877, 4821
Bpu10I CCTNAGC 3 cut(s) 4871, 5814, 6655
Bpu14I TTCGAA 1 cut(s) 3092
BpuEI CTTGAG 9 cut(s) 1367, 1730, 2627, 3434, 3506, 3747, 4132, 5864, 6563
BpuMI CCSGG 4 cut(s) 1526, 4555, 5540, 5541
Bsa29I ATCGAT 1 cut(s) 1340
BsaAI YACGTR 2 cut(s) 3126, 3889
BsaBI GATNNNNATC 4 cut(s) 803, 1944, 5705, 6573
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 2 cut(s) 851, 4532
BsaWI WCCGGW 3 cut(s) 973, 5730, 6283
Bse118I RCCGGY 4 cut(s) 4523, 5634, 5730, 6659
Bse21I CCTNAGG 1 cut(s) 2536
Bse3DI GCAATG 6 cut(s) 317, 590, 894, 1483, 2285, 5106
Bse8I GATNNNNATC 4 cut(s) 803, 1944, 5705, 6573
BseAI TCCGGA 1 cut(s) 973
BseCI ATCGAT 1 cut(s) 1340
BseJI GATNNNNATC 4 cut(s) 803, 1944, 5705, 6573
BseMI GCAATG 6 cut(s) 317, 590, 894, 1483, 2285, 5106
BseSI GKGCMC 1 cut(s) 7099
BsgI GTGCAG 3 cut(s) 817, 4248, 4748
Bsh1236I CGCG 3 cut(s) 87, 2204, 3846
Bsh1285I CGRYCG 2 cut(s) 1340, 3231
BshFI GGCC 9 cut(s) 1655, 2368, 2520, 4076, 5208, 5501, 5525, 5843, 6208
BshNI GGYRCC 3 cut(s) 5631, 6305, 7180
BshTI ACCGGT 1 cut(s) 5730
BshVI ATCGAT 1 cut(s) 1340
BsiEI CGRYCG 2 cut(s) 1340, 3231
BsiHKAI GWGCWC 5 cut(s) 93, 1524, 2758, 6283, 7099
BsiHKCI CYCGRG 7 cut(s) 310, 848, 4757, 5539, 5600, 6143, 6344
BslFI GGGAC 6 cut(s) 1789, 1850, 3778, 4396, 6756, 6839
BsmAI GTCTC 9 cut(s) 851, 1825, 2608, 2645, 2868, 3949, 4532, 6998, 7181
BsmBI CGTCTC 3 cut(s) 2645, 2868, 6998
BsmFI GGGAC 6 cut(s) 1789, 1850, 3778, 4396, 6756, 6839
BsmI GAATGC 5 cut(s) 1043, 4051, 5068, 6271, 6399
BsnI GGCC 9 cut(s) 1655, 2368, 2520, 4076, 5208, 5501, 5525, 5843, 6208
Bso31I GGTCTC 2 cut(s) 851, 4532
BsoBI CYCGRG 7 cut(s) 310, 848, 4757, 5539, 5600, 6143, 6344
Bsp119I TTCGAA 1 cut(s) 3092
Bsp1286I GDGCHC 7 cut(s) 93, 1089, 1524, 2758, 3256, 6283, 7099
Bsp13I TCCGGA 1 cut(s) 973
Bsp1407I TGTACA 1 cut(s) 2104
Bsp19I CCATGG 3 cut(s) 2328, 4017, 7016
BspANI GGCC 9 cut(s) 1655, 2368, 2520, 4076, 5208, 5501, 5525, 5843, 6208
BspDI ATCGAT 1 cut(s) 1340
BspEI TCCGGA 1 cut(s) 973
BspFNI CGCG 3 cut(s) 87, 2204, 3846
BspHI TCATGA 1 cut(s) 3055
BspMAI CTGCAG 6 cut(s) 555, 2302, 2506, 2704, 5421, 5578
BspMI ACCTGC 6 cut(s) 152, 1425, 2305, 2707, 4837, 5596
BspOI GCTAGC 1 cut(s) 6185
BspT104I TTCGAA 1 cut(s) 3092
BspT107I GGYRCC 3 cut(s) 5631, 6305, 7180
BspTI CTTAAG 2 cut(s) 1595, 3247
BspTNI GGTCTC 2 cut(s) 851, 4532
BsrBI CCGCTC 1 cut(s) 5179
BsrDI GCAATG 6 cut(s) 317, 590, 894, 1483, 2285, 5106
BsrFI RCCGGY 4 cut(s) 4523, 5634, 5730, 6659
BsrGI TGTACA 1 cut(s) 2104
BssAI RCCGGY 4 cut(s) 4523, 5634, 5730, 6659
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 5 cut(s) 947, 1013, 2802, 3255, 6510
BssT1I CCWWGG 8 cut(s) 1949, 2328, 3002, 4017, 4926, 5022, 6028, 7016
Bst2BI CACGAG 5 cut(s) 947, 1013, 2802, 3255, 6510
Bst6I CTCTTC 5 cut(s) 463, 1362, 3522, 4152, 5547
BstACI GRCGYC 1 cut(s) 63
BstAFI CTTAAG 2 cut(s) 1595, 3247
BstAPI GCANNNNNTGC 4 cut(s) 1265, 2753, 4489, 6628
BstAUI TGTACA 1 cut(s) 2104
BstBAI YACGTR 2 cut(s) 3126, 3889
BstBI TTCGAA 1 cut(s) 3092
BstDSI CCRYGG 3 cut(s) 2328, 4017, 7016
BstENI CCTNNNNNAGG 2 cut(s) 1904, 2818
BstFNI CGCG 3 cut(s) 87, 2204, 3846
BstH2I RGCGCY 1 cut(s) 5091
BstHHI GCGC 7 cut(s) 2292, 2409, 3963, 4912, 5090, 5824, 6882
BstMAI GTCTC 9 cut(s) 851, 1825, 2608, 2645, 2868, 3949, 4532, 6998, 7181
BstMCI CGRYCG 2 cut(s) 1340, 3231
BstPAI GACNNNNGTC 1 cut(s) 1584
BstSLI GKGCMC 1 cut(s) 7099
BstUI CGCG 3 cut(s) 87, 2204, 3846
BstX2I RGATCY 9 cut(s) 453, 2476, 2558, 3417, 4216, 5402, 5584, 5894, 6040
BstXI CCANNNNNNTGG 6 cut(s) 653, 1811, 2335, 3384, 5926, 7017
BstYI RGATCY 9 cut(s) 453, 2476, 2558, 3417, 4216, 5402, 5584, 5894, 6040
Bsu15I ATCGAT 1 cut(s) 1340
Bsu36I CCTNAGG 1 cut(s) 2536
BsuI GTATCC 5 cut(s) 946, 1362, 1531, 2187, 4362
BsuRI GGCC 9 cut(s) 1655, 2368, 2520, 4076, 5208, 5501, 5525, 5843, 6208
BsuTUI ATCGAT 1 cut(s) 1340
BtgI CCRYGG 3 cut(s) 2328, 4017, 7016
BtgZI GCGATG 3 cut(s) 2705, 4147, 4701
BtsI GCAGTG 7 cut(s) 21, 1233, 3970, 4817, 5224, 5414, 5801
BveI ACCTGC 6 cut(s) 152, 1425, 2305, 2707, 4837, 5596
CaiI CAGNNNCTG 7 cut(s) 2708, 2864, 4586, 4819, 4883, 5425, 6817
CciI TCATGA 1 cut(s) 3055
CfoI GCGC 7 cut(s) 2292, 2409, 3963, 4912, 5090, 5824, 6882
Cfr10I RCCGGY 4 cut(s) 4523, 5634, 5730, 6659
Cfr9I CCCGGG 1 cut(s) 5539
ClaI ATCGAT 1 cut(s) 1340
CpoI CGGWCCG 1 cut(s) 5728
CseI GACGC 4 cut(s) 71, 2342, 2593, 2885
CsiI ACCWGGT 3 cut(s) 2819, 4399, 5590
CspAI ACCGGT 1 cut(s) 5730
CspCI CAANNNNNGTGG 2 cut(s) 7134, 7169
CspI CGGWCCG 1 cut(s) 5728
DraI TTTAAA 1 cut(s) 6474
DriI GACNNNNNGTC 1 cut(s) 6031
EaeI YGGCCR 3 cut(s) 2366, 4074, 5841
Eam1104I CTCTTC 5 cut(s) 463, 1362, 3522, 4152, 5547
Eam1105I GACNNNNNGTC 1 cut(s) 6031
EarI CTCTTC 5 cut(s) 463, 1362, 3522, 4152, 5547
EciI GGCGGA 1 cut(s) 4379
Ecl136II GAGCTC 2 cut(s) 91, 6281
Eco130I CCWWGG 8 cut(s) 1949, 2328, 3002, 4017, 4926, 5022, 6028, 7016
Eco24I GRGCYC 4 cut(s) 93, 1089, 3256, 6283
Eco31I GGTCTC 2 cut(s) 851, 4532
Eco32I GATATC 2 cut(s) 2397, 6805
Eco47I GGWCC 6 cut(s) 1776, 2071, 4583, 5728, 6770, 6826
Eco53kI GAGCTC 2 cut(s) 91, 6281
Eco57I CTGAAG 9 cut(s) 1197, 1392, 2034, 2375, 3621, 4133, 5732, 6716, 6858
Eco72I CACGTG 1 cut(s) 3889
Eco81I CCTNAGG 1 cut(s) 2536
Eco88I CYCGRG 7 cut(s) 310, 848, 4757, 5539, 5600, 6143, 6344
EcoICRI GAGCTC 2 cut(s) 91, 6281
EcoNI CCTNNNNNAGG 2 cut(s) 1904, 2818
EcoO109I RGGNCCY 4 cut(s) 1776, 4583, 5524, 6826
EcoRV GATATC 2 cut(s) 2397, 6805
EcoT14I CCWWGG 8 cut(s) 1949, 2328, 3002, 4017, 4926, 5022, 6028, 7016
EcoT22I ATGCAT 3 cut(s) 1270, 2287, 5068
EcoT38I GRGCYC 4 cut(s) 93, 1089, 3256, 6283
ErhI CCWWGG 8 cut(s) 1949, 2328, 3002, 4017, 4926, 5022, 6028, 7016
Esp3I CGTCTC 3 cut(s) 2645, 2868, 6998
FalI AAGNNNNNCTT 6 cut(s) 1365, 1397, 4094, 4126, 6294, 6326
FaqI GGGAC 6 cut(s) 1789, 1850, 3778, 4396, 6756, 6839
FauI CCCGC 1 cut(s) 3851
FauNDI CATATG 2 cut(s) 3292, 4540
FbaI TGATCA 3 cut(s) 3279, 3745, 7029
FblI GTMKAC 3 cut(s) 1970, 6231, 7024
FriOI GRGCYC 4 cut(s) 93, 1089, 3256, 6283
FspI TGCGCA 3 cut(s) 2291, 2408, 4911
GlaI GCGC 7 cut(s) 2291, 2408, 3962, 4911, 5089, 5823, 6881
GsuI CTGGAG 5 cut(s) 476, 1706, 2585, 2877, 4821
HaeII RGCGCY 1 cut(s) 5091
HaeIII GGCC 9 cut(s) 1655, 2368, 2520, 4076, 5208, 5501, 5525, 5843, 6208
HgaI GACGC 4 cut(s) 71, 2342, 2593, 2885
HhaI GCGC 7 cut(s) 2292, 2409, 3963, 4912, 5090, 5824, 6882
Hin1I GRCGYC 1 cut(s) 63
Hin6I GCGC 7 cut(s) 2290, 2407, 3961, 4910, 5088, 5822, 6880
HinP1I GCGC 7 cut(s) 2290, 2407, 3961, 4910, 5088, 5822, 6880
HincII GTYRAC 4 cut(s) 448, 2055, 6013, 6232
HindII GTYRAC 4 cut(s) 448, 2055, 6013, 6232
HindIII AAGCTT 4 cut(s) 3725, 3777, 5129, 6648
HpaI GTTAAC 1 cut(s) 6013
Hpy99I CGWCG 1 cut(s) 65
HpyCH4IV ACGT 9 cut(s) 633, 866, 3125, 3654, 3707, 3888, 4843, 6220, 6991
HpySE526I ACGT 9 cut(s) 633, 866, 3125, 3654, 3707, 3888, 4843, 6220, 6991
Hsp92I GRCGYC 1 cut(s) 63
HspAI GCGC 7 cut(s) 2290, 2407, 3961, 4910, 5088, 5822, 6880
KflI GGGWCCC 1 cut(s) 1776
Kpn2I TCCGGA 1 cut(s) 973
KpnI GGTACC 2 cut(s) 6309, 7184
Ksp22I TGATCA 3 cut(s) 3279, 3745, 7029
KspAI GTTAAC 1 cut(s) 6013
MabI ACCWGGT 3 cut(s) 2819, 4399, 5590
MaeII ACGT 9 cut(s) 633, 866, 3125, 3654, 3707, 3888, 4843, 6220, 6991
MbiI CCGCTC 1 cut(s) 5179
MfeI CAATTG 3 cut(s) 825, 3912, 4269
MflI RGATCY 9 cut(s) 453, 2476, 2558, 3417, 4216, 5402, 5584, 5894, 6040
MhlI GDGCHC 7 cut(s) 93, 1089, 1524, 2758, 3256, 6283, 7099
MlsI TGGCCA 2 cut(s) 2368, 5843
MluNI TGGCCA 2 cut(s) 2368, 5843
Mox20I TGGCCA 2 cut(s) 2368, 5843
Mph1103I ATGCAT 3 cut(s) 1270, 2287, 5068
MroI TCCGGA 1 cut(s) 973
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 2 cut(s) 2368, 5843
MslI CAYNNNNRTG 8 cut(s) 651, 757, 1273, 1619, 1812, 4656, 6404, 7015
Msp20I TGGCCA 2 cut(s) 2368, 5843
MspA1I CMGCKG 6 cut(s) 496, 556, 1210, 2864, 4745, 5425
MspCI CTTAAG 2 cut(s) 1595, 3247
MunI CAATTG 3 cut(s) 825, 3912, 4269
Mva1269I GAATGC 5 cut(s) 1043, 4051, 5068, 6271, 6399
MvnI CGCG 3 cut(s) 87, 2204, 3846
NciI CCSGG 4 cut(s) 1526, 4555, 5540, 5541
NcoI CCATGG 3 cut(s) 2328, 4017, 7016
NdeI CATATG 2 cut(s) 3292, 4540
NheI GCTAGC 1 cut(s) 6181
NmuCI GTSAC 4 cut(s) 1235, 1301, 5412, 5932
NsbI TGCGCA 3 cut(s) 2291, 2408, 4911
NsiI ATGCAT 3 cut(s) 1270, 2287, 5068
NspV TTCGAA 1 cut(s) 3092
PaeI GCATGC 1 cut(s) 1259
PaeR7I CTCGAG 3 cut(s) 310, 848, 6143
PagI TCATGA 1 cut(s) 3055
PaqCI CACCTGC 2 cut(s) 4837, 5596
PciI ACATGT 3 cut(s) 3060, 4090, 4347
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 2800
PctI GAATGC 5 cut(s) 1043, 4051, 5068, 6271, 6399
PdmI GAANNNNTTC 1 cut(s) 201
PflMI CCANNNNNTGG 3 cut(s) 3904, 4586, 5323
PfoI TCCNGGA 1 cut(s) 6960
PinAI ACCGGT 1 cut(s) 5730
Ple19I CGATCG 2 cut(s) 1340, 3231
PmaCI CACGTG 1 cut(s) 3889
PmlI CACGTG 1 cut(s) 3889
Ppu21I YACGTR 2 cut(s) 3126, 3889
PpuMI RGGWCCY 3 cut(s) 1776, 4583, 6826
PscI ACATGT 3 cut(s) 3060, 4090, 4347
PshAI GACNNNNGTC 1 cut(s) 1584
PshBI ATTAAT 3 cut(s) 1401, 5478, 5790
PsiI TTATAA 1 cut(s) 3164
Psp124BI GAGCTC 2 cut(s) 93, 6283
Psp1406I AACGTT 1 cut(s) 6220
Psp5II RGGWCCY 3 cut(s) 1776, 4583, 6826
PspCI CACGTG 1 cut(s) 3889
PspPPI RGGWCCY 3 cut(s) 1776, 4583, 6826
PspXI VCTCGAGB 1 cut(s) 6143
PstI CTGCAG 6 cut(s) 555, 2302, 2506, 2704, 5421, 5578
PstNI CAGNNNCTG 7 cut(s) 2708, 2864, 4586, 4819, 4883, 5425, 6817
PsuI RGATCY 9 cut(s) 453, 2476, 2558, 3417, 4216, 5402, 5584, 5894, 6040
PvuI CGATCG 2 cut(s) 1340, 3231
PvuII CAGCTG 4 cut(s) 556, 1210, 2864, 5425
RseI CAYNNNNRTG 8 cut(s) 651, 757, 1273, 1619, 1812, 4656, 6404, 7015
Rsr2I CGGWCCG 1 cut(s) 5728
RsrII CGGWCCG 1 cut(s) 5728
SacI GAGCTC 2 cut(s) 93, 6283
SalI GTCGAC 1 cut(s) 6230
ScaI AGTACT 1 cut(s) 250
SduI GDGCHC 7 cut(s) 93, 1089, 1524, 2758, 3256, 6283, 7099
SexAI ACCWGGT 3 cut(s) 2819, 4399, 5590
Sfr274I CTCGAG 3 cut(s) 310, 848, 6143
SfuI TTCGAA 1 cut(s) 3092
SinI GGWCC 6 cut(s) 1776, 2071, 4583, 5728, 6770, 6826
SlaI CTCGAG 3 cut(s) 310, 848, 6143
SmaI CCCGGG 1 cut(s) 5541
SmiMI CAYNNNNRTG 8 cut(s) 651, 757, 1273, 1619, 1812, 4656, 6404, 7015
SphI GCATGC 1 cut(s) 1259
SspI AATATT 3 cut(s) 2950, 4041, 4125
SstI GAGCTC 2 cut(s) 93, 6283
StyI CCWWGG 8 cut(s) 1949, 2328, 3002, 4017, 4926, 5022, 6028, 7016
TaiI ACGT 9 cut(s) 636, 869, 3128, 3657, 3710, 3891, 4846, 6223, 6994
TatI WGTACW 5 cut(s) 248, 1294, 2104, 5241, 6697
TauI GCSGC 2 cut(s) 1655, 2204
TseFI GTSAC 4 cut(s) 1235, 1301, 5412, 5932
Tsp45I GTSAC 4 cut(s) 1235, 1301, 5412, 5932
TspGWI ACGGA 3 cut(s) 1321, 1600, 5741
TspMI CCCGGG 1 cut(s) 5539
Van91I CCANNNNNTGG 3 cut(s) 3904, 4586, 5323
Vha464I CTTAAG 2 cut(s) 1595, 3247
VneI GTGCAC 1 cut(s) 7095
VpaK11BI GGWCC 6 cut(s) 1776, 2071, 4583, 5728, 6770, 6826
VspI ATTAAT 3 cut(s) 1401, 5478, 5790
XagI CCTNNNNNAGG 2 cut(s) 1904, 2818
XbaI TCTAGA 2 cut(s) 1279, 4177
XcmI CCANNNNNNNNNTGG 5 cut(s) 3381, 3866, 4210, 4967, 5081
XhoI CTCGAG 3 cut(s) 310, 848, 6143
XmaI CCCGGG 1 cut(s) 5539
XmiI GTMKAC 3 cut(s) 1970, 6231, 7024
XmnI GAANNNNTTC 1 cut(s) 201
ZrmI AGTACT 1 cut(s) 250
Zsp2I ATGCAT 3 cut(s) 1270, 2287, 5068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.