Rorug01G0197100

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005282
Physical Location & Seq
Reverse (-)
27619980 .. 27652809
32830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0197100.1

Sequence Viewer

Length: 1341 bp
ATGAGAGAGATGGAGATTAGAGAAGATCACCATGAAGACGATACAACAAGAGCACTCTATGAGGTAGCAGTTGCAGGCTCATTATCATCTTTCAAAAGATTGATTCAGAAAGACCCTCTCATCCTCAACAAAGTTTCACTCACTCACTTCAGTGAAACTCCCCTCCACATCTCAGCTCTGCTCGGCCACCTTGATTTCACCAAGGCTATTCTTACTCATAACTCGAGACTTGCCACCGTGAGGGACTCCTTGAGACGCCCGCCGCTCCTCTTGGCATCTGCCGAGGGCCATAAGGACACTGTTCAAGCTCTTCTGAAAGCCTACCCCGACGCTTGCTTGTTTCGGGATCAGGATGGAAGAATCCCTCTTCACTATGCCGCTATGAGAGGACACGCTGAGGTCTTAGCTGAGTTGATGCGCACAAAACCCGAATCAGTCATGTGGAGGGTTCTTAATAGAGGAGACACGGTTTTCCACTTATGTGTTACTTATAACCAATTAGAATGCTTGAAGCTATTGGTGGAAGAGGTAGGAGACAATAGAGACCTCCTCAATGCTAGAGCTGGATGCGATGGTGGTATGACCATCCTCGAATTAGCTGTGATGCTAAGGCAAATTGAGACAATAAAATACTTGCTCTCGCTGCCTGTTGTACGAGAAGAAGCAAAGAAAGATGGAAAAAAGGGAAGGAAAAGTGAAAATAAGGTGAATGGGAAATGCTGTAACAGTGGAACTTGGGTGGAAGAGAAACGCGGCATGCTGATGGTTGTGGCTACCATGATCTCAACAATGACATTCCAAGCTACAATTAACCCACCAGGTGGGTTCTGGCAAGAAATGAACACCAATTCGACTTTTGATGGAGCAACTATTTGCAGTGCAACAAAGCCTTGTGTTGCCGGAACTGCAGTCTCAAGCTACATACATAGAGATTACTTCAACAACTTCCAGACTTACAATGCCATATGCTTCCTTTTTTCTTTGAGCATCACTCTTTTGCTTATAAGCGGATTTCCTCTCCGTAAACGAGTACTTGTCTGGCTCTTGTCAATAGTCATGTGTCTCACTCTCACATTCTTGGCGCTGACCTTCAGTGAAGGTGCAAAGATGGTAGTACCCAAAAGTGTTAATTCTGAATATACTACCGCTATCAGGATTGTGAGTTCTGTCACATTGTTTTGGGTTCCGCTACTTGGACTGGTTGGTTTATATAACGTTATTCGACTTCTCATTTGGCTCGTAAAGCTGTTGTGGCGCATAATCTTTCCCAAAAAGGAGGTGACGATCAACGACAATTTTCCGCCTCCCATAAGCCCACATGACAGCAGTTCGAACGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

446

Amino Acids

50.09

Weight (kDa)

8.81

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 22 - 108 1e-08 Ankyrin repeats (3 copies)
Ank_4 PF13637 91 - 135 6.7e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 114 - 176 7.5e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 153 - 214 3.6e-07 Ankyrin repeats (3 copies)
PGG PF13962 246 - 368 1.2e-17 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 492, 1004
Acc16I TGCGCA 1 cut(s) 419
AccB7I CCANNNNNTGG 1 cut(s) 821
AccBSI CCGCTC 1 cut(s) 265
AccII CGCG 1 cut(s) 753
AciI CCGC 8 cut(s) 260, 263, 378, 753, 1008, 1146, 1187, 1301
AclI AACGTT 1 cut(s) 1215
AclWI GGATC 1 cut(s) 354
AcoI YGGCCR 1 cut(s) 184
AcuI CTGAAG 2 cut(s) 133, 1075
AcyI GRCGYC 1 cut(s) 256
AdeI CACNNNGTG 1 cut(s) 821
AfaI GTAC 3 cut(s) 654, 1032, 1116
AfiI CCNNNNNNNGG 4 cut(s) 240, 821, 1152, 1193
AgsI TTSAA 4 cut(s) 94, 305, 511, 940
AjnI CCWGG 1 cut(s) 817
AleI CACNNNNGTG 2 cut(s) 150, 480
AloI GAACNNNNNNTCC 2 cut(s) 1147, 1179
AluBI AGCT 9 cut(s) 176, 308, 407, 514, 563, 599, 803, 918, 1246
AluI AGCT 9 cut(s) 176, 308, 407, 514, 563, 599, 803, 918, 1246
Alw21I GWGCWC 1 cut(s) 55
Alw26I GTCTC 8 cut(s) 220, 247, 456, 528, 537, 614, 916, 1067
AlwI GGATC 1 cut(s) 354
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 2 cut(s) 184, 286
ApeKI GCWGC 1 cut(s) 643
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
AspLEI GCGC 3 cut(s) 420, 1084, 1257
AspS9I GGNCC 1 cut(s) 286
AsuHPI GGTGA 4 cut(s) 20, 190, 718, 1291
AsuII TTCGAA 1 cut(s) 1331
AvaI CYCGRG 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 55
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 630
BccI CCATC 8 cut(s) 4, 347, 566, 593, 668, 757, 854, 1102
BciT130I CCWGG 1 cut(s) 819
BcoDI GTCTC 8 cut(s) 220, 247, 456, 528, 537, 614, 916, 1067
BfaI CTAG 1 cut(s) 558
BfmI CTRYAG 1 cut(s) 906
BfoI RGCGCY 1 cut(s) 1085
BisI GCNGC 4 cut(s) 263, 378, 644, 754
BlsI GCNGC 4 cut(s) 264, 379, 645, 755
BmcAI AGTACT 1 cut(s) 1032
Bme1390I CCNGG 1 cut(s) 819
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 1185
BmrFI CCNGG 1 cut(s) 819
BmsI GCATC 5 cut(s) 284, 405, 557, 594, 996
BpiI GAAGAC 1 cut(s) 42
BplI GAGNNNNNCTC 4 cut(s) 534, 566, 976, 1008
Bpu10I CCTNAGC 2 cut(s) 396, 608
Bpu14I TTCGAA 1 cut(s) 1331
BpuEI CTTGAG 2 cut(s) 271, 898
BsaHI GRCGYC 1 cut(s) 256
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 2 cut(s) 201, 282
BsaXI ACNNNNNCTCC 2 cut(s) 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 240, 821, 1152, 1193
Bse1I ACTGG 1 cut(s) 1203
BseBI CCWGG 1 cut(s) 819
BseDI CCNNGG 2 cut(s) 201, 282
BseGI GGATG 4 cut(s) 120, 358, 572, 585
BseLI CCNNNNNNNGG 4 cut(s) 240, 821, 1152, 1193
BseMII CTCAG 3 cut(s) 186, 387, 399
BseNI ACTGG 1 cut(s) 1203
BseRI GAGGAG 3 cut(s) 257, 474, 539
BseXI GCAGC 1 cut(s) 630
Bsh1236I CGCG 1 cut(s) 753
BshFI GGCC 2 cut(s) 186, 288
BsiHKAI GWGCWC 1 cut(s) 55
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 900
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 4 cut(s) 240, 821, 1152, 1193
BsmAI GTCTC 8 cut(s) 220, 247, 456, 528, 537, 614, 916, 1067
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 2 cut(s) 186, 288
Bso31I GGTCTC 1 cut(s) 537
BsoBI CYCGRG 1 cut(s) 223
Bsp119I TTCGAA 1 cut(s) 1331
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 4 cut(s) 25, 346, 780, 1284
BspACI CCGC 8 cut(s) 260, 263, 378, 753, 1008, 1146, 1187, 1301
BspANI GGCC 2 cut(s) 186, 288
BspCNI CTCAG 3 cut(s) 185, 388, 400
BspFNI CGCG 1 cut(s) 753
BspLI GGNNCC 1 cut(s) 1185
BspMAI CTGCAG 1 cut(s) 910
BspPI GGATC 1 cut(s) 354
BspQI GCTCTTC 1 cut(s) 315
BspT104I TTCGAA 1 cut(s) 1331
BspTNI GGTCTC 1 cut(s) 537
BsrBI CCGCTC 1 cut(s) 265
BsrI ACTGG 1 cut(s) 1203
BssECI CCNNGG 2 cut(s) 201, 282
BssMI GATC 4 cut(s) 25, 346, 780, 1284
BssNI GRCGYC 1 cut(s) 256
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 819
Bst4CI ACNGT 4 cut(s) 238, 301, 469, 728
Bst6I CTCTTC 4 cut(s) 315, 372, 519, 738
BstACI GRCGYC 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 1331
BstC8I GCNNGC 4 cut(s) 76, 260, 334, 758
BstDEI CTNAG 5 cut(s) 172, 396, 403, 408, 608
BstF5I GGATG 4 cut(s) 120, 358, 572, 585
BstFNI CGCG 1 cut(s) 753
BstH2I RGCGCY 1 cut(s) 1085
BstHHI GCGC 3 cut(s) 420, 1084, 1257
BstKTI GATC 4 cut(s) 28, 349, 783, 1287
BstMAI GTCTC 8 cut(s) 220, 247, 456, 528, 537, 614, 916, 1067
BstMBI GATC 4 cut(s) 25, 346, 780, 1284
BstMWI GCNNNNNNNGC 4 cut(s) 643, 905, 1243, 1252
BstNI CCWGG 1 cut(s) 819
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 1 cut(s) 817
BstSFI CTRYAG 1 cut(s) 906
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 1 cut(s) 630
BstV2I GAAGAC 1 cut(s) 42
BsuRI GGCC 2 cut(s) 186, 288
BtgZI GCGATG 1 cut(s) 585
BtsCI GGATG 4 cut(s) 120, 358, 572, 585
BtsI GCAGTG 1 cut(s) 883
BtsIMutI CAGTG 5 cut(s) 157, 297, 733, 883, 1099
Cac8I GCNNGC 4 cut(s) 76, 260, 334, 758
CfoI GCGC 3 cut(s) 420, 1084, 1257
Cfr13I GGNCC 1 cut(s) 286
CseI GACGC 2 cut(s) 264, 338
CsiI ACCWGGT 1 cut(s) 817
Csp6I GTAC 3 cut(s) 653, 1031, 1115
CviAII CATG 6 cut(s) 32, 439, 757, 778, 1057, 1319
CviQI GTAC 3 cut(s) 653, 1031, 1115
DdeI CTNAG 5 cut(s) 172, 396, 403, 408, 608
DpnI GATC 4 cut(s) 27, 348, 782, 1286
DpnII GATC 4 cut(s) 25, 346, 780, 1284
DraIII CACNNNGTG 1 cut(s) 821
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 4 cut(s) 315, 372, 519, 738
EarI CTCTTC 4 cut(s) 315, 372, 519, 738
EciI GGCGGA 1 cut(s) 1290
Eco130I CCWWGG 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 537
Eco57I CTGAAG 2 cut(s) 133, 1075
Eco88I CYCGRG 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 817
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 6 cut(s) 35, 442, 760, 781, 1060, 1322
FaqI GGGAC 1 cut(s) 257
FatI CATG 6 cut(s) 31, 438, 756, 777, 1056, 1318
FauI CCCGC 1 cut(s) 267
FauNDI CATATG 1 cut(s) 965
Fnu4HI GCNGC 4 cut(s) 263, 378, 644, 754
FokI GGATG 4 cut(s) 107, 365, 572, 579
Fsp4HI GCNGC 4 cut(s) 263, 378, 644, 754
FspAI RTGCGCAY 1 cut(s) 419
FspBI CTAG 1 cut(s) 558
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 3 cut(s) 419, 1083, 1256
GluI GCNGC 4 cut(s) 263, 378, 644, 754
HaeII RGCGCY 1 cut(s) 1085
HaeIII GGCC 2 cut(s) 186, 288
HapII CCGG 1 cut(s) 900
HgaI GACGC 2 cut(s) 264, 338
HhaI GCGC 3 cut(s) 420, 1084, 1257
Hin1I GRCGYC 1 cut(s) 256
Hin1II CATG 6 cut(s) 35, 442, 760, 781, 1060, 1322
Hin6I GCGC 3 cut(s) 418, 1082, 1255
HinP1I GCGC 3 cut(s) 418, 1082, 1255
HinfI GANTC 4 cut(s) 103, 245, 360, 431
HpaII CCGG 1 cut(s) 900
HphI GGTGA 4 cut(s) 20, 190, 718, 1291
Hpy166II GTNNAC 1 cut(s) 1025
Hpy188I TCNGA 3 cut(s) 108, 315, 1135
Hpy188III TCNNGA 5 cut(s) 225, 344, 350, 949, 1153
Hpy8I GTNNAC 1 cut(s) 1025
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 3 cut(s) 681, 1091, 1099
HpyCH4III ACNGT 4 cut(s) 238, 301, 469, 728
HpyCH4IV ACGT 2 cut(s) 1215, 1335
HpyCH4V TGCA 5 cut(s) 74, 876, 881, 908, 1103
HpyF10VI GCNNNNNNNGC 4 cut(s) 643, 905, 1243, 1252
HpyF3I CTNAG 5 cut(s) 172, 396, 403, 408, 608
HpySE526I ACGT 2 cut(s) 1215, 1335
Hsp92I GRCGYC 1 cut(s) 256
Hsp92II CATG 6 cut(s) 35, 442, 760, 781, 1060, 1322
HspAI GCGC 3 cut(s) 418, 1082, 1255
Kzo9I GATC 4 cut(s) 25, 346, 780, 1284
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 270, 863
Lsp1109I GCAGC 1 cut(s) 630
LweI GCATC 5 cut(s) 284, 405, 557, 594, 996
MabI ACCWGGT 1 cut(s) 817
MaeI CTAG 1 cut(s) 558
MaeII ACGT 2 cut(s) 1215, 1335
MaeIII GTNAC 4 cut(s) 484, 722, 1168, 1279
MalI GATC 4 cut(s) 27, 348, 782, 1286
MbiI CCGCTC 1 cut(s) 265
MboI GATC 4 cut(s) 25, 346, 780, 1284
MboII GAAGA 8 cut(s) 35, 47, 302, 359, 369, 536, 671, 755
MhlI GDGCHC 1 cut(s) 55
MluCI AATT 7 cut(s) 497, 593, 615, 807, 847, 1129, 1294
MlyI GAGTC 1 cut(s) 239
MseI TTAA 3 cut(s) 453, 810, 1128
MslI CAYNNNNRTG 3 cut(s) 150, 480, 761
MspI CCGG 1 cut(s) 900
MspR9I CCNGG 1 cut(s) 819
Mva1269I GAATGC 1 cut(s) 509
MvaI CCWGG 1 cut(s) 819
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 4 cut(s) 643, 905, 1243, 1252
NdeI CATATG 1 cut(s) 965
NdeII GATC 4 cut(s) 25, 346, 780, 1284
NlaIII CATG 6 cut(s) 35, 442, 760, 781, 1060, 1322
NlaIV GGNNCC 1 cut(s) 1185
NmeAIII GCCGAG 2 cut(s) 162, 307
NmuCI GTSAC 2 cut(s) 1168, 1279
NsbI TGCGCA 1 cut(s) 419
NspI RCATGY 1 cut(s) 760
NspV TTCGAA 1 cut(s) 1331
OliI CACNNNNGTG 2 cut(s) 150, 480
PaeI GCATGC 1 cut(s) 760
PaeR7I CTCGAG 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 315
PctI GAATGC 1 cut(s) 509
PfeI GAWTC 3 cut(s) 103, 360, 431
PflMI CCANNNNNTGG 1 cut(s) 821
PkrI GCNGC 4 cut(s) 264, 379, 645, 755
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PsiI TTATAA 2 cut(s) 492, 1004
Psp1406I AACGTT 1 cut(s) 1215
Psp6I CCWGG 1 cut(s) 817
PspGI CCWGG 1 cut(s) 817
PspN4I GGNNCC 1 cut(s) 1185
PspPI GGNCC 1 cut(s) 286
PstI CTGCAG 1 cut(s) 910
RsaI GTAC 3 cut(s) 654, 1032, 1116
RsaNI GTAC 3 cut(s) 653, 1031, 1115
RseI CAYNNNNRTG 3 cut(s) 150, 480, 761
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 3 cut(s) 453, 810, 1128
SatI GCNGC 4 cut(s) 263, 378, 644, 754
Sau3AI GATC 4 cut(s) 25, 346, 780, 1284
Sau96I GGNCC 1 cut(s) 286
ScaI AGTACT 1 cut(s) 1032
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 819
SduI GDGCHC 1 cut(s) 55
SexAI ACCWGGT 1 cut(s) 817
SfaNI GCATC 5 cut(s) 284, 405, 557, 594, 996
SfcI CTRYAG 1 cut(s) 906
Sfr274I CTCGAG 1 cut(s) 223
SfuI TTCGAA 1 cut(s) 1331
SlaI CTCGAG 1 cut(s) 223
SmiMI CAYNNNNRTG 3 cut(s) 150, 480, 761
SmlI CTYRAG 3 cut(s) 223, 250, 913
SmoI CTYRAG 3 cut(s) 223, 250, 913
SphI GCATGC 1 cut(s) 760
Sse9I AATT 7 cut(s) 497, 593, 615, 807, 847, 1129, 1294
SsiI CCGC 8 cut(s) 260, 263, 378, 753, 1008, 1146, 1187, 1301
SspMI CTAG 1 cut(s) 558
StyD4I CCNGG 1 cut(s) 817
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 4 cut(s) 238, 301, 469, 728
TaiI ACGT 2 cut(s) 1218, 1338
TaqI TCGA 5 cut(s) 224, 591, 851, 1222, 1331
TasI AATT 7 cut(s) 497, 593, 615, 807, 847, 1129, 1294
TatI WGTACW 1 cut(s) 1030
TauI GCSGC 3 cut(s) 265, 380, 756
TfiI GAWTC 3 cut(s) 103, 360, 431
Tru1I TTAA 3 cut(s) 453, 810, 1128
Tru9I TTAA 3 cut(s) 453, 810, 1128
TscAI CASTG 5 cut(s) 157, 304, 733, 883, 1099
TseFI GTSAC 2 cut(s) 1168, 1279
TseI GCWGC 1 cut(s) 643
Tsp45I GTSAC 2 cut(s) 1168, 1279
TspDTI ATGAA 2 cut(s) 48, 854
TspGWI ACGGA 1 cut(s) 1010
TspRI CASTG 5 cut(s) 157, 304, 733, 883, 1099
Van91I CCANNNNNTGG 1 cut(s) 821
XceI RCATGY 1 cut(s) 760
XcmI CCANNNNNNNNNTGG 1 cut(s) 825
XhoI CTCGAG 1 cut(s) 223
XspI CTAG 1 cut(s) 558
ZrmI AGTACT 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.