Rroxscaffold_4G00306720

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005282
Physical Location & Seq
Reverse (-)
27673334 .. 27693308
19975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306720.1

Sequence Viewer

Length: 6843 bp
ATGCTCAAGTTTTCGCAGACCACTGCGAACCAGATTCGGTTCCTCCTGCAAAGCCTCAACGACGCCAACTCGGACTCTGTTCACCGAGAGCTTACTCAGTCTGCTGTTTCATGCCAGTTTATTGAATATGGAGTTGAGGGAAGCGTTTTGCTGCTCCAAACCTGCCTGGATCATCTGAATCATTACAGAACAGATACAAAGAATGTGGCACTGGAACGGGTTCTTGCCTCAATTTTCAAATATCTCATCGACCAACCAAATTTCAGTACTATGTTTTGTGAATCACTAAGAAATACAGAGGTCAATGAAGGAATTGTGGAGAATTTCTCGAGTGTATTGCAACTGTCTGTACCTGAGAAAATTGGCATTGGTCTTGCTTTGTCAGATTCAGAAAACCTAGACACCAGAATATGTGGCAAAAACTTCTGTATGGCTCAGATAGAGAAATTGTGTGAAACTCCTGTTGACAGGATCTCTTCTGAGAAAATTCAGAATATTGTTATGTTCCTTCAGCGGTCTGAGGGTCTTTCCAAGCATGTAGATTCCTTTATGCAGATATTATCTTTACTGCAGCTGAAAGATGATGAAAACTCTTTTGTTTTAAGTCCATTGCTTTCAGATGAGCTTCGTGATGCCAATTTTTTGAGGAACATGGACTTGTTCCGTGAAAGCGGAGAAAATGATTTTGACGCTATTTTAGCAGAAATGGAGAAGGAGATGAGTATGGGAGATATTATGAAGGAATTAGGTTATGGATGCACAGTTGATTCATCTCAGTGCAAAGAGATTTTATCCCTTTTCTTACCACTCAATGAGATTACTATCTCCAAAATACTTGGCACGATTGCCTGCACCCATGCTGGTCTTGAGGATAATCAGAACACATTTTCAACCTTCCGGTTGGCGCTTGGTTATAGTACTTCATCTGATTTGCCTATGCTAAACACCTGGAACATTGATGTCCTTGTGGATACAATCAATCAACTTGCTCCAGACACCAACTGGATAAGTGTTATTGAAAATTTGGATCATGAGGGGTTCTACATTCCTAATCAGGAGGCATTCTCTTTTTTCATGTCTGTATATCAGCATGTGTGTCAGGAGCCATTCCCCCTACATGCAATCTGTGGGTCTGTTTGGAAGAATACCGAGGGACAGCTATCTTTTCTCAAACATGCTGTTTCATCACCACCAGAAGTATTTAATTTTGCTCACTCTGTTAGGCAGCTGCCCTACACGGATACGGTGAATGGCCATAAGCTACAACTTGGACATGCAAATCATGCATGGTTGTGTCTAGACCTTTTGGATGTACTTTGTCACCTAGCAGAGAGGGGTCATGCTCTTTCTGTTCGATCGATACTTGAGTATCCTCTTCAACACTGTCCTGAAGTCCTGCTTCTTGGGATGGCACACATTAATACTCCATACAACCTGCTGCAATATGAAGTGTCTTTCATTGTTTTCCCCATGATAGTAAAAAATGTCATGGGTAATGGCATGATTGTTCACCTCTGGCATACTAACCCCTCTTTGGTGCTGCGGGGATTTGTGGATGCCTACAATAGCGATCCAGACAGCATGACTAGAATATTGGATATCTGTCAAGAGCTAAAGATTTTGTCGTCCGTCATGGAGATGATTCCTTCTCCCTTTAGTATCAGATTGGCGGCCCTTGCTTTCAGAAAAGAGCTTGTAGAACTCGAGAAGTGGTTGAGTAGTAATTTGAATACATATAAAGATACTTTCTTTGAGGAGTGCCTCAAGTTCCTGAAGGAGATTCAAGCTGGAGGGACCCAAGATTTCTCAACCAGTCCTTTCCAACATTCTGGTGCTGTCTCAACTCTCTATGGGGACACTACTGCTACATTCTCGAAGGTTCTTAAAGCTCATGTCGCCCAGATTACTTCTAGCCACCTTACTGAGGAGATGGAAAAACTCTCTCTATCAATTATGGATTCTAATCCAAGGCTGCAGAATGGTGGGCCTACAGAGTCATCAACAGATGGATATGCAGATGACATTGAAGCTGAAGCCAATTCTTACTTCCATCAAATGTTTTCTGGTCAGTTGACTATTGAATCAATGGTCCAAATGCTTGCCCGTTTCAAAGAATCTTCTGTTCAAAGGGAACAATCCATTTTTGAGTGCATGATTGCTAATCTGTTTGAAGAGTACAGATTCTTTCCGAAATATCCTGAAAGACAGCTCAAAATTGCCGCAATTTTATTTGGGTCTGTCATCAAGCAGCAGCTTGTAACCCATCTGACTCTAGGGATTGCTCTGCGTGGTGTTTTAGATGCATTGCGCAAACCTGCGGATTCAAAAATGTTTGTGTTTGGAACAAAGGCTCTTGAGCAGTTTGTGGAGCGTCTGATTGAATGGCCACAGTACTGCAATCACATTCTTCAGATATCCCATTTGCGCAATACTCATTCAGAGCTGGTGGTATTCATTGAACAGGCTCTAGCCAGGATTTCATCCGGTCATTCAGATTCAGATGGAAGTAACCATGCTTCTGCCGTTCATCATCACGGTCCCAGTCAAGTTACCTCAGGAACTGTGGAGTTAAATGGATCAAGCATCTTACAAACTGGGCAGACACTATCTTCTCCGCTCCAGCTCCATCAGAGACATGAAAGCTCTTTTGATGACCGTCACAAGTCCTCAACTCCATCTAATGAGGTAAAGCCACTTTCATCTTCTGCAACGCAACCTGCAGTTGTTCCTGTTAGTGACGCTCCGAGTATTCAGAAGTTACAAAATGCAGTTAGTGCTCCATCAATGTTGTCTTCTGCTTCCCCTGGTTTTGTTCGTCCGTCACGAGGAGCTACCTCTACTAGGTTTGGCTCTGCTTTGAGCATTGAAACATTGGTTGCAGCAGCTGAGAAAAGAGATGCTCCCGTTGAGGCTCCAGCAGAGATTCAGGACAAAGTATTATTTATAGTTAATAATATATCACTTGCTAATATTGAAGTTAAGTCGAAGGAGTTTTCTGAGATACTGAAAGAGCAATACTATCCCTGGTTTGCGCAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCCAATTTTCATGACTTGTACCTGAAATTTCTGGACAAACTTAATTCGAAGGCTTTGAATAAAGAGATTGTTCAAGCCACGTATGAGAACTGCAAGGTTCTTCTAGGGTCTGAGCTTATAAAATCAAGCTCAGAGGAGCGTTCATTGCTAAAAAATTTAGGTAGCTGGCTTGGGAAGCTTACTATTGGAAGGAATCAAGTCTTACGGGCTCGTGAAATTGATCCCAAATCTTTGATCATAGAGGCATATGAGAAAGGGTTGATGATTGCTGTCATACCTTTTACTTCAAAGATTTTGGAACCATGCCAGAATAGCCTCGCGTATCAACCACCGAATCCCTGGACAATGGGTATTCTTGGATTACTTGCTGAGATCTATTCCATGCCAAACTTGAAGATGAACCTCAAGTTTGATATAGAGATTGCTGAAGTTAAATCTGGAATTATTTCTCCACTTAACCAAGTTGAGCTACCACTTGACGTTGCTCCATCTAATTCTGGGAGCCATACACATCTGTTATCTCAGTATGCTACCTTGATGGAGGATGACAAGCTTGCTTTGGGGTTATCTGATCAGATCACTTCTGCTCAAGGACTGTTACAAGCTACTCCATCACAGTCCCCATTTTCTGTTAGTCAGCATCCCACAGCAATACCTAACATTGGAACCCACGTAATTATCAACCAGAAGCTCAGTGCCTTGGGGGGCTGCACAGTGGTTCCAATTGCTATGGATAGAGCTATCAAAGAAATTGTCTCTGGTATTGTTCAGCGCAGTGTTTCTATAGCTACCCAGACAACTAAGGAGCTTGTTTTAAAGGACTATGCCATGGAATCAGATGACACTAGAATTTTTACTGCAGCGCACTTGATGGTTGCCAGTTTGGCAGGAAGTCTAGCGCATGTCACATGCAAGGAACCCTTGCGCAGTTCAATATTAAGTCAGCTAAGAAATTCTCTTCAGGGATTAAACATCGCAAGTGAACTTCTAGAACAAGCTGTTCAACTTGTAACCAATGATAATCTGGATCTTGGCTGTGCAGTCATTGAGCAGGCTGCTACAGATAAGGCAGTAGCAACAATTGACGGGGAAATAGGTCCGCAGCTTTCAGTAAGAAGAAAGAGAGAAGGTGTTGGTGCAACCTATTTTGATGCAAACATGTATCCACAAGGTTCTATGGGTGTTGTGCCAGAAGCTCTCCGCCCTAAACCTGGTCATTTATCCCTTTCTCAGCAACGAGTTTATGAGGATTTTGTTCGGCTTCCCTGGCAAAACCAATCTATTCAAAGTTCGCACGTTCTGCCAGTTGCTACCACAGCTTCATCCGGTAGTGCTGGTCTCACTAGCACATATGGTTCAGCATCCGGACAACTTAACACGGGCTACTCACCAGGACCTGGAAGCAAATTTGAAGCTGTTTCTCGTCCATTGGATGAAGCGATGGAATCCAATTCTGCCCTCCATCTTAGTGCTTCATCTATTCATGTTGGGGCGGCTGATGGTGTTTCCCAGCATAGTTCTGAAAATGATTCTGTTGTTGGCTCATTTCCTTCTGCTGCTTCTGCCCCTGAGCTGCACTCTGTAGAGTCATCTGATGCTGTAAAGGAATCTGGAGTGTCCTCACAGCCACCACCTTCACCTGCTGTAACTGAACGTCTTGGAAGTCTCTCAGAACCTTCCCTCACCACAGGGGCTGCATTGGAAAAATACCAGATTGTTGCACAGAAGTTGGAAGCTCTGGTGACTAACGATGCTAGAGAATCAGAAATCCAGGGAGTCATTGGTGAAGTGCCTGATATTATACTCAGATGTGTTAGTCGAGATGAGGCCGCCTTGGCTGTGGCTCAAAAGGTTTTCAAGGGTTTGTACGAGAATGCAACGAATCAGATTCATGTTGGCGCACATCTAGCAATGCTGACTGCGATTCGTGATGTTTGCAAGCTTGTCTTTACACAGCTCACTAGTTGGGTGATTTATTCTGATGAGGAGCGGAAGTTTAATAAAGAAATAACTGTTGGCCTTATACGTAGTGAACTGCTAAACCTTGCAGACTACAATGTTCACATGGCAAAACTTATTGATGGTGGCAGGAATAAGCCAGCAACAGAGTTCGCCACTTCCTTGCTCCAAACTTTGGTGAATGAAGAATCTAAAGTTATCTCAGAACTTCAGAGTCTTGTTGATGCAATGGGAAAGGCTGCTGCAAAGCCTGGATCTCCCGAGTCACTGCAGCAGGTGGTTGAAACTGTCAAGAATCCTGCTAATGGAGCTGCTCCATCTGGTGTTAATGTTGGGAAGGATGACAAGGCCAGACAATCGAGAGAGAAAAAGGCTAGTGCTTCTGTTCACTCCCCAGTAAGCAGGGAAGAGTTCAGTATTGTGGAGTCTGCAGAGCCAGATCCTCCTGGTTTTCGCGAGCAGGTGTCTATGCTGTTTGCTGAGTGGTACCGGCTTTGTGAACTTCCTGGTGCAAACGAAAATAGTTGTGGCCATTTAATCATACAGTTGCACTCAAGTGGCATGCTGAAGGGGGATGATCTTACGGACCGATTTTTCCGACTACTCACAGAGCTTACTGTTGCACATTGTCTATCTTCAGAGGTGATTAATTCAGGCACACTGCAAACTCCTCAACAAGTGCAGAATCTATCCTTCCTTGCCATTGATATTTATGCTAAACTTATGGAGCAGGGATCAAGTAAACTAATTCTCTTGTCCAAGATTTTGGCAGTTACAGTGAGATTCATTCAAAAGGATGCAGAGGAGAAGAAATCATCTTTCAATGCAAGACCGTATTTTAGATTGTTTGTTAACTGGTTGCTAGACCTTGGGTCTTTGGATCCTGTTATTGATGGTGCTAACTTTCAGATATTGACAGCCTTTGCAAATGCATTTCATGCGTTGCAACCCCTCAAAGTTCCTACATTCAGGTTCACTTCCTGTATAAAGGCACCCTTAGAGTGTTACTTGTGCTGCTTCATGATTTCCCCGAGTTCCTTTGTGATTATCACTTCACTTTCTGTGATGTTATTCCCCCGAGTTGCATTCAAATGCGAAATATCATCCTCAGTGCATTTCCCCATTGATTTGCTTGCTGAAATCACTCAATCTCCACGGGTTCTTTCTGAGGTTGATGCAGCTCTGAAATTAAAGCAGATGAAGACCGATGTGGATGAGTATCTCAAGACAAGGCAACAAGGTTCTTCATTTTTGACCGAATTGAAGCAAAAATTGCTCCTTTTACCAACCGAAGCTGCCTCAGCTGGTACTCGTTACAATGTACCCCTCATCAACTCCCTTGTACTCTATGTGGGGATGCAGGCTATCCAGCAGCTTCAGGCTAGAACGCCTCATGCACAGGCAAATCAAACTGTTCCATTTGTATATTTGGTGGGTGCTGCTTTGGATATCTTCCAGACTCTGATAGTGGACCTAGATACTGAAGGACGCTACCTTTTCCTAAATGCAATTGCAAACCAACTGCGCTATCCGAACACCCATACACATTACTTCTCCTTCATTGTTCTTTACTTGTTCGCGGAATCAAACCAGCAGGAAATTATCCAGGAGCAAATCACAAGGGTATTGTTGGAACGTCTCATTGTTAATCGCCCTCATCCATGGGGTCTACTGATAACATTCATTGAGCTAATCAAGAACCCGAGGTACAACTTCTGGAACCGGGCTTTCATAAGGTGTGCACCAGAGATTGAAAAACTCTTTGAATCTGTATCAAGGTCTTGTGGCGCAAAGCCTGTGGATGAGAGTATGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2280

Amino Acids

251.88

Weight (kDa)

5.48

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 488 - 629 3.7e-34 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 664 - 826 2.3e-67 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 970 - 1163 1.4e-80 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1261 - 1396 1.2e-46 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
ARM_Cnot1 PF25097 1598 - 1774 1.1e-51 Cnot1 ARM repeat
NOT1_connector PF23590 1622 - 1772 1.1e-14 CCR4-NOT transcription complex subunit 1, NOT1_connector
Not1 PF04054 2049 - 2266 1.2e-71 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3185
AarI CACCTGC 3 cut(s) 4714, 5290, 5476
Acc16I TGCGCA 4 cut(s) 2309, 2426, 3033, 3992
Acc36I ACCTGC 7 cut(s) 170, 1443, 2323, 2725, 4714, 5290, 5476
Acc65I GGTACC 1 cut(s) 5511
AccB1I GGYRCC 2 cut(s) 5511, 6016
AccB7I CCANNNNNTGG 2 cut(s) 4463, 5200
AccBSI CCGCTC 2 cut(s) 2617, 5056
AccI GTMKAC 1 cut(s) 6694
AccII CGCG 3 cut(s) 3386, 5481, 6605
AccIII TCCGGA 1 cut(s) 4430
AcoI YGGCCR 3 cut(s) 1252, 2384, 5554
AcsI RAATTY 9 cut(s) 259, 322, 486, 1021, 3092, 3220, 3915, 4018, 4472
AcyI GRCGYC 1 cut(s) 63
AfiI CCNNNNNNNGG 9 cut(s) 1534, 1924, 2825, 2841, 3728, 4277, 4463, 5200, 5330
AflIII ACRYGT 1 cut(s) 4224
AhdI GACNNNNNGTC 1 cut(s) 5896
AhlI ACTAGT 1 cut(s) 5027
AjuI GAANNNNNNNTTGG 4 cut(s) 2030, 2062, 5064, 5096
AleI CACNNNNGTG 1 cut(s) 5580
Alw21I GWGCWC 2 cut(s) 2779, 6769
Alw26I GTCTC 6 cut(s) 1843, 2626, 3826, 4409, 4736, 6668
Alw44I GTGCAC 1 cut(s) 6765
AlwNI CAGNNNCTG 5 cut(s) 2561, 2885, 4463, 4760, 6487
Ama87I CYCGRG 6 cut(s) 328, 1703, 5285, 6055, 6102, 6727
Aor13HI TCCGGA 1 cut(s) 4430
AoxI GGCC 8 cut(s) 1252, 1671, 1985, 2384, 4893, 5083, 5373, 5554
ApaLI GTGCAC 1 cut(s) 6765
ApoI RAATTY 9 cut(s) 259, 322, 486, 1021, 3092, 3220, 3915, 4018, 4472
ArsI GACNNNNNNTTYG 2 cut(s) 3804, 3836
AseI ATTAAT 2 cut(s) 1419, 5673
Asp700I GAANNNNTTC 2 cut(s) 219, 3511
Asp718I GGTACC 1 cut(s) 5511
AspS9I GGNCC 9 cut(s) 1672, 1794, 1985, 2089, 2537, 4163, 4460, 5611, 6496
AsuC2I CCSGG 1 cut(s) 6749
AsuII TTCGAA 1 cut(s) 3113
AvaI CYCGRG 6 cut(s) 328, 1703, 5285, 6055, 6102, 6727
AvaII GGWCC 7 cut(s) 1794, 2089, 2537, 4163, 4460, 5611, 6496
AxyI CCTNAGG 1 cut(s) 2554
BaeGI GKGCMC 1 cut(s) 6769
BaeI ACNNNNGTAYC 4 cut(s) 1352, 1352, 1385, 1385
BalI TGGCCA 3 cut(s) 1254, 2386, 5556
BamHI GGATCC 1 cut(s) 5905
BanI GGYRCC 2 cut(s) 5511, 6016
BanII GRGCYC 2 cut(s) 3069, 3277
BarI GAAGNNNNNNTAC 2 cut(s) 2500, 2532
BauI CACGAG 2 cut(s) 2823, 3276
BbsI GAAGAC 2 cut(s) 2784, 6233
Bbv12I GWGCWC 2 cut(s) 2779, 6769
BbvCI CCTCAGC 1 cut(s) 6325
BceAI ACGGC 1 cut(s) 2507
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciVI GTATCC 4 cut(s) 964, 1234, 1380, 4239
BclI TGATCA 2 cut(s) 3300, 3637
BcnI CCSGG 1 cut(s) 6749
BcoDI GTCTC 6 cut(s) 1843, 2626, 3826, 4409, 4736, 6668
BcuI ACTAGT 1 cut(s) 5027
BfoI RGCGCY 1 cut(s) 908
BfuAI ACCTGC 7 cut(s) 170, 1443, 2323, 2725, 4714, 5290, 5476
BfuI GTATCC 4 cut(s) 964, 1234, 1380, 4239
BglI GCCNNNNNGGC 2 cut(s) 3950, 4901
BglII AGATCT 1 cut(s) 3438
BmcAI AGTACT 3 cut(s) 268, 919, 2393
Bme18I GGWCC 7 cut(s) 1794, 2089, 2537, 4163, 4460, 5611, 6496
BmeRI GACNNNNNGTC 1 cut(s) 5896
BmeT110I CYCGRG 6 cut(s) 328, 1703, 5285, 6055, 6102, 6727
BmgT120I GGNCC 9 cut(s) 1672, 1794, 1985, 2089, 2537, 4163, 4460, 5611, 6496
BmrI ACTGGG 3 cut(s) 2535, 2604, 5414
BmuI ACTGGG 3 cut(s) 2535, 2604, 5414
BpiI GAAGAC 2 cut(s) 2784, 6233
BplI GAGNNNNNCTC 6 cut(s) 311, 343, 1049, 1081, 4628, 4660
BpmI CTGGAG 5 cut(s) 975, 1809, 2603, 2898, 4698
Bpu10I CCTNAGC 2 cut(s) 4635, 6325
Bpu14I TTCGAA 1 cut(s) 3113
BpuEI CTTGAG 8 cut(s) 887, 1385, 1748, 2375, 3455, 3639, 5563, 6233
BpuMI CCSGG 1 cut(s) 6749
Bsa29I ATCGAT 1 cut(s) 1358
BsaAI YACGTR 3 cut(s) 3147, 3739, 5093
BsaBI GATNNNNATC 3 cut(s) 821, 1962, 6243
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 4409
BsaWI WCCGGW 4 cut(s) 897, 2483, 4391, 4430
BsaXI ACNNNNNCTCC 4 cut(s) 5045, 5075, 6160, 6190
Bsc4I CCNNNNNNNGG 9 cut(s) 1534, 1924, 2825, 2841, 3728, 4277, 4463, 5200, 5330
Bse118I RCCGGY 1 cut(s) 5514
Bse21I CCTNAGG 1 cut(s) 2554
Bse3DI GCAATG 5 cut(s) 608, 2303, 3209, 4983, 5259
Bse8I GATNNNNATC 3 cut(s) 821, 1962, 6243
BseAI TCCGGA 1 cut(s) 4430
BseCI ATCGAT 1 cut(s) 1358
BseJI GATNNNNATC 3 cut(s) 821, 1962, 6243
BseLI CCNNNNNNNGG 9 cut(s) 1534, 1924, 2825, 2841, 3728, 4277, 4463, 5200, 5330
BseMI GCAATG 5 cut(s) 608, 2303, 3209, 4983, 5259
BseRI GAGGAG 7 cut(s) 1769, 1940, 2841, 3215, 5066, 5685, 5843
BseSI GKGCMC 1 cut(s) 6769
BseYI CCCAGC 1 cut(s) 4575
BsgI GTGCAG 5 cut(s) 835, 3760, 4125, 4625, 5726
Bsh1236I CGCG 3 cut(s) 3386, 5481, 6605
Bsh1285I CGRYCG 1 cut(s) 1358
BshFI GGCC 8 cut(s) 1254, 1673, 1987, 2386, 4895, 5085, 5375, 5556
BshNI GGYRCC 2 cut(s) 5511, 6016
BshVI ATCGAT 1 cut(s) 1358
BsiEI CGRYCG 1 cut(s) 1358
BsiHKAI GWGCWC 2 cut(s) 2779, 6769
BsiHKCI CYCGRG 6 cut(s) 328, 1703, 5285, 6055, 6102, 6727
BsiSI CCGG 6 cut(s) 898, 2484, 4392, 4431, 5515, 6748
BslFI GGGAC 5 cut(s) 1167, 1807, 1868, 2523, 3670
BslI CCNNNNNNNGG 9 cut(s) 1534, 1924, 2825, 2841, 3728, 4277, 4463, 5200, 5330
BsmAI GTCTC 6 cut(s) 1843, 2626, 3826, 4409, 4736, 6668
BsmBI CGTCTC 1 cut(s) 6668
BsmFI GGGAC 5 cut(s) 1167, 1807, 1868, 2523, 3670
BsmI GAATGC 3 cut(s) 1061, 4945, 6110
BsnI GGCC 8 cut(s) 1254, 1673, 1987, 2386, 4895, 5085, 5375, 5556
Bso31I GGTCTC 1 cut(s) 4409
BsoBI CYCGRG 6 cut(s) 328, 1703, 5285, 6055, 6102, 6727
Bsp119I TTCGAA 1 cut(s) 3113
Bsp1286I GDGCHC 4 cut(s) 2779, 3069, 3277, 6769
Bsp13I TCCGGA 1 cut(s) 4430
Bsp19I CCATGG 2 cut(s) 3894, 6686
Bsp68I TCGCGA 1 cut(s) 5481
BspANI GGCC 8 cut(s) 1254, 1673, 1987, 2386, 4895, 5085, 5375, 5556
BspDI ATCGAT 1 cut(s) 1358
BspEI TCCGGA 1 cut(s) 4430
BspFNI CGCG 3 cut(s) 3386, 5481, 6605
BspHI TCATGA 3 cut(s) 1030, 3076, 6045
BspMAI CTGCAG 6 cut(s) 573, 1977, 2722, 3928, 5298, 5458
BspMI ACCTGC 7 cut(s) 170, 1443, 2323, 2725, 4714, 5290, 5476
BspT104I TTCGAA 1 cut(s) 3113
BspT107I GGYRCC 2 cut(s) 5511, 6016
BspTNI GGTCTC 1 cut(s) 4409
BsrBI CCGCTC 2 cut(s) 2617, 5056
BsrDI GCAATG 5 cut(s) 608, 2303, 3209, 4983, 5259
BsrFI RCCGGY 1 cut(s) 5514
BssAI RCCGGY 1 cut(s) 5514
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 2 cut(s) 2823, 3276
BssT1I CCWWGG 6 cut(s) 1967, 3765, 3894, 4899, 5893, 6686
Bst2BI CACGAG 2 cut(s) 2823, 3276
Bst6I CTCTTC 5 cut(s) 481, 1380, 2166, 4029, 5427
BstACI GRCGYC 1 cut(s) 63
BstAPI GCANNNNNTGC 5 cut(s) 1283, 2774, 4366, 5963, 6298
BstBAI YACGTR 3 cut(s) 3147, 3739, 5093
BstBI TTCGAA 1 cut(s) 3113
BstDSI CCRYGG 3 cut(s) 3894, 6179, 6686
BstENI CCTNNNNNAGG 2 cut(s) 1922, 2839
BstFNI CGCG 3 cut(s) 3386, 5481, 6605
BstH2I RGCGCY 1 cut(s) 908
BstMAI GTCTC 6 cut(s) 1843, 2626, 3826, 4409, 4736, 6668
BstMCI CGRYCG 1 cut(s) 1358
BstNSI RCATGY 9 cut(s) 539, 1094, 1121, 1178, 1277, 3971, 3978, 4228, 5590
BstSLI GKGCMC 1 cut(s) 6769
BstSNI TACGTA 1 cut(s) 5093
BstUI CGCG 3 cut(s) 3386, 5481, 6605
BstV2I GAAGAC 2 cut(s) 2784, 6233
BstX2I RGATCY 6 cut(s) 471, 3438, 4093, 5279, 5464, 5905
BstXI CCANNNNNNTGG 2 cut(s) 1829, 5791
BstYI RGATCY 6 cut(s) 471, 3438, 4093, 5279, 5464, 5905
Bsu15I ATCGAT 1 cut(s) 1358
Bsu36I CCTNAGG 1 cut(s) 2554
BsuI GTATCC 4 cut(s) 964, 1234, 1380, 4239
BsuRI GGCC 8 cut(s) 1254, 1673, 1987, 2386, 4895, 5085, 5375, 5556
BsuTUI ATCGAT 1 cut(s) 1358
BtgI CCRYGG 3 cut(s) 3894, 6179, 6686
BtgZI GCGATG 2 cut(s) 4024, 4520
BtsI GCAGTG 4 cut(s) 21, 3847, 5291, 5684
BtuMI TCGCGA 1 cut(s) 5481
BveI ACCTGC 7 cut(s) 170, 1443, 2323, 2725, 4714, 5290, 5476
CaiI CAGNNNCTG 5 cut(s) 2561, 2885, 4463, 4760, 6487
CciI TCATGA 3 cut(s) 1030, 3076, 6045
Cfr10I RCCGGY 1 cut(s) 5514
Cfr13I GGNCC 9 cut(s) 1672, 1794, 1985, 2089, 2537, 4163, 4460, 5611, 6496
ClaI ATCGAT 1 cut(s) 1358
CpoI CGGWCCG 1 cut(s) 5611
CseI GACGC 5 cut(s) 71, 698, 2360, 2747, 6522
CsiI ACCWGGT 1 cut(s) 4276
CspCI CAANNNNNGTGG 4 cut(s) 186, 221, 6804, 6839
CspI CGGWCCG 1 cut(s) 5611
DraI TTTAAA 1 cut(s) 3882
DriI GACNNNNNGTC 1 cut(s) 5896
EaeI YGGCCR 3 cut(s) 1252, 2384, 5554
Eam1104I CTCTTC 5 cut(s) 481, 1380, 2166, 4029, 5427
Eam1105I GACNNNNNGTC 1 cut(s) 5896
EarI CTCTTC 5 cut(s) 481, 1380, 2166, 4029, 5427
EciI GGCGGA 1 cut(s) 4256
Eco105I TACGTA 1 cut(s) 5093
Eco130I CCWWGG 6 cut(s) 1967, 3765, 3894, 4899, 5893, 6686
Eco24I GRGCYC 2 cut(s) 3069, 3277
Eco31I GGTCTC 1 cut(s) 4409
Eco32I GATATC 3 cut(s) 1600, 2415, 6475
Eco47I GGWCC 7 cut(s) 1794, 2089, 2537, 4163, 4460, 5611, 6496
Eco81I CCTNAGG 1 cut(s) 2554
Eco88I CYCGRG 6 cut(s) 328, 1703, 5285, 6055, 6102, 6727
EcoNI CCTNNNNNAGG 2 cut(s) 1922, 2839
EcoO109I RGGNCCY 2 cut(s) 1794, 4460
EcoRV GATATC 3 cut(s) 1600, 2415, 6475
EcoT14I CCWWGG 6 cut(s) 1967, 3765, 3894, 4899, 5893, 6686
EcoT22I ATGCAT 3 cut(s) 1288, 2305, 5959
EcoT38I GRGCYC 2 cut(s) 3069, 3277
ErhI CCWWGG 6 cut(s) 1967, 3765, 3894, 4899, 5893, 6686
Esp3I CGTCTC 1 cut(s) 6668
FalI AAGNNNNNCTT 8 cut(s) 1383, 1415, 3971, 4003, 4997, 5029, 6005, 6037
FaqI GGGAC 5 cut(s) 1167, 1807, 1868, 2523, 3670
FauI CCCGC 1 cut(s) 1536
FauNDI CATATG 2 cut(s) 3313, 4417
FbaI TGATCA 2 cut(s) 3300, 3637
FblI GTMKAC 1 cut(s) 6694
FriOI GRGCYC 2 cut(s) 3069, 3277
FspI TGCGCA 4 cut(s) 2309, 2426, 3033, 3992
GsaI CCCAGC 1 cut(s) 4579
GsuI CTGGAG 5 cut(s) 975, 1809, 2603, 2898, 4698
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 8 cut(s) 1254, 1673, 1987, 2386, 4895, 5085, 5375, 5556
HapII CCGG 6 cut(s) 898, 2484, 4392, 4431, 5515, 6748
HgaI GACGC 5 cut(s) 71, 698, 2360, 2747, 6522
Hin1I GRCGYC 1 cut(s) 63
HincII GTYRAC 3 cut(s) 466, 2073, 5878
HindII GTYRAC 3 cut(s) 466, 2073, 5878
HindIII AAGCTT 3 cut(s) 3242, 3617, 5006
HpaI GTTAAC 1 cut(s) 5878
HpaII CCGG 6 cut(s) 898, 2484, 4392, 4431, 5515, 6748
Hpy99I CGWCG 1 cut(s) 65
HpyCH4IV ACGT 7 cut(s) 3146, 3546, 3738, 4362, 4720, 5092, 6661
HpySE526I ACGT 7 cut(s) 3146, 3546, 3738, 4362, 4720, 5092, 6661
Hsp92I GRCGYC 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 1794
Kpn2I TCCGGA 1 cut(s) 4430
KpnI GGTACC 1 cut(s) 5515
Ksp22I TGATCA 2 cut(s) 3300, 3637
KspAI GTTAAC 1 cut(s) 5878
MabI ACCWGGT 1 cut(s) 4276
MaeII ACGT 7 cut(s) 3146, 3546, 3738, 4362, 4720, 5092, 6661
MbiI CCGCTC 2 cut(s) 2617, 5056
MfeI CAATTG 3 cut(s) 3789, 4146, 6534
MflI RGATCY 6 cut(s) 471, 3438, 4093, 5279, 5464, 5905
MhlI GDGCHC 4 cut(s) 2779, 3069, 3277, 6769
MlsI TGGCCA 3 cut(s) 1254, 2386, 5556
MluNI TGGCCA 3 cut(s) 1254, 2386, 5556
MlyI GAGTC 9 cut(s) 68, 2003, 2263, 4661, 4851, 5248, 5297, 5459, 6478
MmeI TCCRAC 4 cut(s) 1846, 4776, 5647, 6636
Mox20I TGGCCA 3 cut(s) 1254, 2386, 5556
Mph1103I ATGCAT 3 cut(s) 1288, 2305, 5959
MroI TCCGGA 1 cut(s) 4430
MroXI GAANNNNTTC 2 cut(s) 219, 3511
MscI TGGCCA 3 cut(s) 1254, 2386, 5556
MslI CAYNNNNRTG 7 cut(s) 775, 1291, 1637, 1830, 4533, 5580, 6115
Msp20I TGGCCA 3 cut(s) 1254, 2386, 5556
MspA1I CMGCKG 5 cut(s) 514, 574, 1228, 2885, 6329
MspI CCGG 6 cut(s) 898, 2484, 4392, 4431, 5515, 6748
MunI CAATTG 3 cut(s) 3789, 4146, 6534
Mva1269I GAATGC 3 cut(s) 1061, 4945, 6110
MvnI CGCG 3 cut(s) 3386, 5481, 6605
NciI CCSGG 1 cut(s) 6749
NcoI CCATGG 2 cut(s) 3894, 6686
NdeI CATATG 2 cut(s) 3313, 4417
NmuCI GTSAC 7 cut(s) 1319, 2657, 2735, 2820, 3970, 4807, 5289
NruI TCGCGA 1 cut(s) 5481
NsbI TGCGCA 4 cut(s) 2309, 2426, 3033, 3992
NsiI ATGCAT 3 cut(s) 1288, 2305, 5959
NspI RCATGY 9 cut(s) 539, 1094, 1121, 1178, 1277, 3971, 3978, 4228, 5590
NspV TTCGAA 1 cut(s) 3113
OliI CACNNNNGTG 1 cut(s) 5580
PaeI GCATGC 1 cut(s) 5590
PaeR7I CTCGAG 2 cut(s) 328, 1703
PagI TCATGA 3 cut(s) 1030, 3076, 6045
PaqCI CACCTGC 3 cut(s) 4714, 5290, 5476
PciI ACATGT 1 cut(s) 4224
PcsI WCGNNNNNNNCGW 1 cut(s) 2821
PctI GAATGC 3 cut(s) 1061, 4945, 6110
PdmI GAANNNNTTC 2 cut(s) 219, 3511
PflMI CCANNNNNTGG 2 cut(s) 4463, 5200
PfoI TCCNGGA 1 cut(s) 6630
Ple19I CGATCG 1 cut(s) 1358
PleI GAGTC 9 cut(s) 68, 2002, 2263, 4660, 4850, 5247, 5296, 5458, 6478
PpsI GAGTC 9 cut(s) 68, 2002, 2263, 4660, 4850, 5247, 5296, 5458, 6478
Ppu21I YACGTR 3 cut(s) 3147, 3739, 5093
PpuMI RGGWCCY 2 cut(s) 1794, 4460
PscI ACATGT 1 cut(s) 4224
PshBI ATTAAT 2 cut(s) 1419, 5673
PsiI TTATAA 1 cut(s) 3185
Psp5II RGGWCCY 2 cut(s) 1794, 4460
PspFI CCCAGC 1 cut(s) 4575
PspPI GGNCC 9 cut(s) 1672, 1794, 1985, 2089, 2537, 4163, 4460, 5611, 6496
PspPPI RGGWCCY 2 cut(s) 1794, 4460
PsrI GAACNNNNNNTAC 2 cut(s) 6716, 6748
PstI CTGCAG 6 cut(s) 573, 1977, 2722, 3928, 5298, 5458
PstNI CAGNNNCTG 5 cut(s) 2561, 2885, 4463, 4760, 6487
PsuI RGATCY 6 cut(s) 471, 3438, 4093, 5279, 5464, 5905
PvuI CGATCG 1 cut(s) 1358
PvuII CAGCTG 4 cut(s) 574, 1228, 2885, 6329
RruI TCGCGA 1 cut(s) 5481
RseI CAYNNNNRTG 7 cut(s) 775, 1291, 1637, 1830, 4533, 5580, 6115
Rsr2I CGGWCCG 1 cut(s) 5611
RsrII CGGWCCG 1 cut(s) 5611
Sau96I GGNCC 9 cut(s) 1672, 1794, 1985, 2089, 2537, 4163, 4460, 5611, 6496
ScaI AGTACT 3 cut(s) 268, 919, 2393
SchI GAGTC 9 cut(s) 68, 2003, 2263, 4661, 4851, 5248, 5297, 5459, 6478
SduI GDGCHC 4 cut(s) 2779, 3069, 3277, 6769
SexAI ACCWGGT 1 cut(s) 4276
Sfr274I CTCGAG 2 cut(s) 328, 1703
SfuI TTCGAA 1 cut(s) 3113
SinI GGWCC 7 cut(s) 1794, 2089, 2537, 4163, 4460, 5611, 6496
SlaI CTCGAG 2 cut(s) 328, 1703
SmiMI CAYNNNNRTG 7 cut(s) 775, 1291, 1637, 1830, 4533, 5580, 6115
SnaBI TACGTA 1 cut(s) 5093
SpeI ACTAGT 1 cut(s) 5027
SphI GCATGC 1 cut(s) 5590
SspI AATATT 4 cut(s) 496, 1593, 2971, 4002
StyI CCWWGG 6 cut(s) 1967, 3765, 3894, 4899, 5893, 6686
TaiI ACGT 7 cut(s) 3149, 3549, 3741, 4365, 4723, 5095, 6664
TaqII GACCGA 3 cut(s) 5628, 6245, 6296
TatI WGTACW 6 cut(s) 266, 917, 1312, 2175, 2391, 6367
TauI GCSGC 4 cut(s) 1673, 2222, 4562, 4898
TseFI GTSAC 7 cut(s) 1319, 2657, 2735, 2820, 3970, 4807, 5289
Tsp45I GTSAC 7 cut(s) 1319, 2657, 2735, 2820, 3970, 4807, 5289
TspGWI ACGGA 5 cut(s) 653, 1253, 1618, 2808, 5624
Van91I CCANNNNNTGG 2 cut(s) 4463, 5200
VneI GTGCAC 1 cut(s) 6765
VpaK11BI GGWCC 7 cut(s) 1794, 2089, 2537, 4163, 4460, 5611, 6496
VspI ATTAAT 2 cut(s) 1419, 5673
XagI CCTNNNNNAGG 2 cut(s) 1922, 2839
XapI RAATTY 9 cut(s) 259, 322, 486, 1021, 3092, 3220, 3915, 4018, 4472
XbaI TCTAGA 2 cut(s) 1297, 4054
XceI RCATGY 9 cut(s) 539, 1094, 1121, 1178, 1277, 3971, 3978, 4228, 5590
XcmI CCANNNNNNNNNTGG 4 cut(s) 999, 3402, 4087, 4844
XhoI CTCGAG 2 cut(s) 328, 1703
XmiI GTMKAC 1 cut(s) 6694
XmnI GAANNNNTTC 2 cut(s) 219, 3511
ZrmI AGTACT 3 cut(s) 268, 919, 2393
Zsp2I ATGCAT 3 cut(s) 1288, 2305, 5959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.