MD02G1214300.v1.1

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005282
Physical Location & Seq
Forward (+)
23803418 .. 23829088
25671 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1214300.v1.1.491

Sequence Viewer

Length: 7164 bp
ATGCTGAAGTTTACGCCGGCCACCGCCAGCCAGATTCGCTTCCTCCTCCAGAGCCTCAACGACGCCAACTCCGACTCCATTCTTCGAGAGCTCTCTCAGTTTATTGAATATGGAATTGAGGGAAGCATTTTGCTGTTACAGACCTGTCTGGATCATCTCACTAATTACAGAACAGAGTTAAAGAATGCAGCATTGGAGCGGGTTTTTGCCTCAATTTTCAAATCTCTCCTTGATAGACCTAATTTCAGTACGGTGTTTTGTCAGTCCCTAAGAAATACAGAGATTAGCGAAGGAATTCTTGAGAATGTCTCTAATGCATTACACCTGTCTGTATCTGAGAAAACTGGCATTGCTCTTGCGTTGTCAGATTCTGAAAACCTAGACACCAGGATATGTGGCAAGAACTTCTGTATGGCTCAGATCCAGAAGTTGTGTGAAAATGCTAATGTTATGAATTCATCTGAGCAAATTCATAACATTGTTATGTTCCTCCACCGGTCTGAGGGCCTTTCCAAGCATGTGGATTCCTTTATGCAGATGTTATCTTTACTGCAGCTGAAAGATGACAGTTTTTCTGTTTTAACTCCATTGCTTTCAGATGAGCTCCGTGATGCCAATTTTTTGAGCAATGTGGATTTGTTGCATGAAAACGGAGAAAATAATTTTGATGCTATTTTATCTGAAATGGAGAAGGAAATCAGCATGGGAGATATAATGAAGGAATTAGGTTATGGATGCACAGTTGATTCATCTCAGTGCAGCGAGATTTTATCTCTTTTCTTACCTCTCACTGAGATTACTATTTCAAAAATCCTTGGTATGATTGCCTCTACTCATGTTGGTCTTGAGGACAATCAGAATACATTTTCAACGTTCTGGTTGGCGCTTGGTAACAGCACTTTATCCGATCTGCCTATGTTGAACACCTGGAATATTGATGTCCTTGTAGATACAATCATGCAACTTGCTCCCGGCACTAACTGGATACAAGTTATTGAAAATTTGGATCATGAGGGATTCTACATTCCTAATCAGGAGGCTTTCTCTTTTTTCATGTCCGTGTATCAGCATGTGTGTCAGGATCCATTCCCCCTCCATGTTATCTGCAGGTCTGTTTGGAAGAATACCGAGGGTCAGTTATCCTTCCTTAAACATGCTATATCAGCACCACCTGAAGTGTTTACCTTTGCCCACTCTGTTAGGCAACTGGCCTATATTGATGCAGTGCATGGCCATAAGCTTCAACTTGGACATGCAAATCATGCATGGTTGAGTCTTGACCTTTTGGATGTACTGTGTCTACTAGCTGAGAGGGGTCATGCTCTTCCTGTTCGATCAATGCTTGAGCATCCTCTTAAACACTGTCCTGAAGTCCTGCTTCTTGGGATGGCCCATACAAATACTCTGTATAACCTGCTGCAATATGAAGTGTCTTATACCGTTTTCCCCATGATGGTAAAAAATGCAACGGGCAGTGGCATAATTAACCACCTCTGGCATATTAACACCTCACTGGTGCTGCGGGGATTTATGGATTCTCACAATTGTGATCCAGATAGCATAGCTAGAATATTGGATATCTGTCAAGAGCTAAAGATTCTATCATCTGTCCTAGAGATGATCCCTTCCTCTTTTAGTATCAGACTGGCAGCCCTTGCTTCCAGAAAAGAGCTTGTAGACCTTGAGAAGTGGTTGAGTAATAATTTGAATGCGCATAAGGATATTTTCTTTGAGGAGTGCATAAAGTTCTTGAAGGAGATTCAATTCAGTGGGTCACAGGATTTCTCCACCAGACCTTTCCAACATTCTGGTGCCGTGTCTAATATTTTTGTGGACACTGCTACTATATTCTTGAAGGTTCTTAAAGCTCATGTTGGCTTGATTGCCTCTAGCCAACTTACTGAGGAGTTGGAAAGGTTGTCTGTAACAATTATGGATTCTAATCCAAGGCTGCAGAATTGTGACCCTACGGAGTCAACTGATGGATATGCAGATGATATTGAAGCTGAAGCAAACTCTTACTTCCATCAAATGTTTTCTGGTCAGTTGACAATTGATTCAATGGTCCAAATGCTTGCCCGATTCAAGGAATCTTCTATAAAAAGGGAACAGTCAATTTTTGAGTGCATGATTGCAAATCTGTTTGAAGAATATAGATTTTTCCCCAAGTATCCTGAAAGACAGCTCAAAATTGCCGCAGTTCTGTTTGGCTCTGTCATCAAACACCAGCTTGTAACTCATCTGACTCTTGGGATCGCTCTGCGTGGTGTTTTAGATGCATTGCGCAAACCTGCGGATTCAAAAATGTTTGTGTTTGGAACTAAGGCCTTGGAGCAGTTTGTGGATCGTCTGATTGAGTGGCCTCAGTATTGCAATCATATTCTTCAAATATCCCATCTGCAGAGTACTCATTCAGAGCTTGTGGCTTTCATTGAACAGGCTCTTGCCAGGATTTCATTAGGCCATTTAGATTCAGATGGAAGTAACCATGCTTCCGCTGCTCATCACCATGGTCCCAGTCAAGCTACCCCAGGAAATGTAGAGTTAAATGGCTCTACCATCTTACATACCGGGCAACAACTTTCTTCACCACTCCAGCTTCAACAGAGACATGAAAGTTCTCTTGATGACCATCATAAAGCCTCAAATTCATCAAATGACATAAAGCCACCTTTATCTTCTGCAGTGCAACCTCCGTCACAAAGTGCACTCGGTGCTCCAGCAATGTTGTCTTCTGCTTCCCCTGGTTTTGTTCGTCCTTCTCGAGGAGTTACTTCTACCAGGTTTGGCTCTGCTTTGAATATTGAAACATTAGTTGCTGCTGCCGAGAAAAGAGATACTCCCATTGAGGCTCCAGCATCAGAGATTCAGGACAAGATATCATTTATAATTAATAATATTTCAGTTGCCAATGTTGAAGCTAAGTCAAAGGAGTTTGCTGAAATAATGAAAGAGCAGTACCATCCTTGGTTTGCGCAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCGAATTTTCATGATTTGTACCTGAAATTTCTGGACAAAGTTAATTCTAAGTGTTTGAATAAGGAGATTGTTCAAGCCACTTACGAGAACTGCAAGGTTCTTCTTGGATCTGAGCTTATAAAATCAAGCTCAGAAGAGCGTTCATTGTTAAAAAATTTAGGCAGCTGGCTTGGGAAACTTACAATTGGAAGGAATCAAGTCTTAAGGGCTCGGGAAATAGATCCCAAATCTTTGATTATAGAGGCATATGAAAAGGGGTTGATGATTGCAGTTATACCCTTTACTTCAAAGATTCTGGAGCCATGCCAAAGTAGCTTGGCATATCAGCCACCAAATCCTTGGACAATGGGTATTCTTGGATTACTTGCTGAGATATATTCAATGCCAAACTTGAAAATGAACCTGAAGTTTGATATTGAGGTTCTATTCAAGAATCTTGGTGTGGATTTGAAGGAGATATCCCCAAGTTCTCTTCTCAAGGACCGGAACAGAGAACTTGAAGGGAATCCTGATTTCTCTAACAAAGATATTGGATCATCCCAACCACAGATGGTTGCTGATGTGAAATCCGGAATGTTATCTCCGCTAAATCAGGTTGAACTACCCGTTGAGGTTGCTCCATCTAATTCTGGAAGCCATACACATCTACTATCTCAGTATGCGCCCCCTGTTCATATTTCAACTGGCACCTTAATTGAGGATGAAAAGCTGTTGCCTGATCAGATTCCTGCTCAAGGACTGTTACAAGCTACTCCATCCCAGTCACCATTCTCTGTTCCCACACCAATATCTAATATTGCAACTCACGTGGTTATCAACCCGAAACTCAGTGCTTTGGGATTGCACTTGCATTTTCAGAGGGTGGTTCCGATTGCAATGGACAGAGCTATCAAAGAGATAGTATCTGGTATCGTTCAGCGCAGTGTTTCTATAGCTACTCAGACAACTAAAGAACTTGTCTTAAAGGATTATGCCATGGAATCTGATGAGACAAGAATATTATATGCAGCTCACTTGATGGTTGCAAGTTTGGCAGGAAGTCTAGCTCATGTAACTTGCAAGGAACCCTTGCGTACTTCTATATCAACTCAGCTGCGAAATTCCCTTCAGGGATCAAACATTCCAAATGATCATCTGGAACATGCTGTACAACTTGTTACTAATGACAATCTGGATCTTGGCTGTGCAATCATTGAACAGGCTGCTACTGATAAGGCATTACAAACAATTGATGGAGAAATAGCTCAACAACTGTCATTAAGAAGGAAGAGGGAAGGTGCGGGTACAACATTTTTTGATGCTAACATGTATACACAAAGTGGCAGTGTGCCTGAGTCCCTTCGCCCTAAACCTGGTCATTTGTCCATTTCTCAACAACGAGTTTATGAGGACTTCGTGCGGCTTCCCTGGCAAAACCAGTCTAGCCAGAATTCACATGTTCTTCCTGCTAGTACTGCAGCTCCATCTGCAAGTGCTGGTCTAAATAGCATATTTGGTTCAGCCTCGGTTCAACTTAACACAGGCTACTCAGCAGGTCCTGGAAGCAAGTTTGATGTTGTTTCTCGTCCACTGGATGAGGCAGTTGAACCCAATCCAGCTCTGCATTCAAGTGCATCATCTATTTGTGTTGGAGCGGGTGATGGTGTTTCCCAGCATAGTTCTGAGAATGATTCTGTCATTGGTTCATTTCTTTCTGCTGCTTCTGCCCCAGAGCTGCAGTCAGTAGAGTCGTCTGATGCTATAAAGGAATCTGGAATTTCTTCACAGCCGCTACCTTCATCTACTGCAACTGAACTTCTGGCATGCAACATCTCAGAACCTTCTCTTAATACGAGGGATGCATTGGATAAATACCAGATTGTTTCACAAAAGCTTGAAGCTTTGGTGACCAACGATGCTAAAGAGTCAGAAATTCAGGGAGTAATTGGTGAAGTTCCTGAGATTATACTCAGATGTGTTAGTCGAGATGAGGCTGCCTTGGCTGTGGCTCAAAAGGTTTTCAAGGGTTTATACGAGAATGCATCGAACCATACTCACGTCAGTGCTCATATTGCAATGCTGACAGCTATTCGTGATGTTTGCAAACTTGTTGTTAAGGAGCTCACTAGTTGGGTTATTTATTCTGATGAAGAGCGGAAGTTTAACAAAGATATTACTGTTGGCCTTATTCACAGTGAATTACTAAATCTTGCAGAGTACAATGTTCAAATGGAAAAACTCATTGATGGGGGCAGAAATAAGCCTGCAACTGAATTTTCAATTTCCCTTCTCCAATCTTTGGTGATTGAAGAATCTAAAGTTATATCAGAACTTCAAAATCTTGTTGACACATTGGCAAAGCTCGCTGCAAAGCCTGGATTTCCTGAGTCGCTACAGCAGCTGGTTGAAATGGTAAAGTATCCTGCTTCTACTGTGGGTGCTCCGTCTGCTATTAATGTAGGAAAAGAGGACAAGGGAAGAATATCTAAGGATAAAAAGGCTTCTGTTCACTCTCCGGTGAGCAGGGAAGACTTTAGTAATGTGGAGTCTGTCGAACCAGATCCTCCTGGATTCCGTGAGCAGGTGTCAATGCTGTTTGCAGAGTGGTACCGGATTTGTGAACTTCCTGGTGCAAATGATGCAGCTTATACCCATTTTATCTTGCAGCTACATCAAAATGGTCTGCTGAAAGGGGATGATATGACAGACCGGTTTTTCCGTGTCCTCACAGAGCTATCTGTTGCACATTGCCTGTCTTCTGAGATGACAATTCCCGGGACACTGCAAACACCTCAACAAGTGCCGAATTTCTCCTTCCTTGCTATTGATATCTATGCTAAACTTGTTTTCTTAATTTTGAAGGGATCAAATAAACTATTTCTCTTGTCCAAGATTTTAGCAGTCATAGTGAGATTCATTCAAAAGGATGCAGAGGAGAAAAAAGGATCTTTCAACCCAAGACCGTATTTTAGATTGATTGTTAACTGGCTTCTAGACCTTGGTTCTTTGGATCCTGTGATTGATGGTGCTAACTTTCAGATATTGACAGCATTTGCAAATGCATTTCATGCTTTGCAGCCCCTTAAGGTTCCTACATTCAGCTTTGCATGGCTTGAGTTGGTGAGTCATAGGAGTTTCATGCCAAAAATGCTTGCTGGAAATGGCCAGAAGGGTTGGCCCTATATCCAACGTTTGCTGGTACATTTGTTTCAATTCATGGAGCCATTTTTGAGGAATGCTGAACTTGGAGTCCCGGTGCATTTCCTGTATAAAGGCACACTTAGGGTGCTGCTAGTGCTACTCCATGACTTTCCAGAGTTCCTTTGTGATTATCATTTTACGTTCTGTGATGTAATTCCTCCAAGCTGCATTCAAATGCGAAATATCATCCTCAGTGCATTTCCCCGTAATATGAGGCTACCAGATCCATCTACTCCCAACTTAAAGATTGATTTGCTCGCTGAAATCAGTCAGTCTCCACGTATTCTCTCTGAGGTTGATGCAGCTCTAAAAGCGAAGCAGATGAAAGCTGATGTGGATGAGTACCTCAAGACAAGGCAACAGGGTTCTTCATTTCTGACTGAACTGAAGCAAAAGTTGCTCCTTTTACCAAGTGAAGCTGCCTCAGCTGGTACCCGTTACAATGTACCCCTGATCAACTCCCTTGTGCTTTATGTTGGGATGCAGGCTATCCAGCAGCTTCAGGCTAGAACGCCTCGAGCGCAATCTACCCAAACTGTTCCATTGGCTGTCTATTTGGTGGGTGCTGCTTTGGATATTTTTCAGACTTTGATGGTGGACCTAGATACTGAAGGGCGCTACCTTTTCCTAAATGCGATTGCAAACCAACTGCGTTATCCAAACACCCATACCCATTACTTTTCCTTCATTGTTCTGTACTTGTTTGCCGATTCAAACCAGCATGAAATTATCCAGGAGCAAATCACAAGGGTATTGTTGGAACGTCTGATTGTTAACCGACCTCATCCATGGGGTCTTCTGATTACCTTCATTGAGCTTATCAAGAATCCGAGATACCAGTTCTGGAACCGAGCATTCATCAGATGTGCACCGGAGATTGAAAAACTTTTCGAATCTGTCTCAAGGTCTTGTGGCGGTCCAAAACCTGTGGACGAAAGTATGGTCTCAGGTTGGGTATCGGAGAGTGCCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2388

Amino Acids

265.11

Weight (kDa)

5.69

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 476 - 622 4e-37 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 657 - 816 1.4e-67 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 949 - 1168 1e-89 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1287 - 1426 6.9e-50 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
ARM_Cnot1 PF25097 1628 - 1802 1.1e-53 Cnot1 ARM repeat
NOT1_connector PF23590 1651 - 1801 1.4e-16 CCR4-NOT transcription complex subunit 1, NOT1_connector
Not1 PF04054 2002 - 2363 6.8e-142 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2888, 3128
AarI CACCTGC 1 cut(s) 5560
Acc16I TGCGCA 3 cut(s) 1713, 2285, 2976
Acc36I ACCTGC 5 cut(s) 1098, 1422, 2299, 4532, 5560
Acc65I GGTACC 2 cut(s) 5595, 6620
AccB1I GGYRCC 4 cut(s) 1811, 3695, 5595, 6620
AccB7I CCANNNNNTGG 1 cut(s) 5287
AccBSI CCGCTC 3 cut(s) 199, 4643, 5143
AccI GTMKAC 3 cut(s) 1300, 1677, 4319
AccIII TCCGGA 1 cut(s) 3578
AclI AACGTT 2 cut(s) 872, 6178
AcoI YGGCCR 3 cut(s) 18, 1231, 6151
AcuI CTGAAG 9 cut(s) 26, 1194, 1389, 2028, 3434, 4100, 6596, 6674, 6819
AcvI CACGTG 1 cut(s) 3817
AcyI GRCGYC 1 cut(s) 63
AdeI CACNNNGTG 3 cut(s) 2705, 2714, 4328
AfiI CCNNNNNNNGG 8 cut(s) 502, 1453, 2086, 2765, 3743, 4361, 5287, 5569
AflII CTTAAG 2 cut(s) 3211, 6071
AflIII ACRYGT 2 cut(s) 4314, 4444
AgeI ACCGGT 2 cut(s) 495, 5697
AhlI ACTAGT 1 cut(s) 5114
AjiI CACGTC 1 cut(s) 5047
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AleI CACNNNNGTG 2 cut(s) 1545, 5049
AloI GAACNNNNNNTCC 2 cut(s) 6222, 6254
Alw21I GWGCWC 8 cut(s) 93, 606, 2710, 2719, 5056, 5112, 5431, 7060
Alw26I GTCTC 6 cut(s) 313, 2602, 3992, 6468, 7093, 7138
Alw44I GTGCAC 2 cut(s) 2706, 7056
AlwNI CAGNNNCTG 3 cut(s) 371, 4547, 5389
Ama87I CYCGRG 4 cut(s) 2763, 3219, 5762, 6705
Aor13HI TCCGGA 1 cut(s) 3578
ApaLI GTGCAC 2 cut(s) 2706, 7056
ArsI GACNNNNNNTTYG 2 cut(s) 7116, 7148
AseI ATTAAT 2 cut(s) 2892, 5442
AsiGI ACCGGT 2 cut(s) 495, 5697
Asp700I GAANNNNTTC 2 cut(s) 294, 4768
Asp718I GGTACC 2 cut(s) 5595, 6620
AspLEI GCGC 8 cut(s) 886, 1714, 2286, 2977, 3673, 3930, 6712, 6807
AspS9I GGNCC 9 cut(s) 505, 1390, 2065, 2513, 3490, 4544, 6165, 6787, 7106
AsuC2I CCSGG 5 cut(s) 972, 2572, 5763, 5764, 6242
AsuHPI GGTGA 9 cut(s) 2498, 2580, 3765, 4658, 4906, 4949, 5302, 5518, 6121
AsuII TTCGAA 1 cut(s) 7080
AvaI CYCGRG 4 cut(s) 2763, 3219, 5762, 6705
AvaII GGWCC 6 cut(s) 2065, 2513, 3490, 4544, 6787, 7106
BaeGI GKGCMC 3 cut(s) 2710, 7060, 7159
BaeI ACNNNNGTAYC 2 cut(s) 6179, 6212
BalI TGGCCA 2 cut(s) 1233, 6153
BamHI GGATCC 2 cut(s) 1081, 5998
BanI GGYRCC 4 cut(s) 1811, 3695, 5595, 6620
BanII GRGCYC 4 cut(s) 93, 606, 3220, 5112
BarI GAAGNNNNNNTAC 2 cut(s) 2476, 2508
BbrPI CACGTG 1 cut(s) 3817
BbsI GAAGAC 4 cut(s) 2724, 5523, 5736, 6977
Bbv12I GWGCWC 8 cut(s) 93, 606, 2710, 2719, 5056, 5112, 5431, 7060
BbvCI CCTCAGC 1 cut(s) 6613
BceAI ACGGC 1 cut(s) 1799
BciVI GTATCC 3 cut(s) 978, 2181, 5418
BclI TGATCA 3 cut(s) 3727, 4138, 6642
BcnI CCSGG 5 cut(s) 972, 2572, 5763, 5764, 6242
BcoDI GTCTC 6 cut(s) 313, 2602, 3992, 6468, 7093, 7138
BcuI ACTAGT 1 cut(s) 5114
BfmI CTRYAG 9 cut(s) 551, 1105, 1952, 2399, 2682, 3939, 4464, 4724, 5381
BfoI RGCGCY 2 cut(s) 887, 6808
BfrI CTTAAG 2 cut(s) 3211, 6071
BfuAI ACCTGC 5 cut(s) 1098, 1422, 2299, 4532, 5560
BfuI GTATCC 3 cut(s) 978, 2181, 5418
BmcAI AGTACT 2 cut(s) 2407, 4462
Bme18I GGWCC 6 cut(s) 2065, 2513, 3490, 4544, 6787, 7106
BmeT110I CYCGRG 4 cut(s) 2763, 3219, 5762, 6705
BmgBI CACGTC 1 cut(s) 5047
BmgT120I GGNCC 9 cut(s) 505, 1390, 2065, 2513, 3490, 4544, 6165, 6787, 7106
BmrI ACTGGG 2 cut(s) 2511, 3763
BmuI ACTGGG 2 cut(s) 2511, 3763
BpiI GAAGAC 4 cut(s) 2724, 5523, 5736, 6977
BplI GAGNNNNNCTC 4 cut(s) 293, 325, 1028, 1060
BpmI CTGGAG 5 cut(s) 32, 2579, 2703, 2838, 3326
Bpu10I CCTNAGC 1 cut(s) 6613
Bpu14I TTCGAA 1 cut(s) 7080
BpuEI CTTGAG 9 cut(s) 320, 866, 1364, 1703, 3470, 3726, 6122, 6521, 7075
BpuMI CCSGG 5 cut(s) 972, 2572, 5763, 5764, 6242
BsaAI YACGTR 2 cut(s) 3817, 6470
BsaBI GATNNNNATC 2 cut(s) 1941, 2631
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 7138
BsaWI WCCGGW 7 cut(s) 495, 3492, 3578, 5503, 5598, 5697, 7060
BsaXI ACNNNNNCTCC 8 cut(s) 53, 59, 83, 89, 4453, 4483, 6228, 6258
Bsc4I CCNNNNNNNGG 8 cut(s) 502, 1453, 2086, 2765, 3743, 4361, 5287, 5569
Bse118I RCCGGY 3 cut(s) 16, 495, 5697
Bse3DI GCAATG 8 cut(s) 348, 587, 634, 2279, 2730, 3891, 5070, 5734
Bse8I GATNNNNATC 2 cut(s) 1941, 2631
BseAI TCCGGA 1 cut(s) 3578
BseJI GATNNNNATC 2 cut(s) 1941, 2631
BseLI CCNNNNNNNGG 8 cut(s) 502, 1453, 2086, 2765, 3743, 4361, 5287, 5569
BseMI GCAATG 8 cut(s) 348, 587, 634, 2279, 2730, 3891, 5070, 5734
BseRI GAGGAG 5 cut(s) 35, 1748, 1919, 2781, 5936
BseSI GKGCMC 3 cut(s) 2710, 7060, 7159
BseYI CCCAGC 1 cut(s) 4659
BsgI GTGCAG 1 cut(s) 778
BshNI GGYRCC 4 cut(s) 1811, 3695, 5595, 6620
BshTI ACCGGT 2 cut(s) 495, 5697
BsiHKAI GWGCWC 8 cut(s) 93, 606, 2710, 2719, 5056, 5112, 5431, 7060
BsiHKCI CYCGRG 4 cut(s) 2763, 3219, 5762, 6705
BslFI GGGAC 5 cut(s) 250, 2499, 4330, 5779, 6224
BslI CCNNNNNNNGG 8 cut(s) 502, 1453, 2086, 2765, 3743, 4361, 5287, 5569
BsmAI GTCTC 6 cut(s) 313, 2602, 3992, 6468, 7093, 7138
BsmFI GGGAC 5 cut(s) 250, 2499, 4330, 5779, 6224
BsmI GAATGC 7 cut(s) 190, 1714, 4612, 5032, 6229, 6357, 7043
Bso31I GGTCTC 1 cut(s) 7138
BsoBI CYCGRG 4 cut(s) 2763, 3219, 5762, 6705
Bsp119I TTCGAA 1 cut(s) 7080
Bsp13I TCCGGA 1 cut(s) 3578
Bsp1407I TGTACA 1 cut(s) 4156
Bsp19I CCATGG 3 cut(s) 2509, 3984, 6977
BspEI TCCGGA 1 cut(s) 3578
BspHI TCATGA 2 cut(s) 1009, 3019
BspMAI CTGCAG 7 cut(s) 555, 1109, 1956, 2403, 2686, 4468, 4728
BspMI ACCTGC 5 cut(s) 1098, 1422, 2299, 4532, 5560
BspQI GCTCTTC 3 cut(s) 1329, 3138, 5133
BspT104I TTCGAA 1 cut(s) 7080
BspT107I GGYRCC 4 cut(s) 1811, 3695, 5595, 6620
BspTI CTTAAG 2 cut(s) 3211, 6071
BspTNI GGTCTC 1 cut(s) 7138
BsrBI CCGCTC 3 cut(s) 199, 4643, 5143
BsrDI GCAATG 8 cut(s) 348, 587, 634, 2279, 2730, 3891, 5070, 5734
BsrFI RCCGGY 3 cut(s) 16, 495, 5697
BsrGI TGTACA 1 cut(s) 4156
BssAI RCCGGY 3 cut(s) 16, 495, 5697
BssNAI GTATAC 1 cut(s) 4320
BssNI GRCGYC 1 cut(s) 63
Bst1107I GTATAC 1 cut(s) 4320
Bst6I CTCTTC 5 cut(s) 1329, 3138, 3486, 4271, 5133
BstACI GRCGYC 1 cut(s) 63
BstAFI CTTAAG 2 cut(s) 3211, 6071
BstAPI GCANNNNNTGC 6 cut(s) 1262, 1655, 2714, 5627, 6056, 6586
BstAUI TGTACA 1 cut(s) 4156
BstBAI YACGTR 2 cut(s) 3817, 6470
BstBI TTCGAA 1 cut(s) 7080
BstDSI CCRYGG 3 cut(s) 2509, 3984, 6977
BstEII GGTNACC 1 cut(s) 4894
BstENI CCTNNNNNAGG 2 cut(s) 2763, 3741
BstH2I RGCGCY 2 cut(s) 887, 6808
BstHHI GCGC 8 cut(s) 886, 1714, 2286, 2977, 3673, 3930, 6712, 6807
BstMAI GTCTC 6 cut(s) 313, 2602, 3992, 6468, 7093, 7138
BstNSI RCATGY 8 cut(s) 521, 1073, 1157, 1256, 4154, 4318, 4448, 4815
BstPI GGTNACC 1 cut(s) 4894
BstSFI CTRYAG 9 cut(s) 551, 1105, 1952, 2399, 2682, 3939, 4464, 4724, 5381
BstSLI GKGCMC 3 cut(s) 2710, 7060, 7159
BstV2I GAAGAC 4 cut(s) 2724, 5523, 5736, 6977
BstX2I RGATCY 9 cut(s) 420, 1081, 3116, 3229, 4183, 5548, 5933, 5998, 6412
BstXI CCANNNNNNTGG 3 cut(s) 520, 1808, 3348
BstYI RGATCY 9 cut(s) 420, 1081, 3116, 3229, 4183, 5548, 5933, 5998, 6412
BstZ17I GTATAC 1 cut(s) 4320
BsuI GTATCC 3 cut(s) 978, 2181, 5418
BtgI CCRYGG 3 cut(s) 2509, 3984, 6977
BtrI CACGTC 1 cut(s) 5047
BtsI GCAGTG 7 cut(s) 1230, 1480, 1836, 2691, 3937, 4339, 5768
BveI ACCTGC 5 cut(s) 1098, 1422, 2299, 4532, 5560
CaiI CAGNNNCTG 3 cut(s) 371, 4547, 5389
CciI TCATGA 2 cut(s) 1009, 3019
CfoI GCGC 8 cut(s) 886, 1714, 2286, 2977, 3673, 3930, 6712, 6807
Cfr10I RCCGGY 3 cut(s) 16, 495, 5697
Cfr13I GGNCC 9 cut(s) 505, 1390, 2065, 2513, 3490, 4544, 6165, 6787, 7106
Cfr9I CCCGGG 1 cut(s) 5762
CseI GACGC 1 cut(s) 71
CsiI ACCWGGT 2 cut(s) 2780, 4360
CspAI ACCGGT 2 cut(s) 495, 5697
CspCI CAANNNNNGTGG 4 cut(s) 3798, 3833, 7098, 7133
DraIII CACNNNGTG 3 cut(s) 2705, 2714, 4328
EaeI YGGCCR 3 cut(s) 18, 1231, 6151
Eam1104I CTCTTC 5 cut(s) 1329, 3138, 3486, 4271, 5133
EarI CTCTTC 5 cut(s) 1329, 3138, 3486, 4271, 5133
Ecl136II GAGCTC 3 cut(s) 91, 604, 5110
Eco147I AGGCCT 1 cut(s) 2327
Eco24I GRGCYC 4 cut(s) 93, 606, 3220, 5112
Eco31I GGTCTC 1 cut(s) 7138
Eco32I GATATC 4 cut(s) 1579, 2880, 3468, 5818
Eco47I GGWCC 6 cut(s) 2065, 2513, 3490, 4544, 6787, 7106
Eco53kI GAGCTC 3 cut(s) 91, 604, 5110
Eco57I CTGAAG 9 cut(s) 26, 1194, 1389, 2028, 3434, 4100, 6596, 6674, 6819
Eco72I CACGTG 1 cut(s) 3817
Eco88I CYCGRG 4 cut(s) 2763, 3219, 5762, 6705
Eco91I GGTNACC 1 cut(s) 4894
EcoICRI GAGCTC 3 cut(s) 91, 604, 5110
EcoNI CCTNNNNNAGG 2 cut(s) 2763, 3741
EcoO109I RGGNCCY 2 cut(s) 505, 4544
EcoO65I GGTNACC 1 cut(s) 4894
EcoRI GAATTC 3 cut(s) 294, 454, 4438
EcoRV GATATC 4 cut(s) 1579, 2880, 3468, 5818
EcoT22I ATGCAT 6 cut(s) 319, 1267, 2281, 4852, 5032, 6052
EcoT38I GRGCYC 4 cut(s) 93, 606, 3220, 5112
FalI AAGNNNNNCTT 8 cut(s) 282, 314, 1362, 1394, 4061, 4093, 6252, 6284
FaqI GGGAC 5 cut(s) 250, 2499, 4330, 5779, 6224
FauI CCCGC 4 cut(s) 192, 1515, 4282, 4636
FauNDI CATATG 1 cut(s) 3256
FbaI TGATCA 3 cut(s) 3727, 4138, 6642
FblI GTMKAC 3 cut(s) 1300, 1677, 4319
FriOI GRGCYC 4 cut(s) 93, 606, 3220, 5112
FspAI RTGCGCAY 1 cut(s) 1713
FspI TGCGCA 3 cut(s) 1713, 2285, 2976
GlaI GCGC 8 cut(s) 885, 1713, 2285, 2976, 3672, 3929, 6711, 6806
GsaI CCCAGC 1 cut(s) 4663
GsuI CTGGAG 5 cut(s) 32, 2579, 2703, 2838, 3326
HaeII RGCGCY 2 cut(s) 887, 6808
HgaI GACGC 1 cut(s) 71
HhaI GCGC 8 cut(s) 886, 1714, 2286, 2977, 3673, 3930, 6712, 6807
Hin1I GRCGYC 1 cut(s) 63
Hin6I GCGC 8 cut(s) 884, 1712, 2284, 2975, 3671, 3928, 6710, 6805
HinP1I GCGC 8 cut(s) 884, 1712, 2284, 2975, 3671, 3928, 6710, 6805
HincII GTYRAC 5 cut(s) 1977, 2049, 5335, 5971, 6964
HindII GTYRAC 5 cut(s) 1977, 2049, 5335, 5971, 6964
HindIII AAGCTT 3 cut(s) 1238, 4880, 4887
HpaI GTTAAC 2 cut(s) 5971, 6964
HphI GGTGA 9 cut(s) 2498, 2580, 3765, 4658, 4906, 4949, 5302, 5518, 6121
Hpy99I CGWCG 1 cut(s) 65
HpyCH4IV ACGT 7 cut(s) 872, 3816, 5046, 6178, 6329, 6469, 6952
HpySE526I ACGT 7 cut(s) 872, 3816, 5046, 6178, 6329, 6469, 6952
Hsp92I GRCGYC 1 cut(s) 63
HspAI GCGC 8 cut(s) 884, 1712, 2284, 2975, 3671, 3928, 6710, 6805
Kpn2I TCCGGA 1 cut(s) 3578
KpnI GGTACC 2 cut(s) 5599, 6624
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Ksp22I TGATCA 3 cut(s) 3727, 4138, 6642
KspAI GTTAAC 2 cut(s) 5971, 6964
LguI GCTCTTC 3 cut(s) 1329, 3138, 5133
MabI ACCWGGT 2 cut(s) 2780, 4360
MaeII ACGT 7 cut(s) 872, 3816, 5046, 6178, 6329, 6469, 6952
MbiI CCGCTC 3 cut(s) 199, 4643, 5143
MfeI CAATTG 4 cut(s) 1543, 2052, 3192, 4236
MflI RGATCY 9 cut(s) 420, 1081, 3116, 3229, 4183, 5548, 5933, 5998, 6412
MlsI TGGCCA 2 cut(s) 1233, 6153
MluNI TGGCCA 2 cut(s) 1233, 6153
MmeI TCCRAC 6 cut(s) 96, 1825, 1890, 4618, 6199, 6927
Mox20I TGGCCA 2 cut(s) 1233, 6153
Mph1103I ATGCAT 6 cut(s) 319, 1267, 2281, 4852, 5032, 6052
MroI TCCGGA 1 cut(s) 3578
MroNI GCCGGC 1 cut(s) 16
MroXI GAANNNNTTC 2 cut(s) 294, 4768
MscI TGGCCA 2 cut(s) 1233, 6153
Msp20I TGGCCA 2 cut(s) 1233, 6153
MspA1I CMGCKG 6 cut(s) 556, 2498, 3174, 4102, 5389, 6617
MspCI CTTAAG 2 cut(s) 3211, 6071
MunI CAATTG 4 cut(s) 1543, 2052, 3192, 4236
Mva1269I GAATGC 7 cut(s) 190, 1714, 4612, 5032, 6229, 6357, 7043
NaeI GCCGGC 1 cut(s) 18
NciI CCSGG 5 cut(s) 972, 2572, 5763, 5764, 6242
NcoI CCATGG 3 cut(s) 2509, 3984, 6977
NdeI CATATG 1 cut(s) 3256
NgoMIV GCCGGC 1 cut(s) 16
NmeAIII GCCGAG 1 cut(s) 2851
NmuCI GTSAC 5 cut(s) 1773, 1961, 2697, 3771, 4894
NsbI TGCGCA 3 cut(s) 1713, 2285, 2976
NsiI ATGCAT 6 cut(s) 319, 1267, 2281, 4852, 5032, 6052
NspI RCATGY 8 cut(s) 521, 1073, 1157, 1256, 4154, 4318, 4448, 4815
NspV TTCGAA 1 cut(s) 7080
OliI CACNNNNGTG 2 cut(s) 1545, 5049
PaeI GCATGC 1 cut(s) 4815
PaeR7I CTCGAG 2 cut(s) 2763, 6705
PagI TCATGA 2 cut(s) 1009, 3019
PaqCI CACCTGC 1 cut(s) 5560
PceI AGGCCT 1 cut(s) 2327
PciI ACATGT 2 cut(s) 4314, 4444
PciSI GCTCTTC 3 cut(s) 1329, 3138, 5133
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 2761
PctI GAATGC 7 cut(s) 190, 1714, 4612, 5032, 6229, 6357, 7043
PdiI GCCGGC 1 cut(s) 18
PdmI GAANNNNTTC 2 cut(s) 294, 4768
PflMI CCANNNNNTGG 1 cut(s) 5287
PfoI TCCNGGA 3 cut(s) 4546, 5554, 6921
PinAI ACCGGT 2 cut(s) 495, 5697
PmaCI CACGTG 1 cut(s) 3817
PmlI CACGTG 1 cut(s) 3817
Ppu21I YACGTR 2 cut(s) 3817, 6470
PpuMI RGGWCCY 1 cut(s) 4544
PscI ACATGT 2 cut(s) 4314, 4444
PshBI ATTAAT 2 cut(s) 2892, 5442
PsiI TTATAA 2 cut(s) 2888, 3128
Psp124BI GAGCTC 3 cut(s) 93, 606, 5112
Psp1406I AACGTT 2 cut(s) 872, 6178
Psp5II RGGWCCY 1 cut(s) 4544
PspCI CACGTG 1 cut(s) 3817
PspEI GGTNACC 1 cut(s) 4894
PspFI CCCAGC 1 cut(s) 4659
PspPI GGNCC 9 cut(s) 505, 1390, 2065, 2513, 3490, 4544, 6165, 6787, 7106
PspPPI RGGWCCY 1 cut(s) 4544
PspXI VCTCGAGB 1 cut(s) 6705
PsrI GAACNNNNNNTAC 2 cut(s) 4140, 4172
PstI CTGCAG 7 cut(s) 555, 1109, 1956, 2403, 2686, 4468, 4728
PstNI CAGNNNCTG 3 cut(s) 371, 4547, 5389
PsuI RGATCY 9 cut(s) 420, 1081, 3116, 3229, 4183, 5548, 5933, 5998, 6412
PvuII CAGCTG 5 cut(s) 556, 3174, 4102, 5389, 6617
SacI GAGCTC 3 cut(s) 93, 606, 5112
SapI GCTCTTC 3 cut(s) 1329, 3138, 5133
Sau96I GGNCC 9 cut(s) 505, 1390, 2065, 2513, 3490, 4544, 6165, 6787, 7106
ScaI AGTACT 2 cut(s) 2407, 4462
SexAI ACCWGGT 2 cut(s) 2780, 4360
SfcI CTRYAG 9 cut(s) 551, 1105, 1952, 2399, 2682, 3939, 4464, 4724, 5381
Sfr274I CTCGAG 2 cut(s) 2763, 6705
SfuI TTCGAA 1 cut(s) 7080
SinI GGWCC 6 cut(s) 2065, 2513, 3490, 4544, 6787, 7106
SlaI CTCGAG 2 cut(s) 2763, 6705
SmaI CCCGGG 1 cut(s) 5764
SpeI ACTAGT 1 cut(s) 5114
SphI GCATGC 1 cut(s) 4815
SseBI AGGCCT 1 cut(s) 2327
SspI AATATT 7 cut(s) 934, 1572, 1825, 2803, 2899, 3805, 4008
SstI GAGCTC 3 cut(s) 93, 606, 5112
StuI AGGCCT 1 cut(s) 2327
TaiI ACGT 7 cut(s) 875, 3819, 5049, 6181, 6332, 6472, 6955
TaqI TCGA 8 cut(s) 85, 1333, 2764, 4972, 5033, 5541, 6706, 7080
TatI WGTACW 6 cut(s) 1291, 2405, 4156, 4460, 5205, 6885
TauI GCSGC 3 cut(s) 2198, 4411, 4780
TseFI GTSAC 5 cut(s) 1773, 1961, 2697, 3771, 4894
Tsp45I GTSAC 5 cut(s) 1773, 1961, 2697, 3771, 4894
TspGWI ACGGA 8 cut(s) 596, 666, 1048, 1985, 2685, 5421, 5552, 5696
TspMI CCCGGG 1 cut(s) 5762
Van91I CCANNNNNTGG 1 cut(s) 5287
Vha464I CTTAAG 2 cut(s) 3211, 6071
VneI GTGCAC 2 cut(s) 2706, 7056
VpaK11BI GGWCC 6 cut(s) 2065, 2513, 3490, 4544, 6787, 7106
VspI ATTAAT 2 cut(s) 2892, 5442
XagI CCTNNNNNAGG 2 cut(s) 2763, 3741
XbaI TCTAGA 1 cut(s) 5980
XceI RCATGY 8 cut(s) 521, 1073, 1157, 1256, 4154, 4318, 4448, 4815
XcmI CCANNNNNNNNNTGG 1 cut(s) 3345
XhoI CTCGAG 2 cut(s) 2763, 6705
XmaI CCCGGG 1 cut(s) 5762
XmiI GTMKAC 3 cut(s) 1300, 1677, 4319
XmnI GAANNNNTTC 2 cut(s) 294, 4768
ZrmI AGTACT 2 cut(s) 2407, 4462
Zsp2I ATGCAT 6 cut(s) 319, 1267, 2281, 4852, 5032, 6052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.