RLG00000028628

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005282
Physical Location & Seq
Reverse (-)
25332323 .. 25349180
16858 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028628

Sequence Viewer

Length: 6603 bp
ATGTTTTGTGAATCACTGAGAAATACAGAGGTCAATGAAGGAATTGTGGAGAATTTCTCAAGTGTATTGCAACTGTCTGTACCTGAGAAAATTGGCATTGGACTTGCTTTGTCAGATTCAGAAAACCTAGACACCAGAATATGTGGCAAAAACTTCTGTATGGCTCAGATTGAGAAATTGTGTGAAACTCCTGTTGACAGGATCTCTTCTGAGAAAATTCAGAATATTGTTATGTTCCTTCAGCGGTCTGAGGGTCTTTCCAAGCATGTAGATTCCTTTATGCAGATATTATCTTTACTGCAGCTGAAAGATGATGAAAACTCTTTTGTTTTAAGTCCATTGCTTTCAGATGAGCTTCGTGATGCCAATTTTTTGAGGAACATGGGCTTGTTCCGTGAAAGCAGAGAAAATGATTTTGATGCTATTTTAGCAGAAATGGAGAAGGAGATGAGTATGGGAGATATTATGAAGGAATTAGGTTATGGATGCACAGTTGATTCATCTCAGTGCAAAGAGATTTTATCCCTTTTCTTACCCCTCAATGAGATTACTATCTCCAAAATACTTGGCACGATTGCCTGCACCCATGCTGGTCTTGAGGATAATCAGAATACATTTTCAACCTTCCGGTTGGCGCTTGGTTATAGTACTTCATCTGATTTGCCTATGCTAAACACCTGGAACATTGATGTCCTTGTGGATACGATCAATCAACTTGCTCCAGACACCAACTGGATAAGTGTTATTGAAAATTTGGATCACGAGGGGTTCTACATTCCTAATCAGGAGGCATTCTCTTTTTTCATGTCTGTATATCAGCATGTGTGTCAGGAGCCATTCCCCCTACATGCCATCTGTGGGTCTGTTTGGAAGAATACCGAGGGACAGCTATCTTTTCTCAAACATGCTGTTTCATCACCACCTGAAGTATTTAATTTTGCTCACTCTGATAGGCAGCTGCCCTATACGGATACGGTGAATGGCCACAAGCTACAACTTGGACATGCAAATCATGCATGGTTGTGTCTAGACCTTTTGGATGTACTTTGTCACCTAGCAGAGAGGGGTCATGCTCTTTCTGTTCGATCGATACTTGAGTATCCTCTTCAACACTGTCCTGAAGTCCTGCTTCTTGGGATGGCACACATTAATACTCCATACAACCTGCTGCAATATGAAGTGTCTTTCATTGTTTTCCCCATGATAGTAAAAAATATCATGGGTAATGGCATGATTGTTCACCTCTGGCATACTAACCCCTCTTTGGTGCTGCGGGGATTTTTGGATGCCTACAATAGCGATCCAGACAGCATGACCAGAATATTGGATATCTGTCAAGAGCTTAAGATTTTGTCGTCCGTCATGGAGATGATTCCTTCTCCCTTTAGTATCAGACTGGCGGCCCTTGCTTTCAGAAAAGAGCTTGTAGAACTCGAGAAGTGGTTGAGTAGTAATTTGAATACATATAAAGATACTTTCTTTGAGGAGTGCCTCAAGTTCCTGAAGGAGATTCAAGCTGGAGGGACCCAAGATTTCTCAACCAGACCTTTCCAACATTCTGGTGCTGTCTCAACTCTCTATGGGGACACTACTGCTACATTCTCGAAGGTTCTTAAAGCTCATGTTGCCCAGATTACTTCTAGCCACCTTACTGAGGAGATGGAAAAACTCTCTCTATCAATTATGGATTCAAATCCAAGGCTGCAGAATGGTGGGCCTACAGAGTCATCAACAGATGGATATGCTGATGACATTGAAGCTGAAGCCAACTCTTACTTCCATCAAATGTTTTCTGGTCAGTTGACTATTGAATCAATGGTCCAAATGCTTGCCCGTTTCAAAGAATCTTCTGTTCAAAGGGAACAATCCATTTTTGAGTGCATGATTGCTAATCTGTTTGAAGAGTACAGATTCTTTCCGAAATATCCTGAAAGACAGCTCAAAATTGCCGCAATTTTATTTGGGTCTGTCATCAAGCAGCAGCTTGTAACTCATCTGACTCTAGGGATTGCTCTGCGTGGTGTTTTAGATGCATTGCGCAAACCTGCGGATTCAAAAATGTTTGTGTTTGGAACAAAGGCTTTGGAGCAGTTTGTGGAGCGTCTGATTGAATGGCCACAGTACTGCAATCACATTCTTCAGATATCCCATTTGCGCAATACTCATTCAGAGCTGGTGGTATTCATTGAACAGGCTCTTGCCAGGATTTCATCCGGTCATTCAGATTCAGATGGAAGTAACCATGCTTCTGCCGTTCATCATCACGGTCCCAGTCAAGTTACCTCAGGAAATGTGGAGTTAAATGGATCAAGCATCTTACAAACTGGGCAGACACTATCTTCTCCGCTCCAGCTCCATCAGAGACATGAAAGCTCTTTTGATGACCGTCACAAGTCCTCAACTCCATCTAATGAGGTAAAGCCACTTTTATCTTCTGCGACACAACCTGCAGTTGTTCCTGTTAGTGACGCTCCGAGTATTCAGAAGTTACAAAATGCAGTTAGTGCTCCATCAATGTTGTCTTCTGCTTCCCCTGGTTTTGTTCGTCCGTCACGAGGAGCTACCTCTACTAGGTTTGGCTCTGCTTTGAGCATTGAAACATTGGTTGCAGCAGCTGAGAAAAGAGATGCTCCCGTTGAGGCTCCAGCAGAGATTCAGGACAAAGTATTATTTATAGTTAATAATATATCACTCGCTAATATTGAAGTTAAGTCGAAGGAGTTTTCTGAGATACTGAAAGAGCAATACTATCCCTGGTTTGCACAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCCAATTTTCATGACTTGTACCTGAAATTTCTGGACAAACTTAATTCGAAGGCTTTGAATAAAGAGATTGTTCAAGCCACGTATGAGAACTGCAAGGTTCTTCTAGGGTCTGAGCTTATAAAATCAAGCTCAGAGGAGCGTTCATTGCTAAAAAATTTAGGTAGCTGGCTTGGGAAGCTTACTATTGGAAGGAATCAAGTCTTAAGAGCTCGTGAAATTGATCCCAAATCTTTGATCATAGAGGCATATGAGAAAGGGTTGATGATTGCTGTCATACCTTTTACTTCAAAGATTTTGGAACCATGTCAGAATAGCCTCGCGTATCAACCACCGAATCCCTGGACAATGGGTATTCTTGGATTACTTGCTGAGATCTATTCCATGCCAAACTTGAAGATGAACCTCAAGTTTGATATAGAGGTTCTATTCAAGAATCTAGGAGTGGATTTGAAGGAGATAACACCAACTTCCCTTCTCAAGGATCGCAACAGAGAACTTGAAGGGAATCCAGATTTCTCCAACAAAGATGTTGGAGCATCTCAACCACAGATTGCTGAAGTTAAATCTGGAATTATTTCTCCACTAAACCAAGTTGAGCTGCCACTTGACGTTGCTCCATCTAATTCTGGGAGCCATACACATCTGTTATCTCAGTATGCTACCTTGATGGAGGATGACAAGCTTGCTTTGGGGTTATCTGATCAGATCACTTCTGCTCAAGGACTGTTACAAGCTACTCCATCACAGTCCCCATTTTCTGTTAGTCAGCATCCCACAGCAATACCTAATATTGGAACCCACGTAATTATCAACCAGAAGCTCAGTGCCTTGGGGGGCCTGCATGTGCATTTCCAAAGAGTGGTTCCAATTGCTATGGATAGAGCTATCAAGGAAATTGTCTCTGGTATTGTTCAGCGCAGTGTTTCTATAGCTACCCAGACAACTAAGGAGCTTGTTTTAAAGGACTATGCCATGGAATCAGATGACACAAGAATTTTTACTGCAGCGCACTTGATGGTCGCCAGTTTGGCAGGAAGTCTAGCGCATGTCACATGCAAGGAACCGTTGCGCAGTTCAATATTAAGTCAGCTAAGAAATTCTCTTCAGGGATTAAACATCGCAAGTGAACTTCTAGAACAAGCTGTTCAACTTGTAACCAATGATAATCTGGATCTTGGCTGTGCAGTCATTGAGCAGGCTGCTACAGATAAGGCGGTAGCAACAATTGACGGGGAAATAGGTCCGCAGCTTTCAGTAAGAAGAAAGAGAGAAGGTGTTGGTGCAACCTATTTTGATGCAAACATGTATCCACAAGGTTCTATGGGTGTTGTGCCAGAAGCTCTCCGCCCTAAACCTGGTCATTTGTCCCTTTCTCAGCAACGAGTTTATGAGGATTTTGTTCGGCTTCCGTGGCAAAATCAATCTATTCAAAGTTCGCACGTTCTGCCAGTTGCTACCACAGCTTCATCTGGTAGTGCTGGTCTCACTAGCACATATGGTTCAGCATCCGGACAACTTAACACAGGCTACTCACCAGGACCTGGAAGCAAATTTGAAGCCGTTTCTCGTCCATTGGATGAAGCGATGGAATCCAATTCTGCCCTCCATCTTAGTGCTTCATCTATTCATGTTGGGGCGGCTGATGGTGTTTCCCAGCATAGTTCTGAAAATGATTCTGTTGTTGGCTCATTTCCTTCTGCTGCTTCTGCCCCTGAGCTGCACTCTGTAGAGTCATCTGATGCTGTAAAGGAATCTGGAGTGTCGTCACAGCCACCACCTTCACCTGCTGTAACTGAACGTCTTGGAAGTCTCTCAGAACCTTCCCTCACCACAGGGGCTGCATTGGAAAAATACCAGATTGTTGCACAGAAGGTTCCAGAACTGTTGTGTTTGGTCCCTGTCTGGCGTATCATTCATTTTCATGTATATAACATTACCCAAATTGGGTTGGCACTCTTTATCCGCTTCTATTTTGAGAGGGGGGATCGGAGAAGGGGACAAAGGCATTGCTCAGCCAAACACTTCAAGCCAGCAACAGAGTTCGCCACTTCCTTGGTCCGAACTTTGGTGAATGAAGAATATAAAGTTATCTCAGAACTTCAGAGTCTTGTTGATGCAATGGGAAAGGCTTCTGTTCACTCCCCAGTAAGCAGGGAAGAGTTCAGTATTGTGGAGTCTGTGGAACCAGATCCTCCTGGTTTTCGGGAGCAGGTGTCTATGCTGTTTGCTGAGTGGTACCGGATTTGTGAACTTCCTGGTGCAAACGAAAATAGTTGTGCCCATTTAATCATACAGTTGCACTCAAGTGGCATGCTGAAGGGGGATGATCTTACGGACCGATTTTTCCGACTACTCACAGAGCTTACTGTTGCACATTGTCTATCTTCTGAGGTGATTAATTCAGGCACACTGCAAACTCCTCAACAAGTGCAGAATCTATCCTTCCTTGCCATTGATATTTATGCTAAACTTGTATTTTTAATTCTCAAGATGGAGCAGGGATCAAATAAACTAATTCTCTTGTCCAAGATTTTGGCAGTTACAGTGAGATTCATTCAAAAGGATGCAGAGGAGAAGAAATCATCTTTCAATGCAAGACCGTATTTTAGATTGTTTGTTAACTGGTTGCTTGACCTTGGGTCTTTGGATCCTGTTATTGATGGTGCTAACTTTCAGATATTGACAGCCTTCGCAAATGCATTTCATGCGTTGCAACCCCTCAAAGTTCCTACATTCAGCTTTGCATGGCTCGAGTTGGTGAGTCATAGGAGTTTCATGCCTAAAATGCTAGCTGGAAATGGTCAGAAGGGTTGGCCCTATATCCAACGTTTGCTGGTTGACTTATTTCAATTCATGGAGCCTTTTTTGAGGAATGCTGAACTAGGAGTTCCGGTTCACTTCCTGTATAAAGGCACCCTTAGAGTGTTACTTGTGCTGCTCCATGATTTCCCCGAGTTCCTTTGTGATTATCACTTCACTTTCTGTGATGTGATTCCCCCAAGTTGCATTCAAATGCGAAATATCATCCTCAGTGCGTTTCCCCGTAACATGCGGCTTCCTGACCCATCTACTCCGAACTTAAAGATTGATTTGCTTGCTGAAATCACTCAATCTCCACGTGTTCTTTCTGAGGTTGATGCAGCTCTGAAATTAAAGCAGATGAAGACCGATGTGGATGAGTATCTCAAGACAAGGCAACAAGGTTCTTCATTTTTGACCGAATTGAAGCAAAAATTGCTCCTTTTACCAACCGAAGCTGCCTCAGCTGGTACTCGTTACAATGTACCCCTCATCAACTCCCTTGTACTCTATGTGGGGATGCAGGCTATCCAGCAGCTTCAGGCTAGAACACCTCATGCACAGGCAAATCAAACTGTTCCATTTGTATATTTGGTGGGTGCTGCTTTGGATATCTTCCAGACTTTGATAGTGGACCTAGATACTGAAGGACGCTACCTTTTCCTAAATGCAATTGCAAACCAACTGCGCTATCCGAACACCCATACACATTACTTCTCCTTCATTGTTCTTTACTTGTTTGCGGAGGCAAACCAGCAGGAAATTATCCAGGAGCAAATCACAAGGGTATTGTTGGAACGTCTCATTGTTAATCGCCCTCATCCATGGGGTCTACTGATAACATTCATTGAGCTAATCAAGAATCCGAGGTACAACTTCTGGAACCGGGCTTTCATAAGGTGTGCACCAGAGATTGAAAAACTTTTTGAATCAGTATCGAGGTCTTGTGGCGCAAAGCCTGTGGATGAGAGTATGGTTTCAGGTTGGGTACCTGAGACCGCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2201

Amino Acids

244.83

Weight (kDa)

5.71

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 398 - 539 1.5e-33 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 574 - 736 2.2e-67 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 880 - 1097 5.4e-90 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1215 - 1354 5.3e-48 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
Not1 PF04054 1817 - 2177 3.3e-142 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2915
AarI CACCTGC 2 cut(s) 4588, 4996
Acc16I TGCGCA 3 cut(s) 2039, 2156, 3866
Acc36I ACCTGC 5 cut(s) 1173, 2053, 2455, 4588, 4996
Acc65I GGTACC 2 cut(s) 5031, 6583
AccB1I GGYRCC 3 cut(s) 5031, 5708, 6583
AccB7I CCANNNNNTGG 2 cut(s) 3655, 4337
AccBSI CCGCTC 2 cut(s) 2347, 6596
AccI GTMKAC 1 cut(s) 6427
AccII CGCG 1 cut(s) 3116
AccIII TCCGGA 1 cut(s) 4304
AclI AACGTT 1 cut(s) 5623
AcoI YGGCCR 2 cut(s) 982, 2114
AcsI RAATTY 8 cut(s) 52, 216, 751, 2822, 2950, 3789, 3892, 4346
AcvI CACGTG 1 cut(s) 5915
AflII CTTAAG 2 cut(s) 1343, 2998
AflIII ACRYGT 2 cut(s) 4098, 5914
AhdI GACNNNNNGTC 1 cut(s) 5434
AjuI GAANNNNNNNTTGG 4 cut(s) 1760, 1792, 3642, 3674
AleI CACNNNNGTG 1 cut(s) 5100
AloI GAACNNNNNNTCC 2 cut(s) 5673, 5705
Alw21I GWGCWC 3 cut(s) 2509, 3007, 6502
Alw26I GTCTC 7 cut(s) 1573, 2356, 3700, 4283, 4610, 6401, 6584
Alw44I GTGCAC 1 cut(s) 6498
AlwNI CAGNNNCTG 3 cut(s) 2615, 4337, 4634
Ama87I CYCGRG 3 cut(s) 1433, 5546, 5747
Aor13HI TCCGGA 1 cut(s) 4304
AoxI GGCC 6 cut(s) 982, 1401, 1715, 2114, 3631, 5609
ApaLI GTGCAC 1 cut(s) 6498
ApoI RAATTY 8 cut(s) 52, 216, 751, 2822, 2950, 3789, 3892, 4346
AseI ATTAAT 2 cut(s) 1149, 5193
Asp700I GAANNNNTTC 2 cut(s) 3370, 4924
Asp718I GGTACC 2 cut(s) 5031, 6583
AspLEI GCGC 9 cut(s) 637, 2040, 2157, 3714, 3805, 3841, 3867, 6285, 6548
AsuC2I CCSGG 1 cut(s) 6482
AsuII TTCGAA 1 cut(s) 2843
AsuNHI GCTAGC 1 cut(s) 5584
AvaI CYCGRG 3 cut(s) 1433, 5546, 5747
AvaII GGWCC 9 cut(s) 1524, 1819, 2267, 4037, 4334, 4690, 4852, 5131, 6229
AxyI CCTNAGG 1 cut(s) 2284
BaeGI GKGCMC 2 cut(s) 5077, 6502
BaeI ACNNNNGTAYC 4 cut(s) 1082, 1082, 1115, 1115
BalI TGGCCA 2 cut(s) 984, 2116
BamHI GGATCC 1 cut(s) 5443
BanI GGYRCC 3 cut(s) 5031, 5708, 6583
BanII GRGCYC 2 cut(s) 2799, 3007
BarI GAAGNNNNNNTAC 2 cut(s) 2230, 2262
BauI CACGAG 3 cut(s) 761, 2553, 3006
BbrPI CACGTG 1 cut(s) 5915
BbsI GAAGAC 2 cut(s) 2514, 5966
Bbv12I GWGCWC 3 cut(s) 2509, 3007, 6502
BbvCI CCTCAGC 1 cut(s) 6058
BceAI ACGGC 2 cut(s) 2237, 4340
BciVI GTATCC 4 cut(s) 694, 964, 1110, 4113
BclI TGATCA 2 cut(s) 3030, 3496
BcnI CCSGG 1 cut(s) 6482
BcoDI GTCTC 7 cut(s) 1573, 2356, 3700, 4283, 4610, 6401, 6584
BfmI CTRYAG 8 cut(s) 299, 1703, 1719, 2448, 3723, 3798, 3999, 4521
BfoI RGCGCY 1 cut(s) 638
BfrI CTTAAG 2 cut(s) 1343, 2998
BfuAI ACCTGC 5 cut(s) 1173, 2053, 2455, 4588, 4996
BfuI GTATCC 4 cut(s) 694, 964, 1110, 4113
BglI GCCNNNNNGGC 1 cut(s) 3824
BglII AGATCT 1 cut(s) 3168
BlpI GCTNAGC 1 cut(s) 4807
BmcAI AGTACT 2 cut(s) 649, 2123
Bme18I GGWCC 9 cut(s) 1524, 1819, 2267, 4037, 4334, 4690, 4852, 5131, 6229
BmeRI GACNNNNNGTC 1 cut(s) 5434
BmeT110I CYCGRG 3 cut(s) 1433, 5546, 5747
BmrI ACTGGG 3 cut(s) 2265, 2334, 4934
BmtI GCTAGC 1 cut(s) 5588
BmuI ACTGGG 3 cut(s) 2265, 2334, 4934
BpiI GAAGAC 2 cut(s) 2514, 5966
BplI GAGNNNNNCTC 6 cut(s) 41, 73, 779, 811, 4502, 4534
BpmI CTGGAG 5 cut(s) 705, 1539, 2333, 2628, 4572
Bpu10I CCTNAGC 2 cut(s) 4509, 6058
Bpu1102I GCTNAGC 1 cut(s) 4807
Bpu14I TTCGAA 1 cut(s) 2843
BpuMI CCSGG 1 cut(s) 6482
Bsa29I ATCGAT 1 cut(s) 1088
BsaAI YACGTR 3 cut(s) 2877, 3598, 5915
BsaBI GATNNNNATC 3 cut(s) 551, 1692, 5976
BsaI GGTCTC 2 cut(s) 4283, 6584
BsaWI WCCGGW 5 cut(s) 627, 2213, 4304, 5034, 5686
BsaXI ACNNNNNCTCC 6 cut(s) 4994, 5024, 5673, 5703, 5893, 5923
Bse1I ACTGG 8 cut(s) 737, 1401, 2271, 2329, 3819, 4244, 4940, 5423
Bse21I CCTNAGG 1 cut(s) 2284
Bse3DI GCAATG 5 cut(s) 338, 2033, 2939, 4801, 4920
Bse8I GATNNNNATC 3 cut(s) 551, 1692, 5976
BseAI TCCGGA 1 cut(s) 4304
BseCI ATCGAT 1 cut(s) 1088
BseJI GATNNNNATC 3 cut(s) 551, 1692, 5976
BseMI GCAATG 5 cut(s) 338, 2033, 2939, 4801, 4920
BseNI ACTGG 8 cut(s) 737, 1401, 2271, 2329, 3819, 4244, 4940, 5423
BseRI GAGGAG 6 cut(s) 1499, 1670, 2571, 2945, 5205, 5381
BseSI GKGCMC 2 cut(s) 5077, 6502
BseYI CCCAGC 1 cut(s) 4449
BsgI GTGCAG 4 cut(s) 565, 3999, 4499, 5246
Bsh1236I CGCG 1 cut(s) 3116
Bsh1285I CGRYCG 1 cut(s) 1088
BshFI GGCC 6 cut(s) 984, 1403, 1717, 2116, 3633, 5611
BshNI GGYRCC 3 cut(s) 5031, 5708, 6583
BshVI ATCGAT 1 cut(s) 1088
BsiEI CGRYCG 1 cut(s) 1088
BsiHKAI GWGCWC 3 cut(s) 2509, 3007, 6502
BsiHKCI CYCGRG 3 cut(s) 1433, 5546, 5747
BsiSI CCGG 6 cut(s) 628, 2214, 4305, 5035, 5687, 6481
BslFI GGGAC 8 cut(s) 897, 1537, 1598, 2253, 3529, 4147, 4676, 4806
BsmAI GTCTC 7 cut(s) 1573, 2356, 3700, 4283, 4610, 6401, 6584
BsmBI CGTCTC 1 cut(s) 6401
BsmFI GGGAC 8 cut(s) 897, 1537, 1598, 2253, 3529, 4147, 4676, 4806
BsmI GAATGC 3 cut(s) 791, 5674, 5802
BsnI GGCC 6 cut(s) 984, 1403, 1717, 2116, 3633, 5611
Bso31I GGTCTC 2 cut(s) 4283, 6584
BsoBI CYCGRG 3 cut(s) 1433, 5546, 5747
Bsp119I TTCGAA 1 cut(s) 2843
Bsp1286I GDGCHC 5 cut(s) 2509, 2799, 3007, 5077, 6502
Bsp13I TCCGGA 1 cut(s) 4304
Bsp1720I GCTNAGC 1 cut(s) 4807
Bsp19I CCATGG 2 cut(s) 3768, 6419
BspANI GGCC 6 cut(s) 984, 1403, 1717, 2116, 3633, 5611
BspDI ATCGAT 1 cut(s) 1088
BspEI TCCGGA 1 cut(s) 4304
BspFNI CGCG 1 cut(s) 3116
BspHI TCATGA 1 cut(s) 2806
BspMAI CTGCAG 4 cut(s) 303, 1707, 2452, 3802
BspMI ACCTGC 5 cut(s) 1173, 2053, 2455, 4588, 4996
BspOI GCTAGC 1 cut(s) 5588
BspT104I TTCGAA 1 cut(s) 2843
BspT107I GGYRCC 3 cut(s) 5031, 5708, 6583
BspTI CTTAAG 2 cut(s) 1343, 2998
BspTNI GGTCTC 2 cut(s) 4283, 6584
BsrBI CCGCTC 2 cut(s) 2347, 6596
BsrDI GCAATG 5 cut(s) 338, 2033, 2939, 4801, 4920
BsrI ACTGG 8 cut(s) 737, 1401, 2271, 2329, 3819, 4244, 4940, 5423
BssSI CACGAG 3 cut(s) 761, 2553, 3006
BssT1I CCWWGG 6 cut(s) 1697, 3624, 3768, 4848, 5431, 6419
Bst2BI CACGAG 3 cut(s) 761, 2553, 3006
Bst6I CTCTTC 5 cut(s) 211, 1110, 1896, 3903, 4947
BstAFI CTTAAG 2 cut(s) 1343, 2998
BstAPI GCANNNNNTGC 5 cut(s) 1013, 2504, 4240, 5501, 6031
BstBAI YACGTR 3 cut(s) 2877, 3598, 5915
BstBI TTCGAA 1 cut(s) 2843
BstDSI CCRYGG 3 cut(s) 3768, 4205, 6419
BstENI CCTNNNNNAGG 3 cut(s) 1652, 2569, 3272
BstFNI CGCG 1 cut(s) 3116
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 9 cut(s) 637, 2040, 2157, 3714, 3805, 3841, 3867, 6285, 6548
BstMAI GTCTC 7 cut(s) 1573, 2356, 3700, 4283, 4610, 6401, 6584
BstMCI CGRYCG 1 cut(s) 1088
BstSFI CTRYAG 8 cut(s) 299, 1703, 1719, 2448, 3723, 3798, 3999, 4521
BstSLI GKGCMC 2 cut(s) 5077, 6502
BstUI CGCG 1 cut(s) 3116
BstV2I GAAGAC 2 cut(s) 2514, 5966
BstX2I RGATCY 5 cut(s) 201, 3168, 3967, 4984, 5443
BstXI CCANNNNNNTGG 4 cut(s) 1324, 1559, 4849, 5329
BstYI RGATCY 5 cut(s) 201, 3168, 3967, 4984, 5443
Bsu15I ATCGAT 1 cut(s) 1088
Bsu36I CCTNAGG 1 cut(s) 2284
BsuI GTATCC 4 cut(s) 694, 964, 1110, 4113
BsuRI GGCC 6 cut(s) 984, 1403, 1717, 2116, 3633, 5611
BsuTUI ATCGAT 1 cut(s) 1088
BtgI CCRYGG 3 cut(s) 3768, 4205, 6419
BtgZI GCGATG 2 cut(s) 3898, 4394
BtsI GCAGTG 2 cut(s) 3721, 5204
BtsIMutI CAGTG 9 cut(s) 14, 512, 1111, 3625, 3721, 5204, 5346, 5833, 6598
BveI ACCTGC 5 cut(s) 1173, 2053, 2455, 4588, 4996
CaiI CAGNNNCTG 3 cut(s) 2615, 4337, 4634
CciI TCATGA 1 cut(s) 2806
CfoI GCGC 9 cut(s) 637, 2040, 2157, 3714, 3805, 3841, 3867, 6285, 6548
ClaI ATCGAT 1 cut(s) 1088
CpoI CGGWCCG 1 cut(s) 5131
CseI GACGC 3 cut(s) 2090, 2477, 6255
CsiI ACCWGGT 1 cut(s) 4150
CspCI CAANNNNNGTGG 2 cut(s) 6537, 6572
CspI CGGWCCG 1 cut(s) 5131
DraI TTTAAA 1 cut(s) 3756
DriI GACNNNNNGTC 1 cut(s) 5434
EaeI YGGCCR 2 cut(s) 982, 2114
Eam1104I CTCTTC 5 cut(s) 211, 1110, 1896, 3903, 4947
Eam1105I GACNNNNNGTC 1 cut(s) 5434
EarI CTCTTC 5 cut(s) 211, 1110, 1896, 3903, 4947
EciI GGCGGA 1 cut(s) 4130
Ecl136II GAGCTC 1 cut(s) 3005
Eco130I CCWWGG 6 cut(s) 1697, 3624, 3768, 4848, 5431, 6419
Eco24I GRGCYC 2 cut(s) 2799, 3007
Eco31I GGTCTC 2 cut(s) 4283, 6584
Eco32I GATATC 3 cut(s) 1330, 2145, 6208
Eco47I GGWCC 9 cut(s) 1524, 1819, 2267, 4037, 4334, 4690, 4852, 5131, 6229
Eco53kI GAGCTC 1 cut(s) 3005
Eco72I CACGTG 1 cut(s) 5915
Eco81I CCTNAGG 1 cut(s) 2284
Eco88I CYCGRG 3 cut(s) 1433, 5546, 5747
EcoICRI GAGCTC 1 cut(s) 3005
EcoNI CCTNNNNNAGG 3 cut(s) 1652, 2569, 3272
EcoO109I RGGNCCY 3 cut(s) 1524, 3631, 4334
EcoRV GATATC 3 cut(s) 1330, 2145, 6208
EcoT14I CCWWGG 6 cut(s) 1697, 3624, 3768, 4848, 5431, 6419
EcoT22I ATGCAT 3 cut(s) 1018, 2035, 5497
EcoT38I GRGCYC 2 cut(s) 2799, 3007
ErhI CCWWGG 6 cut(s) 1697, 3624, 3768, 4848, 5431, 6419
Esp3I CGTCTC 1 cut(s) 6401
FalI AAGNNNNNCTT 4 cut(s) 1113, 1145, 5697, 5729
FaqI GGGAC 8 cut(s) 897, 1537, 1598, 2253, 3529, 4147, 4676, 4806
FauI CCCGC 1 cut(s) 1266
FauNDI CATATG 2 cut(s) 3043, 4291
FbaI TGATCA 2 cut(s) 3030, 3496
FblI GTMKAC 1 cut(s) 6427
FriOI GRGCYC 2 cut(s) 2799, 3007
FspI TGCGCA 3 cut(s) 2039, 2156, 3866
GlaI GCGC 9 cut(s) 636, 2039, 2156, 3713, 3804, 3840, 3866, 6284, 6547
GsaI CCCAGC 1 cut(s) 4453
GsuI CTGGAG 5 cut(s) 705, 1539, 2333, 2628, 4572
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 6 cut(s) 984, 1403, 1717, 2116, 3633, 5611
HapII CCGG 6 cut(s) 628, 2214, 4305, 5035, 5687, 6481
HgaI GACGC 3 cut(s) 2090, 2477, 6255
HhaI GCGC 9 cut(s) 637, 2040, 2157, 3714, 3805, 3841, 3867, 6285, 6548
Hin6I GCGC 9 cut(s) 635, 2038, 2155, 3712, 3803, 3839, 3865, 6283, 6546
HinP1I GCGC 9 cut(s) 635, 2038, 2155, 3712, 3803, 3839, 3865, 6283, 6546
HincII GTYRAC 4 cut(s) 196, 1803, 5416, 5635
HindII GTYRAC 4 cut(s) 196, 1803, 5416, 5635
HindIII AAGCTT 2 cut(s) 2972, 3476
HpaI GTTAAC 1 cut(s) 5416
HpaII CCGG 6 cut(s) 628, 2214, 4305, 5035, 5687, 6481
HpyCH4IV ACGT 8 cut(s) 2876, 3405, 3597, 4236, 4594, 5623, 5914, 6394
HpySE526I ACGT 8 cut(s) 2876, 3405, 3597, 4236, 4594, 5623, 5914, 6394
HspAI GCGC 9 cut(s) 635, 2038, 2155, 3712, 3803, 3839, 3865, 6283, 6546
KflI GGGWCCC 1 cut(s) 1524
Kpn2I TCCGGA 1 cut(s) 4304
KpnI GGTACC 2 cut(s) 5035, 6587
Ksp22I TGATCA 2 cut(s) 3030, 3496
KspAI GTTAAC 1 cut(s) 5416
MabI ACCWGGT 1 cut(s) 4150
MaeII ACGT 8 cut(s) 2876, 3405, 3597, 4236, 4594, 5623, 5914, 6394
MbiI CCGCTC 2 cut(s) 2347, 6596
MfeI CAATTG 3 cut(s) 3663, 4020, 6267
MflI RGATCY 5 cut(s) 201, 3168, 3967, 4984, 5443
MhlI GDGCHC 5 cut(s) 2509, 2799, 3007, 5077, 6502
MlsI TGGCCA 2 cut(s) 984, 2116
MluNI TGGCCA 2 cut(s) 984, 2116
MlyI GAGTC 6 cut(s) 1733, 1993, 4535, 4909, 4979, 5566
MmeI TCCRAC 6 cut(s) 1576, 3307, 3339, 5167, 5644, 6369
Mox20I TGGCCA 2 cut(s) 984, 2116
Mph1103I ATGCAT 3 cut(s) 1018, 2035, 5497
MroI TCCGGA 1 cut(s) 4304
MroXI GAANNNNTTC 2 cut(s) 3370, 4924
MscI TGGCCA 2 cut(s) 984, 2116
MslI CAYNNNNRTG 8 cut(s) 505, 1021, 1367, 1560, 4407, 4716, 5100, 5807
Msp20I TGGCCA 2 cut(s) 984, 2116
MspA1I CMGCKG 5 cut(s) 244, 304, 958, 2615, 6062
MspCI CTTAAG 2 cut(s) 1343, 2998
MspI CCGG 6 cut(s) 628, 2214, 4305, 5035, 5687, 6481
MunI CAATTG 3 cut(s) 3663, 4020, 6267
Mva1269I GAATGC 3 cut(s) 791, 5674, 5802
MvnI CGCG 1 cut(s) 3116
NciI CCSGG 1 cut(s) 6482
NcoI CCATGG 2 cut(s) 3768, 6419
NdeI CATATG 2 cut(s) 3043, 4291
NheI GCTAGC 1 cut(s) 5584
NmuCI GTSAC 6 cut(s) 1049, 2387, 2465, 2550, 3844, 4560
NsbI TGCGCA 3 cut(s) 2039, 2156, 3866
NsiI ATGCAT 3 cut(s) 1018, 2035, 5497
NspV TTCGAA 1 cut(s) 2843
OliI CACNNNNGTG 1 cut(s) 5100
PaeI GCATGC 1 cut(s) 5110
PaeR7I CTCGAG 2 cut(s) 1433, 5546
PagI TCATGA 1 cut(s) 2806
PaqCI CACCTGC 2 cut(s) 4588, 4996
PciI ACATGT 1 cut(s) 4098
PcsI WCGNNNNNNNCGW 1 cut(s) 2551
PctI GAATGC 3 cut(s) 791, 5674, 5802
PdmI GAANNNNTTC 2 cut(s) 3370, 4924
PflMI CCANNNNNTGG 2 cut(s) 3655, 4337
PfoI TCCNGGA 1 cut(s) 6363
Ple19I CGATCG 1 cut(s) 1088
PleI GAGTC 6 cut(s) 1732, 1993, 4534, 4908, 4978, 5565
PmaCI CACGTG 1 cut(s) 5915
PmlI CACGTG 1 cut(s) 5915
PpsI GAGTC 6 cut(s) 1732, 1993, 4534, 4908, 4978, 5565
Ppu21I YACGTR 3 cut(s) 2877, 3598, 5915
PpuMI RGGWCCY 2 cut(s) 1524, 4334
PscI ACATGT 1 cut(s) 4098
PshBI ATTAAT 2 cut(s) 1149, 5193
PsiI TTATAA 1 cut(s) 2915
Psp124BI GAGCTC 1 cut(s) 3007
Psp1406I AACGTT 1 cut(s) 5623
Psp5II RGGWCCY 2 cut(s) 1524, 4334
PspCI CACGTG 1 cut(s) 5915
PspFI CCCAGC 1 cut(s) 4449
PspPPI RGGWCCY 2 cut(s) 1524, 4334
PspXI VCTCGAGB 1 cut(s) 5546
PstI CTGCAG 4 cut(s) 303, 1707, 2452, 3802
PstNI CAGNNNCTG 3 cut(s) 2615, 4337, 4634
PsuI RGATCY 5 cut(s) 201, 3168, 3967, 4984, 5443
PvuI CGATCG 1 cut(s) 1088
PvuII CAGCTG 4 cut(s) 304, 958, 2615, 6062
RseI CAYNNNNRTG 8 cut(s) 505, 1021, 1367, 1560, 4407, 4716, 5100, 5807
Rsr2I CGGWCCG 1 cut(s) 5131
RsrII CGGWCCG 1 cut(s) 5131
SacI GAGCTC 1 cut(s) 3007
ScaI AGTACT 2 cut(s) 649, 2123
SchI GAGTC 6 cut(s) 1733, 1993, 4535, 4909, 4979, 5566
SduI GDGCHC 5 cut(s) 2509, 2799, 3007, 5077, 6502
SexAI ACCWGGT 1 cut(s) 4150
SfcI CTRYAG 8 cut(s) 299, 1703, 1719, 2448, 3723, 3798, 3999, 4521
Sfr274I CTCGAG 2 cut(s) 1433, 5546
SfuI TTCGAA 1 cut(s) 2843
SinI GGWCC 9 cut(s) 1524, 1819, 2267, 4037, 4334, 4690, 4852, 5131, 6229
SlaI CTCGAG 2 cut(s) 1433, 5546
SmiMI CAYNNNNRTG 8 cut(s) 505, 1021, 1367, 1560, 4407, 4716, 5100, 5807
SphI GCATGC 1 cut(s) 5110
SspI AATATT 5 cut(s) 226, 1323, 2701, 3586, 3876
SstI GAGCTC 1 cut(s) 3007
StyI CCWWGG 6 cut(s) 1697, 3624, 3768, 4848, 5431, 6419
TaiI ACGT 8 cut(s) 2879, 3408, 3600, 4239, 4597, 5626, 5917, 6397
TaqI TCGA 8 cut(s) 1084, 1088, 1434, 1604, 2714, 2843, 5547, 6533
TaqII GACCGA 3 cut(s) 5148, 5978, 6029
TatI WGTACW 5 cut(s) 647, 1042, 1905, 2121, 6100
TauI GCSGC 4 cut(s) 1403, 1952, 4436, 5851
TscAI CASTG 8 cut(s) 21, 512, 1118, 3625, 3721, 5211, 5346, 5833
TseFI GTSAC 6 cut(s) 1049, 2387, 2465, 2550, 3844, 4560
Tsp45I GTSAC 6 cut(s) 1049, 2387, 2465, 2550, 3844, 4560
TspGWI ACGGA 6 cut(s) 383, 983, 1348, 2538, 4194, 5144
TspRI CASTG 8 cut(s) 21, 512, 1118, 3625, 3721, 5211, 5346, 5833
Van91I CCANNNNNTGG 2 cut(s) 3655, 4337
Vha464I CTTAAG 2 cut(s) 1343, 2998
VneI GTGCAC 1 cut(s) 6498
VpaK11BI GGWCC 9 cut(s) 1524, 1819, 2267, 4037, 4334, 4690, 4852, 5131, 6229
VspI ATTAAT 2 cut(s) 1149, 5193
XagI CCTNNNNNAGG 3 cut(s) 1652, 2569, 3272
XapI RAATTY 8 cut(s) 52, 216, 751, 2822, 2950, 3789, 3892, 4346
XbaI TCTAGA 2 cut(s) 1027, 3928
XcmI CCANNNNNNNNNTGG 3 cut(s) 729, 3132, 3961
XhoI CTCGAG 2 cut(s) 1433, 5546
XmiI GTMKAC 1 cut(s) 6427
XmnI GAANNNNTTC 2 cut(s) 3370, 4924
ZrmI AGTACT 2 cut(s) 649, 2123
Zsp2I ATGCAT 3 cut(s) 1018, 2035, 5497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.