MD07G1112500.v1.1

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005282
Physical Location & Seq
Forward (+)
13145771 .. 13164480
18710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1112500.v1.1.491

Sequence Viewer

Length: 7155 bp
ATGCTCAATTTTACGCCGACCACCGCGAGCCAGATTCGCTTCCTACTCCAGAGCCTCAACGATGCCAACTCGGACTCCGTTCTCCGAGAGCTCTCTCAGTTTACTGAACATGGACTTGAGGGAAGCATTTTGCTGCTACAGACCTGTCTGGATCATCTCACTAATTACAGAACAGACTTAAAGAGCGCAGCATTGGAACGAGTTCTTGCCTCAATTTTCAAATATCTCCTTGACAAACCTAATTTCAGTACGGTGTTTTGTGAGTGCCTAAGAAATATAGAGATTAATGAAGGAATTCTTGAGAATTTCTCTAATGCATTACTCCTGTCTGTATCGGAGAAAATTAGCATTGCTCTTGCGCTGTCAGATTCCGAAGACCTAGACACCAGGATATGTGGCAAGAACTTCTGTATGGATCAGATCCAGAAATTGTGTGAAAGTCCTATTGCTATGAATTCGTCTGAGCAAATTCAGAACATTGTTATATTCCTCCAGCGGTCTGAGGGCCGTTCCAAGCATGTGGATTCCTTTATGCAGATGTTATCTTTACTGCAGCCGAAAGATGACAGTTTTTCTGTTTTAACTCCATTGCTTTCAGATGAGCTCCGTGATGCCAATTTTTTGAGCAATGTGGGTTTGTTGCATGAAAGCGGAGAAAATGATTTTGATGCTATTTTAGCTGAAATGGCGAAAGAAATCAGCATGGGAGATGTAATGAAGGAATTAGGTTATGGATGCACAGTTGATTCATCTCAGTGCAAAGAGATTTTATCTCTTTTCTTACCACTCACTGAGATTACTATTTCAAAAATACTTGGCATGATTGCCTCTACTCATGTTGGTCTCGAGGACAATCAGAATGCATTTTCAACATTCCGGTTGGCGGTTGGTAACAGCACTCTATCTGATCTGCCTATGTTGAACACCTGGAATATTGATGTCCTTGTAGATACAATCAAGCAACTTGCTCCCGGTACTAACTGGATACAAGTTATTGAAAATTTGGATCATGAGGGATTCTTCATTCCTAATCAGGACGCATTCTCTTTTTTCATGTCTGTATATCAGCATGTGTGTCAGGAGCCGTTCCCCCTCCATGTTATCTGTGGGTCTGTTTGGAAGAACACCGAGGGTCAGCTATCCTTCCTTAAACATGCTGTGTCAGCACCACCTGAAGTGTTTACCTTTGCCCACTCTGTTAGGCAGCTGGCCTATATTGATGCAGTGCATGGCCATAAGCTTGAACTTGGACATGCAAATCATGCATGGTTGAGTCTTGACCTTTTGGATGTACTGTGTCTACTAGCTGAGAGGGGTCATGCTCTTGCTGTTCGATCGATGCTTGAGTATCCTCTTAAACACTGTCCTGAAGTCCTGCTTCTTGGGATGGCGCATACAAATACTGCTTATAACCTGCTGCAATATGAAGTGTCTTTTACTGTTTTCCCCATGATGGTAAAAAATGCCATGGGCATTGGCATGATTAACCACCTTTGGCATATTAACACCTCACTGGTGCTGCGGGGATTTGTGGATGCTCACAATTGTGATCCAGATAGCATAGCTAGAATATTGGATATCTGTCAAGAGCTAAAGATTCTGTCATCTGTCCTGGAGATGATCCCTTCCTCTTTAAGTATCAGATTAGCAGCCCTTGCTTCTAAAAAAGAGCTTATAGACCTTGAGAAGTGGTTGACTAATAATTTGAATACGTATAAGGATACTTTCTTTGAGGAGTGCGTAAAGTTCTTGAAGGAGATTCAATTTGGTGGTTCACAGGATTTTAACACCAGACCTTTCCAACATTCTGGTGCCGTGTCTAATATTTATGTGGACACTGCTACTATCTTCACGAAGGTTCTTAAAGCTCATGTTGGCTTGGTTGCCTCTAGCCAACTTACTGAGGAGATGGAAAGGTTGTCTGTAACAATTATGGATTCGATTCCAAGGCTGCCGAATGGTGGCACTACAGAGTCTTCAACTGATGGATATGCAGATGATATTGAAGCTGAAGCAAACTCTTACTTCCATCAAATGTTTTCTGGTCAATTGACAATTGATTCAATGGTCCAAATGCTTGCCCGATTCAAGGAATCTTCTGTAAAAAGGGAACAGTCAATTTTTGAGTGTATGATTGCAAATCTGTTTGAAGAGTATAGATTCTTCCCCAAGTATCCTGAAAGACAGCTCAAAATTGCTGCAGTTCTGTTTGGCTCTGTCATCAAGCACCAGCTCGTAACTCATCTGACTCTTGGGATTGCTCTGCGTGGAGTTTTAGATGCATTGCGCAAACCTGCAGATTCAAAAATGTTTGTGTTCGGAACTAAGGCCTTGGAGCAGTTTGTGGATCGTCTGATTGAGTGGCCGCAGTATTGCAATCATATTCTTCAAATATCCCATCTGCGGAGTACTCATTCAGAGCTTGTGGCTTTCATTGAACAGGCTCTTGCCAAGATTTCTTCAAGCCATTCAGACTCGGATGGAAGTAACCATGCTTCTGCTGCTCATCACCATGGTCCCAGTCAAGCTACCCCAGGAAATGTGGAGTTAAATGGTTCTACCATCTTACATACCGGGCAACAACATTCTTCTCCACTCCAACTTCAACAGAGACATGAAGGTTCTCTTGATGACCGTCATAAACCCTCAAATTTATCAAATGACATAAAGCCACCTTTATCTTCTGCTGTGCAACCTCCGGTTGGTCCTTCACAAAGTGCACTCAGTGCTCCGGCAATGTTGTCTTCTCCTTCCCCTGGTTTCGTTCGTCCTACACGAGGAGTTACTTCTACCAGGTTTGGCTCTGCTTTGAATATTGAAACATTAGTTGCTGCTGCTGAGAAAAGAGATACTCTCATTGAGGCTCCAGCATCAGAGATTCAGGACAAGATATCATTTATAATTAATAATATTTCAGTTGCCAATGTTGAAGCTAAGTCAAAGGAGTTTGCTGAAATAATGAAAGAGCAGTACTATCCTTGGTTTGCACAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCGAATTTTCATGATTTGTACCTGAAATTTCTGGACAAAGTTAATTCTAAGGGTTTGAATAAGGAGATTGTTCAAGCCACTTATGAGAACTGCAAGGTTCTTCTTGGATCTGAGCTTATAAAGTCAAGCGCAGAAGAGCGTTCGTTGCTTAAAAATTTAGGTAGCTGGCTTGGGAAGCTTACAATTGGAAGGAATCAAGTCTTAAGGGCTCGGGAAATAGATCCCAAATCTTTGATTATAGAGGCATATGAGAAAGGGTTGATGATTGCAGTTATACCCTTTACTTCAAAGATTCTGGAGCCATGCCAAAGTAGCTTGGCATATCAGCCACCAAATCCTTGGACAATGGGTATTCTTGGACTACTTGCTGAGATATATTCAATGCCAAACTTGAAAATGAACCTGAAGTTTGACATCGAGGTTCTATTCAAGAACCTTGGTGTGGATTTGAAGGAGATAACCCCAAGTTCTCTACTCAAGGACCGGAACAGAGAAGTTGAAGGGAATCCTGATTTCTCTAACAAAGATATTGGAGCACCCCAACCACAGATGGTTGCCGAAGTGAAACTAAATCAAGTTGAACTACCAGTTGATGTTGCTCCATCTAATTCTGCAAGCCATACACATATATCTCAGTATGCGACCCCTGTTCATGTTTCACCTGGAACCTTAATCGAGGATGAAAAGGTGTTTCCTGATCAGATTCCTGCTCAAGGACTGTTACCAGCTACTCCATCCCAGTCACCATTCTCTCTTCCCACACCAATATCTAATATTGCAACTCACGTGGTTATCAACCCGAAACTCAGTGCTTTGGGATTGCACTTGCATTTTCAGAGGGTGGTTCCAATTGCAATGGACAGAGCTATCAAAGAGATAGTAACTGGTATTGTTCAGCGCAGTGTTTCTATAGCTACGCAGACAACTAAAGAGCTTGTCTTAAAGGATTATGCCATGGAATCTGATGAGACAAGAATATTACTGGCAGCTCACTTGATGGTTGCAAGTTTGGCAGGAAGTCTAGCTCATGTAACTTGCAAGGAACCCTTGCGTAGTTCAATATCAACCCAGCTGCGGAATTCCCTTCAGGGATTAAATATTCCAAATGATCATCTGGAACATGCTGTACAACTTGTTACTAATGACAATCTGGATCTTGGCTGTGCAATCATTGAACAGTCTGCTACTGATAAGGCAGTACAAACAATTGATGGAGAAATAGCTCAACAACTTTCATTAAGAAGGAAGAGGGAAGGTGCGGGTACAACGTTTTTTGATGCTACCATGTATACACAAGGAGGCAGTGTGCCCGAGTCCCTTCGCCCAAAACCTGGTCATTTGTCCATTTCTCAACAACGAGTTTATGAGGACTTTGTGCGGCTTCCCTGGCAAAACCAGTCTAGCCAGAATTCGCATGTTCTTCCTGCTAGTACTGCAGCTACATCTGCAAGTGCAGGTCTAAATAGCACGTTTGGTTCAGCCTCGGCTCAAATTAACCCAGGCTTCTCAGCAGGCCCCGGAAGCAAATTTGATGCTGTTTCTCGTCCACTGGATGAGGCGATTGAATCCAATCCAGCTCTGCATCCAAGTGCATCCTCTATTAATGTTGGAGCGGGTGATGGTGTTTCCCAGCATAGTTCTGAGAATGATTCTGTCATTGGTTCATTTCCATCTGCTGCTTCTGCCCTAGAGCTGCAATCAGTAGAGTCATCTGATACTATACAGGAGTCTGGAATTTCTTCACAGCCGCAACCATCACCTACTGTAACTGAACGTCTGGCAAGCAACATATCAGAACCTTCTCTCAATACGAGGGATGCATTGGATAAATACCAGATTGTTTCACAGAAGCTTGAAGCTTTGGTAACCAACGATGCTAGAGAGTTAGAAATTCAGGGAGTAATTGGTGAAGTTCCTGAAATTATACTCAGGTGTGTTAGTCGAGATGAGGCTGCCTTGGCTGTGGCTCAAAAGGTTTTCAAGGGTTTATACGAAAATGCATCGAACCATACTCATGTCAGTGCTCATATTGCAATGCTGACAGCTATTCGCGATGTTTGCAAGCTTGTTGTTAAGGAGCTCACTAGTTGGGTTATTTATTCGGATGAGGAGCGGAAGTTTAACAAAGATATTACTGTTGGCCTCATTCACAGTGAATTGCTAAATCTTGCAGAGTACAATGTTCAAATGGCAAAACTTATTGATGGGGGCAGAAATAAGCCTGCAACTGAGTTTTCAATTTCCCTTCTCCAATCTTTGGTGATTGAAGAATCTAAAGTTATATCAGAACTTCATAATCTTGTTGACGCATTGGCAAAGCTCGCTGCAAAGCCTGGATCTCCGGAGTCGCTACAGCAGCTGGTTGAAATGGTAAAGAATCCTGCTTATATTCTTGGTGCGCCATCTGCTATTAATGTAGGAAAAGAGGACAAAGCCAGAATATCTAAAGATAAAAAGGCTTCTGTTCACTCTCCAGTGAGCAGGGAAGACTTTGGTAGTGTGGAGTCTGTCGAACCAGATCCTGCTGGATTCCGTGAGCAGGTGTCTATGCTGTTTGCAGAGTGGTACCGGATTTGCGAACTTCCTGGTGCAAACGATGCAGCTTGTGCCCATTTTATCTTGCAGTTGCATCAAAATGGTCTACTGAAGGGGGATGATATGACAGACCGGTTTTTCCGTGTCCTCACAGAGCTATCTGTTGCACATTGCTTATCTTCTGAGATGACAATTCCAAGCTCACTGCAAACACCTCAACAAGTGCAGAATCTCTCCTTCCTTGCTATTGATATTTATGCTAAACTTGTCTTCTTAATTTTGAAGGGATCAAGTAAACTATTTCTCTTGTCTAAGATTTTGGCAGTCACAGTGAGATTCATTCAAAAGGATGCAGAGGAGAAGAAAACATCTTTCAATCCAAGACCGTATTTTAGATTGTTTGTTAACTGGCTTCTAGACCTTGGTTCTTTGGATCCTGTGATTGATGGTGCTAACTTTCAGATATTGACAGCATTTGCAAATGCATTTCATGCTTTGCAGCCCCTTAAGGTTCCTACGTTCAGCTTTGCATGGCTTGAGTTGGTGAGTCATAGGAGCTTCATGCCAAAAATGCTTGTTGGAAATGGTCAGAAGGGTTGGCCCTATATCCAACGTTTGCTGGTACATTTGTTTCAATTTATGGAGCCATTTTTGAGGAATGCTGAACTTGGAGTTCCGGTTCATTTCATGTATAAAGGCACACTTAGGGTGCTTCTAGTGCTACTTCATGATTTCCCGGAGTTCCTTTGTGATTATCATTTTACTTTCTGTGATGTAATCCCTCCAAGCTGCATTCAAATGCGAAATATCATCCTCAGTGCATTTCCCCGTAATATGAGGCTACCAGATCCATCTACTCCCAATTTAAAGATCGATTTGCTTGCAGAAATCAGTCAGTCTCCACGTATTCTTTCCGAGGTTGATGCAGCTCTAAAAGCGAAGCAGATGAAAGCTGATGTGGATGAGTATCTCAAGACAAGGCAACAGGGTTCTTCATTTCTGACTGAACTGAAGCAAAAGTTGCTCCTTTTACCAAGCGAGGTTGCCTCAGCTGGTACTCGTTACAATGTACCCCTGATCAACTCCCTTGTGCTTTATGTTGGGATGCAGGCTATCCAGCAGCTTCAGGCTAGAACGCCTCATGCGCAATCTACCCAAACTGTTCCATTGACTGTCTATTTGGTGGGTGCGGCTTTGGATATTTTTCAGACTCTGATAGTGGACCTAGATACTGAAGGGCGCTACCTTTTCCTAAATGCGATCGCGAACCAACTGCGTTATCCAAACACCCATACCCATTACTTTTCTTTCATTGTTTTGTACTTGTTTGCTGAATCAAACCAGCATGAAATTATCCAGGAGCAAATCACAAGGGTATTGTTGGAACGTCTGATTGTCAACCGGCCTCATCCATGGGGTCTTCTAATTACCTTCATTGAGCTTATCAAGAATCCGAGATACCAGTTCTGGAACCGAGCTTTCATCAGATGTGCGCCAGAGATTGAAAAACTCTTTGAATCTGTCTCAAGGTCTTGCGGGGGACCAAAACCAGTGGACGAAAGTATGGTCTCAGGTTGGGTATCGGAGAGTGCCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2385

Amino Acids

264.33

Weight (kDa)

5.69

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 479 - 622 1.5e-34 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 657 - 821 1.6e-68 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 953 - 1173 8e-90 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1284 - 1423 2.3e-49 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
ARM_Cnot1 PF25097 1626 - 1800 4.3e-55 Cnot1 ARM repeat
NOT1_connector PF23590 1650 - 1798 3.1e-17 CCR4-NOT transcription complex subunit 1, NOT1_connector
Not1 PF04054 1999 - 2360 2.3e-141 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1410, 2900, 3140
AarI CACCTGC 1 cut(s) 5551
Acc16I TGCGCA 2 cut(s) 2288, 6702
Acc36I ACCTGC 4 cut(s) 1422, 2302, 4466, 5551
Acc65I GGTACC 1 cut(s) 5586
AccB1I GGYRCC 2 cut(s) 1811, 5586
AccB7I CCANNNNNTGG 2 cut(s) 4352, 5278
AccBSI CCGCTC 2 cut(s) 4634, 5134
AccI GTMKAC 3 cut(s) 1300, 4310, 5662
AccII CGCG 3 cut(s) 26, 5073, 6821
AccIII TCCGGA 1 cut(s) 5362
AclI AACGTT 2 cut(s) 4289, 6169
AcoI YGGCCR 2 cut(s) 1231, 2363
AcuI CTGAAG 9 cut(s) 1194, 1389, 2031, 3446, 4091, 5687, 6587, 6665, 6810
AcvI CACGTG 1 cut(s) 3808
AdeI CACNNNGTG 2 cut(s) 2717, 2726
AflII CTTAAG 2 cut(s) 3223, 6062
AgeI ACCGGT 1 cut(s) 5688
AhlI ACTAGT 1 cut(s) 5105
AleI CACNNNNGTG 1 cut(s) 1545
AloI GAACNNNNNNTCC 2 cut(s) 6219, 6251
Alw21I GWGCWC 7 cut(s) 93, 606, 2722, 2731, 3559, 5047, 5103
Alw26I GTCTC 6 cut(s) 848, 2605, 3983, 6459, 7084, 7129
Alw44I GTGCAC 1 cut(s) 2718
AlwNI CAGNNNCTG 3 cut(s) 5380, 5543, 6769
Ama87I CYCGRG 3 cut(s) 845, 3231, 4331
Aor13HI TCCGGA 1 cut(s) 5362
AoxI GGCC 9 cut(s) 505, 1209, 1231, 2328, 2363, 4534, 5161, 6155, 6959
ApaLI GTGCAC 1 cut(s) 2718
ArsI GACNNNNNNTTYG 6 cut(s) 1687, 1719, 6718, 6750, 7107, 7139
AseI ATTAAT 4 cut(s) 285, 2904, 4623, 5433
AsiGI ACCGGT 1 cut(s) 5688
AsiSI GCGATCGC 1 cut(s) 6819
Asp700I GAANNNNTTC 3 cut(s) 201, 294, 4759
Asp718I GGTACC 1 cut(s) 5586
AspS9I GGNCC 9 cut(s) 505, 2068, 2516, 2705, 3502, 4535, 6156, 6778, 7097
AsuC2I CCSGG 4 cut(s) 972, 2575, 4539, 6293
AsuHPI GGTGA 8 cut(s) 2501, 3672, 3756, 4649, 4770, 4940, 5293, 6112
AvaI CYCGRG 3 cut(s) 845, 3231, 4331
AvaII GGWCC 6 cut(s) 2068, 2516, 2705, 3502, 6778, 7097
BaeGI GKGCMC 4 cut(s) 2722, 4332, 5632, 7150
BaeI ACNNNNGTAYC 2 cut(s) 6170, 6203
BalI TGGCCA 1 cut(s) 1233
BamHI GGATCC 1 cut(s) 5989
BanI GGYRCC 2 cut(s) 1811, 5586
BanII GRGCYC 4 cut(s) 93, 606, 3232, 5103
BarI GAAGNNNNNNTAC 4 cut(s) 2147, 2179, 2479, 2511
BauI CACGAG 1 cut(s) 2775
BbrPI CACGTG 1 cut(s) 3808
BbsI GAAGAC 6 cut(s) 381, 1968, 2736, 5514, 5818, 6968
Bbv12I GWGCWC 7 cut(s) 93, 606, 2722, 2731, 3559, 5047, 5103
BbvCI CCTCAGC 1 cut(s) 6604
BceAI ACGGC 3 cut(s) 492, 1069, 1799
BcgI CGANNNNNNTGC 6 cut(s) 6419, 6453, 6461, 6495, 6811, 6845
BciVI GTATCC 4 cut(s) 978, 1359, 1714, 2184
BclI TGATCA 3 cut(s) 3718, 4129, 6633
BcnI CCSGG 4 cut(s) 972, 2575, 4539, 6293
BcoDI GTCTC 6 cut(s) 848, 2605, 3983, 6459, 7084, 7129
BcuI ACTAGT 1 cut(s) 5105
BfmI CTRYAG 8 cut(s) 137, 551, 1968, 2199, 2295, 3930, 4455, 5372
BfoI RGCGCY 1 cut(s) 6799
BfrI CTTAAG 2 cut(s) 3223, 6062
BfuAI ACCTGC 4 cut(s) 1422, 2302, 4466, 5551
BfuI GTATCC 4 cut(s) 978, 1359, 1714, 2184
BmcAI AGTACT 3 cut(s) 2410, 2972, 4453
Bme18I GGWCC 6 cut(s) 2068, 2516, 2705, 3502, 6778, 7097
BmeT110I CYCGRG 3 cut(s) 845, 3231, 4331
BmgT120I GGNCC 9 cut(s) 505, 2068, 2516, 2705, 3502, 4535, 6156, 6778, 7097
BmrI ACTGGG 2 cut(s) 2514, 3754
BmuI ACTGGG 2 cut(s) 2514, 3754
BpiI GAAGAC 6 cut(s) 381, 1968, 2736, 5514, 5818, 6968
BplI GAGNNNNNCTC 6 cut(s) 293, 325, 2838, 2870, 6587, 6619
BpmI CTGGAG 6 cut(s) 32, 476, 1634, 2850, 3338, 5478
Bpu10I CCTNAGC 1 cut(s) 6604
BpuEI CTTGAG 9 cut(s) 137, 320, 1364, 1703, 3482, 3717, 6113, 6512, 7066
BpuMI CCSGG 4 cut(s) 972, 2575, 4539, 6293
Bsa29I ATCGAT 2 cut(s) 1337, 6429
BsaAI YACGTR 3 cut(s) 1713, 3808, 6461
BsaBI GATNNNNATC 1 cut(s) 6522
BsaI GGTCTC 2 cut(s) 848, 7129
BsaWI WCCGGW 7 cut(s) 876, 2698, 3504, 5362, 5589, 5688, 6232
BsaXI ACNNNNNCTCC 8 cut(s) 59, 89, 4311, 4341, 5123, 5153, 6219, 6249
Bse118I RCCGGY 2 cut(s) 5688, 6957
Bse3DI GCAATG 8 cut(s) 348, 587, 634, 2282, 2742, 3882, 5061, 5725
Bse8I GATNNNNATC 1 cut(s) 6522
BseAI TCCGGA 1 cut(s) 5362
BseCI ATCGAT 2 cut(s) 1337, 6429
BseJI GATNNNNATC 1 cut(s) 6522
BseMI GCAATG 8 cut(s) 348, 587, 634, 2282, 2742, 3882, 5061, 5725
BseRI GAGGAG 5 cut(s) 1748, 1919, 2793, 5144, 5927
BseSI GKGCMC 4 cut(s) 2722, 4332, 5632, 7150
BseYI CCCAGC 2 cut(s) 4089, 4650
BsgI GTGCAG 2 cut(s) 4494, 5801
Bsh1236I CGCG 3 cut(s) 26, 5073, 6821
Bsh1285I CGRYCG 2 cut(s) 1337, 6819
BshFI GGCC 9 cut(s) 507, 1211, 1233, 2330, 2365, 4536, 5163, 6157, 6961
BshNI GGYRCC 2 cut(s) 1811, 5586
BshTI ACCGGT 1 cut(s) 5688
BshVI ATCGAT 2 cut(s) 1337, 6429
BsiEI CGRYCG 2 cut(s) 1337, 6819
BsiHKAI GWGCWC 7 cut(s) 93, 606, 2722, 2731, 3559, 5047, 5103
BsiHKCI CYCGRG 3 cut(s) 845, 3231, 4331
BslFI GGGAC 3 cut(s) 2502, 4321, 7110
BsmAI GTCTC 6 cut(s) 848, 2605, 3983, 6459, 7084, 7129
BsmFI GGGAC 3 cut(s) 2502, 4321, 7110
BsmI GAATGC 4 cut(s) 865, 1040, 6220, 6348
BsnI GGCC 9 cut(s) 507, 1211, 1233, 2330, 2365, 4536, 5163, 6157, 6961
Bso31I GGTCTC 2 cut(s) 848, 7129
BsoBI CYCGRG 3 cut(s) 845, 3231, 4331
Bsp13I TCCGGA 1 cut(s) 5362
Bsp1407I TGTACA 1 cut(s) 4147
Bsp19I CCATGG 4 cut(s) 1467, 2512, 3975, 6968
Bsp68I TCGCGA 2 cut(s) 5073, 6821
BspANI GGCC 9 cut(s) 507, 1211, 1233, 2330, 2365, 4536, 5163, 6157, 6961
BspDI ATCGAT 2 cut(s) 1337, 6429
BspEI TCCGGA 1 cut(s) 5362
BspFNI CGCG 3 cut(s) 26, 5073, 6821
BspHI TCATGA 3 cut(s) 1009, 3031, 6283
BspMAI CTGCAG 4 cut(s) 555, 2203, 2299, 4459
BspMI ACCTGC 4 cut(s) 1422, 2302, 4466, 5551
BspQI GCTCTTC 1 cut(s) 3150
BspT107I GGYRCC 2 cut(s) 1811, 5586
BspTI CTTAAG 2 cut(s) 3223, 6062
BspTNI GGTCTC 2 cut(s) 848, 7129
BsrBI CCGCTC 2 cut(s) 4634, 5134
BsrDI GCAATG 8 cut(s) 348, 587, 634, 2282, 2742, 3882, 5061, 5725
BsrFI RCCGGY 2 cut(s) 5688, 6957
BsrGI TGTACA 1 cut(s) 4147
BssAI RCCGGY 2 cut(s) 5688, 6957
BssNAI GTATAC 1 cut(s) 4311
BssSI CACGAG 1 cut(s) 2775
Bst1107I GTATAC 1 cut(s) 4311
Bst2BI CACGAG 1 cut(s) 2775
Bst6I CTCTTC 4 cut(s) 2145, 3150, 3780, 4262
BstAFI CTTAAG 2 cut(s) 3223, 6062
BstAPI GCANNNNNTGC 7 cut(s) 1262, 1655, 2726, 5618, 5627, 6047, 6577
BstAUI TGTACA 1 cut(s) 4147
BstBAI YACGTR 3 cut(s) 1713, 3808, 6461
BstDSI CCRYGG 4 cut(s) 1467, 2512, 3975, 6968
BstEII GGTNACC 1 cut(s) 4885
BstENI CCTNNNNNAGG 2 cut(s) 2775, 3732
BstFNI CGCG 3 cut(s) 26, 5073, 6821
BstH2I RGCGCY 1 cut(s) 6799
BstMAI GTCTC 6 cut(s) 848, 2605, 3983, 6459, 7084, 7129
BstMCI CGRYCG 2 cut(s) 1337, 6819
BstNSI RCATGY 6 cut(s) 521, 1073, 1157, 1256, 4145, 4439
BstPI GGTNACC 1 cut(s) 4885
BstSFI CTRYAG 8 cut(s) 137, 551, 1968, 2199, 2295, 3930, 4455, 5372
BstSLI GKGCMC 4 cut(s) 2722, 4332, 5632, 7150
BstSNI TACGTA 1 cut(s) 1713
BstUI CGCG 3 cut(s) 26, 5073, 6821
BstV2I GAAGAC 6 cut(s) 381, 1968, 2736, 5514, 5818, 6968
BstX2I RGATCY 8 cut(s) 420, 3128, 3241, 4174, 5357, 5539, 5989, 6403
BstXI CCANNNNNNTGG 3 cut(s) 520, 1808, 3360
BstYI RGATCY 8 cut(s) 420, 3128, 3241, 4174, 5357, 5539, 5989, 6403
BstZ17I GTATAC 1 cut(s) 4311
Bsu15I ATCGAT 2 cut(s) 1337, 6429
BsuI GTATCC 4 cut(s) 978, 1359, 1714, 2184
BsuRI GGCC 9 cut(s) 507, 1211, 1233, 2330, 2365, 4536, 5163, 6157, 6961
BsuTUI ATCGAT 2 cut(s) 1337, 6429
BtgI CCRYGG 4 cut(s) 1467, 2512, 3975, 6968
BtgZI GCGATG 1 cut(s) 5088
BtsI GCAGTG 5 cut(s) 1230, 1836, 3928, 4330, 5759
BtuMI TCGCGA 2 cut(s) 5073, 6821
BveI ACCTGC 4 cut(s) 1422, 2302, 4466, 5551
CaiI CAGNNNCTG 3 cut(s) 5380, 5543, 6769
CciI TCATGA 3 cut(s) 1009, 3031, 6283
Cfr10I RCCGGY 2 cut(s) 5688, 6957
Cfr13I GGNCC 9 cut(s) 505, 2068, 2516, 2705, 3502, 4535, 6156, 6778, 7097
ClaI ATCGAT 2 cut(s) 1337, 6429
CseI GACGC 2 cut(s) 1046, 5336
CsiI ACCWGGT 2 cut(s) 2792, 4351
CspAI ACCGGT 1 cut(s) 5688
CspCI CAANNNNNGTGG 4 cut(s) 3789, 3824, 7089, 7124
DraI TTTAAA 1 cut(s) 6423
DraIII CACNNNGTG 2 cut(s) 2717, 2726
EaeI YGGCCR 2 cut(s) 1231, 2363
Eam1104I CTCTTC 4 cut(s) 2145, 3150, 3780, 4262
EarI CTCTTC 4 cut(s) 2145, 3150, 3780, 4262
Ecl136II GAGCTC 3 cut(s) 91, 604, 5101
Eco105I TACGTA 1 cut(s) 1713
Eco147I AGGCCT 1 cut(s) 2330
Eco24I GRGCYC 4 cut(s) 93, 606, 3232, 5103
Eco31I GGTCTC 2 cut(s) 848, 7129
Eco32I GATATC 2 cut(s) 1579, 2892
Eco47I GGWCC 6 cut(s) 2068, 2516, 2705, 3502, 6778, 7097
Eco53kI GAGCTC 3 cut(s) 91, 604, 5101
Eco57I CTGAAG 9 cut(s) 1194, 1389, 2031, 3446, 4091, 5687, 6587, 6665, 6810
Eco72I CACGTG 1 cut(s) 3808
Eco88I CYCGRG 3 cut(s) 845, 3231, 4331
Eco91I GGTNACC 1 cut(s) 4885
EcoICRI GAGCTC 3 cut(s) 91, 604, 5101
EcoNI CCTNNNNNAGG 2 cut(s) 2775, 3732
EcoO109I RGGNCCY 1 cut(s) 4535
EcoO65I GGTNACC 1 cut(s) 4885
EcoRI GAATTC 4 cut(s) 294, 454, 4099, 4429
EcoRV GATATC 2 cut(s) 1579, 2892
EcoT22I ATGCAT 7 cut(s) 319, 865, 1267, 2284, 4843, 5023, 6043
EcoT38I GRGCYC 4 cut(s) 93, 606, 3232, 5103
FaqI GGGAC 3 cut(s) 2502, 4321, 7110
FauI CCCGC 4 cut(s) 1515, 4273, 4627, 7085
FauNDI CATATG 1 cut(s) 3268
FbaI TGATCA 3 cut(s) 3718, 4129, 6633
FblI GTMKAC 3 cut(s) 1300, 4310, 5662
FriOI GRGCYC 4 cut(s) 93, 606, 3232, 5103
FspI TGCGCA 2 cut(s) 2288, 6702
GsaI CCCAGC 2 cut(s) 4093, 4654
GsuI CTGGAG 6 cut(s) 32, 476, 1634, 2850, 3338, 5478
HaeII RGCGCY 1 cut(s) 6799
HaeIII GGCC 9 cut(s) 507, 1211, 1233, 2330, 2365, 4536, 5163, 6157, 6961
HgaI GACGC 2 cut(s) 1046, 5336
HincII GTYRAC 4 cut(s) 1695, 5326, 5962, 6955
HindII GTYRAC 4 cut(s) 1695, 5326, 5962, 6955
HindIII AAGCTT 5 cut(s) 1238, 3197, 4871, 4878, 5084
HpaI GTTAAC 1 cut(s) 5962
HphI GGTGA 8 cut(s) 2501, 3672, 3756, 4649, 4770, 4940, 5293, 6112
HpyCH4IV ACGT 9 cut(s) 1712, 3807, 4289, 4490, 4795, 6074, 6169, 6460, 6943
HpySE526I ACGT 9 cut(s) 1712, 3807, 4289, 4490, 4795, 6074, 6169, 6460, 6943
Kpn2I TCCGGA 1 cut(s) 5362
KpnI GGTACC 1 cut(s) 5590
Ksp22I TGATCA 3 cut(s) 3718, 4129, 6633
KspAI GTTAAC 1 cut(s) 5962
LguI GCTCTTC 1 cut(s) 3150
MabI ACCWGGT 2 cut(s) 2792, 4351
MaeII ACGT 9 cut(s) 1712, 3807, 4289, 4490, 4795, 6074, 6169, 6460, 6943
MbiI CCGCTC 2 cut(s) 4634, 5134
MfeI CAATTG 6 cut(s) 1543, 2048, 2055, 3204, 3870, 4227
MflI RGATCY 8 cut(s) 420, 3128, 3241, 4174, 5357, 5539, 5989, 6403
MlsI TGGCCA 1 cut(s) 1233
MluNI TGGCCA 1 cut(s) 1233
MmeI TCCRAC 6 cut(s) 1825, 2623, 4609, 6115, 6190, 6918
Mox20I TGGCCA 1 cut(s) 1233
Mph1103I ATGCAT 7 cut(s) 319, 865, 1267, 2284, 4843, 5023, 6043
MroI TCCGGA 1 cut(s) 5362
MroXI GAANNNNTTC 3 cut(s) 201, 294, 4759
MscI TGGCCA 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 1233
MspA1I CMGCKG 5 cut(s) 496, 1207, 4093, 5380, 6608
MspCI CTTAAG 2 cut(s) 3223, 6062
MunI CAATTG 6 cut(s) 1543, 2048, 2055, 3204, 3870, 4227
Mva1269I GAATGC 4 cut(s) 865, 1040, 6220, 6348
MvnI CGCG 3 cut(s) 26, 5073, 6821
NciI CCSGG 4 cut(s) 972, 2575, 4539, 6293
NcoI CCATGG 4 cut(s) 1467, 2512, 3975, 6968
NdeI CATATG 1 cut(s) 3268
NmeAIII GCCGAG 1 cut(s) 4484
NmuCI GTSAC 2 cut(s) 3762, 5881
NruI TCGCGA 2 cut(s) 5073, 6821
NsbI TGCGCA 2 cut(s) 2288, 6702
NsiI ATGCAT 7 cut(s) 319, 865, 1267, 2284, 4843, 5023, 6043
NspI RCATGY 6 cut(s) 521, 1073, 1157, 1256, 4145, 4439
OliI CACNNNNGTG 1 cut(s) 1545
PaeR7I CTCGAG 1 cut(s) 845
PagI TCATGA 3 cut(s) 1009, 3031, 6283
PaqCI CACCTGC 1 cut(s) 5551
PceI AGGCCT 1 cut(s) 2330
PciSI GCTCTTC 1 cut(s) 3150
PcsI WCGNNNNNNNCGW 1 cut(s) 2773
PctI GAATGC 4 cut(s) 865, 1040, 6220, 6348
PdmI GAANNNNTTC 3 cut(s) 201, 294, 4759
PflMI CCANNNNNTGG 2 cut(s) 4352, 5278
PfoI TCCNGGA 3 cut(s) 1611, 6291, 6912
PinAI ACCGGT 1 cut(s) 5688
Ple19I CGATCG 2 cut(s) 1337, 6819
PmaCI CACGTG 1 cut(s) 3808
PmlI CACGTG 1 cut(s) 3808
Ppu21I YACGTR 3 cut(s) 1713, 3808, 6461
PshBI ATTAAT 4 cut(s) 285, 2904, 4623, 5433
PsiI TTATAA 3 cut(s) 1410, 2900, 3140
Psp124BI GAGCTC 3 cut(s) 93, 606, 5103
Psp1406I AACGTT 2 cut(s) 4289, 6169
PspCI CACGTG 1 cut(s) 3808
PspEI GGTNACC 1 cut(s) 4885
PspFI CCCAGC 2 cut(s) 4089, 4650
PspPI GGNCC 9 cut(s) 505, 2068, 2516, 2705, 3502, 4535, 6156, 6778, 7097
PsrI GAACNNNNNNTAC 2 cut(s) 4131, 4163
PstI CTGCAG 4 cut(s) 555, 2203, 2299, 4459
PstNI CAGNNNCTG 3 cut(s) 5380, 5543, 6769
PsuI RGATCY 8 cut(s) 420, 3128, 3241, 4174, 5357, 5539, 5989, 6403
PvuI CGATCG 2 cut(s) 1337, 6819
PvuII CAGCTG 4 cut(s) 1207, 4093, 5380, 6608
RgaI GCGATCGC 1 cut(s) 6819
RruI TCGCGA 2 cut(s) 5073, 6821
SacI GAGCTC 3 cut(s) 93, 606, 5103
SapI GCTCTTC 1 cut(s) 3150
Sau96I GGNCC 9 cut(s) 505, 2068, 2516, 2705, 3502, 4535, 6156, 6778, 7097
ScaI AGTACT 3 cut(s) 2410, 2972, 4453
SexAI ACCWGGT 2 cut(s) 2792, 4351
SfaAI GCGATCGC 1 cut(s) 6819
SfcI CTRYAG 8 cut(s) 137, 551, 1968, 2199, 2295, 3930, 4455, 5372
Sfr274I CTCGAG 1 cut(s) 845
SgfI GCGATCGC 1 cut(s) 6819
SinI GGWCC 6 cut(s) 2068, 2516, 2705, 3502, 6778, 7097
SlaI CTCGAG 1 cut(s) 845
SnaBI TACGTA 1 cut(s) 1713
SpeI ACTAGT 1 cut(s) 5105
SseBI AGGCCT 1 cut(s) 2330
SspI AATATT 8 cut(s) 934, 1572, 1825, 2815, 2911, 3796, 3999, 4120
SstI GAGCTC 3 cut(s) 93, 606, 5103
StuI AGGCCT 1 cut(s) 2330
TaiI ACGT 9 cut(s) 1715, 3810, 4292, 4493, 4798, 6077, 6172, 6463, 6946
TatI WGTACW 8 cut(s) 1291, 2408, 2970, 4147, 4219, 4451, 5196, 6876
TauI GCSGC 4 cut(s) 2368, 4402, 4771, 6749
TseFI GTSAC 2 cut(s) 3762, 5881
Tsp45I GTSAC 2 cut(s) 3762, 5881
TspGWI ACGGA 4 cut(s) 67, 596, 5543, 5687
Van91I CCANNNNNTGG 2 cut(s) 4352, 5278
Vha464I CTTAAG 2 cut(s) 3223, 6062
VneI GTGCAC 1 cut(s) 2718
VpaK11BI GGWCC 6 cut(s) 2068, 2516, 2705, 3502, 6778, 7097
VspI ATTAAT 4 cut(s) 285, 2904, 4623, 5433
XagI CCTNNNNNAGG 2 cut(s) 2775, 3732
XbaI TCTAGA 1 cut(s) 5971
XceI RCATGY 6 cut(s) 521, 1073, 1157, 1256, 4145, 4439
XcmI CCANNNNNNNNNTGG 2 cut(s) 1103, 3357
XhoI CTCGAG 1 cut(s) 845
XmiI GTMKAC 3 cut(s) 1300, 4310, 5662
XmnI GAANNNNTTC 3 cut(s) 201, 294, 4759
ZrmI AGTACT 3 cut(s) 2410, 2972, 4453
Zsp2I ATGCAT 7 cut(s) 319, 865, 1267, 2284, 4843, 5023, 6043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.