Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008431

Physical Location & Seq

Genomic Coordinates Reverse (-)

1369036 .. 1373103

Gene Structure 4068 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G04860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2886 bp

ATGGGGAAGAAAGCGAAGAAGAAGGCCCGAGCTCCAACAAAGGAGATTCAGACCATGGAAATTTCCAAGAAGGTCTCAGAAGAGCCGCCTAGTCAAGCCGGGGAAATTGCTGAGGGTGACGTTAAAGCAGTTAAGGAGACACAAGCTTGTGTGCATTTCGATAAGGCTCTTAATTTGGAGAAGGTGTTGGATAAGATTAAGTCATCCCGGCAGATTAAGTGTGCAGAATGCAATGAAGGAGTGTATGGAAAGAGAGGAACTAAAGCAAAGGGCAGTAAAGGGAAGAAGGATTTTTCTTCTTCTGATCCAAAATCTAATAACAAAGCCATTTGGCTATGTTTGGAATGTGGCTGTTATGTTTGTGGAGGTGTTGGTTTACCAAATGGACCTCAGAGCCATGTTTTGCGACACAGCAGAGTGACACGTCACCGATTGGTGATTCAATGGGAAAATCCTCAGTTAAGATGGTGTTTCCCGTGCCAGTTGCTTCTCCCTGTTGAAAAAGAAGATAATGGCGAGAAGAAAGATGTACTGTCAGAGGTTGTTAAATTGATTAAAGGACGATCTTTGAACAATTTAGCTTCATCCGATATCGAAGATCAGTGCTCAGGAAGTGGCAGTATCACTAGTGATATCAAACTAGAAGGTGCTGTGACCAGTGATATAGAAGCAAGAGATGGTTATGTTGTGCGAGGTTTAGTTAACCTTGGGAACACATGTTTCTTTAATTCGATAATGCAGAATCTTCTTTCTTTGGATCGGTTACGCGATCACTTCTTGAAAGAGAATGGGTCTGGGGTCGGTGGGCCTCTTGCGTCTTCCCTGAGGAAATTATTCACTGAAACGAAACCAGAGGCAGGCTTGAAGAGTGTGATAAATCCTAGAGCCTTTTTTGGGTCTTTCTGTTCTAAGGCACCCCAGTTCCGGGGATATGACCAGCATGATAGCCACGAGTTGCTACGCTGTTTGCTAGATTCGTTGAGCACCGAAGAATCGGCCTTGAGAAAGAAGCGTGGTGTTTCTGATAATGATGAGAAATCTACTACTCTCATAGAATCTGTATTTGGAGGCGAAACGTCGAGCATTGTTAGTTGTATGGAGTGCGGGCATTCCTCAAAAGTCTATGAACCATTTTTGGATCTCTCCTTACCCGTACCATTCAAGAAAAGCCCTCCTAAAAAACCACAACCTGTTTCTCGAGCAAAAAAAGCTAAGCTTCCACCTAAGAGAGTCCCAAAGAATGTGTCAAAAGTAAGTAAGGTTTCAAAAGTTCTCCCAGGTATGGTTCTTTCGGAACTGAACTCTTCCGGAAAATCTATGGCTGTCACAGCTGATTCGGATACATCCTGTTCCAGTTTAGCACCCCTTGATAATGGTCCGGTTTTAGAAACTCCTTCAGTTCTAACCCTTGACAATAATCAGGCCTCAGAAAGTGCATCACAAAGTGATACTGGTTTCGATGGCTCCTGGTTGGATTTTATCGGACCAGAAACCTCTGGAGATGAGACGAATTTGGATATGCAAGAAGATGGTATTGATAATGTCATTACAGCTGAGGTTAATCAAATTGTTCCCAGTCCAAATATTGTGGCGAATAGCTCAGTTTCATCGGGTGATCAGACTTTGGAAGGTAATACAGAGAGGCTGATGCAAGATTATGAAGAAATTGCAAAGGCGGAAGCCAATTTGGATGAAAAAGATGTACAAGCTATGCAGTCTGATGAATGCCCAGCTACAAGTGGTATTTCTGCTGAATTCAGTCAAGCTAGTTGCATTGGTTGTGATCCTGGTATAGGGGAAAGTTCTTCCTCGGTGAATCCTTGGGACGAGGAAGAGCTTCCTTTGGTGGTTGCAGATTCGCAAATTTTGTACATGCCATACAAAGAAATATCCTGTAATGATAAATCAGTTGAAGGTGAGTGTGAAGCTTCATCTTCATTTGTTACTGGTGATCATGAACCACAAAACAGTGATTTTGTTGATTTTGGCGGTTTATTTGATGAGCCTGAGACTACTGAGGGACCTGTTTTTGGACCTCCTTCCAAGGCTGAAGCCTCGGGAGTTGGTTTTATGGCGTTCAGCAGCGAGTCTGATCCTGAAGAAATCGATGATTCGGATTTACCGGTGTCTGTAGAGAGGTGTTTGGGTCATTTCACAAAACATGAGATACTGTCAGATGACAATGCTTGGAACTGCGAGAACTGTTCAAAGAACCTGAAACTCCAACGGTTGAGAGAAAAGCGAAAATCCAATGAAGATGAATCGAGATCAAGCAACACCAGCAACGGATGGGTGAAAGAAAATGAAGATGAAGGTTTTGGAGAAACCGAAATTTTGGCTGTTAAACAAGATCCAAATGATACTAGTTGTGTCAAGGATCATAGCTCTGATGGAAGAAAAGCTGCGAGGATTCACTCAGCTGATGAAAGTGAATCCAAAGGAACTCAAGATGAAGACGAAGATTCTGAGAAGGTGATTACAGTGAAGAGAGATGCAACTAAAAAAGTACTTATAAACAAAGCCCCACCTGTCCTAACCATTCATCTAAAACGATTCAGCCAAGATTTGCGTGGTAGGCTAAGTAAATTAAATGGTCATGTTGCTTTCAAAGAGGTCATCGATCTTCGACAATATATGGACTCAAGGTGTAGCGGAGAAGACCCGCCCGTTTACAGACTGGCTGGATTGGTGGAGCATTCAGGGACGATGAGAGGAGGTCATTATGTGGCGTATGTTAGAGGAGGTCAGCGGGTTAAAGAGACTGATTCCTCCTCCACTGCGTGGTATAACGTAAGCGATGCATATGTTCGTCAGGTTTCATTGGAAAAGGTTCTGCATTCTGAAGCGTATATCTTGTTCTATGAACGGATCTTCTCACAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

961

Amino Acids

105.15

Weight (kDa)

5.15

Isoelectric Point (pI)

48.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	74 - 161	1.5e-10	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	231 - 954	2.2e-55	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04860
fragaria_vesca	FvH4_4g16490 FvH4_4g16490
malus_domestica	MD04G1009400.v1.1 MD13G1284700.v1.1
prunus_persica	Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1
pyrus_communis	pycom04g00720 pycom420g01370
rosa_chinensis	RchiOBHm_Chr4g0415171
rosa_laevigata	RLG00000008090
rosa_multiflora	Rmu_sc0003748.1_g000031 Rmu_sc0033472.1_g000001
rosa_roxburghii	Rroxscaffold_5G00359000
rosa_rugosa	Rorug04G0132500 Rorug04G0132500
rosa_samantha	Rh4AG192800 Rh4BG190900 Rh4CG203600 Rh4DG190700
rosa_wichuraiana	Rw4G016390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2513
AccB1I	GGYRCC	1 cut(s)	913
AccB7I	CCANNNNNTGG	1 cut(s)	2781
AccII	CGCG	1 cut(s)	768
AccIII	TCCGGA	1 cut(s)	1307
AciI	CCGC	7 cut(s)	86, 1104, 1676, 1989, 2652, 2663, 2749
AclWI	GGATC	8 cut(s)	299, 765, 1146, 1778, 2087, 2345, 2385, 2876
AcsI	RAATTY	5 cut(s)	60, 1510, 1754, 1863, 2331
AcuI	CTGAAG	4 cut(s)	1380, 2070, 2118, 2862
AdeI	CACNNNGTG	2 cut(s)	1445, 2781
AfaI	GTAC	5 cut(s)	531, 1155, 1704, 1871, 2508
AfiI	CCNNNNNNNGG	8 cut(s)	857, 894, 924, 925, 1282, 1793, 2030, 2781
AflIII	ACRYGT	2 cut(s)	422, 716
AgeI	ACCGGT	1 cut(s)	2122
AhlI	ACTAGT	2 cut(s)	626, 2363
AjiI	CACGTC	1 cut(s)	425
AjnI	CCWGG	3 cut(s)	1276, 1466, 1786
AjuI	GAANNNNNNNTTGG	2 cut(s)	1047, 1079
Alw21I	GWGCWC	3 cut(s)	34, 608, 986
Alw26I	GTCTC	5 cut(s)	79, 131, 1499, 2003, 2753
AlwI	GGATC	8 cut(s)	299, 765, 1146, 1778, 2087, 2345, 2385, 2876
Ama87I	CYCGRG	3 cut(s)	27, 1197, 2056
Aor13HI	TCCGGA	1 cut(s)	1307
AoxI	GGCC	4 cut(s)	24, 806, 996, 1422
ApeKI	GCWGC	2 cut(s)	2082, 2402
ApoI	RAATTY	5 cut(s)	60, 1510, 1754, 1863, 2331
ArsI	GACNNNNNNTTYG	2 cut(s)	1229, 1261
AsiGI	ACCGGT	1 cut(s)	2122
Asp700I	GAANNNNTTC	3 cut(s)	833, 1836, 2829
AspS9I	GGNCC	7 cut(s)	25, 386, 806, 1376, 1484, 2021, 2033
AsuC2I	CCSGG	3 cut(s)	100, 208, 926
AsuHPI	GGTGA	9 cut(s)	128, 419, 448, 1625, 1825, 1928, 1961, 2305, 2485
AvaI	CYCGRG	3 cut(s)	27, 1197, 2056
AvaII	GGWCC	5 cut(s)	386, 1376, 1484, 2021, 2033
AxyI	CCTNAGG	1 cut(s)	824
BanI	GGYRCC	1 cut(s)	913
BanII	GRGCYC	1 cut(s)	34
BauI	CACGAG	1 cut(s)	950
BbsI	GAAGAC	3 cut(s)	810, 2460, 2664
Bbv12I	GWGCWC	3 cut(s)	34, 608, 986
BbvCI	CCTCAGC	2 cut(s)	111, 1554
BbvI	GCAGC	2 cut(s)	2094, 2389
BccI	CCATC	6 cut(s)	459, 671, 1454, 1523, 2283, 2384
BciT130I	CCWGG	3 cut(s)	1278, 1468, 1788
BciVI	GTATCC	1 cut(s)	1333
BclI	TGATCA	2 cut(s)	1615, 1951
BcnI	CCSGG	3 cut(s)	100, 208, 926
BcoDI	GTCTC	5 cut(s)	79, 131, 1499, 2003, 2753
BcuI	ACTAGT	2 cut(s)	626, 2363
BfaI	CTAG	7 cut(s)	90, 627, 641, 882, 971, 1767, 2364
BfmI	CTRYAG	1 cut(s)	2130
BfuI	GTATCC	1 cut(s)	1333
BisI	GCNGC	3 cut(s)	86, 2083, 2403
BlpI	GCTNAGC	1 cut(s)	1212
BlsI	GCNGC	3 cut(s)	87, 2084, 2404
BmcAI	AGTACT	1 cut(s)	2508
Bme1390I	CCNGG	6 cut(s)	100, 208, 926, 1278, 1468, 1788
Bme18I	GGWCC	5 cut(s)	386, 1376, 1484, 2021, 2033
BmeT110I	CYCGRG	3 cut(s)	27, 1197, 2056
BmgBI	CACGTC	1 cut(s)	425
BmgT120I	GGNCC	7 cut(s)	25, 386, 806, 1376, 1484, 2021, 2033
BmiI	GGNNCC	3 cut(s)	915, 1465, 2022
BmrFI	CCNGG	6 cut(s)	100, 208, 926, 1278, 1468, 1788
BmrI	ACTGGG	2 cut(s)	913, 1569
BmsI	GCATC	4 cut(s)	1445, 1638, 2482, 2788
BmuI	ACTGGG	2 cut(s)	913, 1569
BpiI	GAAGAC	3 cut(s)	810, 2460, 2664
BpmI	CTGGAG	1 cut(s)	1518
Bpu10I	CCTNAGC	3 cut(s)	111, 607, 1554
Bpu1102I	GCTNAGC	1 cut(s)	1212
BpuEI	CTTGAG	3 cut(s)	1021, 2430, 2626
BpuMI	CCSGG	3 cut(s)	100, 208, 926
Bsa29I	ATCGAT	2 cut(s)	2106, 2619
BsaI	GGTCTC	1 cut(s)	79
BsaJI	CCNNGG	9 cut(s)	54, 99, 706, 925, 1276, 1809, 1820, 2043, 2055
BsaWI	WCCGGW	3 cut(s)	1307, 1378, 2122
BsaXI	ACNNNNNCTCC	2 cut(s)	170, 200
Bsc4I	CCNNNNNNNGG	8 cut(s)	857, 894, 924, 925, 1282, 1793, 2030, 2781
Bse118I	RCCGGY	1 cut(s)	2122
Bse1I	ACTGG	8 cut(s)	481, 657, 919, 1353, 1456, 1575, 1951, 2682
Bse21I	CCTNAGG	1 cut(s)	824
Bse3DI	GCAATG	1 cut(s)	238
BseAI	TCCGGA	1 cut(s)	1307
BseBI	CCWGG	3 cut(s)	1278, 1468, 1788
BseCI	ATCGAT	2 cut(s)	2106, 2619
BseDI	CCNNGG	9 cut(s)	54, 99, 706, 925, 1276, 1809, 1820, 2043, 2055
BseGI	GGATG	5 cut(s)	203, 584, 1343, 1696, 2294
BseLI	CCNNNNNNNGG	8 cut(s)	857, 894, 924, 925, 1282, 1793, 2030, 2781
BseMI	GCAATG	1 cut(s)	238
BseNI	ACTGG	8 cut(s)	481, 657, 919, 1353, 1456, 1575, 1951, 2682
BseRI	GAGGAG	3 cut(s)	2727, 2754, 2761
BseXI	GCAGC	2 cut(s)	2094, 2389
BseYI	CCCAGC	1 cut(s)	1729
BsgI	GTGCAG	1 cut(s)	243
Bsh1236I	CGCG	1 cut(s)	768
BshFI	GGCC	4 cut(s)	26, 808, 998, 1424
BshNI	GGYRCC	1 cut(s)	913
BshTI	ACCGGT	1 cut(s)	2122
BshVI	ATCGAT	2 cut(s)	2106, 2619
BsiHKAI	GWGCWC	3 cut(s)	34, 608, 986
BsiHKCI	CYCGRG	3 cut(s)	27, 1197, 2056
BsiSI	CCGG	6 cut(s)	99, 208, 925, 1308, 1379, 2123
BslFI	GGGAC	4 cut(s)	1217, 1838, 2034, 2716
BslI	CCNNNNNNNGG	8 cut(s)	857, 894, 924, 925, 1282, 1793, 2030, 2781
BsmAI	GTCTC	5 cut(s)	79, 131, 1499, 2003, 2753
BsmBI	CGTCTC	1 cut(s)	1499
BsmFI	GGGAC	4 cut(s)	1217, 1838, 2034, 2716
BsmI	GAATGC	5 cut(s)	233, 1108, 1730, 2695, 2836
BsnI	GGCC	4 cut(s)	26, 808, 998, 1424
Bso31I	GGTCTC	1 cut(s)	79
BsoBI	CYCGRG	3 cut(s)	27, 1197, 2056
Bsp1286I	GDGCHC	3 cut(s)	34, 608, 986
Bsp13I	TCCGGA	1 cut(s)	1307
Bsp1407I	TGTACA	2 cut(s)	1702, 1869
Bsp1720I	GCTNAGC	1 cut(s)	1212
Bsp19I	CCATGG	1 cut(s)	54
BspACI	CCGC	7 cut(s)	86, 1104, 1676, 1989, 2652, 2663, 2749
BspANI	GGCC	4 cut(s)	26, 808, 998, 1424
BspDI	ATCGAT	2 cut(s)	2106, 2619
BspEI	TCCGGA	1 cut(s)	1307
BspFNI	CGCG	1 cut(s)	768
BspHI	TCATGA	1 cut(s)	1954
BspLI	GGNNCC	3 cut(s)	915, 1465, 2022
BspPI	GGATC	8 cut(s)	299, 765, 1146, 1778, 2087, 2345, 2385, 2876
BspQI	GCTCTTC	2 cut(s)	75, 1827
BspT107I	GGYRCC	1 cut(s)	913
BspTNI	GGTCTC	1 cut(s)	79
BsrDI	GCAATG	1 cut(s)	238
BsrFI	RCCGGY	1 cut(s)	2122
BsrGI	TGTACA	2 cut(s)	1702, 1869
BsrI	ACTGG	8 cut(s)	481, 657, 919, 1353, 1456, 1575, 1951, 2682
BssAI	RCCGGY	1 cut(s)	2122
BssECI	CCNNGG	9 cut(s)	54, 99, 706, 925, 1276, 1809, 1820, 2043, 2055
BssSI	CACGAG	1 cut(s)	950
BssT1I	CCWWGG	4 cut(s)	54, 706, 1820, 2043
Bst2BI	CACGAG	1 cut(s)	950
Bst2UI	CCWGG	3 cut(s)	1278, 1468, 1788
Bst4CI	ACNGT	6 cut(s)	534, 1970, 2172, 2204, 2229, 2482
Bst6I	CTCTTC	5 cut(s)	75, 860, 1309, 1827, 2480
BstAUI	TGTACA	2 cut(s)	1702, 1869
BstC8I	GCNNGC	2 cut(s)	859, 1106
BstDSI	CCRYGG	1 cut(s)	54
BstENI	CCTNNNNNAGG	1 cut(s)	1791
BstF5I	GGATG	5 cut(s)	203, 584, 1343, 1696, 2294
BstFNI	CGCG	1 cut(s)	768
BstMAI	GTCTC	5 cut(s)	79, 131, 1499, 2003, 2753
BstMWI	GCNNNNNNNGC	4 cut(s)	1208, 1328, 2280, 2575
BstNI	CCWGG	3 cut(s)	1278, 1468, 1788
BstNSI	RCATGY	2 cut(s)	720, 1876
BstSCI	CCNGG	6 cut(s)	98, 206, 924, 1276, 1466, 1786
BstSFI	CTRYAG	1 cut(s)	2130
BstUI	CGCG	1 cut(s)	768
BstV1I	GCAGC	2 cut(s)	2094, 2389
BstV2I	GAAGAC	3 cut(s)	810, 2460, 2664
BstX2I	RGATCY	3 cut(s)	1138, 2350, 2868
BstYI	RGATCY	3 cut(s)	1138, 2350, 2868
Bsu15I	ATCGAT	2 cut(s)	2106, 2619
Bsu36I	CCTNAGG	1 cut(s)	824
BsuI	GTATCC	1 cut(s)	1333
BsuRI	GGCC	4 cut(s)	26, 808, 998, 1424
BsuTUI	ATCGAT	2 cut(s)	2106, 2619
BtgI	CCRYGG	1 cut(s)	54
BtgZI	GCGATG	1 cut(s)	2811
BtrI	CACGTC	1 cut(s)	425
BtsCI	GGATG	5 cut(s)	203, 584, 1343, 1696, 2294
BtsI	GCAGTG	1 cut(s)	2775
BtsIMutI	CAGTG	6 cut(s)	608, 664, 837, 1975, 2487, 2775
Cac8I	GCNNGC	2 cut(s)	859, 1106
CciI	TCATGA	1 cut(s)	1954
Cfr10I	RCCGGY	1 cut(s)	2122
Cfr13I	GGNCC	7 cut(s)	25, 386, 806, 1376, 1484, 2021, 2033
ClaI	ATCGAT	2 cut(s)	2106, 2619
CseI	GACGC	1 cut(s)	804
Csp6I	GTAC	5 cut(s)	530, 1154, 1703, 1870, 2507
CspAI	ACCGGT	1 cut(s)	2122
CspCI	CAANNNNNGTGG	2 cut(s)	1569, 1604
CviAII	CATG	8 cut(s)	55, 398, 717, 941, 1873, 1955, 2162, 2597
CviQI	GTAC	5 cut(s)	530, 1154, 1703, 1870, 2507
DraIII	CACNNNGTG	2 cut(s)	1445, 2781
Eam1104I	CTCTTC	5 cut(s)	75, 860, 1309, 1827, 2480
EarI	CTCTTC	5 cut(s)	75, 860, 1309, 1827, 2480
EciI	GGCGGA	1 cut(s)	1691
Ecl136II	GAGCTC	1 cut(s)	32
Eco130I	CCWWGG	4 cut(s)	54, 706, 1820, 2043
Eco147I	AGGCCT	1 cut(s)	1424
Eco24I	GRGCYC	1 cut(s)	34
Eco31I	GGTCTC	1 cut(s)	79
Eco32I	GATATC	2 cut(s)	592, 634
Eco47I	GGWCC	5 cut(s)	386, 1376, 1484, 2021, 2033
Eco53kI	GAGCTC	1 cut(s)	32
Eco57I	CTGAAG	4 cut(s)	1380, 2070, 2118, 2862
Eco81I	CCTNAGG	1 cut(s)	824
Eco88I	CYCGRG	3 cut(s)	27, 1197, 2056
EcoICRI	GAGCTC	1 cut(s)	32
EcoNI	CCTNNNNNAGG	1 cut(s)	1791
EcoO109I	RGGNCCY	1 cut(s)	2021
EcoRI	GAATTC	1 cut(s)	1754
EcoRII	CCWGG	3 cut(s)	1276, 1466, 1786
EcoRV	GATATC	2 cut(s)	592, 634
EcoT14I	CCWWGG	4 cut(s)	54, 706, 1820, 2043
EcoT22I	ATGCAT	1 cut(s)	2803
EcoT38I	GRGCYC	1 cut(s)	34
ErhI	CCWWGG	4 cut(s)	54, 706, 1820, 2043
Esp3I	CGTCTC	1 cut(s)	1499
FaeI	CATG	8 cut(s)	58, 401, 720, 944, 1876, 1958, 2165, 2600
FalI	AAGNNNNNCTT	2 cut(s)	1200, 1232
FaqI	GGGAC	4 cut(s)	1217, 1838, 2034, 2716
FatI	CATG	8 cut(s)	54, 397, 716, 940, 1872, 1954, 2161, 2596
FauI	CCCGC	3 cut(s)	1097, 2670, 2742
FauNDI	CATATG	1 cut(s)	2803
FbaI	TGATCA	2 cut(s)	1615, 1951
Fnu4HI	GCNGC	3 cut(s)	86, 2083, 2403
FokI	GGATG	5 cut(s)	190, 571, 1330, 1703, 2301
FriOI	GRGCYC	1 cut(s)	34
Fsp4HI	GCNGC	3 cut(s)	86, 2083, 2403
FspBI	CTAG	7 cut(s)	90, 627, 641, 882, 971, 1767, 2364
GluI	GCNGC	3 cut(s)	86, 2083, 2403
GsaI	CCCAGC	1 cut(s)	1733
GsuI	CTGGAG	1 cut(s)	1518
HaeIII	GGCC	4 cut(s)	26, 808, 998, 1424
HapII	CCGG	6 cut(s)	99, 208, 925, 1308, 1379, 2123
HgaI	GACGC	1 cut(s)	804
Hin1II	CATG	8 cut(s)	58, 401, 720, 944, 1876, 1958, 2165, 2600
HincII	GTYRAC	1 cut(s)	703
HindII	GTYRAC	1 cut(s)	703
HindIII	AAGCTT	3 cut(s)	144, 1214, 1926
HpaI	GTTAAC	1 cut(s)	703
HpaII	CCGG	6 cut(s)	99, 208, 925, 1308, 1379, 2123
HphI	GGTGA	9 cut(s)	128, 419, 448, 1625, 1825, 1928, 1961, 2305, 2485
Hpy166II	GTNNAC	3 cut(s)	377, 703, 2671
Hpy8I	GTNNAC	3 cut(s)	377, 703, 2671
Hpy99I	CGWCG	1 cut(s)	1081
HpyCH4III	ACNGT	6 cut(s)	534, 1970, 2172, 2204, 2229, 2482
HpyCH4IV	ACGT	4 cut(s)	120, 424, 1076, 2790
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1208, 1328, 2280, 2575
HpySE526I	ACGT	4 cut(s)	120, 424, 1076, 2790
Hsp92II	CATG	8 cut(s)	58, 401, 720, 944, 1876, 1958, 2165, 2600
Kpn2I	TCCGGA	1 cut(s)	1307
Ksp22I	TGATCA	2 cut(s)	1615, 1951
KspAI	GTTAAC	1 cut(s)	703
LguI	GCTCTTC	2 cut(s)	75, 1827
LmnI	GCTCC	3 cut(s)	37, 1469, 2692
Lsp1109I	GCAGC	2 cut(s)	2094, 2389
LweI	GCATC	4 cut(s)	1445, 1638, 2482, 2788
MaeI	CTAG	7 cut(s)	90, 627, 641, 882, 971, 1767, 2364
MaeII	ACGT	4 cut(s)	120, 424, 1076, 2790
MaeIII	GTNAC	7 cut(s)	116, 418, 425, 652, 762, 1324, 1942
MflI	RGATCY	3 cut(s)	1138, 2350, 2868
MhlI	GDGCHC	3 cut(s)	34, 608, 986
MlyI	GAGTC	3 cut(s)	1239, 2096, 2633
MmeI	TCCRAC	4 cut(s)	59, 168, 1452, 2248
Mph1103I	ATGCAT	1 cut(s)	2803
MroI	TCCGGA	1 cut(s)	1307
MroXI	GAANNNNTTC	3 cut(s)	833, 1836, 2829
MslI	CAYNNNNRTG	1 cut(s)	2881
MspA1I	CMGCKG	4 cut(s)	1331, 1553, 2420, 2749
MspI	CCGG	6 cut(s)	99, 208, 925, 1308, 1379, 2123
MspR9I	CCNGG	6 cut(s)	100, 208, 926, 1278, 1468, 1788
Mva1269I	GAATGC	5 cut(s)	233, 1108, 1730, 2695, 2836
MvaI	CCWGG	3 cut(s)	1278, 1468, 1788
MvnI	CGCG	1 cut(s)	768
MwoI	GCNNNNNNNGC	4 cut(s)	1208, 1328, 2280, 2575
NciI	CCSGG	3 cut(s)	100, 208, 926
NcoI	CCATGG	1 cut(s)	54
NdeI	CATATG	1 cut(s)	2803
NlaIII	CATG	8 cut(s)	58, 401, 720, 944, 1876, 1958, 2165, 2600
NlaIV	GGNNCC	3 cut(s)	915, 1465, 2022
NmuCI	GTSAC	5 cut(s)	116, 418, 425, 652, 1324
NsiI	ATGCAT	1 cut(s)	2803
NspI	RCATGY	2 cut(s)	720, 1876
PaeR7I	CTCGAG	1 cut(s)	1197
PagI	TCATGA	1 cut(s)	1954
PceI	AGGCCT	1 cut(s)	1424
PciI	ACATGT	1 cut(s)	716
PciSI	GCTCTTC	2 cut(s)	75, 1827
PctI	GAATGC	5 cut(s)	233, 1108, 1730, 2695, 2836
PdmI	GAANNNNTTC	3 cut(s)	833, 1836, 2829
PflMI	CCANNNNNTGG	1 cut(s)	2781
PinAI	ACCGGT	1 cut(s)	2122
PkrI	GCNGC	3 cut(s)	87, 2084, 2404
PleI	GAGTC	3 cut(s)	1238, 2095, 2633
PpsI	GAGTC	3 cut(s)	1238, 2095, 2633
PpuMI	RGGWCCY	1 cut(s)	2021
PscI	ACATGT	1 cut(s)	716
PsiI	TTATAA	1 cut(s)	2513
Psp124BI	GAGCTC	1 cut(s)	34
Psp5II	RGGWCCY	1 cut(s)	2021
Psp6I	CCWGG	3 cut(s)	1276, 1466, 1786
PspFI	CCCAGC	1 cut(s)	1729
PspGI	CCWGG	3 cut(s)	1276, 1466, 1786
PspN4I	GGNNCC	3 cut(s)	915, 1465, 2022
PspPI	GGNCC	7 cut(s)	25, 386, 806, 1376, 1484, 2021, 2033
PspPPI	RGGWCCY	1 cut(s)	2021
PsrI	GAACNNNNNNTAC	2 cut(s)	2840, 2872
PsuI	RGATCY	3 cut(s)	1138, 2350, 2868
PvuII	CAGCTG	3 cut(s)	1331, 1553, 2420
RsaI	GTAC	5 cut(s)	531, 1155, 1704, 1871, 2508
RsaNI	GTAC	5 cut(s)	530, 1154, 1703, 1870, 2507
RseI	CAYNNNNRTG	1 cut(s)	2881
SacI	GAGCTC	1 cut(s)	34
SapI	GCTCTTC	2 cut(s)	75, 1827
SatI	GCNGC	3 cut(s)	86, 2083, 2403
Sau96I	GGNCC	7 cut(s)	25, 386, 806, 1376, 1484, 2021, 2033
ScaI	AGTACT	1 cut(s)	2508
SchI	GAGTC	3 cut(s)	1239, 2096, 2633
ScrFI	CCNGG	6 cut(s)	100, 208, 926, 1278, 1468, 1788
SduI	GDGCHC	3 cut(s)	34, 608, 986
SfaNI	GCATC	4 cut(s)	1445, 1638, 2482, 2788
SfcI	CTRYAG	1 cut(s)	2130
Sfr274I	CTCGAG	1 cut(s)	1197
SinI	GGWCC	5 cut(s)	386, 1376, 1484, 2021, 2033
SlaI	CTCGAG	1 cut(s)	1197
SmiMI	CAYNNNNRTG	1 cut(s)	2881
SmlI	CTYRAG	4 cut(s)	1000, 1197, 2445, 2641
SmoI	CTYRAG	4 cut(s)	1000, 1197, 2445, 2641
SpeI	ACTAGT	2 cut(s)	626, 2363
SseBI	AGGCCT	1 cut(s)	1424
SsiI	CCGC	7 cut(s)	86, 1104, 1676, 1989, 2652, 2663, 2749
SspI	AATATT	1 cut(s)	1585
SspMI	CTAG	7 cut(s)	90, 627, 641, 882, 971, 1767, 2364
SstI	GAGCTC	1 cut(s)	34
StuI	AGGCCT	1 cut(s)	1424
StyD4I	CCNGG	6 cut(s)	98, 206, 924, 1276, 1466, 1786
StyI	CCWWGG	4 cut(s)	54, 706, 1820, 2043
TaaI	ACNGT	6 cut(s)	534, 1970, 2172, 2204, 2229, 2482
TaiI	ACGT	4 cut(s)	123, 427, 1079, 2793
TatI	WGTACW	4 cut(s)	529, 1702, 1869, 2506
TauI	GCSGC	1 cut(s)	88
TscAI	CASTG	6 cut(s)	608, 664, 844, 1975, 2487, 2782
TseFI	GTSAC	5 cut(s)	116, 418, 425, 652, 1324
TseI	GCWGC	2 cut(s)	2082, 2402
Tsp45I	GTSAC	5 cut(s)	116, 418, 425, 652, 1324
TspGWI	ACGGA	2 cut(s)	2301, 2881
TspRI	CASTG	6 cut(s)	608, 664, 844, 1975, 2487, 2782
Van91I	CCANNNNNTGG	1 cut(s)	2781
VpaK11BI	GGWCC	5 cut(s)	386, 1376, 1484, 2021, 2033
XagI	CCTNNNNNAGG	1 cut(s)	1791
XapI	RAATTY	5 cut(s)	60, 1510, 1754, 1863, 2331
XceI	RCATGY	2 cut(s)	720, 1876
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2567
XhoI	CTCGAG	1 cut(s)	1197
XmnI	GAANNNNTTC	3 cut(s)	833, 1836, 2829
XspI	CTAG	7 cut(s)	90, 627, 641, 882, 971, 1767, 2364
ZrmI	AGTACT	1 cut(s)	2508
Zsp2I	ATGCAT	1 cut(s)	2803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.