Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0008431

Physical Location & Seq

Genomic Coordinates Reverse (-)

37472713 .. 37479325

Gene Structure 6613 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG190700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2868 bp

ATGGGGAAGAAGGTTAAGAAGAAGGCTAGAGCTCCTCAAAAGGAGAAGTGGGTTCCATCTGATTCCCCGAAGAAGGTTGCTGAACAAAGCAACCCAAGCGTTAAGGATGATGGTGATGATGGAGTTTCAGTTGCAAAAGTGAGAAAAGCTTGTCCTCACATTGACAAAGGTGTTGATTTGAATAAATTGCAAGCTAAAATTGGGTCTTCAGTAGTTGTTAGGTGTGAAGATTGTAGGGAAGGTGCCATTGATAGGAAGGGGGGTAAAGGAAAGGGGAAACATGGTAAGAAGAAAGGAGGTGCTGATTCTAAATCCGAGTCAAAATCTGTATGGCTTTGTTTAGAATGCGGTCATTTTGCTTGTGGTGGGGTTGGACTTCCAATAACCCCTCAGTGCCACGCAGTTCGGCATGCAAGGCAAACTCGTCACCCATTGGTGGTGCAGTTTGAAAACCCACAGTTGCGATGGTGCTTCCAGTGCAGCACACTTATTACCATTGATAAGACGGGGGAGAATGGTGAAGAAAAAGATGTCTTTTCTGATGTTGTGAAATTAATTAAGGGTCATTCGTCGGAGGACTCATCAGGTGATGTCGAGGATGTATGGTTTGGTAGTGGCAGTGTGACAAGCGAAATCAAATCAGCTAGCAATACATCAAGCCTTTTGGATGGAAGAGGAGGTTATGCAGTAAGAGGCTTAGTTAATCTTGGTAACACTTGCTTCTTCAATTCTATTTTACAGAACCTTCTAGCTATTGATAGGTTGCGGGTTTACTTCTTAAATTTTGAAGCACCTGCCGGACCGCTTACTATTTCCTTGAAGAAACTCTTTACTGAAACAAAACCAGAGGCAGGATTCAAGAATGTGATAAACCCTAGATCGTTTTTCGGTTGCGTCTGTTCCAAGGCTCCCCAGTTTAGGGGCTATCAGCAGCAAGACAGTCATGAATTGCTTCGTTGCTTACTGGATGGATTGTGTACTGAGGAGTTGAGTATGAGGAAACGTACAAGTTCTTCCCAGCAAAATGAGGATTCATCAAATCCAGTTCCTACGTTTGTTGATGCTGTATTTGGGGGACAGGTATCAAGTACTGTTTGCTGTGTTGAATGTGGTCACTCATCAACTATCTATGAGTCATTCTTAGATCTGTCACTGCCAGTCCCAACCAGGAAATCCCCACCTAAGGCTTCCCTACCAAGCTCTCGAGCAAAGAAGACGAAGCTTCCACCCAAGAGGAGTGGAAAGGTTCGATCTAAAATTAACAAAGACAAGAACTCTGCAACATCTCCAAGTGTGGCAACCACATCAACCAGCGGTGAGCTCTCTAACCAGGTACAGTCAAGTTCTACTGAGCTAAATGTACCTGAACAAAACGGCTTGGTTATGAAGAATCTATCAGCTGTCCAAGAATCTCAACATGAACAAGTCTTTGAGAATGCAGCAGGAGAACAAGCATCAGCTTTGTTAGATGACTTTACATGGATGGATTATCTTGATGACGGAAATATATTAGATGACTATGATTTGACTTCCGAAAATGCAATTGTTTCAATTGTTCAAGATCCTAAAAGTATTAATACATCTCTTAATGATGTCTCACAACAGAGTGGCTCTGAGATCAGTGATAAGGATTTCAAGGTTACTGGAGAACAGAATGTAAAACCAAGCTTTTCTTCAGTGAATGCTGAGGAGGACGAGCTCCCATTGCAGGTTCAAAGTTCTGAAATTTTATTGCTTCCATATAAAGAAGATCAGAGTTCTGAAATGTTGTTGCATCCACATACGGAAGGATGTTCCATCACTGGGGAAACCATGGGAGGAGAAGGAGAGGCATCGTCATCAACTGTGGGTTGTGGACAAGATGATTTTGATGGCTTTGGAGACTTATTCAATGAGCCTGAGCCTGAGGTTGTTGCTGGGCCCAGTCCAAGGCCCTCGACTGGTGAGGAAGGTACAGCAACTAGTCTTGTAGCTAGTGAGTCTGATCCTGATGAAGTTGATGATACAAATTCTCCAGTCTCTGTAGAGAGTTGTTTAGCTCATTTCATAAAGCCTGAGCTTCTTGCCAATGAAAATGCTTGGCATTGTGAGAACTGTTCCAAATCTCTGAAGCACCAAAGGTTGGAAGCAAAGAAGCGGCAAAAAGCTGCAACTAAATGTTTGATAAATGGATGTGAGACTAGAGTCCAAAGTGTTTCTCTGAGTTCAGACACTGCTGATATCAGTAATATTAGTAATGGAAATATCAAAAGCAATACTGGTTGCAACCAATTTGGTGAAAACTTGGTTTTGGATGATGGGAAGATGAATTGCTCGAGCGAAAATTGCACATTAGTTGAGAATGCTCTATCAGATAAGATGATTCCTGTTGTTTGTCAACAACAAGAAGGAAACAGTGAGATGAAAGATGTACTTCCAACAGAATCAAATACTTTGGATTGCAATAACAGTGATACTCTAGAAAGTATTAGCAGTCAAGCCATTGATACATGTGCTGATGTGCCTAGTTGTGCTGGATGTGCCTCTGAAAATGCTCCACAGACAAATTCTAAGTTATTAGATGATTGTGAATTAGAAGCAAGTGAAGATGAGGAAATAAATTCCAAACATGTGAAGGTGAAGAGGGATGCGACAAAACGGGTCCTAATTGACAGGGCCCCGCCTATTTTGACCATTCATCTCAAAAGGTTTAGCCAAGATGCTCGTGGTCGCTTAAGTAAATTGAACGGCCATGTCACTTTCAGAGAAAAAATTGAGCTCAGACCATATATGGATTCCAGGTATCTATCTGACCTTTTACAAAGTTCATTTCAGATTTCTAGACACCATAGCAATGTTACTGCTTGTTGTGGTTGGGTAAATCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

955

Amino Acids

103.73

Weight (kDa)

5.35

Isoelectric Point (pI)

49.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	106 - 165	3.6e-13	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	231 - 407	1.1e-24	Ubiquitin carboxyl-terminal hydrolase
UCH	PF00443	702 - 926	7.9e-10	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04860
fragaria_vesca	FvH4_4g16490 FvH4_4g16490
malus_domestica	MD04G1009400.v1.1 MD13G1284700.v1.1
prunus_persica	Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1
pyrus_communis	pycom04g00720 pycom420g01370
rosa_chinensis	RchiOBHm_Chr4g0415171
rosa_laevigata	RLG00000008090
rosa_multiflora	Rmu_sc0003748.1_g000031 Rmu_sc0033472.1_g000001
rosa_roxburghii	Rroxscaffold_5G00359000
rosa_rugosa	Rorug04G0132500 Rorug04G0132500
rosa_samantha	Rh4AG192800 Rh4BG190900 Rh4CG203600 Rh4DG190700
rosa_wichuraiana	Rw4G016390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	802
Acc36I	ACCTGC	2 cut(s)	802, 1699
AccB1I	GGYRCC	1 cut(s)	242
AccB7I	CCANNNNNTGG	1 cut(s)	2122
AciI	CCGC	6 cut(s)	348, 766, 803, 1314, 2137, 2660
AclWI	GGATC	2 cut(s)	1556, 1979
AcoI	YGGCCR	1 cut(s)	2728
AcsI	RAATTY	5 cut(s)	781, 1725, 2008, 2545, 2599
AcuI	CTGAAG	3 cut(s)	192, 1659, 2129
AfaI	GTAC	7 cut(s)	979, 1006, 1090, 1335, 1362, 1954, 2412
AflII	CTTAAG	1 cut(s)	2713
AflIII	ACRYGT	2 cut(s)	2489, 2608
AhlI	ACTAGT	1 cut(s)	1961
AjnI	CCWGG	3 cut(s)	1166, 1329, 2777
AjuI	GAANNNNNNNTTGG	2 cut(s)	1657, 1689
Alw21I	GWGCWC	4 cut(s)	34, 1323, 1701, 2760
Alw26I	GTCTC	4 cut(s)	1600, 1875, 2023, 2171
AlwI	GGATC	2 cut(s)	1556, 1979
AlwNI	CAGNNNCTG	2 cut(s)	2021, 2213
Ama87I	CYCGRG	2 cut(s)	1203, 2314
AoxI	GGCC	4 cut(s)	1919, 1931, 2655, 2728
ApaI	GGGCCC	2 cut(s)	1923, 2659
ApeKI	GCWGC	4 cut(s)	480, 931, 1439, 2147
ApoI	RAATTY	5 cut(s)	781, 1725, 2008, 2545, 2599
AseI	ATTAAT	2 cut(s)	554, 1575
Asp700I	GAANNNNTTC	1 cut(s)	951
AspS9I	GGNCC	7 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656
AsuHPI	GGTGA	8 cut(s)	125, 419, 530, 599, 1328, 1955, 2288, 2629
AsuNHI	GCTAGC	1 cut(s)	644
AvaI	CYCGRG	2 cut(s)	1203, 2314
AvaII	GGWCC	2 cut(s)	800, 2641
AxyI	CCTNAGG	2 cut(s)	1182, 1905
BaeGI	GKGCMC	2 cut(s)	1923, 2659
BanI	GGYRCC	1 cut(s)	242
BanII	GRGCYC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
BarI	GAAGNNNNNNTAC	2 cut(s)	997, 1029
BauI	CACGAG	1 cut(s)	2703
BbsI	GAAGAC	2 cut(s)	198, 1220
Bbv12I	GWGCWC	4 cut(s)	34, 1323, 1701, 2760
BbvCI	CCTCAGC	1 cut(s)	1686
BbvI	GCAGC	4 cut(s)	492, 943, 1451, 2134
BceAI	ACGGC	2 cut(s)	1390, 2743
BciT130I	CCWGG	3 cut(s)	1168, 1331, 2779
BcoDI	GTCTC	4 cut(s)	1600, 1875, 2023, 2171
BcuI	ACTAGT	1 cut(s)	1961
BfmI	CTRYAG	1 cut(s)	2022
BfrI	CTTAAG	1 cut(s)	2713
BfuAI	ACCTGC	2 cut(s)	802, 1699
BglII	AGATCT	1 cut(s)	1144
BisI	GCNGC	5 cut(s)	481, 932, 1440, 2138, 2148
BlsI	GCNGC	5 cut(s)	482, 933, 1441, 2139, 2149
BmcAI	AGTACT	1 cut(s)	1090
Bme1390I	CCNGG	3 cut(s)	1168, 1331, 2779
Bme18I	GGWCC	2 cut(s)	800, 2641
BmeT110I	CYCGRG	2 cut(s)	1203, 2314
BmgT120I	GGNCC	7 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656
BmiI	GGNNCC	7 cut(s)	54, 244, 909, 1921, 2642, 2657, 2658
BmrFI	CCNGG	3 cut(s)	1168, 1331, 2779
BmrI	ACTGGG	3 cut(s)	907, 1812, 1917
BmsI	GCATC	6 cut(s)	1051, 1463, 1783, 1841, 2617, 2689
BmtI	GCTAGC	1 cut(s)	648
BmuI	ACTGGG	3 cut(s)	907, 1812, 1917
BoxI	GACNNNNGTC	1 cut(s)	2183
BpiI	GAAGAC	2 cut(s)	198, 1220
BpmI	CTGGAG	2 cut(s)	1665, 1998
Bpu10I	CCTNAGC	3 cut(s)	1686, 1899, 2055
BsaJI	CCNNGG	3 cut(s)	903, 1812, 1928
BsaXI	ACNNNNNCTCC	4 cut(s)	114, 144, 1996, 2026
Bse21I	CCTNAGG	2 cut(s)	1182, 1905
Bse3DI	GCAATG	2 cut(s)	1703, 2839
BseBI	CCWGG	3 cut(s)	1168, 1331, 2779
BseDI	CCNNGG	3 cut(s)	903, 1812, 1928
BseMI	GCAATG	2 cut(s)	1703, 2839
BseRI	GAGGAG	6 cut(s)	24, 690, 998, 1249, 1703, 1833
BseSI	GKGCMC	2 cut(s)	1923, 2659
BseXI	GCAGC	4 cut(s)	492, 943, 1451, 2134
BseYI	CCCAGC	2 cut(s)	1017, 1916
BsgI	GTGCAG	2 cut(s)	461, 499
BshFI	GGCC	4 cut(s)	1921, 1933, 2657, 2730
BshNI	GGYRCC	1 cut(s)	242
BsiHKAI	GWGCWC	4 cut(s)	34, 1323, 1701, 2760
BsiHKCI	CYCGRG	2 cut(s)	1203, 2314
BsiSI	CCGG	1 cut(s)	798
BslFI	GGGAC	2 cut(s)	1089, 1145
BsmAI	GTCTC	4 cut(s)	1600, 1875, 2023, 2171
BsmFI	GGGAC	2 cut(s)	1089, 1145
BsmI	GAATGC	4 cut(s)	350, 1441, 1687, 2347
BsnI	GGCC	4 cut(s)	1921, 1933, 2657, 2730
BsoBI	CYCGRG	2 cut(s)	1203, 2314
Bsp120I	GGGCCC	2 cut(s)	1919, 2655
Bsp1286I	GDGCHC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
Bsp143I	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
Bsp19I	CCATGG	1 cut(s)	1812
BspACI	CCGC	6 cut(s)	348, 766, 803, 1314, 2137, 2660
BspANI	GGCC	4 cut(s)	1921, 1933, 2657, 2730
BspHI	TCATGA	1 cut(s)	943
BspLI	GGNNCC	7 cut(s)	54, 244, 909, 1921, 2642, 2657, 2658
BspMI	ACCTGC	2 cut(s)	802, 1699
BspOI	GCTAGC	1 cut(s)	648
BspPI	GGATC	2 cut(s)	1556, 1979
BspT107I	GGYRCC	1 cut(s)	242
BspTI	CTTAAG	1 cut(s)	2713
BsrDI	GCAATG	2 cut(s)	1703, 2839
BssECI	CCNNGG	3 cut(s)	903, 1812, 1928
BssMI	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
BssSI	CACGAG	1 cut(s)	2703
BssT1I	CCWWGG	3 cut(s)	903, 1812, 1928
Bst2BI	CACGAG	1 cut(s)	2703
Bst2UI	CCWGG	3 cut(s)	1168, 1331, 2779
Bst4CI	ACNGT	8 cut(s)	459, 941, 1093, 1338, 1846, 2096, 2396, 2450
Bst6I	CTCTTC	2 cut(s)	667, 2615
BstAFI	CTTAAG	1 cut(s)	2713
BstC8I	GCNNGC	3 cut(s)	192, 411, 646
BstDSI	CCRYGG	1 cut(s)	1812
BstKTI	GATC	7 cut(s)	881, 1147, 1253, 1564, 1620, 1753, 1987
BstMAI	GTCTC	4 cut(s)	1600, 1875, 2023, 2171
BstMBI	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
BstMWI	GCNNNNNNNGC	6 cut(s)	96, 415, 477, 1705, 2325, 2519
BstNI	CCWGG	3 cut(s)	1168, 1331, 2779
BstNSI	RCATGY	3 cut(s)	413, 2493, 2612
BstPAI	GACNNNNGTC	1 cut(s)	2183
BstSCI	CCNGG	3 cut(s)	1166, 1329, 2777
BstSFI	CTRYAG	1 cut(s)	2022
BstSLI	GKGCMC	2 cut(s)	1923, 2659
BstV1I	GCAGC	4 cut(s)	492, 943, 1451, 2134
BstV2I	GAAGAC	2 cut(s)	198, 1220
BstX2I	RGATCY	2 cut(s)	1144, 1561
BstYI	RGATCY	2 cut(s)	1144, 1561
Bsu36I	CCTNAGG	2 cut(s)	1182, 1905
BsuRI	GGCC	4 cut(s)	1921, 1933, 2657, 2730
BtgI	CCRYGG	1 cut(s)	1812
BtgZI	GCGATG	1 cut(s)	478
BtsI	GCAGTG	3 cut(s)	625, 1151, 2211
BveI	ACCTGC	2 cut(s)	802, 1699
Cac8I	GCNNGC	3 cut(s)	192, 411, 646
CaiI	CAGNNNCTG	2 cut(s)	2021, 2213
CciI	TCATGA	1 cut(s)	943
Cfr13I	GGNCC	7 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656
CpoI	CGGWCCG	1 cut(s)	800
CseI	GACGC	1 cut(s)	883
CsiI	ACCWGGT	1 cut(s)	1329
Csp6I	GTAC	7 cut(s)	978, 1005, 1089, 1334, 1361, 1953, 2411
CspCI	CAANNNNNGTGG	2 cut(s)	1219, 1254
CspI	CGGWCCG	1 cut(s)	800
CviAII	CATG	9 cut(s)	281, 410, 944, 1418, 1479, 1813, 2490, 2609, 2732
CviQI	GTAC	7 cut(s)	978, 1005, 1089, 1334, 1361, 1953, 2411
DpnI	GATC	7 cut(s)	880, 1146, 1252, 1563, 1619, 1752, 1986
DpnII	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
EaeI	YGGCCR	1 cut(s)	2728
Eam1104I	CTCTTC	2 cut(s)	667, 2615
EarI	CTCTTC	2 cut(s)	667, 2615
Ecl136II	GAGCTC	4 cut(s)	32, 1321, 1699, 2758
Eco130I	CCWWGG	3 cut(s)	903, 1812, 1928
Eco24I	GRGCYC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
Eco32I	GATATC	1 cut(s)	2221
Eco47I	GGWCC	2 cut(s)	800, 2641
Eco53kI	GAGCTC	4 cut(s)	32, 1321, 1699, 2758
Eco57I	CTGAAG	3 cut(s)	192, 1659, 2129
Eco81I	CCTNAGG	2 cut(s)	1182, 1905
Eco88I	CYCGRG	2 cut(s)	1203, 2314
EcoICRI	GAGCTC	4 cut(s)	32, 1321, 1699, 2758
EcoO109I	RGGNCCY	4 cut(s)	1932, 2641, 2655, 2656
EcoRII	CCWGG	3 cut(s)	1166, 1329, 2777
EcoRV	GATATC	1 cut(s)	2221
EcoT14I	CCWWGG	3 cut(s)	903, 1812, 1928
EcoT38I	GRGCYC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
ErhI	CCWWGG	3 cut(s)	903, 1812, 1928
FaeI	CATG	9 cut(s)	284, 413, 947, 1421, 1482, 1816, 2493, 2612, 2735
FalI	AAGNNNNNCTT	6 cut(s)	812, 844, 1657, 1689, 2397, 2429
FaqI	GGGAC	2 cut(s)	1089, 1145
FatI	CATG	9 cut(s)	280, 409, 943, 1417, 1478, 1812, 2489, 2608, 2731
FauI	CCCGC	2 cut(s)	759, 2667
Fnu4HI	GCNGC	5 cut(s)	481, 932, 1440, 2138, 2148
FriOI	GRGCYC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
Fsp4HI	GCNGC	5 cut(s)	481, 932, 1440, 2138, 2148
GluI	GCNGC	5 cut(s)	481, 932, 1440, 2138, 2148
GsaI	CCCAGC	2 cut(s)	1021, 1920
GsuI	CTGGAG	2 cut(s)	1665, 1998
HaeIII	GGCC	4 cut(s)	1921, 1933, 2657, 2730
HapII	CCGG	1 cut(s)	798
HgaI	GACGC	1 cut(s)	883
Hin1II	CATG	9 cut(s)	284, 413, 947, 1421, 1482, 1816, 2493, 2612, 2735
HincII	GTYRAC	1 cut(s)	2378
HindII	GTYRAC	1 cut(s)	2378
HindIII	AAGCTT	3 cut(s)	147, 1220, 1666
HpaII	CCGG	1 cut(s)	798
HphI	GGTGA	8 cut(s)	125, 419, 530, 599, 1328, 1955, 2288, 2629
Hpy166II	GTNNAC	4 cut(s)	772, 978, 1856, 2378
Hpy188III	TCNNGA	8 cut(s)	859, 944, 1203, 1493, 1559, 1988, 2459, 2820
Hpy8I	GTNNAC	4 cut(s)	772, 978, 1856, 2378
Hpy99I	CGWCG	1 cut(s)	574
HpyCH4III	ACNGT	8 cut(s)	459, 941, 1093, 1338, 1846, 2096, 2396, 2450
HpyCH4IV	ACGT	2 cut(s)	1003, 1052
HpyF10VI	GCNNNNNNNGC	6 cut(s)	96, 415, 477, 1705, 2325, 2519
HpySE526I	ACGT	2 cut(s)	1003, 1052
Hsp92II	CATG	9 cut(s)	284, 413, 947, 1421, 1482, 1816, 2493, 2612, 2735
Kzo9I	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
LmnI	GCTCC	4 cut(s)	37, 913, 1704, 2539
Lsp1109I	GCAGC	4 cut(s)	492, 943, 1451, 2134
LweI	GCATC	6 cut(s)	1051, 1463, 1783, 1841, 2617, 2689
MabI	ACCWGGT	1 cut(s)	1329
MaeII	ACGT	2 cut(s)	1003, 1052
MaeIII	GTNAC	8 cut(s)	425, 622, 710, 1112, 1149, 1639, 2734, 2836
MalI	GATC	7 cut(s)	880, 1146, 1252, 1563, 1619, 1752, 1986
MboI	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
MfeI	CAATTG	2 cut(s)	1542, 1551
MflI	RGATCY	2 cut(s)	1144, 1561
MhlI	GDGCHC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
MlyI	GAGTC	5 cut(s)	326, 572, 1142, 1988, 2193
MmeI	TCCRAC	4 cut(s)	352, 552, 2103, 2441
MroXI	GAANNNNTTC	1 cut(s)	951
MslI	CAYNNNNRTG	1 cut(s)	2832
MspA1I	CMGCKG	2 cut(s)	1314, 1400
MspCI	CTTAAG	1 cut(s)	2713
MspI	CCGG	1 cut(s)	798
MspR9I	CCNGG	3 cut(s)	1168, 1331, 2779
MunI	CAATTG	2 cut(s)	1542, 1551
Mva1269I	GAATGC	4 cut(s)	350, 1441, 1687, 2347
MvaI	CCWGG	3 cut(s)	1168, 1331, 2779
MwoI	GCNNNNNNNGC	6 cut(s)	96, 415, 477, 1705, 2325, 2519
NcoI	CCATGG	1 cut(s)	1812
NdeII	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
NheI	GCTAGC	1 cut(s)	644
NlaIII	CATG	9 cut(s)	284, 413, 947, 1421, 1482, 1816, 2493, 2612, 2735
NlaIV	GGNNCC	7 cut(s)	54, 244, 909, 1921, 2642, 2657, 2658
NmuCI	GTSAC	5 cut(s)	425, 622, 1112, 1149, 2734
NspI	RCATGY	3 cut(s)	413, 2493, 2612
PacI	TTAATTAA	1 cut(s)	558
PaeI	GCATGC	1 cut(s)	413
PaeR7I	CTCGAG	2 cut(s)	1203, 2314
PagI	TCATGA	1 cut(s)	943
PaqCI	CACCTGC	1 cut(s)	802
PciI	ACATGT	2 cut(s)	2489, 2608
PctI	GAATGC	4 cut(s)	350, 1441, 1687, 2347
PdmI	GAANNNNTTC	1 cut(s)	951
PfeI	GAWTC	9 cut(s)	62, 305, 855, 1031, 1390, 1409, 2362, 2423, 2774
PflMI	CCANNNNNTGG	1 cut(s)	2122
PkrI	GCNGC	5 cut(s)	482, 933, 1441, 2139, 2149
PleI	GAGTC	5 cut(s)	325, 572, 1141, 1987, 2192
PpsI	GAGTC	5 cut(s)	325, 572, 1141, 1987, 2192
PpuMI	RGGWCCY	1 cut(s)	2641
PscI	ACATGT	2 cut(s)	2489, 2608
PshAI	GACNNNNGTC	1 cut(s)	2183
PshBI	ATTAAT	2 cut(s)	554, 1575
Psp124BI	GAGCTC	4 cut(s)	34, 1323, 1701, 2760
Psp5II	RGGWCCY	1 cut(s)	2641
Psp6I	CCWGG	3 cut(s)	1166, 1329, 2777
PspFI	CCCAGC	2 cut(s)	1017, 1916
PspGI	CCWGG	3 cut(s)	1166, 1329, 2777
PspN4I	GGNNCC	7 cut(s)	54, 244, 909, 1921, 2642, 2657, 2658
PspOMI	GGGCCC	2 cut(s)	1919, 2655
PspPI	GGNCC	7 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656
PspPPI	RGGWCCY	1 cut(s)	2641
PspXI	VCTCGAGB	1 cut(s)	2314
PstNI	CAGNNNCTG	2 cut(s)	2021, 2213
PsuI	RGATCY	2 cut(s)	1144, 1561
PvuII	CAGCTG	1 cut(s)	1400
RsaI	GTAC	7 cut(s)	979, 1006, 1090, 1335, 1362, 1954, 2412
RsaNI	GTAC	7 cut(s)	978, 1005, 1089, 1334, 1361, 1953, 2411
RseI	CAYNNNNRTG	1 cut(s)	2832
Rsr2I	CGGWCCG	1 cut(s)	800
RsrII	CGGWCCG	1 cut(s)	800
SacI	GAGCTC	4 cut(s)	34, 1323, 1701, 2760
SatI	GCNGC	5 cut(s)	481, 932, 1440, 2138, 2148
Sau3AI	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
Sau96I	GGNCC	7 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656
ScaI	AGTACT	1 cut(s)	1090
SchI	GAGTC	5 cut(s)	326, 572, 1142, 1988, 2193
ScrFI	CCNGG	3 cut(s)	1168, 1331, 2779
SduI	GDGCHC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
SexAI	ACCWGGT	1 cut(s)	1329
SfaNI	GCATC	6 cut(s)	1051, 1463, 1783, 1841, 2617, 2689
SfcI	CTRYAG	1 cut(s)	2022
Sfr274I	CTCGAG	2 cut(s)	1203, 2314
SinI	GGWCC	2 cut(s)	800, 2641
SlaI	CTCGAG	2 cut(s)	1203, 2314
SmiMI	CAYNNNNRTG	1 cut(s)	2832
SmlI	CTYRAG	3 cut(s)	1203, 2314, 2713
SmoI	CTYRAG	3 cut(s)	1203, 2314, 2713
SpeI	ACTAGT	1 cut(s)	1961
SphI	GCATGC	1 cut(s)	413
SsiI	CCGC	6 cut(s)	348, 766, 803, 1314, 2137, 2660
SspI	AATATT	1 cut(s)	2230
SstI	GAGCTC	4 cut(s)	34, 1323, 1701, 2760
StyD4I	CCNGG	3 cut(s)	1166, 1329, 2777
StyI	CCWWGG	3 cut(s)	903, 1812, 1928
TaaI	ACNGT	8 cut(s)	459, 941, 1093, 1338, 1846, 2096, 2396, 2450
TaiI	ACGT	2 cut(s)	1006, 1055
TaqI	TCGA	5 cut(s)	594, 1204, 1249, 1937, 2315
TatI	WGTACW	3 cut(s)	977, 1088, 2410
TauI	GCSGC	1 cut(s)	2140
TfiI	GAWTC	9 cut(s)	62, 305, 855, 1031, 1390, 1409, 2362, 2423, 2774
TseFI	GTSAC	5 cut(s)	425, 622, 1112, 1149, 2734
TseI	GCWGC	4 cut(s)	480, 931, 1439, 2147
Tsp45I	GTSAC	5 cut(s)	425, 622, 1112, 1149, 2734
TspGWI	ACGGA	2 cut(s)	1515, 1799
Van91I	CCANNNNNTGG	1 cut(s)	2122
Vha464I	CTTAAG	1 cut(s)	2713
VpaK11BI	GGWCC	2 cut(s)	800, 2641
VspI	ATTAAT	2 cut(s)	554, 1575
XapI	RAATTY	5 cut(s)	781, 1725, 2008, 2545, 2599
XbaI	TCTAGA	2 cut(s)	2458, 2819
XceI	RCATGY	3 cut(s)	413, 2493, 2612
XcmI	CCANNNNNNNNNTGG	2 cut(s)	462, 2702
XhoI	CTCGAG	2 cut(s)	1203, 2314
XmnI	GAANNNNTTC	1 cut(s)	951
ZrmI	AGTACT	1 cut(s)	1090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.