Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008431

Physical Location & Seq

Genomic Coordinates Reverse (-)

42879279 .. 42889298

Gene Structure 10020 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1284700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2931 bp

ATGGGGAAGAAAGTTAAGAAGAAGGCTAGAGCTCCTCCAAGGGAGAAGTTGGTTGCAGCCGATTCCCCAAAAAAAGCTCCCCAACCATGCAACCCAAGTGTTAAGGATGGTGACAATGGAGTTTCAGTTGCGAAAGAGAGAAGGCCTTGTCCTCATGTTGACAAAGGTGTAAATTTGGATAAATTGTCTGCTAAAATTGGGTCTTCAGGACCTGTTAGGTGTGAATATTGCAGGGAAGGAGCGCTTGATAGGAGGGGTGGTAAAGGAAAGGGAAAACATGGGAAGAAGAAAGGGAGCTCGTCCGTGGATTCAAAATCGGAGTCCAAAGCCATTTGGGTTTGTTTGGAGTGTGGTCATTATTCTTGCGGTGGGTTTGGACTTCATACAACCCCTCAATGCCATGCAGTTCGGCATGCTAGGCAAACTCAGCACCCAATAGTGATCCACTTTGAAAAGCCGCAGATGCGATGGTGCTTCCCGTGCAAAATGCTTATTACAATTGACAAGACAGAAGAGGATGGTGAACAAAAAGATGTGTTTGCGGATGTTGTTAAATTGATAAAGGGTCATTCATCGGAGGAATCATTGGTTAATGCTGAGGATGTGTGGTTTGTGAGTGGCAGTGTGACAAGCGAAATCAAATCAGCTAGCAATATATCATGTGATTTGGATGGAAGAGTTGGTTATACAGTGAGAGGCTTGGTTAATCTTGGTAACACTTGCTTCTTTAATTCTGTCTTACAGAACCTTCTAGCCATGGATAGGTTGCGGGGCTACTTCTTAAATTTAGATGCATCTACTGGAGCTCTTACTGTTTCCTTGAAGAAACTCTTTACTGAAGCAAAACCAGAGTCAGGAGTTAAGAATGTGATAAACCCAAGGTCACTTTTTGGGTGTCTTTGTTCCAAGGCTCCCCAATTTAAGGGGTATCAGCAGCAAGACAGCCATGAATTGCTTCATTGCTTACTGGATGAATTGTGCATTGAGGAGTTGAGTACGAGGAAACGTATAAATTCTTCTCAGGAAAATGGGAATCCTTCAAATTCAGGTCCTACTTTTGTGGATACTGCATTTGGGGGCCAAATAGCAAGTACAGTTCGCTGTGTCGAATGTGGTCACTCCTCAACTGTGTACGAGTCATTTTTAGATCTGTCATTGTCAGTTCCAACCAGAAAATCCCCATCTAAGACTGCCCAACCCACCTCTCGAACAAAGAAGATGAAGTTGCCGCCCAAGAAGAGTGGAAAGTTTCGGTCTAAAAATAACGAAGAGAAGAATTCTGTACCATCACCAAGTGTTCCAACCCCATCAACCAGCAGTGAGGTTTCTGAACAAAAGGAATTAGTTGTAAATAGTCTATCTGCTGTCCAAGAATCTGCAGATGATGTCTGTGAGGATGCAGCAGAGCAAACATCACCTTTGTTAGATGACTGTTCATGGTTGGATTATCTTGATTCGGGAAATATGTTAGATGACAATGATTTATCTTCGCAACATAATGATATTTCAATTGTTCAAGATTCTGAAAAAAAAAATACACATCTTAATGATGTCTCTTTACAGAGTGGCTCTGAATCTAGTAATCAGGTTTTTACGCCTAACGGGGAACCAAATGTAAAACCTGACTTTTCTTCAGTGAATTCTAGGGAGGAAGAGCTCCCATTACAGGTTCTGGATTCTGAAGTTTTGTTGCTTCCGTATAAAGCAGAGTGTTCTATCACTGAGGAAATCATGGGAGGCGAAGGTGAGGCCTCCTCCTTGGTTGTGGGTTGTGGACAAGATGACTTTGGCTTTGGGGATTTATTCAACGAACCTGAAGTTTATGGCCCCCCTAGAAGACCCTCTGTAGGTGAGGGTGGCACAGATACTAGTATCATAGCTAGCGAGTCTGATCCAGATGAAGTTGATGATACTGTTTCTCCAGTGTCTGTAGAGAGTTGTTTGGCTCATTTCATAAAGCCTGAGCTTCTTGCCAGTGAAAATGCTTGGCATTGTGAGAACTGTTCCAAAACTCTTCCAAGCCAAAAGTTGGAAGAAAAGAAGCAGGTAAAATCTGCTGCTAGAGTTTTGTTAAATGGATGTGAGACTAGAACACAAAGTGATTCACTGAGTTTAAATACGGGTCCGTGGCCTGCCGATGTCAGAACGCTTGGTAATGGGAATGTCAAAAGCAATACTGGTTGTAACCAGTTTGGTGAAAACTTGATTTTGCATGATGGAAAAATAAATTGCTTGAGCCAAAATTGCTCAGCAGTTGAGAATGGTCAATCACATAAGTTGAATTCTGTTGTTTGTCGACGACAAGATGAGATTAAAGATGCTCCTCCAGTGCAATCAAATACTTCAGATTGCAATAACGCTTGCAGCCTAGAAAGTTTCAGTGATCAAGTCATTGATTCTAGGGCTGATGAGTCTAGAAGTGCTAGTTTCACCTCTGACACTGTACCGCAGACTAATGATGGGATATTGGATGGACATCGTGAATCAGAAGAAAGTGAGGATGAGGAAATAAATGCCAAACGTGTGAATTTGAAGAGGAATGCGACTAAAAGGTACCTCATCAACAGGGCCCCACCGATTTTGACTATTCATCTCAAGAGGTTCAGCCAAGATGCTCGTGGTCACTTGAGTAAATTGAATGGCCATGTCAGTTTTCGAGAAAAATTTGATCTTAGGCCATATATGGATTCCAGTTCCACAGATGGGGAGAAGTATGAATACCAGCTATTGGGGGTTGTGGAGCATTCTGGAACCATGAGAGGGGGCCACTATGTTGCATACGTGAGAGGGGGTGAAAGGGGCAGAGGAAAAGCTGAGAAAGAAAATATCGGTCATATGTGGTATTATGCTAGTGATGCACATGTGCGTCAGGTTTCCCTAGATGAGGTTCTTCGCTCTGATGCCTATATCTTATTCTATGAAAAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

977

Amino Acids

106.97

Weight (kDa)

5.75

Isoelectric Point (pI)

48.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	107 - 166	4.9e-12	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	232 - 973	1.2e-55	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04860
fragaria_vesca	FvH4_4g16490 FvH4_4g16490
malus_domestica	MD04G1009400.v1.1 MD13G1284700.v1.1
prunus_persica	Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1
pyrus_communis	pycom04g00720 pycom420g01370
rosa_chinensis	RchiOBHm_Chr4g0415171
rosa_laevigata	RLG00000008090
rosa_multiflora	Rmu_sc0003748.1_g000031 Rmu_sc0033472.1_g000001
rosa_roxburghii	Rroxscaffold_5G00359000
rosa_rugosa	Rorug04G0132500 Rorug04G0132500
rosa_samantha	Rh4AG192800 Rh4BG190900 Rh4CG203600 Rh4DG190700
rosa_wichuraiana	Rw4G016390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2035
Acc65I	GGTACC	1 cut(s)	2553
AccB1I	GGYRCC	1 cut(s)	2553
AccB7I	CCANNNNNTGG	2 cut(s)	2029, 2728
AccI	GTMKAC	1 cut(s)	2296
AciI	CCGC	6 cut(s)	366, 458, 542, 769, 1229, 2447
AclWI	GGATC	2 cut(s)	436, 1886
AcoI	YGGCCR	1 cut(s)	2641
AcsI	RAATTY	9 cut(s)	172, 784, 1012, 1042, 1276, 1639, 2281, 2527, 2663
AcuI	CTGAAG	6 cut(s)	189, 858, 1617, 1701, 1836, 2328
AdeI	CACNNNGTG	2 cut(s)	1295, 2099
AfaI	GTAC	6 cut(s)	997, 1093, 1133, 1284, 2445, 2555
AfeI	AGCGCT	1 cut(s)	243
AflIII	ACRYGT	2 cut(s)	2521, 2860
AhlI	ACTAGT	1 cut(s)	1868
Alw21I	GWGCWC	4 cut(s)	34, 299, 808, 1659
Alw26I	GTCTC	2 cut(s)	1558, 2078
AlwI	GGATC	2 cut(s)	436, 1886
AlwNI	CAGNNNCTG	3 cut(s)	212, 1672, 1928
Aor51HI	AGCGCT	1 cut(s)	243
AoxI	GGCC	9 cut(s)	143, 1078, 1749, 1825, 2129, 2568, 2641, 2675, 2764
ApaI	GGGCCC	1 cut(s)	2572
ApeKI	GCWGC	5 cut(s)	56, 934, 1400, 2057, 2364
ApoI	RAATTY	9 cut(s)	172, 784, 1012, 1042, 1276, 1639, 2281, 2527, 2663
ArsI	GACNNNNNNTTYG	6 cut(s)	305, 337, 836, 868, 1775, 1807
Asp700I	GAANNNNTTC	1 cut(s)	954
Asp718I	GGTACC	1 cut(s)	2553
AspLEI	GCGC	1 cut(s)	244
AspS9I	GGNCC	8 cut(s)	209, 1049, 1078, 1826, 2123, 2568, 2569, 2764
AsuHPI	GGTGA	9 cut(s)	122, 533, 1281, 1407, 1757, 1862, 2207, 2422, 2804
AsuNHI	GCTAGC	2 cut(s)	647, 1880
AvaII	GGWCC	3 cut(s)	209, 1049, 2123
BaeGI	GKGCMC	1 cut(s)	2572
BalI	TGGCCA	1 cut(s)	2643
BanI	GGYRCC	1 cut(s)	2553
BanII	GRGCYC	5 cut(s)	34, 299, 808, 1659, 2572
BarI	GAAGNNNNNNTAC	4 cut(s)	1000, 1032, 2702, 2734
BauI	CACGAG	1 cut(s)	2616
BbsI	GAAGAC	2 cut(s)	195, 1843
Bbv12I	GWGCWC	4 cut(s)	34, 299, 808, 1659
BbvCI	CCTCAGC	1 cut(s)	597
BbvI	GCAGC	5 cut(s)	68, 946, 1412, 2044, 2376
BciVI	GTATCC	1 cut(s)	1057
BclI	TGATCA	1 cut(s)	2383
BcoDI	GTCTC	2 cut(s)	1558, 2078
BcuI	ACTAGT	1 cut(s)	1868
BfmI	CTRYAG	3 cut(s)	1377, 1845, 1929
BfoI	RGCGCY	1 cut(s)	245
BfuAI	ACCTGC	1 cut(s)	2035
BfuI	GTATCC	1 cut(s)	1057
BglII	AGATCT	1 cut(s)	1147
BisI	GCNGC	7 cut(s)	57, 458, 935, 1229, 1401, 2058, 2365
BlpI	GCTNAGC	1 cut(s)	2248
BlsI	GCNGC	7 cut(s)	58, 459, 936, 1230, 1402, 2059, 2366
Bme18I	GGWCC	3 cut(s)	209, 1049, 2123
BmgT120I	GGNCC	8 cut(s)	209, 1049, 1078, 1826, 2123, 2568, 2569, 2764
BmsI	GCATC	8 cut(s)	453, 781, 803, 1387, 2308, 2602, 2845, 2890
BmtI	GCTAGC	2 cut(s)	651, 1884
BpiI	GAAGAC	2 cut(s)	195, 1843
BplI	GAGNNNNNCTC	2 cut(s)	1739, 1771
BpmI	CTGGAG	3 cut(s)	822, 1905, 2310
Bpu10I	CCTNAGC	2 cut(s)	597, 1962
Bpu1102I	GCTNAGC	1 cut(s)	2248
BpuEI	CTTGAG	3 cut(s)	2254, 2579, 2647
BsaAI	YACGTR	1 cut(s)	2782
BsaBI	GATNNNNATC	1 cut(s)	2475
BsaJI	CCNNGG	7 cut(s)	38, 303, 756, 878, 906, 1758, 2126
BsaXI	ACNNNNNCTCC	6 cut(s)	111, 141, 244, 274, 1903, 1933
Bse1I	ACTGG	8 cut(s)	805, 972, 1922, 1974, 2182, 2188, 2327, 2691
Bse3DI	GCAATG	1 cut(s)	958
Bse8I	GATNNNNATC	1 cut(s)	2475
BseDI	CCNNGG	7 cut(s)	38, 303, 756, 878, 906, 1758, 2126
BseJI	GATNNNNATC	1 cut(s)	2475
BseMI	GCAATG	1 cut(s)	958
BseMII	CTCAG	8 cut(s)	440, 588, 1034, 1713, 1953, 2099, 2262, 2805
BseNI	ACTGG	8 cut(s)	805, 972, 1922, 1974, 2182, 2188, 2327, 2691
BseRI	GAGGAG	5 cut(s)	24, 1001, 1111, 1744, 2313
BseSI	GKGCMC	1 cut(s)	2572
BseXI	GCAGC	5 cut(s)	68, 946, 1412, 2044, 2376
BshFI	GGCC	9 cut(s)	145, 1080, 1751, 1827, 2131, 2570, 2643, 2677, 2766
BshNI	GGYRCC	1 cut(s)	2553
BsiHKAI	GWGCWC	4 cut(s)	34, 299, 808, 1659
BsmAI	GTCTC	2 cut(s)	1558, 2078
BsmI	GAATGC	2 cut(s)	2545, 2743
BsnI	GGCC	9 cut(s)	145, 1080, 1751, 1827, 2131, 2570, 2643, 2677, 2766
Bsp120I	GGGCCC	1 cut(s)	2568
Bsp1286I	GDGCHC	5 cut(s)	34, 299, 808, 1659, 2572
Bsp143I	GATC	5 cut(s)	441, 1147, 1891, 2383, 2668
Bsp1720I	GCTNAGC	1 cut(s)	2248
Bsp19I	CCATGG	1 cut(s)	756
BspACI	CCGC	6 cut(s)	366, 458, 542, 769, 1229, 2447
BspANI	GGCC	9 cut(s)	145, 1080, 1751, 1827, 2131, 2570, 2643, 2677, 2766
BspCNI	CTCAG	8 cut(s)	439, 589, 1033, 1714, 1954, 2100, 2261, 2806
BspMAI	CTGCAG	1 cut(s)	1381
BspMI	ACCTGC	1 cut(s)	2035
BspOI	GCTAGC	2 cut(s)	651, 1884
BspPI	GGATC	2 cut(s)	436, 1886
BspQI	GCTCTTC	1 cut(s)	1647
BspT107I	GGYRCC	1 cut(s)	2553
BsrDI	GCAATG	1 cut(s)	958
BsrI	ACTGG	8 cut(s)	805, 972, 1922, 1974, 2182, 2188, 2327, 2691
BssECI	CCNNGG	7 cut(s)	38, 303, 756, 878, 906, 1758, 2126
BssMI	GATC	5 cut(s)	441, 1147, 1891, 2383, 2668
BssSI	CACGAG	1 cut(s)	2616
BssT1I	CCWWGG	5 cut(s)	38, 756, 878, 906, 1758
Bst2BI	CACGAG	1 cut(s)	2616
Bst4CI	ACNGT	8 cut(s)	691, 814, 1096, 1129, 1433, 1915, 2003, 2443
Bst6I	CTCTTC	7 cut(s)	507, 670, 1232, 1263, 1647, 2019, 2528
BstBAI	YACGTR	1 cut(s)	2782
BstC8I	GCNNGC	5 cut(s)	414, 649, 1882, 2133, 2362
BstDSI	CCRYGG	3 cut(s)	303, 756, 2126
BstENI	CCTNNNNNAGG	1 cut(s)	2882
BstH2I	RGCGCY	1 cut(s)	245
BstHHI	GCGC	1 cut(s)	244
BstKTI	GATC	5 cut(s)	444, 1150, 1894, 2386, 2671
BstMAI	GTCTC	2 cut(s)	1558, 2078
BstMBI	GATC	5 cut(s)	441, 1147, 1891, 2383, 2668
BstMWI	GCNNNNNNNGC	6 cut(s)	418, 427, 463, 480, 2244, 2855
BstNSI	RCATGY	2 cut(s)	416, 2864
BstSFI	CTRYAG	3 cut(s)	1377, 1845, 1929
BstSLI	GKGCMC	1 cut(s)	2572
BstV1I	GCAGC	5 cut(s)	68, 946, 1412, 2044, 2376
BstV2I	GAAGAC	2 cut(s)	195, 1843
BstX2I	RGATCY	1 cut(s)	1147
BstYI	RGATCY	1 cut(s)	1147
BsuI	GTATCC	1 cut(s)	1057
BsuRI	GGCC	9 cut(s)	145, 1080, 1751, 1827, 2131, 2570, 2643, 2677, 2766
BtgI	CCRYGG	3 cut(s)	303, 756, 2126
BtgZI	GCGATG	1 cut(s)	481
BtsI	GCAGTG	2 cut(s)	628, 1324
BveI	ACCTGC	1 cut(s)	2035
Cac8I	GCNNGC	5 cut(s)	414, 649, 1882, 2133, 2362
CaiI	CAGNNNCTG	3 cut(s)	212, 1672, 1928
CfoI	GCGC	1 cut(s)	244
Cfr13I	GGNCC	8 cut(s)	209, 1049, 1078, 1826, 2123, 2568, 2569, 2764
CseI	GACGC	1 cut(s)	2855
Csp6I	GTAC	6 cut(s)	996, 1092, 1132, 1283, 2444, 2554
CspCI	CAANNNNNGTGG	4 cut(s)	1222, 1257, 2562, 2597
CviQI	GTAC	6 cut(s)	996, 1092, 1132, 1283, 2444, 2554
DpnI	GATC	5 cut(s)	443, 1149, 1893, 2385, 2670
DpnII	GATC	5 cut(s)	441, 1147, 1891, 2383, 2668
DraI	TTTAAA	1 cut(s)	2115
DraIII	CACNNNGTG	2 cut(s)	1295, 2099
EaeI	YGGCCR	1 cut(s)	2641
Eam1104I	CTCTTC	7 cut(s)	507, 670, 1232, 1263, 1647, 2019, 2528
EarI	CTCTTC	7 cut(s)	507, 670, 1232, 1263, 1647, 2019, 2528
Ecl136II	GAGCTC	4 cut(s)	32, 297, 806, 1657
Eco130I	CCWWGG	5 cut(s)	38, 756, 878, 906, 1758
Eco147I	AGGCCT	2 cut(s)	145, 1751
Eco24I	GRGCYC	5 cut(s)	34, 299, 808, 1659, 2572
Eco47I	GGWCC	3 cut(s)	209, 1049, 2123
Eco47III	AGCGCT	1 cut(s)	243
Eco53kI	GAGCTC	4 cut(s)	32, 297, 806, 1657
Eco57I	CTGAAG	6 cut(s)	189, 858, 1617, 1701, 1836, 2328
EcoICRI	GAGCTC	4 cut(s)	32, 297, 806, 1657
EcoNI	CCTNNNNNAGG	1 cut(s)	2882
EcoO109I	RGGNCCY	4 cut(s)	209, 1049, 2568, 2569
EcoRI	GAATTC	3 cut(s)	1276, 1639, 2281
EcoT14I	CCWWGG	5 cut(s)	38, 756, 878, 906, 1758
EcoT22I	ATGCAT	1 cut(s)	796
EcoT38I	GRGCYC	5 cut(s)	34, 299, 808, 1659, 2572
ErhI	CCWWGG	5 cut(s)	38, 756, 878, 906, 1758
FalI	AAGNNNNNCTT	4 cut(s)	228, 260, 815, 847
FauI	CCCGC	1 cut(s)	762
FauNDI	CATATG	1 cut(s)	2835
FbaI	TGATCA	1 cut(s)	2383
FblI	GTMKAC	1 cut(s)	2296
Fnu4HI	GCNGC	7 cut(s)	57, 458, 935, 1229, 1401, 2058, 2365
FriOI	GRGCYC	5 cut(s)	34, 299, 808, 1659, 2572
Fsp4HI	GCNGC	7 cut(s)	57, 458, 935, 1229, 1401, 2058, 2365
GlaI	GCGC	1 cut(s)	243
GluI	GCNGC	7 cut(s)	57, 458, 935, 1229, 1401, 2058, 2365
GsuI	CTGGAG	3 cut(s)	822, 1905, 2310
HaeII	RGCGCY	1 cut(s)	245
HaeIII	GGCC	9 cut(s)	145, 1080, 1751, 1827, 2131, 2570, 2643, 2677, 2766
HgaI	GACGC	1 cut(s)	2855
HhaI	GCGC	1 cut(s)	244
Hin6I	GCGC	1 cut(s)	242
HinP1I	GCGC	1 cut(s)	242
HincII	GTYRAC	2 cut(s)	160, 2297
HindII	GTYRAC	2 cut(s)	160, 2297
HphI	GGTGA	9 cut(s)	122, 533, 1281, 1407, 1757, 1862, 2207, 2422, 2804
Hpy166II	GTNNAC	5 cut(s)	160, 524, 1132, 1775, 2297
Hpy8I	GTNNAC	5 cut(s)	160, 524, 1132, 1775, 2297
Hpy99I	CGWCG	1 cut(s)	2301
HpyAV	CCTTC	6 cut(s)	16, 135, 230, 758, 1047, 1736
HpyCH4III	ACNGT	8 cut(s)	691, 814, 1096, 1129, 1433, 1915, 2003, 2443
HpyCH4IV	ACGT	3 cut(s)	1006, 2521, 2781
HpyF10VI	GCNNNNNNNGC	6 cut(s)	418, 427, 463, 480, 2244, 2855
HpySE526I	ACGT	3 cut(s)	1006, 2521, 2781
HspAI	GCGC	1 cut(s)	242
KpnI	GGTACC	1 cut(s)	2557
Ksp22I	TGATCA	1 cut(s)	2383
Kzo9I	GATC	5 cut(s)	441, 1147, 1891, 2383, 2668
LguI	GCTCTTC	1 cut(s)	1647
LmnI	GCTCC	9 cut(s)	37, 82, 239, 294, 803, 916, 1662, 2326, 2740
Lsp1109I	GCAGC	5 cut(s)	68, 946, 1412, 2044, 2376
LweI	GCATC	8 cut(s)	453, 781, 803, 1387, 2308, 2602, 2845, 2890
MaeII	ACGT	3 cut(s)	1006, 2521, 2781
MaeIII	GTNAC	7 cut(s)	110, 625, 713, 882, 1115, 2183, 2621
MalI	GATC	5 cut(s)	443, 1149, 1893, 2385, 2670
MboI	GATC	5 cut(s)	441, 1147, 1891, 2383, 2668
MfeI	CAATTG	2 cut(s)	498, 1509
MflI	RGATCY	1 cut(s)	1147
MhlI	GDGCHC	5 cut(s)	34, 299, 808, 1659, 2572
MlsI	TGGCCA	1 cut(s)	2643
MluNI	TGGCCA	1 cut(s)	2643
MlyI	GAGTC	5 cut(s)	329, 860, 1145, 1895, 2420
MmeI	TCCRAC	4 cut(s)	1190, 1325, 1422, 2010
Mox20I	TGGCCA	1 cut(s)	2643
Mph1103I	ATGCAT	1 cut(s)	796
MroXI	GAANNNNTTC	1 cut(s)	954
MscI	TGGCCA	1 cut(s)	2643
MslI	CAYNNNNRTG	1 cut(s)	1545
Msp20I	TGGCCA	1 cut(s)	2643
MunI	CAATTG	2 cut(s)	498, 1509
Mva1269I	GAATGC	2 cut(s)	2545, 2743
MwoI	GCNNNNNNNGC	6 cut(s)	418, 427, 463, 480, 2244, 2855
NcoI	CCATGG	1 cut(s)	756
NdeI	CATATG	1 cut(s)	2835
NdeII	GATC	5 cut(s)	441, 1147, 1891, 2383, 2668
NheI	GCTAGC	2 cut(s)	647, 1880
NmuCI	GTSAC	5 cut(s)	110, 625, 882, 1115, 2621
NsiI	ATGCAT	1 cut(s)	796
NspI	RCATGY	2 cut(s)	416, 2864
PaeI	GCATGC	1 cut(s)	416
PceI	AGGCCT	2 cut(s)	145, 1751
PciI	ACATGT	1 cut(s)	2860
PciSI	GCTCTTC	1 cut(s)	1647
PctI	GAATGC	2 cut(s)	2545, 2743
PdmI	GAANNNNTTC	1 cut(s)	954
PflMI	CCANNNNNTGG	2 cut(s)	2029, 2728
PkrI	GCNGC	7 cut(s)	58, 459, 936, 1230, 1402, 2059, 2366
PleI	GAGTC	5 cut(s)	328, 859, 1144, 1894, 2419
PpsI	GAGTC	5 cut(s)	328, 859, 1144, 1894, 2419
Ppu21I	YACGTR	1 cut(s)	2782
PpuMI	RGGWCCY	2 cut(s)	209, 1049
PscI	ACATGT	1 cut(s)	2860
Psp124BI	GAGCTC	4 cut(s)	34, 299, 808, 1659
Psp5II	RGGWCCY	2 cut(s)	209, 1049
PspOMI	GGGCCC	1 cut(s)	2568
PspPI	GGNCC	8 cut(s)	209, 1049, 1078, 1826, 2123, 2568, 2569, 2764
PspPPI	RGGWCCY	2 cut(s)	209, 1049
PstI	CTGCAG	1 cut(s)	1381
PstNI	CAGNNNCTG	3 cut(s)	212, 1672, 1928
PsuI	RGATCY	1 cut(s)	1147
RsaI	GTAC	6 cut(s)	997, 1093, 1133, 1284, 2445, 2555
RsaNI	GTAC	6 cut(s)	996, 1092, 1132, 1283, 2444, 2554
RseI	CAYNNNNRTG	1 cut(s)	1545
SacI	GAGCTC	4 cut(s)	34, 299, 808, 1659
SalI	GTCGAC	1 cut(s)	2295
SapI	GCTCTTC	1 cut(s)	1647
SatI	GCNGC	7 cut(s)	57, 458, 935, 1229, 1401, 2058, 2365
Sau3AI	GATC	5 cut(s)	441, 1147, 1891, 2383, 2668
Sau96I	GGNCC	8 cut(s)	209, 1049, 1078, 1826, 2123, 2568, 2569, 2764
SchI	GAGTC	5 cut(s)	329, 860, 1145, 1895, 2420
SduI	GDGCHC	5 cut(s)	34, 299, 808, 1659, 2572
SfaNI	GCATC	8 cut(s)	453, 781, 803, 1387, 2308, 2602, 2845, 2890
SfcI	CTRYAG	3 cut(s)	1377, 1845, 1929
SinI	GGWCC	3 cut(s)	209, 1049, 2123
SmiMI	CAYNNNNRTG	1 cut(s)	1545
SmlI	CTYRAG	3 cut(s)	2233, 2594, 2626
SmoI	CTYRAG	3 cut(s)	2233, 2594, 2626
SpeI	ACTAGT	1 cut(s)	1868
SphI	GCATGC	1 cut(s)	416
SseBI	AGGCCT	2 cut(s)	145, 1751
SsiI	CCGC	6 cut(s)	366, 458, 542, 769, 1229, 2447
SspI	AATATT	1 cut(s)	227
SstI	GAGCTC	4 cut(s)	34, 299, 808, 1659
StuI	AGGCCT	2 cut(s)	145, 1751
StyI	CCWWGG	5 cut(s)	38, 756, 878, 906, 1758
TaaI	ACNGT	8 cut(s)	691, 814, 1096, 1129, 1433, 1915, 2003, 2443
TaiI	ACGT	3 cut(s)	1009, 2524, 2784
TaqI	TCGA	4 cut(s)	1107, 1207, 2296, 2656
TaqII	GACCGA	2 cut(s)	1242, 2819
TatI	WGTACW	1 cut(s)	1091
TauI	GCSGC	2 cut(s)	460, 1231
TseFI	GTSAC	5 cut(s)	110, 625, 882, 1115, 2621
TseI	GCWGC	5 cut(s)	56, 934, 1400, 2057, 2364
Tsp45I	GTSAC	5 cut(s)	110, 625, 882, 1115, 2621
TspGWI	ACGGA	3 cut(s)	292, 1686, 2115
Van91I	CCANNNNNTGG	2 cut(s)	2029, 2728
VpaK11BI	GGWCC	3 cut(s)	209, 1049, 2123
XagI	CCTNNNNNAGG	1 cut(s)	2882
XapI	RAATTY	9 cut(s)	172, 784, 1012, 1042, 1276, 1639, 2281, 2527, 2663
XbaI	TCTAGA	1 cut(s)	2414
XceI	RCATGY	2 cut(s)	416, 2864
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2615
XmiI	GTMKAC	1 cut(s)	2296
XmnI	GAANNNNTTC	1 cut(s)	954
Zsp2I	ATGCAT	1 cut(s)	796

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.