Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0008431

Physical Location & Seq

Genomic Coordinates Reverse (-)

43010349 .. 43016829

Gene Structure 6481 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG203600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3018 bp

ATGGGGAAGAAGGTTAAGAAGAAGACTAGAGCTCCTCAAAAGGAGAAGTGGGTTCCATCTGATTCCCCAAAGAAGGTTGCTGAACAAAGCAACCCAAGCGTTAAGGATGATGGTGATGATGGAGTTTCAGTTGCAAAAGTGAGAAAAGCTTGTCCTCACATTGACAAAGGTGTTGATTTGAATAAATTGCAAGCTAAAATTGGGTCTTCAGCAGTTGTTAGGTGTGAAGATTGTAGGGAGGGTGCCATTGATAGGAAGGGGGGTAAAGGAAAGGGCAAACATGGTAAGAAGAAAGGAGGTGCTGATTCTAAATCCGAGTCAAAATCTGTATGGCTTTGTTTAGAATGCGGTCATTTTGCTTGTGGTGGGGTTGGACTTCCAATAACCCCTCAGTGCCACGCAGTTCGGCATGCAAGGCAAACTCGTCACCCATTGGTGGTGCAGTTTGAAAACCCACAGTTGCGATGGTGCTTCCAGTGCAGCACACTTATTACCATTGATAAGACGGGGGATAATGGTGAAGAAAAAGATGTCTTTTCCGATGTTGTGAAATTAATTAAGGGTCATTCATCGGAGGACTCATCAGGTGATGTTGAGGATGTATGGTTTGGTAGTGGCAGTGTGACAAGCGAAATCAAATCAGCTAGCAATACATCAAGCCTTTTGGATGGAAGAGGAGGTTATGCAGTAAGAGGATTAGTTAATCTTGGTAACACTTGCTTCTTCAATTCTATTCTACAGAACCTTCTAGCTATTGATAGGTTGCGGGTTTACTTCTTAAATTTTGAAGCACCTGCCGGACCGCTTACTATTTCCTTGAAGAAACTCTTTACTGAAACAAAACCAGAGGCAGGATTCAAGAATGTGATAAACCCTAGATCGTTTTTCGGTTGTGTCTGTTCCAAGGCTCCCCAGTTTAGGGGCTATCAGCAGCAAGACAGTCATGAATTGCTTCGTTGCTTACTGGATGGATTGTGTACTGAGGAGTTGAGTATGAGGAAACGTACAAGTTCTTCCCAGCAAAATGAGGATTCATCAAATCCAGTTCCTACGTTTGTTGACACTGTATTTGGGGGACAGGTATCAAGTACTGTTCGCTGTGTTGAATGTGGTCACTCATCAACTATCTATGAGTCATTCTTAGATCTGTCACTGCCAGTCCCAACCAGGAAATCCCCACCTAAGGCTTCCCTACCAAGCTCTCGAGCAAAGAAGACGAAGCTTCCACCCAAGAGGAGTGGAAAGGTTCGATCTAAAATTAACAAAGACAAGAACTCTGCAACATCTCCAAGTGTGGCAACCACATCAACCAGCGGTGAGCTCTCTAACCAGGTACAGTCAAGTTCTACTGAGCTAAATGTACCTGAACAAAACGGCTTGGTTATGAAGAATCTATCAGCTGTCCAAGAATCTCAACATGAACAAGTCTTTGAGAATGCAGCAGGAGAACAAGCATCAGCTTTGTTAGATGACTTTACATGGATGGATTATCTTGATGACGGAAATATATTAGATGACTATGATTTGACTTCAGAAAATGCTATTGTTTCAATTGTTCAAGATCCTAAAAGTATTAATACATCTCTTAATGATGTCTCACAACAGAGTGGCTCTGAGATCAGTGATAAAGATTTCAAGGTTACTGGAGAACAGAATGTAAAACCAAACTTTTCTTCAGTGAATGCTGAGGAGGACGAGCTCCCATTGCAGGTTCAAAGTTCTGAAATTTTATTGCTTCCATATAAAGAAGATCAGAGTTCTGAAATGTTGTTGCTTCCACATAAGGAAGGATGCTCCATCACTGGGGAAATCATGGGAGGAGAAGGAGAGGCATCGTCATCAACTGTGGGTTGTGGACAAGATGATTTTGATGGCTTTGGAGACTTATTCAATGAGCCTGAGCCTGAGGTTGTTGCTGGGCCCAGTCCAAGGCCCTCTACTGGTGAGGAAGGTACAGCAACTAGTCTTGCAGCTAGTGAGTCTGATCCTGATGAAGTTGATGATACAAATTCTCCGGTCTCTGTAGAGAGTTGTTTAGCTCATTTCATAAAGCCTGAGCTTCTTGCCAATGAAAATGCTTGGCATTGTGAGAACTGTTCCAAATCTCTGAAGCGCCAAAGGTTGGAAGCAAAGAAGCGGCAAAAAGCTGCAACTAAATGTTTGATAAATGGATGTGAGACTAGAGTCCAAAGTGTTTCTCTGAGTTCAGACACTGCTGATATCAGTAATATTAGTAATGGAAATATCAAAAGCAATACTGGTTGCAACCAATTTGGTGAAAACTTGGTTTTGGATGATGGGAAGATGAATTTCTCGAGCGAAAATTGCACATCAATTGAGAATACTCTATCAGATAAGATGATTCCTGTTGTTTGTCAACAACAAGAAGGAAACAGTGGGATGAAAGATGTACTTCCAACAGAATCAAATACTTTGGATTGCAATAACAGTGATACTCTAGAAAGTATTAGCAGTCAAGCCACTGATACATGTGCTGATGTGCCTAGTTGTGCTGGATGTGCCTCTGAAAATGCTCCACAGACAAATTCTAAGTTATTAGATGATTGTGAATTAGAAGCAAGTGAAGATGAGGAAATAAATTCCAAACATGTGAAGGTGAAGAGGGATGCGACAAAACGGGTCCTAATTGACAGGGCCCCGCCTATTTTGACCATTCATCTCAAAAGGTTTAGCCAAGATGCTCGTGGTCGCTTAAGTAAATTGAACGGCCATGTCACTTTCAGAGAAAAAATTGAGCTCAGACCATATATGGATTCCAGGTGCAGAGAAAAGGAGAGGTATGAATACCACCTAATCGGGGTTGTGGAGCATTCTGGGACCATGCGAGGAGGCCATTATGTTGCATATGTGAGAGGTGGTGAAAGGAGCAGAGGAAAAGCTGAGAAGGAGAAGATTGGTCACGTGTGGTATTATGCTAGTGATGCACATGTACGTGAGGTTTCCCTAGATGAGGTGCTTCGCTGTGAGGCCTATATCTTATTCTATGAAAAAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

1005

Amino Acids

109.78

Weight (kDa)

5.52

Isoelectric Point (pI)

48.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	106 - 165	3.8e-13	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	231 - 406	1.9e-25	Ubiquitin carboxyl-terminal hydrolase
UCH	PF00443	706 - 1002	6.7e-31	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04860
fragaria_vesca	FvH4_4g16490 FvH4_4g16490
malus_domestica	MD04G1009400.v1.1 MD13G1284700.v1.1
prunus_persica	Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1
pyrus_communis	pycom04g00720 pycom420g01370
rosa_chinensis	RchiOBHm_Chr4g0415171
rosa_laevigata	RLG00000008090
rosa_multiflora	Rmu_sc0003748.1_g000031 Rmu_sc0033472.1_g000001
rosa_roxburghii	Rroxscaffold_5G00359000
rosa_rugosa	Rorug04G0132500 Rorug04G0132500
rosa_samantha	Rh4AG192800 Rh4BG190900 Rh4CG203600 Rh4DG190700
rosa_wichuraiana	Rw4G016390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	802
Acc36I	ACCTGC	2 cut(s)	802, 1699
AccB1I	GGYRCC	1 cut(s)	242
AccB7I	CCANNNNNTGG	1 cut(s)	2122
AciI	CCGC	6 cut(s)	348, 766, 803, 1314, 2137, 2660
AclWI	GGATC	2 cut(s)	1556, 1979
AcoI	YGGCCR	1 cut(s)	2728
AcsI	RAATTY	6 cut(s)	781, 1725, 2008, 2308, 2545, 2599
AcuI	CTGAAG	4 cut(s)	192, 1515, 1659, 2129
AcvI	CACGTG	1 cut(s)	2923
AfaI	GTAC	8 cut(s)	979, 1006, 1090, 1335, 1362, 1954, 2412, 2952
AflII	CTTAAG	1 cut(s)	2713
AflIII	ACRYGT	4 cut(s)	2489, 2608, 2922, 2947
AhlI	ACTAGT	1 cut(s)	1961
AjnI	CCWGG	3 cut(s)	1166, 1329, 2777
AjuI	GAANNNNNNNTTGG	2 cut(s)	1657, 1689
Alw21I	GWGCWC	4 cut(s)	34, 1323, 1701, 2760
Alw26I	GTCTC	4 cut(s)	1600, 1875, 2023, 2171
AlwI	GGATC	2 cut(s)	1556, 1979
AlwNI	CAGNNNCTG	1 cut(s)	2213
Ama87I	CYCGRG	2 cut(s)	1203, 2314
AoxI	GGCC	6 cut(s)	1919, 1931, 2655, 2728, 2851, 2988
ApaI	GGGCCC	2 cut(s)	1923, 2659
ApeKI	GCWGC	5 cut(s)	480, 931, 1439, 1970, 2147
ApoI	RAATTY	6 cut(s)	781, 1725, 2008, 2308, 2545, 2599
ArsI	GACNNNNNNTTYG	2 cut(s)	1052, 1084
AseI	ATTAAT	2 cut(s)	554, 1575
Asp700I	GAANNNNTTC	1 cut(s)	951
AspLEI	GCGC	1 cut(s)	2115
AspS9I	GGNCC	8 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656, 2838
AsuHPI	GGTGA	9 cut(s)	125, 419, 530, 599, 1328, 1955, 2288, 2629, 2891
AsuNHI	GCTAGC	1 cut(s)	644
AvaI	CYCGRG	2 cut(s)	1203, 2314
AvaII	GGWCC	3 cut(s)	800, 2641, 2838
AxyI	CCTNAGG	2 cut(s)	1182, 1905
BaeGI	GKGCMC	2 cut(s)	1923, 2659
BanI	GGYRCC	1 cut(s)	242
BanII	GRGCYC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
BarI	GAAGNNNNNNTAC	2 cut(s)	997, 1029
BauI	CACGAG	1 cut(s)	2703
BbrPI	CACGTG	1 cut(s)	2923
BbsI	GAAGAC	3 cut(s)	29, 198, 1220
Bbv12I	GWGCWC	4 cut(s)	34, 1323, 1701, 2760
BbvCI	CCTCAGC	1 cut(s)	1686
BbvI	GCAGC	5 cut(s)	492, 943, 1451, 1982, 2134
BceAI	ACGGC	2 cut(s)	1390, 2743
BciT130I	CCWGG	3 cut(s)	1168, 1331, 2779
BcoDI	GTCTC	4 cut(s)	1600, 1875, 2023, 2171
BcuI	ACTAGT	1 cut(s)	1961
BfmI	CTRYAG	2 cut(s)	737, 2022
BfoI	RGCGCY	1 cut(s)	2116
BfrI	CTTAAG	1 cut(s)	2713
BfuAI	ACCTGC	2 cut(s)	802, 1699
BglII	AGATCT	1 cut(s)	1144
BisI	GCNGC	6 cut(s)	481, 932, 1440, 1971, 2138, 2148
BlsI	GCNGC	6 cut(s)	482, 933, 1441, 1972, 2139, 2149
BmcAI	AGTACT	1 cut(s)	1090
Bme1390I	CCNGG	3 cut(s)	1168, 1331, 2779
Bme18I	GGWCC	3 cut(s)	800, 2641, 2838
BmeT110I	CYCGRG	2 cut(s)	1203, 2314
BmgT120I	GGNCC	8 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656, 2838
BmiI	GGNNCC	8 cut(s)	54, 244, 909, 1921, 2642, 2657, 2658, 2839
BmrFI	CCNGG	3 cut(s)	1168, 1331, 2779
BmrI	ACTGGG	3 cut(s)	907, 1812, 1917
BmsI	GCATC	6 cut(s)	1463, 1781, 1841, 2617, 2689, 2932
BmtI	GCTAGC	1 cut(s)	648
BmuI	ACTGGG	3 cut(s)	907, 1812, 1917
BoxI	GACNNNNGTC	1 cut(s)	2183
BpiI	GAAGAC	3 cut(s)	29, 198, 1220
BpmI	CTGGAG	1 cut(s)	1665
Bpu10I	CCTNAGC	3 cut(s)	1686, 1899, 2055
BsaAI	YACGTR	2 cut(s)	2923, 2954
BsaI	GGTCTC	1 cut(s)	2023
BsaJI	CCNNGG	2 cut(s)	903, 1928
BsaWI	WCCGGW	1 cut(s)	2014
BsaXI	ACNNNNNCTCC	6 cut(s)	16, 46, 114, 144, 1996, 2026
Bse21I	CCTNAGG	2 cut(s)	1182, 1905
Bse3DI	GCAATG	1 cut(s)	1703
BseBI	CCWGG	3 cut(s)	1168, 1331, 2779
BseDI	CCNNGG	2 cut(s)	903, 1928
BseMI	GCAATG	1 cut(s)	1703
BseRI	GAGGAG	7 cut(s)	24, 690, 998, 1249, 1703, 1833, 2862
BseSI	GKGCMC	2 cut(s)	1923, 2659
BseXI	GCAGC	5 cut(s)	492, 943, 1451, 1982, 2134
BseYI	CCCAGC	2 cut(s)	1017, 1916
BsgI	GTGCAG	3 cut(s)	461, 499, 2803
BshFI	GGCC	6 cut(s)	1921, 1933, 2657, 2730, 2853, 2990
BshNI	GGYRCC	1 cut(s)	242
BsiHKAI	GWGCWC	4 cut(s)	34, 1323, 1701, 2760
BsiHKCI	CYCGRG	2 cut(s)	1203, 2314
BsiSI	CCGG	2 cut(s)	798, 2015
BslFI	GGGAC	3 cut(s)	1089, 1145, 2851
BsmAI	GTCTC	4 cut(s)	1600, 1875, 2023, 2171
BsmFI	GGGAC	3 cut(s)	1089, 1145, 2851
BsmI	GAATGC	4 cut(s)	350, 1441, 1687, 2830
BsnI	GGCC	6 cut(s)	1921, 1933, 2657, 2730, 2853, 2990
Bso31I	GGTCTC	1 cut(s)	2023
BsoBI	CYCGRG	2 cut(s)	1203, 2314
Bsp120I	GGGCCC	2 cut(s)	1919, 2655
Bsp1286I	GDGCHC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
Bsp143I	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
BspACI	CCGC	6 cut(s)	348, 766, 803, 1314, 2137, 2660
BspANI	GGCC	6 cut(s)	1921, 1933, 2657, 2730, 2853, 2990
BspHI	TCATGA	1 cut(s)	943
BspLI	GGNNCC	8 cut(s)	54, 244, 909, 1921, 2642, 2657, 2658, 2839
BspMI	ACCTGC	2 cut(s)	802, 1699
BspOI	GCTAGC	1 cut(s)	648
BspPI	GGATC	2 cut(s)	1556, 1979
BspT107I	GGYRCC	1 cut(s)	242
BspTI	CTTAAG	1 cut(s)	2713
BspTNI	GGTCTC	1 cut(s)	2023
BsrDI	GCAATG	1 cut(s)	1703
BssECI	CCNNGG	2 cut(s)	903, 1928
BssMI	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
BssSI	CACGAG	1 cut(s)	2703
BssT1I	CCWWGG	2 cut(s)	903, 1928
Bst2BI	CACGAG	1 cut(s)	2703
Bst2UI	CCWGG	3 cut(s)	1168, 1331, 2779
Bst4CI	ACNGT	9 cut(s)	459, 941, 1066, 1093, 1338, 1846, 2096, 2396, 2450
Bst6I	CTCTTC	2 cut(s)	667, 2615
BstAFI	CTTAAG	1 cut(s)	2713
BstBAI	YACGTR	2 cut(s)	2923, 2954
BstC8I	GCNNGC	3 cut(s)	192, 411, 646
BstENI	CCTNNNNNAGG	1 cut(s)	2969
BstH2I	RGCGCY	1 cut(s)	2116
BstHHI	GCGC	1 cut(s)	2115
BstKTI	GATC	7 cut(s)	881, 1147, 1253, 1564, 1620, 1753, 1987
BstMAI	GTCTC	4 cut(s)	1600, 1875, 2023, 2171
BstMBI	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
BstMWI	GCNNNNNNNGC	7 cut(s)	96, 415, 477, 1705, 2325, 2519, 2942
BstNI	CCWGG	3 cut(s)	1168, 1331, 2779
BstNSI	RCATGY	4 cut(s)	413, 2493, 2612, 2951
BstPAI	GACNNNNGTC	1 cut(s)	2183
BstSCI	CCNGG	3 cut(s)	1166, 1329, 2777
BstSFI	CTRYAG	2 cut(s)	737, 2022
BstSLI	GKGCMC	2 cut(s)	1923, 2659
BstV1I	GCAGC	5 cut(s)	492, 943, 1451, 1982, 2134
BstV2I	GAAGAC	3 cut(s)	29, 198, 1220
BstX2I	RGATCY	2 cut(s)	1144, 1561
BstYI	RGATCY	2 cut(s)	1144, 1561
Bsu36I	CCTNAGG	2 cut(s)	1182, 1905
BsuRI	GGCC	6 cut(s)	1921, 1933, 2657, 2730, 2853, 2990
BtgZI	GCGATG	1 cut(s)	478
BtsI	GCAGTG	3 cut(s)	625, 1151, 2211
BveI	ACCTGC	2 cut(s)	802, 1699
Cac8I	GCNNGC	3 cut(s)	192, 411, 646
CaiI	CAGNNNCTG	1 cut(s)	2213
CciI	TCATGA	1 cut(s)	943
CfoI	GCGC	1 cut(s)	2115
Cfr13I	GGNCC	8 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656, 2838
CpoI	CGGWCCG	1 cut(s)	800
CsiI	ACCWGGT	1 cut(s)	1329
Csp6I	GTAC	8 cut(s)	978, 1005, 1089, 1334, 1361, 1953, 2411, 2951
CspCI	CAANNNNNGTGG	2 cut(s)	1219, 1254
CspI	CGGWCCG	1 cut(s)	800
CviQI	GTAC	8 cut(s)	978, 1005, 1089, 1334, 1361, 1953, 2411, 2951
DpnI	GATC	7 cut(s)	880, 1146, 1252, 1563, 1619, 1752, 1986
DpnII	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
EaeI	YGGCCR	1 cut(s)	2728
Eam1104I	CTCTTC	2 cut(s)	667, 2615
EarI	CTCTTC	2 cut(s)	667, 2615
Ecl136II	GAGCTC	4 cut(s)	32, 1321, 1699, 2758
Eco130I	CCWWGG	2 cut(s)	903, 1928
Eco147I	AGGCCT	1 cut(s)	2990
Eco24I	GRGCYC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
Eco31I	GGTCTC	1 cut(s)	2023
Eco32I	GATATC	1 cut(s)	2221
Eco47I	GGWCC	3 cut(s)	800, 2641, 2838
Eco53kI	GAGCTC	4 cut(s)	32, 1321, 1699, 2758
Eco57I	CTGAAG	4 cut(s)	192, 1515, 1659, 2129
Eco72I	CACGTG	1 cut(s)	2923
Eco81I	CCTNAGG	2 cut(s)	1182, 1905
Eco88I	CYCGRG	2 cut(s)	1203, 2314
EcoICRI	GAGCTC	4 cut(s)	32, 1321, 1699, 2758
EcoNI	CCTNNNNNAGG	1 cut(s)	2969
EcoO109I	RGGNCCY	4 cut(s)	1932, 2641, 2655, 2656
EcoRII	CCWGG	3 cut(s)	1166, 1329, 2777
EcoRV	GATATC	1 cut(s)	2221
EcoT14I	CCWWGG	2 cut(s)	903, 1928
EcoT38I	GRGCYC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
ErhI	CCWWGG	2 cut(s)	903, 1928
FalI	AAGNNNNNCTT	4 cut(s)	812, 844, 2397, 2429
FaqI	GGGAC	3 cut(s)	1089, 1145, 2851
FauI	CCCGC	2 cut(s)	759, 2667
FauNDI	CATATG	1 cut(s)	2866
Fnu4HI	GCNGC	6 cut(s)	481, 932, 1440, 1971, 2138, 2148
FriOI	GRGCYC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
Fsp4HI	GCNGC	6 cut(s)	481, 932, 1440, 1971, 2138, 2148
GlaI	GCGC	1 cut(s)	2114
GluI	GCNGC	6 cut(s)	481, 932, 1440, 1971, 2138, 2148
GsaI	CCCAGC	2 cut(s)	1021, 1920
GsuI	CTGGAG	1 cut(s)	1665
HaeII	RGCGCY	1 cut(s)	2116
HaeIII	GGCC	6 cut(s)	1921, 1933, 2657, 2730, 2853, 2990
HapII	CCGG	2 cut(s)	798, 2015
HhaI	GCGC	1 cut(s)	2115
Hin6I	GCGC	1 cut(s)	2113
HinP1I	GCGC	1 cut(s)	2113
HincII	GTYRAC	2 cut(s)	1060, 2378
HindII	GTYRAC	2 cut(s)	1060, 2378
HindIII	AAGCTT	2 cut(s)	147, 1220
HpaII	CCGG	2 cut(s)	798, 2015
HphI	GGTGA	9 cut(s)	125, 419, 530, 599, 1328, 1955, 2288, 2629, 2891
Hpy166II	GTNNAC	5 cut(s)	772, 978, 1060, 1856, 2378
Hpy188III	TCNNGA	8 cut(s)	859, 944, 1203, 1493, 1559, 1988, 2314, 2459
Hpy8I	GTNNAC	5 cut(s)	772, 978, 1060, 1856, 2378
HpyCH4III	ACNGT	9 cut(s)	459, 941, 1066, 1093, 1338, 1846, 2096, 2396, 2450
HpyCH4IV	ACGT	4 cut(s)	1003, 1052, 2922, 2953
HpyF10VI	GCNNNNNNNGC	7 cut(s)	96, 415, 477, 1705, 2325, 2519, 2942
HpySE526I	ACGT	4 cut(s)	1003, 1052, 2922, 2953
HspAI	GCGC	1 cut(s)	2113
Kzo9I	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
LmnI	GCTCC	7 cut(s)	37, 913, 1704, 1799, 2539, 2827, 2886
Lsp1109I	GCAGC	5 cut(s)	492, 943, 1451, 1982, 2134
LweI	GCATC	6 cut(s)	1463, 1781, 1841, 2617, 2689, 2932
MabI	ACCWGGT	1 cut(s)	1329
MaeII	ACGT	4 cut(s)	1003, 1052, 2922, 2953
MaeIII	GTNAC	8 cut(s)	425, 622, 710, 1112, 1149, 1639, 2734, 2918
MalI	GATC	7 cut(s)	880, 1146, 1252, 1563, 1619, 1752, 1986
MboI	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
MfeI	CAATTG	2 cut(s)	1551, 2334
MflI	RGATCY	2 cut(s)	1144, 1561
MhlI	GDGCHC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
MlyI	GAGTC	5 cut(s)	326, 572, 1142, 1988, 2193
MmeI	TCCRAC	3 cut(s)	352, 2103, 2441
MroXI	GAANNNNTTC	1 cut(s)	951
MslI	CAYNNNNRTG	1 cut(s)	2952
MspA1I	CMGCKG	2 cut(s)	1314, 1400
MspCI	CTTAAG	1 cut(s)	2713
MspI	CCGG	2 cut(s)	798, 2015
MspR9I	CCNGG	3 cut(s)	1168, 1331, 2779
MunI	CAATTG	2 cut(s)	1551, 2334
Mva1269I	GAATGC	4 cut(s)	350, 1441, 1687, 2830
MvaI	CCWGG	3 cut(s)	1168, 1331, 2779
MwoI	GCNNNNNNNGC	7 cut(s)	96, 415, 477, 1705, 2325, 2519, 2942
NdeI	CATATG	1 cut(s)	2866
NdeII	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
NheI	GCTAGC	1 cut(s)	644
NlaIV	GGNNCC	8 cut(s)	54, 244, 909, 1921, 2642, 2657, 2658, 2839
NmuCI	GTSAC	6 cut(s)	425, 622, 1112, 1149, 2734, 2918
NspI	RCATGY	4 cut(s)	413, 2493, 2612, 2951
PacI	TTAATTAA	1 cut(s)	558
PaeI	GCATGC	1 cut(s)	413
PaeR7I	CTCGAG	2 cut(s)	1203, 2314
PagI	TCATGA	1 cut(s)	943
PaqCI	CACCTGC	1 cut(s)	802
PceI	AGGCCT	1 cut(s)	2990
PciI	ACATGT	3 cut(s)	2489, 2608, 2947
PctI	GAATGC	4 cut(s)	350, 1441, 1687, 2830
PdmI	GAANNNNTTC	1 cut(s)	951
PfeI	GAWTC	9 cut(s)	62, 305, 855, 1031, 1390, 1409, 2362, 2423, 2774
PflMI	CCANNNNNTGG	1 cut(s)	2122
PkrI	GCNGC	6 cut(s)	482, 933, 1441, 1972, 2139, 2149
PleI	GAGTC	5 cut(s)	325, 572, 1141, 1987, 2192
PmaCI	CACGTG	1 cut(s)	2923
PmlI	CACGTG	1 cut(s)	2923
PpsI	GAGTC	5 cut(s)	325, 572, 1141, 1987, 2192
Ppu21I	YACGTR	2 cut(s)	2923, 2954
PpuMI	RGGWCCY	1 cut(s)	2641
PscI	ACATGT	3 cut(s)	2489, 2608, 2947
PshAI	GACNNNNGTC	1 cut(s)	2183
PshBI	ATTAAT	2 cut(s)	554, 1575
Psp124BI	GAGCTC	4 cut(s)	34, 1323, 1701, 2760
Psp5II	RGGWCCY	1 cut(s)	2641
Psp6I	CCWGG	3 cut(s)	1166, 1329, 2777
PspCI	CACGTG	1 cut(s)	2923
PspFI	CCCAGC	2 cut(s)	1017, 1916
PspGI	CCWGG	3 cut(s)	1166, 1329, 2777
PspN4I	GGNNCC	8 cut(s)	54, 244, 909, 1921, 2642, 2657, 2658, 2839
PspOMI	GGGCCC	2 cut(s)	1919, 2655
PspPI	GGNCC	8 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656, 2838
PspPPI	RGGWCCY	1 cut(s)	2641
PstNI	CAGNNNCTG	1 cut(s)	2213
PsuI	RGATCY	2 cut(s)	1144, 1561
PvuII	CAGCTG	1 cut(s)	1400
RsaI	GTAC	8 cut(s)	979, 1006, 1090, 1335, 1362, 1954, 2412, 2952
RsaNI	GTAC	8 cut(s)	978, 1005, 1089, 1334, 1361, 1953, 2411, 2951
RseI	CAYNNNNRTG	1 cut(s)	2952
Rsr2I	CGGWCCG	1 cut(s)	800
RsrII	CGGWCCG	1 cut(s)	800
SacI	GAGCTC	4 cut(s)	34, 1323, 1701, 2760
SatI	GCNGC	6 cut(s)	481, 932, 1440, 1971, 2138, 2148
Sau3AI	GATC	7 cut(s)	878, 1144, 1250, 1561, 1617, 1750, 1984
Sau96I	GGNCC	8 cut(s)	800, 1919, 1920, 1932, 2641, 2655, 2656, 2838
ScaI	AGTACT	1 cut(s)	1090
SchI	GAGTC	5 cut(s)	326, 572, 1142, 1988, 2193
ScrFI	CCNGG	3 cut(s)	1168, 1331, 2779
SduI	GDGCHC	6 cut(s)	34, 1323, 1701, 1923, 2659, 2760
SexAI	ACCWGGT	1 cut(s)	1329
SfaNI	GCATC	6 cut(s)	1463, 1781, 1841, 2617, 2689, 2932
SfcI	CTRYAG	2 cut(s)	737, 2022
Sfr274I	CTCGAG	2 cut(s)	1203, 2314
SinI	GGWCC	3 cut(s)	800, 2641, 2838
SlaI	CTCGAG	2 cut(s)	1203, 2314
SmiMI	CAYNNNNRTG	1 cut(s)	2952
SmlI	CTYRAG	3 cut(s)	1203, 2314, 2713
SmoI	CTYRAG	3 cut(s)	1203, 2314, 2713
SpeI	ACTAGT	1 cut(s)	1961
SphI	GCATGC	1 cut(s)	413
SseBI	AGGCCT	1 cut(s)	2990
SsiI	CCGC	6 cut(s)	348, 766, 803, 1314, 2137, 2660
SspI	AATATT	1 cut(s)	2230
SstI	GAGCTC	4 cut(s)	34, 1323, 1701, 2760
StuI	AGGCCT	1 cut(s)	2990
StyD4I	CCNGG	3 cut(s)	1166, 1329, 2777
StyI	CCWWGG	2 cut(s)	903, 1928
TaaI	ACNGT	9 cut(s)	459, 941, 1066, 1093, 1338, 1846, 2096, 2396, 2450
TaiI	ACGT	4 cut(s)	1006, 1055, 2925, 2956
TaqI	TCGA	3 cut(s)	1204, 1249, 2315
TatI	WGTACW	3 cut(s)	977, 1088, 2410
TauI	GCSGC	1 cut(s)	2140
TfiI	GAWTC	9 cut(s)	62, 305, 855, 1031, 1390, 1409, 2362, 2423, 2774
TseFI	GTSAC	6 cut(s)	425, 622, 1112, 1149, 2734, 2918
TseI	GCWGC	5 cut(s)	480, 931, 1439, 1970, 2147
Tsp45I	GTSAC	6 cut(s)	425, 622, 1112, 1149, 2734, 2918
TspGWI	ACGGA	1 cut(s)	1515
Van91I	CCANNNNNTGG	1 cut(s)	2122
Vha464I	CTTAAG	1 cut(s)	2713
VpaK11BI	GGWCC	3 cut(s)	800, 2641, 2838
VspI	ATTAAT	2 cut(s)	554, 1575
XagI	CCTNNNNNAGG	1 cut(s)	2969
XapI	RAATTY	6 cut(s)	781, 1725, 2008, 2308, 2545, 2599
XbaI	TCTAGA	1 cut(s)	2458
XceI	RCATGY	4 cut(s)	413, 2493, 2612, 2951
XcmI	CCANNNNNNNNNTGG	2 cut(s)	462, 2702
XhoI	CTCGAG	2 cut(s)	1203, 2314
XmnI	GAANNNNTTC	1 cut(s)	951
ZrmI	AGTACT	1 cut(s)	1090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.