Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008431

Physical Location & Seq

Genomic Coordinates Forward (+)

1086213 .. 1091601

Gene Structure 5389 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1009400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3081 bp

ATGGAATTTATGGGGAAGAAAGTTAAGAAGAAGGCTAGAGCTCCTCCAAGGGAGAAGTTGGTTGCAGCGGATTCCCCCAAAAAAGCCCCCCAACCAAGCAACCCAAGTGTTAAGGACGGTGATGATGGGGTTTCAGTTGCGAAAGAGAGAAGGCCTTGTCCTCATGTTGACAAAGGTGTTGATTTGGATAAATTGTCTGCTAAAATTGGGTCTTCAGGACCTGTTAGGTGTGAAGATTGCAGGGAAGGAGCACTTGATAGGAGGGGTGGTAAAGGAAAGGGAAAACATGGGAAGAAGAAAGGAAGTTCCTCAGTGGATTCAAAATCGGAGTCCAAAGCCATTTGGGTTTGTTTGGAGTGTGGTCATTATTCTTGTGGTGGGATTGGACTTCCTACAACCCCTCAATGTCATGCAGTTCGGCATGCTAGGCAGACTCGTCACCCAATAGTGATCCACTTTGAAAAGCCACAGATGCGGTGGTGCTTCCCGTGCAACATGCTTATTACAATTGAAAAGACGGAAGAGGATGGTGAACAAAAAGATGTGTTTGCTGATGTTGTAAAATTGATAAAGGGTCATTCATCGGAGGGATCATCAGTTAATGCTGAGGATGTGTGGTTTGCGAGTGGCAGTGTGACAAGCGAAATCAAATCAGCTAGCAATATATCAAGTGGTTTGGATGGAAGAGGTGGTTATATGGTTAGAGGCTTGGTTAATCTTGGTAACACTTGCTTCTTTAATTCTGTCTTACAGAACCTTCTAGCCATAGATAGGTTGCGGGGTTACTTCTTAAATTTAGATGCATCTACTGGAGCTCTTACTGTTTCATTGAAGAAACTCTTTACTGAAGCAAAACCAGAGGCAGGACTTAAGAATGTGATAAACCCAAGGTCACTTTTTGGTTGTGTTTGTTCCAAGGCTTCCCAATTTAGGGGGTATCAGCAGCAAGACAGTCATGAATTGCTTCGTTGCTTACTGGATGGATTGTGCACTGAGGAGTTGAGTATGAGGAAACGTATAAATTCTTCTCAGGAAAATGGGAATCCATCAATCTCAGGTCCTACTTTTGTGGAAGCTGCATTTGGGGGCCAAACATCAAGTACTGTTTGCTGTGTTGAATGTGGTCACTCCTCAATTGTGTACGAGTCATTTTTAGATCTGTCATTGTCAGTTCCAACCAGAAAATCCCCATCTAAGGCTGCCCAACCCACCTCTCGAGCAAAGAAGACGAAGTTGCCGCCCAAGAGGAGTGGAAAGCTTCGGCCTAAAAATAACAAAGAGAAGAATTCTGTACCATCACCAAGTGTTCCAACGCCATCAACCAGCGGTGAGGTTTCTGACCATTCTGACCAGCCACAGTCTAGTTCAACCGACCCAAATGTGGCTGAACAAAAGGAATTAGTTGTAAATAGTCTATCTGCTGTCCAAGAATCTCCAAATGAACAAGTCTGTGAGGATGCAGCAGAGCAAACATCAACTTTGTTGGATGACTGTTCAGGGTTGGATTATCTTGATTCGGGAAATATGGTAGATGACAATGAAGTCTGTGAGGATGCAGCAGAGCAAACATCAACTTTGTTGGATGACTGTTCATGGTTGGATTATCTTGATTCGGGAAATATGTTAGATGACAATGATTTCTCTTCACAAAATAATGATGTCTCAATTGTTCAAGATTCCGAAAACAAGAGTTCACATCTTAATGATGTCTCTCTACAGAGTGGCTCTGAATCTAGTAATCAGGTTTTTACACTTAATAAGGAACCGAATGTAGAACCTGACCGTTCTTCAGTGAATTCTTGGGAGGAAGAGCTCCCATTACAGGTTCAGGATTCTGAAGTTTTGTTGCTTCCATATAAAGCAGAGTGTTCTACCACTGAGGAAATCATGGGAGGCGAAGGTGAGGCCTCCTCATCGGTTGTGGGTTGTGGACAAGATGACTTTGGCTTTGGGGACTTATTCAACGAACCTGAAGTTTATGGGCCCCCTACAAGACCCTCTGTAGGTGAGGGAGGCACAGACACTAGTATCATAGCTAGTGAGTCTGATCCTGATGAAGTTGATGATACTGATTCTCCAGTGTCTGTAGAGAGTTGTTTGACTCATTTCATAAGGCCTGAGCTTCTTGCCAATGAAAATGCTTGGCATTGTGAGAACTGTTCCAAAATTCTTCAACGCCAAAGGTTGGAAGGAAAGAAGCAGGGAAAATCTGCTGCTAAAGTTTTGATAAATGGATGTGAGGCTAGAACACAAAGTGATTCACTGAGTTTAAATACGGGTCCTGCTGATGTCAGAAGGCTTGGTAATGGGAATGTCAAAAGCAATACTGGTTGTAATCAGTTTGGTGAAAACTTCGTTTTGCATGATGGAAAAATAAATTGCTGGAACCAAAATTGCTCAGCAATTGAGAATGGTCGATCAGATAAGTCGAATTCTGTTGTTTGTCAACGACAAGATGAGATGGAAGATGCTCCTCCAGTGCAGTCAAATACGTCAGATTGCACTAACGCTTGCAGTCTAGAAAGTTTCAGTGATCAAGTCATTGATTCTAGGGCTGATGAGTCTAGAAGTGCTAGTTTCACCTGTGACATTGTACCACAGACTAATTATGGGATATTGGATGGACATCGTGAATCAGAAGAAAGTGAGGATGAGGAAGTAAATTCCAAACGTGTGAATGTGAAGAGGAATGCGACTAAAAGGTACCTCATCAACAGGGCCCCACCTATTCTGACTATTCATCTTAAGAGGTTCAGCCAAGATGCTCGTGGTCGCTTAAGTAAATTGAATGGCCATATCAGTTTCCAAGAAAAAATTGATCTTAGGCCATATATGGATTCCAGGTGCACAGATGGGGAAAAGTATGAATACCAGCTACTTGGGGTTGTGGAGCATTCTGGAACCATGAGAGGGGGCCACTATGTTGCATACGTGAGAGGGGGTGAAAGGAGCAGAGGAAAAACTGAGAGAGAAAATACTGGTCATGCGTGGTATTATGCTAGCGATGCACATGTGCGTCAGGTTCCCCTAGATGACGTTCTTCGCGCTGAGGCCTATATCTTATTCTATGAAAAAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

1027

Amino Acids

112.14

Weight (kDa)

5.24

Isoelectric Point (pI)

50.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	110 - 169	1.5e-12	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	235 - 1023	7.5e-54	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04860
fragaria_vesca	FvH4_4g16490 FvH4_4g16490
malus_domestica	MD04G1009400.v1.1 MD13G1284700.v1.1
prunus_persica	Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1
pyrus_communis	pycom04g00720 pycom420g01370
rosa_chinensis	RchiOBHm_Chr4g0415171
rosa_laevigata	RLG00000008090
rosa_multiflora	Rmu_sc0003748.1_g000031 Rmu_sc0033472.1_g000001
rosa_roxburghii	Rroxscaffold_5G00359000
rosa_rugosa	Rorug04G0132500 Rorug04G0132500
rosa_samantha	Rh4AG192800 Rh4BG190900 Rh4CG203600 Rh4DG190700
rosa_wichuraiana	Rw4G016390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1541
Acc65I	GGTACC	1 cut(s)	2703
AccB1I	GGYRCC	1 cut(s)	2703
AccB7I	CCANNNNNTGG	1 cut(s)	2185
AccII	CGCG	1 cut(s)	3045
AciI	CCGC	5 cut(s)	68, 475, 778, 1238, 1326
AclWI	GGATC	3 cut(s)	445, 598, 2042
AcoI	YGGCCR	1 cut(s)	2791
AcsI	RAATTY	8 cut(s)	5, 793, 1021, 1285, 1795, 2166, 2431, 2662
AcuI	CTGAAG	5 cut(s)	198, 867, 1773, 1857, 1992
AdeI	CACNNNGTG	2 cut(s)	1304, 2255
AfaI	GTAC	5 cut(s)	1102, 1142, 1293, 2595, 2705
AflII	CTTAAG	3 cut(s)	869, 2744, 2776
AflIII	ACRYGT	2 cut(s)	2671, 3010
AhlI	ACTAGT	1 cut(s)	2024
AjnI	CCWGG	1 cut(s)	2840
AjuI	GAANNNNNNNTTGG	2 cut(s)	1065, 1097
AluBI	AGCT	9 cut(s)	41, 656, 815, 1076, 1258, 1813, 2036, 2122, 2875
AluI	AGCT	9 cut(s)	41, 656, 815, 1076, 1258, 1813, 2036, 2122, 2875
Alw21I	GWGCWC	6 cut(s)	43, 253, 817, 992, 1815, 2849
Alw26I	GTCTC	2 cut(s)	1666, 1714
Alw44I	GTGCAC	2 cut(s)	988, 2845
AlwI	GGATC	3 cut(s)	445, 598, 2042
AlwNI	CAGNNNCTG	2 cut(s)	221, 2084
Ama87I	CYCGRG	1 cut(s)	1215
ApaI	GGGCCC	2 cut(s)	1986, 2722
ApaLI	GTGCAC	2 cut(s)	988, 2845
ApeKI	GCWGC	7 cut(s)	65, 943, 1076, 1199, 1460, 1556, 2213
ApoI	RAATTY	8 cut(s)	5, 793, 1021, 1285, 1795, 2166, 2431, 2662
ArsI	GACNNNNNNTTYG	4 cut(s)	314, 346, 1931, 1963
Asp700I	GAANNNNTTC	1 cut(s)	963
Asp718I	GGTACC	1 cut(s)	2703
AspLEI	GCGC	1 cut(s)	3047
AspS9I	GGNCC	9 cut(s)	218, 1058, 1087, 1982, 1983, 2279, 2718, 2719, 2914
AsuNHI	GCTAGC	2 cut(s)	656, 2999
AvaI	CYCGRG	1 cut(s)	1215
AvaII	GGWCC	3 cut(s)	218, 1058, 2279
BaeGI	GKGCMC	4 cut(s)	992, 1986, 2722, 2849
BaeI	ACNNNNGTAYC	2 cut(s)	2011, 2044
BalI	TGGCCA	1 cut(s)	2793
BanI	GGYRCC	1 cut(s)	2703
BanII	GRGCYC	5 cut(s)	43, 817, 1815, 1986, 2722
BarI	GAAGNNNNNNTAC	2 cut(s)	1009, 1041
BauI	CACGAG	1 cut(s)	2766
BbsI	GAAGAC	2 cut(s)	204, 1232
Bbv12I	GWGCWC	6 cut(s)	43, 253, 817, 992, 1815, 2849
BbvCI	CCTCAGC	2 cut(s)	606, 3048
BbvI	GCAGC	7 cut(s)	77, 955, 1063, 1186, 1472, 1568, 2200
BciT130I	CCWGG	1 cut(s)	2842
BclI	TGATCA	1 cut(s)	2533
BcoDI	GTCTC	2 cut(s)	1666, 1714
BcuI	ACTAGT	1 cut(s)	2024
BfmI	CTRYAG	3 cut(s)	1715, 2001, 2085
BfrI	CTTAAG	3 cut(s)	869, 2744, 2776
BglII	AGATCT	1 cut(s)	1156
BisI	GCNGC	8 cut(s)	66, 944, 1077, 1200, 1238, 1461, 1557, 2214
BlpI	GCTNAGC	1 cut(s)	2398
BlsI	GCNGC	8 cut(s)	67, 945, 1078, 1201, 1239, 1462, 1558, 2215
BmcAI	AGTACT	1 cut(s)	1102
Bme1390I	CCNGG	1 cut(s)	2842
Bme18I	GGWCC	3 cut(s)	218, 1058, 2279
BmeT110I	CYCGRG	1 cut(s)	1215
BmgT120I	GGNCC	9 cut(s)	218, 1058, 1087, 1982, 1983, 2279, 2718, 2719, 2914
BmrFI	CCNGG	1 cut(s)	2842
BmsI	GCATC	8 cut(s)	462, 790, 812, 1447, 1543, 2458, 2752, 2995
BmtI	GCTAGC	2 cut(s)	660, 3003
BpiI	GAAGAC	2 cut(s)	204, 1232
BplI	GAGNNNNNCTC	2 cut(s)	1895, 1927
BpmI	CTGGAG	3 cut(s)	831, 2061, 2460
Bpu10I	CCTNAGC	3 cut(s)	606, 2118, 3048
Bpu1102I	GCTNAGC	1 cut(s)	2398
BsaAI	YACGTR	1 cut(s)	2932
BsaBI	GATNNNNATC	1 cut(s)	2625
BsaJI	CCNNGG	3 cut(s)	47, 887, 915
BsaXI	ACNNNNNCTCC	4 cut(s)	253, 283, 2059, 2089
Bse1I	ACTGG	6 cut(s)	814, 981, 2078, 2332, 2477, 2983
Bse8I	GATNNNNATC	1 cut(s)	2625
BseBI	CCWGG	1 cut(s)	2842
BseDI	CCNNGG	3 cut(s)	47, 887, 915
BseJI	GATNNNNATC	1 cut(s)	2625
BseNI	ACTGG	6 cut(s)	814, 981, 2078, 2332, 2477, 2983
BseRI	GAGGAG	6 cut(s)	33, 1010, 1120, 1261, 1900, 2463
BseSI	GKGCMC	4 cut(s)	992, 1986, 2722, 2849
BseXI	GCAGC	7 cut(s)	77, 955, 1063, 1186, 1472, 1568, 2200
BsgI	GTGCAG	1 cut(s)	2501
Bsh1236I	CGCG	1 cut(s)	3045
BshNI	GGYRCC	1 cut(s)	2703
BsiHKAI	GWGCWC	6 cut(s)	43, 253, 817, 992, 1815, 2849
BsiHKCI	CYCGRG	1 cut(s)	1215
BslFI	GGGAC	1 cut(s)	1967
BsmAI	GTCTC	2 cut(s)	1666, 1714
BsmFI	GGGAC	1 cut(s)	1967
BsmI	GAATGC	2 cut(s)	2695, 2893
BsoBI	CYCGRG	1 cut(s)	1215
Bsp120I	GGGCCC	2 cut(s)	1982, 2718
Bsp1286I	GDGCHC	8 cut(s)	43, 253, 817, 992, 1815, 1986, 2722, 2849
Bsp143I	GATC	7 cut(s)	450, 590, 1156, 2047, 2417, 2533, 2818
Bsp1720I	GCTNAGC	1 cut(s)	2398
BspACI	CCGC	5 cut(s)	68, 475, 778, 1238, 1326
BspFNI	CGCG	1 cut(s)	3045
BspHI	TCATGA	1 cut(s)	955
BspOI	GCTAGC	2 cut(s)	660, 3003
BspPI	GGATC	3 cut(s)	445, 598, 2042
BspQI	GCTCTTC	1 cut(s)	1803
BspT107I	GGYRCC	1 cut(s)	2703
BspTI	CTTAAG	3 cut(s)	869, 2744, 2776
BsrI	ACTGG	6 cut(s)	814, 981, 2078, 2332, 2477, 2983
BssECI	CCNNGG	3 cut(s)	47, 887, 915
BssMI	GATC	7 cut(s)	450, 590, 1156, 2047, 2417, 2533, 2818
BssSI	CACGAG	1 cut(s)	2766
BssT1I	CCWWGG	3 cut(s)	47, 887, 915
Bst2BI	CACGAG	1 cut(s)	2766
Bst2UI	CCWGG	1 cut(s)	2842
Bst4CI	ACNGT	9 cut(s)	119, 823, 953, 1105, 1359, 1493, 1589, 1784, 2159
Bst6I	CTCTTC	5 cut(s)	516, 679, 1648, 1803, 2678
BstAFI	CTTAAG	3 cut(s)	869, 2744, 2776
BstBAI	YACGTR	1 cut(s)	2932
BstC8I	GCNNGC	4 cut(s)	423, 658, 2512, 3001
BstFNI	CGCG	1 cut(s)	3045
BstHHI	GCGC	1 cut(s)	3047
BstKTI	GATC	7 cut(s)	453, 593, 1159, 2050, 2420, 2536, 2821
BstMAI	GTCTC	2 cut(s)	1666, 1714
BstMBI	GATC	7 cut(s)	450, 590, 1156, 2047, 2417, 2533, 2818
BstMWI	GCNNNNNNNGC	4 cut(s)	427, 472, 489, 3005
BstNI	CCWGG	1 cut(s)	2842
BstNSI	RCATGY	3 cut(s)	425, 499, 3014
BstSCI	CCNGG	1 cut(s)	2840
BstSFI	CTRYAG	3 cut(s)	1715, 2001, 2085
BstSLI	GKGCMC	4 cut(s)	992, 1986, 2722, 2849
BstUI	CGCG	1 cut(s)	3045
BstV1I	GCAGC	7 cut(s)	77, 955, 1063, 1186, 1472, 1568, 2200
BstV2I	GAAGAC	2 cut(s)	204, 1232
BstX2I	RGATCY	1 cut(s)	1156
BstXI	CCANNNNNNTGG	1 cut(s)	2879
BstYI	RGATCY	1 cut(s)	1156
BtgZI	GCGATG	1 cut(s)	3018
BtsI	GCAGTG	1 cut(s)	637
BtsIMutI	CAGTG	9 cut(s)	318, 637, 990, 1797, 1875, 2085, 2261, 2484, 2536
Cac8I	GCNNGC	4 cut(s)	423, 658, 2512, 3001
CaiI	CAGNNNCTG	2 cut(s)	221, 2084
CciI	TCATGA	1 cut(s)	955
CfoI	GCGC	1 cut(s)	3047
Cfr13I	GGNCC	9 cut(s)	218, 1058, 1087, 1982, 1983, 2279, 2718, 2719, 2914
CseI	GACGC	1 cut(s)	3005
Csp6I	GTAC	5 cut(s)	1101, 1141, 1292, 2594, 2704
CspCI	CAANNNNNGTGG	2 cut(s)	1231, 1266
CviQI	GTAC	5 cut(s)	1101, 1141, 1292, 2594, 2704
DpnI	GATC	7 cut(s)	452, 592, 1158, 2049, 2419, 2535, 2820
DpnII	GATC	7 cut(s)	450, 590, 1156, 2047, 2417, 2533, 2818
DraI	TTTAAA	1 cut(s)	2271
DraIII	CACNNNGTG	2 cut(s)	1304, 2255
DrdI	GACNNNNNNGTC	1 cut(s)	1541
DseDI	GACNNNNNNGTC	1 cut(s)	1541
EaeI	YGGCCR	1 cut(s)	2791
Eam1104I	CTCTTC	5 cut(s)	516, 679, 1648, 1803, 2678
EarI	CTCTTC	5 cut(s)	516, 679, 1648, 1803, 2678
Ecl136II	GAGCTC	3 cut(s)	41, 815, 1813
Eco130I	CCWWGG	3 cut(s)	47, 887, 915
Eco147I	AGGCCT	4 cut(s)	154, 1907, 2116, 3053
Eco24I	GRGCYC	5 cut(s)	43, 817, 1815, 1986, 2722
Eco47I	GGWCC	3 cut(s)	218, 1058, 2279
Eco53kI	GAGCTC	3 cut(s)	41, 815, 1813
Eco57I	CTGAAG	5 cut(s)	198, 867, 1773, 1857, 1992
Eco88I	CYCGRG	1 cut(s)	1215
EcoICRI	GAGCTC	3 cut(s)	41, 815, 1813
EcoO109I	RGGNCCY	6 cut(s)	218, 1058, 1983, 2279, 2718, 2719
EcoRI	GAATTC	3 cut(s)	1285, 1795, 2431
EcoRII	CCWGG	1 cut(s)	2840
EcoT14I	CCWWGG	3 cut(s)	47, 887, 915
EcoT22I	ATGCAT	1 cut(s)	805
EcoT38I	GRGCYC	5 cut(s)	43, 817, 1815, 1986, 2722
ErhI	CCWWGG	3 cut(s)	47, 887, 915
FalI	AAGNNNNNCTT	4 cut(s)	237, 269, 824, 856
FaqI	GGGAC	1 cut(s)	1967
FauI	CCCGC	1 cut(s)	771
FbaI	TGATCA	1 cut(s)	2533
Fnu4HI	GCNGC	8 cut(s)	66, 944, 1077, 1200, 1238, 1461, 1557, 2214
FriOI	GRGCYC	5 cut(s)	43, 817, 1815, 1986, 2722
Fsp4HI	GCNGC	8 cut(s)	66, 944, 1077, 1200, 1238, 1461, 1557, 2214
GlaI	GCGC	1 cut(s)	3046
GluI	GCNGC	8 cut(s)	66, 944, 1077, 1200, 1238, 1461, 1557, 2214
GsuI	CTGGAG	3 cut(s)	831, 2061, 2460
HgaI	GACGC	1 cut(s)	3005
HhaI	GCGC	1 cut(s)	3047
Hin6I	GCGC	1 cut(s)	3045
HinP1I	GCGC	1 cut(s)	3045
HincII	GTYRAC	2 cut(s)	169, 2447
HindII	GTYRAC	2 cut(s)	169, 2447
HindIII	AAGCTT	1 cut(s)	1256
Hpy166II	GTNNAC	8 cut(s)	169, 533, 990, 1141, 1694, 1931, 2447, 2847
Hpy8I	GTNNAC	8 cut(s)	169, 533, 990, 1141, 1694, 1931, 2447, 2847
HpyAV	CCTTC	7 cut(s)	25, 144, 239, 767, 1892, 2183, 2289
HpyCH4III	ACNGT	9 cut(s)	119, 823, 953, 1105, 1359, 1493, 1589, 1784, 2159
HpyCH4IV	ACGT	5 cut(s)	1015, 2492, 2671, 2931, 3036
HpyF10VI	GCNNNNNNNGC	4 cut(s)	427, 472, 489, 3005
HpySE526I	ACGT	5 cut(s)	1015, 2492, 2671, 2931, 3036
HspAI	GCGC	1 cut(s)	3045
KpnI	GGTACC	1 cut(s)	2707
Ksp22I	TGATCA	1 cut(s)	2533
Kzo9I	GATC	7 cut(s)	450, 590, 1156, 2047, 2417, 2533, 2818
LguI	GCTCTTC	1 cut(s)	1803
LmnI	GCTCC	7 cut(s)	46, 248, 812, 1818, 2476, 2890, 2949
Lsp1109I	GCAGC	7 cut(s)	77, 955, 1063, 1186, 1472, 1568, 2200
LweI	GCATC	8 cut(s)	462, 790, 812, 1447, 1543, 2458, 2752, 2995
MaeII	ACGT	5 cut(s)	1015, 2492, 2671, 2931, 3036
MaeIII	GTNAC	7 cut(s)	437, 634, 722, 782, 891, 1124, 2585
MalI	GATC	7 cut(s)	452, 592, 1158, 2049, 2419, 2535, 2820
MboI	GATC	7 cut(s)	450, 590, 1156, 2047, 2417, 2533, 2818
MfeI	CAATTG	4 cut(s)	507, 1134, 1665, 2403
MflI	RGATCY	1 cut(s)	1156
MhlI	GDGCHC	8 cut(s)	43, 253, 817, 992, 1815, 1986, 2722, 2849
MlsI	TGGCCA	1 cut(s)	2793
MluNI	TGGCCA	1 cut(s)	2793
MlyI	GAGTC	6 cut(s)	338, 427, 1154, 2051, 2095, 2570
MmeI	TCCRAC	7 cut(s)	1199, 1334, 1464, 1482, 1560, 1578, 2166
Mox20I	TGGCCA	1 cut(s)	2793
Mph1103I	ATGCAT	1 cut(s)	805
MroXI	GAANNNNTTC	1 cut(s)	963
MscI	TGGCCA	1 cut(s)	2793
MslI	CAYNNNNRTG	1 cut(s)	1701
Msp20I	TGGCCA	1 cut(s)	2793
MspA1I	CMGCKG	2 cut(s)	68, 1326
MspCI	CTTAAG	3 cut(s)	869, 2744, 2776
MspR9I	CCNGG	1 cut(s)	2842
MunI	CAATTG	4 cut(s)	507, 1134, 1665, 2403
Mva1269I	GAATGC	2 cut(s)	2695, 2893
MvaI	CCWGG	1 cut(s)	2842
MvnI	CGCG	1 cut(s)	3045
MwoI	GCNNNNNNNGC	4 cut(s)	427, 472, 489, 3005
NdeII	GATC	7 cut(s)	450, 590, 1156, 2047, 2417, 2533, 2818
NheI	GCTAGC	2 cut(s)	656, 2999
NmuCI	GTSAC	5 cut(s)	437, 634, 891, 1124, 2585
NsiI	ATGCAT	1 cut(s)	805
NspI	RCATGY	3 cut(s)	425, 499, 3014
PaeI	GCATGC	1 cut(s)	425
PaeR7I	CTCGAG	1 cut(s)	1215
PagI	TCATGA	1 cut(s)	955
PceI	AGGCCT	4 cut(s)	154, 1907, 2116, 3053
PciI	ACATGT	1 cut(s)	3010
PciSI	GCTCTTC	1 cut(s)	1803
PctI	GAATGC	2 cut(s)	2695, 2893
PdmI	GAANNNNTTC	1 cut(s)	963
PflMI	CCANNNNNTGG	1 cut(s)	2185
PkrI	GCNGC	8 cut(s)	67, 945, 1078, 1201, 1239, 1462, 1558, 2215
PleI	GAGTC	6 cut(s)	337, 427, 1153, 2050, 2095, 2569
PpsI	GAGTC	6 cut(s)	337, 427, 1153, 2050, 2095, 2569
Ppu21I	YACGTR	1 cut(s)	2932
PpuMI	RGGWCCY	3 cut(s)	218, 1058, 2279
PscI	ACATGT	1 cut(s)	3010
Psp124BI	GAGCTC	3 cut(s)	43, 817, 1815
Psp5II	RGGWCCY	3 cut(s)	218, 1058, 2279
Psp6I	CCWGG	1 cut(s)	2840
PspGI	CCWGG	1 cut(s)	2840
PspOMI	GGGCCC	2 cut(s)	1982, 2718
PspPI	GGNCC	9 cut(s)	218, 1058, 1087, 1982, 1983, 2279, 2718, 2719, 2914
PspPPI	RGGWCCY	3 cut(s)	218, 1058, 2279
PstNI	CAGNNNCTG	2 cut(s)	221, 2084
PsuI	RGATCY	1 cut(s)	1156
RsaI	GTAC	5 cut(s)	1102, 1142, 1293, 2595, 2705
RsaNI	GTAC	5 cut(s)	1101, 1141, 1292, 2594, 2704
RseI	CAYNNNNRTG	1 cut(s)	1701
SacI	GAGCTC	3 cut(s)	43, 817, 1815
SapI	GCTCTTC	1 cut(s)	1803
SatI	GCNGC	8 cut(s)	66, 944, 1077, 1200, 1238, 1461, 1557, 2214
Sau3AI	GATC	7 cut(s)	450, 590, 1156, 2047, 2417, 2533, 2818
Sau96I	GGNCC	9 cut(s)	218, 1058, 1087, 1982, 1983, 2279, 2718, 2719, 2914
ScaI	AGTACT	1 cut(s)	1102
SchI	GAGTC	6 cut(s)	338, 427, 1154, 2051, 2095, 2570
ScrFI	CCNGG	1 cut(s)	2842
SduI	GDGCHC	8 cut(s)	43, 253, 817, 992, 1815, 1986, 2722, 2849
SfaNI	GCATC	8 cut(s)	462, 790, 812, 1447, 1543, 2458, 2752, 2995
SfcI	CTRYAG	3 cut(s)	1715, 2001, 2085
Sfr274I	CTCGAG	1 cut(s)	1215
SinI	GGWCC	3 cut(s)	218, 1058, 2279
SlaI	CTCGAG	1 cut(s)	1215
SmiMI	CAYNNNNRTG	1 cut(s)	1701
SmlI	CTYRAG	4 cut(s)	869, 1215, 2744, 2776
SmoI	CTYRAG	4 cut(s)	869, 1215, 2744, 2776
SpeI	ACTAGT	1 cut(s)	2024
SphI	GCATGC	1 cut(s)	425
SseBI	AGGCCT	4 cut(s)	154, 1907, 2116, 3053
SsiI	CCGC	5 cut(s)	68, 475, 778, 1238, 1326
SstI	GAGCTC	3 cut(s)	43, 817, 1815
StuI	AGGCCT	4 cut(s)	154, 1907, 2116, 3053
StyD4I	CCNGG	1 cut(s)	2840
StyI	CCWWGG	3 cut(s)	47, 887, 915
TaaI	ACNGT	9 cut(s)	119, 823, 953, 1105, 1359, 1493, 1589, 1784, 2159
TaiI	ACGT	5 cut(s)	1018, 2495, 2674, 2934, 3039
TaqI	TCGA	3 cut(s)	1216, 2416, 2429
TatI	WGTACW	1 cut(s)	1100
TauI	GCSGC	1 cut(s)	1240
TscAI	CASTG	9 cut(s)	318, 637, 997, 1797, 1882, 2085, 2268, 2484, 2536
TseFI	GTSAC	5 cut(s)	437, 634, 891, 1124, 2585
TseI	GCWGC	7 cut(s)	65, 943, 1076, 1199, 1460, 1556, 2213
Tsp45I	GTSAC	5 cut(s)	437, 634, 891, 1124, 2585
TspGWI	ACGGA	1 cut(s)	533
TspRI	CASTG	9 cut(s)	318, 637, 997, 1797, 1882, 2085, 2268, 2484, 2536
Van91I	CCANNNNNTGG	1 cut(s)	2185
Vha464I	CTTAAG	3 cut(s)	869, 2744, 2776
VneI	GTGCAC	2 cut(s)	988, 2845
VpaK11BI	GGWCC	3 cut(s)	218, 1058, 2279
XapI	RAATTY	8 cut(s)	5, 793, 1021, 1285, 1795, 2166, 2431, 2662
XbaI	TCTAGA	2 cut(s)	2518, 2564
XceI	RCATGY	3 cut(s)	425, 499, 3014
XcmI	CCANNNNNNNNNTGG	2 cut(s)	474, 2765
XhoI	CTCGAG	1 cut(s)	1215
XmnI	GAANNNNTTC	1 cut(s)	963
ZrmI	AGTACT	1 cut(s)	1102
Zsp2I	ATGCAT	1 cut(s)	805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.