Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0008431

Physical Location & Seq

Genomic Coordinates Reverse (-)

39051386 .. 39061159

Gene Structure 9774 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00359000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3234 bp

ATGTCGGCCTCCACCGCCGCCACTCGTCCCACCGAGGATGACGCCATCCAACTCCACGATCCCAACCCTCCGGGCTTCGCCGGTTTTGTTCGTCGGATGTCCGGAAGCTCCGAAGCTCCACGCCCGAATCGGGTCCGGGCTTCGCCGGTTTTTTTTGTCGGATGTTCGGGAAGCTCCGAAGCTCCACGCCGGAATCGGGTCCGGGCTCTGAAGCTCATCTCCTTGCGAGATGTCGCAAAAGGCTCCACCTCTAGGGTTGCTCAGAAGCTCTTATTTTCACTTTGGAATCCAAATCTTCAGTTGGGTTTTGTTAAGAAACCTGAACTTATGGGGAAGAAGGTTAAGAAGAAGGCTAGAGCTTCTCAAAAGGAGAAGTGGGTTCCATCTGATTCCCCGAAGAAGGTTGCCGAACCAAGCAACCCAAGCGTTAAGGATGATGGTGATGATGGAGTTTCAGTAGCAACAGTGAGAAAAGCTTGTCCTCACATTGACAAAGGTGTTGATTTGAATAAATTGCAAGCTAAAATTGGGTCTTCAGCAGTTATTAGGTGTGAAGATTGTAGGGAAGGCGCCATTGATAGGAAGGGGGTAAAGGAAAGGGCAAACATGTGCCACGCAGTTCGGCATGCAAGGCAAACTCGTCACCCATTAGTGGTGCAGTTTGAAAACCCACAGTTGCGATGGTGCTTCCAGTGCAGCACACTTATTACCATTGATAAGACGGGGGAGAATGGTGAAGAAAAAGATGTCTTTTCTGATGTTGTGAAATTAATTAAGGGTCATTCGTCGGAGGACTCATCAGGTGATGTTGAGGATGTATGGTTTGGTAGTGGCAGTGTGACAAGCGAAATCAAATCAGCTAGCAATACATCAAGCCCTTTGGATGGAAGAGGAGGTTATGCAGTAAGAGGCTTAGTTAATCTTGGTAACACTTGCTTCTTCAATTCTATTTTACAGAACCTTCTAGCTATTGATAGGTTGCGGGTTTACTTCTTAAATTTTGAAACACCTGCTGGACCGCTTACTATTTCCTTGAAGAAACTCTTTACTGAAACAAAACCAGAGGCAGGATTCAAGAATGTGATAAACCCTAGATCGTTTTTCGGTTGTGTCTGTTCCAAGGCTCCCCAGTTTAGGGGCTATCAGCAGCAAGACAGTCATGAATTGCTTCGTTGCTTACTGGATGGATTGTGTACTGAGGAGTTGAGTATGAGGAAACGTACAAGTTCTTCCCAGCAAAATGAGGATTCATCAAATCCAGTTCCTACATTTGTTGATGCTGTATTTGGGGGACAGGTATCAAGTACTGTTCGCTGTGTTGAATGTGGTCACTCATCAACTATCTATGAGTCATTCTTAGATCTGTCACTGCCAGTCCCAACCAGGAAATCCCCACCTAAGGCTTCCCTACCAAGCTCTCGAGCAAAGAAGACGAAGCTTCCACCCAAGAGGAGTGGAAAGGTTCGATCTAAAATTAACAAAGACAAGAACTCTGCAACATCTCCAAGTGTGGCAACCACATCAACCAGTGGTGAGCTCTCTAACCAGGTACAGTCAAGTTCTACTGAGCTAAATGTACCTGAACAAAACGGCTTGGTTATGAAGAATCCATCAGCTGTCCAAGAATCTCAACATGAACAAGTCTTTGAGAATGCAGCAGGAGAACAAGCATCAGCTTTGTTAGATGACTTTACATGGATGGATTATCTTGATGACGGAAATATATTAGATGACTATGATTTGTCTTCAGAAAATGCTATTGTTTCAATTGTTCAAGATCCTAAAAGTATTAATACATCTCTTAATGATGTCTCACAACAGAGTGGCTCTGAGATCAGTGATAAGGATTTCAAGGTTACTGGAGAACAGAATGTAAAACCAAACTTTTCTTCAGTGAATGCTGAGGAGGACGAGCTCCCAATGCAGGTTCAAAGTTCTGAAATTTTATTGCTTCCATATAAAGAAGATCAGAGTTCTGACATGTTGTTGCTTCCACATAAGGAAGGATGTTCCATCACTGGGGAAATCATGGGAGGAGAAGGAGAGGCATCGTCATCAACTGTGGGTTGTGGACAAGATGATTTTGATGGCTTTGGAGACTTATTCAATGAGCCTGAGCCTGAGGTTGTTGCTGGGCCCAGTCCAAGGCCCTCTACTGGTGAGGAAGGTACATCAACTAGTCTTGTAGCTAGTGAGTCTGATCCTGATGAAGTTGATGATACAAATTCTCCGGTCTCTGTAGAGAGTTGTTTAGCTCATTTCATAAAGCCTGAGCTTCTTGCCAATGAAAATGCTTGGCATTGTGAGAACTGTTCCAAATCTCTGAAGCGCCAAAGGTTGGAAGCAAAGAAGCGGCAAAAAGCTGCAACTAAATGTTTGATAAATGGATGTGAGACTAGAGTCCAAAGTGTTTCTCTGAGTTCAGACACTGCTGAGATCAGTAATATTAGTAATGGAAATATCAAAAGCAATACTGGTTGCAACCAATTTGGTGAAAACTTGGATTTGGATGATGGGAAGATGAATTGCTCGAGCGAAAATTGCACATCAATTGAGAATGCTCTATCAGATAAGATGATTCCTGTTGTTTGTCAACAAGAAGAAGGAAACAGTGAGATGAAAGATGTACTTCCAACAGAATCAAATACTTTGGATTGCAATAACAGTGATACTCTAGAAAGTATTAGCAGTCAAGCCATTGATACATGTGCTGATGTGCCTAGTTGTGCTGGATGTGCCTCTGAAAATGCTCCACAGACAAATTCTAAGTTATTAGATGATTGTGAATTAGAAGCAAGTGAAGATGAGGAAATAAATTCTAAACATGTGAAGGTGAAGAGGGATGCGACAAAACGGGTCCTAATTGACAGGGCCCCGCCTATTTTGACCATTCATCTCAAAAGGTTTAGCCAAGATGCTCGTGGTCGCTTAAGTAAATTGAACGGCCATGTCACTTTCAGAGAAAAATTGAGCTCAGACCATATATGGATTCCAGGTGCAGAGAAAAAGGAGAGGTATGAATACCACCTAATCGGGGTTGTGGAGCATTCTGGGACCATGCGAGGAGGCCATTATGTTGCATATGTGAGAGGTGGTGAAAGGAGCAGAGGAAAAGCTGAGAAGGAGAAGATTGGTCACGTGTGGTATTATGCTAGTGATGCACATGTACGTGAGGTTTCCCTAGATGAGGTGCTTCGCTGTGAGGCCTATATCTTATTCTATGAAAAAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0019538 GO:0019941 GO:0030163 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

1077

Amino Acids

117.77

Weight (kDa)

5.53

Isoelectric Point (pI)

48.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UCH	PF00443	303 - 477	2.4e-25	Ubiquitin carboxyl-terminal hydrolase
UCH	PF00443	778 - 1074	7.4e-30	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04860
fragaria_vesca	FvH4_4g16490 FvH4_4g16490
malus_domestica	MD04G1009400.v1.1 MD13G1284700.v1.1
prunus_persica	Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1 Prupe.1G008700_v2.0.a1
pyrus_communis	pycom04g00720 pycom420g01370
rosa_chinensis	RchiOBHm_Chr4g0415171
rosa_laevigata	RLG00000008090
rosa_multiflora	Rmu_sc0003748.1_g000031 Rmu_sc0033472.1_g000001
rosa_roxburghii	Rroxscaffold_5G00359000
rosa_rugosa	Rorug04G0132500 Rorug04G0132500
rosa_samantha	Rh4AG192800 Rh4BG190900 Rh4CG203600 Rh4DG190700
rosa_wichuraiana	Rw4G016390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1018
Acc36I	ACCTGC	2 cut(s)	1018, 1915
AccB1I	GGYRCC	1 cut(s)	569
AccB7I	CCANNNNNTGG	1 cut(s)	2338
AccIII	TCCGGA	1 cut(s)	101
AciI	CCGC	6 cut(s)	15, 18, 982, 1019, 2353, 2876
AclWI	GGATC	3 cut(s)	53, 1772, 2195
AcoI	YGGCCR	1 cut(s)	2944
AcsI	RAATTY	5 cut(s)	997, 1941, 2224, 2761, 2815
AcuI	CTGAAG	6 cut(s)	230, 281, 519, 1731, 1875, 2345
AcvI	CACGTG	1 cut(s)	3139
AcyI	GRCGYC	2 cut(s)	42, 570
AfaI	GTAC	8 cut(s)	1195, 1222, 1306, 1551, 1578, 2170, 2628, 3168
AflII	CTTAAG	1 cut(s)	2929
AflIII	ACRYGT	6 cut(s)	606, 1980, 2705, 2824, 3138, 3163
AhlI	ACTAGT	1 cut(s)	2177
AjnI	CCWGG	3 cut(s)	1382, 1545, 2992
AjuI	GAANNNNNNNTTGG	2 cut(s)	1873, 1905
Alw21I	GWGCWC	3 cut(s)	1539, 1917, 2975
Alw26I	GTCTC	4 cut(s)	1816, 2091, 2239, 2387
AlwI	GGATC	3 cut(s)	53, 1772, 2195
AlwNI	CAGNNNCTG	1 cut(s)	2429
Ama87I	CYCGRG	2 cut(s)	1419, 2530
Aor13HI	TCCGGA	1 cut(s)	101
AoxI	GGCC	7 cut(s)	6, 2135, 2147, 2871, 2944, 3067, 3204
ApaI	GGGCCC	2 cut(s)	2139, 2875
ApeKI	GCWGC	4 cut(s)	696, 1147, 1655, 2363
ApoI	RAATTY	5 cut(s)	997, 1941, 2224, 2761, 2815
AseI	ATTAAT	2 cut(s)	770, 1791
Asp700I	GAANNNNTTC	1 cut(s)	1167
AspLEI	GCGC	2 cut(s)	572, 2331
AsuC2I	CCSGG	3 cut(s)	72, 137, 203
AsuHPI	GGTGA	9 cut(s)	452, 635, 746, 815, 1544, 2171, 2504, 2845, 3107
AsuNHI	GCTAGC	1 cut(s)	860
AvaI	CYCGRG	2 cut(s)	1419, 2530
AvaII	GGWCC	5 cut(s)	133, 199, 1016, 2857, 3054
AxyI	CCTNAGG	2 cut(s)	1398, 2121
BaeGI	GKGCMC	2 cut(s)	2139, 2875
BanI	GGYRCC	1 cut(s)	569
BanII	GRGCYC	6 cut(s)	208, 1539, 1917, 2139, 2875, 2975
BarI	GAAGNNNNNNTAC	2 cut(s)	1213, 1245
BauI	CACGAG	1 cut(s)	2919
BbrPI	CACGTG	1 cut(s)	3139
BbsI	GAAGAC	3 cut(s)	525, 1436, 1737
Bbv12I	GWGCWC	3 cut(s)	1539, 1917, 2975
BbvCI	CCTCAGC	1 cut(s)	1902
BbvI	GCAGC	4 cut(s)	708, 1159, 1667, 2350
BceAI	ACGGC	2 cut(s)	1606, 2959
BciT130I	CCWGG	3 cut(s)	1384, 1547, 2994
BcnI	CCSGG	3 cut(s)	72, 137, 203
BcoDI	GTCTC	4 cut(s)	1816, 2091, 2239, 2387
BcuI	ACTAGT	1 cut(s)	2177
BfmI	CTRYAG	1 cut(s)	2238
BfoI	RGCGCY	2 cut(s)	573, 2332
BfrI	CTTAAG	1 cut(s)	2929
BfuAI	ACCTGC	2 cut(s)	1018, 1915
BglII	AGATCT	1 cut(s)	1360
BisI	GCNGC	6 cut(s)	18, 697, 1148, 1656, 2354, 2364
BlsI	GCNGC	6 cut(s)	19, 698, 1149, 1657, 2355, 2365
BmcAI	AGTACT	1 cut(s)	1306
Bme1390I	CCNGG	6 cut(s)	72, 137, 203, 1384, 1547, 2994
Bme18I	GGWCC	5 cut(s)	133, 199, 1016, 2857, 3054
BmeT110I	CYCGRG	2 cut(s)	1419, 2530
BmrFI	CCNGG	6 cut(s)	72, 137, 203, 1384, 1547, 2994
BmrI	ACTGGG	3 cut(s)	1123, 2028, 2133
BmsI	GCATC	6 cut(s)	1267, 1679, 2057, 2833, 2905, 3148
BmtI	GCTAGC	1 cut(s)	864
BmuI	ACTGGG	3 cut(s)	1123, 2028, 2133
BoxI	GACNNNNGTC	1 cut(s)	2399
BpiI	GAAGAC	3 cut(s)	525, 1436, 1737
BpmI	CTGGAG	1 cut(s)	1881
Bpu10I	CCTNAGC	3 cut(s)	1902, 2115, 2271
BpuMI	CCSGG	3 cut(s)	72, 137, 203
BsaAI	YACGTR	2 cut(s)	3139, 3170
BsaHI	GRCGYC	2 cut(s)	42, 570
BsaI	GGTCTC	1 cut(s)	2239
BsaJI	CCNNGG	3 cut(s)	33, 1119, 2144
BsaWI	WCCGGW	2 cut(s)	101, 2230
BsaXI	ACNNNNNCTCC	4 cut(s)	441, 471, 2212, 2242
Bse118I	RCCGGY	2 cut(s)	80, 145
Bse21I	CCTNAGG	2 cut(s)	1398, 2121
BseAI	TCCGGA	1 cut(s)	101
BseBI	CCWGG	3 cut(s)	1384, 1547, 2994
BseDI	CCNNGG	3 cut(s)	33, 1119, 2144
BseRI	GAGGAG	6 cut(s)	906, 1214, 1465, 1919, 2049, 3078
BseSI	GKGCMC	2 cut(s)	2139, 2875
BseXI	GCAGC	4 cut(s)	708, 1159, 1667, 2350
BseYI	CCCAGC	2 cut(s)	1233, 2132
BsgI	GTGCAG	3 cut(s)	677, 715, 3018
BshFI	GGCC	7 cut(s)	8, 2137, 2149, 2873, 2946, 3069, 3206
BshNI	GGYRCC	1 cut(s)	569
BsiHKAI	GWGCWC	3 cut(s)	1539, 1917, 2975
BsiHKCI	CYCGRG	2 cut(s)	1419, 2530
BsiSI	CCGG	8 cut(s)	71, 81, 102, 136, 146, 190, 202, 2231
BslFI	GGGAC	4 cut(s)	12, 1305, 1361, 3067
BsmAI	GTCTC	4 cut(s)	1816, 2091, 2239, 2387
BsmFI	GGGAC	4 cut(s)	12, 1305, 1361, 3067
BsmI	GAATGC	4 cut(s)	1657, 1903, 2563, 3046
BsnI	GGCC	7 cut(s)	8, 2137, 2149, 2873, 2946, 3069, 3206
Bso31I	GGTCTC	1 cut(s)	2239
BsoBI	CYCGRG	2 cut(s)	1419, 2530
Bsp120I	GGGCCC	2 cut(s)	2135, 2871
Bsp1286I	GDGCHC	6 cut(s)	208, 1539, 1917, 2139, 2875, 2975
Bsp13I	TCCGGA	1 cut(s)	101
Bsp143I	GATC	9 cut(s)	58, 1094, 1360, 1466, 1777, 1833, 1966, 2200, 2436
BspACI	CCGC	6 cut(s)	15, 18, 982, 1019, 2353, 2876
BspANI	GGCC	7 cut(s)	8, 2137, 2149, 2873, 2946, 3069, 3206
BspEI	TCCGGA	1 cut(s)	101
BspHI	TCATGA	1 cut(s)	1159
BspMI	ACCTGC	2 cut(s)	1018, 1915
BspOI	GCTAGC	1 cut(s)	864
BspPI	GGATC	3 cut(s)	53, 1772, 2195
BspT107I	GGYRCC	1 cut(s)	569
BspTI	CTTAAG	1 cut(s)	2929
BspTNI	GGTCTC	1 cut(s)	2239
BsrFI	RCCGGY	2 cut(s)	80, 145
BssAI	RCCGGY	2 cut(s)	80, 145
BssECI	CCNNGG	3 cut(s)	33, 1119, 2144
BssMI	GATC	9 cut(s)	58, 1094, 1360, 1466, 1777, 1833, 1966, 2200, 2436
BssNI	GRCGYC	2 cut(s)	42, 570
BssSI	CACGAG	1 cut(s)	2919
BssT1I	CCWWGG	2 cut(s)	1119, 2144
Bst2BI	CACGAG	1 cut(s)	2919
Bst2UI	CCWGG	3 cut(s)	1384, 1547, 2994
Bst4CI	ACNGT	9 cut(s)	466, 675, 1157, 1309, 1554, 2062, 2312, 2612, 2666
Bst6I	CTCTTC	2 cut(s)	883, 2831
BstACI	GRCGYC	2 cut(s)	42, 570
BstAFI	CTTAAG	1 cut(s)	2929
BstBAI	YACGTR	2 cut(s)	3139, 3170
BstC8I	GCNNGC	3 cut(s)	519, 627, 862
BstENI	CCTNNNNNAGG	1 cut(s)	3185
BstH2I	RGCGCY	2 cut(s)	573, 2332
BstHHI	GCGC	2 cut(s)	572, 2331
BstKTI	GATC	9 cut(s)	61, 1097, 1363, 1469, 1780, 1836, 1969, 2203, 2439
BstMAI	GTCTC	4 cut(s)	1816, 2091, 2239, 2387
BstMBI	GATC	9 cut(s)	58, 1094, 1360, 1466, 1777, 1833, 1966, 2200, 2436
BstMWI	GCNNNNNNNGC	8 cut(s)	14, 423, 631, 693, 1921, 2541, 2735, 3158
BstNI	CCWGG	3 cut(s)	1384, 1547, 2994
BstNSI	RCATGY	6 cut(s)	610, 629, 1984, 2709, 2828, 3167
BstPAI	GACNNNNGTC	1 cut(s)	2399
BstSCI	CCNGG	6 cut(s)	70, 135, 201, 1382, 1545, 2992
BstSFI	CTRYAG	1 cut(s)	2238
BstSLI	GKGCMC	2 cut(s)	2139, 2875
BstV1I	GCAGC	4 cut(s)	708, 1159, 1667, 2350
BstV2I	GAAGAC	3 cut(s)	525, 1436, 1737
BstX2I	RGATCY	2 cut(s)	1360, 1777
BstYI	RGATCY	2 cut(s)	1360, 1777
Bsu36I	CCTNAGG	2 cut(s)	1398, 2121
BsuRI	GGCC	7 cut(s)	8, 2137, 2149, 2873, 2946, 3069, 3206
BtgZI	GCGATG	1 cut(s)	694
BtsI	GCAGTG	3 cut(s)	841, 1367, 2427
BveI	ACCTGC	2 cut(s)	1018, 1915
Cac8I	GCNNGC	3 cut(s)	519, 627, 862
CaiI	CAGNNNCTG	1 cut(s)	2429
CciI	TCATGA	1 cut(s)	1159
CfoI	GCGC	2 cut(s)	572, 2331
Cfr10I	RCCGGY	2 cut(s)	80, 145
CseI	GACGC	1 cut(s)	50
CsiI	ACCWGGT	1 cut(s)	1545
Csp6I	GTAC	8 cut(s)	1194, 1221, 1305, 1550, 1577, 2169, 2627, 3167
CspCI	CAANNNNNGTGG	2 cut(s)	1435, 1470
CviQI	GTAC	8 cut(s)	1194, 1221, 1305, 1550, 1577, 2169, 2627, 3167
DinI	GGCGCC	1 cut(s)	571
DpnI	GATC	9 cut(s)	60, 1096, 1362, 1468, 1779, 1835, 1968, 2202, 2438
DpnII	GATC	9 cut(s)	58, 1094, 1360, 1466, 1777, 1833, 1966, 2200, 2436
EaeI	YGGCCR	1 cut(s)	2944
Eam1104I	CTCTTC	2 cut(s)	883, 2831
EarI	CTCTTC	2 cut(s)	883, 2831
Ecl136II	GAGCTC	3 cut(s)	1537, 1915, 2973
Eco130I	CCWWGG	2 cut(s)	1119, 2144
Eco147I	AGGCCT	1 cut(s)	3206
Eco24I	GRGCYC	6 cut(s)	208, 1539, 1917, 2139, 2875, 2975
Eco31I	GGTCTC	1 cut(s)	2239
Eco47I	GGWCC	5 cut(s)	133, 199, 1016, 2857, 3054
Eco53kI	GAGCTC	3 cut(s)	1537, 1915, 2973
Eco57I	CTGAAG	6 cut(s)	230, 281, 519, 1731, 1875, 2345
Eco72I	CACGTG	1 cut(s)	3139
Eco81I	CCTNAGG	2 cut(s)	1398, 2121
Eco88I	CYCGRG	2 cut(s)	1419, 2530
EcoICRI	GAGCTC	3 cut(s)	1537, 1915, 2973
EcoNI	CCTNNNNNAGG	1 cut(s)	3185
EcoO109I	RGGNCCY	4 cut(s)	2148, 2857, 2871, 2872
EcoRII	CCWGG	3 cut(s)	1382, 1545, 2992
EcoT14I	CCWWGG	2 cut(s)	1119, 2144
EcoT38I	GRGCYC	6 cut(s)	208, 1539, 1917, 2139, 2875, 2975
EgeI	GGCGCC	1 cut(s)	571
EheI	GGCGCC	1 cut(s)	571
ErhI	CCWWGG	2 cut(s)	1119, 2144
FalI	AAGNNNNNCTT	4 cut(s)	1028, 1060, 2613, 2645
FaqI	GGGAC	4 cut(s)	12, 1305, 1361, 3067
FauI	CCCGC	2 cut(s)	975, 2883
FauNDI	CATATG	1 cut(s)	3082
Fnu4HI	GCNGC	6 cut(s)	18, 697, 1148, 1656, 2354, 2364
FriOI	GRGCYC	6 cut(s)	208, 1539, 1917, 2139, 2875, 2975
Fsp4HI	GCNGC	6 cut(s)	18, 697, 1148, 1656, 2354, 2364
GlaI	GCGC	2 cut(s)	571, 2330
GluI	GCNGC	6 cut(s)	18, 697, 1148, 1656, 2354, 2364
GsaI	CCCAGC	2 cut(s)	1237, 2136
GsuI	CTGGAG	1 cut(s)	1881
HaeII	RGCGCY	2 cut(s)	573, 2332
HaeIII	GGCC	7 cut(s)	8, 2137, 2149, 2873, 2946, 3069, 3206
HapII	CCGG	8 cut(s)	71, 81, 102, 136, 146, 190, 202, 2231
HgaI	GACGC	1 cut(s)	50
HhaI	GCGC	2 cut(s)	572, 2331
Hin1I	GRCGYC	2 cut(s)	42, 570
Hin6I	GCGC	2 cut(s)	570, 2329
HinP1I	GCGC	2 cut(s)	570, 2329
HincII	GTYRAC	1 cut(s)	2594
HindII	GTYRAC	1 cut(s)	2594
HindIII	AAGCTT	2 cut(s)	474, 1436
HpaII	CCGG	8 cut(s)	71, 81, 102, 136, 146, 190, 202, 2231
HphI	GGTGA	9 cut(s)	452, 635, 746, 815, 1544, 2171, 2504, 2845, 3107
Hpy166II	GTNNAC	4 cut(s)	988, 1194, 2072, 2594
Hpy188III	TCNNGA	9 cut(s)	102, 168, 1075, 1160, 1419, 1709, 1775, 2204, 2675
Hpy8I	GTNNAC	4 cut(s)	988, 1194, 2072, 2594
Hpy99I	CGWCG	2 cut(s)	96, 790
HpyCH4III	ACNGT	9 cut(s)	466, 675, 1157, 1309, 1554, 2062, 2312, 2612, 2666
HpyCH4IV	ACGT	3 cut(s)	1219, 3138, 3169
HpyF10VI	GCNNNNNNNGC	8 cut(s)	14, 423, 631, 693, 1921, 2541, 2735, 3158
HpySE526I	ACGT	3 cut(s)	1219, 3138, 3169
Hsp92I	GRCGYC	2 cut(s)	42, 570
HspAI	GCGC	2 cut(s)	570, 2329
KasI	GGCGCC	1 cut(s)	569
Kpn2I	TCCGGA	1 cut(s)	101
Kzo9I	GATC	9 cut(s)	58, 1094, 1360, 1466, 1777, 1833, 1966, 2200, 2436
Lsp1109I	GCAGC	4 cut(s)	708, 1159, 1667, 2350
LweI	GCATC	6 cut(s)	1267, 1679, 2057, 2833, 2905, 3148
MabI	ACCWGGT	1 cut(s)	1545
MaeII	ACGT	3 cut(s)	1219, 3138, 3169
MaeIII	GTNAC	8 cut(s)	641, 838, 926, 1328, 1365, 1855, 2950, 3134
MalI	GATC	9 cut(s)	60, 1096, 1362, 1468, 1779, 1835, 1968, 2202, 2438
MboI	GATC	9 cut(s)	58, 1094, 1360, 1466, 1777, 1833, 1966, 2200, 2436
MfeI	CAATTG	2 cut(s)	1767, 2550
MflI	RGATCY	2 cut(s)	1360, 1777
MhlI	GDGCHC	6 cut(s)	208, 1539, 1917, 2139, 2875, 2975
Mly113I	GGCGCC	1 cut(s)	570
MlyI	GAGTC	4 cut(s)	788, 1358, 2204, 2409
MmeI	TCCRAC	6 cut(s)	73, 74, 139, 768, 2319, 2657
MroI	TCCGGA	1 cut(s)	101
MroXI	GAANNNNTTC	1 cut(s)	1167
MslI	CAYNNNNRTG	1 cut(s)	3168
MspA1I	CMGCKG	1 cut(s)	1616
MspCI	CTTAAG	1 cut(s)	2929
MspI	CCGG	8 cut(s)	71, 81, 102, 136, 146, 190, 202, 2231
MspR9I	CCNGG	6 cut(s)	72, 137, 203, 1384, 1547, 2994
MunI	CAATTG	2 cut(s)	1767, 2550
Mva1269I	GAATGC	4 cut(s)	1657, 1903, 2563, 3046
MvaI	CCWGG	3 cut(s)	1384, 1547, 2994
MwoI	GCNNNNNNNGC	8 cut(s)	14, 423, 631, 693, 1921, 2541, 2735, 3158
NarI	GGCGCC	1 cut(s)	570
NciI	CCSGG	3 cut(s)	72, 137, 203
NdeI	CATATG	1 cut(s)	3082
NdeII	GATC	9 cut(s)	58, 1094, 1360, 1466, 1777, 1833, 1966, 2200, 2436
NheI	GCTAGC	1 cut(s)	860
NmuCI	GTSAC	6 cut(s)	641, 838, 1328, 1365, 2950, 3134
NspI	RCATGY	6 cut(s)	610, 629, 1984, 2709, 2828, 3167
PacI	TTAATTAA	1 cut(s)	774
PaeI	GCATGC	1 cut(s)	629
PaeR7I	CTCGAG	2 cut(s)	1419, 2530
PagI	TCATGA	1 cut(s)	1159
PaqCI	CACCTGC	1 cut(s)	1018
PceI	AGGCCT	1 cut(s)	3206
PciI	ACATGT	5 cut(s)	606, 1980, 2705, 2824, 3163
PctI	GAATGC	4 cut(s)	1657, 1903, 2563, 3046
PdmI	GAANNNNTTC	1 cut(s)	1167
PflMI	CCANNNNNTGG	1 cut(s)	2338
PkrI	GCNGC	6 cut(s)	19, 698, 1149, 1657, 2355, 2365
PleI	GAGTC	4 cut(s)	788, 1357, 2203, 2408
PluTI	GGCGCC	1 cut(s)	573
PmaCI	CACGTG	1 cut(s)	3139
PmlI	CACGTG	1 cut(s)	3139
PpsI	GAGTC	4 cut(s)	788, 1357, 2203, 2408
Ppu21I	YACGTR	2 cut(s)	3139, 3170
PpuMI	RGGWCCY	1 cut(s)	2857
PscI	ACATGT	5 cut(s)	606, 1980, 2705, 2824, 3163
PshAI	GACNNNNGTC	1 cut(s)	2399
PshBI	ATTAAT	2 cut(s)	770, 1791
Psp124BI	GAGCTC	3 cut(s)	1539, 1917, 2975
Psp5II	RGGWCCY	1 cut(s)	2857
Psp6I	CCWGG	3 cut(s)	1382, 1545, 2992
PspCI	CACGTG	1 cut(s)	3139
PspFI	CCCAGC	2 cut(s)	1233, 2132
PspGI	CCWGG	3 cut(s)	1382, 1545, 2992
PspOMI	GGGCCC	2 cut(s)	2135, 2871
PspPPI	RGGWCCY	1 cut(s)	2857
PspXI	VCTCGAGB	1 cut(s)	2530
PstNI	CAGNNNCTG	1 cut(s)	2429
PsuI	RGATCY	2 cut(s)	1360, 1777
PvuII	CAGCTG	1 cut(s)	1616
RsaI	GTAC	8 cut(s)	1195, 1222, 1306, 1551, 1578, 2170, 2628, 3168
RsaNI	GTAC	8 cut(s)	1194, 1221, 1305, 1550, 1577, 2169, 2627, 3167
RseI	CAYNNNNRTG	1 cut(s)	3168
SacI	GAGCTC	3 cut(s)	1539, 1917, 2975
SatI	GCNGC	6 cut(s)	18, 697, 1148, 1656, 2354, 2364
Sau3AI	GATC	9 cut(s)	58, 1094, 1360, 1466, 1777, 1833, 1966, 2200, 2436
ScaI	AGTACT	1 cut(s)	1306
SchI	GAGTC	4 cut(s)	788, 1358, 2204, 2409
ScrFI	CCNGG	6 cut(s)	72, 137, 203, 1384, 1547, 2994
SduI	GDGCHC	6 cut(s)	208, 1539, 1917, 2139, 2875, 2975
SexAI	ACCWGGT	1 cut(s)	1545
SfaNI	GCATC	6 cut(s)	1267, 1679, 2057, 2833, 2905, 3148
SfcI	CTRYAG	1 cut(s)	2238
SfoI	GGCGCC	1 cut(s)	571
Sfr274I	CTCGAG	2 cut(s)	1419, 2530
SinI	GGWCC	5 cut(s)	133, 199, 1016, 2857, 3054
SlaI	CTCGAG	2 cut(s)	1419, 2530
SmiMI	CAYNNNNRTG	1 cut(s)	3168
SmlI	CTYRAG	3 cut(s)	1419, 2530, 2929
SmoI	CTYRAG	3 cut(s)	1419, 2530, 2929
SpeI	ACTAGT	1 cut(s)	2177
SphI	GCATGC	1 cut(s)	629
SseBI	AGGCCT	1 cut(s)	3206
SsiI	CCGC	6 cut(s)	15, 18, 982, 1019, 2353, 2876
SspDI	GGCGCC	1 cut(s)	569
SspI	AATATT	1 cut(s)	2446
SstI	GAGCTC	3 cut(s)	1539, 1917, 2975
StuI	AGGCCT	1 cut(s)	3206
StyD4I	CCNGG	6 cut(s)	70, 135, 201, 1382, 1545, 2992
StyI	CCWWGG	2 cut(s)	1119, 2144
TaaI	ACNGT	9 cut(s)	466, 675, 1157, 1309, 1554, 2062, 2312, 2612, 2666
TaiI	ACGT	3 cut(s)	1222, 3141, 3172
TaqI	TCGA	3 cut(s)	1420, 1465, 2531
TatI	WGTACW	3 cut(s)	1193, 1304, 2626
TauI	GCSGC	2 cut(s)	20, 2356
TseFI	GTSAC	6 cut(s)	641, 838, 1328, 1365, 2950, 3134
TseI	GCWGC	4 cut(s)	696, 1147, 1655, 2363
Tsp45I	GTSAC	6 cut(s)	641, 838, 1328, 1365, 2950, 3134
TspGWI	ACGGA	1 cut(s)	1731
Van91I	CCANNNNNTGG	1 cut(s)	2338
Vha464I	CTTAAG	1 cut(s)	2929
VpaK11BI	GGWCC	5 cut(s)	133, 199, 1016, 2857, 3054
VspI	ATTAAT	2 cut(s)	770, 1791
XagI	CCTNNNNNAGG	1 cut(s)	3185
XapI	RAATTY	5 cut(s)	997, 1941, 2224, 2761, 2815
XbaI	TCTAGA	1 cut(s)	2674
XceI	RCATGY	6 cut(s)	610, 629, 1984, 2709, 2828, 3167
XcmI	CCANNNNNNNNNTGG	2 cut(s)	678, 2918
XhoI	CTCGAG	2 cut(s)	1419, 2530
XmnI	GAANNNNTTC	1 cut(s)	1167
ZrmI	AGTACT	1 cut(s)	1306

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.