AT1G04890

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005611
Physical Location & Seq
Reverse (-)
1380382 .. 1382599
2218 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04890.1

Sequence Viewer

Length: 1236 bp
ATGACCGTTCAGGGCATGAAAGGCAAACGTTTTGTTACAAGAAGATCAAGAAACGGTCTTAAAAACCATAGGAGATCAGGTCGTGTAAACTGTGCAAGTCTCAGATCTATTTCTTCATCAGAAGCCTTTGATGAGACCGCAAAAACCAATTTGATGCAGCCTTATGATGAGACATGTTCCAATGATGACCAAAAAGATTGTGGTAAGGCAGGAGAGGGGACTAATCTATCTCATTGGCCGGAATTTGAGAAAACCGTATCAGTTAATCAACAGGTAAACTCCAATTCATCAGCAGAACAATCTTTTGTGAGAAATGTAGAGAAAAGATCTGTTAGGGATTTAGAAGAATTATTGAAAGAAGAGAGAGCGGCTCGAGCAACGGTTTGTGTCGAGCTTGACAAAGAGAGAAGCGCTGCAGCATCAGCAGCGGATGAAGCGATGGCGATGATACATAGGCTGCAAGATGAGAAGGCGGCAATTGAAATGGAAGCGAGGCAGTTCCAGAGACTGGTTGAAGAGAGATCGACTTTTGATGCTGAAGAGATGGTCATACTCAAAGACATTTTGATACGGCGTGAGAGAGAAAAACATTTCTTGGAGAAAGAAGTTGAAGCTTATAGACAACTACTCGAAGAAACAGAGGAATTGGAATGTTCTTTAATAAAAGAGAAGAATGTTCCAGAACCGGAACACAAACAAAACAAAGACTGTCAAGAGAGAAGAGCTTTGCTCGTTCAGGAACTTGATGGAACGGTTCTCGATATGCCTTATAGAGAAGAAGGAAACAGAGATAAAAACAGAGATCTGTATAAATCAGATTCAGAAGTCGCGTACTCACGTGTGCGTGATGTTTATATGGTGAAGGACGAAACAGAGAACATTAGTAAGAAGAAGAATCTAGAAGAATCCTCTGTTGGCAAACCTAAAGAATCGTTGGATGAAAACAGTATCATTGTCTCGGGGATTGCTCGAAAGCTACCTCCCTTATGTCGTCCACGCAAGAAGTCGCTGAGCTCTTCGGGTTCAAGGAGAAAATCGATGTCTGCGGTTGATTATGAGAGGTTAAAGATTGAAAATGAAGTGGAATTGTTAAGAGAAAGATTAAAGGCTGTTCAAGAGGAAAGAGAGGAGCTTACGAGACGAGCATCTTTGCCTCCTTTACCATCAAAGGTTAGGGCTACGTCAGAGAAGCGAAGTTGGACAAGATCTTGTTCCATGGATCTTCACTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

411

Amino Acids

47.36

Weight (kDa)

6.68

Isoelectric Point (pI)

61.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 113 - 203 2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 508
AccBSI CCGCTC 1 cut(s) 368
AccII CGCG 1 cut(s) 830
AciI CCGC 5 cut(s) 138, 368, 428, 473, 1046
AclI AACGTT 1 cut(s) 28
AclWI GGATC 1 cut(s) 1227
AcoI YGGCCR 1 cut(s) 236
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 558
AcvI CACGTG 1 cut(s) 839
AfaI GTAC 1 cut(s) 833
AfeI AGCGCT 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 508
AflIII ACRYGT 2 cut(s) 173, 838
AgsI TTSAA 7 cut(s) 355, 482, 515, 611, 1026, 1073, 1115
AjuI GAANNNNNNNTTGG 2 cut(s) 897, 929
AluBI AGCT 6 cut(s) 394, 614, 725, 976, 1014, 1132
AluI AGCT 6 cut(s) 394, 614, 725, 976, 1014, 1132
Alw21I GWGCWC 1 cut(s) 1016
Alw26I GTCTC 6 cut(s) 104, 128, 164, 499, 961, 1132
AlwI GGATC 1 cut(s) 1227
AlwNI CAGNNNCTG 1 cut(s) 508
Ama87I CYCGRG 2 cut(s) 372, 958
Aor51HI AGCGCT 1 cut(s) 412
AoxI GGCC 1 cut(s) 236
ApeKI GCWGC 5 cut(s) 157, 413, 416, 425, 457
ApoI RAATTY 1 cut(s) 242
AspLEI GCGC 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 871
AvaI CYCGRG 2 cut(s) 372, 958
BanII GRGCYC 1 cut(s) 1016
BbrPI CACGTG 1 cut(s) 839
Bbv12I GWGCWC 1 cut(s) 1016
BbvI GCAGC 5 cut(s) 169, 400, 428, 437, 444
BccI CCATC 4 cut(s) 433, 538, 740, 1171
BceAI ACGGC 1 cut(s) 587
BcoDI GTCTC 6 cut(s) 104, 128, 164, 499, 961, 1132
BfaI CTAG 1 cut(s) 899
BfmI CTRYAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 414
BglII AGATCT 4 cut(s) 104, 326, 802, 1205
BisI GCNGC 7 cut(s) 158, 369, 414, 417, 426, 458, 474
BlpI GCTNAGC 1 cut(s) 1010
BlsI GCNGC 7 cut(s) 159, 370, 415, 418, 427, 459, 475
BmeT110I CYCGRG 2 cut(s) 372, 958
BmsI GCATC 4 cut(s) 144, 428, 523, 1154
BplI GAGNNNNNCTC 4 cut(s) 355, 387, 714, 746
Bpu1102I GCTNAGC 1 cut(s) 1010
Bsa29I ATCGAT 1 cut(s) 1037
BsaAI YACGTR 1 cut(s) 839
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 1215
BsaWI WCCGGW 1 cut(s) 685
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse1I ACTGG 1 cut(s) 513
BseCI ATCGAT 1 cut(s) 1037
BseDI CCNNGG 1 cut(s) 1215
BseGI GGATG 2 cut(s) 436, 943
BseLI CCNNNNNNNGG 1 cut(s) 508
BseMII CTCAG 2 cut(s) 115, 1001
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 1 cut(s) 1142
BseXI GCAGC 5 cut(s) 169, 400, 428, 437, 444
Bsh1236I CGCG 1 cut(s) 830
BshFI GGCC 1 cut(s) 238
BshVI ATCGAT 1 cut(s) 1037
BsiHKAI GWGCWC 1 cut(s) 1016
BsiHKCI CYCGRG 2 cut(s) 372, 958
BsiSI CCGG 2 cut(s) 239, 686
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 508
BsmAI GTCTC 6 cut(s) 104, 128, 164, 499, 961, 1132
BsmBI CGTCTC 1 cut(s) 1132
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 1 cut(s) 238
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 2 cut(s) 372, 958
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp143I GATC 8 cut(s) 44, 74, 104, 326, 521, 802, 1205, 1219
Bsp1720I GCTNAGC 1 cut(s) 1010
Bsp19I CCATGG 1 cut(s) 1215
BspACI CCGC 5 cut(s) 138, 368, 428, 473, 1046
BspANI GGCC 1 cut(s) 238
BspCNI CTCAG 2 cut(s) 114, 1002
BspDI ATCGAT 1 cut(s) 1037
BspFNI CGCG 1 cut(s) 830
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 1 cut(s) 1227
BspQI GCTCTTC 2 cut(s) 715, 1021
BspTNI GGTCTC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 368
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 1 cut(s) 1215
BssMI GATC 8 cut(s) 44, 74, 104, 326, 521, 802, 1205, 1219
BssT1I CCWWGG 1 cut(s) 1215
Bst4CI ACNGT 8 cut(s) 7, 56, 92, 256, 382, 710, 754, 947
Bst6I CTCTTC 5 cut(s) 354, 510, 534, 715, 1021
BstBAI YACGTR 1 cut(s) 839
BstDEI CTNAG 2 cut(s) 101, 1010
BstDSI CCRYGG 1 cut(s) 1215
BstF5I GGATG 2 cut(s) 436, 943
BstFNI CGCG 1 cut(s) 830
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 8 cut(s) 47, 77, 107, 329, 524, 805, 1208, 1222
BstMAI GTCTC 6 cut(s) 104, 128, 164, 499, 961, 1132
BstMBI GATC 8 cut(s) 44, 74, 104, 326, 521, 802, 1205, 1219
BstMWI GCNNNNNNNGC 5 cut(s) 21, 374, 422, 425, 434
BstNSI RCATGY 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 414
BstUI CGCG 1 cut(s) 830
BstV1I GCAGC 5 cut(s) 169, 400, 428, 437, 444
BstX2I RGATCY 5 cut(s) 104, 326, 802, 1205, 1219
BstYI RGATCY 5 cut(s) 104, 326, 802, 1205, 1219
Bsu15I ATCGAT 1 cut(s) 1037
BsuRI GGCC 1 cut(s) 238
BsuTUI ATCGAT 1 cut(s) 1037
BtgI CCRYGG 1 cut(s) 1215
BtgZI GCGATG 2 cut(s) 452, 458
BtsCI GGATG 2 cut(s) 436, 943
CaiI CAGNNNCTG 1 cut(s) 508
CfoI GCGC 1 cut(s) 413
ClaI ATCGAT 1 cut(s) 1037
Csp6I GTAC 1 cut(s) 832
CviAII CATG 3 cut(s) 16, 174, 1216
CviQI GTAC 1 cut(s) 832
DdeI CTNAG 2 cut(s) 101, 1010
DpnI GATC 8 cut(s) 46, 76, 106, 328, 523, 804, 1207, 1221
DpnII GATC 8 cut(s) 44, 74, 104, 326, 521, 802, 1205, 1219
EaeI YGGCCR 1 cut(s) 236
Eam1104I CTCTTC 5 cut(s) 354, 510, 534, 715, 1021
EarI CTCTTC 5 cut(s) 354, 510, 534, 715, 1021
Ecl136II GAGCTC 1 cut(s) 1014
Eco130I CCWWGG 1 cut(s) 1215
Eco24I GRGCYC 1 cut(s) 1016
Eco31I GGTCTC 1 cut(s) 128
Eco47III AGCGCT 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 1014
Eco57I CTGAAG 1 cut(s) 558
Eco72I CACGTG 1 cut(s) 839
Eco88I CYCGRG 2 cut(s) 372, 958
EcoICRI GAGCTC 1 cut(s) 1014
EcoT14I CCWWGG 1 cut(s) 1215
EcoT38I GRGCYC 1 cut(s) 1016
ErhI CCWWGG 1 cut(s) 1215
Esp3I CGTCTC 1 cut(s) 1132
FaeI CATG 3 cut(s) 19, 177, 1219
FaqI GGGAC 1 cut(s) 232
FatI CATG 3 cut(s) 15, 173, 1215
Fnu4HI GCNGC 7 cut(s) 158, 369, 414, 417, 426, 458, 474
FokI GGATG 2 cut(s) 443, 950
FriOI GRGCYC 1 cut(s) 1016
Fsp4HI GCNGC 7 cut(s) 158, 369, 414, 417, 426, 458, 474
FspBI CTAG 1 cut(s) 899
GlaI GCGC 1 cut(s) 412
GluI GCNGC 7 cut(s) 158, 369, 414, 417, 426, 458, 474
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 1 cut(s) 238
HapII CCGG 2 cut(s) 239, 686
HhaI GCGC 1 cut(s) 413
Hin1II CATG 3 cut(s) 19, 177, 1219
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HindIII AAGCTT 1 cut(s) 612
HinfI GANTC 4 cut(s) 818, 895, 905, 929
HpaII CCGG 2 cut(s) 239, 686
HphI GGTGA 1 cut(s) 871
Hpy166II GTNNAC 3 cut(s) 88, 277, 995
Hpy188I TCNGA 5 cut(s) 104, 121, 817, 823, 1186
Hpy188III TCNNGA 9 cut(s) 48, 502, 680, 713, 737, 758, 899, 1115, 1233
Hpy8I GTNNAC 3 cut(s) 88, 277, 995
HpyAV CCTTC 3 cut(s) 463, 773, 856
HpyCH4III ACNGT 8 cut(s) 7, 56, 92, 256, 382, 710, 754, 947
HpyCH4IV ACGT 3 cut(s) 28, 838, 1181
HpyCH4V TGCA 4 cut(s) 95, 157, 416, 460
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 374, 422, 425, 434
HpyF3I CTNAG 2 cut(s) 101, 1010
HpySE526I ACGT 3 cut(s) 28, 838, 1181
Hsp92II CATG 3 cut(s) 19, 177, 1219
HspAI GCGC 1 cut(s) 411
Kzo9I GATC 8 cut(s) 44, 74, 104, 326, 521, 802, 1205, 1219
LguI GCTCTTC 2 cut(s) 715, 1021
LmnI GCTCC 1 cut(s) 1129
LpnPI CCDG 9 cut(s) 63, 195, 252, 257, 494, 515, 693, 699, 722
Lsp1109I GCAGC 5 cut(s) 169, 400, 428, 437, 444
LweI GCATC 4 cut(s) 144, 428, 523, 1154
MaeI CTAG 1 cut(s) 899
MaeII ACGT 3 cut(s) 28, 838, 1181
MaeIII GTNAC 1 cut(s) 34
MalI GATC 8 cut(s) 46, 76, 106, 328, 523, 804, 1207, 1221
MbiI CCGCTC 1 cut(s) 368
MboI GATC 8 cut(s) 44, 74, 104, 326, 521, 802, 1205, 1219
MfeI CAATTG 1 cut(s) 477
MflI RGATCY 5 cut(s) 104, 326, 802, 1205, 1219
MhlI GDGCHC 1 cut(s) 1016
MluCI AATT 7 cut(s) 148, 242, 283, 347, 477, 644, 1085
MmeI TCCRAC 2 cut(s) 915, 1178
MnlI CCTC 9 cut(s) 208, 486, 634, 919, 990, 1053, 1111, 1120, 1164
MseI TTAA 6 cut(s) 60, 264, 659, 1064, 1091, 1103
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 2 cut(s) 239, 686
MunI CAATTG 1 cut(s) 477
MvnI CGCG 1 cut(s) 830
MwoI GCNNNNNNNGC 5 cut(s) 21, 374, 422, 425, 434
NcoI CCATGG 1 cut(s) 1215
NdeII GATC 8 cut(s) 44, 74, 104, 326, 521, 802, 1205, 1219
NlaIII CATG 3 cut(s) 19, 177, 1219
NspI RCATGY 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 372
PciI ACATGT 1 cut(s) 173
PciSI GCTCTTC 2 cut(s) 715, 1021
PfeI GAWTC 4 cut(s) 818, 895, 905, 929
PflMI CCANNNNNTGG 1 cut(s) 508
PkrI GCNGC 7 cut(s) 159, 370, 415, 418, 427, 459, 475
PmaCI CACGTG 1 cut(s) 839
PmlI CACGTG 1 cut(s) 839
Ppu21I YACGTR 1 cut(s) 839
PscI ACATGT 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 1016
Psp1406I AACGTT 1 cut(s) 28
PspCI CACGTG 1 cut(s) 839
PspXI VCTCGAGB 1 cut(s) 372
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 508
PsuI RGATCY 5 cut(s) 104, 326, 802, 1205, 1219
RsaI GTAC 1 cut(s) 833
RsaNI GTAC 1 cut(s) 832
SacI GAGCTC 1 cut(s) 1016
SapI GCTCTTC 2 cut(s) 715, 1021
SaqAI TTAA 6 cut(s) 60, 264, 659, 1064, 1091, 1103
SatI GCNGC 7 cut(s) 158, 369, 414, 417, 426, 458, 474
Sau3AI GATC 8 cut(s) 44, 74, 104, 326, 521, 802, 1205, 1219
SduI GDGCHC 1 cut(s) 1016
SfaNI GCATC 4 cut(s) 144, 428, 523, 1154
SfcI CTRYAG 1 cut(s) 414
Sfr274I CTCGAG 1 cut(s) 372
SlaI CTCGAG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 372
SmoI CTYRAG 1 cut(s) 372
Sse9I AATT 7 cut(s) 148, 242, 283, 347, 477, 644, 1085
SsiI CCGC 5 cut(s) 138, 368, 428, 473, 1046
SspMI CTAG 1 cut(s) 899
SstI GAGCTC 1 cut(s) 1016
StyI CCWWGG 1 cut(s) 1215
TaaI ACNGT 8 cut(s) 7, 56, 92, 256, 382, 710, 754, 947
TaiI ACGT 3 cut(s) 31, 841, 1184
TaqI TCGA 7 cut(s) 373, 390, 524, 630, 759, 970, 1037
TasI AATT 7 cut(s) 148, 242, 283, 347, 477, 644, 1085
TauI GCSGC 2 cut(s) 371, 476
TfiI GAWTC 4 cut(s) 818, 895, 905, 929
Tru1I TTAA 6 cut(s) 60, 264, 659, 1064, 1091, 1103
Tru9I TTAA 6 cut(s) 60, 264, 659, 1064, 1091, 1103
TseI GCWGC 5 cut(s) 157, 413, 416, 425, 457
TspDTI ATGAA 6 cut(s) 32, 105, 276, 447, 954, 1092
Van91I CCANNNNNTGG 1 cut(s) 508
XapI RAATTY 1 cut(s) 242
XbaI TCTAGA 1 cut(s) 898
XceI RCATGY 1 cut(s) 177
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 372
XspI CTAG 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.