Rw4G016510

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005611
Physical Location & Seq
Reverse (-)
37653771 .. 37657646
3876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016510.1

Sequence Viewer

Length: 2421 bp
ATGGCATGCCAAATGATACATTCATGGACATTCAGTGGGCTTGTGGGTGCATTTCTTGATCTTGCAATAGCCTATCTCTTACTCTGTGCCTCAGCTCTTGCGTTTTTTACCTCGAAGTTTTTGGATGTCTTTGGATTAAGTCTTCCATGCCCTTGTGATGGGCTGTTTGGGAACCCAAAGAATAATTACTGCTTCCAGAAGCAGTTAGTGGATGGCCCATCTGAGAAAATCGGTGGAGTTCAGTTGCAGCTGAAGAGCAAGTTCCCTTTCGATGTAATGTGGAGTGAAGACCCACATTTTCATGCAAAGTCGAAGTCGGATCATGAGAATGGGCACTTTGTGTTTGAAGGTGAAGCATCGTGTAGCTCTTATTCGGATGGGAAGTTGCTAGATGTGGCTGGGAGGGCTTGCTCTGTTTCGGGGAATGAAATGAGTTTTGAGTCTGGTACGGTGAATTTGGAAAAATGCTTTGATCATAAGGGAAAGAAGGTTGGTGGTCGAAGGCCAAGTCATAGTCTGCGCCGCCGGCGCAAGGGGGCCTCTTTGGATTATGGAAAGCTGTTTTCAGTTTCCTCAAGTGATACGGTACAGTCAGATTCCCAAGACATGGCTGGACCTCCTTACAGAAATAGTAAAATGGGGGACGAGGTTACTGAAGTTCCCGTCAATTCAGGAAGGCCAAATGGCATGGAAGCTCCAACAGACACTGGTGGGCCAGAAAGAGTTTCACATCTTGAGTTGAATGAACATGTTGGTAAAACTAAATCAATTCAGAATGATGCTTCTTCTGTTGAAAATTTAGGCTGCAATGAGCAAGAGAAGCCAGTTTATGATGGTAATGATAAAACTATGGTTCGGGTATTGGAACAAGCACTAGACGAAGAGCATACTGCTCGTACTGCTCTTTATTATGAATTGGAGAAGGAGAGAAGTGCTGCTGCTACTGCGGCAGATGAGGCCATGGCAATGATATTGCGTCTGCAAGAAGAGAAGGCATCAATTGAAATGGAGGCAAGGCAATACCAGAGGATGATACAGGAAAAATCAGCTTATGATGCTGAGGAAATGAACATTCTCAAAGAGATCTTACTTAGGAGAGAAAGGGAAAAGCATTTCTTGGAGAAGGAAGTTGAATACTACAGGCAAATACTTTTTGGAACGATCAATATTTCTCTGCATTCAAGTGAAGAGCTGGTGTCGATGCAGGAGCGGACAAACTATTCAATTAGTGAGAAGTCAAAGTTGAAAATGGCAAATAGTTCTCCAGATTACGGGGCCCTATCAAGTGACTCACAAAATCGCACTCTGGCCTTGGGGAAAGAACTACCAGTTCTGGATTTGGAAGAAGATGACACTTCGAAGCATCCATTCACATACTTAAATAGTAGGTATGAGATCAGTCACGAGTTTCAAGAGAAGGGAATGGTGTCTGTGGATGAGAGACCAGTTTCTCGGGGAAAAGAGGTCCAGGTAGTGAATGCTGAGGGACTTGAATTGGTTGAAAAAACTATTCCTCCGAACGAACAGGAACTTGCTTTAGCTGGTAGCAATGTGGATCATGGAAGCAAAGATCCACCTAGCTTAACGTCTAATACAGAGCCTCGTGTTCATGATATTCATGTTATTGACGATAAGTCTGATTTGTATAATGAAAAGAGTGCAGAGAAAAGTGAACAGCTAGTAGCAAATGCTACCTTAGATATTTCTGAAAAACACAAGAGTATGACAGGGTTGGGAACCGAGCTTAAAGTGCACAAAGTTGGTTCAGACAGTGGATTGCCAATAGTGGATGGCTCTCAAGGCAAAGCTTTACCTTACAATATGCGGAGGAATTCCATGTCTGAGGTTGATTATGAAAGATCGAAAATAGACAGTAAACTGCACCGAGGTGGTCCAGACATGGCACCTAATTGCATGCGGAGGAATTCCATGTCTGCAATTGATTATGACAGATGGAAAATTGACAACAAAGTGGACAGAGGTAGCTCAGACACGGAACCTAAAGATGTGCGGAGGAATTCCATGACTGCAGTTGATTATGAAAGATGGAAAATGGCCAATAAATCATACAGAGGTAGTTCAGACACGTCGCCATCACCTTCTGTCATGCGAAGAAATTCCATGTCTGCAGTTGATTATGAAAGATGCAAACTTGACAATGAAGTTGAGTGGCTTAGAGAAAGGTTAAGGATTGTGCAAGAGGGAAGAGAGAAGCTCAACCTCTCAGTGGGCCACAGAGAAAGGGAAAAAGTTCAGCTGAAACTTTTGGAAGATATAGCAGGCCAACTCCAAGAGATTCGGCAATTGACGGAACCTGGAAAGGTCAAGTGCCAGGCTGCATTGCCGCCTCCATCATCTAAGGTTATGTCAAAGAAAAGACGATGGCGAACTTTGTCTCTAGGAGTGCATAGAAGTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

806

Amino Acids

90.51

Weight (kDa)

5.91

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 288 - 377 9.9e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1903
AccB7I CCANNNNNTGG 1 cut(s) 607
AccBSI CCGCTC 1 cut(s) 1210
AciI CCGC 7 cut(s) 523, 947, 1210, 1825, 1918, 2011, 2345
AclWI GGATC 3 cut(s) 327, 1563, 1565
AcoI YGGCCR 1 cut(s) 2055
AcsI RAATTY 6 cut(s) 454, 796, 1831, 1924, 2017, 2116
AcuI CTGAAG 2 cut(s) 272, 675
AdeI CACNNNGTG 1 cut(s) 340
AfaI GTAC 4 cut(s) 448, 588, 898, 2416
AfiI CCNNNNNNNGG 5 cut(s) 158, 532, 607, 1271, 2227
AflIII ACRYGT 2 cut(s) 748, 2085
AhdI GACNNNNNGTC 1 cut(s) 1633
AjiI CACGTC 1 cut(s) 2088
AjnI CCWGG 3 cut(s) 1467, 2314, 2331
AleI CACNNNNGTG 1 cut(s) 1887
Alw21I GWGCWC 1 cut(s) 1755
Alw26I GTCTC 2 cut(s) 1435, 2400
Alw44I GTGCAC 1 cut(s) 1751
AlwI GGATC 3 cut(s) 327, 1563, 1565
AlwNI CAGNNNCTG 1 cut(s) 707
Ama87I CYCGRG 1 cut(s) 1452
ApaI GGGCCC 1 cut(s) 1279
ApaLI GTGCAC 1 cut(s) 1751
ApeKI GCWGC 5 cut(s) 247, 804, 935, 938, 2336
ApoI RAATTY 6 cut(s) 454, 796, 1831, 1924, 2017, 2116
ArsI GACNNNNNNTTYG 4 cut(s) 299, 331, 493, 525
Asp700I GAANNNNTTC 2 cut(s) 2116, 2250
AspLEI GCGC 2 cut(s) 522, 531
AspS9I GGNCC 9 cut(s) 215, 537, 614, 713, 1275, 1276, 1465, 1892, 2230
AsuHPI GGTGA 3 cut(s) 362, 463, 2088
AsuII TTCGAA 1 cut(s) 1358
AvaI CYCGRG 1 cut(s) 1452
AvaII GGWCC 3 cut(s) 614, 1465, 1892
BaeGI GKGCMC 3 cut(s) 336, 1279, 1755
BalI TGGCCA 1 cut(s) 2057
BanI GGYRCC 1 cut(s) 1903
BanII GRGCYC 1 cut(s) 1279
BauI CACGAG 2 cut(s) 1403, 1602
BbsI GAAGAC 2 cut(s) 134, 294
Bbv12I GWGCWC 1 cut(s) 1755
BbvCI CCTCAGC 3 cut(s) 91, 1059, 1482
BbvI GCAGC 5 cut(s) 259, 791, 922, 925, 2323
BcgI CGANNNNNNTGC 2 cut(s) 875, 909
BciT130I CCWGG 3 cut(s) 1469, 2316, 2333
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 2 cut(s) 1435, 2400
BfaI CTAG 5 cut(s) 389, 875, 1578, 1679, 2399
BfmI CTRYAG 3 cut(s) 1138, 2028, 2127
BglII AGATCT 1 cut(s) 1083
BisI GCNGC 8 cut(s) 248, 523, 805, 936, 939, 948, 2337, 2345
BlsI GCNGC 8 cut(s) 249, 524, 806, 937, 940, 949, 2338, 2346
Bme1390I CCNGG 3 cut(s) 1469, 2316, 2333
Bme18I GGWCC 3 cut(s) 614, 1465, 1892
BmeRI GACNNNNNGTC 1 cut(s) 1633
BmeT110I CYCGRG 1 cut(s) 1452
BmgBI CACGTC 1 cut(s) 2088
BmgT120I GGNCC 9 cut(s) 215, 537, 614, 713, 1275, 1276, 1465, 1892, 2230
BmiI GGNNCC 8 cut(s) 173, 538, 1276, 1277, 1738, 1905, 1998, 2313
BmrFI CCNGG 3 cut(s) 1469, 2316, 2333
BmsI GCATC 7 cut(s) 365, 769, 1004, 1045, 1191, 1372, 2135
BpiI GAAGAC 2 cut(s) 134, 294
BplI GAGNNNNNCTC 2 cut(s) 2199, 2231
BpmI CTGGAG 1 cut(s) 1248
Bpu10I CCTNAGC 3 cut(s) 91, 1059, 1482
Bpu14I TTCGAA 1 cut(s) 1358
BpuEI CTTGAG 3 cut(s) 559, 755, 1782
BsaI GGTCTC 1 cut(s) 1435
BsaJI CCNNGG 3 cut(s) 960, 1311, 1885
BsaXI ACNNNNNCTCC 2 cut(s) 1498, 1528
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 532, 607, 1271, 2227
Bse118I RCCGGY 1 cut(s) 525
Bse1I ACTGG 4 cut(s) 712, 824, 1328, 1445
Bse3DI GCAATG 4 cut(s) 814, 972, 1555, 2339
BseBI CCWGG 3 cut(s) 1469, 2316, 2333
BseDI CCNNGG 3 cut(s) 960, 1311, 1885
BseGI GGATG 7 cut(s) 130, 217, 382, 1035, 1363, 1441, 1795
BseLI CCNNNNNNNGG 5 cut(s) 158, 532, 607, 1271, 2227
BseMI GCAATG 4 cut(s) 814, 972, 1555, 2339
BseMII CTCAG 7 cut(s) 105, 213, 1050, 1473, 1833, 2001, 2238
BseNI ACTGG 4 cut(s) 712, 824, 1328, 1445
BseSI GKGCMC 3 cut(s) 336, 1279, 1755
BseXI GCAGC 5 cut(s) 259, 791, 922, 925, 2323
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 2 cut(s) 1680, 1865
BshNI GGYRCC 1 cut(s) 1903
BsiHKAI GWGCWC 1 cut(s) 1755
BsiHKCI CYCGRG 1 cut(s) 1452
BsiSI CCGG 1 cut(s) 526
BslFI GGGAC 2 cut(s) 656, 1500
BslI CCNNNNNNNGG 5 cut(s) 158, 532, 607, 1271, 2227
BsmAI GTCTC 2 cut(s) 1435, 2400
BsmFI GGGAC 2 cut(s) 656, 1500
BsmI GAATGC 2 cut(s) 1177, 1483
Bso31I GGTCTC 1 cut(s) 1435
BsoBI CYCGRG 1 cut(s) 1452
Bsp119I TTCGAA 1 cut(s) 1358
Bsp120I GGGCCC 1 cut(s) 1275
Bsp1286I GDGCHC 3 cut(s) 336, 1279, 1755
Bsp143I GATC 9 cut(s) 58, 319, 472, 1083, 1161, 1395, 1555, 1570, 1859
Bsp19I CCATGG 1 cut(s) 960
BspACI CCGC 7 cut(s) 523, 947, 1210, 1825, 1918, 2011, 2345
BspCNI CTCAG 7 cut(s) 104, 214, 1051, 1474, 1834, 2000, 2237
BspHI TCATGA 2 cut(s) 322, 1609
BspLI GGNNCC 8 cut(s) 173, 538, 1276, 1277, 1738, 1905, 1998, 2313
BspMAI CTGCAG 2 cut(s) 2032, 2131
BspPI GGATC 3 cut(s) 327, 1563, 1565
BspQI GCTCTTC 3 cut(s) 248, 876, 1182
BspT104I TTCGAA 1 cut(s) 1358
BspT107I GGYRCC 1 cut(s) 1903
BspTNI GGTCTC 1 cut(s) 1435
BsrBI CCGCTC 1 cut(s) 1210
BsrDI GCAATG 4 cut(s) 814, 972, 1555, 2339
BsrFI RCCGGY 1 cut(s) 525
BsrI ACTGG 4 cut(s) 712, 824, 1328, 1445
BssAI RCCGGY 1 cut(s) 525
BssECI CCNNGG 3 cut(s) 960, 1311, 1885
BssMI GATC 9 cut(s) 58, 319, 472, 1083, 1161, 1395, 1555, 1570, 1859
BssSI CACGAG 2 cut(s) 1403, 1602
BssT1I CCWWGG 2 cut(s) 960, 1311
Bst2BI CACGAG 2 cut(s) 1403, 1602
Bst2UI CCWGG 3 cut(s) 1469, 2316, 2333
Bst4CI ACNGT 5 cut(s) 451, 586, 591, 1772, 1874
Bst6I CTCTTC 5 cut(s) 248, 876, 981, 1182, 2200
BstBI TTCGAA 1 cut(s) 1358
BstC8I GCNNGC 5 cut(s) 7, 409, 527, 1916, 2281
BstDSI CCRYGG 1 cut(s) 960
BstF5I GGATG 7 cut(s) 130, 217, 382, 1035, 1363, 1441, 1795
BstHHI GCGC 2 cut(s) 522, 531
BstKTI GATC 9 cut(s) 61, 322, 475, 1086, 1164, 1398, 1558, 1573, 1862
BstMAI GTCTC 2 cut(s) 1435, 2400
BstMBI GATC 9 cut(s) 58, 319, 472, 1083, 1161, 1395, 1555, 1570, 1859
BstNI CCWGG 3 cut(s) 1469, 2316, 2333
BstNSI RCATGY 3 cut(s) 9, 752, 1918
BstSCI CCNGG 3 cut(s) 1467, 2314, 2331
BstSFI CTRYAG 3 cut(s) 1138, 2028, 2127
BstSLI GKGCMC 3 cut(s) 336, 1279, 1755
BstV1I GCAGC 5 cut(s) 259, 791, 922, 925, 2323
BstV2I GAAGAC 2 cut(s) 134, 294
BstX2I RGATCY 2 cut(s) 1083, 1570
BstYI RGATCY 2 cut(s) 1083, 1570
BtgI CCRYGG 1 cut(s) 960
BtrI CACGTC 1 cut(s) 2088
BtsCI GGATG 7 cut(s) 130, 217, 382, 1035, 1363, 1441, 1795
BtsIMutI CAGTG 4 cut(s) 40, 705, 1777, 2232
Cac8I GCNNGC 5 cut(s) 7, 409, 527, 1916, 2281
CaiI CAGNNNCTG 1 cut(s) 707
CciI TCATGA 2 cut(s) 322, 1609
CfoI GCGC 2 cut(s) 522, 531
Cfr10I RCCGGY 1 cut(s) 525
Cfr13I GGNCC 9 cut(s) 215, 537, 614, 713, 1275, 1276, 1465, 1892, 2230
CseI GACGC 1 cut(s) 965
Csp6I GTAC 4 cut(s) 447, 587, 897, 2415
CviQI GTAC 4 cut(s) 447, 587, 897, 2415
DpnI GATC 9 cut(s) 60, 321, 474, 1085, 1163, 1397, 1557, 1572, 1861
DpnII GATC 9 cut(s) 58, 319, 472, 1083, 1161, 1395, 1555, 1570, 1859
DraIII CACNNNGTG 1 cut(s) 340
DriI GACNNNNNGTC 1 cut(s) 1633
EaeI YGGCCR 1 cut(s) 2055
Eam1104I CTCTTC 5 cut(s) 248, 876, 981, 1182, 2200
Eam1105I GACNNNNNGTC 1 cut(s) 1633
EarI CTCTTC 5 cut(s) 248, 876, 981, 1182, 2200
Eco130I CCWWGG 2 cut(s) 960, 1311
Eco24I GRGCYC 1 cut(s) 1279
Eco31I GGTCTC 1 cut(s) 1435
Eco47I GGWCC 3 cut(s) 614, 1465, 1892
Eco57I CTGAAG 2 cut(s) 272, 675
Eco88I CYCGRG 1 cut(s) 1452
EcoO109I RGGNCCY 3 cut(s) 537, 1275, 1276
EcoRI GAATTC 3 cut(s) 1831, 1924, 2017
EcoRII CCWGG 3 cut(s) 1467, 2314, 2331
EcoT14I CCWWGG 2 cut(s) 960, 1311
EcoT38I GRGCYC 1 cut(s) 1279
ErhI CCWWGG 2 cut(s) 960, 1311
FalI AAGNNNNNCTT 2 cut(s) 1100, 1132
FaqI GGGAC 2 cut(s) 656, 1500
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 8 cut(s) 248, 523, 805, 936, 939, 948, 2337, 2345
FokI GGATG 7 cut(s) 137, 224, 389, 1042, 1350, 1448, 1802
FriOI GRGCYC 1 cut(s) 1279
Fsp4HI GCNGC 8 cut(s) 248, 523, 805, 936, 939, 948, 2337, 2345
FspBI CTAG 5 cut(s) 389, 875, 1578, 1679, 2399
GlaI GCGC 2 cut(s) 521, 530
GluI GCNGC 8 cut(s) 248, 523, 805, 936, 939, 948, 2337, 2345
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 1248
HapII CCGG 1 cut(s) 526
HgaI GACGC 1 cut(s) 965
HhaI GCGC 2 cut(s) 522, 531
Hin6I GCGC 2 cut(s) 520, 529
HinP1I GCGC 2 cut(s) 520, 529
HindIII AAGCTT 1 cut(s) 1806
HinfI GANTC 4 cut(s) 440, 596, 1289, 2296
HpaII CCGG 1 cut(s) 526
HphI GGTGA 3 cut(s) 362, 463, 2088
Hpy166II GTNNAC 4 cut(s) 1673, 1753, 1877, 1975
Hpy8I GTNNAC 4 cut(s) 1673, 1753, 1877, 1975
Hpy99I CGWCG 1 cut(s) 2092
HpyAV CCTTC 9 cut(s) 341, 481, 495, 669, 916, 985, 1117, 1411, 2109
HpyCH4III ACNGT 5 cut(s) 451, 586, 591, 1772, 1874
HpyCH4IV ACGT 2 cut(s) 1586, 2087
HpySE526I ACGT 2 cut(s) 1586, 2087
HspAI GCGC 2 cut(s) 520, 529
KroI GCCGGC 1 cut(s) 525
KroNI GCCGGC 1 cut(s) 527
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 9 cut(s) 58, 319, 472, 1083, 1161, 1395, 1555, 1570, 1859
LguI GCTCTTC 3 cut(s) 248, 876, 1182
LmnI GCTCC 2 cut(s) 700, 1207
Lsp1109I GCAGC 5 cut(s) 259, 791, 922, 925, 2323
LweI GCATC 7 cut(s) 365, 769, 1004, 1045, 1191, 1372, 2135
MaeI CTAG 5 cut(s) 389, 875, 1578, 1679, 2399
MaeII ACGT 2 cut(s) 1586, 2087
MaeIII GTNAC 3 cut(s) 649, 1286, 1400
MalI GATC 9 cut(s) 60, 321, 474, 1085, 1163, 1397, 1557, 1572, 1861
MbiI CCGCTC 1 cut(s) 1210
MboI GATC 9 cut(s) 58, 319, 472, 1083, 1161, 1395, 1555, 1570, 1859
MfeI CAATTG 3 cut(s) 999, 1938, 2303
MflI RGATCY 2 cut(s) 1083, 1570
MhlI GDGCHC 3 cut(s) 336, 1279, 1755
MlsI TGGCCA 1 cut(s) 2057
MluNI TGGCCA 1 cut(s) 2057
MlyI GAGTC 2 cut(s) 449, 1283
MmeI TCCRAC 2 cut(s) 297, 722
Mox20I TGGCCA 1 cut(s) 2057
MreI CGCCGGCG 1 cut(s) 525
MroNI GCCGGC 1 cut(s) 525
MroXI GAANNNNTTC 2 cut(s) 2116, 2250
MscI TGGCCA 1 cut(s) 2057
MseI TTAA 5 cut(s) 137, 1379, 1583, 1746, 2186
MslI CAYNNNNRTG 5 cut(s) 300, 327, 965, 1182, 1887
Msp20I TGGCCA 1 cut(s) 2057
MspA1I CMGCKG 2 cut(s) 250, 2257
MspI CCGG 1 cut(s) 526
MspR9I CCNGG 3 cut(s) 1469, 2316, 2333
MunI CAATTG 3 cut(s) 999, 1938, 2303
Mva1269I GAATGC 2 cut(s) 1177, 1483
MvaI CCWGG 3 cut(s) 1469, 2316, 2333
NaeI GCCGGC 1 cut(s) 527
NcoI CCATGG 1 cut(s) 960
NdeII GATC 9 cut(s) 58, 319, 472, 1083, 1161, 1395, 1555, 1570, 1859
NgoMIV GCCGGC 1 cut(s) 525
NlaIV GGNNCC 8 cut(s) 173, 538, 1276, 1277, 1738, 1905, 1998, 2313
NmuCI GTSAC 2 cut(s) 1286, 1400
NspI RCATGY 3 cut(s) 9, 752, 1918
NspV TTCGAA 1 cut(s) 1358
OliI CACNNNNGTG 1 cut(s) 1887
PaeI GCATGC 2 cut(s) 9, 1918
PagI TCATGA 2 cut(s) 322, 1609
PciI ACATGT 1 cut(s) 748
PciSI GCTCTTC 3 cut(s) 248, 876, 1182
PctI GAATGC 2 cut(s) 1177, 1483
PdiI GCCGGC 1 cut(s) 527
PdmI GAANNNNTTC 2 cut(s) 2116, 2250
PfeI GAWTC 2 cut(s) 596, 2296
PflMI CCANNNNNTGG 1 cut(s) 607
PkrI GCNGC 8 cut(s) 249, 524, 806, 937, 940, 949, 2338, 2346
PleI GAGTC 2 cut(s) 448, 1283
PpsI GAGTC 2 cut(s) 448, 1283
PscI ACATGT 1 cut(s) 748
Psp6I CCWGG 3 cut(s) 1467, 2314, 2331
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 3 cut(s) 1467, 2314, 2331
PspN4I GGNNCC 8 cut(s) 173, 538, 1276, 1277, 1738, 1905, 1998, 2313
PspOMI GGGCCC 1 cut(s) 1275
PspPI GGNCC 9 cut(s) 215, 537, 614, 713, 1275, 1276, 1465, 1892, 2230
PstI CTGCAG 2 cut(s) 2032, 2131
PstNI CAGNNNCTG 1 cut(s) 707
PsuI RGATCY 2 cut(s) 1083, 1570
PvuII CAGCTG 2 cut(s) 250, 2257
RsaI GTAC 4 cut(s) 448, 588, 898, 2416
RsaNI GTAC 4 cut(s) 447, 587, 897, 2415
RseI CAYNNNNRTG 5 cut(s) 300, 327, 965, 1182, 1887
SapI GCTCTTC 3 cut(s) 248, 876, 1182
SaqAI TTAA 5 cut(s) 137, 1379, 1583, 1746, 2186
SatI GCNGC 8 cut(s) 248, 523, 805, 936, 939, 948, 2337, 2345
Sau3AI GATC 9 cut(s) 58, 319, 472, 1083, 1161, 1395, 1555, 1570, 1859
Sau96I GGNCC 9 cut(s) 215, 537, 614, 713, 1275, 1276, 1465, 1892, 2230
SchI GAGTC 2 cut(s) 449, 1283
ScrFI CCNGG 3 cut(s) 1469, 2316, 2333
SduI GDGCHC 3 cut(s) 336, 1279, 1755
SfaNI GCATC 7 cut(s) 365, 769, 1004, 1045, 1191, 1372, 2135
SfcI CTRYAG 3 cut(s) 1138, 2028, 2127
SfuI TTCGAA 1 cut(s) 1358
SgrAI CRCCGGYG 1 cut(s) 525
SinI GGWCC 3 cut(s) 614, 1465, 1892
SmiMI CAYNNNNRTG 5 cut(s) 300, 327, 965, 1182, 1887
SmlI CTYRAG 3 cut(s) 574, 734, 1797
SmoI CTYRAG 3 cut(s) 574, 734, 1797
SphI GCATGC 2 cut(s) 9, 1918
SsiI CCGC 7 cut(s) 523, 947, 1210, 1825, 1918, 2011, 2345
SspI AATATT 1 cut(s) 1168
SspMI CTAG 5 cut(s) 389, 875, 1578, 1679, 2399
StyD4I CCNGG 3 cut(s) 1467, 2314, 2331
StyI CCWWGG 2 cut(s) 960, 1311
TaaI ACNGT 5 cut(s) 451, 586, 591, 1772, 1874
TaiI ACGT 2 cut(s) 1589, 2090
TaqI TCGA 7 cut(s) 113, 270, 311, 499, 1199, 1358, 1862
TauI GCSGC 3 cut(s) 525, 950, 2347
TfiI GAWTC 2 cut(s) 596, 2296
Tru1I TTAA 5 cut(s) 137, 1379, 1583, 1746, 2186
Tru9I TTAA 5 cut(s) 137, 1379, 1583, 1746, 2186
TscAI CASTG 4 cut(s) 40, 712, 1777, 2232
TseFI GTSAC 2 cut(s) 1286, 1400
TseI GCWGC 5 cut(s) 247, 804, 935, 938, 2336
Tsp45I GTSAC 2 cut(s) 1286, 1400
TspGWI ACGGA 2 cut(s) 2009, 2324
TspRI CASTG 4 cut(s) 40, 712, 1777, 2232
Van91I CCANNNNNTGG 1 cut(s) 607
VneI GTGCAC 1 cut(s) 1751
VpaK11BI GGWCC 3 cut(s) 614, 1465, 1892
XapI RAATTY 6 cut(s) 454, 796, 1831, 1924, 2017, 2116
XceI RCATGY 3 cut(s) 9, 752, 1918
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmnI GAANNNNTTC 2 cut(s) 2116, 2250
XspI CTAG 5 cut(s) 389, 875, 1578, 1679, 2399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.