AT4G13630

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005611
Physical Location & Seq
Reverse (-)
7933684 .. 7936565
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13630.1

Sequence Viewer

Length: 1710 bp
ATGCGTTGCCAGGAGGTAAAATCATGGACATTTAAAGGTTTAGTGGCTGCGTTTATCGATCTTTCCGTTGCGTTTTCGCTACTTTGCGCATCGTTTATCGTTTATGTAACATCGAAGTTCCTCGGTTTATTTGGTTTAGACCTACCATGTCCTTGTGATGGTCTATACAGTGAGTGTTTTCAAGAGAGTTTAAGGAATCTTCCGGTTAAAAAGATCTCTTCTGTTCAAAGATCTGTTAAAAATAGAACACCTTTTGATTCAATTCTGTATAATGGTGGAAAGAAGAGAAAATGTGAGCGTAGACGTGTTCAGTTGGAAGATGAGGTATCTAGTACTACTCCTTCTGTTGGAAAGTTTGAGAATAAGGCTTCAGGGTTCGATCTTTTAACTGCGCAAAGTTTGAAAAAAGGAAGCTTTAAAGTTAAGAGTAAGCGGCTTAGCTTCCATAGGTCGCCGTATGGTTTCAAGAACCATTACCAAAGTTGTTTAGGCCTTAAGAGCTTTGAGGGATCATATGATGAAAATGATCCTCTTCTTGTGAACTCCAATGATAGTGGAAAAGCATTGGAGGATGTGTCGTTGCGGAAAAGTGTTTCACTAAGTTCTGTTGGGTGTGGAGTGGGAGAGGGTGCATGCAGTTCCCCTGGTATGGTACAGAGGACGGTTGAGATGTCAGAACAAGTGCTTGGGGAAGAACGAGCTGCACGTGCTTCGTTAGCTCTTGAACTTGAGAAAGAGAGAAATGCTGCTGCATCTGCTGCAGATGAGGCTTTAGGTATGATATTACGGTTGCAAGAAGAAAAGGCGTCGATAGAGATGGAAGCTAGGCAATATCAGCGGATGATAGAAGAAAAGTCTGCTTTTGATGCGGAAGAGATGAGTATTCTTAAAGAAATTTTGCTTAGGAGGGAGAGGGAGAAACATTTTCTGGAGAAGGAAGTTGATACCTACAGGCAAATGTTTCTTGAGACTGAGCAACCTCACAATACTCCAGATTCTAAACCAGCTCAAATCGAAAGATTGCAGACGCCGCAACAGATTACTGAACCATGGGATGACATGGAGACTGTGAATGTCTCTTCTGGATTTGAGATCTTCACTAATCAGATGGACAATACTGAGTGTGATCAGTCAAGAAGTGAGCCATTTGATGCCCTGAGTGATATAGAACTGAAAGAAAGAGAGGAAGGTGAAACTTTATATGCAGAACTAGTATCAAGAACTAGCGACATTGCTGTTTCTAAGAAGCTTTGTGAGGACCCTCACGACATTGATTGTCATGTACATGACATCCATGTAGTGACTGATGAAGATAACAAGGGGCAACTGAATGTTCCTTCTGACCATGCCACTCAGGATCTGAAACTGGACAGATCACAAAGTGTTTCAGATACAAGTTATGCATTTCCACAAGGGAAAAGTAACATGTCTACCAATATGCGAAGAAATTCAATGTCAGCTATTGACTATGAAAGACTGAAAATAGAGAGTGAAGTTGGGTTGCTGAGAGGTCGGTTAAGAGCTGTACAAAAGGGAAGAGAGAAGATCAGTTTTTCTTCCAAAGATCAGAGCAAATCGCAGGTACAGCGTTGTGGAGATAAAACAAGCCGGTTTTGGGAGGCTAGAAGATCTGGACATCTTGACTCTTCTAGCCCTTCATCCACAATGGTAAAGGCTATGTCTATGCCACTGGACCTGCATAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

569

Amino Acids

64.02

Weight (kDa)

5.68

Isoelectric Point (pI)

61.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 227 - 314 4.3e-30 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 88, 393
Acc36I ACCTGC 2 cut(s) 1570, 1704
AccI GTMKAC 2 cut(s) 301, 1430
AciI CCGC 5 cut(s) 433, 583, 838, 869, 1031
AclWI GGATC 3 cut(s) 517, 521, 1365
AcsI RAATTY 2 cut(s) 894, 1447
AcuI CTGAAG 1 cut(s) 354
AcvI CACGTG 1 cut(s) 707
AcyI GRCGYC 2 cut(s) 806, 1028
AdeI CACNNNGTG 1 cut(s) 1382
AfaI GTAC 5 cut(s) 334, 654, 1284, 1527, 1584
AfiI CCNNNNNNNGG 4 cut(s) 158, 347, 649, 1615
AflII CTTAAG 1 cut(s) 494
AflIII ACRYGT 2 cut(s) 304, 1425
AgsI TTSAA 7 cut(s) 182, 227, 261, 403, 466, 725, 1452
AhlI ACTAGT 1 cut(s) 1210
AjiI CACGTC 1 cut(s) 305
AjnI CCWGG 2 cut(s) 9, 643
AjuI GAANNNNNNNTTGG 2 cut(s) 669, 701
Alw26I GTCTC 3 cut(s) 962, 1058, 1081
AlwI GGATC 3 cut(s) 517, 521, 1365
AlwNI CAGNNNCTG 1 cut(s) 1360
AoxI GGCC 1 cut(s) 490
ApeKI GCWGC 5 cut(s) 47, 701, 746, 749, 758
ApoI RAATTY 2 cut(s) 894, 1447
Asp700I GAANNNNTTC 1 cut(s) 1447
AspLEI GCGC 2 cut(s) 89, 394
AspS9I GGNCC 2 cut(s) 1258, 1693
AsuHPI GGTGA 1 cut(s) 1202
AvaII GGWCC 2 cut(s) 1258, 1693
BarI GAAGNNNNNNTAC 2 cut(s) 325, 357
BbrPI CACGTG 1 cut(s) 707
BbvI GCAGC 5 cut(s) 34, 688, 733, 736, 745
BccI CCATC 3 cut(s) 152, 811, 1102
BceAI ACGGC 1 cut(s) 439
BcgI CGANNNNNNTGC 2 cut(s) 693, 727
BciT130I CCWGG 2 cut(s) 11, 645
BclI TGATCA 1 cut(s) 1126
BcoDI GTCTC 3 cut(s) 962, 1058, 1081
BcuI ACTAGT 1 cut(s) 1210
BfaI CTAG 6 cut(s) 330, 825, 1211, 1224, 1623, 1650
BfmI CTRYAG 2 cut(s) 759, 949
BfrI CTTAAG 1 cut(s) 494
BfuAI ACCTGC 2 cut(s) 1570, 1704
BglII AGATCT 4 cut(s) 213, 230, 1092, 1628
BisI GCNGC 7 cut(s) 48, 434, 702, 747, 750, 759, 1031
BlpI GCTNAGC 1 cut(s) 437
BlsI GCNGC 7 cut(s) 49, 435, 703, 748, 751, 760, 1032
BmcAI AGTACT 1 cut(s) 334
Bme1390I CCNGG 2 cut(s) 11, 645
Bme18I GGWCC 2 cut(s) 1258, 1693
BmgBI CACGTC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 1258, 1693
BmiI GGNNCC 1 cut(s) 1260
BmrFI CCNGG 2 cut(s) 11, 645
BmsI GCATC 4 cut(s) 98, 761, 856, 1141
BpmI CTGGAG 2 cut(s) 950, 975
Bpu10I CCTNAGC 1 cut(s) 902
Bpu1102I GCTNAGC 1 cut(s) 437
BpuEI CTTGAG 2 cut(s) 749, 986
Bsa29I ATCGAT 1 cut(s) 57
BsaAI YACGTR 1 cut(s) 707
BsaHI GRCGYC 2 cut(s) 806, 1028
BsaJI CCNNGG 3 cut(s) 121, 643, 1049
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 560, 590
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 347, 649, 1615
Bse118I RCCGGY 1 cut(s) 1608
Bse1I ACTGG 2 cut(s) 1371, 1695
Bse3DI GCAATG 1 cut(s) 1230
BseBI CCWGG 2 cut(s) 11, 645
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 3 cut(s) 121, 643, 1049
BseGI GGATG 5 cut(s) 577, 846, 1060, 1290, 1658
BseLI CCNNNNNNNGG 4 cut(s) 158, 347, 649, 1615
BseMI GCAATG 1 cut(s) 1230
BseMII CTCAG 5 cut(s) 963, 1110, 1148, 1367, 1496
BseNI ACTGG 2 cut(s) 1371, 1695
BseXI GCAGC 5 cut(s) 34, 688, 733, 736, 745
BsgI GTGCAG 1 cut(s) 687
BshFI GGCC 1 cut(s) 492
BshVI ATCGAT 1 cut(s) 57
BsiSI CCGG 2 cut(s) 203, 1609
BslI CCNNNNNNNGG 4 cut(s) 158, 347, 649, 1615
BsmAI GTCTC 3 cut(s) 962, 1058, 1081
BsnI GGCC 1 cut(s) 492
Bsp1407I TGTACA 2 cut(s) 1282, 1525
Bsp1720I GCTNAGC 1 cut(s) 437
Bsp19I CCATGG 1 cut(s) 1049
BspACI CCGC 5 cut(s) 433, 583, 838, 869, 1031
BspANI GGCC 1 cut(s) 492
BspCNI CTCAG 5 cut(s) 964, 1111, 1149, 1366, 1497
BspDI ATCGAT 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 1260
BspMAI CTGCAG 1 cut(s) 763
BspMI ACCTGC 2 cut(s) 1570, 1704
BspPI GGATC 3 cut(s) 517, 521, 1365
BspTI CTTAAG 1 cut(s) 494
BsrDI GCAATG 1 cut(s) 1230
BsrFI RCCGGY 1 cut(s) 1608
BsrGI TGTACA 2 cut(s) 1282, 1525
BsrI ACTGG 2 cut(s) 1371, 1695
BssAI RCCGGY 1 cut(s) 1608
BssECI CCNNGG 3 cut(s) 121, 643, 1049
BssNI GRCGYC 2 cut(s) 806, 1028
BssT1I CCWWGG 1 cut(s) 1049
Bst2UI CCWGG 2 cut(s) 11, 645
Bst4CI ACNGT 4 cut(s) 170, 664, 789, 1069
Bst6I CTCTTC 7 cut(s) 223, 278, 537, 867, 1084, 1531, 1651
BstACI GRCGYC 2 cut(s) 806, 1028
BstAFI CTTAAG 1 cut(s) 494
BstAPI GCANNNNNTGC 1 cut(s) 758
BstAUI TGTACA 2 cut(s) 1282, 1525
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 1 cut(s) 634
BstDEI CTNAG 9 cut(s) 437, 599, 902, 972, 1119, 1157, 1242, 1353, 1505
BstDSI CCRYGG 1 cut(s) 1049
BstF5I GGATG 5 cut(s) 577, 846, 1060, 1290, 1658
BstHHI GCGC 2 cut(s) 89, 394
BstMAI GTCTC 3 cut(s) 962, 1058, 1081
BstNI CCWGG 2 cut(s) 11, 645
BstNSI RCATGY 2 cut(s) 636, 1429
BstSCI CCNGG 2 cut(s) 9, 643
BstSFI CTRYAG 2 cut(s) 759, 949
BstV1I GCAGC 5 cut(s) 34, 688, 733, 736, 745
BstX2I RGATCY 5 cut(s) 213, 230, 1092, 1357, 1628
BstYI RGATCY 5 cut(s) 213, 230, 1092, 1357, 1628
Bsu15I ATCGAT 1 cut(s) 57
BsuRI GGCC 1 cut(s) 492
BsuTUI ATCGAT 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 1049
BtrI CACGTC 1 cut(s) 305
BtsCI GGATG 5 cut(s) 577, 846, 1060, 1290, 1658
BtsIMutI CAGTG 2 cut(s) 175, 1688
BveI ACCTGC 2 cut(s) 1570, 1704
Cac8I GCNNGC 1 cut(s) 634
CaiI CAGNNNCTG 1 cut(s) 1360
CfoI GCGC 2 cut(s) 89, 394
Cfr10I RCCGGY 1 cut(s) 1608
Cfr13I GGNCC 2 cut(s) 1258, 1693
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 2 cut(s) 795, 1036
Csp6I GTAC 5 cut(s) 333, 653, 1283, 1526, 1583
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviQI GTAC 5 cut(s) 333, 653, 1283, 1526, 1583
DdeI CTNAG 9 cut(s) 437, 599, 902, 972, 1119, 1157, 1242, 1353, 1505
DraI TTTAAA 2 cut(s) 34, 418
DraIII CACNNNGTG 1 cut(s) 1382
Eam1104I CTCTTC 7 cut(s) 223, 278, 537, 867, 1084, 1531, 1651
EarI CTCTTC 7 cut(s) 223, 278, 537, 867, 1084, 1531, 1651
Eco130I CCWWGG 1 cut(s) 1049
Eco147I AGGCCT 1 cut(s) 492
Eco47I GGWCC 2 cut(s) 1258, 1693
Eco57I CTGAAG 1 cut(s) 354
Eco72I CACGTG 1 cut(s) 707
EcoO109I RGGNCCY 1 cut(s) 1258
EcoRII CCWGG 2 cut(s) 9, 643
EcoT14I CCWWGG 1 cut(s) 1049
EcoT22I ATGCAT 1 cut(s) 1405
ErhI CCWWGG 1 cut(s) 1049
FauNDI CATATG 1 cut(s) 514
FbaI TGATCA 1 cut(s) 1126
FblI GTMKAC 2 cut(s) 301, 1430
Fnu4HI GCNGC 7 cut(s) 48, 434, 702, 747, 750, 759, 1031
FokI GGATG 5 cut(s) 584, 853, 1067, 1277, 1645
Fsp4HI GCNGC 7 cut(s) 48, 434, 702, 747, 750, 759, 1031
FspBI CTAG 6 cut(s) 330, 825, 1211, 1224, 1623, 1650
FspI TGCGCA 2 cut(s) 88, 393
GlaI GCGC 2 cut(s) 88, 393
GluI GCNGC 7 cut(s) 48, 434, 702, 747, 750, 759, 1031
GsuI CTGGAG 2 cut(s) 950, 975
HaeIII GGCC 1 cut(s) 492
HapII CCGG 2 cut(s) 203, 1609
HgaI GACGC 2 cut(s) 795, 1036
HhaI GCGC 2 cut(s) 89, 394
Hin1I GRCGYC 2 cut(s) 806, 1028
Hin6I GCGC 2 cut(s) 87, 392
HinP1I GCGC 2 cut(s) 87, 392
HindIII AAGCTT 2 cut(s) 412, 1247
HinfI GANTC 4 cut(s) 196, 257, 995, 1643
HpaII CCGG 2 cut(s) 203, 1609
HphI GGTGA 1 cut(s) 1202
Hpy166II GTNNAC 3 cut(s) 302, 541, 1431
Hpy188I TCNGA 6 cut(s) 676, 1107, 1342, 1362, 1390, 1569
Hpy8I GTNNAC 3 cut(s) 302, 541, 1431
Hpy99I CGWCG 1 cut(s) 811
HpyAV CCTTC 5 cut(s) 351, 928, 1181, 1347, 1665
HpyCH4III ACNGT 4 cut(s) 170, 664, 789, 1069
HpyCH4IV ACGT 2 cut(s) 304, 706
HpyF3I CTNAG 9 cut(s) 437, 599, 902, 972, 1119, 1157, 1242, 1353, 1505
HpySE526I ACGT 2 cut(s) 304, 706
Hsp92I GRCGYC 2 cut(s) 806, 1028
HspAI GCGC 2 cut(s) 87, 392
Ksp22I TGATCA 1 cut(s) 1126
Lsp1109I GCAGC 5 cut(s) 34, 688, 733, 736, 745
LweI GCATC 4 cut(s) 98, 761, 856, 1141
MaeI CTAG 6 cut(s) 330, 825, 1211, 1224, 1623, 1650
MaeII ACGT 2 cut(s) 304, 706
MaeIII GTNAC 3 cut(s) 106, 1300, 1421
MflI RGATCY 5 cut(s) 213, 230, 1092, 1357, 1628
MluCI AATT 3 cut(s) 261, 894, 1447
MlyI GAGTC 1 cut(s) 1637
MmeI TCCRAC 2 cut(s) 294, 328
Mph1103I ATGCAT 1 cut(s) 1405
MroXI GAANNNNTTC 1 cut(s) 1447
MslI CAYNNNNRTG 1 cut(s) 1284
MspA1I CMGCKG 1 cut(s) 838
MspCI CTTAAG 1 cut(s) 494
MspI CCGG 2 cut(s) 203, 1609
MspR9I CCNGG 2 cut(s) 11, 645
MvaI CCWGG 2 cut(s) 11, 645
NcoI CCATGG 1 cut(s) 1049
NdeI CATATG 1 cut(s) 514
NlaIV GGNNCC 1 cut(s) 1260
NmuCI GTSAC 1 cut(s) 1300
NsbI TGCGCA 2 cut(s) 88, 393
NsiI ATGCAT 1 cut(s) 1405
NspI RCATGY 2 cut(s) 636, 1429
PaeI GCATGC 1 cut(s) 636
PceI AGGCCT 1 cut(s) 492
PciI ACATGT 1 cut(s) 1425
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 703
PdmI GAANNNNTTC 1 cut(s) 1447
PfeI GAWTC 3 cut(s) 196, 257, 995
PkrI GCNGC 7 cut(s) 49, 435, 703, 748, 751, 760, 1032
PleI GAGTC 1 cut(s) 1637
PmaCI CACGTG 1 cut(s) 707
PmlI CACGTG 1 cut(s) 707
PpsI GAGTC 1 cut(s) 1637
Ppu21I YACGTR 1 cut(s) 707
PpuMI RGGWCCY 1 cut(s) 1258
PscI ACATGT 1 cut(s) 1425
Psp5II RGGWCCY 1 cut(s) 1258
Psp6I CCWGG 2 cut(s) 9, 643
PspCI CACGTG 1 cut(s) 707
PspGI CCWGG 2 cut(s) 9, 643
PspN4I GGNNCC 1 cut(s) 1260
PspPI GGNCC 2 cut(s) 1258, 1693
PspPPI RGGWCCY 1 cut(s) 1258
PstI CTGCAG 1 cut(s) 763
PstNI CAGNNNCTG 1 cut(s) 1360
PsuI RGATCY 5 cut(s) 213, 230, 1092, 1357, 1628
RsaI GTAC 5 cut(s) 334, 654, 1284, 1527, 1584
RsaNI GTAC 5 cut(s) 333, 653, 1283, 1526, 1583
RseI CAYNNNNRTG 1 cut(s) 1284
SatI GCNGC 7 cut(s) 48, 434, 702, 747, 750, 759, 1031
Sau96I GGNCC 2 cut(s) 1258, 1693
ScaI AGTACT 1 cut(s) 334
SchI GAGTC 1 cut(s) 1637
ScrFI CCNGG 2 cut(s) 11, 645
SfaNI GCATC 4 cut(s) 98, 761, 856, 1141
SfcI CTRYAG 2 cut(s) 759, 949
SinI GGWCC 2 cut(s) 1258, 1693
SmiMI CAYNNNNRTG 1 cut(s) 1284
SmlI CTYRAG 3 cut(s) 494, 728, 965
SmoI CTYRAG 3 cut(s) 494, 728, 965
SpeI ACTAGT 1 cut(s) 1210
SphI GCATGC 1 cut(s) 636
Sse9I AATT 3 cut(s) 261, 894, 1447
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 5 cut(s) 433, 583, 838, 869, 1031
SspMI CTAG 6 cut(s) 330, 825, 1211, 1224, 1623, 1650
StuI AGGCCT 1 cut(s) 492
StyD4I CCNGG 2 cut(s) 9, 643
StyI CCWWGG 1 cut(s) 1049
TaaI ACNGT 4 cut(s) 170, 664, 789, 1069
TaiI ACGT 2 cut(s) 307, 709
TaqI TCGA 5 cut(s) 57, 113, 378, 809, 1014
TasI AATT 3 cut(s) 261, 894, 1447
TatI WGTACW 3 cut(s) 332, 1282, 1525
TauI GCSGC 2 cut(s) 436, 1033
TfiI GAWTC 3 cut(s) 196, 257, 995
TscAI CASTG 2 cut(s) 175, 1695
TseFI GTSAC 1 cut(s) 1300
TseI GCWGC 5 cut(s) 47, 701, 746, 749, 758
Tsp45I GTSAC 1 cut(s) 1300
TspDTI ATGAA 4 cut(s) 534, 1323, 1485, 1647
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 2 cut(s) 175, 1695
Vha464I CTTAAG 1 cut(s) 494
VpaK11BI GGWCC 2 cut(s) 1258, 1693
XapI RAATTY 2 cut(s) 894, 1447
XceI RCATGY 2 cut(s) 636, 1429
XmiI GTMKAC 2 cut(s) 301, 1430
XmnI GAANNNNTTC 1 cut(s) 1447
XspI CTAG 6 cut(s) 330, 825, 1211, 1224, 1623, 1650
ZrmI AGTACT 1 cut(s) 334
Zsp2I ATGCAT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.