MD04G1008700.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005611
Physical Location & Seq
Forward (+)
990447 .. 999845
9399 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1008700.v1.1.491

Sequence Viewer

Length: 2184 bp
ATGGCATGCCAAATGATACATTCGTGGAAATTTAACGAGCTAGTCGGTGCATTTCTTGATCTTGCAATAGCCTATCTTCTACTATGTGCCGCGGCAGTTGCCTACTTTACCTCGAAATTTGTTGGTCTGTTTGGGTTTTGTCTGCCGTGCCCTTGTGATGGGTTTTTTGGGACCCCAAGGAAAAGTGACTGCTTTCAGAGGCAATTTGCGGATCCCCCGTGTGAGAAAATCTCTTCAGTTCTATGGTCGGTTAGGAGCAAGTTCCCTCTCAATGTTATATGGGATGAAGACCCAAATTTCCATTCAAAGTTGAAGTCCCTAAATGAGGCAAATGGGAGTTTTGCGTTTGAAGGTGAAGCGTCATGTAGCTCATTTTCGGTGGCTGAGAATGAACACAGTGTTGAGTCTGGTGTAGGCAACTTGGCTGCTGGGAAGGAACAGAGTTTTGATCTTAAGGCAAAGAAGGTTGCAAGTCGAAGGCCGAGACTTCGGCGCCGCCGCAGAGGGGGCTCTGTTGATTATGGAAATTCATCATCAGTTTCATCATATGATATGTTCCAGTCCGCTGCACAGGACAATCCTACATCTCCTTCCAGCATTAGTAAAATGGGAAATGAGGTTACTGAAGTTTTTGTAAATTCAGATGGCATCGAAGCTCCTACAGATATGAGTTCGCCGGAAAGCGTTTCACGTGTTGGGTTTAATGAACATGTTGATGAAATAAAATCAGTGGAGAAGGATGGATCACTTGTTGAAGATTCTGGATCCAATGCAGGAGAGAAACTAGGTTTTGACAGTAATGAGACAACTACAATTAGAGTACTGGAACAGGCACTCGAAGAAGAGCATGCTGCTCGTGCTGCTCTGTATCTTGAACTGGAGAAGGAGAGAAGTGCCGCTGCCACCGCTGCAGATGAGGCCATGGCCATGATAATGCGCCTGCAAGAGGAGAAGGCATCAATAGAAATGGAGGCAAGGCAGTACCAAAGGATGATAGAGGAAAAATTTGCTTACGATGCTGAGGAAATGAACATTCTTAAAGAGATCTTAATTAGGAGGGAGAGGGAAAAGCATTTCTTGGAGAACGAAGTTGAGCAATACAGGCAAATGTTTTTTGGAGATGATCGAGTGGATTCAAGTGAGGACCAGGTACCAAAGCTGGAATTGGAACATAGTTCTCCAGATTATGCGGTCCCATCTACCAAAATAAAAGATTATACTCTTACTTTTGGTAAAGAACTACCAATTCTGGATTTGGATGAAGATTCTGACTCTTTGAAGCGGGTGGACATGCATTTGCATCCAAGCATAGAGAGTCATTCACATTTCTCAAGTAGTTCCAATAAGTTCAGTCACGAGTTTCAAGAAAAGGAAATAGTGTCTATGGATGAGAAACCTTCTTCTCAAGGAAAAGAGCTCCAGAAGCTAGAGGTATATCAACAACGCAGCCTGTCACCTAGTCCCGGGGGACTCAGTTTCCGTAAAAGTACTAGCCCTCCGTATAAGCAGGAACTGAATTTAGATGCTAGCACTAGCATATGGGAAGGGTTGGCTTCAAATACATCTGGGACTGGCAATGAAAATAGGAATCCTATTCCATCCAATAATGATAAAAGAGACAAGCAAGGGAACAACCAGGATGAGGGCAGCAGAGATCCACGTAGCACAGTGTTGAATACAGAGCCTAGTGTTCTTGATGTTCATGTTATTGATGATAAGTCTCATCTGTCCAATGCAGAGAAAAGTGAACGCGATATGCCAACCACGATTGGGTTGAAAAATCAGCGCAAAATCCAGAGAGTTAGTTCAGACACGTCTAATGTATTTCTCACAATGGATTGCCCACGAGGCAGATCTTTACCTTCTGATATGCGGAGATATTCAATGTCTGCAATTGATTATGAAAGATATAAAATTGATAATGAAGTTGAGCAGCTTGGAGAAAGGTTAAGGATTGTGCAAGAGGGAAGAGAGAAGCTCAACTTCTCAGTGGGGCACAGAGACAGGGAAAGGATTCAGCTGCAACTTTTGGAGGATATTGCAAGCCATCTTAGAGAGATACGGCAGTTAACAGAACCTGGAAAAGCTGAGGGCCAGGCTGCAATGCTGCCTCCATTATCTAAGGTCATGTCCAAGAAAAGACGTTGGCGGACTTTACCTCTACAAGTGCATACAAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

728

Amino Acids

81.91

Weight (kDa)

5.31

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 275 - 364 5.1e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1150
AccB1I GGYRCC 2 cut(s) 492, 1150
AccII CGCG 2 cut(s) 92, 1752
AclWI GGATC 6 cut(s) 206, 219, 751, 759, 772, 1649
AcoI YGGCCR 1 cut(s) 924
AcsI RAATTY 7 cut(s) 29, 116, 295, 526, 637, 1004, 1516
AcuI CTGAAG 2 cut(s) 219, 645
AcvI CACGTG 1 cut(s) 692
AcyI GRCGYC 1 cut(s) 493
AfaI GTAC 4 cut(s) 822, 983, 1152, 1489
AfiI CCNNNNNNNGG 7 cut(s) 158, 325, 505, 946, 1463, 1642, 1770
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 3 cut(s) 691, 709, 1812
AjiI CACGTC 1 cut(s) 1815
AjnI CCWGG 4 cut(s) 1146, 1635, 2077, 2094
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
Alw21I GWGCWC 1 cut(s) 1419
Alw26I GTCTC 5 cut(s) 478, 797, 1611, 1725, 1995
AlwI GGATC 6 cut(s) 206, 219, 751, 759, 772, 1649
AlwNI CAGNNNCTG 2 cut(s) 1513, 2078
Ama87I CYCGRG 1 cut(s) 1463
AoxI GGCC 4 cut(s) 479, 918, 924, 2092
ApoI RAATTY 7 cut(s) 29, 116, 295, 526, 637, 1004, 1516
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 1 cut(s) 2013
Asp718I GGTACC 1 cut(s) 1150
AspLEI GCGC 3 cut(s) 495, 939, 1788
AspS9I GGNCC 4 cut(s) 171, 1144, 1192, 2092
AsuC2I CCSGG 2 cut(s) 1464, 1465
AsuHPI GGTGA 2 cut(s) 365, 1446
AsuNHI GCTAGC 1 cut(s) 1526
AvaI CYCGRG 1 cut(s) 1463
AvaII GGWCC 3 cut(s) 171, 1144, 1192
BaeGI GKGCMC 2 cut(s) 152, 1998
BaeI ACNNNNGTAYC 4 cut(s) 8, 41, 2051, 2084
BalI TGGCCA 1 cut(s) 926
BamHI GGATCC 2 cut(s) 211, 764
BanI GGYRCC 2 cut(s) 492, 1150
BanII GRGCYC 2 cut(s) 512, 1419
BauI CACGAG 3 cut(s) 855, 1355, 1845
BbrPI CACGTG 1 cut(s) 692
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 1419
BbvCI CCTCAGC 2 cut(s) 1020, 2088
BccI CCATC 6 cut(s) 152, 638, 734, 1204, 1606, 2055
BceAI ACGGC 2 cut(s) 130, 2078
BcgI CGANNNNNNTGC 2 cut(s) 836, 870
BciT130I CCWGG 4 cut(s) 1148, 1637, 2079, 2096
BcnI CCSGG 2 cut(s) 1464, 1465
BcoDI GTCTC 5 cut(s) 478, 797, 1611, 1725, 1995
BfaI CTAG 8 cut(s) 41, 785, 1427, 1458, 1491, 1527, 1533, 1686
BfmI CTRYAG 2 cut(s) 660, 909
BfoI RGCGCY 1 cut(s) 496
BfrI CTTAAG 1 cut(s) 452
BglI GCCNNNNNGGC 1 cut(s) 1848
BglII AGATCT 2 cut(s) 1044, 1853
BmcAI AGTACT 2 cut(s) 822, 1489
Bme1390I CCNGG 6 cut(s) 1148, 1464, 1465, 1637, 2079, 2096
Bme18I GGWCC 3 cut(s) 171, 1144, 1192
BmeT110I CYCGRG 1 cut(s) 1463
BmgBI CACGTC 1 cut(s) 1815
BmgT120I GGNCC 4 cut(s) 171, 1144, 1192, 2092
BmiI GGNNCC 7 cut(s) 172, 173, 213, 494, 766, 1152, 1194
BmrFI CCNGG 6 cut(s) 1148, 1464, 1465, 1637, 2079, 2096
BmsI GCATC 5 cut(s) 657, 965, 1006, 1309, 1513
BmtI GCTAGC 1 cut(s) 1530
BpiI GAAGAC 1 cut(s) 294
BplI GAGNNNNNCTC 4 cut(s) 215, 247, 1962, 1994
BpmI CTGGAG 3 cut(s) 899, 1164, 1403
Bpu10I CCTNAGC 2 cut(s) 1020, 2088
BpuEI CTTGAG 2 cut(s) 1315, 1389
BpuMI CCSGG 2 cut(s) 1464, 1465
BsaAI YACGTR 2 cut(s) 692, 1661
BsaHI GRCGYC 1 cut(s) 493
BsaJI CCNNGG 5 cut(s) 90, 176, 921, 1463, 1464
BsaXI ACNNNNNCTCC 2 cut(s) 1480, 1510
Bsc4I CCNNNNNNNGG 7 cut(s) 158, 325, 505, 946, 1463, 1642, 1770
Bse1I ACTGG 4 cut(s) 559, 828, 882, 1576
Bse3DI GCAATG 2 cut(s) 1582, 2109
BseBI CCWGG 4 cut(s) 1148, 1637, 2079, 2096
BseDI CCNNGG 5 cut(s) 90, 176, 921, 1463, 1464
BseGI GGATG 8 cut(s) 289, 745, 996, 1264, 1300, 1393, 1598, 1645
BseLI CCNNNNNNNGG 7 cut(s) 158, 325, 505, 946, 1463, 1642, 1770
BseMI GCAATG 2 cut(s) 1582, 2109
BseMII CTCAG 5 cut(s) 375, 1011, 1486, 2001, 2079
BseNI ACTGG 4 cut(s) 559, 828, 882, 1576
BseRI GAGGAG 1 cut(s) 962
BseSI GKGCMC 2 cut(s) 152, 1998
BseYI CCCAGC 1 cut(s) 428
BsgI GTGCAG 1 cut(s) 552
Bsh1236I CGCG 2 cut(s) 92, 1752
BshFI GGCC 4 cut(s) 481, 920, 926, 2094
BshNI GGYRCC 2 cut(s) 492, 1150
BsiHKAI GWGCWC 1 cut(s) 1419
BsiHKCI CYCGRG 1 cut(s) 1463
BsiSI CCGG 2 cut(s) 677, 1464
BslFI GGGAC 6 cut(s) 184, 301, 1178, 1446, 1482, 1582
BslI CCNNNNNNNGG 7 cut(s) 158, 325, 505, 946, 1463, 1642, 1770
BsmAI GTCTC 5 cut(s) 478, 797, 1611, 1725, 1995
BsmFI GGGAC 6 cut(s) 184, 301, 1178, 1446, 1482, 1582
BsnI GGCC 4 cut(s) 481, 920, 926, 2094
BsoBI CYCGRG 1 cut(s) 1463
Bsp1286I GDGCHC 4 cut(s) 152, 512, 1419, 1998
Bsp143I GATC 9 cut(s) 58, 211, 448, 743, 764, 1044, 1123, 1654, 1853
Bsp19I CCATGG 1 cut(s) 921
BspANI GGCC 4 cut(s) 481, 920, 926, 2094
BspCNI CTCAG 5 cut(s) 376, 1012, 1485, 2000, 2080
BspFNI CGCG 2 cut(s) 92, 1752
BspLI GGNNCC 7 cut(s) 172, 173, 213, 494, 766, 1152, 1194
BspMAI CTGCAG 1 cut(s) 913
BspOI GCTAGC 1 cut(s) 1530
BspPI GGATC 6 cut(s) 206, 219, 751, 759, 772, 1649
BspQI GCTCTTC 1 cut(s) 837
BspT107I GGYRCC 2 cut(s) 492, 1150
BspTI CTTAAG 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 1582, 2109
BsrI ACTGG 4 cut(s) 559, 828, 882, 1576
BssECI CCNNGG 5 cut(s) 90, 176, 921, 1463, 1464
BssMI GATC 9 cut(s) 58, 211, 448, 743, 764, 1044, 1123, 1654, 1853
BssNI GRCGYC 1 cut(s) 493
BssSI CACGAG 3 cut(s) 855, 1355, 1845
BssT1I CCWWGG 2 cut(s) 176, 921
Bst2BI CACGAG 3 cut(s) 855, 1355, 1845
Bst2UI CCWGG 4 cut(s) 1148, 1637, 2079, 2096
Bst4CI ACNGT 3 cut(s) 398, 797, 1669
Bst6I CTCTTC 3 cut(s) 238, 837, 1963
BstACI GRCGYC 1 cut(s) 493
BstAFI CTTAAG 1 cut(s) 452
BstBAI YACGTR 2 cut(s) 692, 1661
BstC8I GCNNGC 5 cut(s) 7, 849, 941, 1528, 2044
BstDEI CTNAG 7 cut(s) 384, 1020, 1472, 1987, 2051, 2088, 2121
BstDSI CCRYGG 2 cut(s) 90, 921
BstENI CCTNNNNNAGG 2 cut(s) 323, 944
BstF5I GGATG 8 cut(s) 289, 745, 996, 1264, 1300, 1393, 1598, 1645
BstFNI CGCG 2 cut(s) 92, 1752
BstH2I RGCGCY 1 cut(s) 496
BstHHI GCGC 3 cut(s) 495, 939, 1788
BstKTI GATC 9 cut(s) 61, 214, 451, 746, 767, 1047, 1126, 1657, 1856
BstMAI GTCTC 5 cut(s) 478, 797, 1611, 1725, 1995
BstMBI GATC 9 cut(s) 58, 211, 448, 743, 764, 1044, 1123, 1654, 1853
BstNI CCWGG 4 cut(s) 1148, 1637, 2079, 2096
BstNSI RCATGY 4 cut(s) 9, 713, 851, 1294
BstSCI CCNGG 6 cut(s) 1146, 1462, 1463, 1635, 2077, 2094
BstSFI CTRYAG 2 cut(s) 660, 909
BstSLI GKGCMC 2 cut(s) 152, 1998
BstUI CGCG 2 cut(s) 92, 1752
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 5 cut(s) 211, 764, 1044, 1654, 1853
BstYI RGATCY 5 cut(s) 211, 764, 1044, 1654, 1853
BsuRI GGCC 4 cut(s) 481, 920, 926, 2094
BtgI CCRYGG 2 cut(s) 90, 921
BtrI CACGTC 1 cut(s) 1815
BtsCI GGATG 8 cut(s) 289, 745, 996, 1264, 1300, 1393, 1598, 1645
BtsIMutI CAGTG 4 cut(s) 403, 735, 1674, 1995
Cac8I GCNNGC 5 cut(s) 7, 849, 941, 1528, 2044
CaiI CAGNNNCTG 2 cut(s) 1513, 2078
CfoI GCGC 3 cut(s) 495, 939, 1788
Cfr13I GGNCC 4 cut(s) 171, 1144, 1192, 2092
Cfr42I CCGCGG 1 cut(s) 93
Cfr9I CCCGGG 1 cut(s) 1463
CseI GACGC 1 cut(s) 348
CsiI ACCWGGT 1 cut(s) 1146
Csp6I GTAC 4 cut(s) 821, 982, 1151, 1488
CviAII CATG 9 cut(s) 6, 363, 710, 848, 922, 928, 1291, 1703, 2128
CviQI GTAC 4 cut(s) 821, 982, 1151, 1488
DdeI CTNAG 7 cut(s) 384, 1020, 1472, 1987, 2051, 2088, 2121
DinI GGCGCC 1 cut(s) 494
DpnI GATC 9 cut(s) 60, 213, 450, 745, 766, 1046, 1125, 1656, 1855
DpnII GATC 9 cut(s) 58, 211, 448, 743, 764, 1044, 1123, 1654, 1853
EaeI YGGCCR 1 cut(s) 924
Eam1104I CTCTTC 3 cut(s) 238, 837, 1963
EarI CTCTTC 3 cut(s) 238, 837, 1963
EciI GGCGGA 1 cut(s) 2164
Ecl136II GAGCTC 1 cut(s) 1417
Eco130I CCWWGG 2 cut(s) 176, 921
Eco24I GRGCYC 2 cut(s) 512, 1419
Eco47I GGWCC 3 cut(s) 171, 1144, 1192
Eco53kI GAGCTC 1 cut(s) 1417
Eco57I CTGAAG 2 cut(s) 219, 645
Eco72I CACGTG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 1463
EcoICRI GAGCTC 1 cut(s) 1417
EcoNI CCTNNNNNAGG 2 cut(s) 323, 944
EcoO109I RGGNCCY 1 cut(s) 171
EcoRII CCWGG 4 cut(s) 1146, 1635, 2077, 2094
EcoT14I CCWWGG 2 cut(s) 176, 921
EcoT22I ATGCAT 1 cut(s) 1296
EcoT38I GRGCYC 2 cut(s) 512, 1419
EgeI GGCGCC 1 cut(s) 494
EheI GGCGCC 1 cut(s) 494
ErhI CCWWGG 2 cut(s) 176, 921
FaeI CATG 9 cut(s) 9, 366, 713, 851, 925, 931, 1294, 1706, 2131
FalI AAGNNNNNCTT 4 cut(s) 1061, 1093, 1967, 1999
FaqI GGGAC 6 cut(s) 184, 301, 1178, 1446, 1482, 1582
FatI CATG 9 cut(s) 5, 362, 709, 847, 921, 927, 1290, 1702, 2127
FauI CCCGC 1 cut(s) 1275
FauNDI CATATG 2 cut(s) 547, 1538
FokI GGATG 8 cut(s) 296, 752, 1003, 1271, 1287, 1400, 1585, 1652
FriOI GRGCYC 2 cut(s) 512, 1419
FspBI CTAG 8 cut(s) 41, 785, 1427, 1458, 1491, 1527, 1533, 1686
GlaI GCGC 3 cut(s) 494, 938, 1787
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 3 cut(s) 899, 1164, 1403
HaeII RGCGCY 1 cut(s) 496
HaeIII GGCC 4 cut(s) 481, 920, 926, 2094
HapII CCGG 2 cut(s) 677, 1464
HgaI GACGC 1 cut(s) 348
HhaI GCGC 3 cut(s) 495, 939, 1788
Hin1I GRCGYC 1 cut(s) 493
Hin1II CATG 9 cut(s) 9, 366, 713, 851, 925, 931, 1294, 1706, 2131
Hin6I GCGC 3 cut(s) 493, 937, 1786
HinP1I GCGC 3 cut(s) 493, 937, 1786
HincII GTYRAC 1 cut(s) 2070
HindII GTYRAC 1 cut(s) 2070
HinfI GANTC 9 cut(s) 404, 758, 1133, 1265, 1271, 1315, 1470, 1588, 2014
HpaI GTTAAC 1 cut(s) 2070
HpaII CCGG 2 cut(s) 677, 1464
HphI GGTGA 2 cut(s) 365, 1446
Hpy166II GTNNAC 3 cut(s) 1288, 1748, 2070
Hpy188I TCNGA 5 cut(s) 198, 643, 1270, 1810, 1867
Hpy8I GTNNAC 3 cut(s) 1288, 1748, 2070
HpyCH4III ACNGT 3 cut(s) 398, 797, 1669
HpyCH4IV ACGT 4 cut(s) 691, 1660, 1814, 2143
HpyF3I CTNAG 7 cut(s) 384, 1020, 1472, 1987, 2051, 2088, 2121
HpySE526I ACGT 4 cut(s) 691, 1660, 1814, 2143
Hsp92I GRCGYC 1 cut(s) 493
Hsp92II CATG 9 cut(s) 9, 366, 713, 851, 925, 931, 1294, 1706, 2131
HspAI GCGC 3 cut(s) 493, 937, 1786
KasI GGCGCC 1 cut(s) 492
KflI GGGWCCC 1 cut(s) 171
KpnI GGTACC 1 cut(s) 1154
KspAI GTTAAC 1 cut(s) 2070
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 9 cut(s) 58, 211, 448, 743, 764, 1044, 1123, 1654, 1853
LguI GCTCTTC 1 cut(s) 837
LmnI GCTCC 3 cut(s) 255, 661, 1422
LweI GCATC 5 cut(s) 657, 965, 1006, 1309, 1513
MabI ACCWGGT 1 cut(s) 1146
MaeI CTAG 8 cut(s) 41, 785, 1427, 1458, 1491, 1527, 1533, 1686
MaeII ACGT 4 cut(s) 691, 1660, 1814, 2143
MaeIII GTNAC 4 cut(s) 185, 619, 1352, 1452
MalI GATC 9 cut(s) 60, 213, 450, 745, 766, 1046, 1125, 1656, 1855
MboI GATC 9 cut(s) 58, 211, 448, 743, 764, 1044, 1123, 1654, 1853
MboII GAAGA 9 cut(s) 68, 225, 299, 767, 851, 854, 1274, 1392, 1980
MfeI CAATTG 1 cut(s) 1893
MflI RGATCY 5 cut(s) 211, 764, 1044, 1654, 1853
MhlI GDGCHC 4 cut(s) 152, 512, 1419, 1998
MlsI TGGCCA 1 cut(s) 926
MluNI TGGCCA 1 cut(s) 926
Mly113I GGCGCC 1 cut(s) 493
MlyI GAGTC 4 cut(s) 413, 1265, 1324, 1464
Mox20I TGGCCA 1 cut(s) 926
Mph1103I ATGCAT 1 cut(s) 1296
MroXI GAANNNNTTC 1 cut(s) 2013
MscI TGGCCA 1 cut(s) 926
MseI TTAA 8 cut(s) 33, 453, 702, 1038, 1049, 1949, 2069, 2182
MslI CAYNNNNRTG 3 cut(s) 714, 926, 932
Msp20I TGGCCA 1 cut(s) 926
MspA1I CMGCKG 5 cut(s) 92, 566, 899, 908, 2020
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 2 cut(s) 677, 1464
MspR9I CCNGG 6 cut(s) 1148, 1464, 1465, 1637, 2079, 2096
MunI CAATTG 1 cut(s) 1893
MvaI CCWGG 4 cut(s) 1148, 1637, 2079, 2096
MvnI CGCG 2 cut(s) 92, 1752
NarI GGCGCC 1 cut(s) 493
NciI CCSGG 2 cut(s) 1464, 1465
NcoI CCATGG 1 cut(s) 921
NdeI CATATG 2 cut(s) 547, 1538
NdeII GATC 9 cut(s) 58, 211, 448, 743, 764, 1044, 1123, 1654, 1853
NheI GCTAGC 1 cut(s) 1526
NlaIII CATG 9 cut(s) 9, 366, 713, 851, 925, 931, 1294, 1706, 2131
NlaIV GGNNCC 7 cut(s) 172, 173, 213, 494, 766, 1152, 1194
NmeAIII GCCGAG 1 cut(s) 507
NmuCI GTSAC 3 cut(s) 185, 1352, 1452
NsiI ATGCAT 1 cut(s) 1296
NspI RCATGY 4 cut(s) 9, 713, 851, 1294
PaeI GCATGC 2 cut(s) 9, 851
PciI ACATGT 1 cut(s) 709
PciSI GCTCTTC 1 cut(s) 837
PdmI GAANNNNTTC 1 cut(s) 2013
PfeI GAWTC 5 cut(s) 758, 1133, 1265, 1588, 2014
PleI GAGTC 4 cut(s) 412, 1265, 1323, 1464
PluTI GGCGCC 1 cut(s) 496
PmaCI CACGTG 1 cut(s) 692
PmlI CACGTG 1 cut(s) 692
PpsI GAGTC 4 cut(s) 412, 1265, 1323, 1464
Ppu21I YACGTR 2 cut(s) 692, 1661
PpuMI RGGWCCY 1 cut(s) 171
PscI ACATGT 1 cut(s) 709
Psp124BI GAGCTC 1 cut(s) 1419
Psp5II RGGWCCY 1 cut(s) 171
Psp6I CCWGG 4 cut(s) 1146, 1635, 2077, 2094
PspCI CACGTG 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 4 cut(s) 1146, 1635, 2077, 2094
PspN4I GGNNCC 7 cut(s) 172, 173, 213, 494, 766, 1152, 1194
PspPI GGNCC 4 cut(s) 171, 1144, 1192, 2092
PspPPI RGGWCCY 1 cut(s) 171
PstI CTGCAG 1 cut(s) 913
PstNI CAGNNNCTG 2 cut(s) 1513, 2078
PsuI RGATCY 5 cut(s) 211, 764, 1044, 1654, 1853
PvuII CAGCTG 1 cut(s) 2020
RsaI GTAC 4 cut(s) 822, 983, 1152, 1489
RsaNI GTAC 4 cut(s) 821, 982, 1151, 1488
RseI CAYNNNNRTG 3 cut(s) 714, 926, 932
SacI GAGCTC 1 cut(s) 1419
SacII CCGCGG 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 837
SaqAI TTAA 8 cut(s) 33, 453, 702, 1038, 1049, 1949, 2069, 2182
Sau3AI GATC 9 cut(s) 58, 211, 448, 743, 764, 1044, 1123, 1654, 1853
Sau96I GGNCC 4 cut(s) 171, 1144, 1192, 2092
ScaI AGTACT 2 cut(s) 822, 1489
SchI GAGTC 4 cut(s) 413, 1265, 1324, 1464
ScrFI CCNGG 6 cut(s) 1148, 1464, 1465, 1637, 2079, 2096
SduI GDGCHC 4 cut(s) 152, 512, 1419, 1998
SexAI ACCWGGT 1 cut(s) 1146
SfaNI GCATC 5 cut(s) 657, 965, 1006, 1309, 1513
SfcI CTRYAG 2 cut(s) 660, 909
SfoI GGCGCC 1 cut(s) 494
Sfr303I CCGCGG 1 cut(s) 93
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 3 cut(s) 171, 1144, 1192
SmaI CCCGGG 1 cut(s) 1465
SmiMI CAYNNNNRTG 3 cut(s) 714, 926, 932
SmlI CTYRAG 3 cut(s) 452, 1330, 1404
SmoI CTYRAG 3 cut(s) 452, 1330, 1404
SphI GCATGC 2 cut(s) 9, 851
SspDI GGCGCC 1 cut(s) 492
SspMI CTAG 8 cut(s) 41, 785, 1427, 1458, 1491, 1527, 1533, 1686
SstI GAGCTC 1 cut(s) 1419
StyD4I CCNGG 6 cut(s) 1146, 1462, 1463, 1635, 2077, 2094
StyI CCWWGG 2 cut(s) 176, 921
TaaI ACNGT 3 cut(s) 398, 797, 1669
TaiI ACGT 4 cut(s) 694, 1663, 1817, 2146
TaqI TCGA 5 cut(s) 113, 475, 651, 837, 1126
TatI WGTACW 2 cut(s) 820, 1487
TauI GCSGC 5 cut(s) 92, 95, 498, 501, 899
TfiI GAWTC 5 cut(s) 758, 1133, 1265, 1588, 2014
Tru1I TTAA 8 cut(s) 33, 453, 702, 1038, 1049, 1949, 2069, 2182
Tru9I TTAA 8 cut(s) 33, 453, 702, 1038, 1049, 1949, 2069, 2182
TscAI CASTG 4 cut(s) 403, 735, 1674, 1995
TseFI GTSAC 3 cut(s) 185, 1352, 1452
Tsp45I GTSAC 3 cut(s) 185, 1352, 1452
TspGWI ACGGA 2 cut(s) 1469, 1488
TspMI CCCGGG 1 cut(s) 1463
TspRI CASTG 4 cut(s) 403, 735, 1674, 1995
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 3 cut(s) 171, 1144, 1192
XagI CCTNNNNNAGG 2 cut(s) 323, 944
XapI RAATTY 7 cut(s) 29, 116, 295, 526, 637, 1004, 1516
XceI RCATGY 4 cut(s) 9, 713, 851, 1294
XmaI CCCGGG 1 cut(s) 1463
XmnI GAANNNNTTC 1 cut(s) 2013
XspI CTAG 8 cut(s) 41, 785, 1427, 1458, 1491, 1527, 1533, 1686
ZrmI AGTACT 2 cut(s) 822, 1489
Zsp2I ATGCAT 1 cut(s) 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.