MD13G1285200.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005611
Physical Location & Seq
Forward (+)
43982577 .. 43984743
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1285200.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGCCCACGATAATTGGTCAGGAAGATCCTTTGCATTAGCTCAGGAGAGGATCCAAATCCGTGCATTTCTTAATCTTGCAATAGCCTATCTTCTACTGTGTGCGGCAGCGGTTGCCTATTTCACCACGAAATTTGTGGGTTTGTTTGGGTTGTGTCTGCCTTGCCCTTGTGATGGGTTTTCGGGACCTCAAGGAAAAGTAACTGCTTTCAGATCATCTACCGTGCCGAGACTTCGGCGCGGCCGCAGAGGGGGCTCTGTTGATAATGGAAAGTCGTCATCAGTTTTATCATATGATATGGTCCAGTCAGCGGCACAGGACATTCCTACATCTCCTTCCAGCGTTAGTAAAATGGGGAATGAGGTTTCGGTTACTGAAGTTTTTGTAAATTCAGATGGCATCGAAGCTTCTACAGATACGAGTTCGTCGGAAAGCGTTTCACGTGTTGAGTTGAATGAACATGTTGATGAAACTAAACCAATGGATGGATTACTTGTTGAAGAATCTGGATCCAACGCAGGAGAGAAACTAGGTTTTGACAGTAATGAGACAACTACGATTAGAGTACTGGAACAGGCACTCAAAGAAGAACATGCTGCTCGTGCTGCTCTGTATCTTGAACTAGAGGAGGAGAGAAGTGCTGCTGTCACCGCTGCAGATGAGGCCATGGCCATGATATTGCGCCTGCAAGAGGAGAAGGCATCAATTGAAATGGAGGCAAGGCAATACCAAAGGATGATAGAGGAAAAATCGGCTTACGATGTTGTGGAAATGAACATTCTTAAAGAGATGTCAGTTAGGAGGGAGAGGGAAAAGCATTTCTTGGAGAAGGAAATTGAGCGATACTGGCAAATGGTTTTTGGAGATGGTCATGTGGATTCAACTGAGAAGCCAACGCTGGAATTGGGACATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

306

Amino Acids

33.75

Weight (kDa)

4.92

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 190 - 279 2.2e-30 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 485
AccII CGCG 1 cut(s) 240
AciI CCGC 6 cut(s) 104, 110, 240, 244, 311, 651
AclWI GGATC 5 cut(s) 21, 46, 59, 504, 517
AcoI YGGCCR 2 cut(s) 241, 669
AcsI RAATTY 2 cut(s) 131, 388
AcuI CTGAAG 1 cut(s) 396
AcvI CACGTG 1 cut(s) 443
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 5 cut(s) 173, 250, 310, 485, 691
AflIII ACRYGT 2 cut(s) 442, 460
AgsI TTSAA 5 cut(s) 454, 500, 620, 710, 882
AluBI AGCT 2 cut(s) 41, 407
AluI AGCT 2 cut(s) 41, 407
Alw26I GTCTC 2 cut(s) 223, 542
AlwI GGATC 5 cut(s) 21, 46, 59, 504, 517
AoxI GGCC 4 cut(s) 3, 241, 663, 669
ApeKI GCWGC 5 cut(s) 107, 596, 605, 641, 653
ApoI RAATTY 2 cut(s) 131, 388
AspLEI GCGC 2 cut(s) 240, 684
AspS9I GGNCC 3 cut(s) 4, 185, 301
AsuHPI GGTGA 2 cut(s) 115, 640
AvaII GGWCC 2 cut(s) 185, 301
BalI TGGCCA 1 cut(s) 671
BamHI GGATCC 2 cut(s) 51, 509
BanII GRGCYC 1 cut(s) 257
BauI CACGAG 1 cut(s) 600
BbrPI CACGTG 1 cut(s) 443
BbvI GCAGC 5 cut(s) 119, 583, 592, 628, 640
BccI CCATC 4 cut(s) 167, 389, 479, 860
BcoDI GTCTC 2 cut(s) 223, 542
BfaI CTAG 2 cut(s) 530, 623
BfmI CTRYAG 2 cut(s) 411, 654
BisI GCNGC 9 cut(s) 105, 108, 241, 244, 312, 597, 606, 642, 654
BlsI GCNGC 9 cut(s) 106, 109, 242, 245, 313, 598, 607, 643, 655
BmcAI AGTACT 1 cut(s) 567
Bme18I GGWCC 2 cut(s) 185, 301
BmgT120I GGNCC 3 cut(s) 4, 185, 301
BmiI GGNNCC 3 cut(s) 53, 186, 511
BmsI GCATC 2 cut(s) 408, 710
Bpu10I CCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 443
BsaBI GATNNNNATC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 666
Bsc4I CCNNNNNNNGG 5 cut(s) 173, 250, 310, 485, 691
Bse1I ACTGG 3 cut(s) 304, 573, 851
Bse8I GATNNNNATC 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 666
BseGI GGATG 2 cut(s) 490, 741
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 5 cut(s) 173, 250, 310, 485, 691
BseMII CTCAG 2 cut(s) 56, 876
BseNI ACTGG 3 cut(s) 304, 573, 851
BseRI GAGGAG 3 cut(s) 641, 644, 707
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 5 cut(s) 119, 583, 592, 628, 640
Bsh1236I CGCG 1 cut(s) 240
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 4 cut(s) 5, 243, 665, 671
BsiEI CGRYCG 1 cut(s) 244
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 5 cut(s) 173, 250, 310, 485, 691
BsmAI GTCTC 2 cut(s) 223, 542
BsmFI GGGAC 1 cut(s) 198
BsnI GGCC 4 cut(s) 5, 243, 665, 671
Bsp1286I GDGCHC 1 cut(s) 257
Bsp143I GATC 4 cut(s) 26, 51, 212, 509
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 6 cut(s) 104, 110, 240, 244, 311, 651
BspANI GGCC 4 cut(s) 5, 243, 665, 671
BspCNI CTCAG 2 cut(s) 55, 877
BspFNI CGCG 1 cut(s) 240
BspLI GGNNCC 3 cut(s) 53, 186, 511
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 5 cut(s) 21, 46, 59, 504, 517
BsrI ACTGG 3 cut(s) 304, 573, 851
BssECI CCNNGG 1 cut(s) 666
BssMI GATC 4 cut(s) 26, 51, 212, 509
BssSI CACGAG 1 cut(s) 600
BssT1I CCWWGG 1 cut(s) 666
Bst2BI CACGAG 1 cut(s) 600
Bst4CI ACNGT 3 cut(s) 99, 223, 542
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 686
BstDEI CTNAG 2 cut(s) 42, 885
BstDSI CCRYGG 1 cut(s) 666
BstENI CCTNNNNNAGG 1 cut(s) 689
BstF5I GGATG 2 cut(s) 490, 741
BstFNI CGCG 1 cut(s) 240
BstHHI GCGC 2 cut(s) 240, 684
BstKTI GATC 4 cut(s) 29, 54, 215, 512
BstMAI GTCTC 2 cut(s) 223, 542
BstMBI GATC 4 cut(s) 26, 51, 212, 509
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 7 cut(s) 113, 252, 602, 605, 650, 662, 847
BstNSI RCATGY 2 cut(s) 464, 596
BstSFI CTRYAG 2 cut(s) 411, 654
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 5 cut(s) 119, 583, 592, 628, 640
BstX2I RGATCY 3 cut(s) 26, 51, 509
BstYI RGATCY 3 cut(s) 26, 51, 509
BstZI CGGCCG 1 cut(s) 241
BsuRI GGCC 4 cut(s) 5, 243, 665, 671
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 2 cut(s) 490, 741
Cac8I GCNNGC 1 cut(s) 686
CciNI GCGGCCGC 1 cut(s) 241
CfoI GCGC 2 cut(s) 240, 684
Cfr13I GGNCC 3 cut(s) 4, 185, 301
Csp6I GTAC 1 cut(s) 566
CspCI CAANNNNNGTGG 3 cut(s) 31, 115, 150
CviAII CATG 5 cut(s) 461, 593, 667, 673, 872
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 2 cut(s) 42, 885
DpnI GATC 4 cut(s) 28, 53, 214, 511
DpnII GATC 4 cut(s) 26, 51, 212, 509
EaeI YGGCCR 2 cut(s) 241, 669
EagI CGGCCG 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 666
Eco24I GRGCYC 1 cut(s) 257
Eco47I GGWCC 2 cut(s) 185, 301
Eco52I CGGCCG 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 396
Eco72I CACGTG 1 cut(s) 443
EcoNI CCTNNNNNAGG 1 cut(s) 689
EcoO109I RGGNCCY 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 5 cut(s) 464, 596, 670, 676, 875
FaiI YATR 9 cut(s) 292, 294, 299, 462, 594, 668, 674, 873, 912
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FaqI GGGAC 1 cut(s) 198
FatI CATG 5 cut(s) 460, 592, 666, 672, 871
FauNDI CATATG 1 cut(s) 292
Fnu4HI GCNGC 9 cut(s) 105, 108, 241, 244, 312, 597, 606, 642, 654
FokI GGATG 2 cut(s) 497, 748
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 9 cut(s) 105, 108, 241, 244, 312, 597, 606, 642, 654
FspBI CTAG 2 cut(s) 530, 623
GlaI GCGC 2 cut(s) 239, 683
GluI GCNGC 9 cut(s) 105, 108, 241, 244, 312, 597, 606, 642, 654
HaeIII GGCC 4 cut(s) 5, 243, 665, 671
HhaI GCGC 2 cut(s) 240, 684
Hin1II CATG 5 cut(s) 464, 596, 670, 676, 875
Hin6I GCGC 2 cut(s) 238, 682
HinP1I GCGC 2 cut(s) 238, 682
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 2 cut(s) 503, 878
HphI GGTGA 2 cut(s) 115, 640
Hpy188I TCNGA 3 cut(s) 212, 394, 430
Hpy188III TCNNGA 5 cut(s) 21, 44, 183, 507, 617
Hpy99I CGWCG 1 cut(s) 430
HpyAV CCTTC 3 cut(s) 345, 691, 823
HpyCH4III ACNGT 3 cut(s) 99, 223, 542
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 5 cut(s) 35, 65, 80, 656, 688
HpyF10VI GCNNNNNNNGC 7 cut(s) 113, 252, 602, 605, 650, 662, 847
HpyF3I CTNAG 2 cut(s) 42, 885
HpySE526I ACGT 1 cut(s) 442
Hsp92II CATG 5 cut(s) 464, 596, 670, 676, 875
HspAI GCGC 2 cut(s) 238, 682
Kzo9I GATC 4 cut(s) 26, 51, 212, 509
Lsp1109I GCAGC 5 cut(s) 119, 583, 592, 628, 640
LweI GCATC 2 cut(s) 408, 710
MaeI CTAG 2 cut(s) 530, 623
MaeII ACGT 1 cut(s) 442
MaeIII GTNAC 3 cut(s) 199, 370, 646
MalI GATC 4 cut(s) 28, 53, 214, 511
MboI GATC 4 cut(s) 26, 51, 212, 509
MboII GAAGA 4 cut(s) 36, 83, 512, 599
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 3 cut(s) 26, 51, 509
MhlI GDGCHC 1 cut(s) 257
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 6 cut(s) 13, 131, 388, 705, 834, 902
MluNI TGGCCA 1 cut(s) 671
MmeI TCCRAC 2 cut(s) 408, 537
Mox20I TGGCCA 1 cut(s) 671
MscI TGGCCA 1 cut(s) 671
MseI TTAA 3 cut(s) 72, 783, 916
MslI CAYNNNNRTG 2 cut(s) 465, 671
Msp20I TGGCCA 1 cut(s) 671
MspA1I CMGCKG 3 cut(s) 110, 311, 653
MunI CAATTG 1 cut(s) 705
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 7 cut(s) 113, 252, 602, 605, 650, 662, 847
NcoI CCATGG 1 cut(s) 666
NdeI CATATG 1 cut(s) 292
NdeII GATC 4 cut(s) 26, 51, 212, 509
NlaIII CATG 5 cut(s) 464, 596, 670, 676, 875
NlaIV GGNNCC 3 cut(s) 53, 186, 511
NmeAIII GCCGAG 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 646
NotI GCGGCCGC 1 cut(s) 241
NspI RCATGY 2 cut(s) 464, 596
PciI ACATGT 1 cut(s) 460
PfeI GAWTC 2 cut(s) 503, 878
PflMI CCANNNNNTGG 1 cut(s) 485
PkrI GCNGC 9 cut(s) 106, 109, 242, 245, 313, 598, 607, 643, 655
PmaCI CACGTG 1 cut(s) 443
PmlI CACGTG 1 cut(s) 443
Ppu21I YACGTR 1 cut(s) 443
PpuMI RGGWCCY 1 cut(s) 185
PscI ACATGT 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 185
PspCI CACGTG 1 cut(s) 443
PspN4I GGNNCC 3 cut(s) 53, 186, 511
PspPI GGNCC 3 cut(s) 4, 185, 301
PspPPI RGGWCCY 1 cut(s) 185
PstI CTGCAG 1 cut(s) 658
PsuI RGATCY 3 cut(s) 26, 51, 509
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
RseI CAYNNNNRTG 2 cut(s) 465, 671
SaqAI TTAA 3 cut(s) 72, 783, 916
SatI GCNGC 9 cut(s) 105, 108, 241, 244, 312, 597, 606, 642, 654
Sau3AI GATC 4 cut(s) 26, 51, 212, 509
Sau96I GGNCC 3 cut(s) 4, 185, 301
ScaI AGTACT 1 cut(s) 567
SduI GDGCHC 1 cut(s) 257
SetI ASST 6 cut(s) 43, 190, 366, 409, 445, 535
SfaNI GCATC 2 cut(s) 408, 710
SfcI CTRYAG 2 cut(s) 411, 654
SinI GGWCC 2 cut(s) 185, 301
SmiMI CAYNNNNRTG 2 cut(s) 465, 671
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
Sse9I AATT 6 cut(s) 13, 131, 388, 705, 834, 902
SsiI CCGC 6 cut(s) 104, 110, 240, 244, 311, 651
SspMI CTAG 2 cut(s) 530, 623
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 3 cut(s) 99, 223, 542
TaiI ACGT 1 cut(s) 445
TaqI TCGA 1 cut(s) 402
TasI AATT 6 cut(s) 13, 131, 388, 705, 834, 902
TatI WGTACW 1 cut(s) 565
TauI GCSGC 4 cut(s) 107, 243, 246, 314
TfiI GAWTC 2 cut(s) 503, 878
Tru1I TTAA 3 cut(s) 72, 783, 916
Tru9I TTAA 3 cut(s) 72, 783, 916
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 5 cut(s) 107, 596, 605, 641, 653
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 3 cut(s) 471, 483, 788
TspGWI ACGGA 1 cut(s) 50
Van91I CCANNNNNTGG 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 185, 301
XagI CCTNNNNNAGG 1 cut(s) 689
XapI RAATTY 2 cut(s) 131, 388
XceI RCATGY 2 cut(s) 464, 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XspI CTAG 2 cut(s) 530, 623
ZrmI AGTACT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.