RLG00000008078

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005611
Physical Location & Seq
Forward (+)
25359497 .. 25368049
8553 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008078

Sequence Viewer

Length: 2922 bp
ATGGCATGCCAAATGATACATTCATGGACATTCAGTGGGCTTGTGGGTGCATTTCTTGATCTTGCAATAGCCTATCTCTTACTCTGTGCGTCAGCTCTTGCATTTTTTACCTCGAAGTTTTTGAATTTCTTTGGATTAAGTCTTCCATGCCCTTGTGATGGGCTATTTGGGAACCCAAAGAACAATTACTGCTTCCAGAAGCAGTTAGTGGATGGCCCACCTGAGAAAATCGGTGGAGTTCAGTTGCAGCTGAAGAGCAAGTTCCCTTTTGATGTAATGTGGAGTGAAGACCCACATTTTCATGCAAAGTGGAAGTCGGATCATGAGATTGGGCACTTTGAGTTTGAAGGTGAGGCATCATGCAGCTCGTATTCGGATGGGAAGTTGCTAGATGTGGCTGGGAGGGGTTGTTCTGTTTCGGGGAATGAAATGAGTTTTGAGTCTGGTGCGGTGAATTTGGAAAAATGCTTTGATCATAAGGGAAAGAAGGTCGGTAGTCGAAGGCCAAGTCATATTCTGCGCCGCAGGCGCAAGGGGGCCTCTGTGAAATGTGGAAAGCTGTTTTCAGTTTCCTCAAGTGATATGGTACAGTCAGATTCCCAAGACATGGCTGGACCTCCTTACAGAATTAGTAAAATGGGGGACGAGGTTACTGAAGTTCCCATCAATTCAGGAAGGCCAAGTCATGGTATTCACCGCCGCCGCAAGGGGCGTTCTGTTGATTATGGAAAGATATTTTCGGTTTCACCATATGATATTTCAGATAGAAGAGACATTCCTACACCTCCTTCCAGCATAAGTAAAGTGGGGAATGACGGTACTGAAGTTCCTTCCACTTCAGATGGCATGGAAGCTCCAACAGACACTGGTGGGCCAGAAAGAGTTTCGCATCTTGAGTTGAATGAACATGTTGGTAAAACTAAATCAATTCAGAATGATGCTTCTTCTGTTGAAAATTTAGGCTGCAATGAGCAAGAGAAGCCAGTTTATGACGGTAATGATAAAACTATGGTTCGGGTATTGGAACAAGCACTAGATGAAGAGCATACTGCTCGTACTGCTCTCTATTATGAATTGGAGAAGGAGAGAAGTGCTGCTGCTACTGCGGCAGATGAGGCCATGGCAATGATATTGCGTCTGCAAGAAGAGAAGGCATCAATTGAAATGGAGGCAAGGCAATACCAGAGGATGATACGGGAAAAATCAGCTTATGATGCTGAGGAAATGAACATTCTCAAAGAGATCTTACTTAGGAGAGAAAGGGAAAAGCATTTCTTGGAGAAGGAAGTTGAATACTACAGGCAAATACTTTTTGGAAACTATCAAGTTGATGATGACATGCATGATGTTGCTGCCACACAAGCACAAAGTATTTCTCTGCATTCAAGTGAAGAGCTGGTGTCAATGCAGGAGCGGACAAGCTATTCAATTAGTGAGAAGTCAAAGTTGAAAATGGCAAATAGTTCTCCAGATTACAGGGCCCTATCAAGTGACTCACAAAATCGCAGTCTGGCCTTGGGGAAAGAACTACCAGTTCTGGATTTGGAAGAAGATGACACTTCGAAGCATGTGGACATGCACTTGCTTCCAAGCACTGACAGCCATTCACATATCTTAAATAGTAGGTATGAGATCAGTCACGAGTTGCAAGAGAAGGGAATGGTGTCTGTGGATGAGAGACCAGTTTCTCGGGGAAAAGAGGTCCAGGTAGTGAATGCTGAGGGACATGAATTGGTTGAAAAAACTATTCCTCCGAACGAACAGGAACTTGCTTTAGCTGGTAGCAATGTGGATCATGGAAGCAAAGATCCACCTAGCTTAACGTCTAATACAGAGCCTCGTGTTCATGATATTCATGTTATTGACGATAAGTCTGATTTGTATAATGAAAAGAGTGCAGAGAAAAGTGAACAGCTAGTAGCAAATGCTACCTTAGATATTTCTGAAAAACACAAGAGTATGACAGGGTTGGGAACTGAGCTTAAAGTGCACAAAGTTGGTTCAGACAGTGGATTGCCAATAGTGGATGGCTCTCAAGGCAATGCTTTACCTTACAATATGCGGAGGAATTCCATGTCTGAGGTTGATTATGAAAGATCAAAAATAGACAGTAAACTGCACCGAGGTGGTCCAGACATGCCAACTAATTGCATGCGGAGGAATTCCATGTCCGCAATTGATTATGACAGATGGAAGATTGACAACAAAGTGGACAGAGGTAGCTCAGACACGGAACCTAAAGATGTGCGGAGGAATTCCATGACTGCAGTTGATTATGAAAGATGGAAAATGGCCAATAAATCATACAGAGGTAGTTCAAACACGTCGCCATCACCTTCTGTCATGCGAAGAAATTCCATGTCTGCAGTTGATTATGAAAGGTGCAAACTTGACAATGAAGTTGAGTGGCTTAGAGAAAGGTTAAGGATTGTGCAAGAGGGAAGAGAGAAGCTCAACCTCTTAGTGGGCCACAGAGAAAGGGAAAAAGTTCAGCTGAAACTTTTGGAAGATATAGCAGGCCAACTCCAAGAGATTCGGCAATTGACGGAACCTGGAAAGGTCAAGTGCCAGGCTGCATTGCCGCCTCCATCATCTAAGGTTATGTCAAAGAAAAGACGATGGCGAACTTTGTCTCTAGGAGTGCATAGAAATCGGCGATGTGCTGGATCAGTTGCCGTCATCGTCGACCCCGAATGCATCTCCGATTTCAAGACAACTGCCACACGAAGAAAACGCAAGAAGAAGAAGATCGTTGCAATTGCAGCTTCTGCAAGTCCTGGAGTTCGGAATCGGGTTTTGTTCCAGGTCGAGGCTCGCACCGGTGTCGTTTGTCCAAAGCTCACGGAGGCCACTCTAGTCTCGATTCAGCCAGTCCTTCAACCTCGTCGCCCAAAAGTCGTTTTTTCCGACCTCGCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

974

Amino Acids

109.03

Weight (kDa)

6.88

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 341 - 430 2.1e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 607, 686
AccBSI CCGCTC 1 cut(s) 1414
AccI GTMKAC 1 cut(s) 2685
AclWI GGATC 4 cut(s) 327, 1800, 1802, 2674
AcoI YGGCCR 1 cut(s) 2292
AcsI RAATTY 7 cut(s) 124, 454, 955, 2068, 2161, 2254, 2353
AcuI CTGAAG 4 cut(s) 272, 675, 822, 843
AfaI GTAC 3 cut(s) 588, 820, 1057
AfiI CCNNNNNNNGG 6 cut(s) 158, 607, 686, 706, 2464, 2809
AflIII ACRYGT 2 cut(s) 907, 2322
AgeI ACCGGT 1 cut(s) 2819
AhdI GACNNNNNGTC 1 cut(s) 1870
AjiI CACGTC 1 cut(s) 2325
AjnI CCWGG 5 cut(s) 1704, 2551, 2568, 2776, 2802
AleI CACNNNNGTG 1 cut(s) 2124
AloI GAACNNNNNNTCC 2 cut(s) 394, 426
Alw21I GWGCWC 1 cut(s) 1992
Alw26I GTCTC 4 cut(s) 765, 1672, 2637, 2863
Alw44I GTGCAC 1 cut(s) 1988
AlwI GGATC 4 cut(s) 327, 1800, 1802, 2674
AlwNI CAGNNNCTG 2 cut(s) 866, 2768
Ama87I CYCGRG 1 cut(s) 1689
ApaI GGGCCC 1 cut(s) 1483
ApaLI GTGCAC 1 cut(s) 1988
ApeKI GCWGC 8 cut(s) 247, 363, 963, 1094, 1097, 1352, 2573, 2762
ApoI RAATTY 7 cut(s) 124, 454, 955, 2068, 2161, 2254, 2353
ArsI GACNNNNNNTTYG 6 cut(s) 299, 331, 493, 525, 1492, 1524
AsiGI ACCGGT 1 cut(s) 2819
Asp700I GAANNNNTTC 2 cut(s) 2353, 2487
AspLEI GCGC 2 cut(s) 522, 531
AspS9I GGNCC 9 cut(s) 215, 537, 614, 872, 1479, 1480, 1702, 2129, 2467
AsuHPI GGTGA 5 cut(s) 362, 463, 686, 738, 2325
AsuII TTCGAA 1 cut(s) 1562
AvaI CYCGRG 1 cut(s) 1689
AvaII GGWCC 3 cut(s) 614, 1702, 2129
BaeGI GKGCMC 3 cut(s) 336, 1483, 1992
BaeI ACNNNNGTAYC 2 cut(s) 810, 843
BalI TGGCCA 1 cut(s) 2294
BanII GRGCYC 1 cut(s) 1483
BauI CACGAG 2 cut(s) 1640, 1839
BbsI GAAGAC 2 cut(s) 134, 294
Bbv12I GWGCWC 1 cut(s) 1992
BbvCI CCTCAGC 2 cut(s) 1218, 1719
BbvI GCAGC 8 cut(s) 259, 375, 950, 1081, 1084, 1339, 2560, 2774
BceAI ACGGC 1 cut(s) 2660
BcgI CGANNNNNNTGC 6 cut(s) 1034, 1068, 2633, 2667, 2806, 2840
BciT130I CCWGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 4 cut(s) 765, 1672, 2637, 2863
BfaI CTAG 6 cut(s) 389, 1034, 1815, 1916, 2636, 2855
BfmI CTRYAG 3 cut(s) 1297, 2265, 2364
BglII AGATCT 1 cut(s) 1242
Bme1390I CCNGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
Bme18I GGWCC 3 cut(s) 614, 1702, 2129
BmeRI GACNNNNNGTC 1 cut(s) 1870
BmeT110I CYCGRG 1 cut(s) 1689
BmgBI CACGTC 1 cut(s) 2325
BmgT120I GGNCC 9 cut(s) 215, 537, 614, 872, 1479, 1480, 1702, 2129, 2467
BmiI GGNNCC 5 cut(s) 173, 538, 1481, 2235, 2550
BmrFI CCNGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
BmsI GCATC 6 cut(s) 365, 898, 928, 1163, 1204, 2706
BpiI GAAGAC 2 cut(s) 134, 294
BplI GAGNNNNNCTC 2 cut(s) 2436, 2468
BpmI CTGGAG 2 cut(s) 1452, 2799
Bpu10I CCTNAGC 2 cut(s) 1218, 1719
Bpu14I TTCGAA 1 cut(s) 1562
BpuEI CTTGAG 3 cut(s) 559, 914, 2019
BsaI GGTCTC 1 cut(s) 1672
BsaJI CCNNGG 3 cut(s) 1119, 1515, 2122
BsaWI WCCGGW 1 cut(s) 2819
BsaXI ACNNNNNCTCC 4 cut(s) 394, 424, 1735, 1765
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 607, 686, 706, 2464, 2809
Bse118I RCCGGY 1 cut(s) 2819
Bse1I ACTGG 5 cut(s) 871, 983, 1532, 1682, 2870
Bse3DI GCAATG 5 cut(s) 973, 1131, 1792, 2047, 2576
BseBI CCWGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
BseDI CCNNGG 3 cut(s) 1119, 1515, 2122
BseGI GGATG 5 cut(s) 217, 382, 1194, 1678, 2032
BseLI CCNNNNNNNGG 6 cut(s) 158, 607, 686, 706, 2464, 2809
BseMI GCAATG 5 cut(s) 973, 1131, 1792, 2047, 2576
BseMII CTCAG 6 cut(s) 213, 1209, 1710, 1968, 2070, 2238
BseNI ACTGG 5 cut(s) 871, 983, 1532, 1682, 2870
BseSI GKGCMC 3 cut(s) 336, 1483, 1992
BseXI GCAGC 8 cut(s) 259, 375, 950, 1081, 1084, 1339, 2560, 2774
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 2 cut(s) 1917, 2102
BshTI ACCGGT 1 cut(s) 2819
BsiHKAI GWGCWC 1 cut(s) 1992
BsiHKCI CYCGRG 1 cut(s) 1689
BsiSI CCGG 1 cut(s) 2820
BslFI GGGAC 2 cut(s) 656, 1737
BslI CCNNNNNNNGG 6 cut(s) 158, 607, 686, 706, 2464, 2809
BsmAI GTCTC 4 cut(s) 765, 1672, 2637, 2863
BsmFI GGGAC 2 cut(s) 656, 1737
BsmI GAATGC 3 cut(s) 1381, 1720, 2699
Bso31I GGTCTC 1 cut(s) 1672
BsoBI CYCGRG 1 cut(s) 1689
Bsp119I TTCGAA 1 cut(s) 1562
Bsp120I GGGCCC 1 cut(s) 1479
Bsp1286I GDGCHC 3 cut(s) 336, 1483, 1992
Bsp19I CCATGG 1 cut(s) 1119
BspCNI CTCAG 6 cut(s) 214, 1210, 1711, 1969, 2071, 2237
BspHI TCATGA 2 cut(s) 322, 1846
BspLI GGNNCC 5 cut(s) 173, 538, 1481, 2235, 2550
BspMAI CTGCAG 2 cut(s) 2269, 2368
BspPI GGATC 4 cut(s) 327, 1800, 1802, 2674
BspQI GCTCTTC 3 cut(s) 248, 1035, 1386
BspT104I TTCGAA 1 cut(s) 1562
BspTNI GGTCTC 1 cut(s) 1672
BsrBI CCGCTC 1 cut(s) 1414
BsrDI GCAATG 5 cut(s) 973, 1131, 1792, 2047, 2576
BsrFI RCCGGY 1 cut(s) 2819
BsrI ACTGG 5 cut(s) 871, 983, 1532, 1682, 2870
BssAI RCCGGY 1 cut(s) 2819
BssECI CCNNGG 3 cut(s) 1119, 1515, 2122
BssSI CACGAG 2 cut(s) 1640, 1839
BssT1I CCWWGG 2 cut(s) 1119, 1515
Bst2BI CACGAG 2 cut(s) 1640, 1839
Bst2UI CCWGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
Bst4CI ACNGT 5 cut(s) 591, 818, 995, 2009, 2111
Bst6I CTCTTC 6 cut(s) 248, 763, 1035, 1140, 1386, 2437
BstAPI GCANNNNNTGC 1 cut(s) 2768
BstBI TTCGAA 1 cut(s) 1562
BstC8I GCNNGC 5 cut(s) 7, 527, 2153, 2518, 2815
BstDSI CCRYGG 1 cut(s) 1119
BstF5I GGATG 5 cut(s) 217, 382, 1194, 1678, 2032
BstHHI GCGC 2 cut(s) 522, 531
BstMAI GTCTC 4 cut(s) 765, 1672, 2637, 2863
BstNI CCWGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
BstNSI RCATGY 7 cut(s) 9, 911, 1342, 1571, 1579, 2140, 2155
BstSCI CCNGG 5 cut(s) 1704, 2551, 2568, 2776, 2802
BstSFI CTRYAG 3 cut(s) 1297, 2265, 2364
BstSLI GKGCMC 3 cut(s) 336, 1483, 1992
BstV1I GCAGC 8 cut(s) 259, 375, 950, 1081, 1084, 1339, 2560, 2774
BstV2I GAAGAC 2 cut(s) 134, 294
BstX2I RGATCY 2 cut(s) 1242, 1807
BstYI RGATCY 2 cut(s) 1242, 1807
BtgI CCRYGG 1 cut(s) 1119
BtgZI GCGATG 1 cut(s) 2671
BtrI CACGTC 1 cut(s) 2325
BtsCI GGATG 5 cut(s) 217, 382, 1194, 1678, 2032
BtsIMutI CAGTG 4 cut(s) 40, 864, 1593, 2014
Cac8I GCNNGC 5 cut(s) 7, 527, 2153, 2518, 2815
CaiI CAGNNNCTG 2 cut(s) 866, 2768
CciI TCATGA 2 cut(s) 322, 1846
CfoI GCGC 2 cut(s) 522, 531
Cfr10I RCCGGY 1 cut(s) 2819
Cfr13I GGNCC 9 cut(s) 215, 537, 614, 872, 1479, 1480, 1702, 2129, 2467
CseI GACGC 2 cut(s) 78, 1124
Csp6I GTAC 3 cut(s) 587, 819, 1056
CspAI ACCGGT 1 cut(s) 2819
CviQI GTAC 3 cut(s) 587, 819, 1056
DriI GACNNNNNGTC 1 cut(s) 1870
EaeI YGGCCR 1 cut(s) 2292
Eam1104I CTCTTC 6 cut(s) 248, 763, 1035, 1140, 1386, 2437
Eam1105I GACNNNNNGTC 1 cut(s) 1870
EarI CTCTTC 6 cut(s) 248, 763, 1035, 1140, 1386, 2437
Eco130I CCWWGG 2 cut(s) 1119, 1515
Eco24I GRGCYC 1 cut(s) 1483
Eco31I GGTCTC 1 cut(s) 1672
Eco47I GGWCC 3 cut(s) 614, 1702, 2129
Eco57I CTGAAG 4 cut(s) 272, 675, 822, 843
Eco88I CYCGRG 1 cut(s) 1689
EcoO109I RGGNCCY 3 cut(s) 537, 1479, 1480
EcoRI GAATTC 3 cut(s) 2068, 2161, 2254
EcoRII CCWGG 5 cut(s) 1704, 2551, 2568, 2776, 2802
EcoT14I CCWWGG 2 cut(s) 1119, 1515
EcoT22I ATGCAT 2 cut(s) 1344, 2699
EcoT38I GRGCYC 1 cut(s) 1483
ErhI CCWWGG 2 cut(s) 1119, 1515
FalI AAGNNNNNCTT 2 cut(s) 1259, 1291
FaqI GGGAC 2 cut(s) 656, 1737
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 1 cut(s) 472
FblI GTMKAC 1 cut(s) 2685
FokI GGATG 5 cut(s) 224, 389, 1201, 1685, 2039
FriOI GRGCYC 1 cut(s) 1483
FspBI CTAG 6 cut(s) 389, 1034, 1815, 1916, 2636, 2855
GlaI GCGC 2 cut(s) 521, 530
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 2 cut(s) 1452, 2799
HapII CCGG 1 cut(s) 2820
HgaI GACGC 2 cut(s) 78, 1124
HhaI GCGC 2 cut(s) 522, 531
Hin6I GCGC 2 cut(s) 520, 529
HinP1I GCGC 2 cut(s) 520, 529
HincII GTYRAC 1 cut(s) 2686
HindII GTYRAC 1 cut(s) 2686
HinfI GANTC 6 cut(s) 440, 596, 1493, 2533, 2788, 2863
HpaII CCGG 1 cut(s) 2820
HphI GGTGA 5 cut(s) 362, 463, 686, 738, 2325
Hpy166II GTNNAC 6 cut(s) 1573, 1910, 1990, 2114, 2212, 2686
Hpy8I GTNNAC 6 cut(s) 1573, 1910, 1990, 2114, 2212, 2686
Hpy99I CGWCG 3 cut(s) 2329, 2687, 2889
HpyCH4III ACNGT 5 cut(s) 591, 818, 995, 2009, 2111
HpyCH4IV ACGT 2 cut(s) 1823, 2324
HpySE526I ACGT 2 cut(s) 1823, 2324
HspAI GCGC 2 cut(s) 520, 529
Ksp22I TGATCA 1 cut(s) 472
LguI GCTCTTC 3 cut(s) 248, 1035, 1386
LmnI GCTCC 2 cut(s) 859, 1411
Lsp1109I GCAGC 8 cut(s) 259, 375, 950, 1081, 1084, 1339, 2560, 2774
LweI GCATC 6 cut(s) 365, 898, 928, 1163, 1204, 2706
MaeI CTAG 6 cut(s) 389, 1034, 1815, 1916, 2636, 2855
MaeII ACGT 2 cut(s) 1823, 2324
MaeIII GTNAC 3 cut(s) 649, 1490, 1637
MbiI CCGCTC 1 cut(s) 1414
MfeI CAATTG 4 cut(s) 1158, 2175, 2540, 2757
MflI RGATCY 2 cut(s) 1242, 1807
MhlI GDGCHC 3 cut(s) 336, 1483, 1992
MlsI TGGCCA 1 cut(s) 2294
MluNI TGGCCA 1 cut(s) 2294
MlyI GAGTC 2 cut(s) 449, 1487
MmeI TCCRAC 2 cut(s) 297, 881
Mox20I TGGCCA 1 cut(s) 2294
Mph1103I ATGCAT 2 cut(s) 1344, 2699
MroXI GAANNNNTTC 2 cut(s) 2353, 2487
MscI TGGCCA 1 cut(s) 2294
MseI TTAA 5 cut(s) 137, 1616, 1820, 1983, 2423
MslI CAYNNNNRTG 4 cut(s) 300, 1124, 1386, 2124
Msp20I TGGCCA 1 cut(s) 2294
MspA1I CMGCKG 2 cut(s) 250, 2494
MspI CCGG 1 cut(s) 2820
MspR9I CCNGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
MunI CAATTG 4 cut(s) 1158, 2175, 2540, 2757
Mva1269I GAATGC 3 cut(s) 1381, 1720, 2699
MvaI CCWGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
NcoI CCATGG 1 cut(s) 1119
NdeI CATATG 1 cut(s) 751
NlaIV GGNNCC 5 cut(s) 173, 538, 1481, 2235, 2550
NmuCI GTSAC 2 cut(s) 1490, 1637
NsiI ATGCAT 2 cut(s) 1344, 2699
NspI RCATGY 7 cut(s) 9, 911, 1342, 1571, 1579, 2140, 2155
NspV TTCGAA 1 cut(s) 1562
OliI CACNNNNGTG 1 cut(s) 2124
PaeI GCATGC 2 cut(s) 9, 2155
PagI TCATGA 2 cut(s) 322, 1846
PciI ACATGT 1 cut(s) 907
PciSI GCTCTTC 3 cut(s) 248, 1035, 1386
PcsI WCGNNNNNNNCGW 2 cut(s) 2688, 2904
PctI GAATGC 3 cut(s) 1381, 1720, 2699
PdmI GAANNNNTTC 2 cut(s) 2353, 2487
PfeI GAWTC 4 cut(s) 596, 2533, 2788, 2863
PflMI CCANNNNNTGG 2 cut(s) 607, 686
PfoI TCCNGGA 1 cut(s) 2776
PinAI ACCGGT 1 cut(s) 2819
PleI GAGTC 2 cut(s) 448, 1487
PpsI GAGTC 2 cut(s) 448, 1487
PscI ACATGT 1 cut(s) 907
Psp6I CCWGG 5 cut(s) 1704, 2551, 2568, 2776, 2802
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 5 cut(s) 1704, 2551, 2568, 2776, 2802
PspN4I GGNNCC 5 cut(s) 173, 538, 1481, 2235, 2550
PspOMI GGGCCC 1 cut(s) 1479
PspPI GGNCC 9 cut(s) 215, 537, 614, 872, 1479, 1480, 1702, 2129, 2467
PstI CTGCAG 2 cut(s) 2269, 2368
PstNI CAGNNNCTG 2 cut(s) 866, 2768
PsuI RGATCY 2 cut(s) 1242, 1807
PvuII CAGCTG 2 cut(s) 250, 2494
RsaI GTAC 3 cut(s) 588, 820, 1057
RsaNI GTAC 3 cut(s) 587, 819, 1056
RseI CAYNNNNRTG 4 cut(s) 300, 1124, 1386, 2124
SalI GTCGAC 1 cut(s) 2684
SapI GCTCTTC 3 cut(s) 248, 1035, 1386
SaqAI TTAA 5 cut(s) 137, 1616, 1820, 1983, 2423
Sau96I GGNCC 9 cut(s) 215, 537, 614, 872, 1479, 1480, 1702, 2129, 2467
SchI GAGTC 2 cut(s) 449, 1487
ScrFI CCNGG 5 cut(s) 1706, 2553, 2570, 2778, 2804
SduI GDGCHC 3 cut(s) 336, 1483, 1992
SfaNI GCATC 6 cut(s) 365, 898, 928, 1163, 1204, 2706
SfcI CTRYAG 3 cut(s) 1297, 2265, 2364
SfuI TTCGAA 1 cut(s) 1562
SgrAI CRCCGGYG 1 cut(s) 2819
SinI GGWCC 3 cut(s) 614, 1702, 2129
SmiMI CAYNNNNRTG 4 cut(s) 300, 1124, 1386, 2124
SmlI CTYRAG 3 cut(s) 574, 893, 2034
SmoI CTYRAG 3 cut(s) 574, 893, 2034
SphI GCATGC 2 cut(s) 9, 2155
SspMI CTAG 6 cut(s) 389, 1034, 1815, 1916, 2636, 2855
StyD4I CCNGG 5 cut(s) 1704, 2551, 2568, 2776, 2802
StyI CCWWGG 2 cut(s) 1119, 1515
TaaI ACNGT 5 cut(s) 591, 818, 995, 2009, 2111
TaiI ACGT 2 cut(s) 1826, 2327
TaqI TCGA 6 cut(s) 113, 499, 1562, 2685, 2808, 2861
TauI GCSGC 5 cut(s) 525, 702, 705, 1109, 2584
TfiI GAWTC 4 cut(s) 596, 2533, 2788, 2863
Tru1I TTAA 5 cut(s) 137, 1616, 1820, 1983, 2423
Tru9I TTAA 5 cut(s) 137, 1616, 1820, 1983, 2423
TscAI CASTG 4 cut(s) 40, 871, 1600, 2014
TseFI GTSAC 2 cut(s) 1490, 1637
TseI GCWGC 8 cut(s) 247, 363, 963, 1094, 1097, 1352, 2573, 2762
Tsp45I GTSAC 2 cut(s) 1490, 1637
TspGWI ACGGA 3 cut(s) 2246, 2561, 2858
TspRI CASTG 4 cut(s) 40, 871, 1600, 2014
Van91I CCANNNNNTGG 2 cut(s) 607, 686
VneI GTGCAC 1 cut(s) 1988
VpaK11BI GGWCC 3 cut(s) 614, 1702, 2129
XapI RAATTY 7 cut(s) 124, 454, 955, 2068, 2161, 2254, 2353
XceI RCATGY 7 cut(s) 9, 911, 1342, 1571, 1579, 2140, 2155
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmiI GTMKAC 1 cut(s) 2685
XmnI GAANNNNTTC 2 cut(s) 2353, 2487
XspI CTAG 6 cut(s) 389, 1034, 1815, 1916, 2636, 2855
Zsp2I ATGCAT 2 cut(s) 1344, 2699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.