Rroxscaffold_5G00359120

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005611
Physical Location & Seq
Reverse (-)
39164343 .. 39170591
6249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00359120.1

Sequence Viewer

Length: 3066 bp
ATGAAAATGGCATGCCAAATGATACATTCATGGACATTCAGTGGGCTTGTGGGTGCATTTCTTGATCTTGCAATAGCCTATCTCTTACTCTGTGCCTCAGCTCTTGCGTTTTTTACCTCGAAGTTTTTGGATGTCTTTGGATTAAGTCTTCCGTGCCCTTGTGATGGGCTATTTGGGAACCCAAAGAATAATTACTGCTTCCAGAAGCAGTTAGTGGATGGCCCATCTGAGAAAATCGGTGGAGTTCAGTTGCAGCTGAAGAGCAAGTTCCCTTTTGATGTAATGTGGAGTGAAGACCCACATTTTCATGCAAAGTCGAAGTTGGATCATGAGAATGGGCACTTTGAGTTTGAAGGTGAAGCATCGTGTAGCTCTTTTTCGGATGGGAAGTTGCTAGATGTGGCTGGGAGGGGTTGCTCTGTTTCGGGGAATGAAATGAGTTTTGAGTCTGGTGCGGTGAATTTGGAAAAATGCTTTGATCACAAGGGAAAGAAGGTTGGTGGTCGAAGGCCAAGTCATAGTCTTCGCCGCCGGCGCAAGGGGGCATCTGTGGATTATGGAAAGCTGTTTTCAGTTTCCTCAAGTGATATGGTACAGTCAGATTCCCAAGACATGGCTAGACCTCCTTACAGAAATGGTAAATTGGGGGACGAGGTTACTGAAGTTCTCATCAATTCAGGAAGGTCAAGTCATGGTATTCACCGCCGCCGCAAGGGGCGTTCTGTTGATTATGGAAAGATATTTTCGGTTTCACCATATGATATTTCAGATAGAAGAGACATTCCTACACCTCCTTCCAGCATAAGTAAAGTGGGGAATGACGGTAGTGAAGTTCCTTCCAGTTCTGATGGCATGGAAGCTCCAACAGACACTGGTGGGCCAGAAAGAGTTTCGTGTCTTGAGTTGAATGAACATGTTGGTACAACTAAATCAATTCAGAATGATGCTTCTTCTGTTGAAAATTTAGGCTGCAATGAGCAAGAGAAGCCAGTTTATGACAGTACTGATAAAACTATGGTTCGGGTATTGGAACAAGCACTAGATGAAGAGCATACTGCTCGTACTGCTCTCTATTATGAATTGGAGAAGGAGAGAAGTGCTGCTGCTACTGCGGCAGATGAGGCCATGGCAATGATATTGCGTCTGCAAGAAGAGAAGGCATCAATTGAAATGGAGGCAAGGCAATACCAGAGGATGATACAGGAAAAATCAGCTTATGATGCTGAGGAAATGAACATTCTCAAAGAGATCTTACTTAGGAGAGAAAGGGAAAAGCATTTCTTGGAGAAGGAAGCAAATACTTTTGGAAACGATCAAGTTGATGATGACATGCATGATGTTGCTGCCACACAAGCACAAAGTATTTCTCTGCATTCAACTGAAGAGCTGGTGTCGATGCAGGAGCGGACAAGCTATTCAATTAGTGAGAAGTCAAAGTTGAAAATGGCAAATAGTTCTCCAGATTACGGGGCCCTATCAAGTGACTCACAAAATCGCACTCTGGCCTTGGGGAAAGAACTACCAGTTCTGGATTTGGAAGAAGATGACACTTCGAAGCATGTGGACATGCACTTGCTTCCAAGCACTGACAGTCATTCACATATCTTAAATAGTAGGTATGAGATCAGTCACGAGTTTCAAGAGAAGGGAATGGTGTCTGTGGATGAGAGACCAGTTTCTCGGGGAAAAGAGGTCCAGGTAGTGAATGCTGAGGGACTTGAATTGGTTGAAAAAACTATTCCTCTGAACGAACAGGAACTTGCTTTAGCTGGTAGCAATGTGGATCATGGAAGCAAAGATCCACCTAGCTTAACGTCTAATACAGAGCCTCGTGTTCATGATATTCATGTTATTGACGATAAGTCTGATTTGTATAATGAAAAGAGTGCAGAGAAAAGTGAACAGCTAGTAGCAAATGCTACCTTAGATATTTCTGAAAAACACAAGAGTATGACAGGGTTGGGAACTGAGCTTAAAGTGCACAAAGTTGGTTCAGACAGTGGATTGCCAATAGTGGATGGCTCTCAAGGCAAAGCTTTACCTTACAATATGCGGAGGAATTCCATGTCTGAGGTTGATTATGAAAGATCGAAAATAGACAGTAAACTGCACCGAGGTGGTTCAGACACGGCACCTAATTGCATGCGGAGGAATTCCATGTCTGCAATTGATTATGACAGATGGAAAATTGACAACAAAGTGGACAGAGGTAGCTCACTAAGCTCAGACACGGAACCTAAAGATGTGCGGAGGAATTCCATGACTGCAGTTGATTATGAAAGATGGAAAATGGCCAATAAATCATACAGAGGTAGTTCAGACACATCGCCATCACCTTCTGTCATGCGAAGAAATTCCATGTCTGCAGTTGATTATGAAAGATGCAAACTTGACAATGAAGTGGAGTGGCTTAGAGAAAGGTTAAGGATTGTGCAAGAGGGAAGAGAGAAGCTCAACCTCTCAGTGGGCCACAGAGAAAGGGAAAAAGTTCAGCTGAAACTTTTGGAAGATATAGCAGGCCAACTCCAAGAGATTCGGCAATTGACGGAACCTGGAAAGGTTAAGTGCCAGGCTGCATTGCCGCCTCCATCATCTAAGAAGCATAGCATGTGGGATGGAAATTCTGTCATCAAACTGGACGAAAGCTGCCTATTAAATCAGATGCAGGAGATGGCTGGATTATCATCAGTAGTGAATAAGAGCTCCATCATAAGAGCAAATTCCTCTCAGACTCCGAGTTGGCGGCCGATTCGCAAGCCGGTCCCGAACCGTGCAACAAGGCATCCGGGGGTATGTAGCCGTCGCCGTCCCGTCCACCTTGCTCAACATCATTGCCACCTCTTCTGGCTCGCGGCTCTTCTCCCGGCCGCCGTAGAGAAATCCGACCGGTCCCTAGGATATTTCCGTGCGAACACTCGTAGGAGTCCCCATGCCCGCCGGCCGGGCTTGTGGTCGGATGGTAGGGGATCCGAGGAGGAAGACGACGCCGTTCCGGAGCTAAAGGACGGTGGGTCGGAGCGGGAAAAGGCGGAGCGGTCCTTCTTAGAGTGGAGGAGGCGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1021

Amino Acids

114.3

Weight (kDa)

6.08

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 343 - 431 4.5e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2131
AccB7I CCANNNNNTGG 1 cut(s) 613
AccBSI CCGCTC 3 cut(s) 1405, 3019, 3034
AccII CGCG 1 cut(s) 2852
AccIII TCCGGA 1 cut(s) 2992
AclWI GGATC 5 cut(s) 333, 1791, 1793, 2961, 2974
AcoI YGGCCR 4 cut(s) 2292, 2744, 2865, 2939
AcsI RAATTY 8 cut(s) 460, 961, 2059, 2152, 2254, 2353, 2620, 2719
AcuI CTGAAG 3 cut(s) 278, 681, 1401
AcyI GRCGYC 1 cut(s) 2985
AfaI GTAC 4 cut(s) 594, 922, 1003, 1063
AfiI CCNNNNNNNGG 7 cut(s) 164, 538, 613, 712, 1466, 2464, 2941
AflIII ACRYGT 1 cut(s) 913
AgeI ACCGGT 1 cut(s) 2886
AhdI GACNNNNNGTC 2 cut(s) 1861, 3010
AjnI CCWGG 3 cut(s) 1695, 2551, 2568
AleI CACNNNNGTG 1 cut(s) 2115
Alw21I GWGCWC 2 cut(s) 1983, 2705
Alw26I GTCTC 2 cut(s) 771, 1663
Alw44I GTGCAC 1 cut(s) 1979
AlwI GGATC 5 cut(s) 333, 1791, 1793, 2961, 2974
AlwNI CAGNNNCTG 1 cut(s) 872
Ama87I CYCGRG 1 cut(s) 1680
Aor13HI TCCGGA 1 cut(s) 2992
ApaI GGGCCC 1 cut(s) 1474
ApaLI GTGCAC 1 cut(s) 1979
ApeKI GCWGC 7 cut(s) 253, 969, 1100, 1103, 1343, 2573, 2646
ApoI RAATTY 8 cut(s) 460, 961, 2059, 2152, 2254, 2353, 2620, 2719
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
AsiGI ACCGGT 1 cut(s) 2886
Asp700I GAANNNNTTC 2 cut(s) 2353, 2487
AspA2I CCTAGG 1 cut(s) 2893
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 9 cut(s) 221, 878, 1470, 1471, 1693, 2467, 2761, 2889, 3036
AsuC2I CCSGG 3 cut(s) 2787, 2864, 2943
AsuHPI GGTGA 5 cut(s) 368, 469, 692, 744, 2325
AsuII TTCGAA 1 cut(s) 1553
AvaI CYCGRG 1 cut(s) 1680
AvaII GGWCC 4 cut(s) 1693, 2761, 2889, 3036
AvrII CCTAGG 1 cut(s) 2893
BaeGI GKGCMC 4 cut(s) 158, 342, 1474, 1983
BalI TGGCCA 1 cut(s) 2294
BamHI GGATCC 1 cut(s) 2966
BanI GGYRCC 1 cut(s) 2131
BanII GRGCYC 2 cut(s) 1474, 2705
BauI CACGAG 2 cut(s) 1631, 1830
BbsI GAAGAC 4 cut(s) 140, 300, 515, 2985
Bbv12I GWGCWC 2 cut(s) 1983, 2705
BbvCI CCTCAGC 3 cut(s) 97, 1224, 1710
BbvI GCAGC 7 cut(s) 265, 956, 1087, 1090, 1330, 2560, 2633
BceAI ACGGC 5 cut(s) 2145, 2784, 2790, 2855, 2972
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BciT130I CCWGG 3 cut(s) 1697, 2553, 2570
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 3 cut(s) 2787, 2864, 2943
BcoDI GTCTC 2 cut(s) 771, 1663
BfaI CTAG 6 cut(s) 395, 618, 1040, 1806, 1907, 2894
BfmI CTRYAG 2 cut(s) 2265, 2364
BglI GCCNNNNNGGC 1 cut(s) 2943
BglII AGATCT 1 cut(s) 1248
BlnI CCTAGG 1 cut(s) 2893
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 6 cut(s) 1697, 2553, 2570, 2787, 2864, 2943
Bme18I GGWCC 4 cut(s) 1693, 2761, 2889, 3036
BmeRI GACNNNNNGTC 2 cut(s) 1861, 3010
BmeT110I CYCGRG 1 cut(s) 1680
BmgT120I GGNCC 9 cut(s) 221, 878, 1470, 1471, 1693, 2467, 2761, 2889, 3036
BmiI GGNNCC 9 cut(s) 179, 1471, 1472, 2133, 2235, 2550, 2763, 2891, 2968
BmrFI CCNGG 6 cut(s) 1697, 2553, 2570, 2787, 2864, 2943
BmsI GCATC 9 cut(s) 371, 554, 934, 1169, 1210, 1386, 2372, 2652, 2791
BpiI GAAGAC 4 cut(s) 140, 300, 515, 2985
BplI GAGNNNNNCTC 2 cut(s) 2436, 2468
BpmI CTGGAG 1 cut(s) 1443
Bpu10I CCTNAGC 3 cut(s) 97, 1224, 1710
Bpu14I TTCGAA 1 cut(s) 1553
BpuEI CTTGAG 3 cut(s) 565, 920, 2010
BpuMI CCSGG 3 cut(s) 2787, 2864, 2943
BsaBI GATNNNNATC 1 cut(s) 2683
BsaHI GRCGYC 1 cut(s) 2985
BsaI GGTCTC 1 cut(s) 1663
BsaJI CCNNGG 6 cut(s) 1125, 1506, 2113, 2786, 2893, 2970
BsaWI WCCGGW 2 cut(s) 2886, 2992
BsaXI ACNNNNNCTCC 1 cut(s) 3043
Bsc4I CCNNNNNNNGG 7 cut(s) 164, 538, 613, 712, 1466, 2464, 2941
Bse118I RCCGGY 4 cut(s) 531, 2758, 2886, 2937
Bse1I ACTGG 6 cut(s) 840, 877, 989, 1523, 1673, 2640
Bse3DI GCAATG 5 cut(s) 979, 1137, 1783, 2576, 2830
Bse8I GATNNNNATC 1 cut(s) 2683
BseAI TCCGGA 1 cut(s) 2992
BseBI CCWGG 3 cut(s) 1697, 2553, 2570
BseDI CCNNGG 6 cut(s) 1125, 1506, 2113, 2786, 2893, 2970
BseGI GGATG 9 cut(s) 136, 223, 388, 1200, 1669, 2023, 2620, 2782, 2962
BseJI GATNNNNATC 1 cut(s) 2683
BseLI CCNNNNNNNGG 7 cut(s) 164, 538, 613, 712, 1466, 2464, 2941
BseMI GCAATG 5 cut(s) 979, 1137, 1783, 2576, 2830
BseMII CTCAG 9 cut(s) 111, 219, 1215, 1701, 1959, 2061, 2238, 2475, 2741
BseNI ACTGG 6 cut(s) 840, 877, 989, 1523, 1673, 2640
BseRI GAGGAG 1 cut(s) 2987
BseSI GKGCMC 4 cut(s) 158, 342, 1474, 1983
BseX3I CGGCCG 3 cut(s) 2744, 2865, 2939
BseXI GCAGC 7 cut(s) 265, 956, 1087, 1090, 1330, 2560, 2633
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 2 cut(s) 1908, 2093
Bsh1236I CGCG 1 cut(s) 2852
Bsh1285I CGRYCG 4 cut(s) 2747, 2868, 2887, 2942
BshNI GGYRCC 1 cut(s) 2131
BshTI ACCGGT 1 cut(s) 2886
BsiEI CGRYCG 4 cut(s) 2747, 2868, 2887, 2942
BsiHKAI GWGCWC 2 cut(s) 1983, 2705
BsiHKCI CYCGRG 1 cut(s) 1680
BsiSI CCGG 8 cut(s) 532, 2759, 2786, 2864, 2887, 2938, 2942, 2993
BslFI GGGAC 6 cut(s) 662, 1728, 2747, 2793, 2875, 2910
BslI CCNNNNNNNGG 7 cut(s) 164, 538, 613, 712, 1466, 2464, 2941
BsmAI GTCTC 2 cut(s) 771, 1663
BsmFI GGGAC 6 cut(s) 662, 1728, 2747, 2793, 2875, 2910
BsmI GAATGC 2 cut(s) 1372, 1711
Bso31I GGTCTC 1 cut(s) 1663
BsoBI CYCGRG 1 cut(s) 1680
Bsp119I TTCGAA 1 cut(s) 1553
Bsp120I GGGCCC 1 cut(s) 1470
Bsp1286I GDGCHC 5 cut(s) 158, 342, 1474, 1983, 2705
Bsp13I TCCGGA 1 cut(s) 2992
Bsp19I CCATGG 1 cut(s) 1125
BspCNI CTCAG 9 cut(s) 110, 220, 1216, 1702, 1960, 2062, 2237, 2474, 2740
BspEI TCCGGA 1 cut(s) 2992
BspFNI CGCG 1 cut(s) 2852
BspHI TCATGA 2 cut(s) 328, 1837
BspLI GGNNCC 9 cut(s) 179, 1471, 1472, 2133, 2235, 2550, 2763, 2891, 2968
BspMAI CTGCAG 2 cut(s) 2269, 2368
BspPI GGATC 5 cut(s) 333, 1791, 1793, 2961, 2974
BspQI GCTCTTC 4 cut(s) 254, 1041, 1377, 2862
BspT104I TTCGAA 1 cut(s) 1553
BspT107I GGYRCC 1 cut(s) 2131
BspTNI GGTCTC 1 cut(s) 1663
BsrBI CCGCTC 3 cut(s) 1405, 3019, 3034
BsrDI GCAATG 5 cut(s) 979, 1137, 1783, 2576, 2830
BsrFI RCCGGY 4 cut(s) 531, 2758, 2886, 2937
BsrI ACTGG 6 cut(s) 840, 877, 989, 1523, 1673, 2640
BssAI RCCGGY 4 cut(s) 531, 2758, 2886, 2937
BssECI CCNNGG 6 cut(s) 1125, 1506, 2113, 2786, 2893, 2970
BssNI GRCGYC 1 cut(s) 2985
BssSI CACGAG 2 cut(s) 1631, 1830
BssT1I CCWWGG 3 cut(s) 1125, 1506, 2893
Bst2BI CACGAG 2 cut(s) 1631, 1830
Bst2UI CCWGG 3 cut(s) 1697, 2553, 2570
Bst4CI ACNGT 8 cut(s) 597, 824, 1001, 1592, 2000, 2102, 2771, 3008
Bst6I CTCTTC 8 cut(s) 254, 769, 1041, 1146, 1377, 2437, 2846, 2862
BstACI GRCGYC 1 cut(s) 2985
BstBI TTCGAA 1 cut(s) 1553
BstC8I GCNNGC 8 cut(s) 13, 533, 2144, 2518, 2756, 2850, 2935, 2939
BstDSI CCRYGG 1 cut(s) 1125
BstF5I GGATG 9 cut(s) 136, 223, 388, 1200, 1669, 2023, 2620, 2782, 2962
BstFNI CGCG 1 cut(s) 2852
BstHHI GCGC 1 cut(s) 537
BstMAI GTCTC 2 cut(s) 771, 1663
BstMCI CGRYCG 4 cut(s) 2747, 2868, 2887, 2942
BstNI CCWGG 3 cut(s) 1697, 2553, 2570
BstNSI RCATGY 7 cut(s) 15, 917, 1333, 1562, 1570, 2146, 2611
BstSCI CCNGG 6 cut(s) 1695, 2551, 2568, 2785, 2862, 2941
BstSFI CTRYAG 2 cut(s) 2265, 2364
BstSLI GKGCMC 4 cut(s) 158, 342, 1474, 1983
BstUI CGCG 1 cut(s) 2852
BstV1I GCAGC 7 cut(s) 265, 956, 1087, 1090, 1330, 2560, 2633
BstV2I GAAGAC 4 cut(s) 140, 300, 515, 2985
BstX2I RGATCY 3 cut(s) 1248, 1798, 2966
BstYI RGATCY 3 cut(s) 1248, 1798, 2966
BstZI CGGCCG 3 cut(s) 2744, 2865, 2939
BtgI CCRYGG 1 cut(s) 1125
BtgZI GCGATG 1 cut(s) 2310
BtsCI GGATG 9 cut(s) 136, 223, 388, 1200, 1669, 2023, 2620, 2782, 2962
BtsIMutI CAGTG 5 cut(s) 46, 870, 1584, 2005, 2469
Cac8I GCNNGC 8 cut(s) 13, 533, 2144, 2518, 2756, 2850, 2935, 2939
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 2 cut(s) 328, 1837
CfoI GCGC 1 cut(s) 537
Cfr10I RCCGGY 4 cut(s) 531, 2758, 2886, 2937
Cfr13I GGNCC 9 cut(s) 221, 878, 1470, 1471, 1693, 2467, 2761, 2889, 3036
CseI GACGC 2 cut(s) 1130, 2993
Csp6I GTAC 4 cut(s) 593, 921, 1002, 1062
CspAI ACCGGT 1 cut(s) 2886
CviQI GTAC 4 cut(s) 593, 921, 1002, 1062
DriI GACNNNNNGTC 2 cut(s) 1861, 3010
EaeI YGGCCR 4 cut(s) 2292, 2744, 2865, 2939
EagI CGGCCG 3 cut(s) 2744, 2865, 2939
Eam1104I CTCTTC 8 cut(s) 254, 769, 1041, 1146, 1377, 2437, 2846, 2862
Eam1105I GACNNNNNGTC 2 cut(s) 1861, 3010
EarI CTCTTC 8 cut(s) 254, 769, 1041, 1146, 1377, 2437, 2846, 2862
EciI GGCGGA 1 cut(s) 3044
Ecl136II GAGCTC 1 cut(s) 2703
EclXI CGGCCG 3 cut(s) 2744, 2865, 2939
Eco130I CCWWGG 3 cut(s) 1125, 1506, 2893
Eco24I GRGCYC 2 cut(s) 1474, 2705
Eco31I GGTCTC 1 cut(s) 1663
Eco47I GGWCC 4 cut(s) 1693, 2761, 2889, 3036
Eco52I CGGCCG 3 cut(s) 2744, 2865, 2939
Eco53kI GAGCTC 1 cut(s) 2703
Eco57I CTGAAG 3 cut(s) 278, 681, 1401
Eco88I CYCGRG 1 cut(s) 1680
EcoICRI GAGCTC 1 cut(s) 2703
EcoO109I RGGNCCY 2 cut(s) 1470, 1471
EcoRI GAATTC 3 cut(s) 2059, 2152, 2254
EcoRII CCWGG 3 cut(s) 1695, 2551, 2568
EcoT14I CCWWGG 3 cut(s) 1125, 1506, 2893
EcoT22I ATGCAT 1 cut(s) 1335
EcoT38I GRGCYC 2 cut(s) 1474, 2705
ErhI CCWWGG 3 cut(s) 1125, 1506, 2893
FalI AAGNNNNNCTT 2 cut(s) 1265, 1297
FaqI GGGAC 6 cut(s) 662, 1728, 2747, 2793, 2875, 2910
FauI CCCGC 2 cut(s) 2942, 3012
FauNDI CATATG 1 cut(s) 757
FbaI TGATCA 1 cut(s) 478
FokI GGATG 9 cut(s) 143, 230, 395, 1207, 1676, 2030, 2627, 2769, 2969
FriOI GRGCYC 2 cut(s) 1474, 2705
FspBI CTAG 6 cut(s) 395, 618, 1040, 1806, 1907, 2894
GlaI GCGC 1 cut(s) 536
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 1443
HapII CCGG 8 cut(s) 532, 2759, 2786, 2864, 2887, 2938, 2942, 2993
HgaI GACGC 2 cut(s) 1130, 2993
HhaI GCGC 1 cut(s) 537
Hin1I GRCGYC 1 cut(s) 2985
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HindIII AAGCTT 1 cut(s) 2034
HinfI GANTC 7 cut(s) 446, 602, 1484, 2533, 2731, 2749, 2923
HpaII CCGG 8 cut(s) 532, 2759, 2786, 2864, 2887, 2938, 2942, 2993
HphI GGTGA 5 cut(s) 368, 469, 692, 744, 2325
Hpy166II GTNNAC 6 cut(s) 1564, 1901, 1981, 2105, 2203, 2815
Hpy8I GTNNAC 6 cut(s) 1564, 1901, 1981, 2105, 2203, 2815
Hpy99I CGWCG 2 cut(s) 2805, 2987
HpyCH4III ACNGT 8 cut(s) 597, 824, 1001, 1592, 2000, 2102, 2771, 3008
HpyCH4IV ACGT 1 cut(s) 1814
HpySE526I ACGT 1 cut(s) 1814
Hsp92I GRCGYC 1 cut(s) 2985
HspAI GCGC 1 cut(s) 535
Kpn2I TCCGGA 1 cut(s) 2992
KroI GCCGGC 2 cut(s) 531, 2937
KroNI GCCGGC 2 cut(s) 533, 2939
Ksp22I TGATCA 1 cut(s) 478
LguI GCTCTTC 4 cut(s) 254, 1041, 1377, 2862
LmnI GCTCC 6 cut(s) 865, 1402, 2708, 2995, 3016, 3031
Lsp1109I GCAGC 7 cut(s) 265, 956, 1087, 1090, 1330, 2560, 2633
LweI GCATC 9 cut(s) 371, 554, 934, 1169, 1210, 1386, 2372, 2652, 2791
MaeI CTAG 6 cut(s) 395, 618, 1040, 1806, 1907, 2894
MaeII ACGT 1 cut(s) 1814
MaeIII GTNAC 3 cut(s) 655, 1481, 1628
MbiI CCGCTC 3 cut(s) 1405, 3019, 3034
MfeI CAATTG 3 cut(s) 1164, 2166, 2540
MflI RGATCY 3 cut(s) 1248, 1798, 2966
MhlI GDGCHC 5 cut(s) 158, 342, 1474, 1983, 2705
MlsI TGGCCA 1 cut(s) 2294
MluNI TGGCCA 1 cut(s) 2294
MlyI GAGTC 4 cut(s) 455, 1478, 2725, 2932
MmeI TCCRAC 5 cut(s) 303, 887, 2907, 2934, 2994
Mox20I TGGCCA 1 cut(s) 2294
Mph1103I ATGCAT 1 cut(s) 1335
MreI CGCCGGCG 1 cut(s) 531
MroI TCCGGA 1 cut(s) 2992
MroNI GCCGGC 2 cut(s) 531, 2937
MroXI GAANNNNTTC 2 cut(s) 2353, 2487
MscI TGGCCA 1 cut(s) 2294
MseI TTAA 7 cut(s) 143, 1607, 1811, 1974, 2423, 2562, 2654
MslI CAYNNNNRTG 4 cut(s) 306, 333, 1130, 2115
Msp20I TGGCCA 1 cut(s) 2294
MspA1I CMGCKG 2 cut(s) 256, 2494
MspI CCGG 8 cut(s) 532, 2759, 2786, 2864, 2887, 2938, 2942, 2993
MspR9I CCNGG 6 cut(s) 1697, 2553, 2570, 2787, 2864, 2943
MunI CAATTG 3 cut(s) 1164, 2166, 2540
Mva1269I GAATGC 2 cut(s) 1372, 1711
MvaI CCWGG 3 cut(s) 1697, 2553, 2570
MvnI CGCG 1 cut(s) 2852
NaeI GCCGGC 2 cut(s) 533, 2939
NciI CCSGG 3 cut(s) 2787, 2864, 2943
NcoI CCATGG 1 cut(s) 1125
NdeI CATATG 1 cut(s) 757
NgoMIV GCCGGC 2 cut(s) 531, 2937
NlaIV GGNNCC 9 cut(s) 179, 1471, 1472, 2133, 2235, 2550, 2763, 2891, 2968
NmuCI GTSAC 2 cut(s) 1481, 1628
NsiI ATGCAT 1 cut(s) 1335
NspI RCATGY 7 cut(s) 15, 917, 1333, 1562, 1570, 2146, 2611
NspV TTCGAA 1 cut(s) 1553
OliI CACNNNNGTG 1 cut(s) 2115
PaeI GCATGC 2 cut(s) 15, 2146
PagI TCATGA 2 cut(s) 328, 1837
PciI ACATGT 1 cut(s) 913
PciSI GCTCTTC 4 cut(s) 254, 1041, 1377, 2862
PctI GAATGC 2 cut(s) 1372, 1711
PdiI GCCGGC 2 cut(s) 533, 2939
PdmI GAANNNNTTC 2 cut(s) 2353, 2487
PfeI GAWTC 3 cut(s) 602, 2533, 2749
PflMI CCANNNNNTGG 1 cut(s) 613
PinAI ACCGGT 1 cut(s) 2886
PleI GAGTC 4 cut(s) 454, 1478, 2725, 2931
PpsI GAGTC 4 cut(s) 454, 1478, 2725, 2931
PscI ACATGT 1 cut(s) 913
Psp124BI GAGCTC 1 cut(s) 2705
Psp6I CCWGG 3 cut(s) 1695, 2551, 2568
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 3 cut(s) 1695, 2551, 2568
PspN4I GGNNCC 9 cut(s) 179, 1471, 1472, 2133, 2235, 2550, 2763, 2891, 2968
PspOMI GGGCCC 1 cut(s) 1470
PspPI GGNCC 9 cut(s) 221, 878, 1470, 1471, 1693, 2467, 2761, 2889, 3036
PstI CTGCAG 2 cut(s) 2269, 2368
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 3 cut(s) 1248, 1798, 2966
PvuII CAGCTG 2 cut(s) 256, 2494
RsaI GTAC 4 cut(s) 594, 922, 1003, 1063
RsaNI GTAC 4 cut(s) 593, 921, 1002, 1062
RseI CAYNNNNRTG 4 cut(s) 306, 333, 1130, 2115
SacI GAGCTC 1 cut(s) 2705
SapI GCTCTTC 4 cut(s) 254, 1041, 1377, 2862
SaqAI TTAA 7 cut(s) 143, 1607, 1811, 1974, 2423, 2562, 2654
Sau96I GGNCC 9 cut(s) 221, 878, 1470, 1471, 1693, 2467, 2761, 2889, 3036
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 4 cut(s) 455, 1478, 2725, 2932
ScrFI CCNGG 6 cut(s) 1697, 2553, 2570, 2787, 2864, 2943
SduI GDGCHC 5 cut(s) 158, 342, 1474, 1983, 2705
SfaNI GCATC 9 cut(s) 371, 554, 934, 1169, 1210, 1386, 2372, 2652, 2791
SfcI CTRYAG 2 cut(s) 2265, 2364
SfuI TTCGAA 1 cut(s) 1553
SgrAI CRCCGGYG 1 cut(s) 531
SinI GGWCC 4 cut(s) 1693, 2761, 2889, 3036
SmiMI CAYNNNNRTG 4 cut(s) 306, 333, 1130, 2115
SmlI CTYRAG 3 cut(s) 580, 899, 2025
SmoI CTYRAG 3 cut(s) 580, 899, 2025
SphI GCATGC 2 cut(s) 15, 2146
SspMI CTAG 6 cut(s) 395, 618, 1040, 1806, 1907, 2894
SstI GAGCTC 1 cut(s) 2705
StyD4I CCNGG 6 cut(s) 1695, 2551, 2568, 2785, 2862, 2941
StyI CCWWGG 3 cut(s) 1125, 1506, 2893
TaaI ACNGT 8 cut(s) 597, 824, 1001, 1592, 2000, 2102, 2771, 3008
TaiI ACGT 1 cut(s) 1817
TaqI TCGA 6 cut(s) 119, 317, 505, 1394, 1553, 2090
TatI WGTACW 1 cut(s) 1001
TauI GCSGC 8 cut(s) 531, 708, 711, 1115, 2584, 2746, 2855, 2870
TfiI GAWTC 3 cut(s) 602, 2533, 2749
Tru1I TTAA 7 cut(s) 143, 1607, 1811, 1974, 2423, 2562, 2654
Tru9I TTAA 7 cut(s) 143, 1607, 1811, 1974, 2423, 2562, 2654
TscAI CASTG 5 cut(s) 46, 877, 1591, 2005, 2469
TseFI GTSAC 2 cut(s) 1481, 1628
TseI GCWGC 7 cut(s) 253, 969, 1100, 1103, 1343, 2573, 2646
Tsp45I GTSAC 2 cut(s) 1481, 1628
TspGWI ACGGA 4 cut(s) 141, 2246, 2561, 2894
TspRI CASTG 5 cut(s) 46, 877, 1591, 2005, 2469
Van91I CCANNNNNTGG 1 cut(s) 613
VneI GTGCAC 1 cut(s) 1979
VpaK11BI GGWCC 4 cut(s) 1693, 2761, 2889, 3036
XapI RAATTY 8 cut(s) 460, 961, 2059, 2152, 2254, 2353, 2620, 2719
XceI RCATGY 7 cut(s) 15, 917, 1333, 1562, 1570, 2146, 2611
XmaJI CCTAGG 1 cut(s) 2893
XmnI GAANNNNTTC 2 cut(s) 2353, 2487
XspI CTAG 6 cut(s) 395, 618, 1040, 1806, 1907, 2894
ZrmI AGTACT 1 cut(s) 1003
Zsp2I ATGCAT 1 cut(s) 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.