Rh4BG192500

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005611
Physical Location & Seq
Reverse (-)
33254655 .. 33258545
3891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG192500.1

Sequence Viewer

Length: 2664 bp
ATGAAAATGGCATGCCAAATGATACATTCATGGACTTTCAGTGGGCTTGTGGGTGCATTTCTTGATCTTGCAATAGCCTATCTCTTACTCTGTGCCTCAGCTCTTGCGTTTTTTACCTCGAAGTTTTTGGATGTCTTTGGATTAAGTCTTCCATGCCCTTGTGATGGGCTGTTTGGGAACCCAAAGAACAATTACTGCTTCCAGAAGCAGTTAGTGGATGGCCCATCTGAGAAAATCGGTGGAGTTCAGTTGCAGCTGAAGAGCAAGTTCCCTTTCGATGTAATGTGGAGTGAAGACCCACATTTTCATGCAAAGTCGAAGTCGGATCATGAGAATGGGCACTTTGGGTTTGAAGGTGAAGCATCGTGCAGCTCTTATTCGGATGGGAAGTTGCTAGATGTGGCTGGGAGGGGTTGCTCTGTTTCGGGGAATGAAATGAGTTTTGAGTCTGGTGCGGTGAATTTGGAAAAATGCTTTGATCATAAGGGAAAGAAGGTTGGTGGTCGAAGGCCAAGTCATAGTCTGCGCCGCCGGCGCAAGGGGGCATCTGTGGATTATGGAAAGCTGTTTTCAGTTTCCTCAAGTGATATGGTACAGTCAGATTCCCAAGACATGGCTGGACCTCCTTACAGAAATGGTAAAATGGGGGACGAGGTTACTGAAGTTCCCATCAATTCAGGAAGGCCAAGTCATGGTATTCACCGCCGCCGCAAGGGGCGTTCTGTTGATTATGGAAAGATATTTTCGGTTTCACCATATGATATTTCAGATAGAAGAGACATTCCTACACCTCCTTCCAGCATAAGTAAAGTGGGGAATGACGGTAGTGAAGTTCCTTCCAGTTCAGATGGCATGGAAGCTCCAACAGACACTGGTGGGCCAGAAAGAGTTTCACATCTTGAGTTGAATGAACGTGTTGGTAAAACTAAATCAATTCAGAATGATGCTTCTTCTGTTGAAAATTTTGGTTGCAATGAGCAAGAGAAGCCAGTTTATGATGGTAATGATAAAACTATGGTTCGGGTATTGGAACAAGCACTAGACGAAGAGCATACTGCTCGTACTGCTCTTTATTATGAATTGGAGAAGGAGAGAAGTGCTGCTGCTACTGCGGCAGATGAGGCCATGGCAATGATATTGCGTCTGCAAGAAGAGAAGGCATCAATTGAAATGGAGGCAAGGCAATACCAGAGGATGATACAGGAAAAATCAGCTTATGATGCTGAGGAAATGAACATTCTCAAAGAGATCTTACTTAGGAGAGAAAGGGAAAAGCATTTCTTGGAGAAGGAAGTTGAATACTACAGGCAAATACTTTTTGGAAACGATCAAGTTGATGATGACATGCATGATGTTGCTGCCACACAAGCACAAAGTATTTCTCTGCATTCAAGTGAAGAGCTGGTGTTGATGCAGGAGCGGACAAGCTATTCAATTAGTGAGAAGTCAAAGTTGAAAATGGCAAATAGTTCTCCAGATTACGGGGCCCTATCAAGTGACTCACAAAATCGCACTCTGGCCTTGGGGAAAGAACTACCAGTTCCGGATTTGGAAGAAGATGACACTTCGAAGCATGTGGACATGCACTTGCTTCCAAGCACTGACAGCCATTCACATATCTTAAATAGTAGGTATGAGATCAGTCACGAGTTTCAAGAGAAGGGAATGGTGTCTGTGGATGAGAGACCAGTTTCTCGGGGAAAAAAGATCCAGGTAGTGAATGCTGAGGGACTTGAATTGGTTGAAAAAACTATTCCTCCGAACGAACAGGAACTTGCTTTAGCTGGTAGCAATGTGGATCATGGAAGCAAAGATCCACCTAGCTTAACGTCTAATACAGAGCCTCGTGTTCATGATATTCATGTTATTGACGATAAGTCTGATTTGTATAATGAAAAGAGTGCAGAGAAAAGTGAACAGCTAGTAGCAAATGCTACCTTAGATATTTCTGAAAAACACAAGAGTATGACAGGGTTGGGAACTGAGCTTAAAGTGCACAAAGTTGGTTCAGACAGTGGATTGCCAATAGTGGATGGCTCTCAAGGCAAAGCTTTACCTTACAATATGCGGAGGAATTCCATGTCTGAGGTTGATTATGAAAGATCGAAAATAGACAGTAAACTGCACCGAGGTGGTCCAGACATGGCACCTAATTGCATGCGGAGGAATTCCATGTCTGCAATTGATTATGACAGATGGAAAATTGACAACAAAGTGGACAGAGGTAGCTCAGACACGGAACCTAAAGATGTGCGGAGGAATTCCATGACTGCAGTTGATTATGAAAGATGGAAAATGGCCAATAAATCATACAGAGGTAGTTCAGACACGTCGCCATCACCTTCTGTCATGCGAAGAAATTCCATGTCTGCAGTTGATTATGAAAGATGCAAACTTGACAATGAAGTTGAGTGGCTTAGAGAAAGGTTAAGGATTGTGCAAGAGGGAAGAGAGAAGCTCAACCTCTCAGTGGGCCACAGAGAAAGGGAAAAAGTTCAGCTGAAACTTTTGGAAGATATAGCAGGCCAACTCCAAGAGATTCGGCAATTGACGGAACCTGGAAAGGTCAAGTGCCAGGCTGCATTGCCGCCTCCATCATCTAAGGTTATGTCTAAGAAAAGACGATGGCGAACTTTGTCTCTAGGAGTGCATAGAAGTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

887

Amino Acids

99.28

Weight (kDa)

5.99

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 343 - 432 1.1e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2146
AccB7I CCANNNNNTGG 2 cut(s) 613, 692
AccBSI CCGCTC 1 cut(s) 1420
AccIII TCCGGA 1 cut(s) 1543
AclWI GGATC 4 cut(s) 333, 1702, 1806, 1808
AcoI YGGCCR 1 cut(s) 2298
AcsI RAATTY 6 cut(s) 460, 961, 2074, 2167, 2260, 2359
AcuI CTGAAG 2 cut(s) 278, 681
AfaI GTAC 3 cut(s) 594, 1063, 2659
AfiI CCNNNNNNNGG 7 cut(s) 164, 538, 613, 692, 712, 1481, 2470
AflIII ACRYGT 2 cut(s) 913, 2328
AhdI GACNNNNNGTC 1 cut(s) 1876
AjiI CACGTC 1 cut(s) 2331
AjnI CCWGG 3 cut(s) 1710, 2557, 2574
AleI CACNNNNGTG 1 cut(s) 2130
Alw21I GWGCWC 1 cut(s) 1998
Alw26I GTCTC 3 cut(s) 771, 1678, 2643
Alw44I GTGCAC 1 cut(s) 1994
AlwI GGATC 4 cut(s) 333, 1702, 1806, 1808
AlwNI CAGNNNCTG 1 cut(s) 872
Ama87I CYCGRG 1 cut(s) 1695
Aor13HI TCCGGA 1 cut(s) 1543
ApaI GGGCCC 1 cut(s) 1489
ApaLI GTGCAC 1 cut(s) 1994
ApeKI GCWGC 6 cut(s) 253, 369, 1100, 1103, 1358, 2579
ApoI RAATTY 6 cut(s) 460, 961, 2074, 2167, 2260, 2359
ArsI GACNNNNNNTTYG 4 cut(s) 305, 337, 499, 531
Asp700I GAANNNNTTC 2 cut(s) 2359, 2493
AspLEI GCGC 2 cut(s) 528, 537
AspS9I GGNCC 7 cut(s) 221, 620, 878, 1485, 1486, 2135, 2473
AsuHPI GGTGA 5 cut(s) 368, 469, 692, 744, 2331
AsuII TTCGAA 1 cut(s) 1568
AvaI CYCGRG 1 cut(s) 1695
AvaII GGWCC 2 cut(s) 620, 2135
BaeGI GKGCMC 3 cut(s) 342, 1489, 1998
BalI TGGCCA 1 cut(s) 2300
BanI GGYRCC 1 cut(s) 2146
BanII GRGCYC 1 cut(s) 1489
BauI CACGAG 2 cut(s) 1646, 1845
BbsI GAAGAC 2 cut(s) 140, 300
Bbv12I GWGCWC 1 cut(s) 1998
BbvCI CCTCAGC 3 cut(s) 97, 1224, 1725
BbvI GCAGC 6 cut(s) 265, 381, 1087, 1090, 1345, 2566
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BciT130I CCWGG 3 cut(s) 1712, 2559, 2576
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 3 cut(s) 771, 1678, 2643
BfaI CTAG 5 cut(s) 395, 1040, 1821, 1922, 2642
BfmI CTRYAG 3 cut(s) 1303, 2271, 2370
BglII AGATCT 1 cut(s) 1248
Bme1390I CCNGG 3 cut(s) 1712, 2559, 2576
Bme18I GGWCC 2 cut(s) 620, 2135
BmeRI GACNNNNNGTC 1 cut(s) 1876
BmeT110I CYCGRG 1 cut(s) 1695
BmgBI CACGTC 1 cut(s) 2331
BmgT120I GGNCC 7 cut(s) 221, 620, 878, 1485, 1486, 2135, 2473
BmiI GGNNCC 6 cut(s) 179, 1486, 1487, 2148, 2241, 2556
BmrFI CCNGG 3 cut(s) 1712, 2559, 2576
BmsI GCATC 7 cut(s) 371, 554, 934, 1169, 1210, 1401, 2378
BpiI GAAGAC 2 cut(s) 140, 300
BplI GAGNNNNNCTC 2 cut(s) 2442, 2474
BpmI CTGGAG 1 cut(s) 1458
Bpu10I CCTNAGC 3 cut(s) 97, 1224, 1725
Bpu14I TTCGAA 1 cut(s) 1568
BpuEI CTTGAG 3 cut(s) 565, 920, 2025
BsaI GGTCTC 1 cut(s) 1678
BsaJI CCNNGG 3 cut(s) 1125, 1521, 2128
BsaWI WCCGGW 1 cut(s) 1543
BsaXI ACNNNNNCTCC 2 cut(s) 1741, 1771
Bsc4I CCNNNNNNNGG 7 cut(s) 164, 538, 613, 692, 712, 1481, 2470
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 5 cut(s) 840, 877, 989, 1538, 1688
Bse3DI GCAATG 4 cut(s) 979, 1137, 1798, 2582
BseAI TCCGGA 1 cut(s) 1543
BseBI CCWGG 3 cut(s) 1712, 2559, 2576
BseDI CCNNGG 3 cut(s) 1125, 1521, 2128
BseGI GGATG 6 cut(s) 136, 223, 388, 1200, 1684, 2038
BseLI CCNNNNNNNGG 7 cut(s) 164, 538, 613, 692, 712, 1481, 2470
BseMI GCAATG 4 cut(s) 979, 1137, 1798, 2582
BseMII CTCAG 8 cut(s) 111, 219, 1215, 1716, 1974, 2076, 2244, 2481
BseNI ACTGG 5 cut(s) 840, 877, 989, 1538, 1688
BseSI GKGCMC 3 cut(s) 342, 1489, 1998
BseXI GCAGC 6 cut(s) 265, 381, 1087, 1090, 1345, 2566
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 3 cut(s) 388, 1923, 2108
BshNI GGYRCC 1 cut(s) 2146
BsiHKAI GWGCWC 1 cut(s) 1998
BsiHKCI CYCGRG 1 cut(s) 1695
BsiSI CCGG 2 cut(s) 532, 1544
BslFI GGGAC 2 cut(s) 662, 1743
BslI CCNNNNNNNGG 7 cut(s) 164, 538, 613, 692, 712, 1481, 2470
BsmAI GTCTC 3 cut(s) 771, 1678, 2643
BsmFI GGGAC 2 cut(s) 662, 1743
BsmI GAATGC 2 cut(s) 1387, 1726
Bso31I GGTCTC 1 cut(s) 1678
BsoBI CYCGRG 1 cut(s) 1695
Bsp119I TTCGAA 1 cut(s) 1568
Bsp120I GGGCCC 1 cut(s) 1485
Bsp1286I GDGCHC 3 cut(s) 342, 1489, 1998
Bsp13I TCCGGA 1 cut(s) 1543
Bsp19I CCATGG 1 cut(s) 1125
BspCNI CTCAG 8 cut(s) 110, 220, 1216, 1717, 1975, 2077, 2243, 2480
BspEI TCCGGA 1 cut(s) 1543
BspHI TCATGA 2 cut(s) 328, 1852
BspLI GGNNCC 6 cut(s) 179, 1486, 1487, 2148, 2241, 2556
BspMAI CTGCAG 2 cut(s) 2275, 2374
BspPI GGATC 4 cut(s) 333, 1702, 1806, 1808
BspQI GCTCTTC 3 cut(s) 254, 1041, 1392
BspT104I TTCGAA 1 cut(s) 1568
BspT107I GGYRCC 1 cut(s) 2146
BspTNI GGTCTC 1 cut(s) 1678
BsrBI CCGCTC 1 cut(s) 1420
BsrDI GCAATG 4 cut(s) 979, 1137, 1798, 2582
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 5 cut(s) 840, 877, 989, 1538, 1688
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 3 cut(s) 1125, 1521, 2128
BssSI CACGAG 2 cut(s) 1646, 1845
BssT1I CCWWGG 2 cut(s) 1125, 1521
Bst2BI CACGAG 2 cut(s) 1646, 1845
Bst2UI CCWGG 3 cut(s) 1712, 2559, 2576
Bst4CI ACNGT 4 cut(s) 597, 824, 2015, 2117
Bst6I CTCTTC 6 cut(s) 254, 769, 1041, 1146, 1392, 2443
BstBI TTCGAA 1 cut(s) 1568
BstC8I GCNNGC 4 cut(s) 13, 533, 2159, 2524
BstDSI CCRYGG 1 cut(s) 1125
BstF5I GGATG 6 cut(s) 136, 223, 388, 1200, 1684, 2038
BstHHI GCGC 2 cut(s) 528, 537
BstMAI GTCTC 3 cut(s) 771, 1678, 2643
BstNI CCWGG 3 cut(s) 1712, 2559, 2576
BstNSI RCATGY 5 cut(s) 15, 1348, 1577, 1585, 2161
BstSCI CCNGG 3 cut(s) 1710, 2557, 2574
BstSFI CTRYAG 3 cut(s) 1303, 2271, 2370
BstSLI GKGCMC 3 cut(s) 342, 1489, 1998
BstV1I GCAGC 6 cut(s) 265, 381, 1087, 1090, 1345, 2566
BstV2I GAAGAC 2 cut(s) 140, 300
BstX2I RGATCY 3 cut(s) 1248, 1707, 1813
BstYI RGATCY 3 cut(s) 1248, 1707, 1813
BtgI CCRYGG 1 cut(s) 1125
BtrI CACGTC 1 cut(s) 2331
BtsCI GGATG 6 cut(s) 136, 223, 388, 1200, 1684, 2038
BtsIMutI CAGTG 5 cut(s) 46, 870, 1599, 2020, 2475
Cac8I GCNNGC 4 cut(s) 13, 533, 2159, 2524
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 2 cut(s) 328, 1852
CfoI GCGC 2 cut(s) 528, 537
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 7 cut(s) 221, 620, 878, 1485, 1486, 2135, 2473
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 3 cut(s) 593, 1062, 2658
CviQI GTAC 3 cut(s) 593, 1062, 2658
DriI GACNNNNNGTC 1 cut(s) 1876
EaeI YGGCCR 1 cut(s) 2298
Eam1104I CTCTTC 6 cut(s) 254, 769, 1041, 1146, 1392, 2443
Eam1105I GACNNNNNGTC 1 cut(s) 1876
EarI CTCTTC 6 cut(s) 254, 769, 1041, 1146, 1392, 2443
Eco130I CCWWGG 2 cut(s) 1125, 1521
Eco24I GRGCYC 1 cut(s) 1489
Eco31I GGTCTC 1 cut(s) 1678
Eco47I GGWCC 2 cut(s) 620, 2135
Eco57I CTGAAG 2 cut(s) 278, 681
Eco88I CYCGRG 1 cut(s) 1695
EcoO109I RGGNCCY 2 cut(s) 1485, 1486
EcoRI GAATTC 3 cut(s) 2074, 2167, 2260
EcoRII CCWGG 3 cut(s) 1710, 2557, 2574
EcoT14I CCWWGG 2 cut(s) 1125, 1521
EcoT22I ATGCAT 1 cut(s) 1350
EcoT38I GRGCYC 1 cut(s) 1489
ErhI CCWWGG 2 cut(s) 1125, 1521
FalI AAGNNNNNCTT 2 cut(s) 1265, 1297
FaqI GGGAC 2 cut(s) 662, 1743
FauNDI CATATG 1 cut(s) 757
FbaI TGATCA 1 cut(s) 478
FokI GGATG 6 cut(s) 143, 230, 395, 1207, 1691, 2045
FriOI GRGCYC 1 cut(s) 1489
FspBI CTAG 5 cut(s) 395, 1040, 1821, 1922, 2642
GlaI GCGC 2 cut(s) 527, 536
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 1458
HapII CCGG 2 cut(s) 532, 1544
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 2 cut(s) 528, 537
Hin6I GCGC 2 cut(s) 526, 535
HinP1I GCGC 2 cut(s) 526, 535
HindIII AAGCTT 1 cut(s) 2049
HinfI GANTC 4 cut(s) 446, 602, 1499, 2539
HpaII CCGG 2 cut(s) 532, 1544
HphI GGTGA 5 cut(s) 368, 469, 692, 744, 2331
Hpy166II GTNNAC 5 cut(s) 1579, 1916, 1996, 2120, 2218
Hpy8I GTNNAC 5 cut(s) 1579, 1916, 1996, 2120, 2218
Hpy99I CGWCG 1 cut(s) 2335
HpyCH4III ACNGT 4 cut(s) 597, 824, 2015, 2117
HpyCH4IV ACGT 3 cut(s) 913, 1829, 2330
HpySE526I ACGT 3 cut(s) 913, 1829, 2330
HspAI GCGC 2 cut(s) 526, 535
Kpn2I TCCGGA 1 cut(s) 1543
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Ksp22I TGATCA 1 cut(s) 478
LguI GCTCTTC 3 cut(s) 254, 1041, 1392
LmnI GCTCC 2 cut(s) 865, 1417
Lsp1109I GCAGC 6 cut(s) 265, 381, 1087, 1090, 1345, 2566
LweI GCATC 7 cut(s) 371, 554, 934, 1169, 1210, 1401, 2378
MaeI CTAG 5 cut(s) 395, 1040, 1821, 1922, 2642
MaeII ACGT 3 cut(s) 913, 1829, 2330
MaeIII GTNAC 3 cut(s) 655, 1496, 1643
MbiI CCGCTC 1 cut(s) 1420
MfeI CAATTG 3 cut(s) 1164, 2181, 2546
MflI RGATCY 3 cut(s) 1248, 1707, 1813
MhlI GDGCHC 3 cut(s) 342, 1489, 1998
MlsI TGGCCA 1 cut(s) 2300
MluNI TGGCCA 1 cut(s) 2300
MlyI GAGTC 2 cut(s) 455, 1493
MmeI TCCRAC 2 cut(s) 303, 887
Mox20I TGGCCA 1 cut(s) 2300
Mph1103I ATGCAT 1 cut(s) 1350
MreI CGCCGGCG 1 cut(s) 531
MroI TCCGGA 1 cut(s) 1543
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 2 cut(s) 2359, 2493
MscI TGGCCA 1 cut(s) 2300
MseI TTAA 5 cut(s) 143, 1622, 1826, 1989, 2429
MslI CAYNNNNRTG 5 cut(s) 306, 333, 1130, 1392, 2130
Msp20I TGGCCA 1 cut(s) 2300
MspA1I CMGCKG 2 cut(s) 256, 2500
MspI CCGG 2 cut(s) 532, 1544
MspR9I CCNGG 3 cut(s) 1712, 2559, 2576
MunI CAATTG 3 cut(s) 1164, 2181, 2546
Mva1269I GAATGC 2 cut(s) 1387, 1726
MvaI CCWGG 3 cut(s) 1712, 2559, 2576
NaeI GCCGGC 1 cut(s) 533
NcoI CCATGG 1 cut(s) 1125
NdeI CATATG 1 cut(s) 757
NgoMIV GCCGGC 1 cut(s) 531
NlaIV GGNNCC 6 cut(s) 179, 1486, 1487, 2148, 2241, 2556
NmuCI GTSAC 2 cut(s) 1496, 1643
NsiI ATGCAT 1 cut(s) 1350
NspI RCATGY 5 cut(s) 15, 1348, 1577, 1585, 2161
NspV TTCGAA 1 cut(s) 1568
OliI CACNNNNGTG 1 cut(s) 2130
PaeI GCATGC 2 cut(s) 15, 2161
PagI TCATGA 2 cut(s) 328, 1852
PciSI GCTCTTC 3 cut(s) 254, 1041, 1392
PctI GAATGC 2 cut(s) 1387, 1726
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 2 cut(s) 2359, 2493
PfeI GAWTC 2 cut(s) 602, 2539
PflMI CCANNNNNTGG 2 cut(s) 613, 692
PleI GAGTC 2 cut(s) 454, 1493
PpsI GAGTC 2 cut(s) 454, 1493
Psp6I CCWGG 3 cut(s) 1710, 2557, 2574
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 3 cut(s) 1710, 2557, 2574
PspN4I GGNNCC 6 cut(s) 179, 1486, 1487, 2148, 2241, 2556
PspOMI GGGCCC 1 cut(s) 1485
PspPI GGNCC 7 cut(s) 221, 620, 878, 1485, 1486, 2135, 2473
PstI CTGCAG 2 cut(s) 2275, 2374
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 3 cut(s) 1248, 1707, 1813
PvuII CAGCTG 2 cut(s) 256, 2500
RsaI GTAC 3 cut(s) 594, 1063, 2659
RsaNI GTAC 3 cut(s) 593, 1062, 2658
RseI CAYNNNNRTG 5 cut(s) 306, 333, 1130, 1392, 2130
SapI GCTCTTC 3 cut(s) 254, 1041, 1392
SaqAI TTAA 5 cut(s) 143, 1622, 1826, 1989, 2429
Sau96I GGNCC 7 cut(s) 221, 620, 878, 1485, 1486, 2135, 2473
SchI GAGTC 2 cut(s) 455, 1493
ScrFI CCNGG 3 cut(s) 1712, 2559, 2576
SduI GDGCHC 3 cut(s) 342, 1489, 1998
SfaNI GCATC 7 cut(s) 371, 554, 934, 1169, 1210, 1401, 2378
SfcI CTRYAG 3 cut(s) 1303, 2271, 2370
SfuI TTCGAA 1 cut(s) 1568
SgrAI CRCCGGYG 1 cut(s) 531
SinI GGWCC 2 cut(s) 620, 2135
SmiMI CAYNNNNRTG 5 cut(s) 306, 333, 1130, 1392, 2130
SmlI CTYRAG 3 cut(s) 580, 899, 2040
SmoI CTYRAG 3 cut(s) 580, 899, 2040
SphI GCATGC 2 cut(s) 15, 2161
SspMI CTAG 5 cut(s) 395, 1040, 1821, 1922, 2642
StyD4I CCNGG 3 cut(s) 1710, 2557, 2574
StyI CCWWGG 2 cut(s) 1125, 1521
TaaI ACNGT 4 cut(s) 597, 824, 2015, 2117
TaiI ACGT 3 cut(s) 916, 1832, 2333
TaqI TCGA 6 cut(s) 119, 276, 317, 505, 1568, 2105
TauI GCSGC 5 cut(s) 531, 708, 711, 1115, 2590
TfiI GAWTC 2 cut(s) 602, 2539
Tru1I TTAA 5 cut(s) 143, 1622, 1826, 1989, 2429
Tru9I TTAA 5 cut(s) 143, 1622, 1826, 1989, 2429
TscAI CASTG 5 cut(s) 46, 877, 1606, 2020, 2475
TseFI GTSAC 2 cut(s) 1496, 1643
TseI GCWGC 6 cut(s) 253, 369, 1100, 1103, 1358, 2579
Tsp45I GTSAC 2 cut(s) 1496, 1643
TspGWI ACGGA 2 cut(s) 2252, 2567
TspRI CASTG 5 cut(s) 46, 877, 1606, 2020, 2475
Van91I CCANNNNNTGG 2 cut(s) 613, 692
VneI GTGCAC 1 cut(s) 1994
VpaK11BI GGWCC 2 cut(s) 620, 2135
XapI RAATTY 6 cut(s) 460, 961, 2074, 2167, 2260, 2359
XceI RCATGY 5 cut(s) 15, 1348, 1577, 1585, 2161
XcmI CCANNNNNNNNNTGG 1 cut(s) 614
XmnI GAANNNNTTC 2 cut(s) 2359, 2493
XspI CTAG 5 cut(s) 395, 1040, 1821, 1922, 2642
Zsp2I ATGCAT 1 cut(s) 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.