pycom04g00640

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005611
Physical Location & Seq
Forward (+)
615381 .. 617326
1946 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00640.2

Sequence Viewer

Length: 1143 bp
ATGGCATGCCAAATGATACATTCGTGGAAATTTAACGAGCTAGTCGGTGCATTTCTTGATCTTGCAATAGCCTACCTTCTACTATGTGCCGCGGCAGTTGCCTACTTTACCTCGAAATTTGTTGGTCTGTTTGGGTTTTGTCTGCCGTGCCCTTGTGATGGGTTTTTTGGGACCCCAAGGAAAAGTAACTGCTTTCAGAGGCAATTTGCGGATGCCCCGTGTGAGAAAATCTCTTCAGTTCTATGGTCCGTTAGGAGTAAGTTCCCTTTCGATGTTATATGGGATGAAGACCCAAATTTCCATTCGAAGTTGAAGTCCCTAAATGAGGCAAATGGGAGTTTTGCGTTTGAAGGTGAAGCGTCATGTAGCTCATTTTCGGTGGCTGAGAATGAACAGAGTGTTGAGTATGGTGTAGGCAACTTGGCAGCTGGGAAGGAACGGAGTTTTGATCTTAAGGCAAAGAAGGTTGCAGGTCGAAGGCCGAGACTTCGGTGCCGCCGCAGAGGGGGCTCTGTTGATTATGGAAATTCATCTTCAGTTTCATCATATAATATGTTCCAGTCCGCTGCAGAGGACAATCCCACATCTCCTTCCAGCATTAGTAAAATGGGAAATGAGGTTACTGAAGTTTTTGTAAATTCAGATGGCATTGAAGCTCGTACAGATATGAGTTCGCCTGAAAGCGTTTCACGTGTTGGGTTTAATGAACATGCTGATGAAACTAAATCAGTGGAGAAGGATGGATCACTTGTTGAAGATTCTGGATCCAATGCAGGAGAGAAACTAGGTTTTGACAGTAATGAGACAACTACATTTAGAGTGCTGGAACAGGCACTCGAAGAAGAGCATGCTGCTCGTGCTGCTCTGTATCTTGAACTGGAGAAGGAGAGAAGTGCTGCTGCCACCGCTGCAGATGAGGCCATGGCCATGATAATGCGCCTGCAAGAGGAGAAGGCATCAATAGAAATGGAGGCAATGCAGTACCAAAGGATGATAGAGGAAAAATTTGCTTACGATGCTGAGGAAATGAACATTCTTACAGAGATCTTAATTAGGAGGGAGAGGGAAAAGCATTTCTTGGAGAACGAAGTTGAGCAATACAGGCAAATGTTTTTTGGAGATCGATCAAGTGGATTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

381

Amino Acids

42.47

Weight (kDa)

4.91

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 461
AccB1I GGYRCC 1 cut(s) 492
AccII CGCG 1 cut(s) 92
AciI CCGC 7 cut(s) 90, 92, 209, 496, 499, 564, 906
AclWI GGATC 3 cut(s) 751, 759, 772
AcoI YGGCCR 1 cut(s) 924
AcsI RAATTY 6 cut(s) 29, 116, 295, 526, 637, 1004
AcuI CTGAAG 3 cut(s) 219, 519, 645
AcvI CACGTG 1 cut(s) 692
AfaI GTAC 2 cut(s) 661, 983
AfiI CCNNNNNNNGG 4 cut(s) 158, 325, 505, 946
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 6 cut(s) 313, 350, 653, 755, 875, 1138
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
AluBI AGCT 4 cut(s) 40, 369, 428, 656
AluI AGCT 4 cut(s) 40, 369, 428, 656
Alw26I GTCTC 2 cut(s) 478, 797
AlwI GGATC 3 cut(s) 751, 759, 772
AoxI GGCC 3 cut(s) 479, 918, 924
ApeKI GCWGC 7 cut(s) 425, 566, 851, 860, 896, 899, 908
ApoI RAATTY 6 cut(s) 29, 116, 295, 526, 637, 1004
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
AspLEI GCGC 1 cut(s) 939
AspS9I GGNCC 2 cut(s) 171, 246
AsuHPI GGTGA 1 cut(s) 365
AsuII TTCGAA 1 cut(s) 305
AvaII GGWCC 2 cut(s) 171, 246
BaeGI GKGCMC 1 cut(s) 152
BaeI ACNNNNGTAYC 2 cut(s) 8, 41
BalI TGGCCA 1 cut(s) 926
BamHI GGATCC 1 cut(s) 764
BanI GGYRCC 1 cut(s) 492
BanII GRGCYC 1 cut(s) 512
BauI CACGAG 1 cut(s) 855
BbrPI CACGTG 1 cut(s) 692
BbsI GAAGAC 1 cut(s) 294
BbvCI CCTCAGC 1 cut(s) 1020
BbvI GCAGC 7 cut(s) 437, 553, 838, 847, 883, 886, 895
BccI CCATC 3 cut(s) 152, 638, 734
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 836, 870
BcoDI GTCTC 2 cut(s) 478, 797
BfaI CTAG 2 cut(s) 41, 785
BfmI CTRYAG 2 cut(s) 567, 909
BfrI CTTAAG 1 cut(s) 452
BfuAI ACCTGC 1 cut(s) 461
BglII AGATCT 1 cut(s) 1044
Bme18I GGWCC 2 cut(s) 171, 246
BmgT120I GGNCC 2 cut(s) 171, 246
BmiI GGNNCC 4 cut(s) 172, 173, 494, 766
BmsI GCATC 3 cut(s) 202, 965, 1006
BpiI GAAGAC 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 215, 247
BpmI CTGGAG 1 cut(s) 899
Bpu10I CCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 305
Bsa29I ATCGAT 1 cut(s) 1123
BsaAI YACGTR 1 cut(s) 692
BsaJI CCNNGG 3 cut(s) 90, 176, 921
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 325, 505, 946
Bse1I ACTGG 2 cut(s) 559, 882
Bse3DI GCAATG 1 cut(s) 981
BseCI ATCGAT 1 cut(s) 1123
BseDI CCNNGG 3 cut(s) 90, 176, 921
BseGI GGATG 4 cut(s) 217, 289, 745, 996
BseLI CCNNNNNNNGG 4 cut(s) 158, 325, 505, 946
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 2 cut(s) 375, 1011
BseNI ACTGG 2 cut(s) 559, 882
BseRI GAGGAG 1 cut(s) 962
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 7 cut(s) 437, 553, 838, 847, 883, 886, 895
BseYI CCCAGC 1 cut(s) 428
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 3 cut(s) 481, 920, 926
BshNI GGYRCC 1 cut(s) 492
BshVI ATCGAT 1 cut(s) 1123
BslFI GGGAC 2 cut(s) 184, 301
BslI CCNNNNNNNGG 4 cut(s) 158, 325, 505, 946
BsmAI GTCTC 2 cut(s) 478, 797
BsmFI GGGAC 2 cut(s) 184, 301
BsnI GGCC 3 cut(s) 481, 920, 926
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 152, 512
Bsp143I GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 7 cut(s) 90, 92, 209, 496, 499, 564, 906
BspANI GGCC 3 cut(s) 481, 920, 926
BspCNI CTCAG 2 cut(s) 376, 1012
BspDI ATCGAT 1 cut(s) 1123
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 4 cut(s) 172, 173, 494, 766
BspMAI CTGCAG 2 cut(s) 571, 913
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 3 cut(s) 751, 759, 772
BspQI GCTCTTC 1 cut(s) 837
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 492
BspTI CTTAAG 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 981
BsrI ACTGG 2 cut(s) 559, 882
BssECI CCNNGG 3 cut(s) 90, 176, 921
BssMI GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 2 cut(s) 176, 921
Bst2BI CACGAG 1 cut(s) 855
Bst4CI ACNGT 1 cut(s) 797
Bst6I CTCTTC 2 cut(s) 238, 837
BstAFI CTTAAG 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 692
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 3 cut(s) 7, 849, 941
BstDEI CTNAG 2 cut(s) 384, 1020
BstDSI CCRYGG 2 cut(s) 90, 921
BstENI CCTNNNNNAGG 2 cut(s) 323, 944
BstF5I GGATG 4 cut(s) 217, 289, 745, 996
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 1 cut(s) 939
BstKTI GATC 7 cut(s) 61, 451, 746, 767, 1047, 1123, 1127
BstMAI GTCTC 2 cut(s) 478, 797
BstMBI GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
BstMWI GCNNNNNNNGC 9 cut(s) 98, 507, 857, 860, 905, 908, 917, 1016, 1102
BstNSI RCATGY 3 cut(s) 9, 713, 851
BstSFI CTRYAG 2 cut(s) 567, 909
BstSLI GKGCMC 1 cut(s) 152
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 7 cut(s) 437, 553, 838, 847, 883, 886, 895
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 2 cut(s) 764, 1044
BstYI RGATCY 2 cut(s) 764, 1044
Bsu15I ATCGAT 1 cut(s) 1123
BsuRI GGCC 3 cut(s) 481, 920, 926
BsuTUI ATCGAT 1 cut(s) 1123
BtgI CCRYGG 2 cut(s) 90, 921
BtsCI GGATG 4 cut(s) 217, 289, 745, 996
BtsIMutI CAGTG 1 cut(s) 735
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 3 cut(s) 7, 849, 941
CfoI GCGC 1 cut(s) 939
Cfr13I GGNCC 2 cut(s) 171, 246
Cfr42I CCGCGG 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 1123
CseI GACGC 1 cut(s) 348
Csp6I GTAC 2 cut(s) 660, 982
CviAII CATG 6 cut(s) 6, 363, 710, 848, 922, 928
CviQI GTAC 2 cut(s) 660, 982
DdeI CTNAG 2 cut(s) 384, 1020
DpnI GATC 7 cut(s) 60, 450, 745, 766, 1046, 1122, 1126
DpnII GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
EaeI YGGCCR 1 cut(s) 924
Eam1104I CTCTTC 2 cut(s) 238, 837
EarI CTCTTC 2 cut(s) 238, 837
Eco130I CCWWGG 2 cut(s) 176, 921
Eco24I GRGCYC 1 cut(s) 512
Eco47I GGWCC 2 cut(s) 171, 246
Eco57I CTGAAG 3 cut(s) 219, 519, 645
Eco72I CACGTG 1 cut(s) 692
EcoNI CCTNNNNNAGG 2 cut(s) 323, 944
EcoO109I RGGNCCY 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 176, 921
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 2 cut(s) 176, 921
FaeI CATG 6 cut(s) 9, 366, 713, 851, 925, 931
FalI AAGNNNNNCTT 2 cut(s) 1061, 1093
FaqI GGGAC 2 cut(s) 184, 301
FatI CATG 6 cut(s) 5, 362, 709, 847, 921, 927
FokI GGATG 4 cut(s) 224, 296, 752, 1003
FriOI GRGCYC 1 cut(s) 512
FspBI CTAG 2 cut(s) 41, 785
GlaI GCGC 1 cut(s) 938
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 899
HaeIII GGCC 3 cut(s) 481, 920, 926
HgaI GACGC 1 cut(s) 348
HhaI GCGC 1 cut(s) 939
Hin1II CATG 6 cut(s) 9, 366, 713, 851, 925, 931
Hin6I GCGC 1 cut(s) 937
HinP1I GCGC 1 cut(s) 937
HinfI GANTC 2 cut(s) 758, 1134
HphI GGTGA 1 cut(s) 365
Hpy188I TCNGA 2 cut(s) 198, 643
Hpy188III TCNNGA 3 cut(s) 56, 762, 872
HpyAV CCTTC 9 cut(s) 86, 344, 427, 457, 471, 600, 730, 877, 946
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 1 cut(s) 691
HpyCH4V TGCA 8 cut(s) 50, 65, 470, 569, 773, 911, 943, 979
HpyF10VI GCNNNNNNNGC 9 cut(s) 98, 507, 857, 860, 905, 908, 917, 1016, 1102
HpyF3I CTNAG 2 cut(s) 384, 1020
HpySE526I ACGT 1 cut(s) 691
Hsp92II CATG 6 cut(s) 9, 366, 713, 851, 925, 931
HspAI GCGC 1 cut(s) 937
KflI GGGWCCC 1 cut(s) 171
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
LguI GCTCTTC 1 cut(s) 837
Lsp1109I GCAGC 7 cut(s) 437, 553, 838, 847, 883, 886, 895
LweI GCATC 3 cut(s) 202, 965, 1006
MaeI CTAG 2 cut(s) 41, 785
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 2 cut(s) 185, 619
MalI GATC 7 cut(s) 60, 450, 745, 766, 1046, 1122, 1126
MboI GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
MboII GAAGA 6 cut(s) 225, 299, 525, 767, 851, 854
MflI RGATCY 2 cut(s) 764, 1044
MhlI GDGCHC 2 cut(s) 152, 512
MlsI TGGCCA 1 cut(s) 926
MluCI AATT 8 cut(s) 29, 116, 203, 295, 526, 637, 1004, 1050
MluNI TGGCCA 1 cut(s) 926
Mox20I TGGCCA 1 cut(s) 926
MscI TGGCCA 1 cut(s) 926
MseI TTAA 4 cut(s) 33, 453, 702, 1049
MslI CAYNNNNRTG 3 cut(s) 714, 926, 932
Msp20I TGGCCA 1 cut(s) 926
MspA1I CMGCKG 4 cut(s) 92, 428, 566, 908
MspCI CTTAAG 1 cut(s) 452
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 9 cut(s) 98, 507, 857, 860, 905, 908, 917, 1016, 1102
NcoI CCATGG 1 cut(s) 921
NdeII GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
NlaIII CATG 6 cut(s) 9, 366, 713, 851, 925, 931
NlaIV GGNNCC 4 cut(s) 172, 173, 494, 766
NmeAIII GCCGAG 1 cut(s) 507
NspI RCATGY 3 cut(s) 9, 713, 851
NspV TTCGAA 1 cut(s) 305
PaeI GCATGC 2 cut(s) 9, 851
PciSI GCTCTTC 1 cut(s) 837
PfeI GAWTC 2 cut(s) 758, 1134
PmaCI CACGTG 1 cut(s) 692
PmlI CACGTG 1 cut(s) 692
Ppu21I YACGTR 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 171
Psp5II RGGWCCY 1 cut(s) 171
PspCI CACGTG 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 428
PspN4I GGNNCC 4 cut(s) 172, 173, 494, 766
PspPI GGNCC 2 cut(s) 171, 246
PspPPI RGGWCCY 1 cut(s) 171
PstI CTGCAG 2 cut(s) 571, 913
PsuI RGATCY 2 cut(s) 764, 1044
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 2 cut(s) 661, 983
RsaNI GTAC 2 cut(s) 660, 982
RseI CAYNNNNRTG 3 cut(s) 714, 926, 932
SacII CCGCGG 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 837
SaqAI TTAA 4 cut(s) 33, 453, 702, 1049
Sau3AI GATC 7 cut(s) 58, 448, 743, 764, 1044, 1120, 1124
Sau96I GGNCC 2 cut(s) 171, 246
SduI GDGCHC 2 cut(s) 152, 512
SfaNI GCATC 3 cut(s) 202, 965, 1006
SfcI CTRYAG 2 cut(s) 567, 909
Sfr303I CCGCGG 1 cut(s) 93
SfuI TTCGAA 1 cut(s) 305
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 2 cut(s) 171, 246
SmiMI CAYNNNNRTG 3 cut(s) 714, 926, 932
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SphI GCATGC 2 cut(s) 9, 851
Sse9I AATT 8 cut(s) 29, 116, 203, 295, 526, 637, 1004, 1050
SsiI CCGC 7 cut(s) 90, 92, 209, 496, 499, 564, 906
SspMI CTAG 2 cut(s) 41, 785
StyI CCWWGG 2 cut(s) 176, 921
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 1 cut(s) 694
TaqI TCGA 6 cut(s) 113, 270, 305, 475, 837, 1123
TasI AATT 8 cut(s) 29, 116, 203, 295, 526, 637, 1004, 1050
TauI GCSGC 4 cut(s) 92, 95, 498, 501
TfiI GAWTC 2 cut(s) 758, 1134
Tru1I TTAA 4 cut(s) 33, 453, 702, 1049
Tru9I TTAA 4 cut(s) 33, 453, 702, 1049
TscAI CASTG 1 cut(s) 735
TseI GCWGC 7 cut(s) 425, 566, 851, 860, 896, 899, 908
TspDTI ATGAA 7 cut(s) 300, 405, 519, 531, 720, 732, 1043
TspGWI ACGGA 2 cut(s) 238, 454
TspRI CASTG 1 cut(s) 735
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 2 cut(s) 171, 246
XagI CCTNNNNNAGG 2 cut(s) 323, 944
XapI RAATTY 6 cut(s) 29, 116, 295, 526, 637, 1004
XceI RCATGY 3 cut(s) 9, 713, 851
XspI CTAG 2 cut(s) 41, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.