Rorug04G0133100

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005611
Physical Location & Seq
Reverse (-)
21395543 .. 21400720
5178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0133100.1

Sequence Viewer

Length: 1611 bp
ATGTCTCCCTTTCACTTCACCTTCCGCTCCGTCCTAAACCCCCACCACCACCGCCACGTCAGCCGCAGAACCTTCGTGGACACCACCATAAACTGGGTCCGCGACCGCGACCGCGGCCTCGACCACGTGGTCGAGCGGGAGAGAAACCTCCGGCCAATCATCAACATCAAGAACCTCATCAAATCAGAGCCCTCCAAATCCCTCCCCATTTCCCTCCTCACCAAATCCCGCCACTCCCTCACGATCCCCACGCGCCCCGTCGAGTTCGTCCGACAATACCCCTCCATTTTCGAGGAGTTCCTCCCCGCCGGCATCGCCGCCGTCCAGCCCCACGTCCGCCTCACCCCGCAAGTCCTCGACATCGACGCCAACGAGCAGTTGGTGTATCAGAGCGTGACCTACACGCACCAAGCCGCCGATCGCCTCTTCAAGCTGCTAATGCTGGTGAGAAGCAACAAACTGCCGCTGAATCTCATTGAGCTTTTCAAATGGGATTTGGGGCTTCCTCAAGATTACGTGGACACCATTGTGCCGGAGTTTCCGGATTACTTTAAGATTGTAGTGGGGAAATATGGGCAGCCGGAGTTGGAGATGGTGTGTTGGAGTGATGAGCTGGCCACTTCGGTTATGGAGAAGAAGGCAAAACAGGGCTATGTCAAGGGAATGCCGCTTGCTTTTCCTGTGCAGTTTTCGAGAGGTTTCGAGATGGATAAGAAGATGAAGAAGTGGATTGATGAGTGGCAGAAGTTGCCGTATGTGTCTCCTTATGAGAATGCAGCTCATCTGTCGTCTCAGAGTGACGAGTCGGATAAGTGGGCCGTTGCGGTTTTGCATGAGCTTCTGCATATTTTGGTTCCGAAGAAGACAGACAGGGAGAATGTTTTGGCTCTGGGAGAGTATTTGGGGATTAGATCGAGGTTTAAGCGGGCATTGCTTCACCATCCGGGAATATTGTATGTGTCGAGCAAGATTAGGACTTATACAGTTGTGTTGAGAGAAGGGTATAAGAGGGGTGCCATTATCGAAAATCATCCGCTGATGGACATAAGAAACCAGTATATTCACCTCATGAATGCTGTGAAGGAAGATGGTAAAACGATAAGTTCTAAGGAGAAAAAGAAGAAGAAGGGAATTGAAGATTCTAAAGGAGAGGATGTAGAAGAGGACGACGATGACAATGAGGAAGCGATGGAAGGGGAGTTGTGTGATTCTTCTGATGCTGAAGTGGAGCATGACAATGTTGACAGTGAAGACGATGAAGAAGAAGATGAAGACGATGATGAAGAAGAGAGTATGACAAGCGTTCGAATGAATGATGCTAATACTAGAGGAAGAAGAGCTAGGAAATCTAATTTTGATGTCATGACACCGTCCAGAAATGCTGGAAGAAGAGGAAGTTCAGGTCAAAGAAGAGCTTGGAAATCTGATAATACTGAGAGAGGGAGGTCTGGTGGACAATACCCTGGTAGGAGTGGGAACGAAGTCTCAGCAGAAACTTCTAGAAGAACACAAGCTGATGTTAGGCATAATGTCCACCAAAATTCAAGAGAAACATCAAATCGCTCCAGAAGCAGGGGGAGTTCTTCACCAGACAAGAAGAATTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

536

Amino Acids

61.73

Weight (kDa)

6.58

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 36 - 360 2.7e-114 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 1013
AccB7I CCANNNNNTGG 1 cut(s) 93
AccBSI CCGCTC 2 cut(s) 27, 136
AccII CGCG 4 cut(s) 102, 108, 114, 253
AccIII TCCGGA 1 cut(s) 541
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 2 cut(s) 152, 615
AcsI RAATTY 1 cut(s) 1540
AcuI CTGAAG 1 cut(s) 1242
AcvI CACGTG 1 cut(s) 127
AcyI GRCGYC 1 cut(s) 366
AfiI CCNNNNNNNGG 2 cut(s) 93, 1572
AgsI TTSAA 4 cut(s) 430, 487, 1136, 1545
AjiI CACGTC 2 cut(s) 58, 334
AjnI CCWGG 1 cut(s) 1462
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 8 cut(s) 433, 481, 613, 779, 838, 1340, 1415, 1514
AluI AGCT 8 cut(s) 433, 481, 613, 779, 838, 1340, 1415, 1514
Alw26I GTCTC 4 cut(s) 9, 765, 795, 1489
AlwI GGATC 1 cut(s) 238
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 4 cut(s) 115, 152, 615, 816
ApeKI GCWGC 3 cut(s) 433, 577, 776
ApoI RAATTY 1 cut(s) 1540
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 2 cut(s) 97, 816
AsuC2I CCSGG 1 cut(s) 945
AsuHPI GGTGA 7 cut(s) 10, 211, 334, 457, 929, 1055, 1578
AsuII TTCGAA 1 cut(s) 1306
AvaII GGWCC 1 cut(s) 97
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 1 cut(s) 1013
BanII GRGCYC 1 cut(s) 192
BbrPI CACGTG 1 cut(s) 127
BbsI GAAGAC 3 cut(s) 869, 1257, 1278
BbvI GCAGC 3 cut(s) 420, 589, 788
BccI CCATC 6 cut(s) 586, 700, 948, 1033, 1082, 1183
BceAI ACGGC 3 cut(s) 305, 736, 803
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 1 cut(s) 1464
BcnI CCSGG 1 cut(s) 945
BcoDI GTCTC 4 cut(s) 9, 765, 795, 1489
BfaI CTAG 3 cut(s) 1326, 1341, 1500
BisI GCNGC 9 cut(s) 64, 115, 318, 414, 434, 464, 578, 668, 777
BlsI GCNGC 9 cut(s) 65, 116, 319, 415, 435, 465, 579, 669, 778
Bme1390I CCNGG 2 cut(s) 945, 1464
Bme18I GGWCC 1 cut(s) 97
BmgBI CACGTC 2 cut(s) 58, 334
BmgT120I GGNCC 2 cut(s) 97, 816
BmiI GGNNCC 3 cut(s) 98, 855, 1015
BmrFI CCNGG 2 cut(s) 945, 1464
BmrI ACTGGG 1 cut(s) 103
BmsI GCATC 3 cut(s) 321, 1207, 1306
BmuI ACTGGG 1 cut(s) 103
BpiI GAAGAC 3 cut(s) 869, 1257, 1278
BpmI CTGGAG 1 cut(s) 1549
Bpu14I TTCGAA 1 cut(s) 1306
BpuEI CTTGAG 1 cut(s) 492
BpuMI CCSGG 1 cut(s) 945
BsaAI YACGTR 2 cut(s) 127, 517
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 112, 1462
BsaWI WCCGGW 1 cut(s) 541
BsaXI ACNNNNNCTCC 6 cut(s) 581, 611, 1140, 1170, 1435, 1465
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 1572
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 98, 1054
Bse3DI GCAATG 1 cut(s) 929
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 1 cut(s) 1464
BseDI CCNNGG 2 cut(s) 112, 1462
BseGI GGATG 3 cut(s) 940, 1030, 1159
BseLI CCNNNNNNNGG 2 cut(s) 93, 1572
BseMI GCAATG 1 cut(s) 929
BseMII CTCAG 3 cut(s) 806, 1425, 1500
BseNI ACTGG 2 cut(s) 98, 1054
BseRI GAGGAG 2 cut(s) 206, 308
BseXI GCAGC 3 cut(s) 420, 589, 788
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 4 cut(s) 102, 108, 114, 253
Bsh1285I CGRYCG 3 cut(s) 106, 112, 421
BshFI GGCC 4 cut(s) 117, 154, 617, 818
BshNI GGYRCC 1 cut(s) 1013
BsiEI CGRYCG 3 cut(s) 106, 112, 421
BsiSI CCGG 6 cut(s) 151, 309, 533, 542, 581, 944
BslI CCNNNNNNNGG 2 cut(s) 93, 1572
BsmAI GTCTC 4 cut(s) 9, 765, 795, 1489
BsmBI CGTCTC 1 cut(s) 795
BsmI GAATGC 3 cut(s) 669, 778, 1078
BsnI GGCC 4 cut(s) 117, 154, 617, 818
Bsp119I TTCGAA 1 cut(s) 1306
Bsp1286I GDGCHC 1 cut(s) 192
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 3 cut(s) 243, 418, 911
BspANI GGCC 4 cut(s) 117, 154, 617, 818
BspCNI CTCAG 3 cut(s) 805, 1426, 1499
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 4 cut(s) 102, 108, 114, 253
BspHI TCATGA 2 cut(s) 1068, 1362
BspLI GGNNCC 3 cut(s) 98, 855, 1015
BspPI GGATC 1 cut(s) 238
BspQI GCTCTTC 2 cut(s) 1330, 1405
BspT104I TTCGAA 1 cut(s) 1306
BspT107I GGYRCC 1 cut(s) 1013
BsrBI CCGCTC 2 cut(s) 27, 136
BsrDI GCAATG 1 cut(s) 929
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 2 cut(s) 98, 1054
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 2 cut(s) 112, 1462
BssMI GATC 3 cut(s) 243, 418, 911
BssNI GRCGYC 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 1464
Bst4CI ACNGT 3 cut(s) 985, 1247, 1371
Bst6I CTCTTC 6 cut(s) 431, 1155, 1281, 1330, 1384, 1405
BstACI GRCGYC 1 cut(s) 366
BstAPI GCANNNNNTGC 1 cut(s) 748
BstBAI YACGTR 2 cut(s) 127, 517
BstBI TTCGAA 1 cut(s) 1306
BstC8I GCNNGC 4 cut(s) 310, 615, 672, 927
BstDEI CTNAG 4 cut(s) 792, 1107, 1434, 1486
BstDSI CCRYGG 1 cut(s) 112
BstF5I GGATG 3 cut(s) 940, 1030, 1159
BstFNI CGCG 4 cut(s) 102, 108, 114, 253
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 3 cut(s) 246, 421, 914
BstMAI GTCTC 4 cut(s) 9, 765, 795, 1489
BstMBI GATC 3 cut(s) 243, 418, 911
BstMCI CGRYCG 3 cut(s) 106, 112, 421
BstMWI GCNNNNNNNGC 7 cut(s) 60, 114, 314, 439, 748, 931, 1569
BstNI CCWGG 1 cut(s) 1464
BstSCI CCNGG 2 cut(s) 943, 1462
BstUI CGCG 4 cut(s) 102, 108, 114, 253
BstV1I GCAGC 3 cut(s) 420, 589, 788
BstV2I GAAGAC 3 cut(s) 869, 1257, 1278
BsuRI GGCC 4 cut(s) 117, 154, 617, 818
BtgI CCRYGG 1 cut(s) 112
BtgZI GCGATG 2 cut(s) 298, 1202
BtrI CACGTC 2 cut(s) 58, 334
BtsCI GGATG 3 cut(s) 940, 1030, 1159
BtsIMutI CAGTG 1 cut(s) 1252
Cac8I GCNNGC 4 cut(s) 310, 615, 672, 927
CciI TCATGA 2 cut(s) 1068, 1362
CfoI GCGC 1 cut(s) 255
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 97, 816
Cfr42I CCGCGG 1 cut(s) 115
CseI GACGC 1 cut(s) 374
CviAII CATG 4 cut(s) 833, 1069, 1232, 1363
DdeI CTNAG 4 cut(s) 792, 1107, 1434, 1486
DpnI GATC 3 cut(s) 245, 420, 913
DpnII GATC 3 cut(s) 243, 418, 911
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 2 cut(s) 152, 615
Eam1104I CTCTTC 6 cut(s) 431, 1155, 1281, 1330, 1384, 1405
EarI CTCTTC 6 cut(s) 431, 1155, 1281, 1330, 1384, 1405
EciI GGCGGA 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 1242
Eco72I CACGTG 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 1462
EcoT38I GRGCYC 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 795
FaeI CATG 4 cut(s) 836, 1072, 1235, 1366
FalI AAGNNNNNCTT 2 cut(s) 1399, 1431
FatI CATG 4 cut(s) 832, 1068, 1231, 1362
FauI CCCGC 5 cut(s) 129, 236, 313, 354, 918
Fnu4HI GCNGC 9 cut(s) 64, 115, 318, 414, 434, 464, 578, 668, 777
FokI GGATG 3 cut(s) 927, 1017, 1166
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 9 cut(s) 64, 115, 318, 414, 434, 464, 578, 668, 777
FspBI CTAG 3 cut(s) 1326, 1341, 1500
GlaI GCGC 1 cut(s) 254
GluI GCNGC 9 cut(s) 64, 115, 318, 414, 434, 464, 578, 668, 777
GsuI CTGGAG 1 cut(s) 1549
HaeIII GGCC 4 cut(s) 117, 154, 617, 818
HapII CCGG 6 cut(s) 151, 309, 533, 542, 581, 944
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 255
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 4 cut(s) 836, 1072, 1235, 1366
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HincII GTYRAC 1 cut(s) 1243
HindII GTYRAC 1 cut(s) 1243
HinfI GANTC 4 cut(s) 469, 803, 1139, 1208
HpaII CCGG 6 cut(s) 151, 309, 533, 542, 581, 944
HphI GGTGA 7 cut(s) 10, 211, 334, 457, 929, 1055, 1578
Hpy166II GTNNAC 5 cut(s) 79, 520, 1243, 1454, 1534
Hpy188I TCNGA 8 cut(s) 187, 272, 390, 795, 808, 858, 1216, 1426
Hpy8I GTNNAC 5 cut(s) 79, 520, 1243, 1454, 1534
Hpy99I CGWCG 3 cut(s) 263, 368, 1172
HpyAV CCTTC 7 cut(s) 31, 82, 631, 992, 1075, 1120, 1187
HpyCH4III ACNGT 3 cut(s) 985, 1247, 1371
HpyCH4IV ACGT 4 cut(s) 57, 126, 333, 516
HpyCH4V TGCA 4 cut(s) 685, 776, 832, 844
HpyF10VI GCNNNNNNNGC 7 cut(s) 60, 114, 314, 439, 748, 931, 1569
HpyF3I CTNAG 4 cut(s) 792, 1107, 1434, 1486
HpySE526I ACGT 4 cut(s) 57, 126, 333, 516
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 4 cut(s) 836, 1072, 1235, 1366
HspAI GCGC 1 cut(s) 253
Kpn2I TCCGGA 1 cut(s) 541
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
KspI CCGCGG 1 cut(s) 115
Kzo9I GATC 3 cut(s) 243, 418, 911
LguI GCTCTTC 2 cut(s) 1330, 1405
LmnI GCTCC 3 cut(s) 32, 1228, 1568
Lsp1109I GCAGC 3 cut(s) 420, 589, 788
LweI GCATC 3 cut(s) 321, 1207, 1306
MaeI CTAG 3 cut(s) 1326, 1341, 1500
MaeII ACGT 4 cut(s) 57, 126, 333, 516
MaeIII GTNAC 2 cut(s) 394, 797
MalI GATC 3 cut(s) 245, 420, 913
MbiI CCGCTC 2 cut(s) 27, 136
MboI GATC 3 cut(s) 243, 418, 911
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 4 cut(s) 1131, 1351, 1540, 1600
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 1 cut(s) 812
MmeI TCCRAC 4 cut(s) 295, 567, 581, 786
Mox20I TGGCCA 1 cut(s) 617
MroI TCCGGA 1 cut(s) 541
MroNI GCCGGC 1 cut(s) 308
MscI TGGCCA 1 cut(s) 617
MseI TTAA 2 cut(s) 552, 921
MslI CAYNNNNRTG 2 cut(s) 527, 1236
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 3 cut(s) 114, 466, 1036
MspI CCGG 6 cut(s) 151, 309, 533, 542, 581, 944
MspR9I CCNGG 2 cut(s) 945, 1464
Mva1269I GAATGC 3 cut(s) 669, 778, 1078
MvaI CCWGG 1 cut(s) 1464
MvnI CGCG 4 cut(s) 102, 108, 114, 253
MwoI GCNNNNNNNGC 7 cut(s) 60, 114, 314, 439, 748, 931, 1569
NaeI GCCGGC 1 cut(s) 310
NciI CCSGG 1 cut(s) 945
NdeII GATC 3 cut(s) 243, 418, 911
NgoMIV GCCGGC 1 cut(s) 308
NlaIII CATG 4 cut(s) 836, 1072, 1235, 1366
NlaIV GGNNCC 3 cut(s) 98, 855, 1015
NmuCI GTSAC 2 cut(s) 394, 797
NspV TTCGAA 1 cut(s) 1306
OliI CACNNNNGTG 1 cut(s) 527
PagI TCATGA 2 cut(s) 1068, 1362
PciSI GCTCTTC 2 cut(s) 1330, 1405
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PctI GAATGC 3 cut(s) 669, 778, 1078
PdiI GCCGGC 1 cut(s) 310
PfeI GAWTC 3 cut(s) 469, 1139, 1208
PflFI GACNNNGTC 1 cut(s) 1369
PflMI CCANNNNNTGG 1 cut(s) 93
PfoI TCCNGGA 1 cut(s) 943
PkrI GCNGC 9 cut(s) 65, 116, 319, 415, 435, 465, 579, 669, 778
Ple19I CGATCG 1 cut(s) 421
PleI GAGTC 1 cut(s) 811
PmaCI CACGTG 1 cut(s) 127
PmlI CACGTG 1 cut(s) 127
PpsI GAGTC 1 cut(s) 811
Ppu21I YACGTR 2 cut(s) 127, 517
Psp6I CCWGG 1 cut(s) 1462
PspCI CACGTG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 1462
PspN4I GGNNCC 3 cut(s) 98, 855, 1015
PspPI GGNCC 2 cut(s) 97, 816
PsyI GACNNNGTC 1 cut(s) 1369
PvuI CGATCG 1 cut(s) 421
RseI CAYNNNNRTG 2 cut(s) 527, 1236
SacII CCGCGG 1 cut(s) 115
SapI GCTCTTC 2 cut(s) 1330, 1405
SaqAI TTAA 2 cut(s) 552, 921
SatI GCNGC 9 cut(s) 64, 115, 318, 414, 434, 464, 578, 668, 777
Sau3AI GATC 3 cut(s) 243, 418, 911
Sau96I GGNCC 2 cut(s) 97, 816
SchI GAGTC 1 cut(s) 812
ScrFI CCNGG 2 cut(s) 945, 1464
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 3 cut(s) 321, 1207, 1306
Sfr303I CCGCGG 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 1306
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 1 cut(s) 97
SmiMI CAYNNNNRTG 2 cut(s) 527, 1236
SmlI CTYRAG 1 cut(s) 507
SmoI CTYRAG 1 cut(s) 507
Sse9I AATT 4 cut(s) 1131, 1351, 1540, 1600
SspI AATATT 1 cut(s) 951
SspMI CTAG 3 cut(s) 1326, 1341, 1500
StyD4I CCNGG 2 cut(s) 943, 1462
TaaI ACNGT 3 cut(s) 985, 1247, 1371
TaiI ACGT 4 cut(s) 60, 129, 336, 519
TasI AATT 4 cut(s) 1131, 1351, 1540, 1600
TauI GCSGC 6 cut(s) 66, 117, 320, 416, 466, 670
TfiI GAWTC 3 cut(s) 469, 1139, 1208
Tru1I TTAA 2 cut(s) 552, 921
Tru9I TTAA 2 cut(s) 552, 921
TscAI CASTG 1 cut(s) 1252
TseFI GTSAC 2 cut(s) 394, 797
TseI GCWGC 3 cut(s) 433, 577, 776
Tsp45I GTSAC 2 cut(s) 394, 797
TspDTI ATGAA 6 cut(s) 734, 1085, 1272, 1284, 1296, 1325
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 1 cut(s) 1252
Tth111I GACNNNGTC 1 cut(s) 1369
Van91I CCANNNNNTGG 1 cut(s) 93
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 1 cut(s) 1540
XbaI TCTAGA 1 cut(s) 1499
XcmI CCANNNNNNNNNTGG 2 cut(s) 376, 625
XspI CTAG 3 cut(s) 1326, 1341, 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.